Rmu_sc0001689.1_g000010

MACPF domain-containing protein At4g24290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001689.1
OG0015525
Physical Location & Seq
Forward (+)
54470 .. 66045
11576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2865 bp
atgttcagcaggaacgacagaaattgtcgtggcccgtccccactatgtctcatctcgatccctgttaaagtgatgagatcacacaaatatgctgcaaacaagcctgcaaggaaggacgtccctatgagaggatgtaacgccaccctacatggaggtaggcatggtgccaacgccaaagagagtgacgctctggcgttatgggctatggccccagctaggatgtgtggaaggcccgtgagttcgaaggatactttggcacattccaatcttttgatcatcgacactcaaaatccatctaatgagaatcttccgggttatggtaccccctcctcttctctcacaccgacgacaaagaggagactccgcttcttcgttaaaaccaagaagcggcgagagattatggagaggagctacggagggagttcgcgtagggaggctacggagggagctcgcgtagggaggctacggagggaactcgatcttgatcctgaaaaggatcctcttcgtctgaaggatgatggcgaagatgtactagaggcagaagtgccttacttgagtacaataggcacattattgtatttagctcaatgtacaagaccggacatctcatttgcagtgaacttagagatgatggattcagatccatcatacaccaggaacgccgccaacactggtctgcgtccactatccccatcccaaaacgacataagtgttttagaaggttttgctgatgttgggtatctctctgacctatacaaagtccttgtcggagtcgccactagcagcggaatttcagatttgatcgagtggcagcgggattcgtcagagcttccgttgccgacgatctcctccgtcactagccacgcctccgcctccggttggctgagcttgaccgtctatgaaagttgtggattggccagtcgatctggtacgattggattagagctccagttccggagctgccatggcacagagagagggagagacagagagagatgtcgtcgctccccatggaaaactgaagaacaagaagaagacactgagagatcgacggagagaaaaagagagatgagtactcggaaagttgcgactgaggcggagactgcgataaaatcgatcgggctgggctacgatctcaccgttgacttgaagctcaagtactgcaagcgccagtcttccggtgttgactcgcgcttgatcgccgtcgacgacgatcaagtccgagatatcgctattcccggcgggatttgcatacccagtgtccccaagtccatcaaatgcgataagggcgagcgcatgaggttcggctccgaggtcctttcatttcaacagatgtcagagcagtttaaccaggaactggctctgtccggaaaaattccgaccggacatttcaacactgcgtttgagtttactggaggatggcaaaaggatgctgccaacacaaagactcttgctttcgacggggtttcgatcaccctctacagcgttgctcttgacaaatctcaggtagccttgcgtgatgatgtcaaacaagctgttccttcttcctgggatcctgcagcattggcaaggtttattgagaagtacggaactcatgttatagttggggtgaagatggggggaaaggatacgatatacgccaagcagatgcactcttcacctgtccaacctgctgatgtgcagaaaaaattgaaggacatggcagacaaaatgtatatagatggaactggaactggtcacgaaagagacaagttcgccaaggagcatgggcagggtattatgaacgcgattccatccagttcttacgcatctgttgagtcacaggatattaaatttgtgtgcaagagaaagggtggaagcttacaaaaaaatatacgccacagtgagtggtctcaaacagttcagcttgaacctgatgtgatctcaatgtcattggttcctatcacatcgttactgagtggagttcatgggagtggatttttgagccatgccattaacctctatcttcgttacaaaccgccaattgaagaactacatcaatttttggaatttcagcttccaaggcaatgggcaccggtgtttggtgagcttgctcttggtcctcagaagaagcaacaaagtggtgcatctttacagtttagcttcatgggccctaagctttacgttaacacaaatccggttgatgtggggaagaaaccagtgacgggtctccgactgtatttggagggaaaaaagagcaattgcttagcaatccacctgcagcatctttcaacgctgccaaagactttccaactccaggatcatttgcatatctctcatccctcatctgaccgcagatattatgagaaggttcagtggaagagcttctctcatgtctgcacagctcctattgaggctaatgatgatttctctgttgtcactggggctcacttcgaaattggagagtcggggatgaaaaaagtattatttttacgacttcatttctctaaagtcagcggtgcaaccaatgttagacaatctgagtgggacggttccccagccttgacacagaaatcgggtattatttcaaccattatcagcactcgtttctcgtctgctgcccaaaaacagccccctaatcctgctgatgtgaacataaattcggctttgtatcctggaggccccccagtacccacacagacacctaaactaatgaggtttgttgacacaacagaattgactagaggaccgcaggattcacctggatattgggttgtgtccggtgcaaggttgcttgttgagaaggctaaaatatctttacgagtcaagtattctctcctgaccatgattttacctgacgaagaagagatgcaagatggctattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

954

Amino Acids

105.57

Weight (kDa)

8.91

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 463 - 668 3.4e-38 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015525)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25760 FvH4_1g25760
malus_domestica MD15G1140100.v1.1
prunus_persica Prupe.4G285900_v2.0.a1
pyrus_communis pycom15g12660
rosa_chinensis RchiOBHm_Chr3g0484761
rosa_laevigata RLG00000023205
rosa_multiflora Rmu_sc0001689.1_g000010 Rmu_sc0006653.1_g000005
rosa_roxburghii Rroxscaffold_6G00397460
rosa_rugosa Rorug03G0213800
rosa_samantha Rh3AG264100 Rh3BG299100 Rh3CG296900 Rh3DG292700
rosa_wichuraiana Rw3G023550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2265
AasI GACNNNNNNGTC 1 cut(s) 1217
AatII GACGTC 1 cut(s) 120
Acc36I ACCTGC 2 cut(s) 1678, 2265
Acc65I GGTACC 1 cut(s) 320
AccB1I GGYRCC 3 cut(s) 164, 320, 2071
AccB7I CCANNNNNTGG 1 cut(s) 1357
AccI GTMKAC 1 cut(s) 1206
AccII CGCG 4 cut(s) 427, 453, 1192, 1787
AccIII TCCGGA 2 cut(s) 954, 1367
AclWI GGATC 8 cut(s) 52, 479, 491, 504, 635, 1547, 1560, 2307
AcoI YGGCCR 1 cut(s) 915
AcsI RAATTY 5 cut(s) 789, 1374, 1832, 2048, 2638
AcuI CTGAAG 2 cut(s) 530, 1041
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 9 cut(s) 322, 531, 559, 592, 931, 1075, 1160, 1586, 2670
AgeI ACCGGT 1 cut(s) 2074
AgsI TTSAA 8 cut(s) 1150, 1328, 1393, 1693, 1910, 2027, 2271, 2568
