Rmu_sc0001777.1_g000004

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001777.1
OG0020674
Physical Location & Seq
Reverse (-)
14895 .. 17540
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001777.1_g000004.1.cds

Sequence Viewer

Length: 1083 bp
atgggtggctggagcatgcgcggctggtcgctggcaggaaggaagcagcagcaccggcgcaggggtgctgcaggtcgtgcagactgggcaggggcagattgctgcaggtcgtgcggcagatcgttaggctgcgagctggtgtgcagcacgggctgcagggaggcgctggagggctgctgctgggagctgggtgcgcatgaggcaagctttatcaggaaaattgttgaccatattactggaaagcttcagagtactgaacacttgcagatagctatgcacccaattggactaacttctcgtgtggaatatataaactatgggctagggaaactacaaagcaaaccaatggccggtgaggttcttttgcaagaacaacttctttctcatatcttgagaacaaccaagataaaggtagatagtgttcatagagggatcaatgtgatcaagaaaaagcttagaggtttaaggttgctaaggcaactgagagtggatgcaatctatttgacagaagcaatgaatgaagatgaagctcttgagctctttagttggcatgcttttggtaatagctgtcctgatgaagaatatttagaactctcaagaagtgttgttacttattgtggggaaatagaaatcagagtccttattcaacgttgtcttgtagctgttgatggaggaaacaagctgatgatgcatgatttgcttcgagacatggatcccagcgatcccatcgctgctgcccagccgagcccggcctcacctcccttgtccagcgctgcggactccagccagccccacctccgcaacgacagcagccccacagatcggcctctccagaccggctctgtccagatcgaggaacctcaaacccacaccggctccgtccatatcagcagcaacccatctgcacaccgactcctccctcgcatctgcaacgacgctgttgcgtcggtctcctcctctcctgcgcagcccagccccgcgcccttcgacccctcgtctgtgcagatccaggcgccggctcaccacaaacctgcagcgcagcctcgcctagctgcctcagacggaacagaaacagaaaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

360

Amino Acids

39.26

Weight (kDa)

6.94

Isoelectric Point (pI)

