Rmu_sc0002105.1_g000028

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002105.1
OG0017014
Physical Location & Seq
Reverse (-)
142265 .. 142933
669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
ctgagaggttgccatcgtggaagagtaggagcactagcatggaatagtcacattcttacaactgggggaagggatggttgtattgtaaacaatgatgtaagggtgaaatctcacatcgtggaaaactacaggggtcaccggcaggaggtgtgtggattgaagtggtcacactcagagcagaatttagctagtggagggaatgatagtcgggtcttcatatgggacaggtctgcggcaacctctgcaaggcagtcacagtggcttcataagcttgatggccacagtggagcagtcaaggcccttgcttggtgcccatttcaagggaatttgctagcctctggtggaggtgaggatgaccggtgcattaagttttggaatgcatgcacaggctcgtgcttgaattcaatcgacacaggctcacatgtgactgctcttctgtggaatcaccatgaccgggaactgttgagttctcacggttttaccaaaaaccagctaatattgtgggactacccctcaatggtgaggaggacagaaatcactgggcatacatcgaggattctattcatgactcagagcccggatggatgcactgtggcttctgcagctggtggggatgatgagtctgtgatgttatggaatgtgtttatggaagttcctaagcctgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

222

Amino Acids

24.5

Weight (kDa)

7.79

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 4 - 212 4.8e-79 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 5 - 214 3.2e-27 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 15 - 156 1.5e-18 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 15 - 157 5.6e-16 WDR5 beta-propeller domain
WD40_Prp19 PF24814 15 - 213 6e-21 Prp19 WD40 domain
WD40_Gbeta PF25391 34 - 214 1.1e-10 G protein beta WD-40 repeat protein
WD40 PF00400 41 - 75 6.5e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 41 - 130 1.1e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR19_1st PF23389 53 - 213 8.4e-07 WDR19 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 61 - 140 7.2e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 88 - 126 7.1e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 309
AciI CCGC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 3 cut(s) 181, 325, 400
AdeI CACNNNGTG 1 cut(s) 118
AfiI CCNNNNNNNGG 6 cut(s) 145, 246, 306, 320, 454, 517
AflIII ACRYGT 1 cut(s) 421
AgeI ACCGGT 1 cut(s) 357
AgsI TTSAA 4 cut(s) 160, 320, 400, 405
AluBI AGCT 4 cut(s) 188, 271, 493, 605
AluI AGCT 4 cut(s) 188, 271, 493, 605
Alw21I GWGCWC 1 cut(s) 34
AoxI GGCC 2 cut(s) 277, 297
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 3 cut(s) 181, 325, 400
AsiGI ACCGGT 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 2 cut(s) 455, 578
AsuHPI GGTGA 5 cut(s) 115, 128, 359, 437, 532
AsuNHI GCTAGC 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 314
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 578
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 614
BccI CCATC 4 cut(s) 21, 68, 269, 575
BcnI CCSGG 2 cut(s) 455, 578
BfaI CTAG 3 cut(s) 35, 189, 332
BfmI CTRYAG 2 cut(s) 127, 600
BisI GCNGC 2 cut(s) 234, 603
BlsI GCNGC 2 cut(s) 235, 604
Bme1390I CCNGG 2 cut(s) 455, 578
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 311
BmrFI CCNGG 2 cut(s) 455, 578
BmrI ACTGGG 2 cut(s) 72, 549
BmsI GCATC 1 cut(s) 575
BmtI GCTAGC 1 cut(s) 335
BmuI ACTGGG 2 cut(s) 72, 549
BpiI GAAGAC 1 cut(s) 205
