Rmu_sc0001258.1_g000008

voltage-gated potassium channel subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001258.1
OG0018681
Physical Location & Seq
Forward (+)
35520 .. 36902
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
atggcgaaggcgaagtcgctgctccagtgctgcctcgaccacggcgtcaacttcttcgacaacgcggaggtctacgcgagcggccgcgccgaggagatcatgggccaggccatacgcgatctgggctggaagcgctccgacatcgtcgtctccaccaagatcagagatttagatcagaagactgagaattggggatcagacttcgttttgcggcttagggaccaaaagattcaaatcatcttgctcggcctattcccaccgccgatagctgatcaaacaccgctgttgcgtctcaatccaccttcatccaccgcagatgcttctggttcctcccacttcaaatcttacagcaaattgaaattgtgcattgaggcttcgccgatcactccaagttcatccccgccgtcgacgacttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

138

Amino Acids

15.16

Weight (kDa)

7.71

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 3 - 61 2.2e-11 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 44, 146
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 2 cut(s) 72, 407
AccII CGCG 4 cut(s) 65, 77, 87, 117
AciI CCGC 8 cut(s) 65, 81, 85, 211, 260, 281, 312, 401
AclWI GGATC 1 cut(s) 202
AcoI YGGCCR 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 45
AfeI AGCGCT 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 3 cut(s) 233, 340, 358
AjnI CCWGG 1 cut(s) 105
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
Alw26I GTCTC 2 cut(s) 154, 296
AlwI GGATC 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 134
AoxI GGCC 4 cut(s) 82, 103, 108, 247
ApeKI GCWGC 2 cut(s) 19, 30
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspLEI GCGC 2 cut(s) 89, 135
AspS9I GGNCC 2 cut(s) 103, 220
AvaII GGWCC 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 2 cut(s) 6, 17
BceAI ACGGC 2 cut(s) 58, 388
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 107
BclI TGATCA 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 154, 296
BfoI RGCGCY 1 cut(s) 136
BisI GCNGC 5 cut(s) 20, 31, 82, 85, 212
BlsI GCNGC 5 cut(s) 21, 32, 83, 86, 213
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 2 cut(s) 103, 220
BmiI GGNNCC 2 cut(s) 221, 328
BmrFI CCNGG 1 cut(s) 107
BmsI GCATC 1 cut(s) 307
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 215
BsaBI GATNNNNATC 2 cut(s) 171, 233
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 40, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 25
Bse8I GATNNNNATC 2 cut(s) 171, 233
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 2 cut(s) 40, 90
BseGI GGATG 2 cut(s) 305, 395
BseJI GATNNNNATC 2 cut(s) 171, 233
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 25
BseRI GAGGAG 1 cut(s) 107
BseX3I CGGCCG 1 cut(s) 82
BseXI GCAGC 2 cut(s) 6, 17
Bsh1236I CGCG 4 cut(s) 65, 77, 87, 117
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 4 cut(s) 84, 105, 110, 249
BsiEI CGRYCG 1 cut(s) 85
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 2 cut(s) 154, 296
BsmBI CGTCTC 2 cut(s) 154, 296
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 4 cut(s) 84, 105, 110, 249
Bsp143I GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
BspACI CCGC 8 cut(s) 65, 81, 85, 211, 260, 281, 312, 401
BspANI GGCC 4 cut(s) 84, 105, 110, 249
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 4 cut(s) 65, 77, 87, 117
BspLI GGNNCC 2 cut(s) 221, 328
BspPI GGATC 1 cut(s) 202
BsrBI CCGCTC 1 cut(s) 81
BsrI ACTGG 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 40, 90
BssMI GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 107
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 3 cut(s) 183, 215, 414
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 305, 395
BstFNI CGCG 4 cut(s) 65, 77, 87, 117
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 2 cut(s) 89, 135
BstKTI GATC 7 cut(s) 99, 121, 162, 175, 197, 274, 384
BstMAI GTCTC 2 cut(s) 154, 296
BstMBI GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 2 cut(s) 123, 132
