Rmu_sc0000539.1_g000071

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000539.1
OG0018055
Physical Location & Seq
Reverse (-)
314250 .. 314996
747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000539.1_g000071.1.cds

Sequence Viewer

Length: 747 bp
atgaactaccctcagcaaccctacccgggggtcccgccgcagggctacccgggctacggctataacccttacccaccagccgcatacgcccaagacccagccgccgcctattacgccacgcagacatattcaccggcagaggaaggggccaaccactgcttccgctgcatctgcgtaatcatgctggtatcactcatgtttaccctcattgcaagcatcatcctcctcttcgtcgccaaacccgagcatgcctccttcaaggtagattccctcagcgtctccaacttcaacacaaaccctgcaaacaaagtcctcacgggaaaatgggaggccaaagtgaatgtggaaaacggtaggacgagactcaatatcgagaacgtggagcccagtctgtaccaaaaggacacctggttggcgcgcgggtcgtccgtgggcatatcgtcctccgggtattgggacgagtacaattgggggagttctcctagcacgatggagttcaaattcgaggccaacaatgtctcggcgccggcggcggatattgaaaaggagatcaaagaaacgggaggcgtgacgttcaatttgagcatagatatgaaatatgtgtcgaggtcttctgggttttggtggcatgtgaagagtgggagcatgaaagttacgtgcaaagatttaaagattggtttatcggtcggtgctaatagtggatcgcttactggtgaaaaaaaaaattgtgaagtttccggtggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

248

Amino Acids

27.09

Weight (kDa)

