Rmu_sc0003168.1_g000012

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003168.1
OG0016040
Physical Location & Seq
Forward (+)
68126 .. 69787
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
atgtcaagtcaagaaaatggctcatctgaattcccaacttggccggagtacaaacttccggatttgttatctactgaaagcgttcgacaagtccatgcagcgattgaaagcgaatgggatcctcttcaacggtctgcgtgccaaacagcagctggaagagccttgtggaagcatgtggtcaacgacccgctggcagacatacttgcaggagagacgtacctaaaatctctccatgagaagataaagaaagactgcgtcaacaaggcacgagaaatctctggggtgattcttgcagttcgaacactctggtttgattcaaaacttgaagcagcactccattccttcgacaacagagaagcacaagtagttctccttggtgcaggaatggatgcaagggcttaccgattgagttgcttgaaggaaagtaatgtgtttgaagtagattttcctgacgtcttgcaaatcaaagctactcttctgcaagcagcgatggattctacaaatgatcaccagtatctaatgatgacagcaaaatccataaccagagttgaggctgacatcagaagcgatgactggcttgaaaaacttcaaatatcaggatttatgccagagaagaacacagtatgggttctggaaggtatcttgtactacctaacccaccctcaggccacggaagtattgaaaatcatagcagacaagtgctctcttacgcaaacagtactcttggcagatttcatgaacaaaccatcaaccagtctttccagctccaactttcatttctacagtgattggcccgatcatctccttccatctctcggcttttctcatgttaaactttcacaaattggtgatccagatgctcattttgggctcctgcacgatcccttaaatctgttcaacaaactccgcagcttgcctagatcagtacaaacaaacccagatgatggaacaccatgttgtcgcttgtatttggttgaggcttctggttcaccaaagcaaaatattccatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LCM

Protein Analysis

336

Amino Acids

37.67

Weight (kDa)

