Rorug07G0208100

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000030
Physical Location & Seq
Reverse (-)
18327795 .. 18332228
4434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0208100.1

Sequence Viewer

Length: 2433 bp
ATGAATTGGCCAACAAAATATCCGATTGAGTTCGATGAAGGATGGAGATGTATTGAGGAGGGGATTTCAAAGCTAAAGATGATTGCGGAAGGAACACATCGTCCATTCACAGCTGAAGAACACATGACTCTTCATACAGTGGTCTTTGACATGTGTACTCAGAGGCCCCCTTATGATCATAGTCAACAGCTTTATATCAAATACCGAAATATAGGTGAAGAATGCAGGGAGAGATGTGAAAAATACAGTGTGCCAATGAATCAGGATGATGAGTTTATTTTGTGGGACCTTGTCCATAGATGGGCACATTATAAAATTGCGGTTAATACAGTGCCGCGCATCTTTCATTATCTTCATCGCTACTATATGCCTCGTAGGTCACTTCCTGGAATAAATGACATTGTTGGACCTAACTGCTTCCGTGATTTGGTTTATCAGAAAGCAAATGTTAATGTGAGAGTTGCTGTAATCAATCTTATTCGTAAAGAACGTGAGGGAGAGAAAATTGACAGTGAACTTTTGAAGAAGGTGATAAATGCATATATTGAAATTGGAATGAAAAGAATGCATACTTATGAACAGGATTTTGAAGCACACATGCTAAGAGATACAGGTGAATACTATTCTCATAAAGCATCTAGTTGGATTTTGGCAGATTCATATGCAGACTATATGTGGAAGGCCGAGGGATGCTTGAGAAAGGAGAGGGATAGAGTTTCTCATTACATGCATTCAAGCAGTGAGCAGAAGCTGTTGGACAAAGTCCAACATGAGTTGTTTGATCCTCTTGCTGATGTATTTAAACAGCAAGTTGCTATAGAATGTACAAATTTGGTACACCTGGTTGAAGATGCTGCAGAAAACCAGGCTTCTTCTAATGAAGCAGACATGCTGGAACAGGTTCTTGTCAGGAAATTAAGTGAGGTGCATGTTAAGTATGTGAAATATGTCAATGATTGCTTCAAGAACTACTTTCACTTTCAGAGGGCTCTATTTGAGGCTTTTGGGGAATTTCGTGATAGAGTTTCTTCTGGATTTCTTGCTTTACTTAGAGAGGATAAGATGGAGGATCTTTTACTGATATATAAACTTTACCATAAAGTACCTGGAGGCCTGGAACCTTTTGCTAATTTATTTAAACAGTGTGTTACTGCTCAAGGTACAGCCTTGATTAAACAGGCTGATAATATTGCAAATAATCAGGCTTTAAATGGAGCAGGCATGCAGGAACAGGTCCTTATTAGACAATTACTTGAGCTGCACACTAAGCAAATGGCATATGTCAATGACTGCTTTATGAATCACTCCCTATTTCACAAAGCTCTTCATGAGGCTTTTAAGGTATTTTGCAATTCAAGTGCTGAAGTTCTTGCGGCATTCTGTGATAATATCCTGAAAATCAATGGGAATGACAAATTGTGTGGTCATCAGGACATAGAAGCAATTCTTGAGAAGGTTGTTACGACGTTGCTTCGCTATGTCAGTGACAGAGACCTCTTTGCAGAGTTCTACAGGATAAGACTTGCTCGCCGTCTTTTGTTTGATCGGAGTGCCAACAAGGACCTTGAAAATAGTATATTAACAAAGATGAAACAGCAATATGGGGAGCAGTTCACCTCAAAGATGGAGAGAATGGTTAAAGATGTCATTTTATCTCGGGAATGGCAGACAACATTCAAGGAATATCTTCGCAGCAATCCAGATGCAAATCTAGGGATGGATGTGACAGTCACTGTTCTTTCAACTGGTTTCTGGCCAAGTTATAAATCAATTGATGTTAACCTTCCTGCAGAGATGGTGAAGTGTGTTGAAGTTTTCAAGGGGTTCTATGCATCAAAAACCAAACACAGAAAACTTACATGGGTTTACTCATTGGGCACGTGCAACATCATTGGGAAGTTTGAGCCAAAAGCGATTGAATTAGTTGTGTCAACCCATCAGGCTGCTCTTCTGCTACTATTCAATACTGCTGATTCTTTGAGCTATTCAGAAATCGCAACTCAGTTAAATCTCAATCATGATGACTTGGTCAGAATCCTTCATTCACTTTCATGTGCCAAGTACAAGATCCTCATTAAGAATACAGATGCAATGACAATCTTGCCAAATGACGAATTTGAGTTCAATTCAAAGTTCACAGACACAATGAGGAGAATTAAGATTCCTCACCCCCAAGTAGTGGATGAAGCAAAGGAGGTAAAAAATGGTGTTAACAAAGACAGGCGATATGCTATTGAAGCTGCAATCGTGCGGATTATGAAGGAGCAGAAAGTTTTGGGTCATCAGCAATTGGTTCTGGAGTGTGTTGAGCAGTTGAGACAGACTTTCAAGCCTAACATCAAATCCATTAAGGGGCGCATTCAAAATCTCATCGATCGTGAGTACCTGGAAAGGGACCAGAAAAACTATAACTTGTACCGGTACGTTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

810

Amino Acids

94.66

Weight (kDa)

7.58

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin PF00888 26 - 270 1.4e-40 Cullin alpha solenoid domain
Cullin PF00888 346 - 554 2.6e-53 Cullin alpha solenoid domain
