Rmu_sc0001169.1_g000013

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001169.1
OG0019305
Physical Location & Seq
Reverse (-)
88297 .. 91464
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
atgctgcaactgggtcaatcatcagtccttccacatccaattgataatgattctcatgacagaattgttctttgcatccgtacactatgcaataaagatgaagaagtgaagaagatatggttaaaatcttgtagagagagctttgttaaaatgcttgaagataagaagcttcgggaaatagaggaaagtagagctatggcgcagatatcaaatgcacaaccagatgaccttattgatttctaccatcttaagagcagaaagggtatgagccagcttgagttggaagatgaggttcaagatgatctcaaacgtgctactggagagtttgtaaaggatggagatgatgcaaataaactcaaccgcattcttcagctcacaggattcagcgatcctgtttatgctgaagcctatgttacagttaatcattatgacattgttctcgatgttaccgtcatcaaccaaaccaagggaacgcttcagaatttatgcttggagttggccacgatgggcgatcttaaacttgttgagcgtcctcagaactatacactagctccagaatcaaccaaacagataaaggcaaacattaaggtctcctcaactgaaacaggagttatatttggtaacattgtttatgagacgtcaaatgtgcttgagagaacagttattgttcttaatgacatccatattgatataatggattacatctctccttcttcatgtgctgatgtgttgtttcggacaatgtgggctgagtttgaatgggaaataaggtacgttagagttgctataagtactgttatcaaagacgagaaggactttctgaaccacatcgttaaagcaactaacatgaagtgcctgactccaccatcggcactggaaggagagtgtggagtcctcgctgccaatctgtatgcaaagagtgttttcggggaggatgctttggtgaatgtgagtgttgagaaacaagccgatggtaagctaagtggatacatcagaattagaagcaagactcaaggtattgcactgagtcttggggacaagatcaccttgaaacagaagggcggcccttga

Protein Analysis

356

Amino Acids

39.98

Weight (kDa)

5.27

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatamer_beta_C PF07718 74 - 212 5.4e-56 Coatomer beta C-terminal region
Coatomer_b_Cpla PF14806 218 - 348 2.4e-52 Coatomer beta subunit appendage platform
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019305)

