Rmu_sc0002769.1_g000011

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002769.1
OG0020605
Physical Location & Seq
Reverse (-)
50303 .. 52800
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002769.1_g000011.1.cds

Sequence Viewer

Length: 924 bp
atgggcatagagctcataacttacgcagtcagtggcaagacgaatatccaagcaagagagggcgtgatgaaaggccaggagggttctttcgctatgatcccgtccactgggcagaaagccatcatcaaagggaggagggaaacacctagaaaggactatttttgggtccggcatctctcccagcaagtacaagtaaataaaacactcggggtagagaggagctcgataccttacgttgcggcaaagctagttccccagaagggaatcgactggatgttcctttggaatatcatcgtgatggaagcgagggaaataaatctgcagccaaaaggcaagaatccagaaaaggccactaatgtcgagagcagtggccagagaattccaaagcccagtaggtttgttggaagaaatgcaaaagataaatttgcaaagccagaattcaagaatgtgatacctcctgtatggctagaagcccactttcctccttggtccccaactgctaaaatcaccaggcacctgccgaagagccgctatgggactacggatgggaagcccgtaataggccatagcatcatctggcaaacgatcgcgaggtgtaggacccagacacgcatgactgtcatcgctaccaaagcccatcagtctacttctctcctttccgatttcagcaatgtttttgttcaagatttccagaatagattttgttcttctggttctattaatttgaattttgctggcaactttgtcaatattattaagcttgtaattgataatgcagataatgatggtttgcttagagatatcacattggaggaaattcgtttgacaatcaactcaattggaggtcttgaagcacctggtccagatggtatccatgcaatattctaccaaaagcattggaaaattgtcaaagagaagatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

307

Amino Acids

34.56

Weight (kDa)

