Rmu_sc0004999.1_g000015

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004999.1
OG0000030
Physical Location & Seq
Reverse (-)
63657 .. 69417
5761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004999.1_g000015.1.cds

Sequence Viewer

Length: 2583 bp
atgaagcaacctcctgggttgggcgcattctgtggtgagtttgagaaggtggccatggtgattgtgctaccaaatcttaatctagtggtgttttgtcatctgctatggtggcagctagcgggatttagacgctcggcaagagtctgttgtgggaccgcactgaaccagtttgtgagtgtaaaatatgagataataattgaagctcaaattggtggtctgaattgtaggcatgaaatattggcgaacactgttgaagaagcgaaagcaatggtttcgacgactgaggagggaggcacggagctaataagtagtgacagcggcatgcaaattgacgggactcaagtagcggctgttgcaaataactcgttgcttacatataaagcatcatcgtcggacacgatggagacagtttcctcccttgaagactccattggacatgagaatggtggtgaaaaattgggtaagtgtgatgggaaaactggagattgtccggtgagtcctatagtaggtcgacgcatgagggaatttagagcaatatgtactggtttgtcagaggagaagcaagtagctattgatgcaagtggttttggggctatattcacgtttaggtgtagcgaacttaacgtgcttctatgccacatgatgtgcaacaactttgatgttgaaaacacaacaatcaagattcacgggagacatttaacagtcacccctgcagacttccgccatgtactgagtataagggatggtggggctgacgtagatttaacaggaagcttatctgaaccggacattgtagagttgaagtccaagttttgtggtgttgatgaggaaataagcttggatgctgtggggaagatggtggcccaatcagaaactgctgatgatacatttataataagtttttgtctctatgccctgtcaactatgattttgccgagtgaaagtgatgcagtggatcccaggcttcttctcgccgtaaaggatccgaaagcgattagcaaaaaaaactgggcttcctttgcatttagaaagcttgttgaaggacttggaaggtataagaatggggtgtcctcaaatgtgtatgggtgtatcgcatttctgcagctgttttatttggatacaatgctaatgaagttccttgttgttccgacatacacatctccggtactctgttggactaacaagcaggcaagaattatgtatgaccaggcagaggctaatggtgggtatgactcgactggtataatagcgaccaagcgttggtcgacaactcgattccttgataatgccggatctgttgacgcaagtacaggactgaaaacaactattgttgaagatctagacaaggtcaggaacgatgttgatgctttcaaatgcgaatttgaggaggttagatcttgcatatctcatatagaaccggacatggtgggcctccgcaatgctattatgacactgcaaacatctatttgtgaaatgagggatagagggatgagtaacatggttgtagaagtgatgaaggaagtccttgatgaggagggttcgtttgagcagaaggggagggatgaatcagatatgtacgaagatgacatgtgtcccagtcagcggggatcgccgagtgtcgttgggccgcataagtctaatatggataatgttaaggacaaaagcttgtcattgaagcgaaagtcagacaattgtgttgatgatgacaacatggtcaaattatgtgcagttggtgatatatccaacagcgagtcatttctgattacttacttgttcagcaaggtgatggctgagtctgacagtcttgcaagttactgtgtaatgctgacgtccaatattttagattgtagacatatcagttgcatacctgtttcaaaaatcaagcgacatatggttcatgcccacatatttctccgtaagttaaaagaggaacgtgctaaggactacacaagtggtagtgttgtagacgggttgatatcatctgtggtgactatatgtcgccttcgtagatatagcggccgattgggttcttgcaagcagattttcttccctctgcgcgacggtgttgtggatagctggtttttggtggttttgaatctggaggtgggggaattagagatttgggacagctgccccgacatgaatgccagacagcgtcgtgagtcttatggaaatgctgtggtggagctatttcagaatgtttttagtaatgaaataagattgtttcccgcactttatgcaagactagcttcagtgtctatagtatatcctaaagagggcccgaccaacccaaagactcaggattccggtatatttgtaatccggaacatgcagtattaccggaaacgctggtttgaagggttcaactccggtgaccaaaggatccgccttgctgttgatatagtgaatcacccaatgaatgcagtgcgtgtctctgtatgggcagcagcagctcaggaaactccaattgatgctaaatcatttggacgtgcagcatacaacgggaacttcatccctaatcgcaatattccacttgatatagcaggaatgaaattcaaggctcgtcggacttcgcggcattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

860

