Rw2G035230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0020674
Physical Location & Seq
Reverse (-)
58087453 .. 58090469
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G035230.1

Sequence Viewer

Length: 630 bp
ATGCTTCTGGGCGATGATGGTGGCGAGGAACGTAGCGATGTGAGCTCGTGTAAGCATCGGAAAGGCAGGTTTTCACCAATGGCAGCCACGGTCCGGTTCTCTATGTCGGTGACGAGAGATTGGAAATCAGGATTGAAGAAGAAGCAGTCACGAGCGATTGCTGCATTATTTGGAGTTCCAATTCTAGGTCAAAGTATACTTTTTGGTGCAATAGGAAATAATCCCAGATTAACTTTAGTACATCATGCCCTTACTCTGCCCCTCTACTTGACTCACAGGAAGTTTGAGCTTTTTGTGAGTTGTGGTGGAAATACTCCTGGGGTGCGATTTGCGCAAGCAAGTGCTAAGAAGGCTATTGGTTTCGCTGAGGTTACAATTTTGGCTGGTGTTCTCCTTCACTGTTCTATTTCACCCAAAGGACTTCCAATTTTGTTAATAAGTGTGTTTGTGGTTTGCCTATGCATGACTACAATGAAGGGACAATTGATTGCTTTCTGGTATCTTGGGGTTGGAGTGACGCATATCCCATGCTACGGCGTCTCTGCTTTAATTGTTAGAATGAAGCAGTTGTGGTTCTCAAGAACATTCCTGGAGTCTTTTCAGGGTGAGAGGTGTCGTTGCGTGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.91

Weight (kDa)

9.91

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020674)

