Rmu_sc0000565.1_g000040

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000565.1
OG0021530
Physical Location & Seq
Forward (+)
152436 .. 161158
8723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 6108 bp
atgtattctcaatttggatcgccttcctccgcgcctctcacccggacacacgatgtgtttctgagttttagaggtgcggacactcgctacagtttcacaggccatttgtacagcgcactggctgagaaggggattcacaccttcgtggatgatgatgagcttagaagaggagaggaaatatcacaagaacttgtccaagcaattgaagggtcgaggatttctctcattgtattctcccaaaactatgcatcctcaaagtgttgtttggatgaactagttcatatccttgaatgtagaagatcaaagaaccaaatggtctggccaatcttttacaaagtggatccctcagatgtaaggcaccaaagaggtagttttggtatggcacttgcagagcatgaaagcagattcagaggtggcatggacaaggtgttaagatggagagcagctctttcagaagcagcaaatttgtctggatggcatttctcggacgggcatgaatctaaatttcttgttaatctcattgaagagatttctgcacaactaataaatcagacccatttgaatgtggcaaactttcctgttgggatagagtctcgtgtacaagatatgattcaacttctagatgttgaggacaatgatgttcggattgttgggatgtgcggaattggtggaatagggaagacaacaattgcaaaagctgtgtataattcgattggccatatgtttgatggaaggtgctttctagaaaatgttagagagaattccatggcacatgacggcctagtcaaactagaaagggttattctgtctgagattatggggcagaaaatattgaaggctaccaaagtggataaagagaaaaggttcaggtataagagaattcttttagttcttgatgatgtgaatcaattggaacagttagacacgttagctggactgtctgatttgtttggatggggcagcagactaataataacaacaaggaatcagcatttgctggctgcttatgaagtcagtgtaatatacaatgtcaaggaactaaatcatcatgaagcttctgagctcttctgtttaaatgcgtttaagaaaaatagaaatttcgatgatgtgaagttcccaattgatgatgcagtacagtatgctcaaggcctgccattagctttgattgttttaggttcacatctcaatggtaaaagtatcaagcaatggcaagctgcagttgatagttgcagaaatgttcgtactagagagattcaagaacttctcaagataagtttcaacgcattggagggtttggtgaaagaactttttcttgacattgcgtgtttttttaagggtgtaaataagaaccatgtgacacatatccttgaaggttgtggcctgaaccctaaatattgtattgaagtactcataaagaaggccttcatatattttaatgaagagaagctttggatgcatgacttgctagaagaaatggctaaagaaattgttgttcaggggtcacccttacaagctttcccctccaattttaatccaactgaacttgttaaacttgatatggctcgcagctgcatgtctggattcaagagtttgcaaaatctgaaatctctaagtctgaaaggctgcaatttcctgacagtaacacctgacttctctggattcccagccttagaggccttgaatctaaacttttgttcaagtttagttgaggttcatccttcagttggattccttgacaagcttgttagcctgagtcttaaaggatgtcacaacctcatattgttcccaagactagtcaagttgaaatctctgcgagatatcattcttagtggttgcaataggctagagaacttccctgaagttgtgggaaagatggactccttgacattgatgaatctatctgctactgccattgaacggctgcctgacttctctggattcccaaccttagaggacttgaatctaaacttttgttcaagtttagttgaggttcatccttcagttggattccttgacaagcttgttagcctgagtcttaaaggatgtcacaacctcatattgttcccaagactagtcaagttgaaatctctgcgagatatcaatcttagtggttgcaaaagactagagaacttccctgaagttgtgggaaagatggactccttgacattgatgaatctatctgctactgccattgaacggctgcctgacttctctggattcccaaccttagaggacttgaatctaaacttttgttcaagtttagttgaggttcatccttcagttggattccttgacaagcttgttagcctgagtcttaaaggatgtcgcaacctcaaattgttcccaagactagtcaagttgaaatctctgcgagatatcaatcttagtggttgcaaaagactagagaacttctgtgaagttgtgggaaagatggactccttgacatggatgaatctctccggcactgccattgaacagctgcctgcaattggaaatgattttatgaatctaaaagaattaaatttagcaggttgtgaaaaacttatgactttgccatgcagcatttatgacttgcaacatctgaattacctttgtgttcaggagtgcaaaaagcttgctttatttccaaatattgtgaactctaaagtgctttcaactaagtcgaagatctcatttgacaactataggtctttatcagaagcagaaagagatggtcaagggaatgtagccgatccggggctaggcctatctagtggcagagggtacaatttattgcttcctgagctagtagacttcaatatgtcaagatgcagattatctgactgtgatttcctcgttagtcttgattgttcctccaccttattagaaattgacctttctggaaacaattttgttagtcttcctgaatgtatcaccaaatttgtcaacttgaaatggctgttcttgtgtggttgcaaaaggcttcgagaaattcaagaacttccaccatgtatcaaatggttagatttgaaagattgcgtatcattggaaagaatttcaaagttgtcaaacattctgaatgataaagaatcagaaatgatggagtggatggacttgtctaattgccggagattgtgtgacactctgtttcaaatggcaaagagaaactctgtttcactgagtgatcagatcagcagtctcttctctttctttctctattttcggaactcttcatttggcgtggtgcttccaggaaatgagattccaaagtggttcggctggcatagagatttcaacaggcatcaaccgagtgaacagtttcctattgaaattccctcaaatttcatatgggagaacacaggattggctatctctgttgtttttgatcatccacactggaatttaggtgattttcttttgtcattcgatatttttattaatgaagctagggtgatggggtctgaccgttggattcattcaactgaatcggctcatgtttggctgcaatatattccattcaatacatttgatatgggtccgtttgattttaagcgtccattaccgcctttaaggtgtcaagttagtatccgccaaaatggcagcagtttggtcaacttggtgagtttcaaaagctgtggggtccaccttgtaatgccaccaaatgaagatgtctgtatgacggcatccgctactgataacctcagagatctccccaagtggggggtccttgattggcctggaattgtacctctaccattggagcggattattggactcaatttgtctggaagtgtcgtgttaccactgagagtggcattcgagtgttggaaaaatctgacatctataagttttgaaagctgcctatacataaaaaaattcccggacacatctggattgccaaatttagagaatctgaatctaaatgactgtacaagtttagttgaattagatgattctgttggagaattagataatcttgttgacttgagtctttctggatgccatcatcttattacgtttccaagaagaatcaccttgaaatctgtgaaacgtattaactttaaaggctgccgaatgctggagagcttgcccgaaattgtgcatgagatggaatgcttaacatggttggatctatctggcactgccatcaaagaattgccttcatcaattagatatcttagaagccttgaagagttggttttaaaggaatgtgaaaagctgaccaatctgccaagcaacatttatgagttgcaacatctatggtgtcttgatctccagaactgctcaaaccttgctgaatttccggattggagttcagagtctcttcaaattaacccaaagatttcacatgacttgtggtatcttaacctccgtggttgcaaaaggcttgaagaaattcctgagctgccaccgaaagtggaatggataaatgtggctgattgcctacgattgagaagatttgcaaagttgtcaaacattctggtacataaagagtcagaaatgatcaaatgtatcacaatgttcaattgtcagagactgtgtgacagtctggctcttgaaatggctgaaaaccgaaatattcttctgaatgaggtctctctctgctttctctttctctattgtaagcagtctgaattcgacatcgtgtttcctggaagtgaagttccaaagtggttcagctgtaggaaagatttgatggaattaattgataaatgtgagttttcttttgaaatccctccaaatttcaatttggggaacaagggattcgccatctgtgctgcagctgctgttgttgaacatattaacccagaagaaaaggagactatgcagcaatgctctttcacagcaacaattgatatcaatgcagatagaattgtcacccgatcgttttattttgaagtaaaagacattgaattagctcatgtgtggctttgctatattccattcgtgaagtttgtgtctcatcagtcgccaccttttaagtgtggagttagtttggaacatactagccaaggttcaatgcgctgtaaaagctttggggtccatctggtaattctgcaagatgaagatgaagatgaagatgaagatgaagatgatgaagatgaagatgatgaagatgaagatgagaaatcagttgagggaagatcgtatttggagtgcagtcgggtatctctcttgttgctatgggctttttgttcaatttcctcatccttgaaaagcttgcagagagtgtgctgtgtagttgacatatgccttaaatcaagtgcttcctttggagagtactatgcgggggcttccccgtctatgctgaacttattcaggaggaaactatcaccaaacccactatgccctatttgtgaagcccaggaagagtctttcgaacatatgctattcttgtgcccatgggtagaagcaatatggtttggatcccctttgggcatcagactcgataaacaacagttaactacaatagataaatggctattggaaagcctaaggagtatcccaaatgcagtacaaaagaagaagtttctcacctgggtcagcttcttatgttgggcgatctggaaagctcgatgtgcttttgtttaccagcagaaacctgtgtccccaaacttcgcccttcagtcaggtttgtctctagcctctgagttcttggaggccggtgtggtcgaaacagtggaacttgaatctccgagagataacttttccccaaagtggacaactccccctttacactttgccaaaataaactgtgacgcgtcttggaaggccccttctgccgcgggtcttggagtggtcgtcagagatcatacatgtttcttcttgggaggctcatctacccctgcaatttgcagctcgacaattgtggcagaaatcagaggcgttgttagccggtgtgaacctggctcctttgaagactgcgattgggactgggttcccagagaagcgaatagagctgctcatcatgcagcctggattgccaattggacgatcggcgtcgatggcagtcttgcaggactcgccgtacatggagttcgggacgatggcgggagcagtggatctgtcattcagctcctccgcaaatatcgagatcttcaagctcgagttatagtcagacgatcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

2035

Amino Acids

230.06

Weight (kDa)

5.67

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 183 5.6e-53 TIR domain
TIR_2 PF13676 19 - 115 3.3e-14 TIR domain
NB-ARC PF00931 198 - 364 2.6e-17 NB-ARC domain
WHD_ROQ1 PF23282 434 - 499 2.5e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 769 - 876 8e-08 Leucine-rich repeat region
LRR_13 PF23286 928 - 1019 2.3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 1340 - 1426 3.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 1355 - 1422 5.6e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021530)

Species Orthologous Gene IDs
rosa_laevigata RLG00000014343
rosa_multiflora Rmu_sc0000565.1_g000040
rosa_roxburghii Rroxscaffold_3G00228710 Rroxscaffold_7G00204260 Rroxscaffold_7G00204440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 782
Acc36I ACCTGC 1 cut(s) 2519
AccB1I GGYRCC 1 cut(s) 357
AccBSI CCGCTC 1 cut(s) 3742
AccI GTMKAC 1 cut(s) 2790
AccII CGCG 3 cut(s) 32, 5741, 5765
AccIII TCCGGA 1 cut(s) 4282
AclWI GGATC 8 cut(s) 25, 335, 348, 2726, 4116, 5400, 5413, 6049
AcoI YGGCCR 2 cut(s) 320, 715
AcuI CTGAAG 6 cut(s) 1704, 1878, 1983, 2157, 2262, 5588
AcyI GRCGYC 1 cut(s) 5979
AdeI CACNNNGTG 2 cut(s) 55, 3161