Species Orthologous Gene IDs
prunus_persica Prupe.3G018100_v2.0.a1
rosa_multiflora Rmu_co8391663.1_g000001 Rmu_sc0001169.1_g000013 Rmu_sc0034979.1_g000001
rosa_samantha Rh2BG489000 Rh2BG489100 Rh2DG498000 Rh2DG498100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 641
AciI CCGC 2 cut(s) 361, 1062
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 3 cut(s) 353, 423, 461
AcyI GRCGYC 1 cut(s) 638
AfaI GTAC 3 cut(s) 82, 773, 793
AfiI CCNNNNNNNGG 1 cut(s) 466
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 4 cut(s) 158, 296, 758, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 7 cut(s) 141, 169, 194, 274, 373, 551, 979
AluI AGCT 7 cut(s) 141, 169, 194, 274, 373, 551, 979
Alw26I GTCTC 2 cut(s) 595, 629
AlwI GGATC 1 cut(s) 383
AoxI GGCC 2 cut(s) 498, 1063
ApeKI GCWGC 2 cut(s) 4, 899
ApoI RAATTY 1 cut(s) 481
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 1064
AsuHPI GGTGA 2 cut(s) 955, 1036
BalI TGGCCA 1 cut(s) 500
BbvI GCAGC 1 cut(s) 886
BccI CCATC 5 cut(s) 252, 329, 499, 874, 965
BciVI GTATCC 1 cut(s) 980
BcoDI GTCTC 2 cut(s) 595, 629
BfaI CTAG 1 cut(s) 548
BfrI CTTAAG 1 cut(s) 248
BfuI GTATCC 1 cut(s) 980
BisI GCNGC 3 cut(s) 5, 900, 1063
BlsI GCNGC 3 cut(s) 6, 901, 1064
BmcAI AGTACT 1 cut(s) 793
BmgT120I GGNCC 1 cut(s) 1064
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 84, 334, 925
BmuI ACTGGG 1 cut(s) 20
BpmI CTGGAG 2 cut(s) 339, 537
BpuEI CTTGAG 3 cut(s) 296, 671, 996
BsaHI GRCGYC 1 cut(s) 638
BsaI GGTCTC 1 cut(s) 595
BsaJI CCNNGG 1 cut(s) 465
BsaXI ACNNNNNCTCC 4 cut(s) 312, 342, 535, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 3 cut(s) 15, 322, 879
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 5 cut(s) 34, 75, 340, 678, 940
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 548, 741, 1016
BseNI ACTGG 3 cut(s) 15, 322, 879
BseRI GAGGAG 1 cut(s) 583
BseXI GCAGC 1 cut(s) 886
BshFI GGCC 2 cut(s) 500, 1065
BslFI GGGAC 1 cut(s) 1049
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 2 cut(s) 595, 629
BsmBI CGTCTC 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 1049
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 2 cut(s) 500, 1065
Bso31I GGTCTC 1 cut(s) 595
Bsp143I GATC 4 cut(s) 301, 388, 511, 1041
BspACI CCGC 2 cut(s) 361, 1062
BspANI GGCC 2 cut(s) 500, 1065
BspCNI CTCAG 3 cut(s) 547, 742, 1017
BspHI TCATGA 1 cut(s) 55
BspPI GGATC 1 cut(s) 383
BspTI CTTAAG 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 595
BsrI ACTGG 3 cut(s) 15, 322, 879
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 4 cut(s) 301, 388, 511, 1041
BssNI GRCGYC 1 cut(s) 638
BssT1I CCWWGG 1 cut(s) 465
Bst4CI ACNGT 4 cut(s) 418, 451, 661, 796
BstACI GRCGYC 1 cut(s) 638
BstAFI CTTAAG 1 cut(s) 248
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 4 cut(s) 534, 750, 980, 1025
BstF5I GGATG 5 cut(s) 34, 75, 340, 678, 940
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 4 cut(s) 304, 391, 514, 1044
BstMAI GTCTC 2 cut(s) 595, 629
BstMBI GATC 4 cut(s) 301, 388, 511, 1041
BstV1I GCAGC 1 cut(s) 886
BsuI GTATCC 1 cut(s) 980
BsuRI GGCC 2 cut(s) 500, 1065
BtsCI GGATG 5 cut(s) 34, 75, 340, 678, 940
BtsIMutI CAGTG 2 cut(s) 872, 1022
Cac8I GCNNGC 1 cut(s) 272
CciI TCATGA 1 cut(s) 55
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 1064
CseI GACGC 1 cut(s) 518
Csp6I GTAC 3 cut(s) 81, 772, 792
CviAII CATG 3 cut(s) 56, 717, 847
CviQI GTAC 3 cut(s) 81, 772, 792
DdeI CTNAG 4 cut(s) 534, 750, 980, 1025
DpnI GATC 4 cut(s) 303, 390, 513, 1043
DpnII GATC 4 cut(s) 301, 388, 511, 1041
EaeI YGGCCR 1 cut(s) 498
Eco130I CCWWGG 1 cut(s) 465
Eco31I GGTCTC 1 cut(s) 595
Eco32I GATATC 1 cut(s) 207
Eco57I CTGAAG 3 cut(s) 353, 423, 461
