Rmu_sc0002111.1_g000005
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002111.1
OG0016035
Physical Location & Seq
Forward (+)
11463 .. 14557
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2988 bp
atggtttctctgcaacgctgcatgggcgacgaccccatcccggcacagctaaactacgacgttttgggcctcctcgtcttcaaatccgacctccacgacccttcttcctacctcggttcctggaacgaagacgacgactccccatgttcatggaacttcatccaatgcaaccctaccaccggaagagtctctcacctctccctcgacggcttaggacttcagggcaaaatcggaaaaggtcttcagaatcttcagcacctcaaggtactctctttatccaacaacaacttcactggtgagcttagtccagaaaaacttgctcttcctccaaatctccaaacactcaacttaagccgaaacagtttctcgggactagttcccactactcttgtgaacttcagctccatcagatttcttgatctttctgagaactcactctctggttcactccctgattcgctctttgatgcttgtttttcgcttcgctacctttctttatctggaaacttgctagaagggaacttgcctagtactctttccagatgctcttctttgaatggcctcaacatctccaacaaccgtttctccggtaacccggatttcgaatccgggatttggtccttggaacggcttcgttctttggatctttcaaacaatgctctttccgggtttgtgccaaaagggatatcagccacacataatttgaaagagcttttaatccagaggaaccacttttctgggccaatgccttttgatattggactgtgtccgcacttgggtaggattgatttcagtgaaaatctgttcactggtgagttaccgcagtcacttcagaagttgaattccttgacatttatgagtctttcagataacatgttcaatggtgattttcctcaatggattgggaacatgagcagcctacagtacttggacttttccaacaatggcttcacgggaagcctcccaccatcaatgggtgaccttacatctctcagttatctaagtctgtctaacaacaaacttgttgggacacttccaacttctcttgtctactgcaacaagttgtctgtgattcggttgaggggtaatgatttcagtgggagcataccagaaggtttgtttggtctgggcttggaagagatagatttttcggatatgggacttacaggttcaattccacccggttcgagtaaattgtttgagtctttgaagatgttggatctatcaaggaacaatctcaaaggcaacattccagcagaggtgggacttctatccaacttgagatacttgaatctttcatggaacaatcttcaatcaaaaattcccccagagcttggattcttttcaaacctaaccgtgctagatctccgcaatagcgcattgtatggttcaatcccaggagatatatgtgattctgggagtttggatattctccaattggatggtaattcattgactggcccaattcctgatgagattgggaattgctcatccctctatttgatgagtttgtcacataacaatttaagtggtgctattccaaaatccatttcgaagttgagcaagctcgagattctcaagttagagttcaatcagttgggtggagagataccgcaagagcttggaaaattggaaaatcttcttgctgtgaacatttcatacaacagattaacagggaggcttcctgtagggagtgtgtttcagagtttggatcaaagtgctttgcaaggaaacttgggtatttgttcaccattgttgaagggaccatgtacaatgaatgtacctaagcctcttgttcttgatccaaatgcctaccccaaccaaatgggtggtggggatcatagattccaaggcgaatcttcagggtcccgaaaaggacatcaccatatgttccttagcatatctgctattgtggcaatttcagctgctacattgatcgctgttggagtaattgtcattacccttctaaatgcatctgcaagaaggaggcctgcatttgtggaaactgccttggagagcatgtgttcgagctcttcgcgatcgagtagtttggcatcagggaagctcattctgtttgactcaaggtcatcgccggattggatcagcagtcctgaatcactactaaacaaggcctctgagctcggggagggagtctttgggacagtctacaaagttccactgggagctcagggaagaatggtggcaatcaaaaagcttgtgacaacaaacatactccaatgtcttgaagattttgacagagaagttcgagtccttggaaaggcgaggcacccgaatctagtagccctgaaaggatattactggactccacagatgcagctacttgtaaatgagtatgcccccaatggcaacctgcaatccaaactacatgataggcttcattcaagttcacctctttcatgggatgataggttcaaaatcctgcttggaatagcaaggggactttcccatttgcaccattctttccgcccgcccatcattcactacaacataaaaccaagtaacatattgctcgatgaaaacctcaacccgaagatttctgattttgcacttgcaaggctactaaccaagatcgacaggcacgtagtgagtaacagatttcagagtgcattgggctatgtggcaccggaactagcttgccagagcctaagggtaaacgagaaatgcgatgtgtatggtttcggtgtcttgatccttgagcttgtcactggtagaaggccagtggaatatggggaagacaatgtggtgatattgactgatcatgtgagggttttgcttgaacaaggcaatgtgttgggaagcattgatcatagcatgggggagtacccagaggatgaagtcctaccagtgttcaagttggccttggtgtgcacttctcagataccatcttgcaggccaaccatggcagaagtggtgcaaattcttcagattatcaagactccacttccacagagaattgagcgtttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

995

Amino Acids

108.73

Weight (kDa)

6.05

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 17 - 57 5.2e-11 Leucine rich repeat N-terminal domain
LRR_8 PF13855 86 - 146 2.7e-08 Leucine rich repeat
LRR_14 PF23598 207 - 340 1.2e-06 Leucine-rich repeat region
LRR_14 PF23598 275 - 358 5.7e-06 Leucine-rich repeat region
LRR_8 PF13855 298 - 341 3.3e-06 Leucine rich repeat
LRR_4 PF12799 306 - 345 5.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 390 - 437 2.6e-06 Leucine rich repeat
LRR_14 PF23598 399 - 555 2.3e-14 Leucine-rich repeat region
Pkinase PF00069 710 - 979 1.1e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 710 - 980 3e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28040
fragaria_vesca FvH4_4g12350
malus_domestica MD04G1027600.v1.1
prunus_persica Prupe.1G177000_v2.0.a1
pyrus_communis pycom04g02420
rosa_chinensis RchiOBHm_Chr4g0410441
rosa_laevigata RLG00000008430
rosa_multiflora Rmu_sc0002111.1_g000005
rosa_roxburghii Rroxscaffold_5G00355250
rosa_rugosa Rorug04G0102700
rosa_samantha Rh4AG160700 Rh4BG159900 Rh4CG172800 Rh4DG155600
rosa_wichuraiana Rw4G013390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2379
AccB1I GGYRCC 2 cut(s) 2286, 2641
AccB7I CCANNNNNTGG 1 cut(s) 1334
AccI GTMKAC 2 cut(s) 1050, 2166
AccII CGCG 1 cut(s) 2038