AflIII ACRYGT 3 cut(s) 924, 5739, 5795
AhlI ACTAGT 4 cut(s) 274, 1792, 2071, 2350
AjnI CCWGG 7 cut(s) 3229, 3715, 4630, 5343, 5516, 5884, 5954
AjuI GAANNNNNNNTTGG 2 cut(s) 3326, 3358
AleI CACNNNNGTG 1 cut(s) 143
AloI GAACNNNNNNTCC 2 cut(s) 4626, 4658
Alw21I GWGCWC 1 cut(s) 1065
Alw26I GTCTC 8 cut(s) 597, 3182, 4305, 4507, 4579, 4793, 4944, 5622
AlwI GGATC 8 cut(s) 25, 335, 348, 2726, 4116, 5400, 5413, 6049
AlwNI CAGNNNCTG 2 cut(s) 4516, 4766
Ama87I CYCGRG 1 cut(s) 6084
Aor13HI TCCGGA 1 cut(s) 4282
AseI ATTAAT 2 cut(s) 3417, 4682
Asp700I GAANNNNTTC 7 cut(s) 276, 863, 1308, 1421, 3297, 4374, 5294
AspLEI GCGC 3 cut(s) 34, 116, 5004
AspS9I GGNCC 5 cut(s) 3515, 3619, 3703, 5020, 5752
AsuC2I CCSGG 3 cut(s) 43, 2736, 3860
AsuII TTCGAA 1 cut(s) 5358
AvaI CYCGRG 1 cut(s) 6084
AvaII GGWCC 4 cut(s) 3515, 3619, 3703, 5020
AxyI CCTNAGG 1 cut(s) 5474
BaeGI GKGCMC 1 cut(s) 5381
BalI TGGCCA 2 cut(s) 322, 717
BamHI GGATCC 2 cut(s) 340, 5405
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 1 cut(s) 1065
BauI CACGAG 1 cut(s) 594
BbsI GAAGAC 3 cut(s) 686, 2891, 5904
Bbv12I GWGCWC 1 cut(s) 1065
BceAI ACGGC 5 cut(s) 793, 1934, 2213, 3675, 5990
BcgI CGANNNNNNTGC 2 cut(s) 5407, 5441
BciT130I CCWGG 7 cut(s) 3231, 3717, 4632, 5345, 5518, 5886, 5956
BciVI GTATCC 2 cut(s) 3575, 5492
BclI TGATCA 3 cut(s) 3163, 3364, 4482
BcnI CCSGG 3 cut(s) 43, 2736, 3860
BcoDI GTCTC 8 cut(s) 597, 3182, 4305, 4507, 4579, 4793, 4944, 5622
BcuI ACTAGT 4 cut(s) 274, 1792, 2071, 2350
BfmI CTRYAG 5 cut(s) 88, 1215, 2683, 4660, 4758
BfuAI ACCTGC 1 cut(s) 2519
BfuI GTATCC 2 cut(s) 3575, 5492
BglI GCCNNNNNGGC 2 cut(s) 1673, 3576
BglII AGATCT 3 cut(s) 2667, 3685, 6073
BmcAI AGTACT 2 cut(s) 1407, 5261
Bme18I GGWCC 4 cut(s) 3515, 3619, 3703, 5020
BmeT110I CYCGRG 1 cut(s) 6084
BmgT120I GGNCC 5 cut(s) 3515, 3619, 3703, 5020, 5752
BmrI ACTGGG 1 cut(s) 5923
BmsI GCATC 8 cut(s) 257, 1117, 1443, 2798, 3289, 3671, 3968, 5427
BmuI ACTGGG 1 cut(s) 5923
BoxI GACNNNNGTC 1 cut(s) 3966
BpiI GAAGAC 3 cut(s) 686, 2891, 5904
BplI GAGNNNNNCTC 4 cut(s) 205, 237, 430, 462
BpmI CTGGAG 2 cut(s) 4079, 4238
Bpu10I CCTNAGC 2 cut(s) 2781, 4380
Bpu14I TTCGAA 1 cut(s) 5358
BpuEI CTTGAG 3 cut(s) 1128, 1250, 3985
BpuMI CCSGG 3 cut(s) 43, 2736, 3860
BsaBI GATNNNNATC 3 cut(s) 903, 2100, 2379
BsaHI GRCGYC 1 cut(s) 5979
BsaI GGTCTC 1 cut(s) 4579
BsaJI CCNNGG 8 cut(s) 765, 2735, 4351, 4990, 5343, 5381, 5517, 5763
BsaWI WCCGGW 1 cut(s) 4282
BsaXI ACNNNNNCTCC 4 cut(s) 3100, 3130, 5766, 5796
Bse118I RCCGGY 2 cut(s) 5642, 5874
Bse1I ACTGG 3 cut(s) 123, 3380, 5918