Amino Acids

95.53

Weight (kDa)

5.81

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000030)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09630 FvH4_1g25481 FvH4_2g06390 FvH4_2g09470 FvH4_2g11952 FvH4_2g25151 FvH4_2g27901 FvH4_3g22392 FvH4_3g23130 FvH4_3g26751 FvH4_3g37244 FvH4_3g37260 FvH4_4g03329 FvH4_4g03350 FvH4_4g07720 FvH4_4g07721 FvH4_4g09520 FvH4_4g14081 FvH4_4g14090 FvH4_4g16060 FvH4_4g16061 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_5g19752 FvH4_5g19752 FvH4_5g19752 FvH4_5g33480 FvH4_6g25711 FvH4_6g25720 FvH4_6g40321 FvH4_6g40680 FvH4_6g40680 FvH4_7g05770 FvH4_7g05770 FvH4_7g06960 FvH4_7g07131 FvH4_7g07131 FvH4_7g07131 FvH4_7g07131 FvH4_7g08580 FvH4_7g10221 FvH4_7g12251 FvH4_7g12251 FvH4_7g12251 FvH4_c19g00020
prunus_persica Prupe.3G127800_v2.0.a1 Prupe.3G127900_v2.0.a1 Prupe.3G127900_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0313581 RchiOBHm_Chr1g0347831 RchiOBHm_Chr1g0372251 RchiOBHm_Chr2g0120671 RchiOBHm_Chr2g0120681 RchiOBHm_Chr2g0155601 RchiOBHm_Chr2g0161371 RchiOBHm_Chr2g0171911 RchiOBHm_Chr3g0451001 RchiOBHm_Chr3g0478081 RchiOBHm_Chr3g0485951 RchiOBHm_Chr4g0396211 RchiOBHm_Chr4g0396221 RchiOBHm_Chr4g0413121 RchiOBHm_Chr4g0417291 RchiOBHm_Chr4g0423011 RchiOBHm_Chr4g0424781 RchiOBHm_Chr4g0427951 RchiOBHm_Chr4g0432021 RchiOBHm_Chr5g0012051 RchiOBHm_Chr6g0268131 RchiOBHm_Chr6g0268141 RchiOBHm_Chr6g0281911 RchiOBHm_Chr6g0285311 RchiOBHm_Chr7g0189591 RchiOBHm_Chr7g0189601 RchiOBHm_Chr7g0224131
rosa_laevigata RLG00000000963 RLG00000000967 RLG00000001615 RLG00000002043 RLG00000002094 RLG00000002112 RLG00000002404 RLG00000004455 RLG00000005527 RLG00000005655 RLG00000006174 RLG00000007767 RLG00000008355 RLG00000008585 RLG00000008588 RLG00000008683 RLG00000008813 RLG00000009112 RLG00000009252 RLG00000009280 RLG00000009413 RLG00000012815 RLG00000012845 RLG00000013323 RLG00000013324 RLG00000015631 RLG00000017303 RLG00000018309 RLG00000018310 RLG00000018442 RLG00000018443 RLG00000018702 RLG00000019612 RLG00000021285 RLG00000021286 RLG00000022541 RLG00000023201 RLG00000023427 RLG00000029113 RLG00000029669 RLG00000030439 RLG00000030440 RLG00000033139 RLG00000034155 RLG00000034156 RLG00000034157 RLG00000034189 RLG00000034333 RLG00000034334 RLG00000034402 RLG00000034483
rosa_multiflora Rmu_co8408521.1_g000001 Rmu_co8451191.1_g000001 Rmu_co8509671.1_g000001 Rmu_co8512787.1_g000002 Rmu_sc0000077.1_g000042 Rmu_sc0000279.1_g000028 Rmu_sc0000280.1_g000005 Rmu_sc0000280.1_g000013 Rmu_sc0000319.1_g000017 Rmu_sc0000319.1_g000018 Rmu_sc0000361.1_g000007 Rmu_sc0000381.1_g000022 Rmu_sc0000642.1_g000014 Rmu_sc0000931.1_g000013 Rmu_sc0000982.1_g000033 Rmu_sc0001554.1_g000014 Rmu_sc0001992.1_g000027 Rmu_sc0002045.1_g000030 Rmu_sc0002106.1_g000002 Rmu_sc0003179.1_g000034 Rmu_sc0003422.1_g000009 Rmu_sc0004125.1_g000004 Rmu_sc0004999.1_g000015 Rmu_sc0005394.1_g000005 Rmu_sc0005712.1_g000015 Rmu_sc0006373.1_g000010 Rmu_sc0006872.1_g000002 Rmu_sc0006872.1_g000004 Rmu_sc0007367.1_g000011 Rmu_sc0007367.1_g000012 Rmu_sc0007491.1_g000005 Rmu_sc0007705.1_g000008 Rmu_sc0007705.1_g000009 Rmu_sc0007877.1_g000016 Rmu_sc0012512.1_g000006 Rmu_sc0021854.1_g000001 Rmu_sc0028193.1_g000003 Rmu_sc0032675.1_g000001 Rmu_ssc0000158.1_g000019 Rmu_ssc0000387.1_g000020