6.32

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018055)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20200
malus_domestica MD14G1037700.v1.1
prunus_persica Prupe.7G096900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475721
rosa_laevigata RLG00000023821
rosa_multiflora Rmu_sc0000539.1_g000071
rosa_roxburghii Rroxscaffold_6G00405910
rosa_samantha Rh3BG231200 Rh3CG225800 Rh3DG226300
rosa_wichuraiana Rw3G018260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 523
AccII CGCG 2 cut(s) 418, 420
AciI CCGC 9 cut(s) 35, 38, 81, 102, 105, 163, 420, 530, 533
AclWI GGATC 1 cut(s) 709
AcsI RAATTY 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 2 cut(s) 395, 464
AfiI CCNNNNNNNGG 6 cut(s) 26, 27, 40, 41, 56, 453
AgsI TTSAA 5 cut(s) 259, 289, 499, 542, 577
AjnI CCWGG 1 cut(s) 407
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
Alw26I GTCTC 3 cut(s) 283, 355, 523
AlwI GGATC 1 cut(s) 709
Ama87I CYCGRG 3 cut(s) 25, 49, 242
AoxI GGCC 3 cut(s) 147, 330, 507
ApeKI GCWGC 1 cut(s) 165
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AspLEI GCGC 3 cut(s) 418, 420, 526
AspS9I GGNCC 2 cut(s) 31, 147
AsuC2I CCSGG 5 cut(s) 26, 27, 50, 51, 448
AsuHPI GGTGA 2 cut(s) 123, 725
AvaI CYCGRG 3 cut(s) 25, 49, 242
AvaII GGWCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 1 cut(s) 387
BbsI GAAGAC 1 cut(s) 603
BbvCI CCTCAGC 2 cut(s) 12, 272
BbvI GCAGC 1 cut(s) 152
BccI CCATC 1 cut(s) 484
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 409
BcnI CCSGG 5 cut(s) 26, 27, 50, 51, 448
BcoDI GTCTC 3 cut(s) 283, 355, 523
BfaI CTAG 1 cut(s) 483
BfoI RGCGCY 1 cut(s) 527
BisI GCNGC 6 cut(s) 38, 81, 102, 105, 166, 531
BlsI GCNGC 6 cut(s) 39, 82, 103, 106, 167, 532
Bme1390I CCNGG 6 cut(s) 26, 27, 50, 51, 409, 448
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 3 cut(s) 25, 49, 242
BmgT120I GGNCC 2 cut(s) 31, 147
BmiI GGNNCC 5 cut(s) 32, 33, 148, 384, 525
BmrFI CCNGG 6 cut(s) 26, 27, 50, 51, 409, 448
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 2 cut(s) 177, 225
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 603
BplI GAGNNNNNCTC 2 cut(s) 236, 268
Bpu10I CCTNAGC 2 cut(s) 12, 272
BpuMI CCSGG 5 cut(s) 26, 27, 50, 51, 448
BsaAI YACGTR 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 524
BsaJI CCNNGG 4 cut(s) 25, 26, 49, 429
BsaWI WCCGGW 1 cut(s) 737
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 27, 40, 41, 56, 453
Bse118I RCCGGY 2 cut(s) 133, 526
Bse1I ACTGG 2 cut(s) 387, 715
Bse3DI GCAATG 1 cut(s) 207
BseBI CCWGG 1 cut(s) 409
BseDI CCNNGG 4 cut(s) 25, 26, 49, 429
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 6 cut(s) 26, 27, 40, 41, 56, 453
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 26, 286
BseNI ACTGG 2 cut(s) 387, 715
BsePI GCGCGC 1 cut(s) 416
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 2 cut(s) 418, 420
Bsh1285I CGRYCG 1 cut(s) 687
BshFI GGCC 3 cut(s) 149, 332, 509
BshNI GGYRCC 1 cut(s) 523
BsiEI CGRYCG 1 cut(s) 687
BsiHKCI CYCGRG 3 cut(s) 25, 49, 242
BsiSI CCGG 6 cut(s) 26, 50, 134, 447, 527, 738
BslFI GGGAC 2 cut(s) 17, 470
BslI CCNNNNNNNGG 6 cut(s) 26, 27, 40, 41, 56, 453
BsmAI GTCTC 3 cut(s) 283, 355, 523
BsmBI CGTCTC 1 cut(s) 283
BsmFI GGGAC 2 cut(s) 17, 470
BsnI GGCC 3 cut(s) 149, 332, 509
BsoBI CYCGRG 3 cut(s) 25, 49, 242
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 549, 701
BspACI CCGC 9 cut(s) 35, 38, 81, 102, 105, 163, 420, 530, 533
BspANI GGCC 3 cut(s) 149, 332, 509
BspCNI CTCAG 2 cut(s) 25, 285
BspFNI CGCG 2 cut(s) 418, 420