Bse21I CCTNAGG 1 cut(s) 5474
Bse3DI GCAATG 3 cut(s) 1211, 1317, 4817
Bse8I GATNNNNATC 3 cut(s) 903, 2100, 2379
BseAI TCCGGA 1 cut(s) 4282
BseBI CCWGG 7 cut(s) 3231, 3717, 4632, 5345, 5518, 5886, 5956
BseDI CCNNGG 8 cut(s) 765, 2735, 4351, 4990, 5343, 5381, 5517, 5763
BseJI GATNNNNATC 3 cut(s) 903, 2100, 2379
BseMI GCAATG 3 cut(s) 1211, 1317, 4817
BseNI ACTGG 3 cut(s) 123, 3380, 5918
BseRI GAGGAG 2 cut(s) 183, 6047
BseSI GKGCMC 1 cut(s) 5381
BseYI CCCAGC 1 cut(s) 1663
BsgI GTGCAG 2 cut(s) 519, 5158
Bsh1236I CGCG 3 cut(s) 32, 5741, 5765
Bsh1285I CGRYCG 3 cut(s) 4865, 5976, 6104
BshNI GGYRCC 1 cut(s) 357
BsiEI CGRYCG 3 cut(s) 4865, 5976, 6104
BsiHKAI GWGCWC 1 cut(s) 1065
BsiHKCI CYCGRG 1 cut(s) 6084
BsiSI CCGG 8 cut(s) 43, 2460, 2735, 3106, 3860, 4283, 5643, 5875
BslFI GGGAC 3 cut(s) 5572, 5924, 6035
BsmAI GTCTC 8 cut(s) 597, 3182, 4305, 4507, 4579, 4793, 4944, 5622
BsmFI GGGAC 3 cut(s) 5572, 5924, 6035
BsmI GAATGC 3 cut(s) 3794, 4059, 4097
Bso31I GGTCTC 1 cut(s) 4579
BsoBI CYCGRG 1 cut(s) 6084
Bsp119I TTCGAA 1 cut(s) 5358
Bsp1286I GDGCHC 2 cut(s) 1065, 5381
Bsp13I TCCGGA 1 cut(s) 4282
Bsp1407I TGTACA 3 cut(s) 108, 598, 3908
Bsp19I CCATGG 2 cut(s) 765, 5381
BspEI TCCGGA 1 cut(s) 4282
BspFNI CGCG 3 cut(s) 32, 5741, 5765
BspHI TCATGA 1 cut(s) 1048
BspMAI CTGCAG 2 cut(s) 1219, 4762
BspMI ACCTGC 1 cut(s) 2519
BspPI GGATC 8 cut(s) 25, 335, 348, 2726, 4116, 5400, 5413, 6049
BspQI GCTCTTC 1 cut(s) 1070
BspT104I TTCGAA 1 cut(s) 5358
BspT107I GGYRCC 1 cut(s) 357
BspTNI GGTCTC 1 cut(s) 4579
BsrBI CCGCTC 1 cut(s) 3742
BsrDI GCAATG 3 cut(s) 1211, 1317, 4817
BsrFI RCCGGY 2 cut(s) 5642, 5874
BsrGI TGTACA 3 cut(s) 108, 598, 3908
BsrI ACTGG 3 cut(s) 123, 3380, 5918
BssAI RCCGGY 2 cut(s) 5642, 5874
BssECI CCNNGG 8 cut(s) 765, 2735, 4351, 4990, 5343, 5381, 5517, 5763
BssNI GRCGYC 1 cut(s) 5979
BssSI CACGAG 1 cut(s) 594
BssT1I CCWWGG 3 cut(s) 765, 4990, 5381
Bst2BI CACGAG 1 cut(s) 594
Bst2UI CCWGG 7 cut(s) 3231, 3717, 4632, 5345, 5518, 5886, 5956
Bst6I CTCTTC 9 cut(s) 160, 519, 1070, 1434, 3185, 3214, 4164, 4308, 5343
BstACI GRCGYC 1 cut(s) 5979
BstAPI GCANNNNNTGC 1 cut(s) 1462
BstAUI TGTACA 3 cut(s) 108, 598, 3908
BstBI TTCGAA 1 cut(s) 5358
BstC8I GCNNGC 9 cut(s) 999, 1151, 1212, 1564, 2484, 2616, 3260, 4067, 5201
BstDSI CCRYGG 4 cut(s) 765, 4351, 5381, 5763
BstEII GGTNACC 1 cut(s) 1500
BstFNI CGCG 3 cut(s) 32, 5741, 5765
BstHHI GCGC 3 cut(s) 34, 116, 5004
BstMAI GTCTC 8 cut(s) 597, 3182, 4305, 4507, 4579, 4793, 4944, 5622
BstMCI CGRYCG 3 cut(s) 4865, 5976, 6104
BstNI CCWGG 7 cut(s) 3231, 3717, 4632, 5345, 5518, 5886, 5956