65.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020674)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019178
rosa_multiflora Rmu_sc0001777.1_g000004
rosa_roxburghii Rroxscaffold_3G00237170
rosa_samantha Rh2AG431200 Rh7DG397800
rosa_wichuraiana Rw2G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 195, 966
Acc36I ACCTGC 3 cut(s) 62, 96, 1039
AccB1I GGYRCC 1 cut(s) 1012
AccII CGCG 2 cut(s) 21, 980
AciI CCGC 5 cut(s) 21, 114, 776, 799, 978
AclI AACGTT 1 cut(s) 649
AclWI GGATC 5 cut(s) 440, 707, 716, 720, 1000
AcoI YGGCCR 1 cut(s) 348
AcuI CTGAAG 1 cut(s) 230
AcyI GRCGYC 1 cut(s) 1013
AfaI GTAC 1 cut(s) 253
AfeI AGCGCT 1 cut(s) 772
AfiI CCNNNNNNNGG 5 cut(s) 61, 350, 822, 853, 1015
AgsI TTSAA 1 cut(s) 647
AhdI GACNNNNNGTC 1 cut(s) 994
AjnI CCWGG 1 cut(s) 1008
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 2 cut(s) 699, 955
AlwI GGATC 5 cut(s) 440, 707, 716, 720, 1000
Aor51HI AGCGCT 1 cut(s) 772
AoxI GGCC 3 cut(s) 348, 750, 824
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 9 cut(s) 21, 60, 166, 196, 773, 967, 982, 1015, 1039
AsuC2I CCSGG 1 cut(s) 749
AsuHPI GGTGA 3 cut(s) 365, 747, 1013
BamHI GGATCC 1 cut(s) 712
BanI GGYRCC 1 cut(s) 1012
BanII GRGCYC 2 cut(s) 540, 749
BarI GAAGNNNNNNTAC 2 cut(s) 592, 624
BauI CACGAG 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 540
BccI CCATC 3 cut(s) 662, 734, 907
BcgI CGANNNNNNTGC 2 cut(s) 923, 957
BciT130I CCWGG 1 cut(s) 1010
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 1 cut(s) 749
BcoDI GTCTC 2 cut(s) 699, 955
BfaI CTAG 2 cut(s) 323, 1049
BfmI CTRYAG 4 cut(s) 69, 103, 154, 1032
BfoI RGCGCY 3 cut(s) 167, 774, 1016
BfuAI ACCTGC 3 cut(s) 62, 96, 1039
BmcAI AGTACT 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 749, 1010
BmeRI GACNNNNNGTC 1 cut(s) 994
BmiI GGNNCC 4 cut(s) 714, 858, 877, 1014
BmrFI CCNGG 2 cut(s) 749, 1010
BmrI ACTGGG 1 cut(s) 94
BmsI GCATC 3 cut(s) 481, 678, 933
BmuI ACTGGG 1 cut(s) 94
BpmI CTGGAG 4 cut(s) 31, 188, 766, 815
Bpu10I CCTNAGC 1 cut(s) 473
BpuEI CTTGAG 3 cut(s) 412, 554, 580
BpuMI CCSGG 1 cut(s) 749
BsaHI GRCGYC 1 cut(s) 1013
BsaI GGTCTC 1 cut(s) 955
BsaXI ACNNNNNCTCC 4 cut(s) 860, 890, 897, 927
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 350, 822, 853, 1015
Bse118I RCCGGY 5 cut(s) 54, 350, 836, 872, 1015
Bse1I ACTGG 2 cut(s) 89, 241
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 1010
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 5 cut(s) 61, 350, 822, 853, 1015
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 473, 1071
BseNI ACTGG 2 cut(s) 89, 241
BseRI GAGGAG 3 cut(s) 905, 943, 946
BseYI CCCAGC 5 cut(s) 180, 187, 716, 738, 971
BsgI GTGCAG 4 cut(s) 99, 163, 888, 1022
Bsh1236I CGCG 2 cut(s) 21, 980
BshFI GGCC 3 cut(s) 350, 752, 826
BshNI GGYRCC 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 540
BsiSI CCGG 6 cut(s) 55, 351, 749, 837, 873, 1016
BslI CCNNNNNNNGG 5 cut(s) 61, 350, 822, 853, 1015
BsmAI GTCTC 2 cut(s) 699, 955
BsnI GGCC 3 cut(s) 350, 752, 826
Bso31I GGTCTC 1 cut(s) 955
Bsp1286I GDGCHC 2 cut(s) 540, 749
Bsp143I GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
BspACI CCGC 5 cut(s) 21, 114, 776, 799, 978
BspANI GGCC 3 cut(s) 350, 752, 826
BspCNI CTCAG 2 cut(s) 474, 1070
BspFNI CGCG 2 cut(s) 21, 980
BspLI GGNNCC 4 cut(s) 714, 858, 877, 1014
BspMAI CTGCAG 4 cut(s) 73, 107, 158, 1036
BspMI ACCTGC 3 cut(s) 62, 96, 1039
BspPI GGATC 5 cut(s) 440, 707, 716, 720, 1000