AciI CCGC 6 cut(s) 770, 821, 1369, 1613, 2485, 2489
AclWI GGATC 7 cut(s) 651, 1227, 1719, 1796, 1845, 2108, 2704
AcsI RAATTY 3 cut(s) 841, 1320, 2937
AcuI CTGAAG 7 cut(s) 203, 227, 236, 382, 815, 1845, 2927
AdeI CACNNNGTG 1 cut(s) 2605
AfaI GTAC 6 cut(s) 267, 532, 926, 1771, 1782, 2843
AfiI CCNNNNNNNGG 8 cut(s) 40, 179, 615, 627, 776, 974, 1334, 2278
AflII CTTAAG 1 cut(s) 349
AflIII ACRYGT 1 cut(s) 873
AhdI GACNNNNNGTC 1 cut(s) 2083
AhlI ACTAGT 1 cut(s) 373
AjnI CCWGG 2 cut(s) 119, 1396
AloI GAACNNNNNNTCC 4 cut(s) 386, 418, 2414, 2446
Alw21I GWGCWC 4 cut(s) 2033, 2142, 2188, 2891
Alw26I GTCTC 1 cut(s) 193
Alw44I GTGCAC 1 cut(s) 2887
AlwI GGATC 7 cut(s) 651, 1227, 1719, 1796, 1845, 2108, 2704
Ama87I CYCGRG 3 cut(s) 367, 1568, 2141
AoxI GGCC 9 cut(s) 67, 559, 740, 1459, 1988, 2130, 2735, 2877, 2912
ApaLI GTGCAC 1 cut(s) 2887
ApeKI GCWGC 4 cut(s) 18, 915, 1925, 2335
ApoI RAATTY 3 cut(s) 841, 1320, 2937
Asp700I GAANNNNTTC 2 cut(s) 630, 1638
AspLEI GCGC 1 cut(s) 1379
AspS9I GGNCC 6 cut(s) 67, 618, 740, 1460, 1763, 1866
AsuC2I CCSGG 5 cut(s) 41, 596, 610, 667, 1182
AsuHPI GGTGA 9 cut(s) 185, 308, 824, 896, 989, 1740, 1874, 2400, 2776
AsuII TTCGAA 2 cut(s) 603, 1553
AvaI CYCGRG 3 cut(s) 367, 1568, 2141
AvaII GGWCC 3 cut(s) 618, 1763, 1866
AxyI CCTNAGG 1 cut(s) 2666
BaeGI GKGCMC 1 cut(s) 2891
BanI GGYRCC 2 cut(s) 2286, 2641
BanII GRGCYC 3 cut(s) 2033, 2142, 2188
BbsI GAAGAC 4 cut(s) 70, 135, 233, 2760
Bbv12I GWGCWC 4 cut(s) 2033, 2142, 2188, 2891
BbvI GCAGC 4 cut(s) 5, 927, 1912, 2347
BccI CCATC 6 cut(s) 44, 413, 976, 1436, 2501, 2911
BceAI ACGGC 2 cut(s) 223, 644
BciT130I CCWGG 2 cut(s) 121, 1398
BclI TGATCA 2 cut(s) 2776, 2824
BcnI CCSGG 5 cut(s) 41, 596, 610, 667, 1182
BcoDI GTCTC 1 cut(s) 193
BcuI ACTAGT 1 cut(s) 373
BfaI CTAG 6 cut(s) 374, 512, 528, 1361, 2297, 2651
BfmI CTRYAG 2 cut(s) 920, 1686
BfrI CTTAAG 1 cut(s) 349
BfuAI ACCTGC 1 cut(s) 2379
BglII AGATCT 1 cut(s) 1363
BisI GCNGC 4 cut(s) 19, 916, 1926, 2336
BlsI GCNGC 4 cut(s) 20, 917, 1927, 2337
BmcAI AGTACT 2 cut(s) 532, 926
Bme1390I CCNGG 7 cut(s) 41, 121, 596, 610, 667, 1182, 1398
Bme18I GGWCC 3 cut(s) 618, 1763, 1866
BmeRI GACNNNNNGTC 1 cut(s) 2083
BmeT110I CYCGRG 3 cut(s) 367, 1568, 2141
BmgT120I GGNCC 6 cut(s) 67, 618, 740, 1460, 1763, 1866
BmiI GGNNCC 7 cut(s) 118, 728, 1764, 1867, 1868, 2288, 2643
