Rmu_sc0003543.1_g000010

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003543.1
OG0021628
Physical Location & Seq
Forward (+)
29374 .. 29829
456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003543.1_g000010.1.cds

Sequence Viewer

Length: 456 bp
atgaatggggtagataaaagaataaggcattggtttatatcaaagatcgggtcaatagaaagattgagggatggaagcagcgctccttgtctttggctggaagagaagttcgtatcaaatcagttgctatggctattcctgcctatcctatggcctgcttcaatttctctagaaaacatttgtgataagataaactctactttggggaagttttggtggggagcagaccaagagggtcaaaaaattcactggaagagctggtcctaccttggtactcctaaaactgagggtggaatgggttttcaagatttaaatcatttcaatctggccctgcttgcaaagcaatgctggcgccttatacaagagcctaattctctatgggcacaagttatcaaggccagatacttccctaactgcagtttcttgaaagctgtcaagggctatagggcatcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

151

Amino Acids

17.52

Weight (kDa)

9.42

Isoelectric Point (pI)

35.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AcsI RAATTY 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 352
AfaI GTAC 1 cut(s) 274
AfeI AGCGCT 1 cut(s) 82
AgsI TTSAA 4 cut(s) 162, 305, 322, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AloI GAACNNNNNNTCC 2 cut(s) 92, 124
AluBI AGCT 2 cut(s) 258, 431
AluI AGCT 2 cut(s) 258, 431
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 3 cut(s) 152, 327, 396
ApeKI GCWGC 1 cut(s) 78
ApoI RAATTY 1 cut(s) 243
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AspLEI GCGC 2 cut(s) 83, 354
AspS9I GGNCC 2 cut(s) 261, 328
AvaII GGWCC 1 cut(s) 261
BaeGI GKGCMC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 351
BbvI GCAGC 1 cut(s) 90
BccI CCATC 1 cut(s) 65
BfaI CTAG 2 cut(s) 170, 454
BfmI CTRYAG 2 cut(s) 415, 442
BfoI RGCGCY 2 cut(s) 84, 355
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 261, 328
BmiI GGNNCC 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 352
BsaJI CCNNGG 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 2 cut(s) 76, 449
BseJI GATNNNNATC 1 cut(s) 312
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 1 cut(s) 90
BshFI GGCC 3 cut(s) 154, 329, 398
BshNI GGYRCC 1 cut(s) 351
BsnI GGCC 3 cut(s) 154, 329, 398
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 1 cut(s) 45
BspANI GGCC 3 cut(s) 154, 329, 398
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 353
BspMAI CTGCAG 1 cut(s) 419
BspQI GCTCTTC 1 cut(s) 248
BspT107I GGYRCC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 1 cut(s) 45
BssNI GRCGYC 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 268
Bst6I CTCTTC 2 cut(s) 96, 248
BstACI GRCGYC 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 156, 336, 350
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 76, 449
BstH2I RGCGCY 2 cut(s) 84, 355
BstHHI GCGC 2 cut(s) 83, 354
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 139, 335, 340, 349
BstSFI CTRYAG 2 cut(s) 415, 442
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 90
BsuRI GGCC 3 cut(s) 154, 329, 398
BtsCI GGATG 2 cut(s) 76, 449
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 156, 336, 350
CfoI GCGC 2 cut(s) 83, 354
Cfr13I GGNCC 2 cut(s) 261, 328
Csp6I GTAC 1 cut(s) 273
CviJI RGCY 9 cut(s) 97, 133, 154, 258, 329, 367, 398, 431, 441
CviKI_1 RGCY 9 cut(s) 97, 133, 154, 258, 329, 367, 398, 431, 441
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 1 cut(s) 285
DinI GGCGCC 1 cut(s) 353
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
DraI TTTAAA 1 cut(s) 312
Eam1104I CTCTTC 2 cut(s) 96, 248
EarI CTCTTC 2 cut(s) 96, 248
Eco130I CCWWGG 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 261
Eco47III AGCGCT 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 268
EgeI GGCGCC 1 cut(s) 353
EheI GGCGCC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 268
FaiI YATR 6 cut(s) 38, 130, 151, 359, 379, 444
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 2 cut(s) 83, 436
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 2 cut(s) 170, 454
GlaI GCGC 2 cut(s) 82, 353
GluI GCNGC 1 cut(s) 79
HaeII RGCGCY 2 cut(s) 84, 355
HaeIII GGCC 3 cut(s) 154, 329, 398
HhaI GCGC 2 cut(s) 83, 354
Hin1I GRCGYC 1 cut(s) 352
Hin6I GCGC 2 cut(s) 81, 352
HinP1I GCGC 2 cut(s) 81, 352
Hpy188III TCNNGA 3 cut(s) 170, 305, 424
HpyCH4V TGCA 2 cut(s) 338, 417
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 335, 340, 349
HpyF3I CTNAG 1 cut(s) 285
Hsp92I GRCGYC 1 cut(s) 352
HspAI GCGC 2 cut(s) 81, 352
KasI GGCGCC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 45
LguI GCTCTTC 1 cut(s) 248
LmnI GCTCC 2 cut(s) 88, 221
LpnPI CCDG 9 cut(s) 83, 152, 168, 235, 244, 311, 334, 344, 412
Lsp1109I GCAGC 1 cut(s) 90
MaeI CTAG 2 cut(s) 170, 454
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MboII GAAGA 2 cut(s) 113, 265
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 3 cut(s) 162, 243, 370
Mly113I GGCGCC 1 cut(s) 352
MnlI CCTC 3 cut(s) 60, 226, 280
MseI TTAA 1 cut(s) 311
MwoI GCNNNNNNNGC 4 cut(s) 139, 335, 340, 349
NarI GGCGCC 1 cut(s) 352
NdeII GATC 1 cut(s) 45
NlaIV GGNNCC 1 cut(s) 353
PciSI GCTCTTC 1 cut(s) 248
PkrI GCNGC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 2 cut(s) 261, 328
PstI CTGCAG 1 cut(s) 419
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 248
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 2 cut(s) 261, 328
SduI GDGCHC 1 cut(s) 385
SetI ASST 3 cut(s) 260, 270, 433
SfcI CTRYAG 2 cut(s) 415, 442
SfoI GGCGCC 1 cut(s) 353
SinI GGWCC 1 cut(s) 261
SmiI ATTTAAAT 1 cut(s) 312
Sse9I AATT 3 cut(s) 162, 243, 370
SspDI GGCGCC 1 cut(s) 351
SspMI CTAG 2 cut(s) 170, 454
StyI CCWWGG 1 cut(s) 268
SwaI ATTTAAAT 1 cut(s) 312
TasI AATT 3 cut(s) 162, 243, 370
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 1 cut(s) 254
TseI GCWGC 1 cut(s) 78
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 261
XapI RAATTY 1 cut(s) 243
XbaI TCTAGA 1 cut(s) 169
XspI CTAG 2 cut(s) 170, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.