BspLI GGNNCC 5 cut(s) 32, 33, 148, 384, 525
BspPI GGATC 1 cut(s) 709
BspT107I GGYRCC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 207
BsrFI RCCGGY 2 cut(s) 133, 526
BsrI ACTGG 2 cut(s) 387, 715
BssAI RCCGGY 2 cut(s) 133, 526
BssECI CCNNGG 4 cut(s) 25, 26, 49, 429
BssHII GCGCGC 1 cut(s) 416
BssMI GATC 2 cut(s) 549, 701
BssNI GRCGYC 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 409
Bst4CI ACNGT 1 cut(s) 353
Bst6I CTCTTC 2 cut(s) 233, 629
BstACI GRCGYC 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 657
BstC8I GCNNGC 4 cut(s) 214, 249, 418, 528
BstDEI CTNAG 2 cut(s) 12, 272
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 2 cut(s) 418, 420
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 3 cut(s) 418, 420, 526
BstKTI GATC 2 cut(s) 552, 704
BstMAI GTCTC 3 cut(s) 283, 355, 523
BstMBI GATC 2 cut(s) 549, 701
BstMCI CGRYCG 1 cut(s) 687
BstMWI GCNNNNNNNGC 6 cut(s) 51, 86, 113, 165, 171, 530
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 2 cut(s) 251, 632
BstSCI CCNGG 6 cut(s) 24, 25, 48, 49, 407, 446
BstUI CGCG 2 cut(s) 418, 420
BstV1I GCAGC 1 cut(s) 152
BstV2I GAAGAC 1 cut(s) 603
BsuRI GGCC 3 cut(s) 149, 332, 509
BtgI CCRYGG 1 cut(s) 429
BtsCI GGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 4 cut(s) 214, 249, 418, 528
CfoI GCGC 3 cut(s) 418, 420, 526
Cfr10I RCCGGY 2 cut(s) 133, 526
Cfr13I GGNCC 2 cut(s) 31, 147
Cfr9I CCCGGG 2 cut(s) 25, 49
CseI GACGC 1 cut(s) 265
CsiI ACCWGGT 1 cut(s) 407
Csp6I GTAC 2 cut(s) 394, 463
CviAII CATG 5 cut(s) 181, 196, 248, 629, 646
CviQI GTAC 2 cut(s) 394, 463
DdeI CTNAG 2 cut(s) 12, 272
DinI GGCGCC 1 cut(s) 525
DpnI GATC 2 cut(s) 551, 703
DpnII GATC 2 cut(s) 549, 701
DraI TTTAAA 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 233, 629
EarI CTCTTC 2 cut(s) 233, 629
EciI GGCGGA 1 cut(s) 548
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 31
Eco88I CYCGRG 3 cut(s) 25, 49, 242
EcoO109I RGGNCCY 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 407
EcoT38I GRGCYC 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 525
EheI GGCGCC 1 cut(s) 525
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 5 cut(s) 184, 199, 251, 632, 649
FaqI GGGAC 2 cut(s) 17, 470
FatI CATG 5 cut(s) 180, 195, 247, 628, 645
FauI CCCGC 2 cut(s) 42, 413
Fnu4HI GCNGC 6 cut(s) 38, 81, 102, 105, 166, 531
FokI GGATG 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 6 cut(s) 38, 81, 102, 105, 166, 531
FspBI CTAG 1 cut(s) 483
GlaI GCGC 3 cut(s) 417, 419, 525
GluI GCNGC 6 cut(s) 38, 81, 102, 105, 166, 531
GsaI CCCAGC 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 3 cut(s) 149, 332, 509
HapII CCGG 6 cut(s) 26, 50, 134, 447, 527, 738
HgaI GACGC 1 cut(s) 265
HhaI GCGC 3 cut(s) 418, 420, 526
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 5 cut(s) 184, 199, 251, 632, 649
Hin6I GCGC 3 cut(s) 416, 418, 524
HinP1I GCGC 3 cut(s) 416, 418, 524
HinfI GANTC 2 cut(s) 266, 363
HpaII CCGG 6 cut(s) 26, 50, 134, 447, 527, 738
HphI GGTGA 2 cut(s) 123, 725
Hpy166II GTNNAC 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 1 cut(s) 201
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 137, 265
HpyCH4III ACNGT 1 cut(s) 353
HpyCH4IV ACGT 3 cut(s) 378, 572, 656
HpyCH4V TGCA 4 cut(s) 168, 212, 302, 660
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 86, 113, 165, 171, 530
HpyF3I CTNAG 2 cut(s) 12, 272
HpySE526I ACGT 3 cut(s) 378, 572, 656
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 5 cut(s) 184, 199, 251, 632, 649
HspAI GCGC 3 cut(s) 416, 418, 524
