Rh2AG431200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0020674
Physical Location & Seq
Reverse (-)
64009709 .. 64020383
10675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG431200.1

Sequence Viewer

Length: 333 bp
ATGACGTTGGCGAGGAGCGTCGCGATGTGTAAGCATCGGAAAGGCAGGTTATCACCAATGGCAGCCACGGTCCGGTTCTCTATGTCGGCGACGAGAGATCGGAAATCGGGGATTGAAGAAGAAGCAGTCACGAGAAAATGTAAAGTTGAGATAAGGGGCGCTGGTCGTGCGGATTGGGCAGGGGCGCTGGTCGTGCGGCAGGGGCGCTGCAGGTCGTGCGGCAGATGGCTGGGCTGCAGTAGGATGCCTGTGAGCGGAGGAGCAGGGCGAGCAGCAGGGGCGTGCTGGGCTGCGGGCTGGATAGCTTGCATCCAACATATTCTTATGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

110

Amino Acids

11.88

Weight (kDa)

11.04

Isoelectric Point (pI)

62.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020674)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019178
rosa_multiflora Rmu_sc0001777.1_g000004
rosa_roxburghii Rroxscaffold_3G00237170
rosa_samantha Rh2AG431200 Rh7DG397800
rosa_wichuraiana Rw2G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 36, 201
AccBSI CCGCTC 1 cut(s) 255
AccII CGCG 1 cut(s) 23
AciI CCGC 5 cut(s) 170, 196, 219, 255, 293
AfiI CCNNNNNNNGG 2 cut(s) 72, 254
AgsI TTSAA 1 cut(s) 116
AluBI AGCT 1 cut(s) 305
AluI AGCT 1 cut(s) 305
ApeKI GCWGC 5 cut(s) 62, 207, 234, 272, 290
AspLEI GCGC 3 cut(s) 161, 187, 207
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 45
AvaII GGWCC 1 cut(s) 70
BauI CACGAG 1 cut(s) 130
BbvI GCAGC 5 cut(s) 74, 194, 221, 277, 284
BccI CCATC 1 cut(s) 219
BfmI CTRYAG 2 cut(s) 208, 235
BfoI RGCGCY 3 cut(s) 162, 188, 208
BfuAI ACCTGC 2 cut(s) 36, 201
BisI GCNGC 7 cut(s) 63, 197, 208, 220, 235, 273, 291
BlsI GCNGC 7 cut(s) 64, 198, 209, 221, 236, 274, 292
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmsI GCATC 3 cut(s) 43, 234, 318
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 254
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 2 cut(s) 249, 309
BseLI CCNNNNNNNGG 2 cut(s) 72, 254
BseRI GAGGAG 2 cut(s) 28, 273
BseXI GCAGC 5 cut(s) 74, 194, 221, 277, 284
BseYI CCCAGC 2 cut(s) 229, 285
Bsh1236I CGCG 1 cut(s) 23
BsiSI CCGG 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 72, 254
Bsp143I GATC 1 cut(s) 97
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 5 cut(s) 170, 196, 219, 255, 293
BspFNI CGCG 1 cut(s) 23
BspMAI CTGCAG 2 cut(s) 212, 239
BspMI ACCTGC 2 cut(s) 36, 201
BsrBI CCGCTC 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 97
BssSI CACGAG 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 130
Bst4CI ACNGT 1 cut(s) 70
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 4 cut(s) 270, 283, 295, 307
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 249, 309
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 3 cut(s) 162, 188, 208
BstHHI GCGC 3 cut(s) 161, 187, 207
BstKTI GATC 1 cut(s) 100
