Rmu_sc0037484.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0037484.1
OG0021628
Physical Location & Seq
Forward (+)
15 .. 1742
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0037484.1_g000001.1.cds

Sequence Viewer

Length: 819 bp
atggcatgcttcaaattccccaatggaatttgtgatgagattaactccgctttagggaagttttggtggggatctgatcaggagcatcaaaagatccactggaagagttggggttatttgggattacccaaggcggagggaggtttgggttttagggacctccagaactttaatctggccttcctggccaagcaatgttggagattaatacatgagcctacatctccttgggcacaagttctcaaggccaggtactttccgaattgtaatttcttgtctgctgagaaaggctatcgagcctcgtgggatatttggaaagacaagtggatccctcctccaaatgggggttgtttggcaacaaatggtctgccccataccaatgctccaggagctcccagaaggcagtgtgcaaaagtgaattgggacgcggcatggactgcacctaactccgccgggttgtgtgtgatgatacgtgatagtacaggctctgttttgggtggttttgcttgccgggcttcttgtgcctcagttcccattgcagaagctgaagcgattttggcaggggtcgagcttgctatctccatgaatctgaaaattatcatatttgaatcagactccaaagaagttgtcactgatttgaagggcaatactgcaagtaggaattggcaaacctttccaatcattaatctgattaggaatcgttgccctttctttgatgggacggtgggtcctttgagatgggcttctatgccaccaccatccttgactcatgttctgagaaatgatggactgccatgcccgcctgcggaggccacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

272

Amino Acids

29.98

Weight (kDa)