EcoRV GATATC 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 629
FaeI CATG 3 cut(s) 59, 720, 850
FalI AAGNNNNNCTT 2 cut(s) 1031, 1063
FaqI GGGAC 1 cut(s) 1049
FatI CATG 3 cut(s) 55, 716, 846
Fnu4HI GCNGC 3 cut(s) 5, 900, 1063
FokI GGATG 5 cut(s) 21, 62, 347, 665, 947
Fsp4HI GCNGC 3 cut(s) 5, 900, 1063
FspBI CTAG 1 cut(s) 548
GlaI GCGC 1 cut(s) 201
GluI GCNGC 3 cut(s) 5, 900, 1063
GsuI CTGGAG 2 cut(s) 339, 537
HaeIII GGCC 2 cut(s) 500, 1065
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 638
Hin1II CATG 3 cut(s) 59, 720, 850
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 7 cut(s) 50, 381, 557, 859, 891, 1009, 1027
HphI GGTGA 2 cut(s) 955, 1036
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 5 cut(s) 480, 537, 738, 822, 995
Hpy188III TCNNGA 5 cut(s) 56, 173, 296, 440, 554
Hpy8I GTNNAC 1 cut(s) 83
HpyAV CCTTC 5 cut(s) 38, 720, 805, 872, 1051
HpyCH4III ACNGT 4 cut(s) 418, 451, 661, 796
HpyCH4IV ACGT 3 cut(s) 310, 638, 774
HpyCH4V TGCA 7 cut(s) 7, 75, 90, 215, 347, 914, 1022
HpyF3I CTNAG 4 cut(s) 534, 750, 980, 1025
HpySE526I ACGT 3 cut(s) 310, 638, 774
Hsp92I GRCGYC 1 cut(s) 638
Hsp92II CATG 3 cut(s) 59, 720, 850
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 4 cut(s) 301, 388, 511, 1041
LmnI GCTCC 1 cut(s) 556
LpnPI CCDG 9 cut(s) 234, 284, 303, 363, 405, 567, 591, 860, 869
Lsp1109I GCAGC 1 cut(s) 886
LweI GCATC 3 cut(s) 84, 334, 925
MaeI CTAG 1 cut(s) 548
MaeII ACGT 3 cut(s) 310, 638, 774
MaeIII GTNAC 3 cut(s) 412, 445, 620
MalI GATC 4 cut(s) 303, 390, 513, 1043
MboI GATC 4 cut(s) 301, 388, 511, 1041
MboII GAAGA 7 cut(s) 113, 121, 124, 170, 296, 359, 705
MfeI CAATTG 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 4 cut(s) 39, 63, 481, 996
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 4 cut(s) 853, 900, 1003, 1036
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 6 cut(s) 175, 283, 543, 604, 905, 925
Mox20I TGGCCA 1 cut(s) 500
MscI TGGCCA 1 cut(s) 500
MseI TTAA 8 cut(s) 122, 147, 249, 420, 516, 585, 672, 834
Msp20I TGGCCA 1 cut(s) 500
MspCI CTTAAG 1 cut(s) 248
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 1 cut(s) 363
NdeII GATC 4 cut(s) 301, 388, 511, 1041
NlaIII CATG 3 cut(s) 59, 720, 850
PagI TCATGA 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 1 cut(s) 363
PfeI GAWTC 3 cut(s) 50, 381, 557
PkrI GCNGC 3 cut(s) 6, 901, 1064
PleI GAGTC 4 cut(s) 853, 899, 1003, 1035
PpsI GAGTC 4 cut(s) 853, 899, 1003, 1035
PspPI GGNCC 1 cut(s) 1064
RsaI GTAC 3 cut(s) 82, 773, 793
RsaNI GTAC 3 cut(s) 81, 772, 792
SaqAI TTAA 8 cut(s) 122, 147, 249, 420, 516, 585, 672, 834
SatI GCNGC 3 cut(s) 5, 900, 1063
Sau3AI GATC 4 cut(s) 301, 388, 511, 1041
Sau96I GGNCC 1 cut(s) 1064
ScaI AGTACT 1 cut(s) 793
SchI GAGTC 4 cut(s) 853, 900, 1003, 1036
SfaNI GCATC 3 cut(s) 84, 334, 925
SmlI CTYRAG 4 cut(s) 248, 275, 650, 1011
SmoI CTYRAG 4 cut(s) 248, 275, 650, 1011
Sse9I AATT 4 cut(s) 39, 63, 481, 996
SsiI CCGC 2 cut(s) 361, 1062
SspMI CTAG 1 cut(s) 548
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 4 cut(s) 418, 451, 661, 796
TaiI ACGT 3 cut(s) 313, 641, 777
TaqI TCGA 1 cut(s) 441
TasI AATT 4 cut(s) 39, 63, 481, 996
TatI WGTACW 1 cut(s) 791
TauI GCSGC 1 cut(s) 1065
TfiI GAWTC 3 cut(s) 50, 381, 557
Tru1I TTAA 8 cut(s) 122, 147, 249, 420, 516, 585, 672, 834
Tru9I TTAA 8 cut(s) 122, 147, 249, 420, 516, 585, 672, 834
TscAI CASTG 2 cut(s) 879, 1029
TseI GCWGC 2 cut(s) 4, 899
TspDTI ATGAA 3 cut(s) 114, 705, 863
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 2 cut(s) 879, 1029
Vha464I CTTAAG 1 cut(s) 248
XapI RAATTY 1 cut(s) 481
XspI CTAG 1 cut(s) 548
ZraI GACGTC 1 cut(s) 639
ZrmI AGTACT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.