5.46

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 46 - 230 4.2e-41 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42760 AT5G42760
fragaria_vesca FvH4_2g39790
malus_domestica MD15G1027400.v1.1
prunus_persica Prupe.1G379200_v2.0.a1
pyrus_communis pycom15g02340
rosa_laevigata RLG00000011280
rosa_multiflora Rmu_sc0003168.1_g000012 Rmu_sc0035551.1_g000003
rosa_roxburghii Rroxscaffold_7G00167210
rosa_rugosa Rorug06G0300600
rosa_samantha Rh6AG414400 Rh6BG421300 Rh6CG428900 Rh6DG414300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 456
AccB7I CCANNNNNTGG 1 cut(s) 944
AccIII TCCGGA 1 cut(s) 58
AciI CCGC 2 cut(s) 188, 907
AclWI GGATC 4 cut(s) 113, 126, 845, 875
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 5 cut(s) 50, 218, 647, 720, 927
AfiI CCNNNNNNNGG 3 cut(s) 664, 815, 944
AjuI GAANNNNNNNTTGG 2 cut(s) 761, 793
AluBI AGCT 4 cut(s) 152, 470, 765, 912
AluI AGCT 4 cut(s) 152, 470, 765, 912
Alw21I GWGCWC 1 cut(s) 704
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 4 cut(s) 113, 126, 845, 875
AlwNI CAGNNNCTG 1 cut(s) 152
Aor13HI TCCGGA 1 cut(s) 58
AoxI GGCC 3 cut(s) 41, 666, 791
ApeKI GCWGC 5 cut(s) 98, 149, 329, 485, 909
ApoI RAATTY 1 cut(s) 29
Asp700I GAANNNNTTC 2 cut(s) 81, 585
AspS9I GGNCC 1 cut(s) 792
AsuHPI GGTGA 4 cut(s) 295, 500, 860, 981
AsuII TTCGAA 1 cut(s) 298
AxyI CCTNAGG 1 cut(s) 663
BamHI GGATCC 1 cut(s) 118
BanII GRGCYC 1 cut(s) 873
BauI CACGAG 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 704
BbvI GCAGC 5 cut(s) 110, 161, 341, 497, 921
BccI CCATC 4 cut(s) 484, 754, 817, 938
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 1 cut(s) 918
BfmI CTRYAG 1 cut(s) 781
BisI GCNGC 5 cut(s) 99, 150, 330, 486, 910
BlsI GCNGC 5 cut(s) 100, 151, 331, 487, 911
BmcAI AGTACT 1 cut(s) 720
BmgT120I GGNCC 1 cut(s) 792
BmiI GGNNCC 2 cut(s) 120, 872
BmsI GCATC 2 cut(s) 379, 847
Bpu14I TTCGAA 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 373, 669
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 664, 815, 944
Bse1I ACTGG 3 cut(s) 511, 578, 753
Bse21I CCTNAGG 1 cut(s) 663
BseAI TCCGGA 1 cut(s) 58
BseDI CCNNGG 2 cut(s) 373, 669
BseGI GGATG 1 cut(s) 394
BseLI CCNNNNNNNGG 3 cut(s) 664, 815, 944
BseMII CTCAG 1 cut(s) 677
BseNI ACTGG 3 cut(s) 511, 578, 753
BseXI GCAGC 5 cut(s) 110, 161, 341, 497, 921
BsgI GTGCAG 2 cut(s) 399, 860
BshFI GGCC 3 cut(s) 43, 668, 793
BsiHKAI GWGCWC 1 cut(s) 704
BsiSI CCGG 2 cut(s) 44, 59
BslI CCNNNNNNNGG 3 cut(s) 664, 815, 944
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsnI GGCC 3 cut(s) 43, 668, 793
Bsp119I TTCGAA 1 cut(s) 298
Bsp1286I GDGCHC 2 cut(s) 704, 873
Bsp13I TCCGGA 1 cut(s) 58
Bsp143I GATC 6 cut(s) 118, 505, 796, 850, 880, 920
BspACI CCGC 2 cut(s) 188, 907
BspANI GGCC 3 cut(s) 43, 668, 793
BspCNI CTCAG 1 cut(s) 676
BspEI TCCGGA 1 cut(s) 58
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 120, 872
BspPI GGATC 4 cut(s) 113, 126, 845, 875
BspQI GCTCTTC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 298
BsrI ACTGG 3 cut(s) 511, 578, 753
BssECI CCNNGG 2 cut(s) 373, 669
BssMI GATC 6 cut(s) 118, 505, 796, 850, 880, 920
BssNI GRCGYC 1 cut(s) 453
BssSI CACGAG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 267
Bst4CI ACNGT 4 cut(s) 132, 622, 718, 785
Bst6I CTCTTC 3 cut(s) 129, 151, 480
BstACI GRCGYC 1 cut(s) 453
BstBI TTCGAA 1 cut(s) 298
BstC8I GCNNGC 4 cut(s) 139, 192, 483, 914
BstDEI CTNAG 1 cut(s) 663
BstDSI CCRYGG 1 cut(s) 669
BstF5I GGATG 1 cut(s) 394
BstKTI GATC 6 cut(s) 121, 508, 799, 853, 883, 923
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 6 cut(s) 118, 505, 796, 850, 880, 920
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNSI RCATGY 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 781
BstV1I GCAGC 5 cut(s) 110, 161, 341, 497, 921
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 3 cut(s) 43, 668, 793
BtgI CCRYGG 1 cut(s) 669
BtgZI GCGATG 2 cut(s) 503, 582
BtsCI GGATG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 790
Cac8I GCNNGC 4 cut(s) 139, 192, 483, 914
CaiI CAGNNNCTG 1 cut(s) 152
CciI TCATGA 1 cut(s) 735
Cfr13I GGNCC 1 cut(s) 792
CseI GACGC 1 cut(s) 244
Csp6I GTAC 5 cut(s) 49, 217, 646, 719, 926
CviAII CATG 6 cut(s) 95, 173, 233, 736, 827, 954
CviQI GTAC 5 cut(s) 49, 217, 646, 719, 926
DdeI CTNAG 1 cut(s) 663
DpnI GATC 6 cut(s) 120, 507, 798, 852, 882, 922
DpnII GATC 6 cut(s) 118, 505, 796, 850, 880, 920
EaeI YGGCCR 1 cut(s) 41
