Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0001394.1

Orthogroup ID

OG0011697

Physical Location & Seq

Genomic Coordinates Reverse (-)

40055 .. 42883

Gene Structure 2829 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0001394.1_g000006.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

atggcatgttctagggatggggatataaccattttgattggtgattggtacacaagaaaccatacggctttgaggaagacccttgatgcagggaaaggccttggcataccagatggtgttctcatcaatgggaaaggcccttaccaatacaacactacacttgtcccggatggcattgattacgaaacaattgatgtacacccagggaaaacttaccgtcttcgcgtacacaacgttggagtgtcaactactctaaatttcaggattcagaaccacaatctgcttctagcagagtcagagggatcatatacagtacaacagaattatacgagcttagatatacatgtcggacaatcttattcatttctgttaacaacggatcagaatgcaagcagtgattattatattgtagcaagtgctagatttgtgaacgagtcacattggcaaaaagttactggagttgggattctgcgctattctaattccaaaggaaaggcagctggtcccctcccaccaccaccaagtgacgaatttgacaaaaccttctcgatgaaccaggccagatccatcagatggaatgtatcagccagtggtgcacgtcctaatccacagggatcttttagatatggctccatcaatgtaactgatgtttatgtgttgaaaaacaagccaccagtaatgatcaatgggaaacgccgagcaacactaagtggaaactcatttgtaaatcctgccacaccaatccggctagctgaccagtacaaattgaagggtgtttataagcttgacttcccaaaaaggccacttactggagcacccaggatggagacatctgtgattaatggaacatttagaggatttattgaaatcatacttcagaacaatgacaccaagatgcagagttaccacgtggatggatatgcattttttgttgttgggatggactacggtgactggacagagaatagcagggggacatataataaatgggatggaatagcccgttctacaatacaggtttatcctggggcatggacagcaatcttgatttctttggataatgttggagtctggaacattagagctgaaaatctcgattcatggtatcttggccaagaaacttatatcaaggtcgtcaatccagaaccgactaacaagactgagctccccatgccagacaatgccctattttgtggtgctctaagcaaattgcagaagcctgaagacatctcttcggaagcatcactcactattggtcaatcaagggtgttcttcactctgctgatgatcatctgcactttaattcccattttccactaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0008150 GO:0008152 GO:0009505 GO:0009506 GO:0009653 GO:0009826 GO:0009932 GO:0009987 GO:0016020 GO:0016043 GO:0016049 GO:0016491 GO:0016722 GO:0030054 GO:0030312 GO:0031090 GO:0031224 GO:0031225 GO:0031226 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0048046 GO:0048589 GO:0048856 GO:0048869 GO:0055044 GO:0055114 GO:0060560 GO:0071840 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

439

Amino Acids

48.93

Weight (kDa)

8.7

Isoelectric Point (pI)

