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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 139 |
| AccI | GTMKAC | 1 cut(s) | 472 |
| AclI | AACGTT | 1 cut(s) | 780 |
| AclWI | GGATC | 3 cut(s) | 181, 643, 656 |
| AcsI | RAATTY | 3 cut(s) | 124, 236, 315 |
| AcuI | CTGAAG | 1 cut(s) | 502 |
| AfaI | GTAC | 1 cut(s) | 287 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 446 |
| AgeI | ACCGGT | 1 cut(s) | 439 |
| AgsI | TTSAA | 7 cut(s) | 87, 131, 193, 220, 307, 314, 617 |
| AjnI | CCWGG | 1 cut(s) | 49 |
| AluBI | AGCT | 4 cut(s) | 97, 576, 613, 745 |
| AluI | AGCT | 4 cut(s) | 97, 576, 613, 745 |
| Alw26I | GTCTC | 2 cut(s) | 155, 618 |
| AlwI | GGATC | 3 cut(s) | 181, 643, 656 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 416 |
| ApeKI | GCWGC | 1 cut(s) | 15 |
| ApoI | RAATTY | 3 cut(s) | 124, 236, 315 |
| AsiGI | ACCGGT | 1 cut(s) | 439 |
| AspA2I | CCTAGG | 1 cut(s) | 431 |
| AspS9I | GGNCC | 1 cut(s) | 554 |
| AsuHPI | GGTGA | 2 cut(s) | 652, 784 |
| AvaII | GGWCC | 1 cut(s) | 554 |
| AvrII | CCTAGG | 1 cut(s) | 431 |
| BamHI | GGATCC | 1 cut(s) | 648 |
| BanI | GGYRCC | 1 cut(s) | 139 |
| BanII | GRGCYC | 1 cut(s) | 327 |
| BbsI | GAAGAC | 1 cut(s) | 761 |
| BbvI | GCAGC | 1 cut(s) | 27 |
| BccI | CCATC | 2 cut(s) | 239, 660 |
| BciT130I | CCWGG | 1 cut(s) | 51 |
| BclI | TGATCA | 1 cut(s) | 379 |
| BcoDI | GTCTC | 2 cut(s) | 155, 618 |
| BfaI | CTAG | 4 cut(s) | 263, 354, 432, 669 |
| BisI | GCNGC | 1 cut(s) | 16 |
| BlnI | CCTAGG | 1 cut(s) | 431 |
| BlsI | GCNGC | 1 cut(s) | 17 |
| Bme1390I | CCNGG | 1 cut(s) | 51 |
| Bme18I | GGWCC | 1 cut(s) | 554 |
| BmgT120I | GGNCC | 1 cut(s) | 554 |
| BmiI | GGNNCC | 4 cut(s) | 141, 438, 555, 650 |
| BmrFI | CCNGG | 1 cut(s) | 51 |
| BpiI | GAAGAC | 1 cut(s) | 761 |
| BpuEI | CTTGAG | 1 cut(s) | 179 |
| BsaJI | CCNNGG | 3 cut(s) | 431, 549, 569 |
| BsaWI | WCCGGW | 1 cut(s) | 439 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 615, 645 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 446 |
| Bse118I | RCCGGY | 1 cut(s) | 439 |
| Bse1I | ACTGG | 1 cut(s) | 650 |
| Bse3DI | GCAATG | 1 cut(s) | 557 |
| BseBI | CCWGG | 1 cut(s) | 51 |
| BseDI | CCNNGG | 3 cut(s) | 431, 549, 569 |
| BseGI | GGATG | 2 cut(s) | 37, 652 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 446 |
| BseMI | GCAATG | 1 cut(s) | 557 |
| BseMII | CTCAG | 1 cut(s) | 90 |