BmrFI CCNGG 7 cut(s) 41, 121, 596, 610, 667, 1182, 1398
BmrI ACTGGG 1 cut(s) 2189
BmsI GCATC 5 cut(s) 457, 533, 1982, 2063, 2322
BmuI ACTGGG 1 cut(s) 2189
BpiI GAAGAC 4 cut(s) 70, 135, 233, 2760
Bpu10I CCTNAGC 4 cut(s) 211, 1785, 1895, 2187
Bpu14I TTCGAA 2 cut(s) 603, 1553
BpuEI CTTGAG 5 cut(s) 245, 1300, 1562, 2065, 2735
BpuMI CCSGG 5 cut(s) 41, 596, 610, 667, 1182
BsaAI YACGTR 1 cut(s) 2602
BsaJI CCNNGG 8 cut(s) 112, 621, 1396, 1849, 2010, 2272, 2880, 2919
BsaWI WCCGGW 3 cut(s) 179, 587, 2644
Bsc4I CCNNNNNNNGG 8 cut(s) 40, 179, 615, 627, 776, 974, 1334, 2278
Bse1I ACTGG 8 cut(s) 298, 814, 1462, 2184, 2324, 2731, 2738, 2864
Bse21I CCTNAGG 1 cut(s) 2666
Bse3DI GCAATG 1 cut(s) 2812
BseBI CCWGG 2 cut(s) 121, 1398
BseDI CCNNGG 8 cut(s) 112, 621, 1396, 1849, 2010, 2272, 2880, 2919
BseGI GGATG 6 cut(s) 36, 159, 1447, 1490, 2428, 2857
BseLI CCNNNNNNNGG 8 cut(s) 40, 179, 615, 627, 776, 974, 1334, 2278
BseMI GCAATG 1 cut(s) 2812
BseMII CTCAG 5 cut(s) 417, 1006, 2127, 2201, 2909
BseNI ACTGG 8 cut(s) 298, 814, 1462, 2184, 2324, 2731, 2738, 2864
BseRI GAGGAG 1 cut(s) 62
BseSI GKGCMC 1 cut(s) 2891
BseXI GCAGC 4 cut(s) 5, 927, 1912, 2347
Bsh1236I CGCG 1 cut(s) 2038
Bsh1285I CGRYCG 1 cut(s) 2042
BshFI GGCC 9 cut(s) 69, 561, 742, 1461, 1990, 2132, 2737, 2879, 2914
BshNI GGYRCC 2 cut(s) 2286, 2641
BsiEI CGRYCG 1 cut(s) 2042
BsiHKAI GWGCWC 4 cut(s) 2033, 2142, 2188, 2891
BsiHKCI CYCGRG 3 cut(s) 367, 1568, 2141
BsiSI CCGG 9 cut(s) 41, 180, 588, 596, 609, 666, 1182, 2093, 2645
BslFI GGGAC 8 cut(s) 384, 1042, 1173, 1278, 1776, 1852, 2173, 2472
BslI CCNNNNNNNGG 8 cut(s) 40, 179, 615, 627, 776, 974, 1334, 2278
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 8 cut(s) 384, 1042, 1173, 1278, 1776, 1852, 2173, 2472
BsnI GGCC 9 cut(s) 69, 561, 742, 1461, 1990, 2132, 2737, 2879, 2914
BsoBI CYCGRG 3 cut(s) 367, 1568, 2141
Bsp119I TTCGAA 2 cut(s) 603, 1553
Bsp1286I GDGCHC 4 cut(s) 2033, 2142, 2188, 2891
Bsp1407I TGTACA 1 cut(s) 1769
Bsp19I CCATGG 1 cut(s) 2919
Bsp68I TCGCGA 1 cut(s) 2038
BspACI CCGC 6 cut(s) 770, 821, 1369, 1613, 2485, 2489
BspANI GGCC 9 cut(s) 69, 561, 742, 1461, 1990, 2132, 2737, 2879, 2914
BspCNI CTCAG 5 cut(s) 418, 1005, 2128, 2200, 2908
BspFNI CGCG 1 cut(s) 2038
BspLI GGNNCC 7 cut(s) 118, 728, 1764, 1867, 1868, 2288, 2643
BspMI ACCTGC 1 cut(s) 2379