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 8 cut(s) 167, 176, 193, 202, 216, 269, 278, 287
BstSFI CTRYAG 2 cut(s) 208, 235
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 5 cut(s) 74, 194, 221, 277, 284
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 38
BtsCI GGATG 2 cut(s) 249, 309
BtuMI TCGCGA 1 cut(s) 23
BveI ACCTGC 2 cut(s) 36, 201
Cac8I GCNNGC 4 cut(s) 270, 283, 295, 307
CfoI GCGC 3 cut(s) 161, 187, 207
Cfr13I GGNCC 1 cut(s) 70
CpoI CGGWCCG 1 cut(s) 70
CseI GACGC 1 cut(s) 7
CspI CGGWCCG 1 cut(s) 70
CviJI RGCY 6 cut(s) 65, 229, 234, 290, 297, 305
CviKI_1 RGCY 6 cut(s) 65, 229, 234, 290, 297, 305
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 70
FaiI YATR 3 cut(s) 83, 318, 326
FauI CCCGC 1 cut(s) 286
Fnu4HI GCNGC 7 cut(s) 63, 197, 208, 220, 235, 273, 291
FokI GGATG 2 cut(s) 256, 296
Fsp4HI GCNGC 7 cut(s) 63, 197, 208, 220, 235, 273, 291
GlaI GCGC 3 cut(s) 160, 186, 206
GluI GCNGC 7 cut(s) 63, 197, 208, 220, 235, 273, 291
GsaI CCCAGC 2 cut(s) 233, 289
HaeII RGCGCY 3 cut(s) 162, 188, 208
HapII CCGG 1 cut(s) 73
HgaI GACGC 1 cut(s) 7
HhaI GCGC 3 cut(s) 161, 187, 207
Hin6I GCGC 3 cut(s) 159, 185, 205
HinP1I GCGC 3 cut(s) 159, 185, 205
HpaII CCGG 1 cut(s) 73
HphI GGTGA 1 cut(s) 45
Hpy188I TCNGA 2 cut(s) 39, 102
Hpy188III TCNNGA 2 cut(s) 22, 130
Hpy99I CGWCG 2 cut(s) 23, 94
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 4 cut(s) 210, 237, 309, 328
HpyF10VI GCNNNNNNNGC 8 cut(s) 167, 176, 193, 202, 216, 269, 278, 287
HpySE526I ACGT 1 cut(s) 5
HspAI GCGC 3 cut(s) 159, 185, 205
Kzo9I GATC 1 cut(s) 97
LmnI GCTCC 2 cut(s) 15, 260
Lsp1109I GCAGC 5 cut(s) 74, 194, 221, 277, 284
LweI GCATC 3 cut(s) 43, 234, 318
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 127
MalI GATC 1 cut(s) 99
MbiI CCGCTC 1 cut(s) 255
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 128, 131
MnlI CCTC 2 cut(s) 6, 251
MspI CCGG 1 cut(s) 73
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 8 cut(s) 167, 176, 193, 202, 216, 269, 278, 287
NdeII GATC 1 cut(s) 97
NmuCI GTSAC 1 cut(s) 127
NruI TCGCGA 1 cut(s) 23
PkrI GCNGC 7 cut(s) 64, 198, 209, 221, 236, 274, 292
PspFI CCCAGC 2 cut(s) 229, 285
PspPI GGNCC 1 cut(s) 70
PstI CTGCAG 2 cut(s) 212, 239
RruI TCGCGA 1 cut(s) 23
Rsr2I CGGWCCG 1 cut(s) 70
RsrII CGGWCCG 1 cut(s) 70
SatI GCNGC 7 cut(s) 63, 197, 208, 220, 235, 273, 291
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 1 cut(s) 70
SetI ASST 4 cut(s) 8, 50, 215, 307
SfaNI GCATC 3 cut(s) 43, 234, 318
SfcI CTRYAG 2 cut(s) 208, 235
SinI GGWCC 1 cut(s) 70
SsiI CCGC 5 cut(s) 170, 196, 219, 255, 293
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 1 cut(s) 8
TauI GCSGC 2 cut(s) 199, 222
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 5 cut(s) 62, 207, 234, 272, 290
Tsp45I GTSAC 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.