Bpu10I CCTNAGC 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 455, 578
BsaWI WCCGGW 1 cut(s) 357
Bsc4I CCNNNNNNNGG 6 cut(s) 145, 246, 306, 320, 454, 517
Bse118I RCCGGY 2 cut(s) 138, 357
Bse1I ACTGG 2 cut(s) 67, 544
BseGI GGATG 5 cut(s) 79, 358, 586, 590, 619
BseLI CCNNNNNNNGG 6 cut(s) 145, 246, 306, 320, 454, 517
BseMII CTCAG 2 cut(s) 186, 584
BseNI ACTGG 2 cut(s) 67, 544
BseRI GAGGAG 1 cut(s) 538
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 1 cut(s) 614
BshFI GGCC 2 cut(s) 279, 299
BshNI GGYRCC 1 cut(s) 309
BshTI ACCGGT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 34
BsiSI CCGG 4 cut(s) 139, 358, 454, 578
BslFI GGGAC 2 cut(s) 236, 518
BslI CCNNNNNNNGG 6 cut(s) 145, 246, 306, 320, 454, 517
BsmFI GGGAC 2 cut(s) 236, 518
BsmI GAATGC 1 cut(s) 382
BsnI GGCC 2 cut(s) 279, 299
Bsp1286I GDGCHC 3 cut(s) 34, 314, 578
BspACI CCGC 1 cut(s) 233
BspANI GGCC 2 cut(s) 279, 299
BspCNI CTCAG 2 cut(s) 185, 583
BspHI TCATGA 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 335
BspQI GCTCTTC 1 cut(s) 438
BspT107I GGYRCC 1 cut(s) 309
BsrFI RCCGGY 2 cut(s) 138, 357
BsrI ACTGG 2 cut(s) 67, 544
BssAI RCCGGY 2 cut(s) 138, 357
BssSI CACGAG 1 cut(s) 391
Bst2BI CACGAG 1 cut(s) 391
Bst4CI ACNGT 5 cut(s) 258, 284, 462, 476, 592
Bst6I CTCTTC 2 cut(s) 16, 438
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 2 cut(s) 333, 382
BstDEI CTNAG 4 cut(s) 2, 172, 570, 657
BstEII GGTNACC 1 cut(s) 134
BstENI CCTNNNNNAGG 1 cut(s) 244
BstF5I GGATG 5 cut(s) 79, 358, 586, 590, 619
BstMWI GCNNNNNNNGC 4 cut(s) 242, 268, 296, 602
BstNSI RCATGY 2 cut(s) 384, 425
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 453, 576
BstSFI CTRYAG 2 cut(s) 127, 600
BstSLI GKGCMC 1 cut(s) 314
BstV1I GCAGC 1 cut(s) 614
BstV2I GAAGAC 1 cut(s) 205
BsuRI GGCC 2 cut(s) 279, 299
BtsCI GGATG 5 cut(s) 79, 358, 586, 590, 619
BtsIMutI CAGTG 4 cut(s) 263, 289, 537, 588
Cac8I GCNNGC 2 cut(s) 333, 382
CciI TCATGA 1 cut(s) 564
Cfr10I RCCGGY 2 cut(s) 138, 357
Cfr13I GGNCC 1 cut(s) 298
CspAI ACCGGT 1 cut(s) 357
CviAII CATG 5 cut(s) 39, 381, 422, 449, 565
DdeI CTNAG 4 cut(s) 2, 172, 570, 657
DraIII CACNNNGTG 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 16, 438
EarI CTCTTC 2 cut(s) 16, 438
Eco24I GRGCYC 1 cut(s) 578
Eco91I GGTNACC 1 cut(s) 134
EcoNI CCTNNNNNAGG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 298
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 400
EcoT22I ATGCAT 1 cut(s) 382
EcoT38I GRGCYC 1 cut(s) 578
FaeI CATG 5 cut(s) 42, 384, 425, 452, 568
FaqI GGGAC 2 cut(s) 236, 518
FatI CATG 5 cut(s) 38, 380, 421, 448, 564
FauNDI CATATG 1 cut(s) 218
Fnu4HI GCNGC 2 cut(s) 234, 603
FokI GGATG 5 cut(s) 86, 365, 593, 597, 626
FriOI GRGCYC 1 cut(s) 578
Fsp4HI GCNGC 2 cut(s) 234, 603
FspBI CTAG 3 cut(s) 35, 189, 332
GluI GCNGC 2 cut(s) 234, 603
HaeIII GGCC 2 cut(s) 279, 299
HapII CCGG 4 cut(s) 139, 358, 454, 578
Hin1II CATG 5 cut(s) 42, 384, 425, 452, 568
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 4 cut(s) 442, 556, 568, 620
HpaII CCGG 4 cut(s) 139, 358, 454, 578
HphI GGTGA 5 cut(s) 115, 128, 359, 437, 532