Cullin_AB PF26557 579 - 712 2.2e-41 Cullin alpha+beta domain
Cullin_Nedd8 PF10557 740 - 802 5.4e-25 Cullin protein neddylation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000030)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09630 FvH4_1g25481 FvH4_2g06390 FvH4_2g09470 FvH4_2g11952 FvH4_2g25151 FvH4_2g27901 FvH4_3g22392 FvH4_3g23130 FvH4_3g26751 FvH4_3g37244 FvH4_3g37260 FvH4_4g03329 FvH4_4g03350 FvH4_4g07720 FvH4_4g07721 FvH4_4g09520 FvH4_4g14081 FvH4_4g14090 FvH4_4g16060 FvH4_4g16061 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_5g19752 FvH4_5g19752 FvH4_5g19752 FvH4_5g33480 FvH4_6g25711 FvH4_6g25720 FvH4_6g40321 FvH4_6g40680 FvH4_6g40680 FvH4_7g05770 FvH4_7g05770 FvH4_7g06960 FvH4_7g07131 FvH4_7g07131 FvH4_7g07131 FvH4_7g07131 FvH4_7g08580 FvH4_7g10221 FvH4_7g12251 FvH4_7g12251 FvH4_7g12251 FvH4_c19g00020
prunus_persica Prupe.3G127800_v2.0.a1 Prupe.3G127900_v2.0.a1 Prupe.3G127900_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0313581 RchiOBHm_Chr1g0347831 RchiOBHm_Chr1g0372251 RchiOBHm_Chr2g0120671 RchiOBHm_Chr2g0120681 RchiOBHm_Chr2g0155601 RchiOBHm_Chr2g0161371 RchiOBHm_Chr2g0171911 RchiOBHm_Chr3g0451001 RchiOBHm_Chr3g0478081 RchiOBHm_Chr3g0485951 RchiOBHm_Chr4g0396211 RchiOBHm_Chr4g0396221 RchiOBHm_Chr4g0413121 RchiOBHm_Chr4g0417291 RchiOBHm_Chr4g0423011 RchiOBHm_Chr4g0424781 RchiOBHm_Chr4g0427951 RchiOBHm_Chr4g0432021 RchiOBHm_Chr5g0012051 RchiOBHm_Chr6g0268131 RchiOBHm_Chr6g0268141 RchiOBHm_Chr6g0281911 RchiOBHm_Chr6g0285311 RchiOBHm_Chr7g0189591 RchiOBHm_Chr7g0189601 RchiOBHm_Chr7g0224131
rosa_laevigata RLG00000000963 RLG00000000967 RLG00000001615 RLG00000002043 RLG00000002094 RLG00000002112 RLG00000002404 RLG00000004455 RLG00000005527 RLG00000005655 RLG00000006174 RLG00000007767 RLG00000008355 RLG00000008585 RLG00000008588 RLG00000008683 RLG00000008813 RLG00000009112 RLG00000009252 RLG00000009280 RLG00000009413 RLG00000012815 RLG00000012845 RLG00000013323 RLG00000013324 RLG00000015631 RLG00000017303 RLG00000018309 RLG00000018310 RLG00000018442 RLG00000018443 RLG00000018702 RLG00000019612 RLG00000021285 RLG00000021286 RLG00000022541 RLG00000023201 RLG00000023427 RLG00000029113 RLG00000029669 RLG00000030439 RLG00000030440 RLG00000033139 RLG00000034155 RLG00000034156 RLG00000034157 RLG00000034189 RLG00000034333 RLG00000034334 RLG00000034402 RLG00000034483
rosa_multiflora Rmu_co8408521.1_g000001 Rmu_co8451191.1_g000001 Rmu_co8509671.1_g000001 Rmu_co8512787.1_g000002 Rmu_sc0000077.1_g000042 Rmu_sc0000279.1_g000028 Rmu_sc0000280.1_g000005 Rmu_sc0000280.1_g000013 Rmu_sc0000319.1_g000017 Rmu_sc0000319.1_g000018 Rmu_sc0000361.1_g000007 Rmu_sc0000381.1_g000022 Rmu_sc0000642.1_g000014 Rmu_sc0000931.1_g000013 Rmu_sc0000982.1_g000033 Rmu_sc0001554.1_g000014 Rmu_sc0001992.1_g000027 Rmu_sc0002045.1_g000030 Rmu_sc0002106.1_g000002 Rmu_sc0003179.1_g000034 Rmu_sc0003422.1_g000009 Rmu_sc0004125.1_g000004 Rmu_sc0004999.1_g000015 Rmu_sc0005394.1_g000005 Rmu_sc0005712.1_g000015 Rmu_sc0006373.1_g000010 Rmu_sc0006872.1_g000002 Rmu_sc0006872.1_g000004 Rmu_sc0007367.1_g000011 Rmu_sc0007367.1_g000012 Rmu_sc0007491.1_g000005 Rmu_sc0007705.1_g000008 Rmu_sc0007705.1_g000009 Rmu_sc0007877.1_g000016 Rmu_sc0012512.1_g000006 Rmu_sc0021854.1_g000001 Rmu_sc0028193.1_g000003 Rmu_sc0032675.1_g000001 Rmu_ssc0000158.1_g000019 Rmu_ssc0000387.1_g000020
