Rmu_sc0002299.1_g000009

Belongs to the glycosyltransferase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002299.1
OG0011805
Physical Location & Seq
Forward (+)
43221 .. 48744
5524 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3177 bp
atggccgggaacgactggataaacagttacctggaggcgattctcgacgtcggtcccggcctggtcgacgatgctaagtcgtcgttgctgcttcgggagagaggtcacttcagtccgacgaggtacttcgtggaggaggtcatcaccggcttcgatgagaccgatctccaccgctcttgggttcgcgccgcggcgacgaggagtccgcaggagaggaacaccaggttggagaatctgtgctggcggatttggaacttggctcgccagaagaagcagcttgaggaaaaggaagctcagaggatgactaagcgtcgtcttgaaagagaaaggggccgcagagaagctactgctgatatgtcggaggacttatctgagggagagaagggagatgtagtggcgtctgatgtgtcggctcatggtgatagtgtcagaggcagactgccgaggattaactccgttgacgcaatggagacttttataagtcagcagaaggggaagaagctatatatcgtcttaataagtcttcatggtcttatccggggtgaaaatatggagctcggtcgcgactctgatactggtggtcaggttaaatatgtagtggaacttgcaagggctttgggtagcatgccaggagtgtatcgagttgatctgctaactagacaagtgtcatcacctgaagtagattggagttatggtgagcccacagagatgctaactccactaagtgcagaaggtttcgaggaagagacaggagagagcagtggttcttacatcattcgtataccatttggtcccagagataaatacatccccaaagaaaacctctggccacacatccccgaatttgttgatggtgcgttgaaccacgtaatacaaatgtccaaagttttaggtgaacaagtcggtggtgggaagccagtttggccagttgccatccatgggcattatgcagatgcaggtgactctgctgccctcctatctggtgctttaaatgtaccgatgcttttcactggccactcacttggcagagataagttggagcaacttttaaaacaaggccgtctatcaagggatgaaataaacgccacatacaaaataatgcgtcggatagaagctgaggagttatcccttgatgcctctgaaatagttattaccagcactagacaggagattgacgagcaatggcgcttgtatgatggattcgatcccatactggagcgaaaaatacgagccaggatcaggcgtaatgtgagctgttatggcaggttcatgccccgcatggtggtaattcctcctggaatggagttccatcatattgttccacaggatggtgatatggatggtgaaacagaggcaaacgaagaccatcatcctactcctgcagacccacctatttggactgagataatgcgcttctttaccaatcctcgaaagcctatgattcttgcccttgctagaccagatccaaaaaagaacatcacaactttggtcaaagcatttggagaatgtcgtccattaagagagctcgctaatcttacattaattatgggtaatcgagatggaattgatgacatgtcaagcacaagtgcttctgttcttctttcagttctcaagctcattgacaaacatgatttgtatgggcaagttgcataccccaaacaccacaagcagtctgatgttcctgagatatatcgtcttgcagcaaagacaaagggtgttttcatcaatccagctttcatcgagccatttggactaacattgattgaggcagctgcttatggtttacctatcgttgccacaaaaaatggcggtcctgttgatattatacaggtccttgacaatggtcttcttattgatccccatgatcagcagtctatcgccgatgctcttctgaagcttgttgcagataagcagctctgggcaagatgtcgtcagaatggattgaagaacattcacctatactcatggcctgagcactgcaagacttacctatctcgaatagccagctgcaaaacaaggcatccacagtggcaaagaagtgatgatggagcagaatcttcagaatcggattccccaagtgattccttgagagatatacaggatttgtctttgaacttgaagttctcaatggatggagaaaggagtggaattagtgtgaatgagagctctttagaatctgaaggaagtattgatagaaagagtaaaatagagaatgctgttttggcatggtcaaagggtatttcaagggacacgcggaaagctggtttctcagagaaagcagaccataacagtagtggtaagttcccagtgttgaggaggaggaagcatattattgtcattgctgtggattgtgataccattacagatctgatcgaaaccactaaaaagatttttgaggctacagaaaaggaaaggacagaaggatcaatagggtacatattgtcaacatccttgaccataatcgagatacgctcgtttctggtctctgggggcttgggccctaatgattttgatgcgtttatttgcaacagtggcagtgatctctactatccatctattaattcagatgatcgtccatttgtggttgacttttattaccactcgcacattgaatatcgctggggtggagaagggttgaggaagacgttggtacgctgggcaacttcaaccaatgataagaagagtgggagtgaggaacagattgttactgcttctgaacaactttcaaccgactattgttatgcttttaaagtgcaaaagccagcattggttccccctgtgaaggagcttcggaagtcgctgaggattcaagctttgcggtgccatgtaatttactgtcaaaatggtaacagactgaatgtgattccagtgactgcatctcgttcccaagccctcaggtatttatatttaagatggggtgtagagttatcaaagatggtggttgttgctggagaatgcggagacacagattatgaagggttgcttggagggctgcacaaaagtgtagttctgaagggggtcggcagcaatgcaatcagtcaactccacacgaaccgaaactaccctctgtcagatgtcctagcatttgacagccccaacattgttcagacaactgaaggttgtgacagcgatgatatccgcggtgccttggagaaattagaggttgtcaagaactag

Protein Analysis

1058

Amino Acids

118.53

Weight (kDa)

6.0

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GT-B_Sucrose_synth PF00862 156 - 435 1.5e-10 Sucrose synthase first GT-B domain
Glycos_transf_1 PF00534 474 - 648 1.3e-24 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 479 - 636 1.2e-15 Glycosyl transferases group 1
S6PP PF05116 838 - 1004 2.8e-12 Sucrose-6F-phosphate phosphohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011805)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20280
fragaria_vesca FvH4_1g02490 FvH4_1g02490 FvH4_1g02490
malus_domestica MD02G1022300.v1.1 MD15G1164900.v1.1
prunus_persica Prupe.7G249900_v2.0.a1
pyrus_communis pycom02g01920 pycom15g14780
rosa_chinensis RchiOBHm_Chr2g0087491
rosa_laevigata RLG00000015873
rosa_multiflora Rmu_sc0002299.1_g000009
rosa_roxburghii Rroxscaffold_2G00153510
rosa_rugosa Rorug01G0475900.1 Rorug01G0476000
rosa_samantha Rh2AG029400 Rh2BG029500 Rh2DG029400
rosa_wichuraiana Rw2G002330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AarI CACCTGC 1 cut(s) 947
AatII GACGTC 1 cut(s) 51
Acc36I ACCTGC 2 cut(s) 947, 1254