BspT107I GGYRCC 1 cut(s) 1012
BspTNI GGTCTC 1 cut(s) 955
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 5 cut(s) 54, 350, 836, 872, 1015
BsrI ACTGG 2 cut(s) 89, 241
BssAI RCCGGY 5 cut(s) 54, 350, 836, 872, 1015
BssMI GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
BssNI GRCGYC 1 cut(s) 1013
BssSI CACGAG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 1010
BstACI GRCGYC 1 cut(s) 1013
BstAPI GCANNNNNTGC 4 cut(s) 77, 111, 153, 697
BstC8I GCNNGC 7 cut(s) 17, 33, 134, 205, 552, 788, 1017
BstDEI CTNAG 4 cut(s) 455, 473, 482, 1057
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 2 cut(s) 21, 980
BstH2I RGCGCY 3 cut(s) 167, 774, 1016
BstHHI GCGC 9 cut(s) 21, 60, 166, 196, 773, 967, 982, 1015, 1039
BstKTI GATC 8 cut(s) 122, 435, 444, 715, 724, 823, 852, 1008
BstMAI GTCTC 2 cut(s) 699, 955
BstMBI GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
BstNI CCWGG 1 cut(s) 1010
BstNSI RCATGY 2 cut(s) 19, 554
BstSCI CCNGG 2 cut(s) 747, 1008
BstSFI CTRYAG 4 cut(s) 69, 103, 154, 1032
BstUI CGCG 2 cut(s) 21, 980
BstX2I RGATCY 2 cut(s) 712, 1005
BstXI CCANNNNNNTGG 1 cut(s) 236
BstYI RGATCY 2 cut(s) 712, 1005
BsuRI GGCC 3 cut(s) 350, 752, 826
BtgZI GCGATG 1 cut(s) 712
BtsCI GGATG 1 cut(s) 496
BveI ACCTGC 3 cut(s) 62, 96, 1039
Cac8I GCNNGC 7 cut(s) 17, 33, 134, 205, 552, 788, 1017
CfoI GCGC 9 cut(s) 21, 60, 166, 196, 773, 967, 982, 1015, 1039
Cfr10I RCCGGY 5 cut(s) 54, 350, 836, 872, 1015
CseI GACGC 2 cut(s) 933, 944
Csp6I GTAC 1 cut(s) 252
CviAII CATG 5 cut(s) 16, 197, 551, 692, 709
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 4 cut(s) 455, 473, 482, 1057
DinI GGCGCC 1 cut(s) 1014
DpnI GATC 8 cut(s) 121, 434, 443, 714, 723, 822, 851, 1007
DpnII GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
DriI GACNNNNNGTC 1 cut(s) 994
EaeI YGGCCR 1 cut(s) 348
Eam1105I GACNNNNNGTC 1 cut(s) 994
Ecl136II GAGCTC 1 cut(s) 538
Eco24I GRGCYC 2 cut(s) 540, 749
Eco31I GGTCTC 1 cut(s) 955
Eco47III AGCGCT 1 cut(s) 772
Eco53kI GAGCTC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 1008
EcoT22I ATGCAT 1 cut(s) 693
EcoT38I GRGCYC 2 cut(s) 540, 749
EgeI GGCGCC 1 cut(s) 1014
EheI GGCGCC 1 cut(s) 1014
FaeI CATG 5 cut(s) 19, 200, 554, 695, 712
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 5 cut(s) 15, 196, 550, 691, 708
FauI CCCGC 1 cut(s) 985
FbaI TGATCA 1 cut(s) 441
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 2 cut(s) 540, 749
FspAI RTGCGCAY 1 cut(s) 195
FspBI CTAG 2 cut(s) 323, 1049
FspI TGCGCA 2 cut(s) 195, 966
GlaI GCGC 9 cut(s) 20, 59, 165, 195, 772, 966, 981, 1014, 1038
GsaI CCCAGC 5 cut(s) 184, 191, 720, 742, 975
GsuI CTGGAG 4 cut(s) 31, 188, 766, 815
HaeII RGCGCY 3 cut(s) 167, 774, 1016
HaeIII GGCC 3 cut(s) 350, 752, 826
HapII CCGG 6 cut(s) 55, 351, 749, 837, 873, 1016
HgaI GACGC 2 cut(s) 933, 944
HhaI GCGC 9 cut(s) 21, 60, 166, 196, 773, 967, 982, 1015, 1039
Hin1I GRCGYC 1 cut(s) 1013
Hin1II CATG 5 cut(s) 19, 200, 554, 695, 712
Hin6I GCGC 9 cut(s) 19, 58, 164, 194, 771, 965, 980, 1013, 1037
HinP1I GCGC 9 cut(s) 19, 58, 164, 194, 771, 965, 980, 1013, 1037
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 3 cut(s) 205, 242, 452
HinfI GANTC 3 cut(s) 636, 779, 912
HpaII CCGG 6 cut(s) 55, 351, 749, 837, 873, 1016
HphI GGTGA 3 cut(s) 365, 747, 1013
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 3 cut(s) 249, 635, 1060
Hpy188III TCNNGA 9 cut(s) 214, 391, 445, 533, 572, 597, 704, 832, 847