AjnI CCWGG 6 cut(s) 653, 1350, 1547, 2295, 2653, 2740
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
Alw21I GWGCWC 2 cut(s) 451, 948
Alw26I GTCTC 7 cut(s) 53, 352, 978, 1094, 1740, 1896, 2213
AlwI GGATC 8 cut(s) 52, 479, 491, 504, 635, 1547, 1560, 2307
AlwNI CAGNNNCTG 1 cut(s) 1357
Aor13HI TCCGGA 2 cut(s) 954, 1367
AoxI GGCC 6 cut(s) 31, 207, 230, 915, 2149, 2659
ApaI GGGCCC 1 cut(s) 2153
ApeKI GCWGC 9 cut(s) 92, 783, 811, 960, 1433, 1559, 2260, 2275, 2597
ApoI RAATTY 5 cut(s) 789, 1374, 1832, 2048, 2638
ArsI GACNNNNNNTTYG 4 cut(s) 593, 625, 750, 782
AsiGI ACCGGT 1 cut(s) 2074
Asp700I GAANNNNTTC 1 cut(s) 2363
Asp718I GGTACC 1 cut(s) 320
AspLEI GCGC 3 cut(s) 1170, 1194, 1296
AspS9I GGNCC 9 cut(s) 32, 208, 231, 1315, 2099, 2149, 2150, 2660, 2726
AsuC2I CCSGG 2 cut(s) 312, 1239
AsuHPI GGTGA 6 cut(s) 1129, 1465, 1621, 1650, 2096, 2730
AsuII TTCGAA 2 cut(s) 242, 2433
AvaII GGWCC 3 cut(s) 1315, 2099, 2726
BaeGI GKGCMC 2 cut(s) 2074, 2153
BalI TGGCCA 1 cut(s) 917
BamHI GGATCC 2 cut(s) 496, 1552
BanI GGYRCC 3 cut(s) 164, 320, 2071
BanII GRGCYC 4 cut(s) 451, 948, 2153, 2428
BarI GAAGNNNNNNTAC 2 cut(s) 1142, 1174
BbsI GAAGAC 2 cut(s) 1041, 1167
Bbv12I GWGCWC 2 cut(s) 451, 948
BbvI GCAGC 9 cut(s) 79, 795, 823, 947, 1420, 1571, 2262, 2272, 2584
BceAI ACGGC 1 cut(s) 1187
BcgI CGANNNNNNTGC 2 cut(s) 1285, 1319
BciT130I CCWGG 6 cut(s) 655, 1352, 1549, 2297, 2655, 2742
BciVI GTATCC 3 cut(s) 241, 1621, 2661
BclI TGATCA 1 cut(s) 273
BcnI CCSGG 2 cut(s) 312, 1239
BcoDI GTCTC 7 cut(s) 53, 352, 978, 1094, 1740, 1896, 2213
BfaI CTAG 5 cut(s) 216, 533, 780, 858, 2721
BfmI CTRYAG 3 cut(s) 1480, 1557, 2258
BfoI RGCGCY 1 cut(s) 1171
BfuAI ACCTGC 2 cut(s) 1678, 2265
BfuI GTATCC 3 cut(s) 241, 1621, 2661
BlpI GCTNAGC 2 cut(s) 884, 2245
BmcAI AGTACT 2 cut(s) 1075, 1160
Bme1390I CCNGG 8 cut(s) 312, 655, 1239, 1352, 1549, 2297, 2655, 2742
Bme18I GGWCC 3 cut(s) 1315, 2099, 2726
BmgT120I GGNCC 9 cut(s) 32, 208, 231, 1315, 2099, 2149, 2150, 2660, 2726
BmrFI CCNGG 8 cut(s) 312, 655, 1239, 1352, 1549, 2297, 2655, 2742
BmrI ACTGGG 3 cut(s) 1251, 2430, 2660
BmsI GCATC 6 cut(s) 1420, 1638, 1817, 2135, 2272, 2838
BmuI ACTGGG 3 cut(s) 1251, 2430, 2660
BpiI GAAGAC 2 cut(s) 1041, 1167
BplI GAGNNNNNCTC 4 cut(s) 172, 204, 2353, 2385
BpmI CTGGAG 4 cut(s) 932, 1434, 2279, 2676