AccB1I GGYRCC 2 cut(s) 2820, 3143
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccBSI CCGCTC 1 cut(s) 174
AccI GTMKAC 2 cut(s) 66, 781
AccII CGCG 5 cut(s) 186, 191, 564, 2254, 3141
AclWI GGATC 5 cut(s) 1199, 1244, 1457, 1850, 2431
AcoI YGGCCR 4 cut(s) 3, 827, 923, 1012
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 7 cut(s) 94, 696, 1913, 2043, 2199, 3032, 3135
AcyI GRCGYC 2 cut(s) 48, 398
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 4 cut(s) 125, 996, 2435, 2652
AfiI CCNNNNNNNGG 6 cut(s) 178, 939, 1239, 1282, 1328, 2782
AflIII ACRYGT 1 cut(s) 1572
AhdI GACNNNNNGTC 2 cut(s) 201, 309
AjnI CCWGG 6 cut(s) 30, 60, 221, 628, 1232, 1294
AjuI GAANNNNNNNTTGG 6 cut(s) 209, 241, 2204, 2236, 2967, 2999
AleI CACNNNNGTG 1 cut(s) 3000
Alw21I GWGCWC 4 cut(s) 558, 1527, 1977, 2168
Alw26I GTCTC 5 cut(s) 152, 464, 740, 2488, 2955
AlwI GGATC 5 cut(s) 1199, 1244, 1457, 1850, 2431
AlwNI CAGNNNCTG 1 cut(s) 2873
AoxI GGCC 9 cut(s) 3, 58, 331, 827, 923, 1012, 1057, 1967, 2497
ApaI GGGCCC 1 cut(s) 2501
ApoI RAATTY 1 cut(s) 842
AseI ATTAAT 2 cut(s) 1541, 2559
AspLEI GCGC 3 cut(s) 188, 1188, 1413
AspS9I GGNCC 7 cut(s) 53, 331, 791, 1811, 1831, 2497, 2498
AsuC2I CCSGG 3 cut(s) 7, 57, 539
AvaII GGWCC 4 cut(s) 53, 791, 1811, 1831
AxyI CCTNAGG 1 cut(s) 2894
BaeGI GKGCMC 1 cut(s) 2501
BaeI ACNNNNGTAYC 2 cut(s) 2459, 2492
BalI TGGCCA 3 cut(s) 829, 925, 1014
BanI GGYRCC 2 cut(s) 2820, 3143
BanII GRGCYC 5 cut(s) 558, 702, 1527, 2168, 2501
BbsI GAAGAC 4 cut(s) 515, 1368, 1838, 2648
Bbv12I GWGCWC 4 cut(s) 558, 1527, 1977, 2168
BbvCI CCTCAGC 2 cut(s) 1116, 2801
BceAI ACGGC 1 cut(s) 1044
BciT130I CCWGG 6 cut(s) 32, 62, 223, 630, 1234, 1296
BclI TGATCA 1 cut(s) 1864
BcnI CCSGG 3 cut(s) 7, 57, 539
BcoDI GTCTC 5 cut(s) 152, 464, 740, 2488, 2955
BfaI CTAG 5 cut(s) 657, 1161, 1455, 3080, 3175
BfmI CTRYAG 2 cut(s) 1380, 2400
BfoI RGCGCY 1 cut(s) 1189
BfuAI ACCTGC 2 cut(s) 947, 1254
BglI GCCNNNNNGGC 1 cut(s) 922
BglII AGATCT 1 cut(s) 2365
Bme1390I CCNGG 9 cut(s) 7, 32, 57, 62, 223, 539, 630, 1234, 1296
Bme18I GGWCC 4 cut(s) 53, 791, 1811, 1831
BmeRI GACNNNNNGTC 2 cut(s) 201, 309
BmgT120I GGNCC 7 cut(s) 53, 331, 791, 1811, 1831, 2497, 2498
BmiI GGNNCC 7 cut(s) 55, 332, 793, 2499, 2772, 2822, 3145
BmrFI CCNGG 9 cut(s) 7, 32, 57, 62, 223, 539, 630, 1234, 1296
BmrI ACTGGG 1 cut(s) 2300