33.44

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011697)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G12420 AT4G12420
fragaria_vesca	FvH4_5g00300
malus_domestica	MD04G1083900.v1.1 MD06G1066000.v1.1
prunus_persica	Prupe.5G075700_v2.0.a1
pyrus_communis	pycom04g07510 pycom06g05920
rosa_chinensis	RchiOBHm_Chr7g0202271
rosa_laevigata	RLG00000003613
rosa_multiflora	Rmu_co8242337.1_g000001 Rmu_sc0001394.1_g000006
rosa_roxburghii	Rroxscaffold_3G00254690
rosa_rugosa	Rorug07G0069700
rosa_samantha	Rh7AG196800 Rh7BG198000 Rh7CG208000 Rh7DG203400
rosa_wichuraiana	Rw7G017230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	780
AccB7I	CCANNNNNTGG	2 cut(s)	573, 809
AccII	CGCG	1 cut(s)	225
AclI	AACGTT	1 cut(s)	234
AclWI	GGATC	4 cut(s)	310, 387, 558, 622
AcoI	YGGCCR	1 cut(s)	1111
AcsI	RAATTY	2 cut(s)	256, 530
AcuI	CTGAAG	2 cut(s)	860, 1242
AcvI	CACGTG	1 cut(s)	910
AdeI	CACNNNGTG	2 cut(s)	524, 710
AfaI	GTAC	5 cut(s)	50, 198, 228, 315, 761
AfiI	CCNNNNNNNGG	2 cut(s)	573, 809
AflIII	ACRYGT	1 cut(s)	343
AgsI	TTSAA	3 cut(s)	661, 769, 866
AjiI	CACGTC	1 cut(s)	599
AjnI	CCWGG	4 cut(s)	202, 555, 818, 1024
AluBI	AGCT	6 cut(s)	333, 500, 752, 784, 1085, 1165
AluI	AGCT	6 cut(s)	333, 500, 752, 784, 1085, 1165
Alw21I	GWGCWC	4 cut(s)	598, 817, 1167, 1201
Alw26I	GTCTC	1 cut(s)	821
Alw44I	GTGCAC	1 cut(s)	594
AlwI	GGATC	4 cut(s)	310, 387, 558, 622
AoxI	GGCC	5 cut(s)	97, 136, 558, 800, 1111
ApaLI	GTGCAC	1 cut(s)	594
ApeKI	GCWGC	1 cut(s)	497
ApoI	RAATTY	2 cut(s)	256, 530
AseI	ATTAAT	1 cut(s)	840
AspLEI	GCGC	1 cut(s)	474
AspS9I	GGNCC	2 cut(s)	137, 503
AsuC2I	CCSGG	1 cut(s)	167
AsuHPI	GGTGA	2 cut(s)	53, 962
AsuNHI	GCTAGC	1 cut(s)	748
AvaII	GGWCC	1 cut(s)	503
BaeGI	GKGCMC	1 cut(s)	598
BalI	TGGCCA	1 cut(s)	1113
BanII	GRGCYC	1 cut(s)	1167
BbrPI	CACGTG	1 cut(s)	910
BbsI	GAAGAC	3 cut(s)	83, 212, 1230
Bbv12I	GWGCWC	4 cut(s)	598, 817, 1167, 1201
BbvI	GCAGC	1 cut(s)	509
BceAI	ACGGC	1 cut(s)	81
BciT130I	CCWGG	4 cut(s)	204, 557, 820, 1026
BclI	TGATCA	2 cut(s)	681, 1287
BcnI	CCSGG	1 cut(s)	167
BcoDI	GTCTC	1 cut(s)	821
BfaI	CTAG	4 cut(s)	12, 287, 420, 749
BisI	GCNGC	1 cut(s)	498
BlsI	GCNGC	1 cut(s)	499
Bme1390I	CCNGG	5 cut(s)	167, 204, 557, 820, 1026
Bme18I	GGWCC	1 cut(s)	503
BmgBI	CACGTC	1 cut(s)	599
BmgT120I	GGNCC	2 cut(s)	137, 503
