Rmu_sc0003801.1_g000024

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003801.1
OG0019818
Physical Location & Seq
Forward (+)
81826 .. 82404
579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
atgcttcttatgaaactcacattagtcttattagcagctgcaacgacattgctagctgcagcagctggtaaacccctcccaccgcctcaagccaagcccaactgcgcagaccgctgtggtgacctcacaattccatatccatttgggatcggtgacggctgttacatactactgaagggacagcaaaagcaattcgaactcacttgtgatgattccactaaacccccaacactgcactggaagcactacgaagacctcagtgtcactgattttaatgttactgagggtgagttggaatctatgaattttataagcaaggattcttacgacaagagcggctctaatgtggagaacagtcctttgctccgttggggtcctcctttcagcatttccggtcccaaaaacaggttcttcgccgtaggctgtgacacttctgctgttttccggggttttcgtggcgaagaagagttcatgacaggttgcatgtcagcttgtccgtgtgccccaatatcagcagcgtggatcaaaactcttgcactggtgtcgggttttgccagaccaaaatccctgaaggactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

192

Amino Acids

20.83

Weight (kDa)

6.13

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 98 1.3e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019818)

Species Orthologous Gene IDs
pyrus_communis pycom05g25280
rosa_chinensis RchiOBHm_Chr5g0016941
rosa_laevigata RLG00000032284
rosa_multiflora Rmu_sc0003630.1_g000063 Rmu_sc0003801.1_g000024 Rmu_sc0007023.1_g000008
rosa_rugosa Rorug05G0587100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AasI GACNNNNNNGTC 1 cut(s) 260
Acc16I TGCGCA 1 cut(s) 106
AccBSI CCGCTC 1 cut(s) 336
AciI CCGC 3 cut(s) 83, 112, 336
AclWI GGATC 2 cut(s) 155, 530
AcsI RAATTY 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 4 cut(s) 38, 56, 65, 491
AluI AGCT 4 cut(s) 38, 56, 65, 491
AlwI GGATC 2 cut(s) 155, 530
AlwNI CAGNNNCTG 1 cut(s) 65
ApeKI GCWGC 6 cut(s) 35, 38, 56, 59, 62, 515
ApoI RAATTY 1 cut(s) 304
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 2 cut(s) 374, 395
AsuC2I CCSGG 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 131, 164, 299
AsuII TTCGAA 1 cut(s) 195
AsuNHI GCTAGC 1 cut(s) 52
AvaII GGWCC 2 cut(s) 374, 395
BaeGI GKGCMC 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 6 cut(s) 25, 43, 47, 71, 74, 527
BceAI ACGGC 2 cut(s) 172, 401
BcgI CGANNNNNNTGC 2 cut(s) 525, 559
BcnI CCSGG 1 cut(s) 446
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 7 cut(s) 36, 39, 57, 60, 63, 337, 516
BlsI GCNGC 7 cut(s) 37, 40, 58, 61, 64, 338, 517
Bme1390I CCNGG 1 cut(s) 446
Bme18I GGWCC 2 cut(s) 374, 395
BmgT120I GGNCC 2 cut(s) 374, 395
BmiI GGNNCC 2 cut(s) 375, 397
BmrFI CCNGG 1 cut(s) 446
BmtI GCTAGC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 258
Bpu14I TTCGAA 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 392
Bsc4I CCNNNNNNNGG 1 cut(s) 405
Bse1I ACTGG 2 cut(s) 242, 543
Bse3DI GCAATG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 405
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 2 cut(s) 271, 273
BseNI ACTGG 2 cut(s) 242, 543
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 6 cut(s) 25, 43, 47, 71, 74, 527
BsgI GTGCAG 1 cut(s) 218
BsiSI CCGG 2 cut(s) 393, 445
BslFI GGGAC 2 cut(s) 192, 381
BslI CCNNNNNNNGG 1 cut(s) 405
BsmFI GGGAC 2 cut(s) 192, 381
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 2 cut(s) 147, 522
BspACI CCGC 3 cut(s) 83, 112, 336
BspCNI CTCAG 2 cut(s) 270, 274
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 2 cut(s) 375, 397
BspMAI CTGCAG 1 cut(s) 61
BspOI GCTAGC 1 cut(s) 56
BspPI GGATC 2 cut(s) 155, 530
BspT104I TTCGAA 1 cut(s) 195
BsrBI CCGCTC 1 cut(s) 336
BsrDI GCAATG 1 cut(s) 47
BsrI ACTGG 2 cut(s) 242, 543
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 2 cut(s) 147, 522
Bst4CI ACNGT 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 459
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 2 cut(s) 257, 282
BstEII GGTNACC 1 cut(s) 119
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 2 cut(s) 150, 525
BstMBI GATC 2 cut(s) 147, 522
BstMWI GCNNNNNNNGC 3 cut(s) 62, 111, 241
BstNSI RCATGY 1 cut(s) 487
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 444
BstSFI CTRYAG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 505
BstV1I GCAGC 6 cut(s) 25, 43, 47, 71, 74, 527
BstV2I GAAGAC 1 cut(s) 258
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 5 cut(s) 230, 235, 264, 265, 536