BspPI GGATC 7 cut(s) 651, 1227, 1719, 1796, 1845, 2108, 2704
BspQI GCTCTTC 3 cut(s) 327, 553, 2038
BspT104I TTCGAA 2 cut(s) 603, 1553
BspT107I GGYRCC 2 cut(s) 2286, 2641
BspTI CTTAAG 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 2812
BsrGI TGTACA 1 cut(s) 1769
BsrI ACTGG 8 cut(s) 298, 814, 1462, 2184, 2324, 2731, 2738, 2864
BssECI CCNNGG 8 cut(s) 112, 621, 1396, 1849, 2010, 2272, 2880, 2919
BssT1I CCWWGG 6 cut(s) 621, 1849, 2010, 2272, 2880, 2919
Bst2UI CCWGG 2 cut(s) 121, 1398
Bst4CI ACNGT 6 cut(s) 362, 581, 765, 924, 1357, 2164
Bst6I CTCTTC 5 cut(s) 178, 327, 553, 1131, 2038
BstAFI CTTAAG 1 cut(s) 349
BstAUI TGTACA 1 cut(s) 1769
BstBAI YACGTR 1 cut(s) 2602
BstBI TTCGAA 2 cut(s) 603, 1553
BstC8I GCNNGC 5 cut(s) 1565, 1992, 2489, 2656, 2912
BstDSI CCRYGG 1 cut(s) 2919
BstEII GGTNACC 2 cut(s) 590, 977
BstENI CCTNNNNNAGG 1 cut(s) 2276
BstF5I GGATG 6 cut(s) 36, 159, 1447, 1490, 2428, 2857
BstFNI CGCG 1 cut(s) 2038
BstHHI GCGC 1 cut(s) 1379
BstMAI GTCTC 1 cut(s) 193
BstMCI CGRYCG 1 cut(s) 2042
BstMWI GCNNNNNNNGC 3 cut(s) 24, 1913, 1922
BstNI CCWGG 2 cut(s) 121, 1398
BstNSI RCATGY 2 cut(s) 877, 2023
BstPI GGTNACC 2 cut(s) 590, 977
BstSCI CCNGG 7 cut(s) 39, 119, 594, 608, 665, 1180, 1396
BstSFI CTRYAG 2 cut(s) 920, 1686
BstSLI GKGCMC 1 cut(s) 2891
BstUI CGCG 1 cut(s) 2038
BstV1I GCAGC 4 cut(s) 5, 927, 1912, 2347
BstV2I GAAGAC 4 cut(s) 70, 135, 233, 2760
BstX2I RGATCY 3 cut(s) 643, 1219, 1363
BstXI CCANNNNNNTGG 5 cut(s) 150, 737, 1442, 1825, 1829
BstYI RGATCY 3 cut(s) 643, 1219, 1363
Bsu36I CCTNAGG 1 cut(s) 2666
BsuRI GGCC 9 cut(s) 69, 561, 742, 1461, 1990, 2132, 2737, 2879, 2914
BtgI CCRYGG 1 cut(s) 2919
BtgZI GCGATG 2 cut(s) 2073, 2700
BtsCI GGATG 6 cut(s) 36, 159, 1447, 1490, 2428, 2857
BtsIMutI CAGTG 8 cut(s) 291, 799, 807, 1102, 2177, 2724, 2745, 2871
BtuMI TCGCGA 1 cut(s) 2038
BveI ACCTGC 1 cut(s) 2379
Cac8I GCNNGC 5 cut(s) 1565, 1992, 2489, 2656, 2912
CfoI GCGC 1 cut(s) 1379
Cfr13I GGNCC 6 cut(s) 67, 618, 740, 1460, 1763, 1866
Csp6I GTAC 6 cut(s) 266, 531, 925, 1770, 1781, 2842
CspCI CAANNNNNGTGG 4 cut(s) 370, 405, 1510, 1545
CviQI GTAC 6 cut(s) 266, 531, 925, 1770, 1781, 2842
DraIII CACNNNGTG 1 cut(s) 2605
DriI GACNNNNNGTC 1 cut(s) 2083
Eam1104I CTCTTC 5 cut(s) 178, 327, 553, 1131, 2038
Eam1105I GACNNNNNGTC 1 cut(s) 2083