BmsI GCATC 9 cut(s) 61, 699, 943, 990, 1123, 1873, 2029, 2503, 2885
BmuI ACTGGG 1 cut(s) 2300
BoxI GACNNNNGTC 3 cut(s) 51, 664, 1842
BpiI GAAGAC 4 cut(s) 515, 1368, 1838, 2648
BplI GAGNNNNNCTC 2 cut(s) 2456, 2488
BpmI CTGGAG 3 cut(s) 53, 1235, 2970
Bpu10I CCTNAGC 3 cut(s) 1116, 1971, 2801
BpuEI CTTGAG 3 cut(s) 299, 1595, 2107
BpuMI CCSGG 3 cut(s) 7, 57, 539
BsaAI YACGTR 1 cut(s) 868
BsaHI GRCGYC 2 cut(s) 48, 398
BsaI GGTCTC 2 cut(s) 152, 2488
BsaJI CCNNGG 6 cut(s) 189, 443, 538, 937, 3139, 3147
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 378, 408, 679, 709
Bsc4I CCNNNNNNNGG 6 cut(s) 178, 939, 1239, 1282, 1328, 2782
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 8 cut(s) 20, 580, 917, 926, 1015, 1218, 2306, 2867
Bse21I CCTNAGG 1 cut(s) 2894
Bse3DI GCAATG 4 cut(s) 471, 1187, 2337, 3034
BseBI CCWGG 6 cut(s) 32, 62, 223, 630, 1234, 1296
BseDI CCNNGG 6 cut(s) 189, 443, 538, 937, 3139, 3147
BseLI CCNNNNNNNGG 6 cut(s) 178, 939, 1239, 1282, 1328, 2782
BseMI GCAATG 4 cut(s) 471, 1187, 2337, 3034
BseMII CTCAG 9 cut(s) 308, 363, 1107, 1392, 1674, 1962, 2283, 2792, 2908
BseNI ACTGG 8 cut(s) 20, 580, 917, 926, 1015, 1218, 2306, 2867
BseRI GAGGAG 5 cut(s) 149, 214, 1133, 2329, 2332
BseSI GKGCMC 1 cut(s) 2501
BseYI CCCAGC 2 cut(s) 2619, 2655
BsgI GTGCAG 2 cut(s) 747, 2978
Bsh1236I CGCG 5 cut(s) 186, 191, 564, 2254, 3141
Bsh1285I CGRYCG 1 cut(s) 562
BshFI GGCC 9 cut(s) 5, 60, 333, 829, 925, 1014, 1059, 1969, 2499
BshNI GGYRCC 2 cut(s) 2820, 3143
BsiEI CGRYCG 1 cut(s) 562
BsiHKAI GWGCWC 4 cut(s) 558, 1527, 1977, 2168
BsiSI CCGG 4 cut(s) 6, 57, 147, 538
BslFI GGGAC 3 cut(s) 39, 777, 2261
BslI CCNNNNNNNGG 6 cut(s) 178, 939, 1239, 1282, 1328, 2782
BsmAI GTCTC 5 cut(s) 152, 464, 740, 2488, 2955
BsmFI GGGAC 3 cut(s) 39, 777, 2261
BsmI GAATGC 2 cut(s) 2218, 2960
BsnI GGCC 9 cut(s) 5, 60, 333, 829, 925, 1014, 1059, 1969, 2499
Bso31I GGTCTC 2 cut(s) 152, 2488
Bsp120I GGGCCC 1 cut(s) 2497
Bsp1286I GDGCHC 6 cut(s) 558, 702, 1527, 1977, 2168, 2501
Bsp19I CCATGG 1 cut(s) 937
Bsp68I TCGCGA 1 cut(s) 564
BspANI GGCC 9 cut(s) 5, 60, 333, 829, 925, 1014, 1059, 1969, 2499
BspCNI CTCAG 9 cut(s) 307, 364, 1108, 1393, 1675, 1963, 2282, 2793, 2907
BspFNI CGCG 5 cut(s) 186, 191, 564, 2254, 3141
BspLI GGNNCC 7 cut(s) 55, 332, 793, 2499, 2772, 2822, 3145