rosa_roxburghii Rroxscaffold_1G00009730 Rroxscaffold_1G00017900 Rroxscaffold_1G00031860 Rroxscaffold_1G00036000 Rroxscaffold_1G00036390 Rroxscaffold_1G00069020 Rroxscaffold_1G00071750 Rroxscaffold_2G00080160 Rroxscaffold_2G00080340 Rroxscaffold_2G00080790 Rroxscaffold_2G00085870 Rroxscaffold_2G00109300 Rroxscaffold_2G00113740 Rroxscaffold_2G00113750 Rroxscaffold_2G00113760 Rroxscaffold_2G00116400 Rroxscaffold_2G00116870 Rroxscaffold_2G00116880 Rroxscaffold_2G00118190 Rroxscaffold_2G00118200 Rroxscaffold_2G00118650 Rroxscaffold_2G00129110 Rroxscaffold_2G00134740 Rroxscaffold_2G00140920 Rroxscaffold_4G00285970 Rroxscaffold_4G00312160 Rroxscaffold_4G00322340 Rroxscaffold_5G00349950 Rroxscaffold_6G00430610 Rroxscaffold_7G00162630 Rroxscaffold_7G00162640 Rroxscaffold_7G00164320 Rroxscaffold_7G00214930
rosa_rugosa Rorug01G0012500 Rorug01G0018000 Rorug01G0019000 Rorug01G0019000 Rorug01G0070900 Rorug01G0094400 Rorug02G0043200 Rorug03G0329700 Rorug03G0329800 Rorug03G0329900 Rorug03G0332900 Rorug03G0343500 Rorug03G0343600 Rorug03G0343700 Rorug04G0032000 Rorug04G0065800 Rorug05G0102700 Rorug05G0106200 Rorug05G0256700 Rorug05G0256700 Rorug05G0439000 Rorug05G0540900 Rorug06G0055100 Rorug06G0060400 Rorug06G0170800 Rorug06G0184400 Rorug06G0439700 Rorug07G0182300 Rorug07G0182400 Rorug07G0182500 Rorug07G0208100 Rorug07G0208200 Rorug07G0208200 Rorug07G0283700.1 Rorug07G0283800
rosa_samantha Rh1AG090000 Rh1AG169000 Rh1BG060800 Rh1BG060900 Rh1BG070000 Rh1BG407100 Rh1BG407200 Rh1BG407400 Rh1BG407500 Rh1CG028100 Rh1CG028200 Rh1CG040100 Rh1CG050100 Rh1CG055500 Rh1CG156800 Rh1CG421400 Rh1CG421500 Rh1CG421600 Rh1DG029300 Rh1DG054600 Rh1DG054700 Rh1DG059400 Rh1DG177400 Rh1DG438200 Rh1DG438300 Rh1DG438500 Rh1DG438600 Rh1DG438700 Rh2AG286700 Rh2AG286800 Rh2AG286900 Rh2AG311900 Rh2AG322600 Rh2AG353300 Rh2AG353400 Rh2AG560900 Rh2AG642800 Rh2AG642900 Rh2AG643000 Rh2AG643100 Rh2AG647000 Rh2BG288800 Rh2BG317900 Rh2BG394800 Rh2BG394900 Rh2BG594000 Rh2BG657700 Rh2CG299000 Rh2CG299100 Rh2CG300200 Rh2DG102500 Rh2DG284000 Rh2DG312200 Rh2DG312300 Rh2DG336300 Rh2DG336400 Rh2DG337200 Rh2DG571300 Rh3AG246800 Rh3AG272200 Rh3BG121800 Rh3BG249400 Rh3BG265300 Rh3BG341500 Rh3BG348700 Rh3CG301300 Rh3CG307800 Rh3CG307900 Rh3CG308100 Rh3DG144400 Rh3DG251600 Rh3DG297700 Rh3DG301700 Rh3DG305100 Rh3DG340900 Rh4AG149000 Rh4AG248900 Rh4AG336100 Rh4BG044000 Rh4BG111400 Rh4BG133400 Rh4BG182300 Rh4BG219600 Rh4BG219700 Rh4BG254300 Rh4BG388300 Rh4BG388600 Rh4CG126200 Rh4CG155800 Rh4CG257000 Rh4CG402900 Rh4CG403000 Rh4DG005400 Rh4DG057400 Rh4DG057500 Rh4DG057700 Rh4DG120700 Rh4DG280800 Rh4DG338600 Rh5AG057600 