Bpu10I CCTNAGC 1 cut(s) 2154
Bpu1102I GCTNAGC 2 cut(s) 884, 2245
Bpu14I TTCGAA 2 cut(s) 242, 2433
BpuEI CTTGAG 2 cut(s) 574, 1139
BpuMI CCSGG 2 cut(s) 312, 1239
Bsa29I ATCGAT 1 cut(s) 1115
BsaBI GATNNNNATC 2 cut(s) 483, 639
BsaHI GRCGYC 1 cut(s) 117
BsaI GGTCTC 2 cut(s) 1896, 2213
BsaJI CCNNGG 6 cut(s) 964, 1010, 1311, 1548, 1758, 2060
BsaWI WCCGGW 9 cut(s) 598, 875, 954, 1178, 1367, 1382, 2074, 2176, 2759
BsaXI ACNNNNNCTCC 4 cut(s) 412, 438, 442, 468
Bse118I RCCGGY 1 cut(s) 2074
Bse3DI GCAATG 1 cut(s) 2072
Bse8I GATNNNNATC 2 cut(s) 483, 639
BseAI TCCGGA 2 cut(s) 954, 1367
BseBI CCWGG 6 cut(s) 655, 1352, 1549, 2297, 2655, 2742
BseCI ATCGAT 1 cut(s) 1115
BseDI CCNNGG 6 cut(s) 964, 1010, 1311, 1548, 1758, 2060
BseGI GGATG 9 cut(s) 137, 225, 520, 692, 1424, 1435, 1793, 2317, 2457
BseJI GATNNNNATC 2 cut(s) 483, 639
BseMI GCAATG 1 cut(s) 2072
BseMII CTCAG 7 cut(s) 875, 1032, 1083, 1517, 1946, 2117, 2511
BseRI GAGGAG 4 cut(s) 319, 370, 421, 838
BseSI GKGCMC 2 cut(s) 2074, 2153
BseXI GCAGC 9 cut(s) 79, 795, 823, 947, 1420, 1571, 2262, 2272, 2584
BseYI CCCAGC 3 cut(s) 211, 1123, 2536
BsgI GTGCAG 2 cut(s) 1700, 2362
Bsh1236I CGCG 4 cut(s) 427, 453, 1192, 1787
Bsh1285I CGRYCG 2 cut(s) 1119, 1383
BshFI GGCC 6 cut(s) 33, 209, 232, 917, 2151, 2661
BshNI GGYRCC 3 cut(s) 164, 320, 2071
BshTI ACCGGT 1 cut(s) 2074
BshVI ATCGAT 1 cut(s) 1115
BsiEI CGRYCG 2 cut(s) 1119, 1383
BsiHKAI GWGCWC 2 cut(s) 451, 948
BslFI GGGAC 4 cut(s) 22, 104, 1247, 2540
BsmAI GTCTC 7 cut(s) 53, 352, 978, 1094, 1740, 1896, 2213
BsmFI GGGAC 4 cut(s) 22, 104, 1247, 2540
BsnI GGCC 6 cut(s) 33, 209, 232, 917, 2151, 2661
Bso31I GGTCTC 2 cut(s) 1896, 2213
Bsp119I TTCGAA 2 cut(s) 242, 2433
Bsp120I GGGCCC 1 cut(s) 2149
Bsp1286I GDGCHC 5 cut(s) 451, 948, 2074, 2153, 2428
Bsp13I TCCGGA 2 cut(s) 954, 1367
Bsp1407I TGTACA 1 cut(s) 590
Bsp1720I GCTNAGC 2 cut(s) 884, 2245
Bsp19I CCATGG 2 cut(s) 964, 1010
BspANI GGCC 6 cut(s) 33, 209, 232, 917, 2151, 2661
BspCNI CTCAG 7 cut(s) 876, 1033, 1084, 1516, 1947, 2116, 2512
BspDI ATCGAT 1 cut(s) 1115
BspEI TCCGGA 2 cut(s) 954, 1367
BspFNI CGCG 4 cut(s) 427, 453, 1192, 1787
BspMAI CTGCAG 2 cut(s) 1561, 2262
BspMI ACCTGC 2 cut(s) 1678, 2265
BspPI GGATC 8 cut(s) 52, 479, 491, 504, 635, 1547, 1560, 2307
BspQI GCTCTTC 1 cut(s) 2354