BspMAI CTGCAG 1 cut(s) 1384
BspMI ACCTGC 2 cut(s) 947, 1254
BspPI GGATC 5 cut(s) 1199, 1244, 1457, 1850, 2431
BspQI GCTCTTC 1 cut(s) 1893
BspT107I GGYRCC 2 cut(s) 2820, 3143
BspTNI GGTCTC 2 cut(s) 152, 2488
BsrBI CCGCTC 1 cut(s) 174
BsrDI GCAATG 4 cut(s) 471, 1187, 2337, 3034
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 8 cut(s) 20, 580, 917, 926, 1015, 1218, 2306, 2867
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 6 cut(s) 189, 443, 538, 937, 3139, 3147
BssNAI GTATAC 1 cut(s) 782
BssNI GRCGYC 2 cut(s) 48, 398
BssT1I CCWWGG 2 cut(s) 937, 3147
Bst1107I GTATAC 1 cut(s) 782
Bst2UI CCWGG 6 cut(s) 32, 62, 223, 630, 1234, 1296
Bst4CI ACNGT 5 cut(s) 26, 2028, 2291, 2531, 2837
Bst6I CTCTTC 3 cut(s) 738, 1893, 2675
BstACI GRCGYC 2 cut(s) 48, 398
BstBAI YACGTR 1 cut(s) 868
BstC8I GCNNGC 6 cut(s) 242, 262, 626, 1527, 2005, 2763
BstDSI CCRYGG 3 cut(s) 189, 937, 3139
BstFNI CGCG 5 cut(s) 186, 191, 564, 2254, 3141
BstH2I RGCGCY 1 cut(s) 1189
BstHHI GCGC 3 cut(s) 188, 1188, 1413
BstMAI GTCTC 5 cut(s) 152, 464, 740, 2488, 2955
BstMCI CGRYCG 1 cut(s) 562
BstMWI GCNNNNNNNGC 5 cut(s) 922, 1260, 2222, 2532, 2989
BstNI CCWGG 6 cut(s) 32, 62, 223, 630, 1234, 1296
BstNSI RCATGY 2 cut(s) 628, 1576
BstPAI GACNNNNGTC 3 cut(s) 51, 664, 1842
BstSCI CCNGG 9 cut(s) 5, 30, 55, 60, 221, 537, 628, 1232, 1294
BstSFI CTRYAG 2 cut(s) 1380, 2400
BstSLI GKGCMC 1 cut(s) 2501
BstUI CGCG 5 cut(s) 186, 191, 564, 2254, 3141
BstV2I GAAGAC 4 cut(s) 515, 1368, 1838, 2648
BstX2I RGATCY 2 cut(s) 1462, 2365
BstXI CCANNNNNNTGG 2 cut(s) 1022, 1395
BstYI RGATCY 2 cut(s) 1462, 2365
BstZ17I GTATAC 1 cut(s) 782
Bsu36I CCTNAGG 1 cut(s) 2894
BsuRI GGCC 9 cut(s) 5, 60, 333, 829, 925, 1014, 1059, 1969, 2499
BtgI CCRYGG 3 cut(s) 189, 937, 3139
BtgZI GCGATG 1 cut(s) 3144
BtsI GCAGTG 3 cut(s) 766, 1975, 2542
BtsIMutI CAGTG 8 cut(s) 766, 1008, 1975, 2033, 2313, 2536, 2542, 2874
BtuMI TCGCGA 1 cut(s) 564
BveI ACCTGC 2 cut(s) 947, 1254
Cac8I GCNNGC 6 cut(s) 242, 262, 626, 1527, 2005, 2763
CaiI CAGNNNCTG 1 cut(s) 2873
CfoI GCGC 3 cut(s) 188, 1188, 1413
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 7 cut(s) 53, 331, 791, 1811, 1831, 2497, 2498
Cfr42I CCGCGG 2 cut(s) 192, 3142
CseI GACGC 4 cut(s) 299, 387, 470, 1091
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 4 cut(s) 124, 995, 2434, 2651