8.64

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 428
AciI CCGC 6 cut(s) 48, 134, 428, 450, 800, 806
AclWI GGATC 4 cut(s) 79, 88, 322, 335
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 14, 27
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 2 cut(s) 254, 481
AfiI CCNNNNNNNGG 4 cut(s) 54, 341, 344, 805
AgsI TTSAA 3 cut(s) 13, 608, 640
AhdI GACNNNNNGTC 1 cut(s) 726
AjnI CCWGG 3 cut(s) 183, 248, 385
AluBI AGCT 3 cut(s) 392, 545, 571
AluI AGCT 3 cut(s) 392, 545, 571
Alw21I GWGCWC 1 cut(s) 394
AlwI GGATC 4 cut(s) 79, 88, 322, 335
AlwNI CAGNNNCTG 2 cut(s) 488, 545
AoxI GGCC 4 cut(s) 177, 186, 246, 810
ApoI RAATTY 2 cut(s) 14, 27
ArsI GACNNNNNNTTYG 2 cut(s) 612, 644
AseI ATTAAT 2 cut(s) 206, 684
AspS9I GGNCC 2 cut(s) 157, 728
AsuC2I CCSGG 2 cut(s) 454, 512
AvaII GGWCC 2 cut(s) 157, 728
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 188
BamHI GGATCC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 394
BauI CACGAG 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 394
BccI CCATC 4 cut(s) 710, 732, 766, 779
BciT130I CCWGG 3 cut(s) 185, 250, 387
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 2 cut(s) 454, 512
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 5 cut(s) 185, 250, 387, 454, 512
Bme18I GGWCC 2 cut(s) 157, 728
BmeRI GACNNNNNGTC 1 cut(s) 726
BmgT120I GGNCC 2 cut(s) 157, 728
BmiI GGNNCC 3 cut(s) 158, 329, 729
BmrFI CCNGG 5 cut(s) 185, 250, 387, 454, 512
BmsI GCATC 1 cut(s) 94
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 2 cut(s) 146, 369
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 454, 512
BsaAI YACGTR 1 cut(s) 473
BsaJI CCNNGG 2 cut(s) 129, 227
BsaXI ACNNNNNCTCC 2 cut(s) 367, 397
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 341, 344, 805
Bse1I ACTGG 1 cut(s) 104
Bse3DI GCAATG 2 cut(s) 200, 534
BseBI CCWGG 3 cut(s) 185, 250, 387
BseDI CCNNGG 2 cut(s) 129, 227
BseGI GGATG 1 cut(s) 758
BseLI CCNNNNNNNGG 4 cut(s) 54, 341, 344, 805
BseMI GCAATG 2 cut(s) 200, 534
BseMII CTCAG 3 cut(s) 273, 540, 767
BseNI ACTGG 1 cut(s) 104
BseRI GAGGAG 1 cut(s) 324
BseSI GKGCMC 1 cut(s) 235
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 4 cut(s) 179, 188, 248, 812
BsiHKAI GWGCWC 1 cut(s) 394
BsiSI CCGG 2 cut(s) 453, 511
BslFI GGGAC 3 cut(s) 170, 437, 733
BslI CCNNNNNNNGG 4 cut(s) 54, 341, 344, 805
BsmFI GGGAC 3 cut(s) 170, 437, 733
BsnI GGCC 4 cut(s) 179, 188, 248, 812
Bsp1286I GDGCHC 2 cut(s) 235, 394
Bsp143I GATC 4 cut(s) 71, 76, 93, 327
BspACI CCGC 6 cut(s) 48, 134, 428, 450, 800, 806
BspANI GGCC 4 cut(s) 179, 188, 248, 812
BspCNI CTCAG 3 cut(s) 274, 539, 768
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 3 cut(s) 158, 329, 729
BspPI GGATC 4 cut(s) 79, 88, 322, 335
BsrDI GCAATG 2 cut(s) 200, 534
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 129, 227
BssMI GATC 4 cut(s) 71, 76, 93, 327
BssSI CACGAG 1 cut(s) 301
BssT1I CCWWGG 2 cut(s) 129, 227
Bst2BI CACGAG 1 cut(s) 301
Bst2UI CCWGG 3 cut(s) 185, 250, 387
Bst4CI ACNGT 1 cut(s) 724
Bst6I CTCTTC 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 437
BstBAI YACGTR 1 cut(s) 473
BstC8I GCNNGC 5 cut(s) 7, 508, 573, 800, 804
BstDEI CTNAG 3 cut(s) 282, 526, 776
BstF5I GGATG 1 cut(s) 758
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 4 cut(s) 74, 79, 96, 330
BstMBI GATC 4 cut(s) 71, 76, 93, 327
BstMWI GCNNNNNNNGC 7 cut(s) 185, 389, 437, 512, 521, 557, 799
BstNI CCWGG 3 cut(s) 185, 250, 387
BstNSI RCATGY 1 cut(s) 9
BstSCI CCNGG 5 cut(s) 183, 248, 385, 452, 510
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 428
BstX2I RGATCY 3 cut(s) 71, 93, 327
BstYI RGATCY 3 cut(s) 71, 93, 327
BsuRI GGCC 4 cut(s) 179, 188, 248, 812
BtsCI GGATG 1 cut(s) 758
BtsI GCAGTG 1 cut(s) 410
BtsIMutI CAGTG 3 cut(s) 97, 410, 630
Cac8I GCNNGC 5 cut(s) 7, 508, 573, 800, 804
CaiI CAGNNNCTG 2 cut(s) 488, 545
Cfr13I GGNCC 2 cut(s) 157, 728
CseI GACGC 1 cut(s) 434
Csp6I GTAC 2 cut(s) 253, 480
CspCI CAANNNNNGTGG 2 cut(s) 744, 779
CviAII CATG 6 cut(s) 6, 212, 432, 583, 770, 795
CviQI GTAC 2 cut(s) 253, 480
DdeI CTNAG 3 cut(s) 282, 526, 776
DpnI GATC 4 cut(s) 73, 78, 95, 329
DpnII GATC 4 cut(s) 71, 76, 93, 327
DriI GACNNNNNGTC 1 cut(s) 726
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 98
Eam1105I GACNNNNNGTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 98
EciI GGCGGA 2 cut(s) 149, 439
Ecl136II GAGCTC 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 129, 227
Eco24I GRGCYC 1 cut(s) 394
Eco47I GGWCC 2 cut(s) 157, 728