BstNSI RCATGY 2 cut(s) 1576, 5799
BstPAI GACNNNNGTC 1 cut(s) 3966
BstPI GGTNACC 1 cut(s) 1500
BstSFI CTRYAG 5 cut(s) 88, 1215, 2683, 4660, 4758
BstSLI GKGCMC 1 cut(s) 5381
BstUI CGCG 3 cut(s) 32, 5741, 5765
BstV2I GAAGAC 3 cut(s) 686, 2891, 5904
BstX2I RGATCY 7 cut(s) 340, 2667, 3685, 4108, 5405, 6041, 6073
BstYI RGATCY 7 cut(s) 340, 2667, 3685, 4108, 5405, 6041, 6073
Bsu36I CCTNAGG 1 cut(s) 5474
BsuI GTATCC 2 cut(s) 3575, 5492
BtgI CCRYGG 4 cut(s) 765, 4351, 5381, 5763
BtsI GCAGTG 3 cut(s) 2463, 4119, 6043
BtsIMutI CAGTG 9 cut(s) 116, 1021, 2463, 3155, 3373, 3782, 4119, 5664, 6043
BveI ACCTGC 1 cut(s) 2519
Cac8I GCNNGC 9 cut(s) 999, 1151, 1212, 1564, 2484, 2616, 3260, 4067, 5201
CaiI CAGNNNCTG 2 cut(s) 4516, 4766
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 3 cut(s) 34, 116, 5004
Cfr10I RCCGGY 2 cut(s) 5642, 5874
Cfr13I GGNCC 5 cut(s) 3515, 3619, 3703, 5020, 5752
Cfr42I CCGCGG 1 cut(s) 5766
CseI GACGC 4 cut(s) 3521, 5730, 5747, 5968
CspCI CAANNNNNGTGG 6 cut(s) 2089, 2124, 2368, 2403, 3595, 3630
DraI TTTAAA 3 cut(s) 1074, 4042, 4182
DraIII CACNNNGTG 2 cut(s) 55, 3161
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 2 cut(s) 320, 715
Eam1104I CTCTTC 9 cut(s) 160, 519, 1070, 1434, 3185, 3214, 4164, 4308, 5343
EarI CTCTTC 9 cut(s) 160, 519, 1070, 1434, 3185, 3214, 4164, 4308, 5343
EciI GGCGGA 1 cut(s) 3557
Ecl136II GAGCTC 1 cut(s) 1063
Eco130I CCWWGG 3 cut(s) 765, 4990, 5381
Eco147I AGGCCT 4 cut(s) 1149, 1421, 1676, 2745
Eco24I GRGCYC 1 cut(s) 1065
Eco31I GGTCTC 1 cut(s) 4579
Eco32I GATATC 5 cut(s) 1819, 2098, 2377, 4154, 4837
Eco47I GGWCC 4 cut(s) 3515, 3619, 3703, 5020
Eco53kI GAGCTC 1 cut(s) 1063
Eco57I CTGAAG 6 cut(s) 1704, 1878, 1983, 2157, 2262, 5588
Eco81I CCTNAGG 1 cut(s) 5474
Eco88I CYCGRG 1 cut(s) 6084
Eco91I GGTNACC 1 cut(s) 1500
EcoICRI GAGCTC 1 cut(s) 1063
EcoO109I RGGNCCY 2 cut(s) 3703, 5752
EcoO65I GGTNACC 1 cut(s) 1500
EcoRI GAATTC 3 cut(s) 760, 879, 4613
EcoRII CCWGG 7 cut(s) 3229, 3715, 4630, 5343, 5516, 5884, 5954
EcoRV GATATC 5 cut(s) 1819, 2098, 2377, 4154, 4837
EcoT14I CCWWGG 3 cut(s) 765, 4990, 5381
EcoT22I ATGCAT 2 cut(s) 250, 1458
EcoT38I GRGCYC 1 cut(s) 1065
ErhI CCWWGG 3 cut(s) 765, 4990, 5381
FalI AAGNNNNNCTT 4 cut(s) 1406, 1438, 1431, 1463
FaqI GGGAC 3 cut(s) 5572, 5924, 6035
FauI CCCGC 3 cut(s) 5260, 5758, 6023
FauNDI CATATG 4 cut(s) 720, 3326, 5228, 5364
FbaI TGATCA 3 cut(s) 3163, 3364, 4482
FblI GTMKAC 1 cut(s) 2790
FriOI GRGCYC 1 cut(s) 1065
GlaI GCGC 3 cut(s) 33, 115, 5003
GsaI CCCAGC 1 cut(s) 1667