9.94

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020605)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0205481
rosa_multiflora Rmu_co8129578.1_g000001 Rmu_sc0002769.1_g000011 Rmu_sc0004080.1_g000010 Rmu_sc0010099.1_g000004
rosa_samantha Rh5DG183700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 513
AccI GTMKAC 1 cut(s) 644
AccII CGCG 1 cut(s) 590
AciI CCGC 2 cut(s) 239, 529
AclWI GGATC 1 cut(s) 91
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 5 cut(s) 378, 422, 437, 727, 816
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 3 cut(s) 107, 260, 560
AgsI TTSAA 4 cut(s) 442, 683, 727, 851
AjnI CCWGG 3 cut(s) 75, 509, 856
AluBI AGCT 4 cut(s) 13, 222, 247, 760
AluI AGCT 4 cut(s) 13, 222, 247, 760
Alw21I GWGCWC 2 cut(s) 15, 224
AlwI GGATC 1 cut(s) 91
Ama87I CYCGRG 1 cut(s) 206
AoxI GGCC 4 cut(s) 73, 348, 370, 562
ApeKI GCWGC 1 cut(s) 322
ApoI RAATTY 5 cut(s) 378, 422, 437, 727, 816
AseI ATTAAT 1 cut(s) 720
AspS9I GGNCC 4 cut(s) 166, 489, 600, 860
AsuHPI GGTGA 1 cut(s) 499
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 4 cut(s) 166, 489, 600, 860
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 513
BanII GRGCYC 2 cut(s) 15, 224
Bbv12I GWGCWC 2 cut(s) 15, 224
BbvI GCAGC 1 cut(s) 334
BccI CCATC 6 cut(s) 128, 292, 539, 645, 779, 860
BciT130I CCWGG 3 cut(s) 77, 511, 858
BciVI GTATCC 1 cut(s) 881
BfaI CTAG 3 cut(s) 147, 248, 467
BfmI CTRYAG 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 525
BfuI GTATCC 1 cut(s) 881
BisI GCNGC 3 cut(s) 240, 323, 529
BlsI GCNGC 3 cut(s) 241, 324, 530
Bme1390I CCNGG 3 cut(s) 77, 511, 858
Bme18I GGWCC 4 cut(s) 166, 489, 600, 860
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 4 cut(s) 166, 489, 600, 860
BmiI GGNNCC 4 cut(s) 167, 491, 515, 602
BmrFI CCNGG 3 cut(s) 77, 511, 858
BmrI ACTGGG 2 cut(s) 117, 384
BmsI GCATC 2 cut(s) 181, 579
BmuI ACTGGG 2 cut(s) 117, 384
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BsaJI CCNNGG 1 cut(s) 485
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 260, 560
Bse1I ACTGG 3 cut(s) 112, 275, 390
Bse3DI GCAATG 1 cut(s) 676
BseBI CCWGG 3 cut(s) 77, 511, 858
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 279, 550
BseLI CCNNNNNNNGG 3 cut(s) 107, 260, 560
BseMI GCAATG 1 cut(s) 676
BseNI ACTGG 3 cut(s) 112, 275, 390
BseRI GAGGAG 2 cut(s) 148, 232
BseXI GCAGC 1 cut(s) 334
BseYI CCCAGC 1 cut(s) 180
Bsh1236I CGCG 1 cut(s) 590
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 4 cut(s) 75, 350, 372, 564
BshNI GGYRCC 1 cut(s) 513
BsiEI CGRYCG 1 cut(s) 588
BsiHKAI GWGCWC 2 cut(s) 15, 224
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 1 cut(s) 169
BslFI GGGAC 2 cut(s) 475, 550
BslI CCNNNNNNNGG 3 cut(s) 107, 260, 560
BsmFI GGGAC 2 cut(s) 475, 550
BsnI GGCC 4 cut(s) 75, 350, 372, 564
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 15, 224
Bsp143I GATC 2 cut(s) 96, 585
Bsp68I TCGCGA 1 cut(s) 590
BspACI CCGC 2 cut(s) 239, 529
BspANI GGCC 4 cut(s) 75, 350, 372, 564
BspFNI CGCG 1 cut(s) 590
BspLI GGNNCC 4 cut(s) 167, 491, 515, 602
BspMAI CTGCAG 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 525
BspPI GGATC 1 cut(s) 91
BspQI GCTCTTC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 676
BsrI ACTGG 3 cut(s) 112, 275, 390
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 2 cut(s) 96, 585
BssT1I CCWWGG 1 cut(s) 485
Bst2UI CCWGG 3 cut(s) 77, 511, 858
Bst4CI ACNGT 1 cut(s) 619
Bst6I CTCTTC 1 cut(s) 518
BstC8I GCNNGC 1 cut(s) 736
BstDEI CTNAG 1 cut(s) 794
BstF5I GGATG 2 cut(s) 279, 550
BstFNI CGCG 1 cut(s) 590
BstKTI GATC 2 cut(s) 99, 588
BstMBI GATC 2 cut(s) 96, 585
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 3 cut(s) 77, 511, 858
BstSCI CCNGG 3 cut(s) 75, 509, 856
BstSFI CTRYAG 1 cut(s) 320
BstUI CGCG 1 cut(s) 590
BstV1I GCAGC 1 cut(s) 334
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 4 cut(s) 75, 350, 372, 564
BtgZI GCGATG 1 cut(s) 607
BtsCI GGATG 2 cut(s) 279, 550
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 3 cut(s) 37, 105, 373
BtuMI TCGCGA 1 cut(s) 590
BveI ACCTGC 1 cut(s) 525
Cac8I GCNNGC 1 cut(s) 736
Cfr13I GGNCC 4 cut(s) 166, 489, 600, 860
CsiI ACCWGGT 1 cut(s) 856
Csp6I GTAC 1 cut(s) 188
CviAII CATG 2 cut(s) 613, 875
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 1 cut(s) 794
DpnI GATC 2 cut(s) 98, 587
DpnII GATC 2 cut(s) 96, 585
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