Rh5AG057700 Rh5AG057800 Rh5AG284700 Rh5AG284800 Rh5AG305600 Rh5AG364300 Rh5AG365100 Rh5AG365200 Rh5AG516000 Rh5AG516200 Rh5BG157600 Rh5BG235200 Rh5BG235400 Rh5BG308900 Rh5BG373800 Rh5BG414500 Rh5BG538800 Rh5CG102300 Rh5CG248400 Rh5CG249300 Rh5CG249400 Rh5CG326300 Rh5CG326400 Rh5CG366400 Rh5CG366500 Rh5CG439100 Rh5CG439200 Rh5CG497700 Rh5CG562500 Rh5CG567300 Rh5DG179600 Rh5DG179700 Rh5DG239900 Rh5DG299700 Rh5DG319400 Rh5DG319500 Rh6AG173400 Rh6AG175300 Rh6AG314800 Rh6AG405000 Rh6BG067300 Rh6BG067400 Rh6BG075300 Rh6BG101700 Rh6BG134300 Rh6BG324500 Rh6CG087200 Rh6CG132400 Rh6CG151800 Rh6CG151900 Rh6CG200100 Rh6CG205500 Rh6CG419400 Rh6CG419500 Rh6DG081400 Rh6DG119900 Rh6DG120000 Rh6DG191500 Rh6DG199900 Rh6DG251900 Rh6DG252000 Rh6DG498400 Rh7AG274800 Rh7AG283100 Rh7AG352600 Rh7AG364900 Rh7AG386800 Rh7BG321800 Rh7BG341100 Rh7CG294000 Rh7CG368900 Rh7CG369900 Rh7CG383700 Rh7CG406000 Rh7CG406100 Rh7DG106300 Rh7DG106400 Rh7DG286300 Rh7DG346900 Rh7DG353400 Rh7DG360000 Rh7DG361700 Rh7DG362200 Rh7DG362600 Rh7DG362900 Rh7DG363400
rosa_wichuraiana Rw0G000990 Rw0G001740 Rw0G002300 Rw0G002310 Rw0G007890 Rw0G007900 Rw0G023450 Rw0G023460 Rw1G000300 Rw1G007140 Rw1G007170 Rw1G008970 Rw1G010460 Rw1G039090 Rw1G039100 Rw2G012530 Rw2G016610 Rw2G023010 Rw2G031730 Rw3G014310 Rw4G008550 Rw4G010240 Rw4G012160 Rw4G024150 Rw5G009090 Rw5G047250 Rw5G048500 Rw5G050510 Rw6G006430 Rw6G006440 Rw6G006450 Rw6G013330 Rw6G016560 Rw6G021330 Rw6G024630 Rw6G035440 Rw6G041540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
AasI GACNNNNNNGTC 1 cut(s) 1712
AatII GACGTC 1 cut(s) 1832
AccB7I CCANNNNNTGG 1 cut(s) 1260
AccI GTMKAC 4 cut(s) 511, 1265, 1849, 1965
AccII CGCG 2 cut(s) 2058, 2575
AccIII TCCGGA 1 cut(s) 2322
AclWI GGATC 8 cut(s) 950, 963, 977, 990, 1300, 1615, 2377, 2390
AcoI YGGCCR 2 cut(s) 51, 2017
AcsI RAATTY 3 cut(s) 524, 1379, 2552
AcuI CTGAAG 1 cut(s) 2235
AcyI GRCGYC 1 cut(s) 1829
AfaI GTAC 5 cut(s) 541, 729, 1167, 1309, 1577
AfiI CCNNNNNNNGG 7 cut(s) 20, 506, 1213, 1260, 1531, 1602, 2276
AflIII ACRYGT 1 cut(s) 1587
AhdI GACNNNNNGTC 1 cut(s) 1995
AjiI CACGTC 1 cut(s) 2489
AjnI CCWGG 3 cut(s) 13, 959, 1206
AjuI GAANNNNNNNTTGG 4 cut(s) 192, 224, 414, 446
Alw26I GTCTC 4 cut(s) 398, 685, 911, 2437
AlwI GGATC 8 cut(s) 950, 963, 977, 990, 1300, 1615, 2377, 2390
AlwNI CAGNNNCTG 1 cut(s) 875
Aor13HI TCCGGA 1 cut(s) 2322
AoxI GGCC 6 cut(s) 51, 861, 1429, 1625, 2017, 2278
ApaI GGGCCC 1 cut(s) 2282
ApeKI GCWGC 7 cut(s) 112, 1102, 2130, 2444, 2447, 2450, 2492
ApoI RAATTY 3 cut(s) 524, 1379, 2552
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspLEI GCGC 2 cut(s) 26, 2058
AspS9I GGNCC 6 cut(s) 153, 862, 1429, 1625, 2278, 2279
AsuNHI GCTAGC 1 cut(s) 115
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 1 cut(s) 2282
BalI TGGCCA 1 cut(s) 53
BamHI GGATCC 3 cut(s) 955, 982, 2382
BanII GRGCYC 1 cut(s) 2282
BbsI GAAGAC 1 cut(s) 429
BbvI GCAGC 7 cut(s) 124, 1114, 2117, 2456, 2459, 2462, 2504