CspCI CAANNNNNGTGG 4 cut(s) 158, 193, 2919, 2954
CviQI GTAC 4 cut(s) 124, 995, 2434, 2651
DraI TTTAAA 3 cut(s) 990, 1050, 2749
DraIII CACNNNGTG 1 cut(s) 725
DriI GACNNNNNGTC 2 cut(s) 201, 309
EaeI YGGCCR 4 cut(s) 3, 827, 923, 1012
Eam1104I CTCTTC 3 cut(s) 738, 1893, 2675
Eam1105I GACNNNNNGTC 2 cut(s) 201, 309
EarI CTCTTC 3 cut(s) 738, 1893, 2675
EciI GGCGGA 1 cut(s) 259
Ecl136II GAGCTC 3 cut(s) 556, 1525, 2166
Eco130I CCWWGG 2 cut(s) 937, 3147
Eco24I GRGCYC 5 cut(s) 558, 702, 1527, 2168, 2501
Eco31I GGTCTC 2 cut(s) 152, 2488
Eco32I GATATC 1 cut(s) 3136
Eco47I GGWCC 4 cut(s) 53, 791, 1811, 1831
Eco53kI GAGCTC 3 cut(s) 556, 1525, 2166
Eco57I CTGAAG 7 cut(s) 94, 696, 1913, 2043, 2199, 3032, 3135
Eco81I CCTNAGG 1 cut(s) 2894
EcoICRI GAGCTC 3 cut(s) 556, 1525, 2166
EcoO109I RGGNCCY 2 cut(s) 1831, 2498
EcoRII CCWGG 6 cut(s) 30, 60, 221, 628, 1232, 1294
EcoRV GATATC 1 cut(s) 3136
EcoT14I CCWWGG 2 cut(s) 937, 3147
EcoT38I GRGCYC 5 cut(s) 558, 702, 1527, 2168, 2501
ErhI CCWWGG 2 cut(s) 937, 3147
FalI AAGNNNNNCTT 2 cut(s) 2967, 2999
FaqI GGGAC 3 cut(s) 39, 777, 2261
FauI CCCGC 1 cut(s) 1283
FbaI TGATCA 1 cut(s) 1864
FblI GTMKAC 2 cut(s) 66, 781
FriOI GRGCYC 5 cut(s) 558, 702, 1527, 2168, 2501
FspBI CTAG 5 cut(s) 657, 1161, 1455, 3080, 3175
GlaI GCGC 3 cut(s) 187, 1187, 1412
GsaI CCCAGC 2 cut(s) 2623, 2659
GsuI CTGGAG 3 cut(s) 53, 1235, 2970
HaeII RGCGCY 1 cut(s) 1189
HaeIII GGCC 9 cut(s) 5, 60, 333, 829, 925, 1014, 1059, 1969, 2499
HapII CCGG 4 cut(s) 6, 57, 147, 538
HgaI GACGC 4 cut(s) 299, 387, 470, 1091
HhaI GCGC 3 cut(s) 188, 1188, 1413
Hin1I GRCGYC 2 cut(s) 48, 398
Hin6I GCGC 3 cut(s) 186, 1186, 1411
HinP1I GCGC 3 cut(s) 186, 1186, 1411
HincII GTYRAC 5 cut(s) 67, 460, 2445, 2587, 3041
HindII GTYRAC 5 cut(s) 67, 460, 2445, 2587, 3041
HindIII AAGCTT 2 cut(s) 1895, 2811
HpaII CCGG 4 cut(s) 6, 57, 147, 538
Hpy166II GTNNAC 8 cut(s) 67, 460, 782, 896, 1784, 2445, 2587, 3041
Hpy188III TCNNGA 9 cut(s) 44, 95, 317, 563, 1556, 1682, 1995, 2464, 3169
Hpy8I GTNNAC 8 cut(s) 67, 460, 782, 896, 1784, 2445, 2587, 3041
Hpy99I CGWCG 8 cut(s) 50, 53, 71, 85, 121, 199, 315, 1107
HpyCH4III ACNGT 5 cut(s) 26, 2028, 2291, 2531, 2837
HpyCH4IV ACGT 3 cut(s) 48, 867, 2645
HpyF10VI GCNNNNNNNGC 5 cut(s) 922, 1260, 2222, 2532, 2989
HpySE526I ACGT 3 cut(s) 48, 867, 2645