rosa_roxburghii Rroxscaffold_1G00009730 Rroxscaffold_1G00017900 Rroxscaffold_1G00031860 Rroxscaffold_1G00036000 Rroxscaffold_1G00036390 Rroxscaffold_1G00069020 Rroxscaffold_1G00071750 Rroxscaffold_2G00080160 Rroxscaffold_2G00080340 Rroxscaffold_2G00080790 Rroxscaffold_2G00085870 Rroxscaffold_2G00109300 Rroxscaffold_2G00113740 Rroxscaffold_2G00113750 Rroxscaffold_2G00113760 Rroxscaffold_2G00116400 Rroxscaffold_2G00116870 Rroxscaffold_2G00116880 Rroxscaffold_2G00118190 Rroxscaffold_2G00118200 Rroxscaffold_2G00118650 Rroxscaffold_2G00129110 Rroxscaffold_2G00134740 Rroxscaffold_2G00140920 Rroxscaffold_4G00285970 Rroxscaffold_4G00312160 Rroxscaffold_4G00322340 Rroxscaffold_5G00349950 Rroxscaffold_6G00430610 Rroxscaffold_7G00162630 Rroxscaffold_7G00162640 Rroxscaffold_7G00164320 Rroxscaffold_7G00214930
rosa_rugosa Rorug01G0012500 Rorug01G0018000 Rorug01G0019000 Rorug01G0019000 Rorug01G0070900 Rorug01G0094400 Rorug02G0043200 Rorug03G0329700 Rorug03G0329800 Rorug03G0329900 Rorug03G0332900 Rorug03G0343500 Rorug03G0343600 Rorug03G0343700 Rorug04G0032000 Rorug04G0065800 Rorug05G0102700 Rorug05G0106200 Rorug05G0256700 Rorug05G0256700 Rorug05G0439000 Rorug05G0540900 Rorug06G0055100 Rorug06G0060400 Rorug06G0170800 Rorug06G0184400 Rorug06G0439700 Rorug07G0182300 Rorug07G0182400 Rorug07G0182500 Rorug07G0208100 Rorug07G0208200 Rorug07G0208200 Rorug07G0283700.1 Rorug07G0283800
rosa_samantha Rh1AG090000 Rh1AG169000 Rh1BG060800 Rh1BG060900 Rh1BG070000 Rh1BG407100 Rh1BG407200 Rh1BG407400 Rh1BG407500 Rh1CG028100 Rh1CG028200 Rh1CG040100 Rh1CG050100 Rh1CG055500 Rh1CG156800 Rh1CG421400 Rh1CG421500 Rh1CG421600 Rh1DG029300 Rh1DG054600 Rh1DG054700 Rh1DG059400 Rh1DG177400 Rh1DG438200 Rh1DG438300 Rh1DG438500 Rh1DG438600 Rh1DG438700 Rh2AG286700 Rh2AG286800 Rh2AG286900 Rh2AG311900 Rh2AG322600 Rh2AG353300 Rh2AG353400 Rh2AG560900 Rh2AG642800 Rh2AG642900 Rh2AG643000 Rh2AG643100 Rh2AG647000 Rh2BG288800 Rh2BG317900 Rh2BG394800 Rh2BG394900 Rh2BG594000 Rh2BG657700 Rh2CG299000 Rh2CG299100 Rh2CG300200 Rh2DG102500 Rh2DG284000 Rh2DG312200 Rh2DG312300 Rh2DG336300 Rh2DG336400 Rh2DG337200 Rh2DG571300 Rh3AG246800 Rh3AG272200 Rh3BG121800 Rh3BG249400 Rh3BG265300 Rh3BG341500 Rh3BG348700 Rh3CG301300 Rh3CG307800 Rh3CG307900 Rh3CG308100 Rh3DG144400 Rh3DG251600 Rh3DG297700 Rh3DG301700 Rh3DG305100 Rh3DG340900 Rh4AG149000 Rh4AG248900 Rh4AG336100 Rh4BG044000 Rh4BG111400 Rh4BG133400 Rh4BG182300 Rh4BG219600 Rh4BG219700 Rh4BG254300 Rh4BG388300 Rh4BG388600 Rh4CG126200 Rh4CG155800 Rh4CG257000 Rh4CG402900 Rh4CG403000 Rh4DG005400 Rh4DG057400 Rh4DG057500 Rh4DG057700 