BccI CCATC 5 cut(s) 394, 464, 737, 850, 1780
BceAI ACGGC 1 cut(s) 959
BciT130I CCWGG 3 cut(s) 15, 961, 1208
BciVI GTATCC 1 cut(s) 1111
BcoDI GTCTC 4 cut(s) 398, 685, 911, 2437
BfaI CTAG 4 cut(s) 83, 116, 1340, 2246
BfmI CTRYAG 4 cut(s) 501, 711, 1100, 2259
BfuI GTATCC 1 cut(s) 1111
BglII AGATCT 2 cut(s) 1336, 1394
Bme1390I CCNGG 3 cut(s) 15, 961, 1208
Bme18I GGWCC 1 cut(s) 153
BmeRI GACNNNNNGTC 1 cut(s) 1995
BmgBI CACGTC 1 cut(s) 2489
BmgT120I GGNCC 6 cut(s) 153, 862, 1429, 1625, 2278, 2279
BmiI GGNNCC 5 cut(s) 154, 957, 984, 2280, 2384
BmrFI CCNGG 3 cut(s) 15, 961, 1208
BmrI ACTGGG 2 cut(s) 1018, 1590
BmsI GCATC 6 cut(s) 392, 565, 832, 937, 1354, 2461
BmtI GCTAGC 1 cut(s) 119
BmuI ACTGGG 2 cut(s) 1018, 1590
BoxI GACNNNNGTC 1 cut(s) 1590
BpiI GAAGAC 1 cut(s) 429
BpmI CTGGAG 2 cut(s) 501, 2120
Bpu10I CCTNAGC 2 cut(s) 1938, 2454
BpuEI CTTGAG 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 1829
BsaJI CCNNGG 3 cut(s) 14, 54, 959
BsaWI WCCGGW 8 cut(s) 490, 784, 1162, 1417, 2306, 2322, 2340, 2369
BsaXI ACNNNNNCTCC 2 cut(s) 1526, 1556
Bsc4I CCNNNNNNNGG 7 cut(s) 20, 506, 1213, 1260, 1531, 1602, 2276
Bse1I ACTGG 6 cut(s) 166, 484, 547, 1013, 1243, 1596
Bse3DI GCAATG 2 cut(s) 273, 1444
BseAI TCCGGA 1 cut(s) 2322
BseBI CCWGG 3 cut(s) 15, 961, 1208
BseDI CCNNGG 3 cut(s) 14, 54, 959
BseGI GGATG 5 cut(s) 748, 847, 1494, 1567, 2511
BseLI CCNNNNNNNGG 7 cut(s) 20, 506, 1213, 1260, 1531, 1602, 2276
BseMI GCAATG 2 cut(s) 273, 1444
BseMII CTCAG 5 cut(s) 273, 722, 1782, 2312, 2468
BseNI ACTGG 6 cut(s) 166, 484, 547, 1013, 1243, 1596
BseRI GAGGAG 4 cut(s) 299, 569, 1400, 1547
BseSI GKGCMC 1 cut(s) 2282
BseX3I CGGCCG 1 cut(s) 2017
BseXI GCAGC 7 cut(s) 124, 1114, 2117, 2456, 2459, 2462, 2504
BsgI GTGCAG 2 cut(s) 1746, 2511
Bsh1236I CGCG 2 cut(s) 2058, 2575
Bsh1285I CGRYCG 1 cut(s) 2020
BshFI GGCC 6 cut(s) 53, 863, 1431, 1627, 2019, 2280
BsiEI CGRYCG 1 cut(s) 2020
BsiSI CCGG 9 cut(s) 491, 785, 1163, 1290, 1418, 2307, 2323, 2341, 2370
BslFI GGGAC 4 cut(s) 166, 349, 1578, 2138
BslI CCNNNNNNNGG 7 cut(s) 20, 506, 1213, 1260, 1531, 1602, 2276
BsmAI GTCTC 4 cut(s) 398, 685, 911, 2437
BsmFI GGGAC 4 cut(s) 166, 349, 1578, 2138
BsmI GAATGC 3 cut(s) 26, 2149, 2425
BsnI GGCC 6 cut(s) 53, 863, 1431, 1627, 2019, 2280
Bsp120I GGGCCC 1 cut(s) 2278
Bsp1286I GDGCHC 1 cut(s) 2282
Bsp13I TCCGGA 1 cut(s) 2322
Bsp143I GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
Bsp19I CCATGG 1 cut(s) 54
BspANI GGCC 6 cut(s) 53, 863, 1431, 1627, 2019, 2280
BspCNI CTCAG 5 cut(s) 274, 723, 1783, 2311, 2467
BspEI TCCGGA 1 cut(s) 2322
BspFNI CGCG 2 cut(s) 2058, 2575
BspLI GGNNCC 5 cut(s) 154, 957, 984, 2280, 2384
BspMAI CTGCAG 2 cut(s) 715, 1104
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 8 cut(s) 950, 963, 977, 990, 1300, 1615, 2377, 2390
BsrDI GCAATG 2 cut(s) 273, 1444
BsrI ACTGG 6 cut(s) 166, 484, 547, 1013, 1243, 1596
BssECI CCNNGG 3 cut(s) 14, 54, 959
BssMI GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