EarI CTCTTC 5 cut(s) 178, 327, 553, 1131, 2038
EciI GGCGGA 1 cut(s) 2474
Ecl136II GAGCTC 3 cut(s) 2031, 2140, 2186
Eco130I CCWWGG 6 cut(s) 621, 1849, 2010, 2272, 2880, 2919
Eco147I AGGCCT 2 cut(s) 1990, 2132
Eco24I GRGCYC 3 cut(s) 2033, 2142, 2188
Eco32I GATATC 1 cut(s) 687
Eco47I GGWCC 3 cut(s) 618, 1763, 1866
Eco53kI GAGCTC 3 cut(s) 2031, 2140, 2186
Eco57I CTGAAG 7 cut(s) 203, 227, 236, 382, 815, 1845, 2927
Eco81I CCTNAGG 1 cut(s) 2666
Eco88I CYCGRG 3 cut(s) 367, 1568, 2141
Eco91I GGTNACC 2 cut(s) 590, 977
EcoICRI GAGCTC 3 cut(s) 2031, 2140, 2186
EcoNI CCTNNNNNAGG 1 cut(s) 2276
EcoO109I RGGNCCY 1 cut(s) 1866
EcoO65I GGTNACC 2 cut(s) 590, 977
EcoRI GAATTC 1 cut(s) 841
EcoRII CCWGG 2 cut(s) 119, 1396
EcoRV GATATC 1 cut(s) 687
EcoT14I CCWWGG 6 cut(s) 621, 1849, 2010, 2272, 2880, 2919
EcoT22I ATGCAT 1 cut(s) 1975
EcoT38I GRGCYC 3 cut(s) 2033, 2142, 2188
ErhI CCWWGG 6 cut(s) 621, 1849, 2010, 2272, 2880, 2919
FalI AAGNNNNNCTT 2 cut(s) 2864, 2896
FaqI GGGAC 8 cut(s) 384, 1042, 1173, 1278, 1776, 1852, 2173, 2472
FauI CCCGC 1 cut(s) 2496
FauNDI CATATG 1 cut(s) 1887
FbaI TGATCA 2 cut(s) 2776, 2824
FblI GTMKAC 2 cut(s) 1050, 2166
Fnu4HI GCNGC 4 cut(s) 19, 916, 1926, 2336
FokI GGATG 6 cut(s) 23, 146, 1454, 1477, 2435, 2864
FriOI GRGCYC 3 cut(s) 2033, 2142, 2188
Fsp4HI GCNGC 4 cut(s) 19, 916, 1926, 2336
FspBI CTAG 6 cut(s) 374, 512, 528, 1361, 2297, 2651
GlaI GCGC 1 cut(s) 1378
GluI GCNGC 4 cut(s) 19, 916, 1926, 2336
HaeIII GGCC 9 cut(s) 69, 561, 742, 1461, 1990, 2132, 2737, 2879, 2914
HapII CCGG 9 cut(s) 41, 180, 588, 596, 609, 666, 1182, 2093, 2645
HhaI GCGC 1 cut(s) 1379
Hin6I GCGC 1 cut(s) 1377
HinP1I GCGC 1 cut(s) 1377
HindIII AAGCTT 1 cut(s) 2213
HpaII CCGG 9 cut(s) 41, 180, 588, 596, 609, 666, 1182, 2093, 2645
HphI GGTGA 9 cut(s) 185, 308, 824, 896, 989, 1740, 1874, 2400, 2776
Hpy99I CGWCG 4 cut(s) 32, 62, 137, 209
HpyAV CCTTC 7 cut(s) 111, 509, 1106, 1753, 1973, 1977, 2727
HpyCH4III ACNGT 6 cut(s) 362, 581, 765, 924, 1357, 2164
HpyCH4IV ACGT 2 cut(s) 60, 2601
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 1913, 1922
HpySE526I ACGT 2 cut(s) 60, 2601
HspAI GCGC 1 cut(s) 1377
KflI GGGWCCC 1 cut(s) 1866
Ksp22I TGATCA 2 cut(s) 2776, 2824
LguI GCTCTTC 3 cut(s) 327, 553, 2038
LmnI GCTCC 3 cut(s) 407, 1101, 2183
Lsp1109I GCAGC 4 cut(s) 5, 927, 1912, 2347
