Rmu_sc0001385.1_g000023

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001385.1
OG0021628
Physical Location & Seq
Forward (+)
102693 .. 103412
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001385.1_g000023.1.cds

Sequence Viewer

Length: 720 bp
atgaatggggtagataaaagaataaggcattggtttatatcaaagatcgggtcaatagaaagattgagggatggaagcagcgctccttgtctttggctggaagagaagttcgtatcaaatcagttgctatggctattcctgcctatcctatggcctgcttcaatttctccagaaaacatttgtgataagataaactctactttggggaagttttggtggggagcagaccaagagggtcaaaaaattcactggaagagctggtcctaccttggtactcctaaaactgagggtggaatgggttttcaagatttaaatcatttcaatctggccctgcttgcaaagcaatgctggcgccttatacaagagcctaattctctatgggcacaagttatcaaggccaaatacttccctaactgcagtttcttgaaagctgtcaagggctatagggcatcctggagttggtctagcttactggaggctagggaccagtttattggggggggggggggcaactggcaagtccctcctccaaatggtggctttctagttacttctgggactccacaggtgggtaatcctacatgggttatctctttaatcgattgggatacttgtaattggaacctagactgcattttgcgtatcatacaacctagtaaagtgaggaaaattcttgctattcctattggcgatgaggaggggggtgacagattagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

239

Amino Acids

27.08

Weight (kDa)