BssNI GRCGYC 1 cut(s) 1829
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 3 cut(s) 15, 961, 1208
Bst4CI ACNGT 6 cut(s) 250, 409, 703, 1802, 1817, 2063
BstACI GRCGYC 1 cut(s) 1829
BstAPI GCANNNNNTGC 1 cut(s) 2237
BstC8I GCNNGC 4 cut(s) 117, 323, 1188, 2036
BstDEI CTNAG 6 cut(s) 282, 731, 1791, 1938, 2298, 2454
BstDSI CCRYGG 1 cut(s) 54
BstEII GGTNACC 1 cut(s) 2372
BstENI CCTNNNNNAGG 2 cut(s) 504, 1529
BstF5I GGATG 5 cut(s) 748, 847, 1494, 1567, 2511
BstFNI CGCG 2 cut(s) 2058, 2575
BstHHI GCGC 2 cut(s) 26, 2058
BstKTI GATC 7 cut(s) 958, 985, 1295, 1339, 1397, 1610, 2385
BstMAI GTCTC 4 cut(s) 398, 685, 911, 2437
BstMBI GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
BstMCI CGRYCG 1 cut(s) 2020
BstMWI GCNNNNNNNGC 8 cut(s) 109, 353, 575, 1019, 1609, 2237, 2246, 2450
BstNI CCWGG 3 cut(s) 15, 961, 1208
BstNSI RCATGY 3 cut(s) 325, 1591, 2332
BstPAI GACNNNNGTC 1 cut(s) 1590
BstPI GGTNACC 1 cut(s) 2372
BstSCI CCNGG 3 cut(s) 13, 959, 1206
BstSFI CTRYAG 4 cut(s) 501, 711, 1100, 2259
BstSLI GKGCMC 1 cut(s) 2282
BstUI CGCG 2 cut(s) 2058, 2575
BstV1I GCAGC 7 cut(s) 124, 1114, 2117, 2456, 2459, 2462, 2504
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 6 cut(s) 955, 982, 1292, 1336, 1394, 2382
BstYI RGATCY 6 cut(s) 955, 982, 1292, 1336, 1394, 2382
BstZI CGGCCG 1 cut(s) 2017
BsuI GTATCC 1 cut(s) 1111
BsuRI GGCC 6 cut(s) 53, 863, 1431, 1627, 2019, 2280
BtgI CCRYGG 1 cut(s) 54
BtrI CACGTC 1 cut(s) 2489
BtsCI GGATG 5 cut(s) 748, 847, 1494, 1567, 2511
BtsI GCAGTG 3 cut(s) 957, 1451, 2430
BtsIMutI CAGTG 6 cut(s) 158, 246, 957, 1451, 2259, 2430
Cac8I GCNNGC 4 cut(s) 117, 323, 1188, 2036
CaiI CAGNNNCTG 1 cut(s) 875
CfoI GCGC 2 cut(s) 26, 2058
Cfr13I GGNCC 6 cut(s) 153, 862, 1429, 1625, 2278, 2279
CseI GACGC 4 cut(s) 138, 522, 1310, 2144
Csp6I GTAC 5 cut(s) 540, 728, 1166, 1308, 1576
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviQI GTAC 5 cut(s) 540, 728, 1166, 1308, 1576
DdeI CTNAG 6 cut(s) 282, 731, 1791, 1938, 2298, 2454
DpnI GATC 7 cut(s) 957, 984, 1294, 1338, 1396, 1609, 2384
DpnII GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
DrdI GACNNNNNNGTC 1 cut(s) 1712
DriI GACNNNNNGTC 1 cut(s) 1995
DseDI GACNNNNNNGTC 1 cut(s) 1712
EaeI YGGCCR 2 cut(s) 51, 2017
EagI CGGCCG 1 cut(s) 2017
Eam1105I GACNNNNNGTC 1 cut(s) 1995
EciI GGCGGA 2 cut(s) 710, 2375
EclXI CGGCCG 1 cut(s) 2017
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 2282
Eco32I GATATC 1 cut(s) 1977
Eco47I GGWCC 1 cut(s) 153
Eco52I CGGCCG 1 cut(s) 2017
Eco57I CTGAAG 1 cut(s) 2235
Eco91I GGTNACC 1 cut(s) 2372
EcoNI CCTNNNNNAGG 2 cut(s) 504, 1529
EcoO109I RGGNCCY 1 cut(s) 2278
EcoO65I GGTNACC 1 cut(s) 2372
EcoRII CCWGG 3 cut(s) 13, 959, 1206
EcoRV GATATC 1 cut(s) 1977
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 2282
ErhI CCWWGG 1 cut(s) 54
FalI AAGNNNNNCTT 2 cut(s) 2233, 2265
FaqI GGGAC 4 cut(s) 166, 349, 1578, 2138
FauI CCCGC 3 cut(s) 112, 1596, 2236