Hsp92I GRCGYC 2 cut(s) 48, 398
HspAI GCGC 3 cut(s) 186, 1186, 1411
Ksp22I TGATCA 1 cut(s) 1864
KspI CCGCGG 2 cut(s) 192, 3142
LguI GCTCTTC 1 cut(s) 1893
LmnI GCTCC 5 cut(s) 553, 1039, 1216, 2048, 2785
LweI GCATC 9 cut(s) 61, 699, 943, 990, 1123, 1873, 2029, 2503, 2885
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 5 cut(s) 657, 1161, 1455, 3080, 3175
MaeII ACGT 3 cut(s) 48, 867, 2645
MaeIII GTNAC 7 cut(s) 26, 104, 959, 2704, 2846, 2869, 3122
MbiI CCGCTC 1 cut(s) 174
MflI RGATCY 2 cut(s) 1462, 2365
MhlI GDGCHC 6 cut(s) 558, 702, 1527, 1977, 2168, 2501
MlsI TGGCCA 3 cut(s) 829, 925, 1014
MluCI AATT 8 cut(s) 842, 1287, 1542, 1563, 2148, 2560, 2829, 3155
MluNI TGGCCA 3 cut(s) 829, 925, 1014
MlyI GAGTC 3 cut(s) 211, 560, 956
MmeI TCCRAC 5 cut(s) 140, 207, 339, 1017, 1085
Mox20I TGGCCA 3 cut(s) 829, 925, 1014
MscI TGGCCA 3 cut(s) 829, 925, 1014
MslI CAYNNNNRTG 3 cut(s) 707, 2342, 3000
Msp20I TGGCCA 3 cut(s) 829, 925, 1014
MspA1I CMGCKG 4 cut(s) 191, 1772, 2007, 3141
MspI CCGG 4 cut(s) 6, 57, 147, 538
MspR9I CCNGG 9 cut(s) 7, 32, 57, 62, 223, 539, 630, 1234, 1296
Mva1269I GAATGC 2 cut(s) 2218, 2960
MvaI CCWGG 6 cut(s) 32, 62, 223, 630, 1234, 1296
MvnI CGCG 5 cut(s) 186, 191, 564, 2254, 3141
MwoI GCNNNNNNNGC 5 cut(s) 922, 1260, 2222, 2532, 2989
NciI CCSGG 3 cut(s) 7, 57, 539
NcoI CCATGG 1 cut(s) 937
NlaIV GGNNCC 7 cut(s) 55, 332, 793, 2499, 2772, 2822, 3145
NmeAIII GCCGAG 1 cut(s) 468
NmuCI GTSAC 4 cut(s) 104, 959, 2869, 3122
NruI TCGCGA 1 cut(s) 564
NspI RCATGY 2 cut(s) 628, 1576
OliI CACNNNNGTG 1 cut(s) 3000
PaeI GCATGC 1 cut(s) 628
PaqCI CACCTGC 1 cut(s) 947
PciI ACATGT 1 cut(s) 1572
PciSI GCTCTTC 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 2 cut(s) 2218, 2960
PflMI CCANNNNNTGG 1 cut(s) 1328
PfoI TCCNGGA 1 cut(s) 1294
PleI GAGTC 3 cut(s) 210, 560, 956
PpsI GAGTC 3 cut(s) 210, 560, 956
Ppu21I YACGTR 1 cut(s) 868
PpuMI RGGWCCY 1 cut(s) 1831
PscI ACATGT 1 cut(s) 1572
PshAI GACNNNNGTC 3 cut(s) 51, 664, 1842
PshBI ATTAAT 2 cut(s) 1541, 2559
PsiI TTATAA 1 cut(s) 479
Psp124BI GAGCTC 3 cut(s) 558, 1527, 2168
Psp5II RGGWCCY 1 cut(s) 1831
Psp6I CCWGG 6 cut(s) 30, 60, 221, 628, 1232, 1294
PspFI CCCAGC 2 cut(s) 2619, 2655
PspGI CCWGG 6 cut(s) 30, 60, 221, 628, 1232, 1294
PspN4I GGNNCC 7 cut(s) 55, 332, 793, 2499, 2772, 2822, 3145