GsuI CTGGAG 2 cut(s) 4079, 4238
HapII CCGG 8 cut(s) 43, 2460, 2735, 3106, 3860, 4283, 5643, 5875
HgaI GACGC 4 cut(s) 3521, 5730, 5747, 5968
HhaI GCGC 3 cut(s) 34, 116, 5004
Hin1I GRCGYC 1 cut(s) 5979
Hin6I GCGC 3 cut(s) 32, 114, 5002
HinP1I GCGC 3 cut(s) 32, 114, 5002
HincII GTYRAC 5 cut(s) 2926, 3592, 3961, 5224, 5442
HindII GTYRAC 5 cut(s) 2926, 3592, 3961, 5224, 5442
HindIII AAGCTT 9 cut(s) 1053, 1445, 1512, 1739, 2018, 2297, 2612, 5011, 5197
HpaI GTTAAC 1 cut(s) 5442
HpaII CCGG 8 cut(s) 43, 2460, 2735, 3106, 3860, 4283, 5643, 5875
Hpy99I CGWCG 1 cut(s) 5984
HpyCH4IV ACGT 3 cut(s) 926, 3995, 4030
HpySE526I ACGT 3 cut(s) 926, 3995, 4030
Hsp92I GRCGYC 1 cut(s) 5979
HspAI GCGC 3 cut(s) 32, 114, 5002
Kpn2I TCCGGA 1 cut(s) 4282
Ksp22I TGATCA 3 cut(s) 3163, 3364, 4482
KspAI GTTAAC 1 cut(s) 5442
KspI CCGCGG 1 cut(s) 5766
LguI GCTCTTC 1 cut(s) 1070
LmnI GCTCC 4 cut(s) 3739, 5894, 6033, 6060
LweI GCATC 8 cut(s) 257, 1117, 1443, 2798, 3289, 3671, 3968, 5427
MaeII ACGT 3 cut(s) 926, 3995, 4030
MbiI CCGCTC 1 cut(s) 3742
MfeI CAATTG 9 cut(s) 201, 687, 908, 1119, 2487, 4504, 4830, 5844, 5965
MflI RGATCY 7 cut(s) 340, 2667, 3685, 4108, 5405, 6041, 6073
MhlI GDGCHC 2 cut(s) 1065, 5381
MlsI TGGCCA 2 cut(s) 322, 717
MluI ACGCGT 1 cut(s) 5739
MluNI TGGCCA 2 cut(s) 322, 717
MmeI TCCRAC 8 cut(s) 1559, 1705, 1984, 2263, 3428, 3785, 3919, 4086
Mox20I TGGCCA 2 cut(s) 322, 717
Mph1103I ATGCAT 2 cut(s) 250, 1458
MroI TCCGGA 1 cut(s) 4282
MroXI GAANNNNTTC 7 cut(s) 276, 863, 1308, 1421, 3297, 4374, 5294
MscI TGGCCA 2 cut(s) 322, 717
MslI CAYNNNNRTG 4 cut(s) 143, 561, 1185, 3799
Msp20I TGGCCA 2 cut(s) 322, 717
MspA1I CMGCKG 5 cut(s) 1569, 2479, 4659, 4763, 5765
MspI CCGG 8 cut(s) 43, 2460, 2735, 3106, 3860, 4283, 5643, 5875
MunI CAATTG 9 cut(s) 201, 687, 908, 1119, 2487, 4504, 4830, 5844, 5965
Mva1269I GAATGC 3 cut(s) 3794, 4059, 4097
MvaI CCWGG 7 cut(s) 3231, 3717, 4632, 5345, 5518, 5886, 5956
MvnI CGCG 3 cut(s) 32, 5741, 5765
NciI CCSGG 3 cut(s) 43, 2736, 3860
NcoI CCATGG 2 cut(s) 765, 5381
NdeI CATATG 4 cut(s) 720, 3326, 5228, 5364
NmuCI GTSAC 8 cut(s) 1354, 1500, 1766, 2045, 3116, 4520, 4855, 5735
NsiI ATGCAT 2 cut(s) 250, 1458
NspI RCATGY 2 cut(s) 1576, 5799
NspV TTCGAA 1 cut(s) 5358
OliI CACNNNNGTG 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 6084
PagI TCATGA 1 cut(s) 1048
PceI AGGCCT 4 cut(s) 1149, 1421, 1676, 2745
PciI ACATGT 1 cut(s) 5795
PciSI GCTCTTC 1 cut(s) 1070
PctI GAATGC 3 cut(s) 3794, 4059, 4097
PdmI GAANNNNTTC 7 cut(s) 276, 863, 1308, 1421, 3297, 4374, 5294
PfoI TCCNGGA 3 cut(s) 3229, 3858, 4630
