No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 570, 936, 959 |
| AclWI | GGATC | 2 cut(s) | 658, 671 |
| AcoI | YGGCCR | 1 cut(s) | 616 |
| AcsI | RAATTY | 4 cut(s) | 276, 301, 470, 731 |
| AcuI | CTGAAG | 1 cut(s) | 141 |
| AfaI | GTAC | 2 cut(s) | 73, 219 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 188, 189, 482, 1048 |
| AgsI | TTSAA | 7 cut(s) | 98, 115, 215, 257, 745, 866, 898 |
| AjnI | CCWGG | 1 cut(s) | 554 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 171, 203, 228, 249, 260, 281 |
| AleI | CACNNNNGTG | 2 cut(s) | 87, 776 |
| AluBI | AGCT | 2 cut(s) | 128, 914 |
| AluI | AGCT | 2 cut(s) | 128, 914 |
| Alw26I | GTCTC | 3 cut(s) | 41, 193, 314 |
| AlwI | GGATC | 2 cut(s) | 658, 671 |
| Ama87I | CYCGRG | 1 cut(s) | 1095 |
| AoxI | GGCC | 4 cut(s) | 444, 616, 963, 984 |
| ApeKI | GCWGC | 4 cut(s) | 128, 914, 956, 1074 |
| ApoI | RAATTY | 4 cut(s) | 276, 301, 470, 731 |
| AseI | ATTAAT | 1 cut(s) | 209 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 27, 60 |
| AsuC2I | CCSGG | 1 cut(s) | 651 |
| AsuHPI | GGTGA | 5 cut(s) | 281, 415, 486, 916, 938 |
| AvaI | CYCGRG | 1 cut(s) | 1095 |
| BalI | TGGCCA | 1 cut(s) | 618 |
| BamHI | GGATCC | 1 cut(s) | 663 |
| BauI | CACGAG | 1 cut(s) | 1043 |
| BbvI | GCAGC | 4 cut(s) | 115, 901, 943, 1086 |
| BccI | CCATC | 4 cut(s) | 297, 475, 675, 1086 |
| BceAI | ACGGC | 1 cut(s) | 321 |
| BciT130I | CCWGG | 1 cut(s) | 556 |
| BcnI | CCSGG | 1 cut(s) | 651 |
| BcoDI | GTCTC | 3 cut(s) | 41, 193, 314 |
| BisI | GCNGC | 5 cut(s) | 129, 570, 915, 957, 1075 |
| BlpI | GCTNAGC | 1 cut(s) | 284 |
| BlsI | GCNGC | 5 cut(s) | 130, 571, 916, 958, 1076 |
| Bme1390I | CCNGG | 2 cut(s) | 556, 651 |
| BmeT110I | CYCGRG | 1 cut(s) | 1095 |
| BmiI | GGNNCC | 3 cut(s) | 180, 665, 1053 |
| BmrFI | CCNGG | 2 cut(s) | 556, 651 |
| BmsI | GCATC | 1 cut(s) | 834 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 284 |
| BpuEI | CTTGAG | 1 cut(s) | 776 |
| BpuMI | CCSGG | 1 cut(s) | 651 |
| BsaI | GGTCTC | 1 cut(s) | 41 |
| BsaJI | CCNNGG | 4 cut(s) | 555, 638, 774, 981 |
| BsaWI | WCCGGW | 1 cut(s) | 74 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 188, 189, 482, 1048 |
| Bse3DI | GCAATG | 2 cut(s) | 798, 892 |
| BseBI | CCWGG | 1 cut(s) | 556 |
| BseDI | CCNNGG | 4 cut(s) | 555, 638, 774, 981 |
| BseGI | GGATG | 4 cut(s) | 176, 289, 565, 667 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 188, 189, 482, 1048 |
| BseMI | GCAATG | 2 cut(s) | 798, 892 |