KasI GGCGCC 1 cut(s) 523
KflI GGGWCCC 1 cut(s) 31
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
Kzo9I GATC 2 cut(s) 549, 701
LmnI GCTCC 2 cut(s) 382, 642
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 2 cut(s) 177, 225
MabI ACCWGGT 1 cut(s) 407
MaeI CTAG 1 cut(s) 483
MaeII ACGT 3 cut(s) 378, 572, 656
MaeIII GTNAC 2 cut(s) 568, 652
MalI GATC 2 cut(s) 551, 703
MboI GATC 2 cut(s) 549, 701
MboII GAAGA 3 cut(s) 220, 603, 646
MfeI CAATTG 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 4 cut(s) 466, 500, 577, 724
Mly113I GGCGCC 1 cut(s) 524
MlyI GAGTC 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 306
MreI CGCCGGCG 1 cut(s) 526
MroNI GCCGGC 1 cut(s) 526
MseI TTAA 1 cut(s) 668
MslI CAYNNNNRTG 1 cut(s) 590
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 6 cut(s) 26, 50, 134, 447, 527, 738
MspR9I CCNGG 6 cut(s) 26, 27, 50, 51, 409, 448
MunI CAATTG 1 cut(s) 466
MvaI CCWGG 1 cut(s) 409
MvnI CGCG 2 cut(s) 418, 420
MwoI GCNNNNNNNGC 6 cut(s) 51, 86, 113, 165, 171, 530
NaeI GCCGGC 1 cut(s) 528
NarI GGCGCC 1 cut(s) 524
NciI CCSGG 5 cut(s) 26, 27, 50, 51, 448
NdeII GATC 2 cut(s) 549, 701
NgoMIV GCCGGC 1 cut(s) 526
NlaIII CATG 5 cut(s) 184, 199, 251, 632, 649
NlaIV GGNNCC 5 cut(s) 32, 33, 148, 384, 525
NmeAIII GCCGAG 1 cut(s) 500
NmuCI GTSAC 1 cut(s) 568
NspI RCATGY 2 cut(s) 251, 632
PaeI GCATGC 1 cut(s) 251
PauI GCGCGC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdiI GCCGGC 1 cut(s) 528
PfeI GAWTC 1 cut(s) 266
PkrI GCNGC 6 cut(s) 39, 82, 103, 106, 167, 532
PleI GAGTC 1 cut(s) 357
PluTI GGCGCC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 357
Ppu21I YACGTR 1 cut(s) 657
PpuMI RGGWCCY 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 407
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 407
PspN4I GGNNCC 5 cut(s) 32, 33, 148, 384, 525
PspPI GGNCC 2 cut(s) 31, 147
PspPPI RGGWCCY 1 cut(s) 31
PteI GCGCGC 1 cut(s) 416
RsaI GTAC 2 cut(s) 395, 464
RsaNI GTAC 2 cut(s) 394, 463
RseI CAYNNNNRTG 1 cut(s) 590
SaqAI TTAA 1 cut(s) 668
SatI GCNGC 6 cut(s) 38, 81, 102, 105, 166, 531
Sau3AI GATC 2 cut(s) 549, 701
Sau96I GGNCC 2 cut(s) 31, 147
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 6 cut(s) 26, 27, 50, 51, 409, 448
SduI GDGCHC 1 cut(s) 387
SetI ASST 6 cut(s) 264, 381, 410, 575, 611, 659
SexAI ACCWGGT 1 cut(s) 407
SfaNI GCATC 2 cut(s) 177, 225
SfoI GGCGCC 1 cut(s) 525
SgrAI CRCCGGYG 1 cut(s) 526
SinI GGWCC 1 cut(s) 31
SmaI CCCGGG 2 cut(s) 27, 51
SmiMI CAYNNNNRTG 1 cut(s) 590
SphI GCATGC 1 cut(s) 251
Sse9I AATT 4 cut(s) 466, 500, 577, 724
SsiI CCGC 9 cut(s) 35, 38, 81, 102, 105, 163, 420, 530, 533
SspDI GGCGCC 1 cut(s) 523
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 6 cut(s) 24, 25, 48, 49, 407, 446
TaaI ACNGT 1 cut(s) 353
TaiI ACGT 3 cut(s) 381, 575, 659
TaqI TCGA 3 cut(s) 372, 504, 605
TaqII GACCGA 1 cut(s) 673
TasI AATT 4 cut(s) 466, 500, 577, 724
TatI WGTACW 1 cut(s) 462
TauI GCSGC 5 cut(s) 40, 83, 104, 107, 533
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 1 cut(s) 668
Tru9I TTAA 1 cut(s) 668
TscAI CASTG 1 cut(s) 161
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 1 cut(s) 165
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 3 cut(s) 17, 608, 662
TspGWI ACGGA 1 cut(s) 418
TspMI CCCGGG 2 cut(s) 25, 49
TspRI CASTG 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 500
XceI RCATGY 2 cut(s) 251, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmaI CCCGGG 2 cut(s) 25, 49
XspI CTAG 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.