Rh4DG120700 Rh4DG280800 Rh4DG338600 Rh5AG057600 Rh5AG057700 Rh5AG057800 Rh5AG284700 Rh5AG284800 Rh5AG305600 Rh5AG364300 Rh5AG365100 Rh5AG365200 Rh5AG516000 Rh5AG516200 Rh5BG157600 Rh5BG235200 Rh5BG235400 Rh5BG308900 Rh5BG373800 Rh5BG414500 Rh5BG538800 Rh5CG102300 Rh5CG248400 Rh5CG249300 Rh5CG249400 Rh5CG326300 Rh5CG326400 Rh5CG366400 Rh5CG366500 Rh5CG439100 Rh5CG439200 Rh5CG497700 Rh5CG562500 Rh5CG567300 Rh5DG179600 Rh5DG179700 Rh5DG239900 Rh5DG299700 Rh5DG319400 Rh5DG319500 Rh6AG173400 Rh6AG175300 Rh6AG314800 Rh6AG405000 Rh6BG067300 Rh6BG067400 Rh6BG075300 Rh6BG101700 Rh6BG134300 Rh6BG324500 Rh6CG087200 Rh6CG132400 Rh6CG151800 Rh6CG151900 Rh6CG200100 Rh6CG205500 Rh6CG419400 Rh6CG419500 Rh6DG081400 Rh6DG119900 Rh6DG120000 Rh6DG191500 Rh6DG199900 Rh6DG251900 Rh6DG252000 Rh6DG498400 Rh7AG274800 Rh7AG283100 Rh7AG352600 Rh7AG364900 Rh7AG386800 Rh7BG321800 Rh7BG341100 Rh7CG294000 Rh7CG368900 Rh7CG369900 Rh7CG383700 Rh7CG406000 Rh7CG406100 Rh7DG106300 Rh7DG106400 Rh7DG286300 Rh7DG346900 Rh7DG353400 Rh7DG360000 Rh7DG361700 Rh7DG362200 Rh7DG362600 Rh7DG362900 Rh7DG363400
rosa_wichuraiana Rw0G000990 Rw0G001740 Rw0G002300 Rw0G002310 Rw0G007890 Rw0G007900 Rw0G023450 Rw0G023460 Rw1G000300 Rw1G007140 Rw1G007170 Rw1G008970 Rw1G010460 Rw1G039090 Rw1G039100 Rw2G012530 Rw2G016610 Rw2G023010 Rw2G031730 Rw3G014310 Rw4G008550 Rw4G010240 Rw4G012160 Rw4G024150 Rw5G009090 Rw5G047250 Rw5G048500 Rw5G050510 Rw6G006430 Rw6G006440 Rw6G006450 Rw6G013330 Rw6G016560 Rw6G021330 Rw6G024630 Rw6G035440 Rw6G041540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 312, 1764
AccB7I CCANNNNNTGG 1 cut(s) 2179
AccII CGCG 1 cut(s) 337
AciI CCGC 5 cut(s) 86, 320, 335, 1373, 2251
AclWI GGATC 3 cut(s) 776, 1077, 2062
AcoI YGGCCR 2 cut(s) 8, 1754
AcsI RAATTY 3 cut(s) 829, 1010, 2114
AcuI CTGAAG 2 cut(s) 135, 1383
AcvI CACGTG 1 cut(s) 1881
AfaI GTAC 9 cut(s) 157, 826, 837, 1104, 1162, 2063, 2384, 2417, 2423
AfiI CCNNNNNNNGG 5 cut(s) 301, 427, 2179, 2352, 2392
AflIII ACRYGT 1 cut(s) 150
AgeI ACCGGT 1 cut(s) 2418
AjnI CCWGG 6 cut(s) 385, 840, 864, 1105, 1113, 2385
AloI GAACNNNNNNTCC 2 cut(s) 85, 117
AluBI AGCT 8 cut(s) 73, 113, 190, 751, 1258, 1322, 1983, 2240
AluI AGCT 8 cut(s) 73, 113, 190, 751, 1258, 1322, 1983, 2240
Alw26I GTCTC 2 cut(s) 1485, 2311
AlwI GGATC 3 cut(s) 776, 1077, 2062
AlwNI CAGNNNCTG 2 cut(s) 751, 1733
Ama87I CYCGRG 1 cut(s) 1656
AoxI GGCC 5 cut(s) 8, 164, 681, 1111, 1754
ApeKI GCWGC 5 cut(s) 854, 1258, 1692, 1943, 2240
ApoI RAATTY 3 cut(s) 829, 1010, 2114
AsiGI ACCGGT 1 cut(s) 2418
Asp700I GAANNNNTTC 3 cut(s) 900, 1443, 1686
AspLEI GCGC 2 cut(s) 339, 2358
AspS9I GGNCC 6 cut(s) 165, 286, 407, 1234, 1561, 2395
AsuHPI GGTGA 6 cut(s) 227, 541, 626, 1606, 1810, 2159
AvaI CYCGRG 1 cut(s) 1656
AvaII GGWCC 5 cut(s) 286, 407, 1234, 1561, 2395
BaeGI GKGCMC 2 cut(s) 307, 1880
BalI TGGCCA 2 cut(s) 10, 1756
BanII GRGCYC 1 cut(s) 991
BbrPI CACGTG 1 cut(s) 1881
BbvI GCAGC 5 cut(s) 841, 1245, 1704, 1930, 2227