Ple19I CGATCG 3 cut(s) 4865, 5976, 6104
PpuMI RGGWCCY 1 cut(s) 3703
PscI ACATGT 1 cut(s) 5795
PshAI GACNNNNGTC 1 cut(s) 3966
PshBI ATTAAT 2 cut(s) 3417, 4682
Psp124BI GAGCTC 1 cut(s) 1065
Psp5II RGGWCCY 1 cut(s) 3703
Psp6I CCWGG 7 cut(s) 3229, 3715, 4630, 5343, 5516, 5884, 5954
PspEI GGTNACC 1 cut(s) 1500
PspFI CCCAGC 1 cut(s) 1663
PspGI CCWGG 7 cut(s) 3229, 3715, 4630, 5343, 5516, 5884, 5954
PspPI GGNCC 5 cut(s) 3515, 3619, 3703, 5020, 5752
PspPPI RGGWCCY 1 cut(s) 3703
PspXI VCTCGAGB 1 cut(s) 6084
PsrI GAACNNNNNNTAC 2 cut(s) 6000, 6032
PstI CTGCAG 2 cut(s) 1219, 4762
PstNI CAGNNNCTG 2 cut(s) 4516, 4766
PsuI RGATCY 7 cut(s) 340, 2667, 3685, 4108, 5405, 6041, 6073
PvuI CGATCG 3 cut(s) 4865, 5976, 6104
PvuII CAGCTG 4 cut(s) 1569, 2479, 4659, 4763
RseI CAYNNNNRTG 4 cut(s) 143, 561, 1185, 3799
SacI GAGCTC 1 cut(s) 1065
SacII CCGCGG 1 cut(s) 5766
SapI GCTCTTC 1 cut(s) 1070
Sau96I GGNCC 5 cut(s) 3515, 3619, 3703, 5020, 5752
ScaI AGTACT 2 cut(s) 1407, 5261
SduI GDGCHC 2 cut(s) 1065, 5381
SfaNI GCATC 8 cut(s) 257, 1117, 1443, 2798, 3289, 3671, 3968, 5427
SfcI CTRYAG 5 cut(s) 88, 1215, 2683, 4660, 4758
Sfr274I CTCGAG 1 cut(s) 6084
Sfr303I CCGCGG 1 cut(s) 5766
SfuI TTCGAA 1 cut(s) 5358
SgrBI CCGCGG 1 cut(s) 5766
SinI GGWCC 4 cut(s) 3515, 3619, 3703, 5020
SlaI CTCGAG 1 cut(s) 6084
SmiMI CAYNNNNRTG 4 cut(s) 143, 561, 1185, 3799
SmlI CTYRAG 4 cut(s) 1143, 1265, 3964, 6084
SmoI CTYRAG 4 cut(s) 1143, 1265, 3964, 6084
SpeI ACTAGT 4 cut(s) 274, 1792, 2071, 2350
SseBI AGGCCT 4 cut(s) 1149, 1421, 1676, 2745
SspI AATATT 4 cut(s) 831, 1394, 2632, 4558
SstI GAGCTC 1 cut(s) 1065
StuI AGGCCT 4 cut(s) 1149, 1421, 1676, 2745
StyI CCWWGG 3 cut(s) 765, 4990, 5381
TaiI ACGT 3 cut(s) 929, 3998, 4033
TatI WGTACW 7 cut(s) 108, 598, 1132, 1405, 3908, 5259, 5494
TauI GCSGC 1 cut(s) 5765
TscAI CASTG 9 cut(s) 123, 1021, 2470, 3162, 3380, 3789, 4126, 5664, 6043
TseFI GTSAC 8 cut(s) 1354, 1500, 1766, 2045, 3116, 4520, 4855, 5735
Tsp45I GTSAC 8 cut(s) 1354, 1500, 1766, 2045, 3116, 4520, 4855, 5735
TspGWI ACGGA 2 cut(s) 3507, 4340
TspRI CASTG 9 cut(s) 123, 1021, 2470, 3162, 3380, 3789, 4126, 5664, 6043
VpaK11BI GGWCC 4 cut(s) 3515, 3619, 3703, 5020
VspI ATTAAT 2 cut(s) 3417, 4682
XbaI TCTAGA 2 cut(s) 619, 742
XceI RCATGY 2 cut(s) 1576, 5799
XcmI CCANNNNNNNNNTGG 2 cut(s) 725, 2994
XhoI CTCGAG 1 cut(s) 6084
XmiI GTMKAC 1 cut(s) 2790
XmnI GAANNNNTTC 7 cut(s) 276, 863, 1308, 1421, 3297, 4374, 5294
ZrmI AGTACT 2 cut(s) 1407, 5261
Zsp2I ATGCAT 2 cut(s) 250, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.