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 2 cut(s) 175, 573
Hpy188III TCNNGA 1 cut(s) 565
Hpy8I GTNNAC 1 cut(s) 88
HpyAV CCTTC 1 cut(s) 63
HpyCH4III ACNGT 5 cut(s) 258, 284, 462, 476, 592
HpyCH4V TGCA 6 cut(s) 245, 363, 380, 384, 588, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 268, 296, 602
HpyF3I CTNAG 4 cut(s) 2, 172, 570, 657
Hsp92II CATG 5 cut(s) 42, 384, 425, 452, 568
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 2 cut(s) 29, 287
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 1 cut(s) 575
MaeI CTAG 3 cut(s) 35, 189, 332
MaeIII GTNAC 5 cut(s) 47, 134, 165, 252, 424
MboII GAAGA 3 cut(s) 33, 205, 425
MhlI GDGCHC 3 cut(s) 34, 314, 578
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 3 cut(s) 181, 325, 400
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 2 cut(s) 562, 629
Mox20I TGGCCA 1 cut(s) 279
Mph1103I ATGCAT 1 cut(s) 382
MscI TGGCCA 1 cut(s) 279
MseI TTAA 1 cut(s) 366
MslI CAYNNNNRTG 1 cut(s) 37
Msp20I TGGCCA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 4 cut(s) 139, 358, 454, 578
MspR9I CCNGG 2 cut(s) 455, 578
Mva1269I GAATGC 1 cut(s) 382
MwoI GCNNNNNNNGC 4 cut(s) 242, 268, 296, 602
NciI CCSGG 2 cut(s) 455, 578
NdeI CATATG 1 cut(s) 218
NheI GCTAGC 1 cut(s) 331
NlaIII CATG 5 cut(s) 42, 384, 425, 452, 568
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 5 cut(s) 47, 134, 165, 252, 424
NsiI ATGCAT 1 cut(s) 382
NspI RCATGY 2 cut(s) 384, 425
PaeI GCATGC 1 cut(s) 384
PagI TCATGA 1 cut(s) 564
PciI ACATGT 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 438
PctI GAATGC 1 cut(s) 382
PfeI GAWTC 2 cut(s) 442, 556
PinAI ACCGGT 1 cut(s) 357
PkrI GCNGC 2 cut(s) 235, 604
PleI GAGTC 2 cut(s) 562, 628
PpsI GAGTC 2 cut(s) 562, 628
PscI ACATGT 1 cut(s) 421
PspEI GGTNACC 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 605
RseI CAYNNNNRTG 1 cut(s) 37
SapI GCTCTTC 1 cut(s) 438
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 2 cut(s) 234, 603
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 2 cut(s) 562, 629
ScrFI CCNGG 2 cut(s) 455, 578
SduI GDGCHC 3 cut(s) 34, 314, 578
SetI ASST 9 cut(s) 10, 150, 190, 230, 242, 273, 349, 495, 607
SfaNI GCATC 1 cut(s) 575
SfcI CTRYAG 2 cut(s) 127, 600
SmiMI CAYNNNNRTG 1 cut(s) 37
SphI GCATGC 1 cut(s) 384
Sse9I AATT 3 cut(s) 181, 325, 400
SsiI CCGC 1 cut(s) 233
SspI AATATT 1 cut(s) 498
SspMI CTAG 3 cut(s) 35, 189, 332
StyD4I CCNGG 2 cut(s) 453, 576
TaaI ACNGT 5 cut(s) 258, 284, 462, 476, 592
TaqI TCGA 2 cut(s) 408, 551
TasI AATT 3 cut(s) 181, 325, 400
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 2 cut(s) 442, 556
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 4 cut(s) 263, 289, 544, 595
TseFI GTSAC 5 cut(s) 47, 134, 165, 252, 424
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 5 cut(s) 47, 134, 165, 252, 424
TspDTI ATGAA 3 cut(s) 205, 254, 553
TspRI CASTG 4 cut(s) 263, 289, 544, 595
XagI CCTNNNNNAGG 1 cut(s) 244
XapI RAATTY 3 cut(s) 181, 325, 400
XceI RCATGY 2 cut(s) 384, 425
XspI CTAG 3 cut(s) 35, 189, 332
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.