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 2 cut(s) 938, 949
HpyAV CCTTC 2 cut(s) 33, 994
HpyCH4IV ACGT 1 cut(s) 649
HpyF3I CTNAG 4 cut(s) 455, 473, 482, 1057
HpySE526I ACGT 1 cut(s) 649
Hsp92I GRCGYC 1 cut(s) 1013
Hsp92II CATG 5 cut(s) 19, 200, 554, 695, 712
HspAI GCGC 9 cut(s) 19, 58, 164, 194, 771, 965, 980, 1013, 1037
KasI GGCGCC 1 cut(s) 1012
KroI GCCGGC 1 cut(s) 1015
KroNI GCCGGC 1 cut(s) 1017
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
LmnI GCTCC 3 cut(s) 12, 184, 881
LweI GCATC 3 cut(s) 481, 678, 933
MaeI CTAG 2 cut(s) 323, 1049
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 1 cut(s) 607
MalI GATC 8 cut(s) 121, 434, 443, 714, 723, 822, 851, 1007
MboI GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
MboII GAAGA 2 cut(s) 533, 590
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 2 cut(s) 712, 1005
MhlI GDGCHC 2 cut(s) 540, 749
MluCI AATT 2 cut(s) 219, 282
Mly113I GGCGCC 1 cut(s) 1013
MlyI GAGTC 3 cut(s) 645, 773, 906
Mph1103I ATGCAT 1 cut(s) 693
MroNI GCCGGC 1 cut(s) 1015
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 1 cut(s) 464
MspI CCGG 6 cut(s) 55, 351, 749, 837, 873, 1016
MspR9I CCNGG 2 cut(s) 749, 1010
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 1010
MvnI CGCG 2 cut(s) 21, 980
NaeI GCCGGC 1 cut(s) 1017
NarI GGCGCC 1 cut(s) 1013
NciI CCSGG 1 cut(s) 749
NdeII GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
NgoMIV GCCGGC 1 cut(s) 1015
NlaIII CATG 5 cut(s) 19, 200, 554, 695, 712
NlaIV GGNNCC 4 cut(s) 714, 858, 877, 1014
NmeAIII GCCGAG 1 cut(s) 768
NsbI TGCGCA 2 cut(s) 195, 966
NsiI ATGCAT 1 cut(s) 693
NspI RCATGY 2 cut(s) 19, 554
PaeI GCATGC 2 cut(s) 19, 554
PdiI GCCGGC 1 cut(s) 1017
PdmI GAANNNNTTC 1 cut(s) 375
PleI GAGTC 3 cut(s) 644, 773, 906
PluTI GGCGCC 1 cut(s) 1016
PpsI GAGTC 3 cut(s) 644, 773, 906
Psp124BI GAGCTC 1 cut(s) 540
Psp1406I AACGTT 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 1008
PspFI CCCAGC 5 cut(s) 180, 187, 716, 738, 971
PspGI CCWGG 1 cut(s) 1008
PspN4I GGNNCC 4 cut(s) 714, 858, 877, 1014
PsrI GAACNNNNNNTAC 2 cut(s) 405, 437
PstI CTGCAG 4 cut(s) 73, 107, 158, 1036
PsuI RGATCY 2 cut(s) 712, 1005
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
SacI GAGCTC 1 cut(s) 540
SaqAI TTAA 1 cut(s) 464
Sau3AI GATC 8 cut(s) 119, 432, 441, 712, 721, 820, 849, 1005
ScaI AGTACT 1 cut(s) 253
SchI GAGTC 3 cut(s) 645, 773, 906
ScrFI CCNGG 2 cut(s) 749, 1010
SduI GDGCHC 2 cut(s) 540, 749
SfaNI GCATC 3 cut(s) 481, 678, 933
SfcI CTRYAG 4 cut(s) 69, 103, 154, 1032
SfoI GGCGCC 1 cut(s) 1014
SgrAI CRCCGGYG 1 cut(s) 54
SmlI CTYRAG 3 cut(s) 391, 533, 595
SmoI CTYRAG 3 cut(s) 391, 533, 595
SphI GCATGC 2 cut(s) 19, 554
Sse9I AATT 2 cut(s) 219, 282
SsiI CCGC 5 cut(s) 21, 114, 776, 799, 978
SspDI GGCGCC 1 cut(s) 1012
SspI AATATT 1 cut(s) 584
SspMI CTAG 2 cut(s) 323, 1049
SstI GAGCTC 1 cut(s) 540
StyD4I CCNGG 2 cut(s) 747, 1008
TaiI ACGT 1 cut(s) 652
TaqI TCGA 3 cut(s) 703, 852, 987
TaqII GACCGA 1 cut(s) 937
TasI AATT 2 cut(s) 219, 282
TatI WGTACW 1 cut(s) 251
TauI GCSGC 2 cut(s) 24, 117
Tru1I TTAA 1 cut(s) 464
Tru9I TTAA 1 cut(s) 464
TspDTI ATGAA 5 cut(s) 413, 530, 534, 540, 591
TspGWI ACGGA 2 cut(s) 868, 1077
XceI RCATGY 2 cut(s) 19, 554
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 2 cut(s) 323, 1049
ZrmI AGTACT 1 cut(s) 253
Zsp2I ATGCAT 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.