Rmu_sc0002146.1_g000003

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002146.1
OG0019334
Physical Location & Seq
Forward (+)
9547 .. 10071
525 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 324 bp
atgggcaaaggaggtggttgtgtgcccagcaagaataggccaccagcaggcgtttccggcgctgatgttccgaacccaaaaagcaatgatcttgttccgggaacgctgttgtctgagatgttggagcagatgaaagtgcccaagacggatgatcatggggtgcctgtgatagtgcctgcgagactggtggaggttgatggggtgttgttcgggtttcctacccgatatgggtcaatggctgctcagttgaaggcgtttttcgactccacgggggagctgtggagggagcagaagcttgttgggttgcctgctggttctttgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

107

Amino Acids

11.23

Weight (kDa)

6.55

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 47, 228
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 2 cut(s) 277, 295
AluI AGCT 2 cut(s) 277, 295
Alw26I GTCTC 1 cut(s) 175
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 62
AsuC2I CCSGG 1 cut(s) 99
BaeGI GKGCMC 2 cut(s) 27, 141
BanI GGYRCC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 226
BccI CCATC 1 cut(s) 191
BclI TGATCA 1 cut(s) 151
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 175
BfoI RGCGCY 1 cut(s) 63
BisI GCNGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 228
Bse1I ACTGG 1 cut(s) 189
Bse3DI GCAATG 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 2 cut(s) 47, 228
BseMI GCAATG 1 cut(s) 91
BseMII CTCAG 2 cut(s) 105, 257
BseNI ACTGG 1 cut(s) 189
BseSI GKGCMC 2 cut(s) 27, 141
BseXI GCAGC 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 26
BshFI GGCC 1 cut(s) 40
BshNI GGYRCC 1 cut(s) 160
BsiSI CCGG 2 cut(s) 57, 98
BslI CCNNNNNNNGG 2 cut(s) 47, 228
BsmAI GTCTC 1 cut(s) 175
BsnI GGCC 1 cut(s) 40
Bsp1286I GDGCHC 2 cut(s) 27, 141
Bsp143I GATC 2 cut(s) 88, 151
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 2 cut(s) 106, 256
BspLI GGNNCC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 160
BsrDI GCAATG 1 cut(s) 91
BsrI ACTGG 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 2 cut(s) 88, 151
BstC8I GCNNGC 3 cut(s) 49, 177, 309
BstDEI CTNAG 2 cut(s) 114, 243
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 154
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 2 cut(s) 91, 154
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 2 cut(s) 88, 151
BstMWI GCNNNNNNNGC 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 97
BstSLI GKGCMC 2 cut(s) 27, 141
BstV1I GCAGC 1 cut(s) 226
BsuRI GGCC 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 154
Cac8I GCNNGC 3 cut(s) 49, 177, 309
CfoI GCGC 1 cut(s) 62
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 1 cut(s) 155
CviJI RGCY 4 cut(s) 40, 239, 277, 295
CviKI_1 RGCY 4 cut(s) 40, 239, 277, 295
DdeI CTNAG 2 cut(s) 114, 243
DpnI GATC 2 cut(s) 90, 153
DpnII GATC 2 cut(s) 88, 151
FaeI CATG 1 cut(s) 158
FaiI YATR 2 cut(s) 156, 228
FatI CATG 1 cut(s) 154
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 240
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 1 cut(s) 240
GlaI GCGC 1 cut(s) 61
GluI GCNGC 1 cut(s) 240
GsaI CCCAGC 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 1 cut(s) 40
HapII CCGG 2 cut(s) 57, 98
HhaI GCGC 1 cut(s) 62
Hin1II CATG 1 cut(s) 158
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 263
HpaII CCGG 2 cut(s) 57, 98
Hpy188I TCNGA 2 cut(s) 72, 115
HpyAV CCTTC 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 57
HpyF3I CTNAG 2 cut(s) 114, 243
Hsp92II CATG 1 cut(s) 158
HspAI GCGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 2 cut(s) 88, 151
LmnI GCTCC 3 cut(s) 124, 274, 286
LpnPI CCDG 9 cut(s) 33, 40, 57, 70, 111, 170, 177, 189, 297
Lsp1109I GCAGC 1 cut(s) 226
MalI GATC 2 cut(s) 90, 153
MboI GATC 2 cut(s) 88, 151
MhlI GDGCHC 2 cut(s) 27, 141
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 3 cut(s) 5, 184, 276
MspI CCGG 2 cut(s) 57, 98
MspR9I CCNGG 1 cut(s) 99
MwoI GCNNNNNNNGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 99
NdeII GATC 2 cut(s) 88, 151
NlaIII CATG 1 cut(s) 158
NlaIV GGNNCC 1 cut(s) 162
PfoI TCCNGGA 1 cut(s) 97
PkrI GCNGC 1 cut(s) 241
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PspFI CCCAGC 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 162
SatI GCNGC 1 cut(s) 240
Sau3AI GATC 2 cut(s) 88, 151
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 2 cut(s) 27, 141
SetI ASST 4 cut(s) 16, 195, 279, 297
StyD4I CCNGG 1 cut(s) 97
TaqI TCGA 1 cut(s) 261
TseI GCWGC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 146
TspGWI ACGGA 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.