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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 30 |
| BbsI | GAAGAC | 2 cut(s) | 81, 134 |
| BmsI | GCATC | 1 cut(s) | 36 |
| BpiI | GAAGAC | 2 cut(s) | 81, 134 |
| BpmI | CTGGAG | 1 cut(s) | 103 |
| BsgI | GTGCAG | 1 cut(s) | 81 |
| BstV2I | GAAGAC | 2 cut(s) | 81, 134 |
| Eco57I | CTGAAG | 1 cut(s) | 30 |
| FaiI | YATR | 3 cut(s) | 108, 110, 112 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 35 |
| GsuI | CTGGAG | 1 cut(s) | 103 |
| Hpy188III | TCNNGA | 2 cut(s) | 116, 144 |
| HpyAV | CCTTC | 1 cut(s) | 5 |
| HpyCH4V | TGCA | 3 cut(s) | 49, 62, 106 |
| LpnPI | CCDG | 4 cut(s) | 21, 48, 67, 109 |
| LweI | GCATC | 1 cut(s) | 36 |
| MboII | GAAGA | 2 cut(s) | 86, 139 |
| MnlI | CCTC | 2 cut(s) | 32, 45 |
| MseI | TTAA | 2 cut(s) | 21, 89 |
| SaqAI | TTAA | 2 cut(s) | 21, 89 |
| SetI | ASST | 2 cut(s) | 10, 97 |
| SfaNI | GCATC | 1 cut(s) | 36 |
| SgeI | CNNG | 6 cut(s) | 20, 69, 75, 94, 128, 136 |
| Tru1I | TTAA | 2 cut(s) | 21, 89 |
| Tru9I | TTAA | 2 cut(s) | 21, 89 |