LweI GCATC 5 cut(s) 457, 533, 1982, 2063, 2322
MaeI CTAG 6 cut(s) 374, 512, 528, 1361, 2297, 2651
MaeII ACGT 2 cut(s) 60, 2601
MaeIII GTNAC 9 cut(s) 590, 816, 825, 977, 1512, 2218, 2519, 2609, 2722
MfeI CAATTG 1 cut(s) 1436
MflI RGATCY 3 cut(s) 643, 1219, 1363
MhlI GDGCHC 4 cut(s) 2033, 2142, 2188, 2891
MlyI GAGTC 9 cut(s) 131, 195, 868, 1211, 2072, 2160, 2277, 2317, 2950
MmeI TCCRAC 8 cut(s) 111, 303, 597, 963, 1061, 1197, 1299, 1924
Mph1103I ATGCAT 1 cut(s) 1975
MroXI GAANNNNTTC 2 cut(s) 630, 1638
MseI TTAA 4 cut(s) 350, 716, 1526, 1670
MslI CAYNNNNRTG 1 cut(s) 148
MspA1I CMGCKG 1 cut(s) 1925
MspCI CTTAAG 1 cut(s) 349
MspI CCGG 9 cut(s) 41, 180, 588, 596, 609, 666, 1182, 2093, 2645
MspR9I CCNGG 7 cut(s) 41, 121, 596, 610, 667, 1182, 1398
MunI CAATTG 1 cut(s) 1436
MvaI CCWGG 2 cut(s) 121, 1398
MvnI CGCG 1 cut(s) 2038
MwoI GCNNNNNNNGC 3 cut(s) 24, 1913, 1922
NciI CCSGG 5 cut(s) 41, 596, 610, 667, 1182
NcoI CCATGG 1 cut(s) 2919
NdeI CATATG 1 cut(s) 1887
NlaIV GGNNCC 7 cut(s) 118, 728, 1764, 1867, 1868, 2288, 2643
NmuCI GTSAC 5 cut(s) 825, 977, 1512, 2218, 2722
NruI TCGCGA 1 cut(s) 2038
NsiI ATGCAT 1 cut(s) 1975
NspI RCATGY 2 cut(s) 877, 2023
NspV TTCGAA 2 cut(s) 603, 1553
PaeR7I CTCGAG 1 cut(s) 1568
PceI AGGCCT 2 cut(s) 1990, 2132
PciI ACATGT 1 cut(s) 873
PciSI GCTCTTC 3 cut(s) 327, 553, 2038
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 2598, 2682
PdmI GAANNNNTTC 2 cut(s) 630, 1638
PflFI GACNNNGTC 1 cut(s) 765
PflMI CCANNNNNTGG 1 cut(s) 1334
PfoI TCCNGGA 2 cut(s) 119, 608
PkrI GCNGC 4 cut(s) 20, 917, 1927, 2337
Ple19I CGATCG 1 cut(s) 2042
PleI GAGTC 9 cut(s) 131, 194, 867, 1210, 2072, 2159, 2276, 2317, 2950
PpsI GAGTC 9 cut(s) 131, 194, 867, 1210, 2072, 2159, 2276, 2317, 2950
Ppu21I YACGTR 1 cut(s) 2602
PpuMI RGGWCCY 1 cut(s) 1866
PscI ACATGT 1 cut(s) 873
Psp124BI GAGCTC 3 cut(s) 2033, 2142, 2188
Psp5II RGGWCCY 1 cut(s) 1866
Psp6I CCWGG 2 cut(s) 119, 1396
PspEI GGTNACC 2 cut(s) 590, 977
PspGI CCWGG 2 cut(s) 119, 1396
PspN4I GGNNCC 7 cut(s) 118, 728, 1764, 1867, 1868, 2288, 2643
PspPI GGNCC 6 cut(s) 67, 618, 740, 1460, 1763, 1866
PspPPI RGGWCCY 1 cut(s) 1866
PsrI GAACNNNNNNTAC 2 cut(s) 1643, 1675
PsuI RGATCY 3 cut(s) 643, 1219, 1363
PsyI GACNNNGTC 1 cut(s) 765
PvuI CGATCG 1 cut(s) 2042
PvuII CAGCTG 1 cut(s) 1925
