Rmu_sc0000768.1_g000025

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000768.1
OG0016900
Physical Location & Seq
Reverse (-)
73073 .. 76030
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
atgtcggcccaaaaagaaagaaagaaagcaaagatgctgatgatttctgaaatgaaaagagagaaggagacatatgagaaagcaaggatgctgaaggagtacatagaaaatatcagggctgcatacaccaaggattttacaagtgaagatggtgaaaagaggcagatggcagttgctacctatcttgtcgataaactttgtctgagggctggaaatgatacggatgatgatgaaattgatattgtcgagtgctgcaaattaaaagtagagaacgtcaaaacaattccccctaacacgttggagttcaacttcctcgttaaagattcaataagatgtgaaaatactgttgacgttgagcttcctgtttacaaggcaatcgaagagttcctggctggaaagaatggtagtgaaggtctatttgacaagttggagaataccagtaaattgaatgcgcatctagagggactgatgcctggccttacaggaaaagtattccgtacatacaatgcatgcattacattggatgaaatattgaacaaagaaactccctctggagctgtttcagagaaagcaggtgtttatagccaggcaaccagggaggttgctatcatctgtaattttcagcgtactgtcgcaaggactggtactgtacggatgtactggttaaaagagtacatgagtgagttgcagggcacattgacggagctgaaaacagatttggacagggcggtaatgggattacccccactgagaaattctaatcgaaaaccaaatagaaacctgacagatatggacagggcagtaatgggattacccccactgagaaattctaatggaaaacgaaagagaatcctgaccccagaagagttagagaggacgatagctgaaacaattcagatgattgaaaaactggaacaggatatgagaacccaagagaaccagaaatacacgtcagttggaatgcccaaaatcaagtatcttgaccctaggatcgctgttgcatggtgtaagcgaaatgaagttcctattaacatgatatacaccgagtattatatgccaatgtttgagtgggcaatggatgtggatatcgacttcagattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

367

Amino Acids

42.38

Weight (kDa)

