Rmu_sc0003819.1_g000020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003819.1
OG0018773
Physical Location & Seq
Reverse (-)
83278 .. 83916
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003819.1_g000020.1.cds

Sequence Viewer

Length: 639 bp
atggaaagttggagaagacaagaaaatcaaggtgatgaagccacttacgaatcacagaagaacagaaagccattgagtgtgcctttctgggaacagaaattctgttggacggttgctggcaatgttccgtgggaatatttcttagagacgaagaagaatatgcatcgttacaaagacgttatgcaatggaacgactctgcagctgaagaggcaatcaattgtgcaaagaagcggtttctggataagattgacggccttccctgcaacataccactgcctgatcctgatgcttacattcataaaatagattggagcagctccagaactagtactggtccggaactggttacggataaccaagaaactcgaggcaaagatgaaggagaggttgttattgatgatcttgagtccctttcggatcagtcatcttggggcactggatggttgccgggggcatgggacgagtcggaggaggaatccgaagaagatgctaacagcagagtagataatgaagagaatcttgatctcaaggaagcagcagatgtagtaggatttgacaacagtattgggaatgaccgggataaaaacaaaatggtacaagaattgatgatggggttaattttggagagtccattgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

24.34

Weight (kDa)

4.35

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018773)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g08251
rosa_laevigata RLG00000016477
rosa_multiflora Rmu_sc0003819.1_g000020
rosa_roxburghii Rroxscaffold_2G00147260
rosa_rugosa Rorug02G0043200
rosa_samantha Rh2AG090800 Rh2BG090700 Rh2CG093200 Rh2DG089700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 1 cut(s) 232
AclWI GGATC 2 cut(s) 275, 426
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 2 cut(s) 331, 597
AhlI ACTAGT 1 cut(s) 326
AluBI AGCT 2 cut(s) 203, 318
AluI AGCT 2 cut(s) 203, 318
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 2 cut(s) 275, 426
Ama87I CYCGRG 1 cut(s) 366
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 3 cut(s) 200, 315, 536
ApoI RAATTY 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 2 cut(s) 450, 578
AsuHPI GGTGA 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 366
AvaII GGWCC 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 22
BbvI GCAGC 3 cut(s) 212, 327, 548
BccI CCATC 2 cut(s) 435, 604
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BcnI CCSGG 2 cut(s) 450, 578
BcoDI GTCTC 1 cut(s) 140
BcuI ACTAGT 1 cut(s) 326
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 3 cut(s) 201, 316, 537
BlsI GCNGC 3 cut(s) 202, 317, 538
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 2 cut(s) 450, 578
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 1 cut(s) 335
BmrFI CCNGG 2 cut(s) 450, 578
BmsI GCATC 3 cut(s) 172, 277, 478
BpiI GAAGAC 1 cut(s) 22
BpmI CTGGAG 1 cut(s) 304
BpuEI CTTGAG 2 cut(s) 425, 512
BpuMI CCSGG 2 cut(s) 450, 578
BsaJI CCNNGG 2 cut(s) 128, 449
BsaWI WCCGGW 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bse1I ACTGG 3 cut(s) 337, 348, 442
Bse3DI GCAATG 2 cut(s) 127, 191
BseAI TCCGGA 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 128, 449
BseGI GGATG 1 cut(s) 446
BseMI GCAATG 2 cut(s) 127, 191
BseNI ACTGG 3 cut(s) 337, 348, 442
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 437
BseXI GCAGC 3 cut(s) 212, 327, 548
BshFI GGCC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 3 cut(s) 338, 449, 577
BslFI GGGAC 2 cut(s) 394, 473
BsmAI GTCTC 1 cut(s) 140
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 2 cut(s) 394, 473
BsnI GGCC 1 cut(s) 255
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 1 cut(s) 437
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 4 cut(s) 280, 400, 418, 523
BspACI CCGC 1 cut(s) 232
BspANI GGCC 1 cut(s) 255
BspEI TCCGGA 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 2 cut(s) 275, 426
BsrDI GCAATG 2 cut(s) 127, 191
BsrI ACTGG 3 cut(s) 337, 348, 442
BssECI CCNNGG 2 cut(s) 128, 449
BssMI GATC 4 cut(s) 280, 400, 418, 523
Bst4CI ACNGT 2 cut(s) 112, 563
Bst6I CTCTTC 2 cut(s) 201, 507
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 142
BstDSI CCRYGG 1 cut(s) 128
BstF5I GGATG 1 cut(s) 446
BstKTI GATC 4 cut(s) 283, 403, 421, 526
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 4 cut(s) 280, 400, 418, 523
BstMWI GCNNNNNNNGC 2 cut(s) 209, 261
BstSCI CCNGG 2 cut(s) 448, 576
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 437
BstV1I GCAGC 3 cut(s) 212, 327, 548
