Rmu_sc0000258.1_g000014

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000258.1
OG0021628
Physical Location & Seq
Reverse (-)
68564 .. 72403
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000258.1_g000014.1.cds

Sequence Viewer

Length: 822 bp
atgggatgctcatcaaagctttgcaccatttcatgtgtcaactggtttgaagactcaacttattactacaattttttgggattaaaggagaatttcccagctgatatccttcaaaccgcaagagttttggcacagagggatgtcttgacatgtaacttatatcttgacaccatggcagattactacattgtcctctactttgctgggattctacctatgtttccttcagtcgacatcctgcttaatggtgatgtagcaaaatcaaattatactgcgccgttgccggacgggacagaattgaccttgtgtccggagagtggtccggtcggagtagtggttgcggggcgacggagatgcacaccagagagccggactgcgtacggtagaggcgggaagacggcaggggtttgggaggcgacaattcacttgttgcttcctgggtttgggaggagcatggagggcttgctcggtgtttcgagatcggatcggttgatcccgatctgggttgagtgtttcgttttcgacatgaggaaggacggcgtggcttgctggcggaaggtaggagacggaggtgcgccggcgagatgcgaaattatctctttacttggtaacttttggtggggatcaagtgagagaggccacaagatccattggaagagctggaaaagttcagggcttgcaaaggatgagggaggtatgggcttcagagagttacaagatttcaacatggcacttccagccaaacaatgctggagattaatacaagatcctcaatctctatgggcaagagttcttaaaggccgatactttcctggactgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

273

Amino Acids

30.46

Weight (kDa)