BmiI	GGNNCC	2 cut(s)	505, 631
BmrFI	CCNGG	5 cut(s)	167, 204, 557, 820, 1026
BmsI	GCATC	3 cut(s)	76, 885, 1250
BmtI	GCTAGC	1 cut(s)	752
BpiI	GAAGAC	3 cut(s)	83, 212, 1230
BpmI	CTGGAG	2 cut(s)	477, 831
BpuMI	CCSGG	1 cut(s)	167
BsaAI	YACGTR	1 cut(s)	910
BsaBI	GATNNNNATC	1 cut(s)	1289
BsaJI	CCNNGG	5 cut(s)	100, 202, 203, 818, 1025
Bsc4I	CCNNNNNNNGG	2 cut(s)	573, 809
Bse1I	ACTGG	6 cut(s)	460, 588, 674, 757, 814, 959
Bse8I	GATNNNNATC	1 cut(s)	1289
BseBI	CCWGG	4 cut(s)	204, 557, 820, 1026
BseDI	CCNNGG	5 cut(s)	100, 202, 203, 818, 1025
BseGI	GGATG	6 cut(s)	22, 175, 828, 919, 945, 997
BseJI	GATNNNNATC	1 cut(s)	1289
BseLI	CCNNNNNNNGG	2 cut(s)	573, 809
BseMII	CTCAG	1 cut(s)	1152
BseNI	ACTGG	6 cut(s)	460, 588, 674, 757, 814, 959
BseSI	GKGCMC	1 cut(s)	598
BseXI	GCAGC	1 cut(s)	509
BsgI	GTGCAG	1 cut(s)	1279
Bsh1236I	CGCG	1 cut(s)	225
BshFI	GGCC	5 cut(s)	99, 138, 560, 802, 1113
BsiHKAI	GWGCWC	4 cut(s)	598, 817, 1167, 1201
BsiSI	CCGG	2 cut(s)	167, 745
BslFI	GGGAC	3 cut(s)	149, 489, 988
BslI	CCNNNNNNNGG	2 cut(s)	573, 809
BsmAI	GTCTC	1 cut(s)	821
BsmFI	GGGAC	3 cut(s)	149, 489, 988
BsmI	GAATGC	1 cut(s)	391
BsnI	GGCC	5 cut(s)	99, 138, 560, 802, 1113
Bsp1286I	GDGCHC	4 cut(s)	598, 817, 1167, 1201
Bsp1407I	TGTACA	1 cut(s)	196
Bsp143I	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
BspANI	GGCC	5 cut(s)	99, 138, 560, 802, 1113
BspCNI	CTCAG	1 cut(s)	1153
BspFNI	CGCG	1 cut(s)	225
BspLI	GGNNCC	2 cut(s)	505, 631
BspOI	GCTAGC	1 cut(s)	752
BspPI	GGATC	4 cut(s)	310, 387, 558, 622
BsrGI	TGTACA	1 cut(s)	196
BsrI	ACTGG	6 cut(s)	460, 588, 674, 757, 814, 959
BssECI	CCNNGG	5 cut(s)	100, 202, 203, 818, 1025
BssMI	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
BssT1I	CCWWGG	1 cut(s)	100
Bst2UI	CCWGG	4 cut(s)	204, 557, 820, 1026
Bst4CI	ACNGT	3 cut(s)	218, 313, 950
Bst6I	CTCTTC	1 cut(s)	1237
BstAUI	TGTACA	1 cut(s)	196
BstBAI	YACGTR	1 cut(s)	910
BstC8I	GCNNGC	2 cut(s)	391, 750
BstDEI	CTNAG	4 cut(s)	334, 707, 1161, 1202
BstF5I	GGATG	6 cut(s)	22, 175, 828, 919, 945, 997
BstFNI	CGCG	1 cut(s)	225
BstHHI	GCGC	1 cut(s)	474
BstKTI	GATC	6 cut(s)	305, 382, 566, 617, 684, 1290
BstMAI	GTCTC	1 cut(s)	821
BstMBI	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
BstMWI	GCNNNNNNNGC	3 cut(s)	593, 1037, 1171