BccI CCATC 7 cut(s) 36, 294, 1057, 1618, 1711, 1789, 1944
BceAI ACGGC 1 cut(s) 1515
BciT130I CCWGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
BclI TGATCA 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 1485, 2311
BfaI CTAG 2 cut(s) 639, 1712
BfmI CTRYAG 4 cut(s) 816, 855, 1510, 1788
BisI GCNGC 7 cut(s) 335, 855, 1259, 1374, 1693, 1944, 2241
BlsI GCNGC 7 cut(s) 336, 856, 1260, 1375, 1694, 1945, 2242
Bme1390I CCNGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
Bme18I GGWCC 5 cut(s) 286, 407, 1234, 1561, 2395
BmeT110I CYCGRG 1 cut(s) 1656
BmgT120I GGNCC 6 cut(s) 165, 286, 407, 1234, 1561, 2395
BmiI GGNNCC 4 cut(s) 167, 287, 1119, 2396
BmrFI CCNGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
BmsI GCATC 7 cut(s) 348, 644, 680, 841, 1693, 1841, 2077
BpmI CTGGAG 2 cut(s) 1128, 2318
BpuEI CTTGAG 4 cut(s) 715, 1140, 1274, 1469
Bsa29I ATCGAT 1 cut(s) 2373
BsaAI YACGTR 1 cut(s) 1881
BsaBI GATNNNNATC 1 cut(s) 1707
BsaI GGTCTC 1 cut(s) 1485
BsaJI CCNNGG 1 cut(s) 684
BsaWI WCCGGW 1 cut(s) 2418
Bsc4I CCNNNNNNNGG 5 cut(s) 301, 427, 2179, 2352, 2392
Bse118I RCCGGY 1 cut(s) 2418
Bse1I ACTGG 1 cut(s) 1750
Bse3DI GCAATG 1 cut(s) 2097
Bse8I GATNNNNATC 1 cut(s) 1707
BseBI CCWGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
BseCI ATCGAT 1 cut(s) 2373
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 6 cut(s) 47, 271, 695, 1722, 1726, 2188
BseJI GATNNNNATC 1 cut(s) 1707
BseLI CCNNNNNNNGG 5 cut(s) 301, 427, 2179, 2352, 2392
BseMI GCAATG 1 cut(s) 2097
BseMII CTCAG 2 cut(s) 173, 2015
BseNI ACTGG 1 cut(s) 1750
BseRI GAGGAG 2 cut(s) 71, 2164
BseSI GKGCMC 2 cut(s) 307, 1880
BseXI GCAGC 5 cut(s) 841, 1245, 1704, 1930, 2227
BsgI GTGCAG 1 cut(s) 1244
Bsh1236I CGCG 1 cut(s) 337
Bsh1285I CGRYCG 1 cut(s) 2377
BshFI GGCC 5 cut(s) 10, 166, 683, 1113, 1756
BshTI ACCGGT 1 cut(s) 2418
BshVI ATCGAT 1 cut(s) 2373
BsiEI CGRYCG 1 cut(s) 2377
BsiHKCI CYCGRG 1 cut(s) 1656
BsiSI CCGG 1 cut(s) 2419
BslFI GGGAC 2 cut(s) 299, 2408
BslI CCNNNNNNNGG 5 cut(s) 301, 427, 2179, 2352, 2392
BsmAI GTCTC 2 cut(s) 1485, 2311
BsmFI GGGAC 2 cut(s) 299, 2408
BsmI GAATGC 5 cut(s) 227, 570, 730, 1376, 2358
BsnI GGCC 5 cut(s) 10, 166, 683, 1113, 1756
Bso31I GGTCTC 1 cut(s) 1485
BsoBI CYCGRG 1 cut(s) 1656
Bsp1286I GDGCHC 3 cut(s) 307, 991, 1880
Bsp1407I TGTACA 1 cut(s) 824
Bsp143I GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
BspACI CCGC 5 cut(s) 86, 320, 335, 1373, 2251
BspANI GGCC 5 cut(s) 10, 166, 683, 1113, 1756
BspCNI CTCAG 2 cut(s) 172, 2014
BspDI ATCGAT 1 cut(s) 2373
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 2 cut(s) 1327, 2017
BspLI GGNNCC 4 cut(s) 167, 287, 1119, 2396
BspMAI CTGCAG 2 cut(s) 859, 1792
BspPI GGATC 3 cut(s) 776, 1077, 2062
BspQI GCTCTTC 2 cut(s) 1329, 1953
BspTNI GGTCTC 1 cut(s) 1485
BsrDI GCAATG 1 cut(s) 2097
BsrFI RCCGGY 1 cut(s) 2418
BsrGI TGTACA 1 cut(s) 824
BsrI ACTGG 1 cut(s) 1750
BssAI RCCGGY 1 cut(s) 2418
BssECI CCNNGG 1 cut(s) 684