BspT104I TTCGAA 2 cut(s) 242, 2433
BspT107I GGYRCC 3 cut(s) 164, 320, 2071
BspTNI GGTCTC 2 cut(s) 1896, 2213
BsrDI GCAATG 1 cut(s) 2072
BsrFI RCCGGY 1 cut(s) 2074
BsrGI TGTACA 1 cut(s) 590
BssAI RCCGGY 1 cut(s) 2074
BssECI CCNNGG 6 cut(s) 964, 1010, 1311, 1548, 1758, 2060
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 4 cut(s) 964, 1010, 1758, 2060
Bst2UI CCWGG 6 cut(s) 655, 1352, 1549, 2297, 2655, 2742
Bst4CI ACNGT 7 cut(s) 895, 1141, 1883, 1900, 2136, 2217, 2531
Bst6I CTCTTC 5 cut(s) 337, 507, 1660, 2354, 2838
BstACI GRCGYC 1 cut(s) 117
BstAUI TGTACA 1 cut(s) 590
BstBI TTCGAA 2 cut(s) 242, 2433
BstC8I GCNNGC 5 cut(s) 105, 451, 1166, 1292, 2091
BstDSI CCRYGG 2 cut(s) 964, 1010
BstENI CCTNNNNNAGG 1 cut(s) 126
BstF5I GGATG 9 cut(s) 137, 225, 520, 692, 1424, 1435, 1793, 2317, 2457
BstFNI CGCG 4 cut(s) 427, 453, 1192, 1787
BstH2I RGCGCY 1 cut(s) 1171
BstHHI GCGC 3 cut(s) 1170, 1194, 1296
BstMAI GTCTC 7 cut(s) 53, 352, 978, 1094, 1740, 1896, 2213
BstMCI CGRYCG 2 cut(s) 1119, 1383
BstNI CCWGG 6 cut(s) 655, 1352, 1549, 2297, 2655, 2742
BstSCI CCNGG 8 cut(s) 310, 653, 1237, 1350, 1547, 2295, 2653, 2740
BstSFI CTRYAG 3 cut(s) 1480, 1557, 2258
BstSLI GKGCMC 2 cut(s) 2074, 2153
BstUI CGCG 4 cut(s) 427, 453, 1192, 1787
BstV1I GCAGC 9 cut(s) 79, 795, 823, 947, 1420, 1571, 2262, 2272, 2584
BstV2I GAAGAC 2 cut(s) 1041, 1167
BstX2I RGATCY 3 cut(s) 496, 640, 1552
BstXI CCANNNNNNTGG 1 cut(s) 2067
BstYI RGATCY 3 cut(s) 496, 640, 1552
Bsu15I ATCGAT 1 cut(s) 1115
BsuI GTATCC 3 cut(s) 241, 1621, 2661
BsuRI GGCC 6 cut(s) 33, 209, 232, 917, 2151, 2661
BsuTUI ATCGAT 1 cut(s) 1115
BtgI CCRYGG 2 cut(s) 964, 1010
BtsCI GGATG 9 cut(s) 137, 225, 520, 692, 1424, 1435, 1793, 2317, 2457
BtsI GCAGTG 2 cut(s) 621, 1395
BtsIMutI CAGTG 9 cut(s) 621, 669, 1038, 1264, 1395, 1888, 2205, 2360, 2418
BveI ACCTGC 2 cut(s) 1678, 2265
Cac8I GCNNGC 5 cut(s) 105, 451, 1166, 1292, 2091
CaiI CAGNNNCTG 1 cut(s) 1357
CfoI GCGC 3 cut(s) 1170, 1194, 1296
Cfr10I RCCGGY 1 cut(s) 2074
Cfr13I GGNCC 9 cut(s) 32, 208, 231, 1315, 2099, 2149, 2150, 2660, 2726
ClaI ATCGAT 1 cut(s) 1115
CseI GACGC 2 cut(s) 194, 668
Csp6I GTAC 9 cut(s) 321, 530, 558, 591, 930, 1074, 1159, 1585, 2669
CspAI ACCGGT 1 cut(s) 2074
CspCI CAANNNNNGTGG 2 cut(s) 2504, 2539