FauNDI CATATG 1 cut(s) 1890
FblI GTMKAC 4 cut(s) 511, 1265, 1849, 1965
FokI GGATG 5 cut(s) 755, 854, 1501, 1574, 2498
FriOI GRGCYC 1 cut(s) 2282
FspBI CTAG 4 cut(s) 83, 116, 1340, 2246
GlaI GCGC 2 cut(s) 25, 2057
GsuI CTGGAG 2 cut(s) 501, 2120
HaeIII GGCC 6 cut(s) 53, 863, 1431, 1627, 2019, 2280
HapII CCGG 9 cut(s) 491, 785, 1163, 1290, 1418, 2307, 2323, 2341, 2370
HgaI GACGC 4 cut(s) 138, 522, 1310, 2144
HhaI GCGC 2 cut(s) 26, 2058
Hin1I GRCGYC 1 cut(s) 1829
Hin6I GCGC 2 cut(s) 24, 2056
HinP1I GCGC 2 cut(s) 24, 2056
HincII GTYRAC 4 cut(s) 512, 921, 1266, 1300
HindII GTYRAC 4 cut(s) 512, 921, 1266, 1300
HindIII AAGCTT 4 cut(s) 772, 835, 1031, 1663
HpaII CCGG 9 cut(s) 491, 785, 1163, 1290, 1418, 2307, 2323, 2341, 2370
Hpy166II GTNNAC 6 cut(s) 512, 921, 1266, 1300, 1850, 1966
Hpy188III TCNNGA 8 cut(s) 679, 1340, 1351, 2099, 2159, 2300, 2323, 2456
Hpy8I GTNNAC 6 cut(s) 512, 921, 1266, 1300, 1850, 1966
Hpy99I CGWCG 6 cut(s) 280, 394, 516, 2063, 2160, 2568
HpyAV CCTTC 7 cut(s) 40, 1034, 1044, 1510, 1546, 2012, 2351
HpyCH4III ACNGT 6 cut(s) 250, 409, 703, 1802, 1817, 2063
HpyCH4IV ACGT 6 cut(s) 602, 624, 756, 1829, 1933, 2488
HpyF10VI GCNNNNNNNGC 8 cut(s) 109, 353, 575, 1019, 1609, 2237, 2246, 2450
HpyF3I CTNAG 6 cut(s) 282, 731, 1791, 1938, 2298, 2454
HpySE526I ACGT 6 cut(s) 602, 624, 756, 1829, 1933, 2488
Hsp92I GRCGYC 1 cut(s) 1829
HspAI GCGC 2 cut(s) 24, 2056
Kpn2I TCCGGA 1 cut(s) 2322
Kzo9I GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
LmnI GCTCC 2 cut(s) 298, 2185
Lsp1109I GCAGC 7 cut(s) 124, 1114, 2117, 2456, 2459, 2462, 2504
LweI GCATC 6 cut(s) 392, 565, 832, 937, 1354, 2461
MaeI CTAG 4 cut(s) 83, 116, 1340, 2246
MaeII ACGT 6 cut(s) 602, 624, 756, 1829, 1933, 2488
MaeIII GTNAC 6 cut(s) 311, 703, 1493, 1811, 1987, 2372
MalI GATC 7 cut(s) 957, 984, 1294, 1338, 1396, 1609, 2384
MboI GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
MboII GAAGA 7 cut(s) 266, 434, 865, 959, 1346, 1592, 2038
MfeI CAATTG 2 cut(s) 1690, 2466
MflI RGATCY 6 cut(s) 955, 982, 1292, 1336, 1394, 2382
MhlI GDGCHC 1 cut(s) 2282
MlsI TGGCCA 1 cut(s) 53
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 9 cut(s) 150, 331, 419, 505, 1226, 1760, 1802, 2171, 2290
MmeI TCCRAC 5 cut(s) 372, 1154, 1172, 1767, 2546
Mox20I TGGCCA 1 cut(s) 53
MroI TCCGGA 1 cut(s) 2322
MscI TGGCCA 1 cut(s) 53
MseI TTAA 6 cut(s) 78, 621, 698, 764, 1653, 1922
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 4 cut(s) 318, 1105, 1603, 2130
MspI CCGG 9 cut(s) 491, 785, 1163, 1290, 1418, 2307, 2323, 2341, 2370
MspR9I CCNGG 3 cut(s) 15, 961, 1208
MunI CAATTG 2 cut(s) 1690, 2466
Mva1269I GAATGC 3 cut(s) 26, 2149, 2425
MvaI CCWGG 3 cut(s) 15, 961, 1208
MvnI CGCG 2 cut(s) 2058, 2575
MwoI GCNNNNNNNGC 8 cut(s) 109, 353, 575, 1019, 1609, 2237, 2246, 2450
NcoI CCATGG 1 cut(s) 54
NdeI CATATG 1 cut(s) 1890
NdeII GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
NheI GCTAGC 1 cut(s) 115
NlaIV GGNNCC 5 cut(s) 154, 957, 984, 2280, 2384