PspOMI GGGCCC 1 cut(s) 2497
PspPI GGNCC 7 cut(s) 53, 331, 791, 1811, 1831, 2497, 2498
PspPPI RGGWCCY 1 cut(s) 1831
PsrI GAACNNNNNNTAC 2 cut(s) 3044, 3076
PstI CTGCAG 1 cut(s) 1384
PstNI CAGNNNCTG 1 cut(s) 2873
PsuI RGATCY 2 cut(s) 1462, 2365
PvuII CAGCTG 2 cut(s) 1772, 2007
RruI TCGCGA 1 cut(s) 564
RsaI GTAC 4 cut(s) 125, 996, 2435, 2652
RsaNI GTAC 4 cut(s) 124, 995, 2434, 2651
RseI CAYNNNNRTG 3 cut(s) 707, 2342, 3000
SacI GAGCTC 3 cut(s) 558, 1527, 2168
SacII CCGCGG 2 cut(s) 192, 3142
SalI GTCGAC 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 1893
Sau96I GGNCC 7 cut(s) 53, 331, 791, 1811, 1831, 2497, 2498
SchI GAGTC 3 cut(s) 211, 560, 956
ScrFI CCNGG 9 cut(s) 7, 32, 57, 62, 223, 539, 630, 1234, 1296
SduI GDGCHC 6 cut(s) 558, 702, 1527, 1977, 2168, 2501
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 9 cut(s) 61, 699, 943, 990, 1123, 1873, 2029, 2503, 2885
SfcI CTRYAG 2 cut(s) 1380, 2400
Sfr303I CCGCGG 2 cut(s) 192, 3142
SgrBI CCGCGG 2 cut(s) 192, 3142
SinI GGWCC 4 cut(s) 53, 791, 1811, 1831
SmiMI CAYNNNNRTG 3 cut(s) 707, 2342, 3000
SmlI CTYRAG 3 cut(s) 278, 1610, 2086
SmoI CTYRAG 3 cut(s) 278, 1610, 2086
SphI GCATGC 1 cut(s) 628
Sse9I AATT 8 cut(s) 842, 1287, 1542, 1563, 2148, 2560, 2829, 3155
SspMI CTAG 5 cut(s) 657, 1161, 1455, 3080, 3175
SstI GAGCTC 3 cut(s) 558, 1527, 2168
StyD4I CCNGG 9 cut(s) 5, 30, 55, 60, 221, 537, 628, 1232, 1294
StyI CCWWGG 2 cut(s) 937, 3147
TaaI ACNGT 5 cut(s) 26, 2028, 2291, 2531, 2837
TaiI ACGT 3 cut(s) 51, 870, 2648
TaqII GACCGA 3 cut(s) 41, 176, 548
TasI AATT 8 cut(s) 842, 1287, 1542, 1563, 2148, 2560, 2829, 3155
TauI GCSGC 3 cut(s) 191, 194, 336
TscAI CASTG 8 cut(s) 766, 1015, 1982, 2033, 2313, 2536, 2542, 2874
TseFI GTSAC 4 cut(s) 104, 959, 2869, 3122
Tsp45I GTSAC 4 cut(s) 104, 959, 2869, 3122
TspDTI ATGAA 6 cut(s) 515, 1089, 1258, 1711, 1726, 2988
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 8 cut(s) 766, 1015, 1982, 2033, 2313, 2536, 2542, 2874
Van91I CCANNNNNTGG 1 cut(s) 1328
VpaK11BI GGWCC 4 cut(s) 53, 791, 1811, 1831
VspI ATTAAT 2 cut(s) 1541, 2559
XapI RAATTY 1 cut(s) 842
XceI RCATGY 2 cut(s) 628, 1576
XcmI CCANNNNNNNNNTGG 1 cut(s) 2291
XmiI GTMKAC 2 cut(s) 66, 781
XspI CTAG 5 cut(s) 657, 1161, 1455, 3080, 3175
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.