BstV2I GAAGAC 1 cut(s) 22
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 128
BtsCI GGATG 1 cut(s) 446
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 2 cut(s) 272, 435
Cac8I GCNNGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 330, 596
CviAII CATG 1 cut(s) 456
CviJI RGCY 5 cut(s) 41, 70, 203, 255, 318
CviKI_1 RGCY 5 cut(s) 41, 70, 203, 255, 318
CviQI GTAC 2 cut(s) 330, 596
DdeI CTNAG 1 cut(s) 142
DpnI GATC 4 cut(s) 282, 402, 420, 525
DpnII GATC 4 cut(s) 280, 400, 418, 523
Eam1104I CTCTTC 2 cut(s) 201, 507
EarI CTCTTC 2 cut(s) 201, 507
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 1 cut(s) 459
FaiI YATR 5 cut(s) 161, 182, 269, 300, 457
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FaqI GGGAC 2 cut(s) 394, 473
FatI CATG 1 cut(s) 455
Fnu4HI GCNGC 3 cut(s) 201, 316, 537
FokI GGATG 1 cut(s) 453
Fsp4HI GCNGC 3 cut(s) 201, 316, 537
FspBI CTAG 1 cut(s) 327
GluI GCNGC 3 cut(s) 201, 316, 537
GsuI CTGGAG 1 cut(s) 304
HaeIII GGCC 1 cut(s) 255
HapII CCGG 3 cut(s) 338, 449, 577
Hin1II CATG 1 cut(s) 459
HinfI GANTC 7 cut(s) 50, 194, 407, 464, 476, 517, 628
HpaII CCGG 3 cut(s) 338, 449, 577
HphI GGTGA 1 cut(s) 44
Hpy188I TCNGA 3 cut(s) 418, 469, 481
Hpy188III TCNNGA 6 cut(s) 239, 284, 321, 338, 404, 521
HpyAV CCTTC 2 cut(s) 266, 374
HpyCH4III ACNGT 2 cut(s) 112, 563
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 163, 184, 200, 224, 264
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 261
HpyF3I CTNAG 1 cut(s) 142
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 1 cut(s) 459
Kpn2I TCCGGA 1 cut(s) 337
Kzo9I GATC 4 cut(s) 280, 400, 418, 523
LmnI GCTCC 2 cut(s) 312, 323
Lsp1109I GCAGC 3 cut(s) 212, 327, 548
LweI GCATC 3 cut(s) 172, 277, 478
MaeI CTAG 1 cut(s) 327
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 2 cut(s) 167, 346
MalI GATC 4 cut(s) 282, 402, 420, 525
MboI GATC 4 cut(s) 280, 400, 418, 523
MboII GAAGA 8 cut(s) 27, 70, 163, 166, 218, 494, 497, 524
MfeI CAATTG 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 4 cut(s) 98, 217, 602, 618
MlyI GAGTC 4 cut(s) 188, 416, 473, 637
MmeI TCCRAC 2 cut(s) 86, 447
MnlI CCTC 5 cut(s) 202, 362, 379, 463, 466
Mph1103I ATGCAT 1 cut(s) 165
MroI TCCGGA 1 cut(s) 337
MseI TTAA 1 cut(s) 617
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 3 cut(s) 338, 449, 577
MspR9I CCNGG 2 cut(s) 450, 578
MunI CAATTG 1 cut(s) 217
MwoI GCNNNNNNNGC 2 cut(s) 209, 261
NciI CCSGG 2 cut(s) 450, 578
NdeII GATC 4 cut(s) 280, 400, 418, 523
NlaIII CATG 1 cut(s) 459
NsiI ATGCAT 1 cut(s) 165
PaeR7I CTCGAG 1 cut(s) 366
PfeI GAWTC 3 cut(s) 50, 476, 517
PkrI GCNGC 3 cut(s) 202, 317, 538
PleI GAGTC 4 cut(s) 188, 415, 472, 636
PpsI GAGTC 4 cut(s) 188, 415, 472, 636
PspPI GGNCC 1 cut(s) 335
PspXI VCTCGAGB 1 cut(s) 366
PstI CTGCAG 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 2 cut(s) 331, 597
RsaNI GTAC 2 cut(s) 330, 596
SaqAI TTAA 1 cut(s) 617
SatI GCNGC 3 cut(s) 201, 316, 537
Sau3AI GATC 4 cut(s) 280, 400, 418, 523
Sau96I GGNCC 1 cut(s) 335
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 4 cut(s) 188, 416, 473, 637
ScrFI CCNGG 2 cut(s) 450, 578
SduI GDGCHC 1 cut(s) 437
SetI ASST 5 cut(s) 34, 180, 205, 320, 390
SfaNI GCATC 3 cut(s) 172, 277, 478
SfcI CTRYAG 1 cut(s) 198
Sfr274I CTCGAG 1 cut(s) 366
SinI GGWCC 1 cut(s) 335
SlaI CTCGAG 1 cut(s) 366
SmlI CTYRAG 3 cut(s) 366, 404, 527
SmoI CTYRAG 3 cut(s) 366, 404, 527
SpeI ACTAGT 1 cut(s) 326
Sse9I AATT 4 cut(s) 98, 217, 602, 618
SsiI CCGC 1 cut(s) 232
SspI AATATT 1 cut(s) 137
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 448, 576
TaaI ACNGT 2 cut(s) 112, 563
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 367
TasI AATT 4 cut(s) 98, 217, 602, 618
TatI WGTACW 1 cut(s) 329
TfiI GAWTC 3 cut(s) 50, 476, 517
Tru1I TTAA 1 cut(s) 617
Tru9I TTAA 1 cut(s) 617
TscAI CASTG 2 cut(s) 279, 442
TseI GCWGC 3 cut(s) 200, 315, 536
TspDTI ATGAA 4 cut(s) 51, 287, 393, 525
TspGWI ACGGA 2 cut(s) 117, 365
TspRI CASTG 2 cut(s) 279, 442
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 366
XspI CTAG 1 cut(s) 327
ZrmI AGTACT 1 cut(s) 331
Zsp2I ATGCAT 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.