Rmu_sc0000762.1_g000014

actin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000762.1
OG0020545
Physical Location & Seq
Forward (+)
66778 .. 67964
1187 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
atgattgtcttctcgaggaggtatgtggattctaattggtgtcttgaggggctgttaaagatcatggagtgtagaaacacattggggcaaatagtcttcccagtgttatttaatgttgatccttcagatgtcaggaaacagagtggtacttttgcagaggcactcgatcggaaacataaagatatagatgaagatatacggtgcaaagttggagagcaactcttacagaagctgcagatttgtctggctgggatcttcaaaacacttccaatggattccgtcaagttagaccgttatgttgctgatgtggcactccaagccagaccacctcagcatttgcaggtcatgcaacctcccctcgccgcctcgtctgaaactgaattaaccaacctctctccctcctctcttactcaaagaactcaaactcgaattccctctgttgctgcaccactaaaacttgaacaggctcaaagtctccgagagcagtttcagagccaccatctccaaaaccaccttctcactaccaccttctactccatttgtcactacacccaatccgaccacccttaccctctaccacaaccattcccagacccacttctccaagccactgcctctcaactcctcatttctaatcgcccagatccctaccttcaccaccaccaaaactaccacaaccgccaccgagctcaaccaacaattccattggaatcaacaaattccaagaactacacttgcataatcaatcaagccaacatatccaacttatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 map04011
Pfam Domains
Protein Families
Drf_FH3 Drf_GBD FH2

Protein Analysis

256

Amino Acids

29.21

Weight (kDa)