| BseMII | CTCAG | 4 cut(s) | 31, 298, 325, 819 |
| BseRI | GAGGAG | 1 cut(s) | 854 |
| BseXI | GCAGC | 4 cut(s) | 115, 901, 943, 1086 |
| BseYI | CCCAGC | 2 cut(s) | 166, 707 |
| BsgI | GTGCAG | 1 cut(s) | 837 |
| BshFI | GGCC | 4 cut(s) | 446, 618, 965, 986 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1095 |
| BsiSI | CCGG | 2 cut(s) | 75, 650 |
| BslFI | GGGAC | 2 cut(s) | 205, 610 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 188, 189, 482, 1048 |
| BsmAI | GTCTC | 3 cut(s) | 41, 193, 314 |
| BsmFI | GGGAC | 2 cut(s) | 205, 610 |
| BsnI | GGCC | 4 cut(s) | 446, 618, 965, 986 |
| Bso31I | GGTCTC | 1 cut(s) | 41 |
| BsoBI | CYCGRG | 1 cut(s) | 1095 |
| Bsp143I | GATC | 3 cut(s) | 158, 663, 848 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 284 |
| BspACI | CCGC | 3 cut(s) | 570, 936, 959 |
| BspANI | GGCC | 4 cut(s) | 446, 618, 965, 986 |
| BspCNI | CTCAG | 4 cut(s) | 30, 297, 324, 818 |
| BspLI | GGNNCC | 3 cut(s) | 180, 665, 1053 |
| BspPI | GGATC | 2 cut(s) | 658, 671 |
| BspTNI | GGTCTC | 1 cut(s) | 41 |
| BsrDI | GCAATG | 2 cut(s) | 798, 892 |
| BssECI | CCNNGG | 4 cut(s) | 555, 638, 774, 981 |
| BssMI | GATC | 3 cut(s) | 158, 663, 848 |
| BssSI | CACGAG | 1 cut(s) | 1043 |
| BssT1I | CCWWGG | 2 cut(s) | 638, 981 |
| Bst2BI | CACGAG | 1 cut(s) | 1043 |
| Bst2UI | CCWGG | 1 cut(s) | 556 |
| Bst4CI | ACNGT | 4 cut(s) | 624, 778, 993, 1019 |
| Bst6I | CTCTTC | 2 cut(s) | 435, 535 |
| BstDEI | CTNAG | 7 cut(s) | 17, 155, 226, 284, 311, 507, 805 |
| BstDSI | CCRYGG | 1 cut(s) | 774 |
| BstF5I | GGATG | 4 cut(s) | 176, 289, 565, 667 |
| BstKTI | GATC | 3 cut(s) | 161, 666, 851 |
| BstMAI | GTCTC | 3 cut(s) | 41, 193, 314 |
| BstMBI | GATC | 3 cut(s) | 158, 663, 848 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 962, 1007 |
| BstNI | CCWGG | 1 cut(s) | 556 |
| BstSCI | CCNGG | 2 cut(s) | 554, 649 |
| BstV1I | GCAGC | 4 cut(s) | 115, 901, 943, 1086 |
| BstX2I | RGATCY | 1 cut(s) | 663 |
| BstYI | RGATCY | 1 cut(s) | 663 |
| BsuRI | GGCC | 4 cut(s) | 446, 618, 965, 986 |
| BtgI | CCRYGG | 1 cut(s) | 774 |
| BtsCI | GGATG | 4 cut(s) | 176, 289, 565, 667 |
| BtsI | GCAGTG | 1 cut(s) | 579 |
| BtsIMutI | CAGTG | 1 cut(s) | 579 |
| Csp6I | GTAC | 2 cut(s) | 72, 218 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 763, 798 |
| CviAII | CATG | 4 cut(s) | 65, 433, 595, 919 |
| CviQI | GTAC | 2 cut(s) | 72, 218 |
| DdeI | CTNAG | 7 cut(s) | 17, 155, 226, 284, 311, 507, 805 |