CviQI GTAC 9 cut(s) 321, 530, 558, 591, 930, 1074, 1159, 1585, 2669
DrdI GACNNNNNNGTC 1 cut(s) 1217
DseDI GACNNNNNNGTC 1 cut(s) 1217
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 5 cut(s) 337, 507, 1660, 2354, 2838
EarI CTCTTC 5 cut(s) 337, 507, 1660, 2354, 2838
EciI GGCGGA 2 cut(s) 859, 1112
Ecl136II GAGCTC 2 cut(s) 449, 946
Eco130I CCWWGG 4 cut(s) 964, 1010, 1758, 2060
Eco24I GRGCYC 4 cut(s) 451, 948, 2153, 2428
Eco31I GGTCTC 2 cut(s) 1896, 2213
Eco32I GATATC 1 cut(s) 1228
Eco47I GGWCC 3 cut(s) 1315, 2099, 2726
Eco53kI GAGCTC 2 cut(s) 449, 946
Eco57I CTGAAG 2 cut(s) 530, 1041
EcoICRI GAGCTC 2 cut(s) 449, 946
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoO109I RGGNCCY 3 cut(s) 1315, 2150, 2660
EcoRII CCWGG 6 cut(s) 653, 1350, 1547, 2295, 2653, 2740
EcoRV GATATC 1 cut(s) 1228
EcoT14I CCWWGG 4 cut(s) 964, 1010, 1758, 2060
EcoT38I GRGCYC 4 cut(s) 451, 948, 2153, 2428
ErhI CCWWGG 4 cut(s) 964, 1010, 1758, 2060
FaqI GGGAC 4 cut(s) 22, 104, 1247, 2540
FauI CCCGC 2 cut(s) 807, 1235
FbaI TGATCA 1 cut(s) 273
FblI GTMKAC 1 cut(s) 1206
FokI GGATG 9 cut(s) 144, 232, 527, 679, 1431, 1442, 1780, 2304, 2464
FriOI GRGCYC 4 cut(s) 451, 948, 2153, 2428
FspBI CTAG 5 cut(s) 216, 533, 780, 858, 2721
GlaI GCGC 3 cut(s) 1169, 1193, 1295
GsaI CCCAGC 3 cut(s) 215, 1127, 2540
GsuI CTGGAG 4 cut(s) 932, 1434, 2279, 2676
HaeII RGCGCY 1 cut(s) 1171
HaeIII GGCC 6 cut(s) 33, 209, 232, 917, 2151, 2661
HgaI GACGC 2 cut(s) 194, 668
HhaI GCGC 3 cut(s) 1170, 1194, 1296
Hin1I GRCGYC 1 cut(s) 117
Hin6I GCGC 3 cut(s) 1168, 1192, 1294
HinP1I GCGC 3 cut(s) 1168, 1192, 1294
HincII GTYRAC 5 cut(s) 1144, 1186, 1207, 2167, 2704
HindII GTYRAC 5 cut(s) 1144, 1186, 1207, 2167, 2704
HindIII AAGCTT 2 cut(s) 1858, 2156
HpaI GTTAAC 1 cut(s) 2167
HphI GGTGA 6 cut(s) 1129, 1465, 1621, 1650, 2096, 2730
Hpy166II GTNNAC 9 cut(s) 619, 683, 1144, 1186, 1207, 1410, 2167, 2632, 2704
Hpy188III TCNNGA 8 cut(s) 55, 482, 488, 955, 1368, 1493, 1739, 2818
Hpy8I GTNNAC 9 cut(s) 619, 683, 1144, 1186, 1207, 1410, 2167, 2632, 2704
Hpy99I CGWCG 8 cut(s) 349, 844, 1005, 1054, 1208, 1211, 1214, 1463
HpyAV CCTTC 9 cut(s) 106, 222, 238, 505, 713, 1551, 1687, 2341, 2776
HpyCH4III ACNGT 7 cut(s) 895, 1141, 1883, 1900, 2136, 2217, 2531
HpyCH4IV ACGT 2 cut(s) 117, 2163
HpySE526I ACGT 2 cut(s) 117, 2163