NmeAIII GCCGAG 3 cut(s) 113, 960, 1638
NmuCI GTSAC 4 cut(s) 311, 703, 1987, 2372
NspI RCATGY 3 cut(s) 325, 1591, 2332
PaeI GCATGC 1 cut(s) 325
PciI ACATGT 1 cut(s) 1587
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PctI GAATGC 3 cut(s) 26, 2149, 2425
PfeI GAWTC 6 cut(s) 682, 1275, 1565, 2095, 2303, 2407
PflFI GACNNNGTC 2 cut(s) 1346, 2154
PflMI CCANNNNNTGG 1 cut(s) 1260
PleI GAGTC 9 cut(s) 149, 331, 419, 504, 1226, 1759, 1801, 2170, 2290
PpsI GAGTC 9 cut(s) 149, 331, 419, 504, 1226, 1759, 1801, 2170, 2290
PscI ACATGT 1 cut(s) 1587
PshAI GACNNNNGTC 1 cut(s) 1590
PsiI TTATAA 1 cut(s) 893
Psp6I CCWGG 3 cut(s) 13, 959, 1206
PspEI GGTNACC 1 cut(s) 2372
PspGI CCWGG 3 cut(s) 13, 959, 1206
PspN4I GGNNCC 5 cut(s) 154, 957, 984, 2280, 2384
PspOMI GGGCCC 1 cut(s) 2278
PspPI GGNCC 6 cut(s) 153, 862, 1429, 1625, 2278, 2279
PstI CTGCAG 2 cut(s) 715, 1104
PstNI CAGNNNCTG 1 cut(s) 875
PsuI RGATCY 6 cut(s) 955, 982, 1292, 1336, 1394, 2382
PsyI GACNNNGTC 2 cut(s) 1346, 2154
PvuII CAGCTG 2 cut(s) 1105, 2130
RsaI GTAC 5 cut(s) 541, 729, 1167, 1309, 1577
RsaNI GTAC 5 cut(s) 540, 728, 1166, 1308, 1576
RseI CAYNNNNRTG 1 cut(s) 441
SalI GTCGAC 2 cut(s) 510, 1264
SaqAI TTAA 6 cut(s) 78, 621, 698, 764, 1653, 1922
Sau3AI GATC 7 cut(s) 955, 982, 1292, 1336, 1394, 1607, 2382
Sau96I GGNCC 6 cut(s) 153, 862, 1429, 1625, 2278, 2279
SchI GAGTC 9 cut(s) 150, 331, 419, 505, 1226, 1760, 1802, 2171, 2290
ScrFI CCNGG 3 cut(s) 15, 961, 1208
SduI GDGCHC 1 cut(s) 2282
SfaNI GCATC 6 cut(s) 392, 565, 832, 937, 1354, 2461
SfcI CTRYAG 4 cut(s) 501, 711, 1100, 2259
SinI GGWCC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 339
SmoI CTYRAG 1 cut(s) 339
SphI GCATGC 1 cut(s) 325
SspI AATATT 3 cut(s) 237, 1837, 2527
SspMI CTAG 4 cut(s) 83, 116, 1340, 2246
StyD4I CCNGG 3 cut(s) 13, 959, 1206
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 6 cut(s) 250, 409, 703, 1802, 1817, 2063
TaiI ACGT 6 cut(s) 605, 627, 759, 1832, 1936, 2491
TaqI TCGA 5 cut(s) 275, 511, 1235, 1265, 1273
TatI WGTACW 3 cut(s) 539, 727, 1307
TauI GCSGC 5 cut(s) 321, 350, 1630, 2019, 2578
TfiI GAWTC 6 cut(s) 682, 1275, 1565, 2095, 2303, 2407
Tru1I TTAA 6 cut(s) 78, 621, 698, 764, 1653, 1922
Tru9I TTAA 6 cut(s) 78, 621, 698, 764, 1653, 1922
TscAI CASTG 6 cut(s) 165, 253, 957, 1458, 2259, 2430
TseFI GTSAC 4 cut(s) 311, 703, 1987, 2372
TseI GCWGC 7 cut(s) 112, 1102, 2130, 2444, 2447, 2450, 2492
Tsp45I GTSAC 4 cut(s) 311, 703, 1987, 2372
TspGWI ACGGA 2 cut(s) 311, 1904
TspRI CASTG 6 cut(s) 165, 253, 957, 1458, 2259, 2430
Tth111I GACNNNGTC 2 cut(s) 1346, 2154
Van91I CCANNNNNTGG 1 cut(s) 1260
VpaK11BI GGWCC 1 cut(s) 153
XagI CCTNNNNNAGG 2 cut(s) 504, 1529
XapI RAATTY 3 cut(s) 524, 1379, 2552
XbaI TCTAGA 1 cut(s) 1339
XceI RCATGY 3 cut(s) 325, 1591, 2332
XmiI GTMKAC 4 cut(s) 511, 1265, 1849, 1965
XspI CTAG 4 cut(s) 83, 116, 1340, 2246
ZraI GACGTC 1 cut(s) 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.