Eco53kI GAGCTC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 392
EcoO109I RGGNCCY 2 cut(s) 157, 728
EcoRII CCWGG 3 cut(s) 183, 248, 385
EcoT14I CCWWGG 2 cut(s) 129, 227
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 2 cut(s) 129, 227
FaeI CATG 6 cut(s) 9, 215, 435, 586, 773, 798
FaiI YATR 9 cut(s) 7, 213, 375, 433, 584, 602, 749, 771, 796
FaqI GGGAC 3 cut(s) 170, 437, 733
FatI CATG 6 cut(s) 5, 211, 431, 582, 769, 794
FauI CCCGC 1 cut(s) 807
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 1 cut(s) 745
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 1 cut(s) 429
GluI GCNGC 1 cut(s) 429
GsuI CTGGAG 2 cut(s) 146, 369
HaeIII GGCC 4 cut(s) 179, 188, 248, 812
HapII CCGG 2 cut(s) 453, 511
HgaI GACGC 1 cut(s) 434
Hin1II CATG 6 cut(s) 9, 215, 435, 586, 773, 798
HinfI GANTC 5 cut(s) 586, 608, 614, 697, 766
HpaII CCGG 2 cut(s) 453, 511
Hpy188I TCNGA 6 cut(s) 76, 261, 591, 613, 690, 777
Hpy188III TCNNGA 2 cut(s) 80, 163
HpyAV CCTTC 3 cut(s) 190, 393, 634
HpyCH4III ACNGT 1 cut(s) 724
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 4 cut(s) 410, 440, 539, 653
HpyF10VI GCNNNNNNNGC 7 cut(s) 185, 389, 437, 512, 521, 557, 799
HpyF3I CTNAG 3 cut(s) 282, 526, 776
HpySE526I ACGT 1 cut(s) 472
Hsp92II CATG 6 cut(s) 9, 215, 435, 586, 773, 798
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 71, 76, 93, 327
LmnI GCTCC 4 cut(s) 82, 388, 389, 397
LweI GCATC 1 cut(s) 94
MaeII ACGT 1 cut(s) 472
MaeIII GTNAC 1 cut(s) 628
MalI GATC 4 cut(s) 73, 78, 95, 329
MboI GATC 4 cut(s) 71, 76, 93, 327
MboII GAAGA 1 cut(s) 115
MflI RGATCY 3 cut(s) 71, 93, 327
MhlI GDGCHC 2 cut(s) 235, 394
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 7 cut(s) 14, 27, 262, 268, 418, 594, 661
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 608, 760
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 8 cut(s) 130, 134, 170, 310, 342, 345, 535, 802
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 4 cut(s) 42, 171, 206, 684
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 188
MspI CCGG 2 cut(s) 453, 511
MspR9I CCNGG 5 cut(s) 185, 250, 387, 454, 512
MvaI CCWGG 3 cut(s) 185, 250, 387
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 7 cut(s) 185, 389, 437, 512, 521, 557, 799
NciI CCSGG 2 cut(s) 454, 512
NdeII GATC 4 cut(s) 71, 76, 93, 327
NlaIII CATG 6 cut(s) 9, 215, 435, 586, 773, 798
NlaIV GGNNCC 3 cut(s) 158, 329, 729
NmuCI GTSAC 1 cut(s) 628
NspI RCATGY 1 cut(s) 9
PaeI GCATGC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 586, 608, 697
PfoI TCCNGGA 1 cut(s) 385
PkrI GCNGC 1 cut(s) 430
PleI GAGTC 2 cut(s) 608, 760
PpsI GAGTC 2 cut(s) 608, 760
Ppu21I YACGTR 1 cut(s) 473
PpuMI RGGWCCY 2 cut(s) 157, 728
PshBI ATTAAT 2 cut(s) 206, 684
Psp124BI GAGCTC 1 cut(s) 394
Psp5II RGGWCCY 2 cut(s) 157, 728
Psp6I CCWGG 3 cut(s) 183, 248, 385
PspGI CCWGG 3 cut(s) 183, 248, 385
PspN4I GGNNCC 3 cut(s) 158, 329, 729
PspPI GGNCC 2 cut(s) 157, 728
PspPPI RGGWCCY 2 cut(s) 157, 728
PstNI CAGNNNCTG 2 cut(s) 488, 545
PsuI RGATCY 3 cut(s) 71, 93, 327
RsaI GTAC 2 cut(s) 254, 481
RsaNI GTAC 2 cut(s) 253, 480
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 394
SaqAI TTAA 4 cut(s) 42, 171, 206, 684
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 4 cut(s) 71, 76, 93, 327
Sau96I GGNCC 2 cut(s) 157, 728
SchI GAGTC 2 cut(s) 608, 760
ScrFI CCNGG 5 cut(s) 185, 250, 387, 454, 512
SduI GDGCHC 2 cut(s) 235, 394
SetI ASST 9 cut(s) 145, 162, 254, 394, 445, 475, 547, 573, 674
SfaNI GCATC 1 cut(s) 94
SfiI GGCCNNNNNGGCC 1 cut(s) 185
SinI GGWCC 2 cut(s) 157, 728
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SphI GCATGC 1 cut(s) 9
Sse9I AATT 7 cut(s) 14, 27, 262, 268, 418, 594, 661
SsiI CCGC 6 cut(s) 48, 134, 428, 450, 800, 806
SstI GAGCTC 1 cut(s) 394
StyD4I CCNGG 5 cut(s) 183, 248, 385, 452, 510
StyI CCWWGG 2 cut(s) 129, 227
TaaI ACNGT 1 cut(s) 724
TaiI ACGT 1 cut(s) 475
TaqI TCGA 2 cut(s) 295, 567
TasI AATT 7 cut(s) 14, 27, 262, 268, 418, 594, 661
TatI WGTACW 1 cut(s) 479
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 3 cut(s) 586, 608, 697
Tru1I TTAA 4 cut(s) 42, 171, 206, 684
Tru9I TTAA 4 cut(s) 42, 171, 206, 684
TscAI CASTG 3 cut(s) 104, 410, 637
TseFI GTSAC 1 cut(s) 628
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 1 cut(s) 599
TspRI CASTG 3 cut(s) 104, 410, 637
VpaK11BI GGWCC 2 cut(s) 157, 728
VspI ATTAAT 2 cut(s) 206, 684
XapI RAATTY 2 cut(s) 14, 27
XceI RCATGY 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.