8.29

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 231
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 4 cut(s) 117, 341, 390, 553
AclWI GGATC 5 cut(s) 487, 492, 631, 640, 761
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 210, 688
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 3 cut(s) 317, 443, 502
AflIII ACRYGT 1 cut(s) 149
AgsI TTSAA 3 cut(s) 50, 113, 724
AhdI GACNNNNNGTC 1 cut(s) 306
AjnI CCWGG 2 cut(s) 436, 811
AluBI AGCT 3 cut(s) 19, 101, 660
AluI AGCT 3 cut(s) 19, 101, 660
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 5 cut(s) 487, 492, 631, 640, 761
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 2 cut(s) 637, 799
ApoI RAATTY 1 cut(s) 91
AseI ATTAAT 1 cut(s) 758
AspLEI GCGC 2 cut(s) 277, 577
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 57, 401
BceAI ACGGC 3 cut(s) 262, 414, 553
BcgI CGANNNNNNTGC 2 cut(s) 336, 370
BciT130I CCWGG 2 cut(s) 438, 813
BcoDI GTCTC 1 cut(s) 558
Bme1390I CCNGG 2 cut(s) 438, 813
Bme18I GGWCC 1 cut(s) 320
BmeRI GACNNNNNGTC 1 cut(s) 306
BmgT120I GGNCC 1 cut(s) 320
BmrFI CCNGG 2 cut(s) 438, 813
BmsI GCATC 2 cut(s) 344, 575
BpiI GAAGAC 2 cut(s) 57, 401
BpmI CTGGAG 1 cut(s) 772
BsaBI GATNNNNATC 2 cut(s) 10, 497
BsaJI CCNNGG 2 cut(s) 171, 437
BsaWI WCCGGW 2 cut(s) 310, 322
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 443, 502
Bse118I RCCGGY 1 cut(s) 577
Bse1I ACTGG 1 cut(s) 47
Bse8I GATNNNNATC 2 cut(s) 10, 497
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 2 cut(s) 438, 813
BseDI CCNNGG 2 cut(s) 171, 437
BseGI GGATG 4 cut(s) 11, 145, 234, 691
BseJI GATNNNNATC 2 cut(s) 10, 497
BseLI CCNNNNNNNGG 3 cut(s) 317, 443, 502
BseNI ACTGG 1 cut(s) 47
BseRI GAGGAG 1 cut(s) 463
BseYI CCCAGC 2 cut(s) 97, 203
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 2 cut(s) 639, 801
BsiEI CGRYCG 1 cut(s) 327
BsiSI CCGG 5 cut(s) 284, 311, 323, 370, 578
BsiWI CGTACG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 3 cut(s) 317, 443, 502
BsmAI GTCTC 1 cut(s) 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 2 cut(s) 639, 801
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 4 cut(s) 117, 341, 390, 553
BspANI GGCC 2 cut(s) 639, 801
BspEI TCCGGA 1 cut(s) 310
BspPI GGATC 5 cut(s) 487, 492, 631, 640, 761
BspQI GCTCTTC 1 cut(s) 650
BsrFI RCCGGY 1 cut(s) 577
BsrI ACTGG 1 cut(s) 47
BssAI RCCGGY 1 cut(s) 577
BssECI CCNNGG 2 cut(s) 171, 437
BssMI GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 438, 813
Bst4CI ACNGT 2 cut(s) 383, 819
Bst6I CTCTTC 1 cut(s) 650
BstC8I GCNNGC 5 cut(s) 464, 547, 551, 579, 678
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 4 cut(s) 11, 145, 234, 691
BstHHI GCGC 2 cut(s) 277, 577
BstKTI GATC 7 cut(s) 482, 487, 495, 501, 626, 648, 769
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 3 cut(s) 459, 546, 737
BstNI CCWGG 2 cut(s) 438, 813
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 2 cut(s) 436, 811
BstV2I GAAGAC 2 cut(s) 57, 401
BstX2I RGATCY 2 cut(s) 645, 766
BstYI RGATCY 2 cut(s) 645, 766
BsuRI GGCC 2 cut(s) 639, 801
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 4 cut(s) 11, 145, 234, 691
Cac8I GCNNGC 5 cut(s) 464, 547, 551, 579, 678
CfoI GCGC 2 cut(s) 277, 577
Cfr10I RCCGGY 1 cut(s) 577
Cfr13I GGNCC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 379
CviAII CATG 6 cut(s) 33, 150, 172, 454, 526, 727
CviQI GTAC 1 cut(s) 379
DpnI GATC 7 cut(s) 481, 486, 494, 500, 625, 647, 768
DpnII GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
DriI GACNNNNNGTC 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 650
Eam1105I GACNNNNNGTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 650
EciI GGCGGA 1 cut(s) 568
Eco130I CCWWGG 1 cut(s) 171
Eco32I GATATC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 2 cut(s) 210, 688
EcoRII CCWGG 2 cut(s) 436, 811