BssMI GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
Bst2UI CCWGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
Bst4CI ACNGT 7 cut(s) 139, 248, 331, 512, 1143, 1729, 1735
Bst6I CTCTTC 3 cut(s) 135, 1329, 1953
BstAUI TGTACA 1 cut(s) 824
BstBAI YACGTR 1 cut(s) 1881
BstC8I GCNNGC 3 cut(s) 1219, 1223, 1528
BstDEI CTNAG 5 cut(s) 159, 602, 1049, 1266, 2001
BstF5I GGATG 6 cut(s) 47, 271, 695, 1722, 1726, 2188
BstFNI CGCG 1 cut(s) 337
BstHHI GCGC 2 cut(s) 339, 2358
BstKTI GATC 6 cut(s) 178, 784, 1072, 1546, 2070, 2377
BstMAI GTCTC 2 cut(s) 1485, 2311
BstMBI GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
BstMCI CGRYCG 1 cut(s) 2377
BstMWI GCNNNNNNNGC 2 cut(s) 1267, 2237
BstNI CCWGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
BstNSI RCATGY 6 cut(s) 154, 601, 730, 892, 932, 1225
BstSCI CCNGG 6 cut(s) 385, 840, 864, 1105, 1113, 2385
BstSFI CTRYAG 4 cut(s) 816, 855, 1510, 1788
BstSLI GKGCMC 2 cut(s) 307, 1880
BstUI CGCG 1 cut(s) 337
BstV1I GCAGC 5 cut(s) 841, 1245, 1704, 1930, 2227
BstX2I RGATCY 2 cut(s) 1069, 2067
BstYI RGATCY 2 cut(s) 1069, 2067
Bsu15I ATCGAT 1 cut(s) 2373
BsuRI GGCC 5 cut(s) 10, 166, 683, 1113, 1756
BsuTUI ATCGAT 1 cut(s) 2373
BtgZI GCGATG 1 cut(s) 341
BtsCI GGATG 6 cut(s) 47, 271, 695, 1722, 1726, 2188
BtsI GCAGTG 1 cut(s) 745
BtsIMutI CAGTG 8 cut(s) 144, 253, 336, 517, 745, 1148, 1489, 1731
Cac8I GCNNGC 3 cut(s) 1219, 1223, 1528
CaiI CAGNNNCTG 2 cut(s) 751, 1733
CciI TCATGA 2 cut(s) 1327, 2017
CfoI GCGC 2 cut(s) 339, 2358
Cfr10I RCCGGY 1 cut(s) 2418
Cfr13I GGNCC 6 cut(s) 165, 286, 407, 1234, 1561, 2395
ClaI ATCGAT 1 cut(s) 2373
CsiI ACCWGGT 1 cut(s) 840
Csp6I GTAC 9 cut(s) 156, 825, 836, 1103, 1161, 2062, 2383, 2416, 2422
CspAI ACCGGT 1 cut(s) 2418
CspCI CAANNNNNGTGG 2 cut(s) 1402, 1437
CviQI GTAC 9 cut(s) 156, 825, 836, 1103, 1161, 2062, 2383, 2416, 2422
DdeI CTNAG 5 cut(s) 159, 602, 1049, 1266, 2001
DpnI GATC 6 cut(s) 177, 783, 1071, 1545, 2069, 2376
DpnII GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
DraI TTTAAA 3 cut(s) 802, 1138, 1209
EaeI YGGCCR 2 cut(s) 8, 1754
Eam1104I CTCTTC 3 cut(s) 135, 1329, 1953
EarI CTCTTC 3 cut(s) 135, 1329, 1953
Eco147I AGGCCT 1 cut(s) 1113
Eco24I GRGCYC 1 cut(s) 991
Eco31I GGTCTC 1 cut(s) 1485
Eco47I GGWCC 5 cut(s) 286, 407, 1234, 1561, 2395
Eco57I CTGAAG 2 cut(s) 135, 1383
Eco72I CACGTG 1 cut(s) 1881
Eco88I CYCGRG 1 cut(s) 1656
EcoO109I RGGNCCY 4 cut(s) 165, 286, 1234, 1561
EcoRII CCWGG 6 cut(s) 385, 840, 864, 1105, 1113, 2385
EcoT22I ATGCAT 4 cut(s) 541, 570, 732, 1834
EcoT38I GRGCYC 1 cut(s) 991
FalI AAGNNNNNCTT 4 cut(s) 956, 988, 1431, 1463
FaqI GGGAC 2 cut(s) 299, 2408
FauNDI CATATG 2 cut(s) 661, 1279
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 7 cut(s) 335, 855, 1259, 1374, 1693, 1944, 2241
FokI GGATG 6 cut(s) 54, 278, 702, 1729, 1733, 2195
FriOI GRGCYC 1 cut(s) 991
Fsp4HI GCNGC 7 cut(s) 335, 855, 1259, 1374, 1693, 1944, 2241
FspBI CTAG 2 cut(s) 639, 1712
GlaI GCGC 2 cut(s) 338, 2357