Hsp92I GRCGYC 1 cut(s) 117
HspAI GCGC 3 cut(s) 1168, 1192, 1294
Kpn2I TCCGGA 2 cut(s) 954, 1367
KpnI GGTACC 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 2167
LguI GCTCTTC 1 cut(s) 2354
LmnI GCTCC 8 cut(s) 408, 446, 951, 957, 1010, 1313, 1762, 2389
Lsp1109I GCAGC 9 cut(s) 79, 795, 823, 947, 1420, 1571, 2262, 2272, 2584
LweI GCATC 6 cut(s) 1420, 1638, 1817, 2135, 2272, 2838
MaeI CTAG 5 cut(s) 216, 533, 780, 858, 2721
MaeII ACGT 2 cut(s) 117, 2163
MaeIII GTNAC 9 cut(s) 134, 182, 853, 1736, 1818, 1950, 2009, 2200, 2416
MfeI CAATTG 2 cut(s) 2022, 2239
MflI RGATCY 3 cut(s) 496, 640, 1552
MhlI GDGCHC 5 cut(s) 451, 948, 2074, 2153, 2428
MlsI TGGCCA 1 cut(s) 917
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 7 cut(s) 354, 780, 1181, 1441, 1826, 2453, 2811
MmeI TCCRAC 5 cut(s) 748, 1403, 1690, 2236, 2314
Mox20I TGGCCA 1 cut(s) 917
MroI TCCGGA 2 cut(s) 954, 1367
MroXI GAANNNNTTC 1 cut(s) 2363
MscI TGGCCA 1 cut(s) 917
MseI TTAA 6 cut(s) 66, 375, 1347, 1830, 1995, 2166
MslI CAYNNNNRTG 2 cut(s) 87, 1971
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 3 cut(s) 786, 814, 2496
MspR9I CCNGG 8 cut(s) 312, 655, 1239, 1352, 1549, 2297, 2655, 2742
MunI CAATTG 2 cut(s) 2022, 2239
MvaI CCWGG 6 cut(s) 655, 1352, 1549, 2297, 2655, 2742
MvnI CGCG 4 cut(s) 427, 453, 1192, 1787
NciI CCSGG 2 cut(s) 312, 1239
NcoI CCATGG 2 cut(s) 964, 1010
NmuCI GTSAC 6 cut(s) 182, 853, 1736, 1818, 2200, 2416
NspV TTCGAA 2 cut(s) 242, 2433
PaqCI CACCTGC 1 cut(s) 2265
PciSI GCTCTTC 1 cut(s) 2354
PcsI WCGNNNNNNNCGW 2 cut(s) 1137, 1206
PdmI GAANNNNTTC 1 cut(s) 2363
PfeI GAWTC 5 cut(s) 304, 635, 818, 1789, 2735
PflMI CCANNNNNTGG 1 cut(s) 1357
PfoI TCCNGGA 2 cut(s) 2295, 2653
PinAI ACCGGT 1 cut(s) 2074
Ple19I CGATCG 1 cut(s) 1119
PleI GAGTC 7 cut(s) 354, 779, 1181, 1441, 1825, 2452, 2810
PpsI GAGTC 7 cut(s) 354, 779, 1181, 1441, 1825, 2452, 2810
PpuMI RGGWCCY 1 cut(s) 1315
Psp124BI GAGCTC 2 cut(s) 451, 948
Psp5II RGGWCCY 1 cut(s) 1315
Psp6I CCWGG 6 cut(s) 653, 1350, 1547, 2295, 2653, 2740
PspFI CCCAGC 3 cut(s) 211, 1123, 2536
PspGI CCWGG 6 cut(s) 653, 1350, 1547, 2295, 2653, 2740
PspOMI GGGCCC 1 cut(s) 2149
PspPI GGNCC 9 cut(s) 32, 208, 231, 1315, 2099, 2149, 2150, 2660, 2726
PspPPI RGGWCCY 1 cut(s) 1315
PstI CTGCAG 2 cut(s) 1561, 2262
PstNI CAGNNNCTG 1 cut(s) 1357
