Rmu_sc0002250.1_g000001

Peptidase, trypsin-like serine and cysteine proteases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002250.1
OG0004924
Physical Location & Seq
Forward (+)
3242 .. 13355
10114 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3177 bp
ctgacgaaacataatgatgatctttgttctgtcttaaacactttgcaccaagaacttctcccttactcttcctcaagctcaagctcaggcccagattttccttcagcacactatcacatggctcggtctgcactggatgaggtctcacaatctggtgcatttgtgagaagtgcttctgaattccgtaacttcatttcacgagatccgaactcgcaatttccagcagaagctgggagatatcatctgtatatatcatatgcttgcccttgggcgtcaaggtgcctggcatacttgaatattaaaggacttcagaaagctatcagctttacgtcagtcaaacctatatgggaaagaacaaaagaaagtgatgagcatatgggatgggtttttcctgcttcagataaggagctagaaggagctgaacctgaccctttgaatggagcaaaaagtgtacgagaactttatgagattgctagtacaaaatacactggaaagtacactgttccggttctatgggataaaaagcttggaacaattgtcagtaatgagagttcggagattattcgcatgtttaatactgaattcaacagcatagcagagaacccggctttggacttgtatcccgatcacttgcaatcccaagtcaatcagactaatgaatggatatacgacaagataaataacggtgtctataaatgtgggtttgccaagaagcaggagccttatgatgaggctgtgaaacagttgtatgaagctttggacagatgtgaggagatacttagcaagcaacgatacctctgtggaaacatattatctgaagctgatattcgtttgttcgtgactctcataagatttgatgaggtttatgcagtccacttcaagtgcaacaagagactcctgcgagagtacccaaatctgtttaactacaccaaggacattttccaagttcctggaatgagcaccacagtccaggtggatcacatcaaacgacactactatggaagccatccttctatcaatcccttcgggataaccggtgccgctgctgccgcagaggctccgaacggcttcggcctcgtccgggcacgccaccgacaccaagcactcggcaagtccgcggttcaggtgccggcaacccgattcccgcgcccgacccgccgggatccgacggtgacaatccaccagcgcgcggtgcagccgatcgcactccacgaatcccctggttccgccgggggccggagtttcgactcggactggaatatgatgcgcgcagaggttgtgccacgtcaccgctttgaaaatggcattgctttgagccctccatcacagctccaccattgctgttcagtagagagctacaatatggacagatccagattagatttaagatgttatcattctggatcaatacagtctgaggagtctgctctagacttggaaaggaactactgctgtcatcctaatctcccttcttcaagtccatctcctctccagccatttgcatctagtggtcagcactctgagagcaatgctgcctacttctcatggcctactttgacccggattaatgatgcagcagaagatagggcaaactattttggaaaccttcagaagggtgttcttcctgaaattttggggcggttgccatcaggacagcaggctacaaccttgcttgagctgatgaccatcagggcattccatagcaagatattgcgtcgctttagtcttggcactgccattgggtttcggattcgaaatggtgtcttgacagaaattccagctattcttgtctttgttgcccgtaaagctcacaggcattggctcagccattcacagtgtctacctgctgctcttgaggggccaggaggtgtatggtgtgatgtggatgtagtggaattttcctattttggttctccagctccaactcctaaggagcagctttacacagagcttgcagatggcttgaggggaaatgatccatatgtcggttctggttcccaggttgcaagccaggagacatatggaactatgggtgctattgttaaaagtcgaacaggaaatagacaagttggtttcctcacaaataggcatgtggcagttgatttggactatccaagccagaaaatgttccatcctatgccaccaagccttggacctggagtgtatcttggtgctgtagagagggcaacatcttttatcacagatgacctttggtatggaatatttgccggaacaaacccagaaacatttgtgcgagctgacggtgccttcattccttttgctgacgatttcaacatgaagaatgtaattacaactgtaccaggtgtgggtgagattggtgatgttaacatcatagacttgcagtctccaatcaacagtctcattggtaggcaagtgataaaagttgggagaagttcaggcttgactactgggaccatcatggcatatgccctcgagtacaatgatgaaaaagggatttgtttcttcactgatttttttgttgttggagagaatcagcagacttttgatcttgaaggtgatagtggaagcctcattctattaactggtcagaatggggagaagccacgacctgttggaatcatttggggtgggacagctaaccgtggtcgcttaaaactgaaaaaaggccaaccacctgaaaattggactagtggagttgatcttgggcgccttcttgacctcctggaactggatccgataacaaccgaagaagggcttcaagctgcaattgaagagcaacgaaatgcttcagcagctgggattggttccacagttgcagagtcatcccctgctgtctgtgctccatataaacataagctacaagataactgtggacctcttggtataaacttccgtcaagtaccatttgaaggcgaatcctatctgggaccaagtcgacgattgatgcatggtgattttcacattgaaaatggttttgaaacagctcccagtgttgagcaccagttcattccgagtttcaccagtagatctccggtaattcatagcaatcaagaggaaaatccagtatcaaaaaatgttttagcattgcggagtggttcagatgaagagatttctgtttccttacagttaggagagcctgaaccaaagagaagaaaaaactacaactctttctttagcactaaaggacccaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1058

Amino Acids

117.3

Weight (kDa)

6.18

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 87 - 193 2.9e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 235 - 315 8.9e-08 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 241 - 310 6.8e-13 Glutathione S-transferase, C-terminal domain
NAL1_N PF25608 552 - 631 6.8e-43 NARROW LEAF 1 N-terminal domain
Nal1_C PF25819 644 - 911 2.3e-156 Nal1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1852
AccB1I GGYRCC 5 cut(s) 279, 1037, 1126, 2264, 2688
AccB7I CCANNNNNTGG 1 cut(s) 2150
AccI GTMKAC 2 cut(s) 1840, 2917
AccII CGCG 5 cut(s) 1118, 1147, 1188, 1190, 1269
AclWI GGATC 9 cut(s) 197, 984, 1157, 1170, 1365, 1411, 1970, 2708, 2721
AcsI RAATTY 5 cut(s) 179, 581, 1629, 1773, 1895
AcuI CTGAAG 6 cut(s) 87, 293, 381, 837, 1592, 2754
AcyI GRCGYC 2 cut(s) 272, 2689
AfaI GTAC 7 cut(s) 453, 478, 497, 908, 2319, 2459, 2883
AgeI ACCGGT 1 cut(s) 1034