BstNI	CCWGG	4 cut(s)	204, 557, 820, 1026
BstNSI	RCATGY	2 cut(s)	9, 347
BstSCI	CCNGG	5 cut(s)	165, 202, 555, 818, 1024
BstSLI	GKGCMC	1 cut(s)	598
BstUI	CGCG	1 cut(s)	225
BstV1I	GCAGC	1 cut(s)	509
BstV2I	GAAGAC	3 cut(s)	83, 212, 1230
BstX2I	RGATCY	2 cut(s)	563, 614
BstXI	CCANNNNNNTGG	1 cut(s)	914
BstYI	RGATCY	2 cut(s)	563, 614
BsuRI	GGCC	5 cut(s)	99, 138, 560, 802, 1113
BtrI	CACGTC	1 cut(s)	599
BtsCI	GGATG	6 cut(s)	22, 175, 828, 919, 945, 997
BtsI	GCAGTG	1 cut(s)	400
BtsIMutI	CAGTG	2 cut(s)	400, 595
Cac8I	GCNNGC	2 cut(s)	391, 750
CfoI	GCGC	1 cut(s)	474
Cfr13I	GGNCC	2 cut(s)	137, 503
Csp6I	GTAC	5 cut(s)	49, 197, 227, 314, 760
CviAII	CATG	5 cut(s)	6, 344, 1032, 1101, 1171
CviQI	GTAC	5 cut(s)	49, 197, 227, 314, 760
DdeI	CTNAG	4 cut(s)	334, 707, 1161, 1202
DpnI	GATC	6 cut(s)	304, 381, 565, 616, 683, 1289
DpnII	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
DraIII	CACNNNGTG	2 cut(s)	524, 710
EaeI	YGGCCR	1 cut(s)	1111
Eam1104I	CTCTTC	1 cut(s)	1237
EarI	CTCTTC	1 cut(s)	1237
Ecl136II	GAGCTC	1 cut(s)	1165
Eco130I	CCWWGG	1 cut(s)	100
Eco147I	AGGCCT	1 cut(s)	99
Eco24I	GRGCYC	1 cut(s)	1167
Eco47I	GGWCC	1 cut(s)	503
Eco53kI	GAGCTC	1 cut(s)	1165
Eco57I	CTGAAG	2 cut(s)	860, 1242
Eco72I	CACGTG	1 cut(s)	910
EcoICRI	GAGCTC	1 cut(s)	1165
EcoO109I	RGGNCCY	1 cut(s)	137
EcoRII	CCWGG	4 cut(s)	202, 555, 818, 1024
EcoT14I	CCWWGG	1 cut(s)	100
EcoT22I	ATGCAT	1 cut(s)	925
EcoT38I	GRGCYC	1 cut(s)	1167
ErhI	CCWWGG	1 cut(s)	100
FaeI	CATG	5 cut(s)	9, 347, 1035, 1104, 1174
FalI	AAGNNNNNCTT	2 cut(s)	773, 805
FaqI	GGGAC	3 cut(s)	149, 489, 988
FatI	CATG	5 cut(s)	5, 343, 1031, 1100, 1170
FbaI	TGATCA	2 cut(s)	681, 1287
Fnu4HI	GCNGC	1 cut(s)	498
FokI	GGATG	6 cut(s)	29, 182, 835, 926, 952, 1004
FriOI	GRGCYC	1 cut(s)	1167
Fsp4HI	GCNGC	1 cut(s)	498
FspBI	CTAG	4 cut(s)	12, 287, 420, 749
GlaI	GCGC	1 cut(s)	473
GluI	GCNGC	1 cut(s)	498
GsuI	CTGGAG	2 cut(s)	477, 831
HaeIII	GGCC	5 cut(s)	99, 138, 560, 802, 1113
HapII	CCGG	2 cut(s)	167, 745
HhaI	GCGC	1 cut(s)	474
Hin1II	CATG	5 cut(s)	9, 347, 1035, 1104, 1174
Hin6I	GCGC	1 cut(s)	472
HinP1I	GCGC	1 cut(s)	472
HincII	GTYRAC	2 cut(s)	246, 372
HindII	GTYRAC	2 cut(s)	246, 372
HindIII	AAGCTT	1 cut(s)	782
HinfI	GANTC	6 cut(s)	265, 293, 434, 466, 1068, 1097
HpaI	GTTAAC	1 cut(s)	372
HpaII	CCGG	2 cut(s)	167, 745
