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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 66 |
| AgsI | TTSAA | 1 cut(s) | 134 |
| ApoI | RAATTY | 1 cut(s) | 66 |
| AspLEI | GCGC | 1 cut(s) | 112 |
| BciVI | GTATCC | 1 cut(s) | 90 |
| BfuI | GTATCC | 1 cut(s) | 90 |
| BpuEI | CTTGAG | 1 cut(s) | 122 |
| Bsp143I | GATC | 2 cut(s) | 46, 123 |
| BssMI | GATC | 2 cut(s) | 46, 123 |
| BstHHI | GCGC | 1 cut(s) | 112 |
| BstKTI | GATC | 2 cut(s) | 49, 126 |
| BstMBI | GATC | 2 cut(s) | 46, 123 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 116 |
| BsuI | GTATCC | 1 cut(s) | 90 |
| CfoI | GCGC | 1 cut(s) | 112 |
| DpnI | GATC | 2 cut(s) | 48, 125 |
| DpnII | GATC | 2 cut(s) | 46, 123 |
| FaiI | YATR | 1 cut(s) | 89 |
| GlaI | GCGC | 1 cut(s) | 111 |
| HhaI | GCGC | 1 cut(s) | 112 |
| Hin6I | GCGC | 1 cut(s) | 110 |
| HinP1I | GCGC | 1 cut(s) | 110 |
| HpyCH4V | TGCA | 1 cut(s) | 145 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 116 |
| HspAI | GCGC | 1 cut(s) | 110 |
| Kzo9I | GATC | 2 cut(s) | 46, 123 |
| LpnPI | CCDG | 1 cut(s) | 112 |
| MalI | GATC | 2 cut(s) | 48, 125 |
| MboI | GATC | 2 cut(s) | 46, 123 |
| MfeI | CAATTG | 1 cut(s) | 15 |
| MluCI | AATT | 2 cut(s) | 15, 66 |
| MseI | TTAA | 1 cut(s) | 9 |
| MunI | CAATTG | 1 cut(s) | 15 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 116 |
| NdeII | GATC | 2 cut(s) | 46, 123 |
| SaqAI | TTAA | 1 cut(s) | 9 |
| Sau3AI | GATC | 2 cut(s) | 46, 123 |
| SetI | ASST | 1 cut(s) | 152 |
| SgeI | CNNG | 7 cut(s) | 50, 68, 83, 119, 139, 151, 163 |
| SmlI | CTYRAG | 1 cut(s) | 137 |
| SmoI | CTYRAG | 1 cut(s) | 137 |
| Sse9I | AATT | 2 cut(s) | 15, 66 |
| TaqI | TCGA | 1 cut(s) | 42 |
| TasI | AATT | 2 cut(s) | 15, 66 |
| Tru1I | TTAA | 1 cut(s) | 9 |
| Tru9I | TTAA | 1 cut(s) | 9 |
| XapI | RAATTY | 1 cut(s) | 66 |