PsuI RGATCY 3 cut(s) 496, 640, 1552
PvuI CGATCG 1 cut(s) 1119
RsaI GTAC 9 cut(s) 322, 531, 559, 592, 931, 1075, 1160, 1586, 2670
RsaNI GTAC 9 cut(s) 321, 530, 558, 591, 930, 1074, 1159, 1585, 2669
RseI CAYNNNNRTG 2 cut(s) 87, 1971
SacI GAGCTC 2 cut(s) 451, 948
SalI GTCGAC 1 cut(s) 1205
SapI GCTCTTC 1 cut(s) 2354
SaqAI TTAA 6 cut(s) 66, 375, 1347, 1830, 1995, 2166
Sau96I GGNCC 9 cut(s) 32, 208, 231, 1315, 2099, 2149, 2150, 2660, 2726
ScaI AGTACT 2 cut(s) 1075, 1160
SchI GAGTC 7 cut(s) 354, 780, 1181, 1441, 1826, 2453, 2811
ScrFI CCNGG 8 cut(s) 312, 655, 1239, 1352, 1549, 2297, 2655, 2742
SduI GDGCHC 5 cut(s) 451, 948, 2074, 2153, 2428
SfaNI GCATC 6 cut(s) 1420, 1638, 1817, 2135, 2272, 2838
SfcI CTRYAG 3 cut(s) 1480, 1557, 2258
SfuI TTCGAA 2 cut(s) 242, 2433
SgrAI CRCCGGYG 1 cut(s) 2074
SgrDI CGTCGACG 1 cut(s) 1205
SinI GGWCC 3 cut(s) 1315, 2099, 2726
SmiMI CAYNNNNRTG 2 cut(s) 87, 1971
SmlI CTYRAG 2 cut(s) 553, 1154
SmoI CTYRAG 2 cut(s) 553, 1154
SspMI CTAG 5 cut(s) 216, 533, 780, 858, 2721
SstI GAGCTC 2 cut(s) 451, 948
StyD4I CCNGG 8 cut(s) 310, 653, 1237, 1350, 1547, 2295, 2653, 2740
StyI CCWWGG 4 cut(s) 964, 1010, 1758, 2060
TaaI ACNGT 7 cut(s) 895, 1141, 1883, 1900, 2136, 2217, 2531
TaiI ACGT 2 cut(s) 120, 2166
TatI WGTACW 5 cut(s) 529, 557, 590, 1073, 1158
TauI GCSGC 2 cut(s) 391, 665
TfiI GAWTC 5 cut(s) 304, 635, 818, 1789, 2735
Tru1I TTAA 6 cut(s) 66, 375, 1347, 1830, 1995, 2166
Tru9I TTAA 6 cut(s) 66, 375, 1347, 1830, 1995, 2166
TscAI CASTG 9 cut(s) 621, 676, 1045, 1264, 1402, 1888, 2205, 2360, 2425
TseFI GTSAC 6 cut(s) 182, 853, 1736, 1818, 2200, 2416
TseI GCWGC 9 cut(s) 92, 783, 811, 960, 1433, 1559, 2260, 2275, 2597
Tsp45I GTSAC 6 cut(s) 182, 853, 1736, 1818, 2200, 2416
TspDTI ATGAA 7 cut(s) 915, 1311, 1796, 1955, 2134, 2468, 2468
TspGWI ACGGA 7 cut(s) 429, 455, 481, 822, 841, 1067, 1602
TspRI CASTG 9 cut(s) 621, 676, 1045, 1264, 1402, 1888, 2205, 2360, 2425
Van91I CCANNNNNTGG 1 cut(s) 1357
VpaK11BI GGWCC 3 cut(s) 1315, 2099, 2726
XagI CCTNNNNNAGG 1 cut(s) 126
XapI RAATTY 5 cut(s) 789, 1374, 1832, 2048, 2638
XmiI GTMKAC 1 cut(s) 1206
XmnI GAANNNNTTC 1 cut(s) 2363
XspI CTAG 5 cut(s) 216, 533, 780, 858, 2721
ZraI GACGTC 1 cut(s) 118
ZrmI AGTACT 2 cut(s) 1075, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.