Species Orthologous Gene IDs
rosa_laevigata RLG00000019178
rosa_multiflora Rmu_sc0001777.1_g000004
rosa_roxburghii Rroxscaffold_3G00237170
rosa_samantha Rh2AG431200 Rh7DG397800
rosa_wichuraiana Rw2G035230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 333
Acc36I ACCTGC 1 cut(s) 57
AccI GTMKAC 1 cut(s) 196
AcyI GRCGYC 1 cut(s) 537
AfaI GTAC 1 cut(s) 240
AfiI CCNNNNNNNGG 3 cut(s) 93, 185, 533
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 2 cut(s) 316, 588
AluBI AGCT 2 cut(s) 45, 289
AluI AGCT 2 cut(s) 45, 289
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 544
ApeKI GCWGC 2 cut(s) 83, 161
AspLEI GCGC 1 cut(s) 334
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 4 cut(s) 66, 121, 402, 617
AvaII GGWCC 1 cut(s) 91
BanII GRGCYC 1 cut(s) 47
BauI CACGAG 2 cut(s) 46, 150
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 2 cut(s) 95, 148
BccI CCATC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 550
BciT130I CCWGG 2 cut(s) 318, 590
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 84, 162
BlsI GCNGC 2 cut(s) 85, 163
Bme1390I CCNGG 2 cut(s) 318, 590
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmrFI CCNGG 2 cut(s) 318, 590
BmsI GCATC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 562
BsaHI GRCGYC 1 cut(s) 537
BsaJI CCNNGG 2 cut(s) 87, 317
BsaWI WCCGGW 1 cut(s) 93
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 185, 533
BseBI CCWGG 2 cut(s) 318, 590
BseDI CCNNGG 2 cut(s) 87, 317
BseLI CCNNNNNNNGG 3 cut(s) 93, 185, 533
BseMII CTCAG 1 cut(s) 357
BseXI GCAGC 2 cut(s) 95, 148
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 3 cut(s) 93, 185, 533
BsmAI GTCTC 1 cut(s) 544
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 358
BspMI ACCTGC 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 87, 317
BssNAI GTATAC 1 cut(s) 197
BssNI GRCGYC 1 cut(s) 537
BssSI CACGAG 2 cut(s) 46, 150
Bst1107I GTATAC 1 cut(s) 197
Bst2BI CACGAG 2 cut(s) 46, 150
Bst2UI CCWGG 2 cut(s) 318, 590
Bst4CI ACNGT 2 cut(s) 91, 401
BstACI GRCGYC 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 336, 623
BstDEI CTNAG 2 cut(s) 345, 366
BstDSI CCRYGG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 334
BstMAI GTCTC 1 cut(s) 544
BstMWI GCNNNNNNNGC 4 cut(s) 42, 161, 331, 350
BstNI CCWGG 2 cut(s) 318, 590
BstSCI CCNGG 2 cut(s) 316, 588
BstV1I GCAGC 2 cut(s) 95, 148
BstZ17I GTATAC 1 cut(s) 197
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 2 cut(s) 27, 51
BtsIMutI CAGTG 1 cut(s) 397
BveI ACCTGC 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 336, 623
CfoI GCGC 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 91
CpoI CGGWCCG 1 cut(s) 91
CseI GACGC 2 cut(s) 526, 526
Csp6I GTAC 1 cut(s) 239
CspI CGGWCCG 1 cut(s) 91
CviAII CATG 3 cut(s) 245, 463, 528
CviJI RGCY 5 cut(s) 45, 86, 289, 353, 383
CviKI_1 RGCY 5 cut(s) 45, 86, 289, 353, 383
CviQI GTAC 1 cut(s) 239
DdeI CTNAG 2 cut(s) 345, 366
Ecl136II GAGCTC 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 45
EcoICRI GAGCTC 1 cut(s) 45
EcoRII CCWGG 2 cut(s) 316, 588
EcoT22I ATGCAT 1 cut(s) 464
EcoT38I GRGCYC 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 544
FaeI CATG 3 cut(s) 248, 466, 531
FaiI YATR 7 cut(s) 104, 197, 246, 460, 464, 522, 529
FaqI GGGAC 1 cut(s) 492
FatI CATG 3 cut(s) 244, 462, 527
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 2 cut(s) 84, 162
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 84, 162
FspBI CTAG 1 cut(s) 185
FspI TGCGCA 1 cut(s) 333
GlaI GCGC 1 cut(s) 333
GluI GCNGC 2 cut(s) 84, 162
GsuI CTGGAG 1 cut(s) 611
HapII CCGG 1 cut(s) 94
HgaI GACGC 2 cut(s) 526, 526
HhaI GCGC 1 cut(s) 334
Hin1I GRCGYC 1 cut(s) 537
Hin1II CATG 3 cut(s) 248, 466, 531
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HinfI GANTC 2 cut(s) 271, 593
HpaII CCGG 1 cut(s) 94
HphI GGTGA 4 cut(s) 66, 121, 402, 617
Hpy166II GTNNAC 1 cut(s) 197
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 3 cut(s) 129, 150, 579
Hpy8I GTNNAC 1 cut(s) 197
HpyAV CCTTC 3 cut(s) 343, 404, 469
HpyCH4III ACNGT 2 cut(s) 91, 401
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 4 cut(s) 164, 209, 462, 625
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 161, 331, 350
HpyF3I CTNAG 2 cut(s) 345, 366
HpySE526I ACGT 1 cut(s) 31
Hsp92I GRCGYC 1 cut(s) 537
Hsp92II CATG 3 cut(s) 248, 466, 531
HspAI GCGC 1 cut(s) 332
Lsp1109I GCAGC 2 cut(s) 95, 148
LweI GCATC 1 cut(s) 64
MaeI CTAG 1 cut(s) 185
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 4 cut(s) 109, 147, 370, 514
MboII GAAGA 2 cut(s) 148, 151
MfeI CAATTG 1 cut(s) 482
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 5 cut(s) 180, 375, 426, 482, 549
MlyI GAGTC 2 cut(s) 265, 602
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 4 cut(s) 19, 272, 361, 603
Mph1103I ATGCAT 1 cut(s) 464
MseI TTAA 3 cut(s) 230, 434, 548
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 2 cut(s) 318, 590
MunI CAATTG 1 cut(s) 482
MvaI CCWGG 2 cut(s) 318, 590
MwoI GCNNNNNNNGC 4 cut(s) 42, 161, 331, 350
NlaIII CATG 3 cut(s) 248, 466, 531
NmuCI GTSAC 3 cut(s) 109, 147, 514
NsbI TGCGCA 1 cut(s) 333
NsiI ATGCAT 1 cut(s) 464
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 2 cut(s) 85, 163
PleI GAGTC 2 cut(s) 265, 601
PpsI GAGTC 2 cut(s) 265, 601
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 2 cut(s) 316, 588
PspGI CCWGG 2 cut(s) 316, 588
PspPI GGNCC 1 cut(s) 91
RsaI GTAC 1 cut(s) 240
RsaNI GTAC 1 cut(s) 239
Rsr2I CGGWCCG 1 cut(s) 91
RsrII CGGWCCG 1 cut(s) 91
SacI GAGCTC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 230, 434, 548
SatI GCNGC 2 cut(s) 84, 162
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 2 cut(s) 265, 602
ScrFI CCNGG 2 cut(s) 318, 590
SduI GDGCHC 1 cut(s) 47
SetI ASST 7 cut(s) 34, 47, 71, 190, 291, 372, 614
SfaNI GCATC 1 cut(s) 64
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
Sse9I AATT 5 cut(s) 180, 375, 426, 482, 549
SspMI CTAG 1 cut(s) 185
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 2 cut(s) 316, 588
TaaI ACNGT 2 cut(s) 91, 401
TaiI ACGT 1 cut(s) 34
TasI AATT 5 cut(s) 180, 375, 426, 482, 549
TatI WGTACW 1 cut(s) 238
Tru1I TTAA 3 cut(s) 230, 434, 548
Tru9I TTAA 3 cut(s) 230, 434, 548
TscAI CASTG 1 cut(s) 404
TseFI GTSAC 3 cut(s) 109, 147, 514
TseI GCWGC 2 cut(s) 83, 161
Tsp45I GTSAC 3 cut(s) 109, 147, 514
TspDTI ATGAA 2 cut(s) 488, 575
TspRI CASTG 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 196
XspI CTAG 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.