AhdI GACNNNNNGTC 1 cut(s) 2362
AhlI ACTAGT 1 cut(s) 2669
AjiI CACGTC 1 cut(s) 1286
AjuI GAANNNNNNNTTGG 4 cut(s) 593, 625, 923, 955
Alw21I GWGCWC 3 cut(s) 962, 2824, 2982
Alw26I GTCTC 5 cut(s) 148, 886, 2009, 2370, 2384
AlwI GGATC 9 cut(s) 197, 984, 1157, 1170, 1365, 1411, 1970, 2708, 2721
AlwNI CAGNNNCTG 2 cut(s) 230, 2777
Ama87I CYCGRG 1 cut(s) 2453
AoxI GGCC 6 cut(s) 88, 1072, 1234, 1547, 1859, 2647
ApeKI GCWGC 9 cut(s) 1043, 1046, 1195, 1532, 1574, 1847, 1936, 2744, 2774
ApoI RAATTY 5 cut(s) 179, 581, 1629, 1773, 1895
AseI ATTAAT 1 cut(s) 1566
AsiGI ACCGGT 1 cut(s) 1034
Asp700I GAANNNNTTC 5 cut(s) 172, 1067, 2126, 2736, 2767
AspLEI GCGC 6 cut(s) 1149, 1188, 1190, 1269, 1271, 2691
AspS9I GGNCC 8 cut(s) 89, 1234, 1859, 2153, 2433, 2855, 2909, 3167
AsuC2I CCSGG 5 cut(s) 605, 1082, 1160, 1231, 1561
AsuHPI GGTGA 7 cut(s) 1183, 1280, 2342, 2351, 2549, 2945, 2992
AsuII TTCGAA 1 cut(s) 1753
AvaI CYCGRG 1 cut(s) 2453
AvaII GGWCC 5 cut(s) 2153, 2433, 2855, 2909, 3167
AxyI CCTNAGG 1 cut(s) 1929
BaeGI GKGCMC 1 cut(s) 1087
BaeI ACNNNNGTAYC 4 cut(s) 784, 817, 2301, 2334
BamHI GGATCC 2 cut(s) 1162, 2713
BanI GGYRCC 5 cut(s) 279, 1037, 1126, 2264, 2688
BanII GRGCYC 1 cut(s) 1319
BauI CACGAG 1 cut(s) 198
Bbv12I GWGCWC 3 cut(s) 962, 2824, 2982
BbvI GCAGC 9 cut(s) 1030, 1033, 1207, 1519, 1586, 1834, 1948, 2731, 2786
BccI CCATC 9 cut(s) 375, 1014, 1330, 1489, 1654, 1694, 1952, 2139, 2444
BceAI ACGGC 1 cut(s) 1081
BciVI GTATCC 1 cut(s) 630
BcnI CCSGG 5 cut(s) 605, 1082, 1160, 1231, 1561
BcoDI GTCTC 5 cut(s) 148, 886, 2009, 2370, 2384
BcuI ACTAGT 1 cut(s) 2669
BfaI CTAG 5 cut(s) 410, 474, 1430, 1506, 2670
BfmI CTRYAG 1 cut(s) 2175
BfoI RGCGCY 1 cut(s) 2692
BfuAI ACCTGC 1 cut(s) 1852
BfuI GTATCC 1 cut(s) 630
BglI GCCNNNNNGGC 1 cut(s) 1055
BglII AGATCT 1 cut(s) 3008
BlpI GCTNAGC 1 cut(s) 1823
Bme18I GGWCC 5 cut(s) 2153, 2433, 2855, 2909, 3167
BmeRI GACNNNNNGTC 1 cut(s) 2362
BmeT110I CYCGRG 1 cut(s) 2453
BmgBI CACGTC 1 cut(s) 1286
BmgT120I GGNCC 8 cut(s) 89, 1234, 1859, 2153, 2433, 2855, 2909, 3167
BmrI ACTGGG 2 cut(s) 2439, 2964
BmsI GCATC 4 cut(s) 1254, 1511, 1561, 2916
BmuI ACTGGG 2 cut(s) 2439, 2964
BpmI CTGGAG 3 cut(s) 1475, 1899, 2178
Bpu10I CCTNAGC 1 cut(s) 85
