Rh7AG338500

Cell division cycle 20.2, cofactor of APC complex-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0017014
Physical Location & Seq
Forward (+)
42633355 .. 42636434
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG338500.1

Sequence Viewer

Length: 813 bp
ATGTCAGGTGATTCCCCTATAAAGATTATGACTTGGAGCCGTATCCAAGGTAAGTATATTTGGAGAAATAGGAAGAATAGGGAAAATTTAGATTTGGATAGGTTCATACCCAATTACGAGGCATTACACTTAGATTATGCTAGCTATATAGTGGCAGGAGGGAGTACCACCAAGGAGATTCTAGAAAGAACTGAGAGGGTGAAATCTCACATCGTGGAAAACTACAGGGGTCACCGGCAGGAGGTGTGTGGATTGAAGTGGTCACACTCAGGGCAGAATTTAGCTAGTGGAGGGAATGATAGTCGGGTCTTCATATGGGACAGGTCTGCGGCAACCTCTGCAAGGCAGTCACAGTGGCTTCACAAGCTTGATGGCCACAGTGGAGCAGTCAAGGCCCTTGCTTGGTGCCCATTTCAAGGGAATTTGCTAGCCTCTGGTGGAGGTGAGGATGACCGGTGCATCAAGTTTTGGAATGCACGCACAGGCTCGTGCTTGAATTCAATCGACACGGGCTCACAGGTGACTGCTCTTCTGTGGAATCACCATGACCGGGAACTGTTGAGTTCTCACGGTTTTACCAAAAACCAGCTAATATTGTGGGACTACCCCTCAATGGTGAGGAGGATAGAAATCACTGGGCATACATCGAGGATTCTATTCATGACTCAGAGCCCGGATGGATGCACTGTGGCTTCTGCAGCTGGTGGGGATGATGAGTCTGTGATGTTATGGAATGTGTTTATGGAAGTCCCTAAGCATGTGCGAAAAGAAGATCATCTGCCATTTGCTCACTCATTCTACCACGTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

270

Amino Acids

30.68

Weight (kDa)