| DpnI | GATC | 3 cut(s) | 160, 665, 850 |
| DpnII | GATC | 3 cut(s) | 158, 663, 848 |
| EaeI | YGGCCR | 1 cut(s) | 616 |
| Eam1104I | CTCTTC | 2 cut(s) | 435, 535 |
| EarI | CTCTTC | 2 cut(s) | 435, 535 |
| Eco130I | CCWWGG | 2 cut(s) | 638, 981 |
| Eco31I | GGTCTC | 1 cut(s) | 41 |
| Eco32I | GATATC | 2 cut(s) | 492, 603 |
| Eco57I | CTGAAG | 1 cut(s) | 141 |
| Eco88I | CYCGRG | 1 cut(s) | 1095 |
| EcoRI | GAATTC | 1 cut(s) | 731 |
| EcoRII | CCWGG | 1 cut(s) | 554 |
| EcoRV | GATATC | 2 cut(s) | 492, 603 |
| EcoT14I | CCWWGG | 2 cut(s) | 638, 981 |
| ErhI | CCWWGG | 2 cut(s) | 638, 981 |
| FaeI | CATG | 4 cut(s) | 68, 436, 598, 922 |
| FaqI | GGGAC | 2 cut(s) | 205, 610 |
| FatI | CATG | 4 cut(s) | 64, 432, 594, 918 |
| FauNDI | CATATG | 1 cut(s) | 387 |
| Fnu4HI | GCNGC | 5 cut(s) | 129, 570, 915, 957, 1075 |
| FokI | GGATG | 4 cut(s) | 183, 276, 572, 654 |
| Fsp4HI | GCNGC | 5 cut(s) | 129, 570, 915, 957, 1075 |
| GluI | GCNGC | 5 cut(s) | 129, 570, 915, 957, 1075 |
| GsaI | CCCAGC | 2 cut(s) | 170, 711 |
| HaeIII | GGCC | 4 cut(s) | 446, 618, 965, 986 |
| HapII | CCGG | 2 cut(s) | 75, 650 |
| Hin1II | CATG | 4 cut(s) | 68, 436, 598, 922 |
| HincII | GTYRAC | 1 cut(s) | 382 |
| HindII | GTYRAC | 1 cut(s) | 382 |
| HinfI | GANTC | 3 cut(s) | 56, 503, 1098 |
| HpaII | CCGG | 2 cut(s) | 75, 650 |
| HphI | GGTGA | 5 cut(s) | 281, 415, 486, 916, 938 |
| Hpy166II | GTNNAC | 2 cut(s) | 236, 382 |
| Hpy188I | TCNGA | 6 cut(s) | 20, 121, 142, 808, 1065, 1103 |
| Hpy188III | TCNNGA | 1 cut(s) | 1095 |
| Hpy8I | GTNNAC | 2 cut(s) | 236, 382 |
| HpyAV | CCTTC | 5 cut(s) | 92, 251, 923, 935, 1042 |
| HpyCH4III | ACNGT | 4 cut(s) | 624, 778, 993, 1019 |
| HpyCH4V | TGCA | 7 cut(s) | 371, 791, 836, 854, 860, 1010, 1074 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 962, 1007 |
| HpyF3I | CTNAG | 7 cut(s) | 17, 155, 226, 284, 311, 507, 805 |
| Hsp92II | CATG | 4 cut(s) | 68, 436, 598, 922 |
| Kzo9I | GATC | 3 cut(s) | 158, 663, 848 |
| Lsp1109I | GCAGC | 4 cut(s) | 115, 901, 943, 1086 |
| LweI | GCATC | 1 cut(s) | 834 |
| MaeIII | GTNAC | 2 cut(s) | 403, 1019 |
| MalI | GATC | 3 cut(s) | 160, 665, 850 |
| MboI | GATC | 3 cut(s) | 158, 663, 848 |
| MboII | GAAGA | 6 cut(s) | 16, 21, 404, 452, 552, 626 |
| MflI | RGATCY | 1 cut(s) | 663 |
| MlsI | TGGCCA | 1 cut(s) | 618 |
| MluNI | TGGCCA | 1 cut(s) | 618 |
| MmeI | TCCRAC | 1 cut(s) | 1043 |
| Mox20I | TGGCCA | 1 cut(s) | 618 |
| MroXI | GAANNNNTTC | 2 cut(s) | 27, 60 |