Bpu1102I GCTNAGC 1 cut(s) 1823
Bpu14I TTCGAA 1 cut(s) 1753
BpuEI CTTGAG 5 cut(s) 58, 64, 1694, 1874, 1984
BpuMI CCSGG 5 cut(s) 605, 1082, 1160, 1231, 1561
BsaBI GATNNNNATC 1 cut(s) 1685
BsaHI GRCGYC 2 cut(s) 272, 2689
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 8 cut(s) 266, 930, 1116, 1219, 1230, 1998, 2149, 2623
BsaWI WCCGGW 3 cut(s) 505, 1034, 3013
Bse118I RCCGGY 2 cut(s) 1034, 1129
Bse21I CCTNAGG 1 cut(s) 1929
Bse3DI GCAATG 4 cut(s) 1305, 1336, 1534, 3065
Bse8I GATNNNNATC 1 cut(s) 1685
BseDI CCNNGG 8 cut(s) 266, 930, 1116, 1219, 1230, 1998, 2149, 2623
BseGI GGATG 7 cut(s) 142, 386, 1006, 1456, 1891, 2131, 2804
BseJI GATNNNNATC 1 cut(s) 1685
BseMI GCAATG 4 cut(s) 1305, 1336, 1534, 3065
BseMII CTCAG 4 cut(s) 99, 1407, 1512, 1837
BsePI GCGCGC 2 cut(s) 1186, 1267
BseRI GAGGAG 3 cut(s) 785, 1433, 1476
BseSI GKGCMC 1 cut(s) 1087
BseXI GCAGC 9 cut(s) 1030, 1033, 1207, 1519, 1586, 1834, 1948, 2731, 2786
BseYI CCCAGC 2 cut(s) 230, 2777
BsgI GTGCAG 2 cut(s) 114, 1214
Bsh1236I CGCG 5 cut(s) 1118, 1147, 1188, 1190, 1269
Bsh1285I CGRYCG 1 cut(s) 1203
BshFI GGCC 6 cut(s) 90, 1074, 1236, 1549, 1861, 2649
BshNI GGYRCC 5 cut(s) 279, 1037, 1126, 2264, 2688
BshTI ACCGGT 1 cut(s) 1034
BsiEI CGRYCG 1 cut(s) 1203
BsiHKAI GWGCWC 3 cut(s) 962, 2824, 2982
BsiHKCI CYCGRG 1 cut(s) 2453
BslFI GGGAC 3 cut(s) 2446, 2626, 2922
BsmAI GTCTC 5 cut(s) 148, 886, 2009, 2370, 2384
BsmFI GGGAC 3 cut(s) 2446, 2626, 2922
BsmI GAATGC 1 cut(s) 1694
BsnI GGCC 6 cut(s) 90, 1074, 1236, 1549, 1861, 2649
Bso31I GGTCTC 1 cut(s) 148
BsoBI CYCGRG 1 cut(s) 2453
Bsp119I TTCGAA 1 cut(s) 1753
Bsp1286I GDGCHC 5 cut(s) 962, 1087, 1319, 2824, 2982
Bsp1720I GCTNAGC 1 cut(s) 1823
BspANI GGCC 6 cut(s) 90, 1074, 1236, 1549, 1861, 2649
BspCNI CTCAG 4 cut(s) 98, 1408, 1513, 1836
BspFNI CGCG 5 cut(s) 1118, 1147, 1188, 1190, 1269
BspMI ACCTGC 1 cut(s) 1852
BspPI GGATC 9 cut(s) 197, 984, 1157, 1170, 1365, 1411, 1970, 2708, 2721
BspQI GCTCTTC 1 cut(s) 2748
BspT104I TTCGAA 1 cut(s) 1753
BspT107I GGYRCC 5 cut(s) 279, 1037, 1126, 2264, 2688
BspTNI GGTCTC 1 cut(s) 148
BsrDI GCAATG 4 cut(s) 1305, 1336, 1534, 3065
BsrFI RCCGGY 2 cut(s) 1034, 1129
BssAI RCCGGY 2 cut(s) 1034, 1129
BssECI CCNNGG 8 cut(s) 266, 930, 1116, 1219, 1230, 1998, 2149, 2623