8.58

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 20 - 188 1.9e-18 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 44 - 244 1.4e-71 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 44 - 245 1.8e-18 Prp19 WD40 domain
WD40_Gbeta PF25391 61 - 247 3.7e-11 G protein beta WD-40 repeat protein
WDR55 PF24796 66 - 191 8.4e-07 WDR55
Beta-prop_WDR5 PF25175 68 - 189 2.4e-16 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 70 - 167 1.7e-07 TEP-1 second beta-propeller
WD40 PF00400 73 - 107 1.5e-07 WD domain, G-beta repeat
Beta-prop_EML PF23409 75 - 201 7.1e-06 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 76 - 189 2.1e-06 EIF3I
Beta-prop_THOC3 PF25174 78 - 186 2.4e-21 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 79 - 189 3.1e-06 Aladin seven-bladed propeller
Beta-prop_WDR19_1st PF23389 85 - 246 2.1e-08 WDR19 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 94 - 174 6.8e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 120 - 158 9.7e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 141 - 260 2.7e-06 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 173 - 246 2e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 405
AciI CCGC 1 cut(s) 329
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 4 cut(s) 85, 277, 421, 496
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 1 cut(s) 166
AfiI CCNNNNNNNGG 6 cut(s) 241, 342, 402, 416, 550, 613
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 4 cut(s) 256, 416, 496, 501
AluBI AGCT 5 cut(s) 144, 284, 367, 589, 701
AluI AGCT 5 cut(s) 144, 284, 367, 589, 701
AoxI GGCC 2 cut(s) 373, 393
ApeKI GCWGC 1 cut(s) 698
ApoI RAATTY 4 cut(s) 85, 277, 421, 496
AsiGI ACCGGT 1 cut(s) 453
AspS9I GGNCC 1 cut(s) 394
AsuC2I CCSGG 2 cut(s) 551, 674
AsuHPI GGTGA 7 cut(s) 20, 211, 224, 455, 532, 533, 628
AsuNHI GCTAGC 2 cut(s) 140, 427
BaeGI GKGCMC 1 cut(s) 410
BalI TGGCCA 1 cut(s) 375
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 2 cut(s) 515, 674
BauI CACGAG 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 301
BbvI GCAGC 1 cut(s) 710
BccI CCATC 2 cut(s) 365, 671
BceAI ACGGC 1 cut(s) 24
BciVI GTATCC 1 cut(s) 53
BcnI CCSGG 2 cut(s) 551, 674
BfaI CTAG 4 cut(s) 141, 182, 285, 428
BfmI CTRYAG 2 cut(s) 223, 696
BfuI GTATCC 1 cut(s) 53
BisI GCNGC 2 cut(s) 330, 699
BlsI GCNGC 2 cut(s) 331, 700
Bme1390I CCNGG 2 cut(s) 551, 674
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 2 cut(s) 38, 407
BmrFI CCNGG 2 cut(s) 551, 674
BmrI ACTGGG 1 cut(s) 645
BmsI GCATC 2 cut(s) 468, 671
BmtI GCTAGC 2 cut(s) 144, 431
BmuI ACTGGG 1 cut(s) 645
BpiI GAAGAC 1 cut(s) 301
Bpu10I CCTNAGC 1 cut(s) 753
BpuMI CCSGG 2 cut(s) 551, 674
BsaBI GATNNNNATC 1 cut(s) 629
BsaJI CCNNGG 2 cut(s) 46, 171
BsaWI WCCGGW 1 cut(s) 453
Bsc4I CCNNNNNNNGG 6 cut(s) 241, 342, 402, 416, 550, 613
Bse118I RCCGGY 2 cut(s) 234, 453
Bse1I ACTGG 1 cut(s) 640
Bse8I GATNNNNATC 1 cut(s) 629
BseDI CCNNGG 2 cut(s) 46, 171
BseGI GGATG 4 cut(s) 454, 682, 686, 715
BseJI GATNNNNATC 1 cut(s) 629
BseLI CCNNNNNNNGG 6 cut(s) 241, 342, 402, 416, 550, 613
BseMII CTCAG 3 cut(s) 183, 282, 680
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 1 cut(s) 634
BseSI GKGCMC 1 cut(s) 410
BseXI GCAGC 1 cut(s) 710
BshFI GGCC 2 cut(s) 375, 395
BshNI GGYRCC 1 cut(s) 405
BshTI ACCGGT 1 cut(s) 453
BsiSI CCGG 4 cut(s) 235, 454, 550, 674
BslFI GGGAC 3 cut(s) 332, 614, 734
BslI CCNNNNNNNGG 6 cut(s) 241, 342, 402, 416, 550, 613
BsmFI GGGAC 3 cut(s) 332, 614, 734
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 2 cut(s) 375, 395
Bsp1286I GDGCHC 3 cut(s) 410, 515, 674
Bsp143I GATC 1 cut(s) 772
BspACI CCGC 1 cut(s) 329
BspANI GGCC 2 cut(s) 375, 395
BspCNI CTCAG 3 cut(s) 184, 281, 679
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 2 cut(s) 38, 407
BspMAI CTGCAG 1 cut(s) 700
BspOI GCTAGC 2 cut(s) 144, 431
BspQI GCTCTTC 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 405
BsrFI RCCGGY 2 cut(s) 234, 453
BsrI ACTGG 1 cut(s) 640
BssAI RCCGGY 2 cut(s) 234, 453
BssECI CCNNGG 2 cut(s) 46, 171
BssMI GATC 1 cut(s) 772
BssSI CACGAG 1 cut(s) 487
BssT1I CCWWGG 2 cut(s) 46, 171
Bst2BI CACGAG 1 cut(s) 487
Bst4CI ACNGT 5 cut(s) 354, 380, 558, 572, 688
Bst6I CTCTTC 1 cut(s) 534
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 142, 429, 478
BstDEI CTNAG 5 cut(s) 130, 192, 268, 666, 753
BstEII GGTNACC 1 cut(s) 230
BstENI CCTNNNNNAGG 1 cut(s) 340
BstF5I GGATG 4 cut(s) 454, 682, 686, 715
BstKTI GATC 1 cut(s) 775
BstMBI GATC 1 cut(s) 772
BstMWI GCNNNNNNNGC 4 cut(s) 338, 364, 392, 698
BstNSI RCATGY 1 cut(s) 761