| MscI | TGGCCA | 1 cut(s) | 618 |
| MseI | TTAA | 2 cut(s) | 209, 515 |
| MslI | CAYNNNNRTG | 3 cut(s) | 87, 89, 776 |
| Msp20I | TGGCCA | 1 cut(s) | 618 |
| MspI | CCGG | 2 cut(s) | 75, 650 |
| MspR9I | CCNGG | 2 cut(s) | 556, 651 |
| MvaI | CCWGG | 1 cut(s) | 556 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 962, 1007 |
| NciI | CCSGG | 1 cut(s) | 651 |
| NdeI | CATATG | 1 cut(s) | 387 |
| NdeII | GATC | 3 cut(s) | 158, 663, 848 |
| NlaIII | CATG | 4 cut(s) | 68, 436, 598, 922 |
| NlaIV | GGNNCC | 3 cut(s) | 180, 665, 1053 |
| NmuCI | GTSAC | 2 cut(s) | 403, 1019 |
| OliI | CACNNNNGTG | 2 cut(s) | 87, 776 |
| PdmI | GAANNNNTTC | 2 cut(s) | 27, 60 |
| PfeI | GAWTC | 2 cut(s) | 56, 503 |
| PflFI | GACNNNGTC | 1 cut(s) | 799 |
| PkrI | GCNGC | 5 cut(s) | 130, 571, 916, 958, 1076 |
| PshBI | ATTAAT | 1 cut(s) | 209 |
| Psp6I | CCWGG | 1 cut(s) | 554 |
| PspFI | CCCAGC | 2 cut(s) | 166, 707 |
| PspGI | CCWGG | 1 cut(s) | 554 |
| PspN4I | GGNNCC | 3 cut(s) | 180, 665, 1053 |
| PsuI | RGATCY | 1 cut(s) | 663 |
| PsyI | GACNNNGTC | 1 cut(s) | 799 |
| RsaI | GTAC | 2 cut(s) | 73, 219 |
| RsaNI | GTAC | 2 cut(s) | 72, 218 |
| RseI | CAYNNNNRTG | 3 cut(s) | 87, 89, 776 |
| SaqAI | TTAA | 2 cut(s) | 209, 515 |
| SatI | GCNGC | 5 cut(s) | 129, 570, 915, 957, 1075 |
| Sau3AI | GATC | 3 cut(s) | 158, 663, 848 |
| ScrFI | CCNGG | 2 cut(s) | 556, 651 |
| SfaNI | GCATC | 1 cut(s) | 834 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 87, 89, 776 |
| SmlI | CTYRAG | 1 cut(s) | 755 |
| SmoI | CTYRAG | 1 cut(s) | 755 |
| SsiI | CCGC | 3 cut(s) | 570, 936, 959 |
| SspI | AATATT | 1 cut(s) | 187 |
| StyD4I | CCNGG | 2 cut(s) | 554, 649 |
| StyI | CCWWGG | 2 cut(s) | 638, 981 |
| TaaI | ACNGT | 4 cut(s) | 624, 778, 993, 1019 |
| TaqI | TCGA | 1 cut(s) | 881 |
| TatI | WGTACW | 1 cut(s) | 217 |
| TauI | GCSGC | 1 cut(s) | 572 |
| TfiI | GAWTC | 2 cut(s) | 56, 503 |
| Tru1I | TTAA | 2 cut(s) | 209, 515 |
| Tru9I | TTAA | 2 cut(s) | 209, 515 |
| TscAI | CASTG | 1 cut(s) | 579 |
| TseFI | GTSAC | 2 cut(s) | 403, 1019 |
| TseI | GCWGC | 4 cut(s) | 128, 914, 956, 1074 |
| Tsp45I | GTSAC | 2 cut(s) | 403, 1019 |
| TspDTI | ATGAA | 6 cut(s) | 17, 53, 69, 576, 903, 935 |
| TspGWI | ACGGA | 2 cut(s) | 465, 1046 |
| TspRI | CASTG | 1 cut(s) | 579 |
| Tth111I | GACNNNGTC | 1 cut(s) | 799 |
| VspI | ATTAAT | 1 cut(s) | 209 |
| XapI | RAATTY | 4 cut(s) | 276, 301, 470, 731 |
| XmnI | GAANNNNTTC | 2 cut(s) | 27, 60 |