GluI GCNGC 7 cut(s) 335, 855, 1259, 1374, 1693, 1944, 2241
GsuI CTGGAG 2 cut(s) 1128, 2318
HaeIII GGCC 5 cut(s) 10, 166, 683, 1113, 1756
HapII CCGG 1 cut(s) 2419
HhaI GCGC 2 cut(s) 339, 2358
Hin6I GCGC 2 cut(s) 337, 2356
HinP1I GCGC 2 cut(s) 337, 2356
HincII GTYRAC 4 cut(s) 185, 1780, 1932, 2212
HindII GTYRAC 4 cut(s) 185, 1780, 1932, 2212
HinfI GANTC 7 cut(s) 127, 259, 656, 1300, 1973, 2034, 2161
HpaI GTTAAC 2 cut(s) 1780, 2212
HpaII CCGG 1 cut(s) 2419
HphI GGTGA 6 cut(s) 227, 541, 626, 1606, 1810, 2159
Hpy188I TCNGA 7 cut(s) 24, 162, 438, 984, 1548, 1990, 2033
Hpy99I CGWCG 1 cut(s) 1468
HpyAV CCTTC 8 cut(s) 32, 83, 520, 673, 1447, 1793, 2048, 2254
HpyCH4III ACNGT 7 cut(s) 139, 248, 331, 512, 1143, 1729, 1735
HpyCH4IV ACGT 4 cut(s) 490, 1466, 1880, 2424
HpyF10VI GCNNNNNNNGC 2 cut(s) 1267, 2237
HpyF3I CTNAG 5 cut(s) 159, 602, 1049, 1266, 2001
HpySE526I ACGT 4 cut(s) 490, 1466, 1880, 2424
HspAI GCGC 2 cut(s) 337, 2356
Ksp22I TGATCA 1 cut(s) 175
KspAI GTTAAC 2 cut(s) 1780, 2212
Kzo9I GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
LguI GCTCTTC 2 cut(s) 1329, 1953
LmnI GCTCC 3 cut(s) 1214, 1606, 2263
Lsp1109I GCAGC 5 cut(s) 841, 1245, 1704, 1930, 2227
LweI GCATC 7 cut(s) 348, 644, 680, 841, 1693, 1841, 2077
MabI ACCWGGT 1 cut(s) 840
MaeI CTAG 2 cut(s) 639, 1712
MaeII ACGT 4 cut(s) 490, 1466, 1880, 2424
MaeIII GTNAC 6 cut(s) 378, 1147, 1459, 1484, 1723, 1729
MalI GATC 6 cut(s) 177, 783, 1071, 1545, 2069, 2376
MboI GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
MfeI CAATTG 2 cut(s) 1770, 2288
MflI RGATCY 2 cut(s) 1069, 2067
MhlI GDGCHC 3 cut(s) 307, 991, 1880
MlsI TGGCCA 2 cut(s) 10, 1756
MluNI TGGCCA 2 cut(s) 10, 1756
MlyI GAGTC 1 cut(s) 121
MmeI TCCRAC 4 cut(s) 385, 623, 735, 790
Mox20I TGGCCA 2 cut(s) 10, 1756
Mph1103I ATGCAT 4 cut(s) 541, 570, 732, 1834
MroXI GAANNNNTTC 3 cut(s) 900, 1443, 1686
MscI TGGCCA 2 cut(s) 10, 1756
MslI CAYNNNNRTG 2 cut(s) 573, 2050
Msp20I TGGCCA 2 cut(s) 10, 1756
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 1 cut(s) 2419
MspR9I CCNGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
MunI CAATTG 2 cut(s) 1770, 2288
Mva1269I GAATGC 5 cut(s) 227, 570, 730, 1376, 2358
MvaI CCWGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
MvnI CGCG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 1267, 2237
NdeI CATATG 2 cut(s) 661, 1279
NdeII GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
NlaIV GGNNCC 4 cut(s) 167, 287, 1119, 2396
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 4 cut(s) 378, 1484, 1723, 1729
NsiI ATGCAT 4 cut(s) 541, 570, 732, 1834
NspI RCATGY 6 cut(s) 154, 601, 730, 892, 932, 1225
PaeI GCATGC 1 cut(s) 1225
PagI TCATGA 2 cut(s) 1327, 2017
PceI AGGCCT 1 cut(s) 1113
PciI ACATGT 1 cut(s) 150
PciSI GCTCTTC 2 cut(s) 1329, 1953
PcsI WCGNNNNNNNCGW 1 cut(s) 487
PctI GAATGC 5 cut(s) 227, 570, 730, 1376, 2358
PdmI GAANNNNTTC 3 cut(s) 900, 1443, 1686
PfeI GAWTC 6 cut(s) 259, 656, 1300, 1973, 2034, 2161
PflFI GACNNNGTC 3 cut(s) 290, 761, 2027