BssHII GCGCGC 2 cut(s) 1186, 1267
BssNI GRCGYC 2 cut(s) 272, 2689
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 3 cut(s) 266, 930, 2149
Bst2BI CACGAG 1 cut(s) 198
Bst6I CTCTTC 3 cut(s) 73, 2748, 3081
BstACI GRCGYC 2 cut(s) 272, 2689
BstBI TTCGAA 1 cut(s) 1753
BstDEI CTNAG 6 cut(s) 85, 779, 1416, 1521, 1823, 1929
BstDSI CCRYGG 2 cut(s) 1116, 2623
BstENI CCTNNNNNAGG 1 cut(s) 1610
BstF5I GGATG 7 cut(s) 142, 386, 1006, 1456, 1891, 2131, 2804
BstFNI CGCG 5 cut(s) 1118, 1147, 1188, 1190, 1269
BstH2I RGCGCY 1 cut(s) 2692
BstHHI GCGC 6 cut(s) 1149, 1188, 1190, 1269, 1271, 2691
BstMAI GTCTC 5 cut(s) 148, 886, 2009, 2370, 2384
BstMCI CGRYCG 1 cut(s) 1203
BstNSI RCATGY 2 cut(s) 571, 2093
BstSFI CTRYAG 1 cut(s) 2175
BstSLI GKGCMC 1 cut(s) 1087
BstUI CGCG 5 cut(s) 1118, 1147, 1188, 1190, 1269
BstV1I GCAGC 9 cut(s) 1030, 1033, 1207, 1519, 1586, 1834, 1948, 2731, 2786
BstX2I RGATCY 5 cut(s) 202, 1162, 1370, 2713, 3008
BstXI CCANNNNNNTGG 1 cut(s) 950
BstYI RGATCY 5 cut(s) 202, 1162, 1370, 2713, 3008
Bsu36I CCTNAGG 1 cut(s) 1929
BsuI GTATCC 1 cut(s) 630
BsuRI GGCC 6 cut(s) 90, 1074, 1236, 1549, 1861, 2649
BtgI CCRYGG 2 cut(s) 1116, 2623
BtrI CACGTC 1 cut(s) 1286
BtsCI GGATG 7 cut(s) 142, 386, 1006, 1456, 1891, 2131, 2804
BtsI GCAGTG 1 cut(s) 1731
BtsIMutI CAGTG 7 cut(s) 131, 486, 498, 1731, 1841, 2487, 2977
BveI ACCTGC 1 cut(s) 1852
CaiI CAGNNNCTG 2 cut(s) 230, 2777
CfoI GCGC 6 cut(s) 1149, 1188, 1190, 1269, 1271, 2691
Cfr10I RCCGGY 2 cut(s) 1034, 1129
Cfr13I GGNCC 8 cut(s) 89, 1234, 1859, 2153, 2433, 2855, 2909, 3167
Cfr42I CCGCGG 1 cut(s) 1119
CseI GACGC 2 cut(s) 261, 1703
CsiI ACCWGGT 1 cut(s) 2320
Csp6I GTAC 7 cut(s) 452, 477, 496, 907, 2318, 2458, 2882
CspAI ACCGGT 1 cut(s) 1034
CviAII CATG 7 cut(s) 118, 568, 1545, 2090, 2296, 2440, 2930
CviQI GTAC 7 cut(s) 452, 477, 496, 907, 2318, 2458, 2882
DdeI CTNAG 6 cut(s) 85, 779, 1416, 1521, 1823, 1929
DinI GGCGCC 1 cut(s) 2690
DriI GACNNNNNGTC 1 cut(s) 2362
Eam1104I CTCTTC 3 cut(s) 73, 2748, 3081
Eam1105I GACNNNNNGTC 1 cut(s) 2362
EarI CTCTTC 3 cut(s) 73, 2748, 3081
EciI GGCGGA 1 cut(s) 1216
Eco130I CCWWGG 3 cut(s) 266, 930, 2149
Eco24I GRGCYC 1 cut(s) 1319
Eco31I GGTCTC 1 cut(s) 148