| BseNI | ACTGG | 1 cut(s) | 650 |
| BseXI | GCAGC | 1 cut(s) | 27 |
| BshNI | GGYRCC | 1 cut(s) | 139 |
| BshTI | ACCGGT | 1 cut(s) | 439 |
| BsiSI | CCGG | 1 cut(s) | 440 |
| BslFI | GGGAC | 1 cut(s) | 567 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 446 |
| BsmAI | GTCTC | 2 cut(s) | 155, 618 |
| BsmFI | GGGAC | 1 cut(s) | 567 |
| Bsp1286I | GDGCHC | 1 cut(s) | 327 |
| Bsp143I | GATC | 3 cut(s) | 173, 379, 648 |
| BspCNI | CTCAG | 1 cut(s) | 89 |
| BspHI | TCATGA | 2 cut(s) | 382, 579 |
| BspLI | GGNNCC | 4 cut(s) | 141, 438, 555, 650 |
| BspPI | GGATC | 3 cut(s) | 181, 643, 656 |
| BspT107I | GGYRCC | 1 cut(s) | 139 |
| BsrDI | GCAATG | 1 cut(s) | 557 |
| BsrFI | RCCGGY | 1 cut(s) | 439 |
| BsrI | ACTGG | 1 cut(s) | 650 |
| BssAI | RCCGGY | 1 cut(s) | 439 |
| BssECI | CCNNGG | 3 cut(s) | 431, 549, 569 |
| BssMI | GATC | 3 cut(s) | 173, 379, 648 |
| BssT1I | CCWWGG | 3 cut(s) | 431, 549, 569 |
| Bst2UI | CCWGG | 1 cut(s) | 51 |
| Bst4CI | ACNGT | 2 cut(s) | 493, 500 |
| Bst6I | CTCTTC | 1 cut(s) | 19 |
| BstC8I | GCNNGC | 1 cut(s) | 689 |
| BstDEI | CTNAG | 1 cut(s) | 76 |
| BstF5I | GGATG | 2 cut(s) | 37, 652 |
| BstKTI | GATC | 3 cut(s) | 176, 382, 651 |
| BstMAI | GTCTC | 2 cut(s) | 155, 618 |
| BstMBI | GATC | 3 cut(s) | 173, 379, 648 |
| BstNI | CCWGG | 1 cut(s) | 51 |
| BstNSI | RCATGY | 1 cut(s) | 299 |
| BstSCI | CCNGG | 1 cut(s) | 49 |
| BstV1I | GCAGC | 1 cut(s) | 27 |
| BstV2I | GAAGAC | 1 cut(s) | 761 |
| BstX2I | RGATCY | 2 cut(s) | 173, 648 |
| BstYI | RGATCY | 2 cut(s) | 173, 648 |
| BtsCI | GGATG | 2 cut(s) | 37, 652 |
| BtsI | GCAGTG | 1 cut(s) | 331 |
| BtsIMutI | CAGTG | 4 cut(s) | 155, 331, 526, 685 |
| Cac8I | GCNNGC | 1 cut(s) | 689 |
| CaiI | CAGNNNCTG | 1 cut(s) | 416 |
| CciI | TCATGA | 2 cut(s) | 382, 579 |
| Cfr10I | RCCGGY | 1 cut(s) | 439 |
| Cfr13I | GGNCC | 1 cut(s) | 554 |
| Csp6I | GTAC | 1 cut(s) | 286 |
| CspAI | ACCGGT | 1 cut(s) | 439 |
| CviAII | CATG | 5 cut(s) | 296, 383, 542, 580, 628 |
| CviQI | GTAC | 1 cut(s) | 286 |
| DdeI | CTNAG | 1 cut(s) | 76 |
| DpnI | GATC | 3 cut(s) | 175, 381, 650 |
| DpnII | GATC | 3 cut(s) | 173, 379, 648 |
| DraI | TTTAAA | 1 cut(s) | 258 |
| Eam1104I | CTCTTC | 1 cut(s) | 19 |
| EarI | CTCTTC | 1 cut(s) | 19 |
| Eco130I | CCWWGG | 3 cut(s) | 431, 549, 569 |
| Eco24I | GRGCYC | 1 cut(s) | 327 |
| Eco32I | GATATC | 1 cut(s) | 750 |