PflMI CCANNNNNTGG 1 cut(s) 2179
PfoI TCCNGGA 1 cut(s) 385
PinAI ACCGGT 1 cut(s) 2418
PkrI GCNGC 7 cut(s) 336, 856, 1260, 1375, 1694, 1945, 2242
Ple19I CGATCG 1 cut(s) 2377
PleI GAGTC 1 cut(s) 121
PmaCI CACGTG 1 cut(s) 1881
PmlI CACGTG 1 cut(s) 1881
PpsI GAGTC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 1881
PpuMI RGGWCCY 3 cut(s) 286, 1234, 1561
PscI ACATGT 1 cut(s) 150
PsiI TTATAA 2 cut(s) 312, 1764
Psp5II RGGWCCY 3 cut(s) 286, 1234, 1561
Psp6I CCWGG 6 cut(s) 385, 840, 864, 1105, 1113, 2385
PspCI CACGTG 1 cut(s) 1881
PspGI CCWGG 6 cut(s) 385, 840, 864, 1105, 1113, 2385
PspN4I GGNNCC 4 cut(s) 167, 287, 1119, 2396
PspPI GGNCC 6 cut(s) 165, 286, 407, 1234, 1561, 2395
PspPPI RGGWCCY 3 cut(s) 286, 1234, 1561
PstI CTGCAG 2 cut(s) 859, 1792
PstNI CAGNNNCTG 2 cut(s) 751, 1733
PsuI RGATCY 2 cut(s) 1069, 2067
PsyI GACNNNGTC 3 cut(s) 290, 761, 2027
PvuI CGATCG 1 cut(s) 2377
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 9 cut(s) 157, 826, 837, 1104, 1162, 2063, 2384, 2417, 2423
RsaNI GTAC 9 cut(s) 156, 825, 836, 1103, 1161, 2062, 2383, 2416, 2422
RseI CAYNNNNRTG 2 cut(s) 573, 2050
SapI GCTCTTC 2 cut(s) 1329, 1953
SatI GCNGC 7 cut(s) 335, 855, 1259, 1374, 1693, 1944, 2241
Sau3AI GATC 6 cut(s) 175, 781, 1069, 1543, 2067, 2374
Sau96I GGNCC 6 cut(s) 165, 286, 407, 1234, 1561, 2395
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 6 cut(s) 387, 842, 866, 1107, 1115, 2387
SduI GDGCHC 3 cut(s) 307, 991, 1880
SexAI ACCWGGT 1 cut(s) 840
SfaNI GCATC 7 cut(s) 348, 644, 680, 841, 1693, 1841, 2077
SfcI CTRYAG 4 cut(s) 816, 855, 1510, 1788
SinI GGWCC 5 cut(s) 286, 407, 1234, 1561, 2395
SmiMI CAYNNNNRTG 2 cut(s) 573, 2050
SmlI CTYRAG 4 cut(s) 694, 1155, 1253, 1448
SmoI CTYRAG 4 cut(s) 694, 1155, 1253, 1448
SphI GCATGC 1 cut(s) 1225
SseBI AGGCCT 1 cut(s) 1113
SsiI CCGC 5 cut(s) 86, 320, 335, 1373, 2251
SspI AATATT 1 cut(s) 1189
SspMI CTAG 2 cut(s) 639, 1712
StuI AGGCCT 1 cut(s) 1113
StyD4I CCNGG 6 cut(s) 385, 840, 864, 1105, 1113, 2385
TaaI ACNGT 7 cut(s) 139, 248, 331, 512, 1143, 1729, 1735
TaiI ACGT 4 cut(s) 493, 1469, 1883, 2427
TaqI TCGA 2 cut(s) 33, 2373
TatI WGTACW 3 cut(s) 155, 824, 2061
TauI GCSGC 2 cut(s) 337, 1376
TfiI GAWTC 6 cut(s) 259, 656, 1300, 1973, 2034, 2161
TscAI CASTG 8 cut(s) 144, 253, 336, 517, 745, 1148, 1489, 1738
TseFI GTSAC 4 cut(s) 378, 1484, 1723, 1729
TseI GCWGC 5 cut(s) 854, 1258, 1692, 1943, 2240
Tsp45I GTSAC 4 cut(s) 378, 1484, 1723, 1729
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 8 cut(s) 144, 253, 336, 517, 745, 1148, 1489, 1738
Tth111I GACNNNGTC 3 cut(s) 290, 761, 2027
Van91I CCANNNNNTGG 1 cut(s) 2179
VpaK11BI GGWCC 5 cut(s) 286, 407, 1234, 1561, 2395
XapI RAATTY 3 cut(s) 829, 1010, 2114
XceI RCATGY 6 cut(s) 154, 601, 730, 892, 932, 1225
XcmI CCANNNNNNNNNTGG 1 cut(s) 1103
XmnI GAANNNNTTC 3 cut(s) 900, 1443, 1686
XspI CTAG 2 cut(s) 639, 1712
Zsp2I ATGCAT 4 cut(s) 541, 570, 732, 1834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.