7.71

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 61 9.2e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020545)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0072481
rosa_multiflora Rmu_sc0000762.1_g000014
rosa_roxburghii Rroxscaffold_3G00257690 Rroxscaffold_5G00347300 Rroxscaffold_6G00398670 Rroxscaffold_6G00409190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 331
AciI CCGC 2 cut(s) 363, 679
AclWI GGATC 3 cut(s) 113, 260, 638
AcsI RAATTY 2 cut(s) 429, 718
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 1 cut(s) 148
AgsI TTSAA 2 cut(s) 259, 461
AjuI GAANNNNNNNTTGG 2 cut(s) 498, 530
AluBI AGCT 2 cut(s) 232, 689
AluI AGCT 2 cut(s) 232, 689
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 3 cut(s) 113, 260, 638
AlwNI CAGNNNCTG 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 13
ApeKI GCWGC 2 cut(s) 232, 443
ApoI RAATTY 2 cut(s) 429, 718
AsuHPI GGTGA 1 cut(s) 647
AvaI CYCGRG 1 cut(s) 13
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 691
BbvCI CCTCAGC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 219, 430
BccI CCATC 1 cut(s) 507
BcoDI GTCTC 1 cut(s) 479
BfmI CTRYAG 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 3 cut(s) 233, 363, 444
BlsI GCNGC 3 cut(s) 234, 364, 445
BmeT110I CYCGRG 1 cut(s) 13
BmrI ACTGGG 1 cut(s) 95
BmuI ACTGGG 1 cut(s) 95
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 204, 236
Bpu10I CCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 3 cut(s) 31, 391, 614
BseXI GCAGC 2 cut(s) 219, 430
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 429
Bsh1285I CGRYCG 1 cut(s) 169
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 13
BsmAI GTCTC 1 cut(s) 479
BsoBI CYCGRG 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 5 cut(s) 60, 118, 166, 252, 643
BspACI CCGC 2 cut(s) 363, 679
BspCNI CTCAG 1 cut(s) 343
BspMAI CTGCAG 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 3 cut(s) 113, 260, 638
BsrI ACTGG 1 cut(s) 101
BssMI GATC 5 cut(s) 60, 118, 166, 252, 643
Bst4CI ACNGT 2 cut(s) 201, 293
BstAPI GCANNNNNTGC 1 cut(s) 346
BstDEI CTNAG 1 cut(s) 330
BstKTI GATC 5 cut(s) 63, 121, 169, 255, 646
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 5 cut(s) 60, 118, 166, 252, 643
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 308, 317, 346
BstSFI CTRYAG 1 cut(s) 233
BstV1I GCAGC 2 cut(s) 219, 430
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 252, 643
BstYI RGATCY 2 cut(s) 252, 643
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 108, 609
BveI ACCTGC 1 cut(s) 331
CaiI CAGNNNCTG 1 cut(s) 232
Csp6I GTAC 1 cut(s) 147
CviAII CATG 2 cut(s) 64, 346
CviJI RGCY 9 cut(s) 52, 232, 248, 320, 467, 495, 608, 689, 752
CviKI_1 RGCY 9 cut(s) 52, 232, 248, 320, 467, 495, 608, 689, 752
CviQI GTAC 1 cut(s) 147
DdeI CTNAG 1 cut(s) 330
DpnI GATC 5 cut(s) 62, 120, 168, 254, 645
DpnII GATC 5 cut(s) 60, 118, 166, 252, 643
Ecl136II GAGCTC 1 cut(s) 689
Eco24I GRGCYC 1 cut(s) 691
Eco53kI GAGCTC 1 cut(s) 689
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 689
EcoRI GAATTC 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 691
FaeI CATG 2 cut(s) 67, 349
FatI CATG 2 cut(s) 63, 345
Fnu4HI GCNGC 3 cut(s) 233, 363, 444
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 3 cut(s) 233, 363, 444
GluI GCNGC 3 cut(s) 233, 363, 444
GsaI CCCAGC 1 cut(s) 252
Hin1II CATG 2 cut(s) 67, 349
HinfI GANTC 3 cut(s) 29, 275, 710
HphI GGTGA 1 cut(s) 647
Hpy188I TCNGA 6 cut(s) 127, 171, 373, 479, 492, 559
Hpy188III TCNNGA 3 cut(s) 13, 44, 133
HpyAV CCTTC 4 cut(s) 132, 524, 538, 662
HpyCH4III ACNGT 2 cut(s) 201, 293
HpyCH4V TGCA 7 cut(s) 155, 204, 235, 340, 349, 446, 738
HpyF10VI GCNNNNNNNGC 3 cut(s) 308, 317, 346
HpyF3I CTNAG 1 cut(s) 330
Hsp92II CATG 2 cut(s) 67, 349
Kzo9I GATC 5 cut(s) 60, 118, 166, 252, 643
LpnPI CCDG 9 cut(s) 114, 118, 230, 234, 326, 334, 449, 603, 654
Lsp1109I GCAGC 2 cut(s) 219, 430
MaeIII GTNAC 1 cut(s) 542
MalI GATC 5 cut(s) 62, 120, 168, 254, 645
MboI GATC 5 cut(s) 60, 118, 166, 252, 643
MboII GAAGA 3 cut(s) 88, 203, 247
MflI RGATCY 2 cut(s) 252, 643
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 5 cut(s) 34, 380, 429, 699, 718
MmeI TCCRAC 2 cut(s) 190, 582
MseI TTAA 3 cut(s) 56, 111, 383
MwoI GCNNNNNNNGC 3 cut(s) 308, 317, 346
NdeII GATC 5 cut(s) 60, 118, 166, 252, 643
NlaIII CATG 2 cut(s) 67, 349
NmuCI GTSAC 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 13
PfeI GAWTC 3 cut(s) 29, 275, 710
PkrI GCNGC 3 cut(s) 234, 364, 445
Ple19I CGATCG 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 691
PspFI CCCAGC 1 cut(s) 248
PstI CTGCAG 1 cut(s) 237
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 252, 643
PvuI CGATCG 1 cut(s) 169
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SacI GAGCTC 1 cut(s) 691
SaqAI TTAA 3 cut(s) 56, 111, 383
SatI GCNGC 3 cut(s) 233, 363, 444
Sau3AI GATC 5 cut(s) 60, 118, 166, 252, 643
SduI GDGCHC 1 cut(s) 691
SfcI CTRYAG 1 cut(s) 233
Sfr274I CTCGAG 1 cut(s) 13
SlaI CTCGAG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 13, 44
SmoI CTYRAG 2 cut(s) 13, 44
Sse9I AATT 5 cut(s) 34, 380, 429, 699, 718
SsiI CCGC 2 cut(s) 363, 679
SstI GAGCTC 1 cut(s) 691
TaaI ACNGT 2 cut(s) 201, 293
TaqI TCGA 3 cut(s) 14, 165, 427
TasI AATT 5 cut(s) 34, 380, 429, 699, 718
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 3 cut(s) 29, 275, 710
Tru1I TTAA 3 cut(s) 56, 111, 383
Tru9I TTAA 3 cut(s) 56, 111, 383
TscAI CASTG 2 cut(s) 108, 616
TseFI GTSAC 1 cut(s) 542
TseI GCWGC 2 cut(s) 232, 443
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 1 cut(s) 204
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 2 cut(s) 108, 616
XapI RAATTY 2 cut(s) 429, 718
XhoI CTCGAG 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.