Cac8I GCNNGC 1 cut(s) 54
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 1 cut(s) 471
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 374, 395
CviAII CATG 2 cut(s) 472, 484
CviJI RGCY 9 cut(s) 38, 56, 65, 92, 97, 159, 339, 423, 491
CviKI_1 RGCY 9 cut(s) 38, 56, 65, 92, 97, 159, 339, 423, 491
DdeI CTNAG 2 cut(s) 257, 282
DpnI GATC 2 cut(s) 149, 524
DpnII GATC 2 cut(s) 147, 522
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 374, 395
Eco57I CTGAAG 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 374
EcoO65I GGTNACC 1 cut(s) 119
FaeI CATG 2 cut(s) 475, 487
FaiI YATR 7 cut(s) 11, 136, 167, 302, 311, 473, 485
FaqI GGGAC 2 cut(s) 192, 381
FatI CATG 2 cut(s) 471, 483
Fnu4HI GCNGC 7 cut(s) 36, 39, 57, 60, 63, 337, 516
Fsp4HI GCNGC 7 cut(s) 36, 39, 57, 60, 63, 337, 516
FspBI CTAG 1 cut(s) 53
FspI TGCGCA 1 cut(s) 106
GlaI GCGC 1 cut(s) 106
GluI GCNGC 7 cut(s) 36, 39, 57, 60, 63, 337, 516
HapII CCGG 2 cut(s) 393, 445
HhaI GCGC 1 cut(s) 107
Hin1II CATG 2 cut(s) 475, 487
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 3 cut(s) 212, 296, 320
HpaII CCGG 2 cut(s) 393, 445
HphI GGTGA 3 cut(s) 131, 164, 299
Hpy166II GTNNAC 1 cut(s) 71
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 169, 565
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4V TGCA 5 cut(s) 41, 59, 235, 483, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 111, 241
HpyF3I CTNAG 2 cut(s) 257, 282
Hsp92II CATG 2 cut(s) 475, 487
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 2 cut(s) 147, 522
LmnI GCTCC 1 cut(s) 369
LpnPI CCDG 8 cut(s) 51, 223, 391, 406, 458, 462, 524, 568
Lsp1109I GCAGC 6 cut(s) 25, 43, 47, 71, 74, 527
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 6 cut(s) 119, 152, 161, 262, 277, 425
MalI GATC 2 cut(s) 149, 524
MbiI CCGCTC 1 cut(s) 336
MboI GATC 2 cut(s) 147, 522
MboII GAAGA 4 cut(s) 263, 403, 473, 476
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 3 cut(s) 129, 191, 304
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 6 cut(s) 86, 96, 134, 266, 277, 387
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 3 cut(s) 38, 65, 114
MspI CCGG 2 cut(s) 393, 445
MspR9I CCNGG 1 cut(s) 446
MwoI GCNNNNNNNGC 3 cut(s) 62, 111, 241
NciI CCSGG 1 cut(s) 446
NdeII GATC 2 cut(s) 147, 522
NheI GCTAGC 1 cut(s) 52
NlaIII CATG 2 cut(s) 475, 487
NlaIV GGNNCC 2 cut(s) 375, 397
NmuCI GTSAC 4 cut(s) 119, 152, 262, 425
NsbI TGCGCA 1 cut(s) 106
NspI RCATGY 1 cut(s) 487
NspV TTCGAA 1 cut(s) 195
PagI TCATGA 1 cut(s) 471
PfeI GAWTC 3 cut(s) 212, 296, 320
PkrI GCNGC 7 cut(s) 37, 40, 58, 61, 64, 338, 517
PpuMI RGGWCCY 1 cut(s) 374
PsiI TTATAA 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 374
PspEI GGTNACC 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 375, 397
PspPI GGNCC 2 cut(s) 374, 395
PspPPI RGGWCCY 1 cut(s) 374
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 65
PvuII CAGCTG 2 cut(s) 38, 65
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 7 cut(s) 36, 39, 57, 60, 63, 337, 516
Sau3AI GATC 2 cut(s) 147, 522
Sau96I GGNCC 2 cut(s) 374, 395
ScrFI CCNGG 1 cut(s) 446
SduI GDGCHC 1 cut(s) 505
SetI ASST 8 cut(s) 40, 58, 67, 126, 258, 410, 481, 493
SfcI CTRYAG 1 cut(s) 57
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 2 cut(s) 374, 395
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
Sse9I AATT 3 cut(s) 129, 191, 304
SsiI CCGC 3 cut(s) 83, 112, 336
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 444
TaaI ACNGT 1 cut(s) 356
TaqI TCGA 1 cut(s) 195
TasI AATT 3 cut(s) 129, 191, 304
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 3 cut(s) 212, 296, 320
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 5 cut(s) 237, 242, 265, 271, 543
TseFI GTSAC 4 cut(s) 119, 152, 262, 425
TseI GCWGC 6 cut(s) 35, 38, 56, 59, 62, 515
Tsp45I GTSAC 4 cut(s) 119, 152, 262, 425
TspDTI ATGAA 3 cut(s) 26, 317, 460
TspGWI ACGGA 2 cut(s) 356, 486
TspRI CASTG 5 cut(s) 237, 242, 265, 271, 543
VpaK11BI GGWCC 2 cut(s) 374, 395
XapI RAATTY 1 cut(s) 304
XceI RCATGY 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 234
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.