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 5 cut(s) 2153, 2433, 2855, 2909, 3167
Eco57I CTGAAG 6 cut(s) 87, 293, 381, 837, 1592, 2754
Eco81I CCTNAGG 1 cut(s) 1929
Eco88I CYCGRG 1 cut(s) 2453
EcoNI CCTNNNNNAGG 1 cut(s) 1610
EcoO109I RGGNCCY 1 cut(s) 3167
EcoRI GAATTC 2 cut(s) 179, 581
EcoRV GATATC 1 cut(s) 239
EcoT14I CCWWGG 3 cut(s) 266, 930, 2149
EcoT22I ATGCAT 1 cut(s) 2931
EcoT38I GRGCYC 1 cut(s) 1319
EgeI GGCGCC 1 cut(s) 2690
EheI GGCGCC 1 cut(s) 2690
ErhI CCWWGG 3 cut(s) 266, 930, 2149
FaeI CATG 7 cut(s) 121, 571, 1548, 2093, 2299, 2443, 2933
FalI AAGNNNNNCTT 4 cut(s) 994, 1026, 2721, 2753
FaqI GGGAC 3 cut(s) 2446, 2626, 2922
FatI CATG 7 cut(s) 117, 567, 1544, 2089, 2295, 2439, 2929
FauI CCCGC 2 cut(s) 1152, 1163
FauNDI CATATG 5 cut(s) 256, 375, 1981, 2020, 2446
FblI GTMKAC 2 cut(s) 1840, 2917
FokI GGATG 7 cut(s) 149, 393, 993, 1443, 1898, 2118, 2791
FriOI GRGCYC 1 cut(s) 1319
FspBI CTAG 5 cut(s) 410, 474, 1430, 1506, 2670
GlaI GCGC 6 cut(s) 1148, 1187, 1189, 1268, 1270, 2690
GsaI CCCAGC 2 cut(s) 234, 2781
GsuI CTGGAG 3 cut(s) 1475, 1899, 2178
HaeII RGCGCY 1 cut(s) 2692
HaeIII GGCC 6 cut(s) 90, 1074, 1236, 1549, 1861, 2649
HgaI GACGC 2 cut(s) 261, 1703
HhaI GCGC 6 cut(s) 1149, 1188, 1190, 1269, 1271, 2691
Hin1I GRCGYC 2 cut(s) 272, 2689
Hin1II CATG 7 cut(s) 121, 571, 1548, 2093, 2299, 2443, 2933
Hin6I GCGC 6 cut(s) 1147, 1186, 1188, 1267, 1269, 2689
HinP1I GCGC 6 cut(s) 1147, 1186, 1188, 1267, 1269, 2689
HincII GTYRAC 2 cut(s) 2347, 2918
HindII GTYRAC 2 cut(s) 2347, 2918
HindIII AAGCTT 2 cut(s) 524, 753
HpaI GTTAAC 1 cut(s) 2347
HphI GGTGA 7 cut(s) 1183, 1280, 2342, 2351, 2549, 2945, 2992
Hpy166II GTNNAC 7 cut(s) 452, 498, 874, 1841, 2347, 2855, 2918
Hpy8I GTNNAC 7 cut(s) 452, 498, 874, 1841, 2347, 2855, 2918
Hpy99I CGWCG 3 cut(s) 1171, 1719, 2922
HpyCH4IV ACGT 2 cut(s) 329, 1285
HpyF3I CTNAG 6 cut(s) 85, 779, 1416, 1521, 1823, 1929
HpySE526I ACGT 2 cut(s) 329, 1285
Hsp92I GRCGYC 2 cut(s) 272, 2689
Hsp92II CATG 7 cut(s) 121, 571, 1548, 2093, 2299, 2443, 2933
HspAI GCGC 6 cut(s) 1147, 1186, 1188, 1267, 1269, 2689
KasI GGCGCC 1 cut(s) 2688
KroI GCCGGC 1 cut(s) 1129
KroNI GCCGGC 1 cut(s) 1131
KspAI GTTAAC 1 cut(s) 2347
KspI CCGCGG 1 cut(s) 1119
LguI GCTCTTC 1 cut(s) 2748