RruI TCGCGA 1 cut(s) 2038
RsaI GTAC 6 cut(s) 267, 532, 926, 1771, 1782, 2843
RsaNI GTAC 6 cut(s) 266, 531, 925, 1770, 1781, 2842
RseI CAYNNNNRTG 1 cut(s) 148
SacI GAGCTC 3 cut(s) 2033, 2142, 2188
SapI GCTCTTC 3 cut(s) 327, 553, 2038
SaqAI TTAA 4 cut(s) 350, 716, 1526, 1670
SatI GCNGC 4 cut(s) 19, 916, 1926, 2336
Sau96I GGNCC 6 cut(s) 67, 618, 740, 1460, 1763, 1866
ScaI AGTACT 2 cut(s) 532, 926
SchI GAGTC 9 cut(s) 131, 195, 868, 1211, 2072, 2160, 2277, 2317, 2950
ScrFI CCNGG 7 cut(s) 41, 121, 596, 610, 667, 1182, 1398
SduI GDGCHC 4 cut(s) 2033, 2142, 2188, 2891
SfaNI GCATC 5 cut(s) 457, 533, 1982, 2063, 2322
SfcI CTRYAG 2 cut(s) 920, 1686
Sfr274I CTCGAG 1 cut(s) 1568
SfuI TTCGAA 2 cut(s) 603, 1553
SinI GGWCC 3 cut(s) 618, 1763, 1866
SlaI CTCGAG 1 cut(s) 1568
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 7 cut(s) 260, 349, 1279, 1568, 1577, 2080, 2714
SmoI CTYRAG 7 cut(s) 260, 349, 1279, 1568, 1577, 2080, 2714
SpeI ACTAGT 1 cut(s) 373
SseBI AGGCCT 2 cut(s) 1990, 2132
SsiI CCGC 6 cut(s) 770, 821, 1369, 1613, 2485, 2489
SspMI CTAG 6 cut(s) 374, 512, 528, 1361, 2297, 2651
SstI GAGCTC 3 cut(s) 2033, 2142, 2188
StuI AGGCCT 2 cut(s) 1990, 2132
StyD4I CCNGG 7 cut(s) 39, 119, 594, 608, 665, 1180, 1396
StyI CCWWGG 6 cut(s) 621, 1849, 2010, 2272, 2880, 2919
TaaI ACNGT 6 cut(s) 362, 581, 765, 924, 1357, 2164
TaiI ACGT 2 cut(s) 63, 2604
TatI WGTACW 3 cut(s) 530, 924, 1769
Tru1I TTAA 4 cut(s) 350, 716, 1526, 1670
Tru9I TTAA 4 cut(s) 350, 716, 1526, 1670
TscAI CASTG 8 cut(s) 298, 799, 814, 1102, 2184, 2731, 2745, 2871
TseFI GTSAC 5 cut(s) 825, 977, 1512, 2218, 2722
TseI GCWGC 4 cut(s) 18, 915, 1925, 2335
Tsp45I GTSAC 5 cut(s) 825, 977, 1512, 2218, 2722
TspRI CASTG 8 cut(s) 298, 799, 814, 1102, 2184, 2731, 2745, 2871
Tth111I GACNNNGTC 1 cut(s) 765
Van91I CCANNNNNTGG 1 cut(s) 1334
Vha464I CTTAAG 1 cut(s) 349
VneI GTGCAC 1 cut(s) 2887
VpaK11BI GGWCC 3 cut(s) 618, 1763, 1866
XagI CCTNNNNNAGG 1 cut(s) 2276
XapI RAATTY 3 cut(s) 841, 1320, 2937
XceI RCATGY 2 cut(s) 877, 2023
XcmI CCANNNNNNNNNTGG 2 cut(s) 1829, 2926
XhoI CTCGAG 1 cut(s) 1568
XmiI GTMKAC 2 cut(s) 1050, 2166
XmnI GAANNNNTTC 2 cut(s) 630, 1638
XspI CTAG 6 cut(s) 374, 512, 528, 1361, 2297, 2651
ZrmI AGTACT 2 cut(s) 532, 926
Zsp2I ATGCAT 1 cut(s) 1975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.