HphI	GGTGA	2 cut(s)	53, 962
Hpy166II	GTNNAC	7 cut(s)	51, 199, 229, 246, 372, 430, 596
Hpy188I	TCNGA	7 cut(s)	270, 298, 350, 384, 572, 879, 1237
Hpy188III	TCNNGA	6 cut(s)	262, 547, 1045, 1072, 1094, 1142
Hpy8I	GTNNAC	7 cut(s)	51, 199, 229, 246, 372, 430, 596
HpyAV	CCTTC	2 cut(s)	553, 763
HpyCH4III	ACNGT	3 cut(s)	218, 313, 950
HpyCH4IV	ACGT	3 cut(s)	234, 598, 909
HpyCH4V	TGCA	7 cut(s)	89, 389, 596, 898, 923, 1213, 1296
HpyF10VI	GCNNNNNNNGC	3 cut(s)	593, 1037, 1171
HpyF3I	CTNAG	4 cut(s)	334, 707, 1161, 1202
HpySE526I	ACGT	3 cut(s)	234, 598, 909
Hsp92II	CATG	5 cut(s)	9, 347, 1035, 1104, 1174
HspAI	GCGC	1 cut(s)	472
Ksp22I	TGATCA	2 cut(s)	681, 1287
KspAI	GTTAAC	1 cut(s)	372
Kzo9I	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
LmnI	GCTCC	3 cut(s)	635, 812, 1170
Lsp1109I	GCAGC	1 cut(s)	509
LweI	GCATC	3 cut(s)	76, 885, 1250
MaeI	CTAG	4 cut(s)	12, 287, 420, 749
MaeII	ACGT	3 cut(s)	234, 598, 909
MaeIII	GTNAC	6 cut(s)	435, 451, 524, 640, 902, 950
MalI	GATC	6 cut(s)	304, 381, 565, 616, 683, 1289
MboI	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
MboII	GAAGA	5 cut(s)	88, 212, 1224, 1235, 1264
MfeI	CAATTG	1 cut(s)	189
MflI	RGATCY	2 cut(s)	563, 614
MhlI	GDGCHC	4 cut(s)	598, 817, 1167, 1201
MlsI	TGGCCA	1 cut(s)	1113
MluCI	AATT	8 cut(s)	189, 256, 322, 481, 530, 764, 1208, 1302
MluNI	TGGCCA	1 cut(s)	1113
MlyI	GAGTC	3 cut(s)	302, 443, 1077
MmeI	TCCRAC	3 cut(s)	217, 328, 1045
MnlI	CCTC	4 cut(s)	66, 292, 518, 848
Mox20I	TGGCCA	1 cut(s)	1113
Mph1103I	ATGCAT	1 cut(s)	925
MscI	TGGCCA	1 cut(s)	1113
MseI	TTAA	3 cut(s)	371, 840, 1301
MslI	CAYNNNNRTG	2 cut(s)	893, 912
Msp20I	TGGCCA	1 cut(s)	1113
MspA1I	CMGCKG	1 cut(s)	500
MspI	CCGG	2 cut(s)	167, 745
MspR9I	CCNGG	5 cut(s)	167, 204, 557, 820, 1026
MunI	CAATTG	1 cut(s)	189
Mva1269I	GAATGC	1 cut(s)	391
MvaI	CCWGG	4 cut(s)	204, 557, 820, 1026
MvnI	CGCG	1 cut(s)	225
MwoI	GCNNNNNNNGC	3 cut(s)	593, 1037, 1171
NciI	CCSGG	1 cut(s)	167
NdeII	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
NheI	GCTAGC	1 cut(s)	748
NlaIII	CATG	5 cut(s)	9, 347, 1035, 1104, 1174
NlaIV	GGNNCC	2 cut(s)	505, 631
NmeAIII	GCCGAG	1 cut(s)	722
NmuCI	GTSAC	3 cut(s)	435, 524, 950
NsiI	ATGCAT	1 cut(s)	925
NspI	RCATGY	2 cut(s)	9, 347
PasI	CCCWGGG	1 cut(s)	203
PceI	AGGCCT	1 cut(s)	99