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstUI CGCG 4 cut(s) 65, 77, 87, 117
BstV1I GCAGC 2 cut(s) 6, 17
BstV2I GAAGAC 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 82
BsuRI GGCC 4 cut(s) 84, 105, 110, 249
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 305, 395
BtsIMutI CAGTG 1 cut(s) 32
Cac8I GCNNGC 1 cut(s) 79
CciNI GCGGCCGC 1 cut(s) 82
CfoI GCGC 2 cut(s) 89, 135
Cfr13I GGNCC 2 cut(s) 103, 220
CseI GACGC 2 cut(s) 34, 278
CviAII CATG 1 cut(s) 100
CviJI RGCY 8 cut(s) 84, 105, 110, 126, 214, 249, 269, 374
CviKI_1 RGCY 8 cut(s) 84, 105, 110, 126, 214, 249, 269, 374
DdeI CTNAG 3 cut(s) 183, 215, 414
DpnI GATC 7 cut(s) 98, 120, 161, 174, 196, 273, 383
DpnII GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
DrdI GACNNNNNNGTC 2 cut(s) 44, 146
DseDI GACNNNNNNGTC 2 cut(s) 44, 146
EaeI YGGCCR 1 cut(s) 82
EagI CGGCCG 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 220
Eco47III AGCGCT 1 cut(s) 134
Eco52I CGGCCG 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 105
Esp3I CGTCTC 2 cut(s) 154, 296
FaeI CATG 1 cut(s) 103
FaiI YATR 2 cut(s) 101, 113
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 99
FauI CCCGC 1 cut(s) 408
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 2 cut(s) 72, 407
Fnu4HI GCNGC 5 cut(s) 20, 31, 82, 85, 212
FokI GGATG 2 cut(s) 292, 382
Fsp4HI GCNGC 5 cut(s) 20, 31, 82, 85, 212
GlaI GCGC 2 cut(s) 88, 134
GluI GCNGC 5 cut(s) 20, 31, 82, 85, 212
GsuI CTGGAG 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 4 cut(s) 84, 105, 110, 249
HgaI GACGC 2 cut(s) 34, 278
HhaI GCGC 2 cut(s) 89, 135
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 2 cut(s) 87, 133
HinP1I GCGC 2 cut(s) 87, 133
HincII GTYRAC 2 cut(s) 49, 408
HindII GTYRAC 2 cut(s) 49, 408
HinfI GANTC 1 cut(s) 229
Hpy166II GTNNAC 3 cut(s) 49, 73, 408
Hpy188I TCNGA 4 cut(s) 139, 164, 177, 199
Hpy8I GTNNAC 3 cut(s) 49, 73, 408
Hpy99I CGWCG 3 cut(s) 149, 409, 412
HpyAV CCTTC 1 cut(s) 312
HpyCH4V TGCA 1 cut(s) 366
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 132
HpyF3I CTNAG 3 cut(s) 183, 215, 414
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 2 cut(s) 87, 133
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
LmnI GCTCC 2 cut(s) 27, 140
LpnPI CCDG 6 cut(s) 38, 92, 107, 112, 119, 309
Lsp1109I GCAGC 2 cut(s) 6, 17
LweI GCATC 1 cut(s) 307
MalI GATC 7 cut(s) 98, 120, 161, 174, 196, 273, 383
MbiI CCGCTC 1 cut(s) 81
MboI GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
MboII GAAGA 2 cut(s) 46, 190
MluCI AATT 3 cut(s) 187, 353, 359
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 5 cut(s) 44, 61, 85, 340, 364
MspA1I CMGCKG 1 cut(s) 283
MspR9I CCNGG 1 cut(s) 107
MvaI CCWGG 1 cut(s) 107
MvnI CGCG 4 cut(s) 65, 77, 87, 117
MwoI GCNNNNNNNGC 2 cut(s) 123, 132
NdeII GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 2 cut(s) 221, 328
NmeAIII GCCGAG 2 cut(s) 115, 225
NotI GCGGCCGC 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 1 cut(s) 229
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 5 cut(s) 21, 32, 83, 86, 213
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 221, 328
PspPI GGNCC 2 cut(s) 103, 220
PsyI GACNNNGTC 1 cut(s) 143
SalI GTCGAC 1 cut(s) 406
SatI GCNGC 5 cut(s) 20, 31, 82, 85, 212
Sau3AI GATC 7 cut(s) 96, 118, 159, 172, 194, 271, 381
Sau96I GGNCC 2 cut(s) 103, 220
ScrFI CCNGG 1 cut(s) 107
SetI ASST 3 cut(s) 72, 271, 304
SfaNI GCATC 1 cut(s) 307
SgrDI CGTCGACG 1 cut(s) 406
SinI GGWCC 1 cut(s) 220
Sse9I AATT 3 cut(s) 187, 353, 359
SsiI CCGC 8 cut(s) 65, 81, 85, 211, 260, 281, 312, 401
StyD4I CCNGG 1 cut(s) 105
TaqI TCGA 3 cut(s) 36, 57, 407
TasI AATT 3 cut(s) 187, 353, 359
TauI GCSGC 3 cut(s) 84, 87, 214
TfiI GAWTC 1 cut(s) 229
TscAI CASTG 1 cut(s) 32
TseI GCWGC 2 cut(s) 19, 30
TspDTI ATGAA 2 cut(s) 294, 384
TspRI CASTG 1 cut(s) 32
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 72, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.