| Eco47I | GGWCC | 1 cut(s) | 554 |
| Eco57I | CTGAAG | 1 cut(s) | 502 |
| EcoRII | CCWGG | 1 cut(s) | 49 |
| EcoRV | GATATC | 1 cut(s) | 750 |
| EcoT14I | CCWWGG | 3 cut(s) | 431, 549, 569 |
| EcoT38I | GRGCYC | 1 cut(s) | 327 |
| ErhI | CCWWGG | 3 cut(s) | 431, 549, 569 |
| FaeI | CATG | 5 cut(s) | 299, 386, 545, 583, 631 |
| FaiI | YATR | 9 cut(s) | 273, 275, 297, 321, 384, 543, 581, 629, 708 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 597, 629 |
| FaqI | GGGAC | 1 cut(s) | 567 |
| FatI | CATG | 5 cut(s) | 295, 382, 541, 579, 627 |
| FbaI | TGATCA | 1 cut(s) | 379 |
| FblI | GTMKAC | 1 cut(s) | 472 |
| Fnu4HI | GCNGC | 1 cut(s) | 16 |
| FokI | GGATG | 2 cut(s) | 44, 639 |
| FriOI | GRGCYC | 1 cut(s) | 327 |
| Fsp4HI | GCNGC | 1 cut(s) | 16 |
| FspBI | CTAG | 4 cut(s) | 263, 354, 432, 669 |
| GluI | GCNGC | 1 cut(s) | 16 |
| HapII | CCGG | 1 cut(s) | 440 |
| Hin1II | CATG | 5 cut(s) | 299, 386, 545, 583, 631 |
| HindIII | AAGCTT | 1 cut(s) | 95 |
| HinfI | GANTC | 4 cut(s) | 401, 407, 413, 538 |
| HpaII | CCGG | 1 cut(s) | 440 |
| HphI | GGTGA | 2 cut(s) | 652, 784 |
| Hpy166II | GTNNAC | 4 cut(s) | 400, 473, 496, 643 |
| Hpy188I | TCNGA | 4 cut(s) | 43, 406, 412, 418 |
| Hpy188III | TCNNGA | 7 cut(s) | 171, 374, 383, 504, 526, 580, 700 |
| Hpy8I | GTNNAC | 4 cut(s) | 400, 473, 496, 643 |
| HpyAV | CCTTC | 1 cut(s) | 29 |
| HpyCH4III | ACNGT | 2 cut(s) | 493, 500 |
| HpyCH4IV | ACGT | 2 cut(s) | 694, 780 |
| HpyCH4V | TGCA | 6 cut(s) | 15, 153, 283, 299, 336, 718 |
| HpyF3I | CTNAG | 1 cut(s) | 76 |
| HpySE526I | ACGT | 2 cut(s) | 694, 780 |
| Hsp92II | CATG | 5 cut(s) | 299, 386, 545, 583, 631 |
| Ksp22I | TGATCA | 1 cut(s) | 379 |
| Kzo9I | GATC | 3 cut(s) | 173, 379, 648 |
| LmnI | GCTCC | 1 cut(s) | 573 |
| Lsp1109I | GCAGC | 1 cut(s) | 27 |
| MaeI | CTAG | 4 cut(s) | 263, 354, 432, 669 |
| MaeII | ACGT | 2 cut(s) | 694, 780 |
| MaeIII | GTNAC | 3 cut(s) | 362, 544, 734 |
| MalI | GATC | 3 cut(s) | 175, 381, 650 |
| MboI | GATC | 3 cut(s) | 173, 379, 648 |
| MboII | GAAGA | 5 cut(s) | 33, 36, 205, 208, 766 |
| MflI | RGATCY | 2 cut(s) | 173, 648 |
| MhlI | GDGCHC | 1 cut(s) | 327 |
| MluCI | AATT | 7 cut(s) | 124, 236, 250, 315, 420, 455, 481 |
| MlyI | GAGTC | 2 cut(s) | 395, 532 |
| MmeI | TCCRAC | 2 cut(s) | 209, 601 |
| MseI | TTAA | 2 cut(s) | 99, 257 |
| MspI | CCGG | 1 cut(s) | 440 |
| MspR9I | CCNGG | 1 cut(s) | 51 |