Eam1104I CTCTTC 3 cut(s) 129, 151, 480
EarI CTCTTC 3 cut(s) 129, 151, 480
Eco130I CCWWGG 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 873
Eco81I CCTNAGG 1 cut(s) 663
EcoRI GAATTC 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 1 cut(s) 373
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 6 cut(s) 98, 176, 236, 739, 830, 957
FalI AAGNNNNNCTT 2 cut(s) 459, 491
FatI CATG 6 cut(s) 94, 172, 232, 735, 826, 953
FauI CCCGC 1 cut(s) 195
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 5 cut(s) 99, 150, 330, 486, 910
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 5 cut(s) 99, 150, 330, 486, 910
FspBI CTAG 1 cut(s) 918
GluI GCNGC 5 cut(s) 99, 150, 330, 486, 910
HaeIII GGCC 3 cut(s) 43, 668, 793
HapII CCGG 2 cut(s) 44, 59
HgaI GACGC 1 cut(s) 244
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 6 cut(s) 98, 176, 236, 739, 830, 957
HincII GTYRAC 2 cut(s) 181, 259
HindII GTYRAC 2 cut(s) 181, 259
HinfI GANTC 3 cut(s) 286, 314, 494
HpaII CCGG 2 cut(s) 44, 59
HphI GGTGA 4 cut(s) 295, 500, 860, 981
Hpy166II GTNNAC 3 cut(s) 181, 259, 989
Hpy188I TCNGA 2 cut(s) 28, 563
Hpy188III TCNNGA 7 cut(s) 11, 59, 449, 597, 632, 736, 854
Hpy8I GTNNAC 3 cut(s) 181, 259, 989
HpyAV CCTTC 4 cut(s) 352, 412, 629, 815
HpyCH4III ACNGT 4 cut(s) 132, 622, 718, 785
HpyCH4IV ACGT 2 cut(s) 215, 453
HpyCH4V TGCA 8 cut(s) 98, 206, 293, 380, 392, 460, 481, 877
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 663
HpySE526I ACGT 2 cut(s) 215, 453
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 6 cut(s) 98, 176, 236, 739, 830, 957
Kpn2I TCCGGA 1 cut(s) 58
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 6 cut(s) 118, 505, 796, 850, 880, 920
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 2 cut(s) 770, 876
Lsp1109I GCAGC 5 cut(s) 110, 161, 341, 497, 921
LweI GCATC 2 cut(s) 379, 847
MaeI CTAG 1 cut(s) 918
MaeII ACGT 2 cut(s) 215, 453
MalI GATC 6 cut(s) 120, 507, 798, 852, 882, 922
MboI GATC 6 cut(s) 118, 505, 796, 850, 880, 920
MboII GAAGA 5 cut(s) 116, 168, 250, 467, 625
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 2 cut(s) 704, 873
MluCI AATT 2 cut(s) 29, 843
MmeI TCCRAC 1 cut(s) 792
MnlI CCTC 4 cut(s) 132, 544, 672, 970
MroI TCCGGA 1 cut(s) 58
MroXI GAANNNNTTC 2 cut(s) 81, 585
MseI TTAA 2 cut(s) 831, 887
MspA1I CMGCKG 2 cut(s) 152, 190
MspI CCGG 2 cut(s) 44, 59
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 6 cut(s) 118, 505, 796, 850, 880, 920
NlaIII CATG 6 cut(s) 98, 176, 236, 739, 830, 957
NlaIV GGNNCC 2 cut(s) 120, 872
NmeAIII GCCGAG 1 cut(s) 795
NspI RCATGY 1 cut(s) 176
NspV TTCGAA 1 cut(s) 298
PagI TCATGA 1 cut(s) 735
PciSI GCTCTTC 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 81, 585
PfeI GAWTC 3 cut(s) 286, 314, 494
PflFI GACNNNGTC 1 cut(s) 254
PflMI CCANNNNNTGG 1 cut(s) 944
PkrI GCNGC 5 cut(s) 100, 151, 331, 487, 911
PspN4I GGNNCC 2 cut(s) 120, 872
PspPI GGNCC 1 cut(s) 792
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 118
PsyI GACNNNGTC 1 cut(s) 254
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 5 cut(s) 50, 218, 647, 720, 927
RsaNI GTAC 5 cut(s) 49, 217, 646, 719, 926
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 831, 887
SatI GCNGC 5 cut(s) 99, 150, 330, 486, 910
Sau3AI GATC 6 cut(s) 118, 505, 796, 850, 880, 920
Sau96I GGNCC 1 cut(s) 792
ScaI AGTACT 1 cut(s) 720
SduI GDGCHC 2 cut(s) 704, 873
SetI ASST 9 cut(s) 154, 218, 222, 456, 472, 640, 654, 767, 914
SfaNI GCATC 2 cut(s) 379, 847
SfcI CTRYAG 1 cut(s) 781
SfuI TTCGAA 1 cut(s) 298
Sse9I AATT 2 cut(s) 29, 843
SsiI CCGC 2 cut(s) 188, 907
SspI AATATT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 918
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 4 cut(s) 132, 622, 718, 785
TaiI ACGT 2 cut(s) 218, 456
TaqI TCGA 3 cut(s) 85, 298, 345
TasI AATT 2 cut(s) 29, 843
TatI WGTACW 4 cut(s) 48, 645, 718, 925
TfiI GAWTC 3 cut(s) 286, 314, 494
Tru1I TTAA 2 cut(s) 831, 887
Tru9I TTAA 2 cut(s) 831, 887
TscAI CASTG 1 cut(s) 790
TseI GCWGC 5 cut(s) 98, 149, 329, 485, 909
TspDTI ATGAA 3 cut(s) 724, 752, 764
TspGWI ACGGA 1 cut(s) 686
TspRI CASTG 1 cut(s) 790
Tth111I GACNNNGTC 1 cut(s) 254
Van91I CCANNNNNTGG 1 cut(s) 944
XapI RAATTY 1 cut(s) 29
XceI RCATGY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XmnI GAANNNNTTC 2 cut(s) 81, 585
XspI CTAG 1 cut(s) 918
ZraI GACGTC 1 cut(s) 454
ZrmI AGTACT 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.