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 2 cut(s) 549, 672
BstSFI CTRYAG 2 cut(s) 223, 696
BstSLI GKGCMC 1 cut(s) 410
BstV1I GCAGC 1 cut(s) 710
BstV2I GAAGAC 1 cut(s) 301
BsuI GTATCC 1 cut(s) 53
BsuRI GGCC 2 cut(s) 375, 395
BtsCI GGATG 4 cut(s) 454, 682, 686, 715
BtsIMutI CAGTG 4 cut(s) 359, 385, 633, 684
Cac8I GCNNGC 3 cut(s) 142, 429, 478
CciI TCATGA 1 cut(s) 660
Cfr10I RCCGGY 2 cut(s) 234, 453
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 1 cut(s) 165
CspAI ACCGGT 1 cut(s) 453
CspCI CAANNNNNGTGG 1 cut(s) 791
CviAII CATG 3 cut(s) 545, 661, 758
CviQI GTAC 1 cut(s) 165
DdeI CTNAG 5 cut(s) 130, 192, 268, 666, 753
DpnI GATC 1 cut(s) 774
DpnII GATC 1 cut(s) 772
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 534
EarI CTCTTC 1 cut(s) 534
Eco130I CCWWGG 2 cut(s) 46, 171
Eco24I GRGCYC 2 cut(s) 515, 674
Eco91I GGTNACC 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 230
EcoRI GAATTC 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 46, 171
EcoT38I GRGCYC 2 cut(s) 515, 674
ErhI CCWWGG 2 cut(s) 46, 171
FaeI CATG 3 cut(s) 548, 664, 761
FaqI GGGAC 3 cut(s) 332, 614, 734
FatI CATG 3 cut(s) 544, 660, 757
FauNDI CATATG 1 cut(s) 314
Fnu4HI GCNGC 2 cut(s) 330, 699
FokI GGATG 4 cut(s) 461, 689, 693, 722
FriOI GRGCYC 2 cut(s) 515, 674
Fsp4HI GCNGC 2 cut(s) 330, 699
FspBI CTAG 4 cut(s) 141, 182, 285, 428
GluI GCNGC 2 cut(s) 330, 699
HaeIII GGCC 2 cut(s) 375, 395
HapII CCGG 4 cut(s) 235, 454, 550, 674
Hin1II CATG 3 cut(s) 548, 664, 761
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 6 cut(s) 11, 178, 538, 652, 664, 716
HpaII CCGG 4 cut(s) 235, 454, 550, 674
HphI GGTGA 7 cut(s) 20, 211, 224, 455, 532, 533, 628
Hpy188I TCNGA 1 cut(s) 669
Hpy188III TCNNGA 2 cut(s) 182, 661
HpyCH4III ACNGT 5 cut(s) 354, 380, 558, 572, 688
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 5 cut(s) 341, 459, 476, 684, 698
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 364, 392, 698
HpyF3I CTNAG 5 cut(s) 130, 192, 268, 666, 753
HpySE526I ACGT 1 cut(s) 804
Hsp92II CATG 3 cut(s) 548, 664, 761
Kzo9I GATC 1 cut(s) 772
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 2 cut(s) 36, 383
Lsp1109I GCAGC 1 cut(s) 710
LweI GCATC 2 cut(s) 468, 671
MaeI CTAG 4 cut(s) 141, 182, 285, 428
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 4 cut(s) 230, 261, 348, 520
MalI GATC 1 cut(s) 774
MboI GATC 1 cut(s) 772
MboII GAAGA 4 cut(s) 85, 301, 521, 782
MhlI GDGCHC 3 cut(s) 410, 515, 674
MlsI TGGCCA 1 cut(s) 375
MluCI AATT 5 cut(s) 85, 112, 277, 421, 496
MluNI TGGCCA 1 cut(s) 375
MlyI GAGTC 2 cut(s) 658, 725
Mox20I TGGCCA 1 cut(s) 375
MscI TGGCCA 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 4 cut(s) 235, 454, 550, 674
MspR9I CCNGG 2 cut(s) 551, 674
Mva1269I GAATGC 1 cut(s) 478
MwoI GCNNNNNNNGC 4 cut(s) 338, 364, 392, 698
NciI CCSGG 2 cut(s) 551, 674
NdeI CATATG 1 cut(s) 314
NdeII GATC 1 cut(s) 772
NheI GCTAGC 2 cut(s) 140, 427
NlaIII CATG 3 cut(s) 548, 664, 761
NlaIV GGNNCC 2 cut(s) 38, 407
NmuCI GTSAC 4 cut(s) 230, 261, 348, 520
NspI RCATGY 1 cut(s) 761
PagI TCATGA 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 4 cut(s) 11, 178, 538, 652
PinAI ACCGGT 1 cut(s) 453
PkrI GCNGC 2 cut(s) 331, 700
PleI GAGTC 2 cut(s) 658, 724
PpsI GAGTC 2 cut(s) 658, 724
PspEI GGTNACC 1 cut(s) 230
PspN4I GGNNCC 2 cut(s) 38, 407
PspPI GGNCC 1 cut(s) 394
PstI CTGCAG 1 cut(s) 700
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 1 cut(s) 166
RsaNI GTAC 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 534
SatI GCNGC 2 cut(s) 330, 699
Sau3AI GATC 1 cut(s) 772
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 2 cut(s) 658, 725
ScrFI CCNGG 2 cut(s) 551, 674
SduI GDGCHC 3 cut(s) 410, 515, 674
SfaNI GCATC 2 cut(s) 468, 671
SfcI CTRYAG 2 cut(s) 223, 696
Sse9I AATT 5 cut(s) 85, 112, 277, 421, 496
SsiI CCGC 1 cut(s) 329
SspI AATATT 1 cut(s) 594
SspMI CTAG 4 cut(s) 141, 182, 285, 428
StyD4I CCNGG 2 cut(s) 549, 672
StyI CCWWGG 2 cut(s) 46, 171
TaaI ACNGT 5 cut(s) 354, 380, 558, 572, 688
TaiI ACGT 1 cut(s) 807
TaqI TCGA 2 cut(s) 504, 647
TasI AATT 5 cut(s) 85, 112, 277, 421, 496
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 4 cut(s) 11, 178, 538, 652
TscAI CASTG 4 cut(s) 359, 385, 640, 691
TseFI GTSAC 4 cut(s) 230, 261, 348, 520
TseI GCWGC 1 cut(s) 698
Tsp45I GTSAC 4 cut(s) 230, 261, 348, 520
TspDTI ATGAA 3 cut(s) 94, 301, 649
TspRI CASTG 4 cut(s) 359, 385, 640, 691
XagI CCTNNNNNAGG 1 cut(s) 340
XapI RAATTY 4 cut(s) 85, 277, 421, 496
XbaI TCTAGA 1 cut(s) 181
XceI RCATGY 1 cut(s) 761
XspI CTAG 4 cut(s) 141, 182, 285, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.