EcoRV GATATC 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 6 cut(s) 36, 153, 175, 457, 529, 730
FaqI GGGAC 1 cut(s) 304
FatI CATG 6 cut(s) 32, 149, 171, 453, 525, 726
FauI CCCGC 2 cut(s) 334, 383
FblI GTMKAC 1 cut(s) 231
FokI GGATG 4 cut(s) 18, 152, 221, 698
GlaI GCGC 2 cut(s) 276, 576
GsaI CCCAGC 2 cut(s) 101, 207
GsuI CTGGAG 1 cut(s) 772
HaeIII GGCC 2 cut(s) 639, 801
HapII CCGG 5 cut(s) 284, 311, 323, 370, 578
HhaI GCGC 2 cut(s) 277, 577
Hin1II CATG 6 cut(s) 36, 153, 175, 457, 529, 730
Hin6I GCGC 2 cut(s) 275, 575
HinP1I GCGC 2 cut(s) 275, 575
HincII GTYRAC 2 cut(s) 40, 232
HindII GTYRAC 2 cut(s) 40, 232
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 2 cut(s) 53, 208
HpaII CCGG 5 cut(s) 284, 311, 323, 370, 578
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 40, 232
Hpy188I TCNGA 3 cut(s) 329, 484, 707
Hpy188III TCNNGA 5 cut(s) 145, 164, 311, 477, 496
Hpy8I GTNNAC 2 cut(s) 40, 232
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 4 cut(s) 119, 234, 526, 550
HpyCH4III ACNGT 2 cut(s) 383, 819
HpyCH4V TGCA 3 cut(s) 24, 357, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 459, 546, 737
Hsp92II CATG 6 cut(s) 36, 153, 175, 457, 529, 730
HspAI GCGC 2 cut(s) 275, 575
Kpn2I TCCGGA 1 cut(s) 310
KroI GCCGGC 1 cut(s) 577
KroNI GCCGGC 1 cut(s) 579
Kzo9I GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
LguI GCTCTTC 1 cut(s) 650
LmnI GCTCC 1 cut(s) 450
LweI GCATC 2 cut(s) 344, 575
MaeIII GTNAC 3 cut(s) 152, 608, 711
MalI GATC 7 cut(s) 481, 486, 494, 500, 625, 647, 768
MboI GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
MboII GAAGA 3 cut(s) 62, 406, 667
MflI RGATCY 2 cut(s) 645, 766
MluCI AATT 6 cut(s) 70, 91, 265, 296, 420, 591
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 307
MreI CGCCGGCG 1 cut(s) 577
MroI TCCGGA 1 cut(s) 310
MroNI GCCGGC 1 cut(s) 577
MseI TTAA 4 cut(s) 83, 243, 758, 795
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 5 cut(s) 284, 311, 323, 370, 578
MspR9I CCNGG 2 cut(s) 438, 813
MvaI CCWGG 2 cut(s) 438, 813
MwoI GCNNNNNNNGC 3 cut(s) 459, 546, 737
NaeI GCCGGC 1 cut(s) 579
NcoI CCATGG 1 cut(s) 171
NdeII GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
NgoMIV GCCGGC 1 cut(s) 577
NlaIII CATG 6 cut(s) 36, 153, 175, 457, 529, 730
NspI RCATGY 1 cut(s) 153
PciI ACATGT 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 650
PdiI GCCGGC 1 cut(s) 579
PfeI GAWTC 1 cut(s) 208
Pfl23II CGTACG 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 811
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PscI ACATGT 1 cut(s) 149
PshBI ATTAAT 1 cut(s) 758
Psp6I CCWGG 2 cut(s) 436, 811
PspFI CCCAGC 2 cut(s) 97, 203
PspGI CCWGG 2 cut(s) 436, 811
PspLI CGTACG 1 cut(s) 378
PspPI GGNCC 1 cut(s) 320
PsuI RGATCY 2 cut(s) 645, 766
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SalI GTCGAC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 650
SaqAI TTAA 4 cut(s) 83, 243, 758, 795
Sau3AI GATC 7 cut(s) 479, 484, 492, 498, 623, 645, 766
Sau96I GGNCC 1 cut(s) 320
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 438, 813
SetI ASST 8 cut(s) 21, 103, 217, 305, 561, 574, 662, 697
SfaNI GCATC 2 cut(s) 344, 575
SgrAI CRCCGGYG 1 cut(s) 577
SinI GGWCC 1 cut(s) 320
Sse9I AATT 6 cut(s) 70, 91, 265, 296, 420, 591
SsiI CCGC 4 cut(s) 117, 341, 390, 553
StyD4I CCNGG 2 cut(s) 436, 811
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 383, 819
TaqI TCGA 3 cut(s) 231, 476, 522
TasI AATT 6 cut(s) 70, 91, 265, 296, 420, 591
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 4 cut(s) 83, 243, 758, 795
Tru9I TTAA 4 cut(s) 83, 243, 758, 795
TspDTI ATGAA 1 cut(s) 21
TspGWI ACGGA 2 cut(s) 364, 582
VpaK11BI GGWCC 1 cut(s) 320
VspI ATTAAT 1 cut(s) 758
XapI RAATTY 1 cut(s) 91
XceI RCATGY 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.