Lsp1109I GCAGC 9 cut(s) 1030, 1033, 1207, 1519, 1586, 1834, 1948, 2731, 2786
LweI GCATC 4 cut(s) 1254, 1511, 1561, 2916
MabI ACCWGGT 1 cut(s) 2320
MaeI CTAG 5 cut(s) 410, 474, 1430, 1506, 2670
MaeII ACGT 2 cut(s) 329, 1285
MaeIII GTNAC 4 cut(s) 185, 838, 1171, 1286
MfeI CAATTG 2 cut(s) 534, 2748
MflI RGATCY 5 cut(s) 202, 1162, 1370, 2713, 3008
MhlI GDGCHC 5 cut(s) 962, 1087, 1319, 2824, 2982
Mly113I GGCGCC 1 cut(s) 2689
MlyI GAGTC 5 cut(s) 835, 888, 1241, 1430, 2810
MmeI TCCRAC 4 cut(s) 1190, 1946, 2485, 2575
Mph1103I ATGCAT 1 cut(s) 2931
MroNI GCCGGC 1 cut(s) 1129
MroXI GAANNNNTTC 5 cut(s) 172, 1067, 2126, 2736, 2767
MslI CAYNNNNRTG 2 cut(s) 15, 996
MspA1I CMGCKG 3 cut(s) 1043, 1118, 2777
MunI CAATTG 2 cut(s) 534, 2748
Mva1269I GAATGC 1 cut(s) 1694
MvnI CGCG 5 cut(s) 1118, 1147, 1188, 1190, 1269
NaeI GCCGGC 1 cut(s) 1131
NarI GGCGCC 1 cut(s) 2689
NciI CCSGG 5 cut(s) 605, 1082, 1160, 1231, 1561
NdeI CATATG 5 cut(s) 256, 375, 1981, 2020, 2446
NgoMIV GCCGGC 1 cut(s) 1129
NlaIII CATG 7 cut(s) 121, 571, 1548, 2093, 2299, 2443, 2933
NmeAIII GCCGAG 1 cut(s) 1086
NmuCI GTSAC 3 cut(s) 838, 1171, 1286
NsiI ATGCAT 1 cut(s) 2931
NspI RCATGY 2 cut(s) 571, 2093
NspV TTCGAA 1 cut(s) 1753
PaeR7I CTCGAG 1 cut(s) 2453
PauI GCGCGC 2 cut(s) 1186, 1267
PciSI GCTCTTC 1 cut(s) 2748
PcsI WCGNNNNNNNCGW 1 cut(s) 1209
PctI GAATGC 1 cut(s) 1694
PdiI GCCGGC 1 cut(s) 1131
PdmI GAANNNNTTC 5 cut(s) 172, 1067, 2126, 2736, 2767
PfeI GAWTC 6 cut(s) 1140, 1214, 1750, 2512, 2598, 2897
PflFI GACNNNGTC 1 cut(s) 2913
PflMI CCANNNNNTGG 1 cut(s) 2150
PfoI TCCNGGA 2 cut(s) 949, 2703
PinAI ACCGGT 1 cut(s) 1034
Ple19I CGATCG 1 cut(s) 1203
PleI GAGTC 5 cut(s) 835, 888, 1241, 1429, 2809
PluTI GGCGCC 1 cut(s) 2692
PpsI GAGTC 5 cut(s) 835, 888, 1241, 1429, 2809
PpuMI RGGWCCY 1 cut(s) 3167
PshBI ATTAAT 1 cut(s) 1566
Psp5II RGGWCCY 1 cut(s) 3167
PspFI CCCAGC 2 cut(s) 230, 2777
PspPI GGNCC 8 cut(s) 89, 1234, 1859, 2153, 2433, 2855, 2909, 3167
PspPPI RGGWCCY 1 cut(s) 3167
PspXI VCTCGAGB 1 cut(s) 2453
PsrI GAACNNNNNNTAC 2 cut(s) 535, 567
PstNI CAGNNNCTG 2 cut(s) 230, 2777
PsuI RGATCY 5 cut(s) 202, 1162, 1370, 2713, 3008
PsyI GACNNNGTC 1 cut(s) 2913
PteI GCGCGC 2 cut(s) 1186, 1267
PvuI CGATCG 1 cut(s) 1203