6.21

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I PF01028 19 - 239 3.4e-68 Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc PF14370 300 - 367 1.2e-20 C-terminal topoisomerase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 563
Acc16I TGCGCA 1 cut(s) 453
Acc36I ACCTGC 1 cut(s) 563
AciI CCGC 1 cut(s) 728
AclWI GGATC 1 cut(s) 998
AcsI RAATTY 2 cut(s) 754, 826
AcuI CTGAAG 2 cut(s) 113, 1078
AfaI GTAC 7 cut(s) 101, 499, 628, 646, 651, 659, 674
AflIII ACRYGT 2 cut(s) 294, 948
AgsI TTSAA 5 cut(s) 307, 327, 448, 535, 905
AjiI CACGTC 1 cut(s) 951
AjnI CCWGG 4 cut(s) 387, 472, 585, 593
AjuI GAANNNNNNNTTGG 2 cut(s) 701, 733
AluBI AGCT 4 cut(s) 358, 557, 706, 884
AluI AGCT 4 cut(s) 358, 557, 706, 884
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 1 cut(s) 998
AlwNI CAGNNNCTG 1 cut(s) 1101
AoxI GGCC 2 cut(s) 6, 475
ApeKI GCWGC 2 cut(s) 119, 252
ApoI RAATTY 2 cut(s) 754, 826
Asp700I GAANNNNTTC 1 cut(s) 891
AspA2I CCTAGG 1 cut(s) 986
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 164
AvrII CCTAGG 1 cut(s) 986
BaeGI GKGCMC 1 cut(s) 695
BbvI GCAGC 2 cut(s) 106, 239
BccI CCATC 2 cut(s) 143, 160
BciT130I CCWGG 4 cut(s) 389, 474, 587, 595
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 2 cut(s) 458, 987
BfuAI ACCTGC 1 cut(s) 563
BisI GCNGC 2 cut(s) 120, 253
BlnI CCTAGG 1 cut(s) 986
BlsI GCNGC 2 cut(s) 121, 254
Bme1390I CCNGG 4 cut(s) 389, 474, 587, 595
BmgBI CACGTC 1 cut(s) 951
BmgT120I GGNCC 1 cut(s) 7
BmrFI CCNGG 4 cut(s) 389, 474, 587, 595
BmsI GCATC 4 cut(s) 24, 78, 459, 463
BpmI CTGGAG 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 129, 594, 986
Bse1I ACTGG 4 cut(s) 438, 646, 665, 915
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 4 cut(s) 389, 474, 587, 595
BseDI CCNNGG 3 cut(s) 129, 594, 986
BseGI GGATG 5 cut(s) 93, 229, 529, 660, 1084
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 3 cut(s) 194, 740, 812
BseNI ACTGG 4 cut(s) 438, 646, 665, 915
BseSI GKGCMC 1 cut(s) 695
BseXI GCAGC 2 cut(s) 106, 239
BshFI GGCC 2 cut(s) 8, 477
BslFI GGGAC 1 cut(s) 477
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 477
BsmI GAATGC 2 cut(s) 454, 966
BsnI GGCC 2 cut(s) 8, 477
Bsp1286I GDGCHC 1 cut(s) 695
Bsp143I GATC 1 cut(s) 990
BspACI CCGC 1 cut(s) 728
BspANI GGCC 2 cut(s) 8, 477
BspCNI CTCAG 3 cut(s) 195, 741, 813
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 1 cut(s) 998
BsrDI GCAATG 1 cut(s) 1080
BsrI ACTGG 4 cut(s) 438, 646, 665, 915
BssECI CCNNGG 3 cut(s) 129, 594, 986
BssMI GATC 1 cut(s) 990
BssT1I CCWWGG 2 cut(s) 129, 986
Bst2UI CCWGG 4 cut(s) 389, 474, 587, 595
Bst4CI ACNGT 3 cut(s) 346, 631, 649
Bst6I CTCTTC 2 cut(s) 375, 858
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 3 cut(s) 203, 749, 821
BstF5I GGATG 5 cut(s) 93, 229, 529, 660, 1084
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 1 cut(s) 993
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 1 cut(s) 990
BstNI CCWGG 4 cut(s) 389, 474, 587, 595
BstNSI RCATGY 1 cut(s) 513
BstSCI CCNGG 4 cut(s) 387, 472, 585, 593
BstSLI GKGCMC 1 cut(s) 695
BstV1I GCAGC 2 cut(s) 106, 239
BsuRI GGCC 2 cut(s) 8, 477
BtrI CACGTC 1 cut(s) 951
BtsCI GGATG 5 cut(s) 93, 229, 529, 660, 1084
BtsIMutI CAGTG 2 cut(s) 746, 818
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 1 cut(s) 511
CaiI CAGNNNCTG 1 cut(s) 1101
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 7 cut(s) 100, 498, 627, 645, 650, 658, 673
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 4 cut(s) 510, 676, 1002, 1033
CviQI GTAC 7 cut(s) 100, 498, 627, 645, 650, 658, 673
DdeI CTNAG 3 cut(s) 203, 749, 821
DpnI GATC 1 cut(s) 992
DpnII GATC 1 cut(s) 990
Eam1104I CTCTTC 2 cut(s) 375, 858
EarI CTCTTC 2 cut(s) 375, 858
Eco130I CCWWGG 2 cut(s) 129, 986
Eco32I GATATC 1 cut(s) 1087
Eco57I CTGAAG 2 cut(s) 113, 1078
EcoRII CCWGG 4 cut(s) 387, 472, 585, 593
EcoRV GATATC 1 cut(s) 1087