8.73

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 536
AcsI RAATTY 2 cut(s) 243, 669
AcyI GRCGYC 1 cut(s) 352
AfaI GTAC 1 cut(s) 274
AfeI AGCGCT 1 cut(s) 82
AfiI CCNNNNNNNGG 4 cut(s) 459, 533, 536, 569
AgsI TTSAA 4 cut(s) 162, 305, 322, 427
AjnI CCWGG 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 3 cut(s) 258, 431, 468
AluI AGCT 3 cut(s) 258, 431, 468
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 3 cut(s) 152, 327, 396
ApeKI GCWGC 1 cut(s) 78
ApoI RAATTY 2 cut(s) 243, 669
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AspLEI GCGC 2 cut(s) 83, 354
AspS9I GGNCC 3 cut(s) 261, 328, 484
AsuHPI GGTGA 1 cut(s) 716
AvaII GGWCC 2 cut(s) 261, 484
BaeGI GKGCMC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 90
BccI CCATC 1 cut(s) 65
BciT130I CCWGG 1 cut(s) 454
BciVI GTATCC 1 cut(s) 601
BfaI CTAG 5 cut(s) 465, 480, 545, 626, 654
BfmI CTRYAG 2 cut(s) 415, 442
BfoI RGCGCY 2 cut(s) 84, 355
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 454
Bme18I GGWCC 2 cut(s) 261, 484
BmgT120I GGNCC 3 cut(s) 261, 328, 484
BmiI GGNNCC 3 cut(s) 353, 485, 623
BmrFI CCNGG 1 cut(s) 454
BmsI GCATC 1 cut(s) 458
BpmI CTGGAG 3 cut(s) 153, 475, 494
Bsa29I ATCGAT 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 352
BsaJI CCNNGG 1 cut(s) 268
Bsc4I CCNNNNNNNGG 4 cut(s) 459, 533, 536, 569
Bse1I ACTGG 4 cut(s) 254, 477, 487, 518
Bse3DI GCAATG 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 1 cut(s) 454
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 2 cut(s) 76, 449
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 4 cut(s) 459, 533, 536, 569
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 4 cut(s) 254, 477, 487, 518
BseRI GAGGAG 2 cut(s) 516, 710
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 1 cut(s) 90
BshFI GGCC 3 cut(s) 154, 329, 398
BshNI GGYRCC 1 cut(s) 351
BshVI ATCGAT 1 cut(s) 600
BslFI GGGAC 3 cut(s) 497, 506, 571
BslI CCNNNNNNNGG 4 cut(s) 459, 533, 536, 569
BsmFI GGGAC 3 cut(s) 497, 506, 571
BsnI GGCC 3 cut(s) 154, 329, 398
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 1 cut(s) 45
BspANI GGCC 3 cut(s) 154, 329, 398
BspCNI CTCAG 1 cut(s) 277
BspDI ATCGAT 1 cut(s) 600
BspLI GGNNCC 3 cut(s) 353, 485, 623
BspMAI CTGCAG 1 cut(s) 419
BspQI GCTCTTC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 4 cut(s) 254, 477, 487, 518
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 268
Bst2UI CCWGG 1 cut(s) 454
Bst6I CTCTTC 2 cut(s) 96, 248
BstACI GRCGYC 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 156, 336, 350
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 76, 449
BstH2I RGCGCY 2 cut(s) 84, 355
BstHHI GCGC 2 cut(s) 83, 354
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 139, 335, 340, 349
BstNI CCWGG 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 452
BstSFI CTRYAG 2 cut(s) 415, 442
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 90
BstXI CCANNNNNNTGG 1 cut(s) 494
Bsu15I ATCGAT 1 cut(s) 600
BsuI GTATCC 1 cut(s) 601
BsuRI GGCC 3 cut(s) 154, 329, 398
BsuTUI ATCGAT 1 cut(s) 600
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 2 cut(s) 76, 449
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 156, 336, 350
CfoI GCGC 2 cut(s) 83, 354
Cfr13I GGNCC 3 cut(s) 261, 328, 484
ClaI ATCGAT 1 cut(s) 600
Csp6I GTAC 1 cut(s) 273
CviAII CATG 1 cut(s) 582
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 1 cut(s) 285
DinI GGCGCC 1 cut(s) 353
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
DraI TTTAAA 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 96, 248
EarI CTCTTC 2 cut(s) 96, 248
Eco130I CCWWGG 1 cut(s) 268
Eco47I GGWCC 2 cut(s) 261, 484
Eco47III AGCGCT 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 452
EcoT14I CCWWGG 1 cut(s) 268
EgeI GGCGCC 1 cut(s) 353
EheI GGCGCC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 1 cut(s) 585
FaiI YATR 8 cut(s) 38, 130, 151, 359, 379, 444, 583, 647
FaqI GGGAC 3 cut(s) 497, 506, 571
FatI CATG 1 cut(s) 581
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 2 cut(s) 83, 436
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 5 cut(s) 465, 480, 545, 626, 654
GlaI GCGC 2 cut(s) 82, 353
GluI GCNGC 1 cut(s) 79
GsuI CTGGAG 3 cut(s) 153, 475, 494
HaeII RGCGCY 2 cut(s) 84, 355
HaeIII GGCC 3 cut(s) 154, 329, 398
HhaI GCGC 2 cut(s) 83, 354
Hin1I GRCGYC 1 cut(s) 352
Hin1II CATG 1 cut(s) 585
Hin6I GCGC 2 cut(s) 81, 352
HinP1I GCGC 2 cut(s) 81, 352
HinfI GANTC 1 cut(s) 559
HphI GGTGA 1 cut(s) 716
Hpy188III TCNNGA 3 cut(s) 170, 305, 424
HpyCH4V TGCA 3 cut(s) 338, 417, 633
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 335, 340, 349
HpyF3I CTNAG 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 352
Hsp92II CATG 1 cut(s) 585
HspAI GCGC 2 cut(s) 81, 352
KasI GGCGCC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 45
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 2 cut(s) 88, 221
Lsp1109I GCAGC 1 cut(s) 90
LweI GCATC 1 cut(s) 458
MaeI CTAG 5 cut(s) 465, 480, 545, 626, 654
MaeIII GTNAC 2 cut(s) 547, 704
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MboII GAAGA 2 cut(s) 113, 265
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 5 cut(s) 162, 243, 370, 616, 669
Mly113I GGCGCC 1 cut(s) 352
MlyI GAGTC 1 cut(s) 553
MnlI CCTC 9 cut(s) 60, 226, 280, 469, 534, 537, 657, 688, 691
MseI TTAA 2 cut(s) 311, 596
MspR9I CCNGG 1 cut(s) 454
MvaI CCWGG 1 cut(s) 454
MwoI GCNNNNNNNGC 4 cut(s) 139, 335, 340, 349
NarI GGCGCC 1 cut(s) 352
NdeII GATC 1 cut(s) 45
NlaIII CATG 1 cut(s) 585
NlaIV GGNNCC 3 cut(s) 353, 485, 623
NmuCI GTSAC 1 cut(s) 704
PciSI GCTCTTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 452
PkrI GCNGC 1 cut(s) 80
PleI GAGTC 1 cut(s) 553
PluTI GGCGCC 1 cut(s) 355
PpsI GAGTC 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 353, 485, 623
PspPI GGNCC 3 cut(s) 261, 328, 484
PstI CTGCAG 1 cut(s) 419
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 311, 596
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 3 cut(s) 261, 328, 484
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 1 cut(s) 454
SduI GDGCHC 1 cut(s) 385
SetI ASST 7 cut(s) 260, 270, 433, 470, 570, 627, 655
SfaNI GCATC 1 cut(s) 458
SfcI CTRYAG 2 cut(s) 415, 442
SfoI GGCGCC 1 cut(s) 353
SinI GGWCC 2 cut(s) 261, 484
SmiI ATTTAAAT 1 cut(s) 312
Sse9I AATT 5 cut(s) 162, 243, 370, 616, 669
SspDI GGCGCC 1 cut(s) 351
SspMI CTAG 5 cut(s) 465, 480, 545, 626, 654
StyD4I CCNGG 1 cut(s) 452
StyI CCWWGG 1 cut(s) 268
SwaI ATTTAAAT 1 cut(s) 312
TaqI TCGA 1 cut(s) 600
TasI AATT 5 cut(s) 162, 243, 370, 616, 669
Tru1I TTAA 2 cut(s) 311, 596
Tru9I TTAA 2 cut(s) 311, 596
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 1 cut(s) 704
TseI GCWGC 1 cut(s) 78
Tsp45I GTSAC 1 cut(s) 704
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 254
Van91I CCANNNNNTGG 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 261, 484
XapI RAATTY 2 cut(s) 243, 669
XspI CTAG 5 cut(s) 465, 480, 545, 626, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.