PvuII CAGCTG 1 cut(s) 2777
RsaI GTAC 7 cut(s) 453, 478, 497, 908, 2319, 2459, 2883
RsaNI GTAC 7 cut(s) 452, 477, 496, 907, 2318, 2458, 2882
RseI CAYNNNNRTG 2 cut(s) 15, 996
SacII CCGCGG 1 cut(s) 1119
SalI GTCGAC 1 cut(s) 2916
SapI GCTCTTC 1 cut(s) 2748
Sau96I GGNCC 8 cut(s) 89, 1234, 1859, 2153, 2433, 2855, 2909, 3167
SchI GAGTC 5 cut(s) 835, 888, 1241, 1430, 2810
SduI GDGCHC 5 cut(s) 962, 1087, 1319, 2824, 2982
SexAI ACCWGGT 1 cut(s) 2320
SfaNI GCATC 4 cut(s) 1254, 1511, 1561, 2916
SfcI CTRYAG 1 cut(s) 2175
SfoI GGCGCC 1 cut(s) 2690
Sfr274I CTCGAG 1 cut(s) 2453
Sfr303I CCGCGG 1 cut(s) 1119
SfuI TTCGAA 1 cut(s) 1753
SgrBI CCGCGG 1 cut(s) 1119
SinI GGWCC 5 cut(s) 2153, 2433, 2855, 2909, 3167
SlaI CTCGAG 1 cut(s) 2453
SmiMI CAYNNNNRTG 2 cut(s) 15, 996
SmlI CTYRAG 6 cut(s) 73, 79, 1673, 1853, 1963, 2453
SmoI CTYRAG 6 cut(s) 73, 79, 1673, 1853, 1963, 2453
SpeI ACTAGT 1 cut(s) 2669
SspDI GGCGCC 1 cut(s) 2688
SspI AATATT 2 cut(s) 298, 2223
SspMI CTAG 5 cut(s) 410, 474, 1430, 1506, 2670
StyI CCWWGG 3 cut(s) 266, 930, 2149
TaiI ACGT 2 cut(s) 332, 1288
TaqI TCGA 5 cut(s) 1245, 1753, 2050, 2454, 2917
TaqII GACCGA 1 cut(s) 114
TatI WGTACW 3 cut(s) 476, 495, 2457
TauI GCSGC 2 cut(s) 1043, 1052
TfiI GAWTC 6 cut(s) 1140, 1214, 1750, 2512, 2598, 2897
TscAI CASTG 7 cut(s) 138, 493, 505, 1738, 1841, 2494, 2977
TseFI GTSAC 3 cut(s) 838, 1171, 1286
TseI GCWGC 9 cut(s) 1043, 1046, 1195, 1532, 1574, 1847, 1936, 2744, 2774
Tsp45I GTSAC 3 cut(s) 838, 1171, 1286
TspDTI ATGAA 9 cut(s) 181, 672, 765, 2260, 2312, 2481, 2977, 3011, 3099
TspGWI ACGGA 2 cut(s) 173, 2864
TspRI CASTG 7 cut(s) 138, 493, 505, 1738, 1841, 2494, 2977
Tth111I GACNNNGTC 1 cut(s) 2913
Van91I CCANNNNNTGG 1 cut(s) 2150
VpaK11BI GGWCC 5 cut(s) 2153, 2433, 2855, 2909, 3167
VspI ATTAAT 1 cut(s) 1566
XagI CCTNNNNNAGG 1 cut(s) 1610
XapI RAATTY 5 cut(s) 179, 581, 1629, 1773, 1895
XbaI TCTAGA 1 cut(s) 1429
XceI RCATGY 2 cut(s) 571, 2093
XcmI CCANNNNNNNNNTGG 5 cut(s) 970, 1217, 1869, 2018, 2661
XhoI CTCGAG 1 cut(s) 2453
XmiI GTMKAC 2 cut(s) 1840, 2917
XmnI GAANNNNTTC 5 cut(s) 172, 1067, 2126, 2736, 2767
XspI CTAG 5 cut(s) 410, 474, 1430, 1506, 2670
Zsp2I ATGCAT 1 cut(s) 2931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.