Ecl136II GAGCTC 2 cut(s) 13, 222
Eco130I CCWWGG 1 cut(s) 485
Eco24I GRGCYC 2 cut(s) 15, 224
Eco32I GATATC 1 cut(s) 802
Eco47I GGWCC 4 cut(s) 166, 489, 600, 860
Eco53kI GAGCTC 2 cut(s) 13, 222
Eco88I CYCGRG 1 cut(s) 206
EcoICRI GAGCTC 2 cut(s) 13, 222
EcoO109I RGGNCCY 1 cut(s) 600
EcoRI GAATTC 2 cut(s) 378, 437
EcoRII CCWGG 3 cut(s) 75, 509, 856
EcoRV GATATC 1 cut(s) 802
EcoT14I CCWWGG 1 cut(s) 485
EcoT38I GRGCYC 2 cut(s) 15, 224
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 2 cut(s) 616, 878
FaiI YATR 9 cut(s) 8, 17, 95, 463, 534, 567, 614, 876, 922
FaqI GGGAC 2 cut(s) 475, 550
FatI CATG 2 cut(s) 612, 874
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 3 cut(s) 240, 323, 529
FokI GGATG 2 cut(s) 286, 557
FriOI GRGCYC 2 cut(s) 15, 224
Fsp4HI GCNGC 3 cut(s) 240, 323, 529
FspBI CTAG 3 cut(s) 147, 248, 467
GluI GCNGC 3 cut(s) 240, 323, 529
GsaI CCCAGC 1 cut(s) 184
HaeIII GGCC 4 cut(s) 75, 350, 372, 564
HapII CCGG 1 cut(s) 169
Hin1II CATG 2 cut(s) 616, 878
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 2 cut(s) 264, 337
HpaII CCGG 1 cut(s) 169
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 2 cut(s) 105, 645
Hpy188I TCNGA 1 cut(s) 661
Hpy188III TCNNGA 9 cut(s) 295, 341, 361, 442, 589, 683, 691, 848, 863
Hpy8I GTNNAC 2 cut(s) 105, 645
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 5 cut(s) 322, 413, 428, 776, 878
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 1 cut(s) 794
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 2 cut(s) 616, 878
Kzo9I GATC 2 cut(s) 96, 585
LguI GCTCTTC 1 cut(s) 518
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 1 cut(s) 334
LweI GCATC 2 cut(s) 181, 579
MabI ACCWGGT 1 cut(s) 856
MaeI CTAG 3 cut(s) 147, 248, 467
MaeII ACGT 1 cut(s) 234
MalI GATC 2 cut(s) 98, 587
MboI GATC 2 cut(s) 96, 585
MboII GAAGA 3 cut(s) 417, 535, 699
MfeI CAATTG 1 cut(s) 837
MhlI GDGCHC 2 cut(s) 15, 224
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 9 cut(s) 378, 422, 437, 721, 727, 765, 816, 837, 903
MluNI TGGCCA 1 cut(s) 372
MmeI TCCRAC 1 cut(s) 382
Mox20I TGGCCA 1 cut(s) 372
MscI TGGCCA 1 cut(s) 372
MseI TTAA 2 cut(s) 720, 756
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 372
MspI CCGG 1 cut(s) 169
MspR9I CCNGG 3 cut(s) 77, 511, 858
MunI CAATTG 1 cut(s) 837
MvaI CCWGG 3 cut(s) 77, 511, 858
MvnI CGCG 1 cut(s) 590
MwoI GCNNNNNNNGC 1 cut(s) 632
NdeII GATC 2 cut(s) 96, 585
NlaIII CATG 2 cut(s) 616, 878
NlaIV GGNNCC 4 cut(s) 167, 491, 515, 602
NruI TCGCGA 1 cut(s) 590
PaqCI CACCTGC 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 264, 337
PkrI GCNGC 3 cut(s) 241, 324, 530
Ple19I CGATCG 1 cut(s) 588
PpuMI RGGWCCY 1 cut(s) 600
PshBI ATTAAT 1 cut(s) 720
Psp124BI GAGCTC 2 cut(s) 15, 224
Psp5II RGGWCCY 1 cut(s) 600
Psp6I CCWGG 3 cut(s) 75, 509, 856
PspFI CCCAGC 1 cut(s) 180
PspGI CCWGG 3 cut(s) 75, 509, 856
PspN4I GGNNCC 4 cut(s) 167, 491, 515, 602
PspPI GGNCC 4 cut(s) 166, 489, 600, 860
PspPPI RGGWCCY 1 cut(s) 600
PstI CTGCAG 1 cut(s) 324
PvuI CGATCG 1 cut(s) 588
RruI TCGCGA 1 cut(s) 590
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 296
SacI GAGCTC 2 cut(s) 15, 224
SapI GCTCTTC 1 cut(s) 518
SaqAI TTAA 2 cut(s) 720, 756
SatI GCNGC 3 cut(s) 240, 323, 529
Sau3AI GATC 2 cut(s) 96, 585
Sau96I GGNCC 4 cut(s) 166, 489, 600, 860
ScrFI CCNGG 3 cut(s) 77, 511, 858
SduI GDGCHC 2 cut(s) 15, 224
SexAI ACCWGGT 1 cut(s) 856
SfaNI GCATC 2 cut(s) 181, 579
SfcI CTRYAG 1 cut(s) 320
SinI GGWCC 4 cut(s) 166, 489, 600, 860
SmiMI CAYNNNNRTG 1 cut(s) 296
Sse9I AATT 9 cut(s) 378, 422, 437, 721, 727, 765, 816, 837, 903
SsiI CCGC 2 cut(s) 239, 529
SspI AATATT 2 cut(s) 751, 882
SspMI CTAG 3 cut(s) 147, 248, 467
SstI GAGCTC 2 cut(s) 15, 224
StyD4I CCNGG 3 cut(s) 75, 509, 856
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 1 cut(s) 237
TaqI TCGA 3 cut(s) 224, 267, 360
TasI AATT 9 cut(s) 378, 422, 437, 721, 727, 765, 816, 837, 903
TatI WGTACW 1 cut(s) 187
TauI GCSGC 2 cut(s) 242, 531
TfiI GAWTC 2 cut(s) 264, 337
Tru1I TTAA 2 cut(s) 720, 756
Tru9I TTAA 2 cut(s) 720, 756
TscAI CASTG 3 cut(s) 37, 112, 373
TseI GCWGC 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 3 cut(s) 37, 112, 373
VpaK11BI GGWCC 4 cut(s) 166, 489, 600, 860
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 5 cut(s) 378, 422, 437, 727, 816
XmiI GTMKAC 1 cut(s) 644
XspI CTAG 3 cut(s) 147, 248, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.