PciI	ACATGT	1 cut(s)	343
PctI	GAATGC	1 cut(s)	391
PfeI	GAWTC	3 cut(s)	265, 466, 1097
PflMI	CCANNNNNTGG	2 cut(s)	573, 809
PfoI	TCCNGGA	1 cut(s)	165
PkrI	GCNGC	1 cut(s)	499
PleI	GAGTC	3 cut(s)	301, 442, 1076
PmaCI	CACGTG	1 cut(s)	910
PmlI	CACGTG	1 cut(s)	910
PpsI	GAGTC	3 cut(s)	301, 442, 1076
Ppu21I	YACGTR	1 cut(s)	910
PscI	ACATGT	1 cut(s)	343
PshBI	ATTAAT	1 cut(s)	840
PsiI	TTATAA	1 cut(s)	780
Psp124BI	GAGCTC	1 cut(s)	1167
Psp1406I	AACGTT	1 cut(s)	234
Psp6I	CCWGG	4 cut(s)	202, 555, 818, 1024
PspCI	CACGTG	1 cut(s)	910
PspGI	CCWGG	4 cut(s)	202, 555, 818, 1024
PspN4I	GGNNCC	2 cut(s)	505, 631
PspPI	GGNCC	2 cut(s)	137, 503
PsuI	RGATCY	2 cut(s)	563, 614
PvuII	CAGCTG	1 cut(s)	500
RsaI	GTAC	5 cut(s)	50, 198, 228, 315, 761
RsaNI	GTAC	5 cut(s)	49, 197, 227, 314, 760
RseI	CAYNNNNRTG	2 cut(s)	893, 912
SacI	GAGCTC	1 cut(s)	1167
SaqAI	TTAA	3 cut(s)	371, 840, 1301
SatI	GCNGC	1 cut(s)	498
Sau3AI	GATC	6 cut(s)	302, 379, 563, 614, 681, 1287
Sau96I	GGNCC	2 cut(s)	137, 503
SchI	GAGTC	3 cut(s)	302, 443, 1077
ScrFI	CCNGG	5 cut(s)	167, 204, 557, 820, 1026
SduI	GDGCHC	4 cut(s)	598, 817, 1167, 1201
SfaNI	GCATC	3 cut(s)	76, 885, 1250
SinI	GGWCC	1 cut(s)	503
SmiMI	CAYNNNNRTG	2 cut(s)	893, 912
Sse9I	AATT	8 cut(s)	189, 256, 322, 481, 530, 764, 1208, 1302
SseBI	AGGCCT	1 cut(s)	99
SspMI	CTAG	4 cut(s)	12, 287, 420, 749
SstI	GAGCTC	1 cut(s)	1167
StuI	AGGCCT	1 cut(s)	99
StyD4I	CCNGG	5 cut(s)	165, 202, 555, 818, 1024
StyI	CCWWGG	1 cut(s)	100
TaaI	ACNGT	3 cut(s)	218, 313, 950
TaiI	ACGT	3 cut(s)	237, 601, 912
TaqI	TCGA	2 cut(s)	548, 1095
TasI	AATT	8 cut(s)	189, 256, 322, 481, 530, 764, 1208, 1302
TatI	WGTACW	3 cut(s)	196, 313, 759
TfiI	GAWTC	3 cut(s)	265, 466, 1097
Tru1I	TTAA	3 cut(s)	371, 840, 1301
Tru9I	TTAA	3 cut(s)	371, 840, 1301
TscAI	CASTG	2 cut(s)	400, 595
TseFI	GTSAC	3 cut(s)	435, 524, 950
TseI	GCWGC	1 cut(s)	497
Tsp45I	GTSAC	3 cut(s)	435, 524, 950
TspDTI	ATGAA	3 cut(s)	351, 566, 1089
TspGWI	ACGGA	1 cut(s)	392
TspRI	CASTG	2 cut(s)	400, 595
Van91I	CCANNNNNTGG	2 cut(s)	573, 809
VneI	GTGCAC	1 cut(s)	594
VpaK11BI	GGWCC	1 cut(s)	503
VspI	ATTAAT	1 cut(s)	840
XapI	RAATTY	2 cut(s)	256, 530
XceI	RCATGY	2 cut(s)	9, 347
XspI	CTAG	4 cut(s)	12, 287, 420, 749
Zsp2I	ATGCAT	1 cut(s)	925

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.