EcoT14I CCWWGG 2 cut(s) 129, 986
EcoT22I ATGCAT 2 cut(s) 511, 515
ErhI CCWWGG 2 cut(s) 129, 986
FaeI CATG 4 cut(s) 513, 679, 1005, 1036
FaqI GGGAC 1 cut(s) 477
FatI CATG 4 cut(s) 509, 675, 1001, 1032
FauNDI CATATG 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 120, 253
FokI GGATG 5 cut(s) 100, 236, 536, 667, 1091
Fsp4HI GCNGC 2 cut(s) 120, 253
FspAI RTGCGCAY 1 cut(s) 453
FspBI CTAG 2 cut(s) 458, 987
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 1 cut(s) 453
GluI GCNGC 2 cut(s) 120, 253
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 2 cut(s) 8, 477
HhaI GCGC 1 cut(s) 454
Hin1II CATG 4 cut(s) 513, 679, 1005, 1036
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 3 cut(s) 323, 849, 1098
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 2 cut(s) 349, 367
Hpy188I TCNGA 6 cut(s) 49, 204, 565, 897, 1097, 1103
Hpy188III TCNNGA 4 cut(s) 458, 552, 853, 980
Hpy8I GTNNAC 2 cut(s) 349, 367
HpyAV CCTTC 3 cut(s) 58, 88, 404
HpyCH4III ACNGT 3 cut(s) 346, 631, 649
HpyCH4IV ACGT 4 cut(s) 273, 296, 351, 950
HpyCH4V TGCA 6 cut(s) 122, 255, 509, 513, 688, 1001
HpyF3I CTNAG 3 cut(s) 203, 749, 821
HpySE526I ACGT 4 cut(s) 273, 296, 351, 950
Hsp92II CATG 4 cut(s) 513, 679, 1005, 1036
HspAI GCGC 1 cut(s) 452
Kzo9I GATC 1 cut(s) 990
LmnI GCTCC 2 cut(s) 554, 703
Lsp1109I GCAGC 2 cut(s) 106, 239
LweI GCATC 4 cut(s) 24, 78, 459, 463
MaeI CTAG 2 cut(s) 458, 987
MaeII ACGT 4 cut(s) 273, 296, 351, 950
MalI GATC 1 cut(s) 992
MboI GATC 1 cut(s) 990
MboII GAAGA 3 cut(s) 158, 392, 875
MhlI GDGCHC 1 cut(s) 695
MluCI AATT 8 cut(s) 234, 257, 282, 443, 616, 754, 826, 891
MmeI TCCRAC 3 cut(s) 279, 408, 937
MnlI CCTC 7 cut(s) 153, 198, 323, 454, 559, 592, 867
Mph1103I ATGCAT 2 cut(s) 511, 515
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 4 cut(s) 260, 318, 665, 1029
MspR9I CCNGG 4 cut(s) 389, 474, 587, 595
Mva1269I GAATGC 2 cut(s) 454, 966
MvaI CCWGG 4 cut(s) 389, 474, 587, 595
NdeI CATATG 1 cut(s) 73
NdeII GATC 1 cut(s) 990
NlaIII CATG 4 cut(s) 513, 679, 1005, 1036
NsbI TGCGCA 1 cut(s) 453
NsiI ATGCAT 2 cut(s) 511, 515
NspI RCATGY 1 cut(s) 513
PaeI GCATGC 1 cut(s) 513
PaqCI CACCTGC 1 cut(s) 563
PctI GAATGC 2 cut(s) 454, 966
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 3 cut(s) 323, 849, 1098
PkrI GCNGC 2 cut(s) 121, 254
Psp6I CCWGG 4 cut(s) 387, 472, 585, 593
PspGI CCWGG 4 cut(s) 387, 472, 585, 593
PspPI GGNCC 1 cut(s) 7
PsrI GAACNNNNNNTAC 2 cut(s) 929, 961
PstNI CAGNNNCTG 1 cut(s) 1101
RsaI GTAC 7 cut(s) 101, 499, 628, 646, 651, 659, 674
RsaNI GTAC 7 cut(s) 100, 498, 627, 645, 650, 658, 673
SaqAI TTAA 4 cut(s) 260, 318, 665, 1029
SatI GCNGC 2 cut(s) 120, 253
Sau3AI GATC 1 cut(s) 990
Sau96I GGNCC 1 cut(s) 7
ScrFI CCNGG 4 cut(s) 389, 474, 587, 595
SduI GDGCHC 1 cut(s) 695
SfaNI GCATC 4 cut(s) 24, 78, 459, 463
SphI GCATGC 1 cut(s) 513
Sse9I AATT 8 cut(s) 234, 257, 282, 443, 616, 754, 826, 891
SsiI CCGC 1 cut(s) 728
SspI AATATT 1 cut(s) 531
SspMI CTAG 2 cut(s) 458, 987
StyD4I CCNGG 4 cut(s) 387, 472, 585, 593
StyI CCWWGG 2 cut(s) 129, 986
TaaI ACNGT 3 cut(s) 346, 631, 649
TaiI ACGT 4 cut(s) 276, 299, 354, 953
TaqI TCGA 5 cut(s) 189, 246, 378, 763, 1089
TasI AATT 8 cut(s) 234, 257, 282, 443, 616, 754, 826, 891
TatI WGTACW 3 cut(s) 99, 657, 672
TfiI GAWTC 3 cut(s) 323, 849, 1098
Tru1I TTAA 4 cut(s) 260, 318, 665, 1029
Tru9I TTAA 4 cut(s) 260, 318, 665, 1029
TscAI CASTG 2 cut(s) 753, 825
TseI GCWGC 2 cut(s) 119, 252
TspDTI ATGAA 4 cut(s) 68, 246, 540, 1032
TspGWI ACGGA 4 cut(s) 236, 485, 667, 716
TspRI CASTG 2 cut(s) 753, 825
XapI RAATTY 2 cut(s) 754, 826
XbaI TCTAGA 1 cut(s) 457
XceI RCATGY 1 cut(s) 513
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmaJI CCTAGG 1 cut(s) 986
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 2 cut(s) 458, 987
Zsp2I ATGCAT 2 cut(s) 511, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.