| MvaI | CCWGG | 1 cut(s) | 51 |
| NdeII | GATC | 3 cut(s) | 173, 379, 648 |
| NlaIII | CATG | 5 cut(s) | 299, 386, 545, 583, 631 |
| NlaIV | GGNNCC | 4 cut(s) | 141, 438, 555, 650 |
| NmuCI | GTSAC | 3 cut(s) | 362, 544, 734 |
| NspI | RCATGY | 1 cut(s) | 299 |
| PagI | TCATGA | 2 cut(s) | 382, 579 |
| PfeI | GAWTC | 2 cut(s) | 407, 413 |
| PflFI | GACNNNGTC | 1 cut(s) | 629 |
| PinAI | ACCGGT | 1 cut(s) | 439 |
| PkrI | GCNGC | 1 cut(s) | 17 |
| PleI | GAGTC | 2 cut(s) | 395, 532 |
| PpsI | GAGTC | 2 cut(s) | 395, 532 |
| Psp1406I | AACGTT | 1 cut(s) | 780 |
| Psp6I | CCWGG | 1 cut(s) | 49 |
| PspGI | CCWGG | 1 cut(s) | 49 |
| PspN4I | GGNNCC | 4 cut(s) | 141, 438, 555, 650 |
| PspPI | GGNCC | 1 cut(s) | 554 |
| PstNI | CAGNNNCTG | 1 cut(s) | 416 |
| PsuI | RGATCY | 2 cut(s) | 173, 648 |
| PsyI | GACNNNGTC | 1 cut(s) | 629 |
| RsaI | GTAC | 1 cut(s) | 287 |
| RsaNI | GTAC | 1 cut(s) | 286 |
| SaqAI | TTAA | 2 cut(s) | 99, 257 |
| SatI | GCNGC | 1 cut(s) | 16 |
| Sau3AI | GATC | 3 cut(s) | 173, 379, 648 |
| Sau96I | GGNCC | 1 cut(s) | 554 |
| SchI | GAGTC | 2 cut(s) | 395, 532 |
| ScrFI | CCNGG | 1 cut(s) | 51 |
| SduI | GDGCHC | 1 cut(s) | 327 |
| SinI | GGWCC | 1 cut(s) | 554 |
| SmlI | CTYRAG | 1 cut(s) | 158 |
| SmoI | CTYRAG | 1 cut(s) | 158 |
| Sse9I | AATT | 7 cut(s) | 124, 236, 250, 315, 420, 455, 481 |
| SspMI | CTAG | 4 cut(s) | 263, 354, 432, 669 |
| StyD4I | CCNGG | 1 cut(s) | 49 |
| StyI | CCWWGG | 3 cut(s) | 431, 549, 569 |
| TaaI | ACNGT | 2 cut(s) | 493, 500 |
| TaiI | ACGT | 2 cut(s) | 697, 783 |
| TaqI | TCGA | 1 cut(s) | 752 |
| TasI | AATT | 7 cut(s) | 124, 236, 250, 315, 420, 455, 481 |
| TatI | WGTACW | 1 cut(s) | 285 |
| TfiI | GAWTC | 2 cut(s) | 407, 413 |
| Tru1I | TTAA | 2 cut(s) | 99, 257 |
| Tru9I | TTAA | 2 cut(s) | 99, 257 |
| TscAI | CASTG | 4 cut(s) | 155, 338, 526, 685 |
| TseFI | GTSAC | 3 cut(s) | 362, 544, 734 |
| TseI | GCWGC | 1 cut(s) | 15 |
| Tsp45I | GTSAC | 3 cut(s) | 362, 544, 734 |
| TspDTI | ATGAA | 3 cut(s) | 48, 568, 791 |
| TspGWI | ACGGA | 1 cut(s) | 726 |
| TspRI | CASTG | 4 cut(s) | 155, 338, 526, 685 |
| Tth111I | GACNNNGTC | 1 cut(s) | 629 |
| VpaK11BI | GGWCC | 1 cut(s) | 554 |
| XapI | RAATTY | 3 cut(s) | 124, 236, 315 |
| XceI | RCATGY | 1 cut(s) | 299 |
| XmaJI | CCTAGG | 1 cut(s) | 431 |
| XmiI | GTMKAC | 1 cut(s) | 472 |
| XspI | CTAG | 4 cut(s) | 263, 354, 432, 669 |