Rw5G048500

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000030
Physical Location & Seq
Forward (+)
84350750 .. 84354135
3386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048500.1

Sequence Viewer

Length: 2295 bp
ATGGTTTCGACGACTGAGGAGGGAGGCACGGAGCTAATAAGTAGTGACAGCGGCATGCAAATTGACGGGACTCAAGTAGCGGCTGTTGCAAATAACTCGTTGCTTACATATAAAGCATCATCGTCGGACACGATGGTACATGAGAAAGAGACAGTTTCCTCCCTTGAAGACTCCATTGGACATGAGAATGGTGGTGAAAAATTGGGTAAGTGTGATGGGAAAACTGGAGATTGTCCGGTGAGTCCTATAGTAGGTCGACGCATGAGGGAATTTAGAGCAATATGTACTGGTTTGTCAGAGGAGAAGCAAGTAGCTATTGATGCAAGTGGTTTTGGGGCTATATTCACGTTTAGGTGTAGCGAACTTAACGTGCTTCTATGCCACATGATGTGCAACAACTTTGATGTTGAAAACACAACAATCAAGATTCACGGGAGACATTTAACAGTCACCCCTGCAGACTTCCGCCATGTACTGAGTATAAGGGATGGTGGGGCTGACGTAGATTTAACAGGAAGCTTATCTGAACCGGACATTGTAGAGTTGAAGTCCAAGTTTTGTGGTGTTGATGAGGAAATAAGCTTGGATGCTGTGGGGAAGATGGTGGCCCAATCAGAAACTGCTGATGATACATTTATAATAAGTTTTTGTCTCTATGCCCTGTCAACTATGATTTTGCCGAGTGAAAGTGATGCAGTGGATCCCAGGCTTCTTCTCGCCGTAAAGGATCCGAAAGCGATTAGCAAAAAAAACTGGGCTTCCTTTGCATTTAGAAAGCTTGTTGAAGGACTTGGAAGGTATAAGAATGGGGTGTCCTCAAATGTGTATGGGTGTATCGCATTTCTGCAGCTGTTTTATTTGGATACAATGCTAATGAAGTTCCTTGTTGTTCCGACATACACATCTCCGGTACTCTGTTGGACTAACAAGCAGGCAAGAATTATGTATGACCAGGCAGAGGCTAATGGTGGGTATGACTCGACTGGTATAATAGCGACCAAGCGTTGGTCGACAACTCGATTCCTTGATAATGCCGGATCTGTTGATGCAAGTACAGGACTGAAAACAACTATTGTTGAAGATCTAGACAAGGTCAGGAACGATGTTGATGCTTTCAAATGCGAATTTGAGGAGGTTAGATCTTGCATATCTCATATAGAACCGGACATGGTGGGCCTCCGCAATGCTATTATGACACTGCAAACATCTATTTGTGAAATGAGGGATAGAGGGATGAGTAACATGGTTGTAGAAGTGATGAAGGAAGTCCTTGATGAGGAGGGTTCGTTTGAGCAGAAGGGGAGGGATGAATCAGATATGTACGAAGATGACATGTGTCCCAGTCAGCGGGGATCGCCGAGTGTCGTTGGGCCGCATAAGTCTAATATGGATAATGTTAAGGACAAAAGCTTGTCATTGAAGCGAAAGTCAGACAATTGTGTTGATGATGACAACATGGTCAAATTATGTGCAGTTGGTGATATATCCAACAGCGAGTCATTTCTGATTACTTACTTGTTCAACAAGGTGATGGCTGAGTCTGACAGTCTTGCAAGCAAGGAGGTTGCAAGGTATGGAGATCAGAGTATCTCTAGATGGGACCTCTGTTGCCTGTCACCGATGCAGTCTATCACAAGCGAGGAACGTGCTAAGGACTACACAAGTGGTAGTGTTGTAGACGGGTTGATATCATCTGTGGTGACTATATGTCGCCTTCGTAGATATAGCGGCCGATTGGGTTCTTGCAAGCAGATTTTCTTCCCTCTGCGCGACGGTGTTGTGGATAGCTGGTTTTTGGTGGTTTTGAATCTGGAGGTGGGGGAATTAGAGATTTGGGACAGCTGCCCCGACATGAATGCCAGACAGCGTCGTGAGTCTTATGGAAATGCTGTGGTGGAGCTATTTCAGAATGTTTTTAGTAATGAAATAAGATTGTTTCCCGCACTTTATGCAAGACTAGCTTCAGTGTCTATAGTTTATCCTAAAGAGGGCCCGACCAACCCAAAGACTCAGGATTCCGGTATATTTGTAATCCGGAACATGCAGTATTACCGGAAACGCTGGTTTGAAGGGTTCAACTCCGGTGACCAAAGGATCCGCCTTGCTGTTGATATAGTGAATCACCCAATGAATGCAGTGCGTGTCTCTGTATGGGCAGCAGCAGCTCAGGAAACTCCAATTGATGCTAAATCATTTGGACGTGCAGCATACAACGGGAACTTCATCCCTAATTGCAATATTCCACTTGATATAGCAGGAATGAAATTCAAGGCTCGTCGGACTTCGCGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

764

Amino Acids

84.71

Weight (kDa)

5.18

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 583 - 686 1.7e-06 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000030)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09630 FvH4_1g25481 FvH4_2g06390 FvH4_2g09470 FvH4_2g11952 FvH4_2g25151 FvH4_2g27901 FvH4_3g22392 FvH4_3g23130 FvH4_3g26751 FvH4_3g37244 FvH4_3g37260 FvH4_4g03329 FvH4_4g03350 FvH4_4g07720 FvH4_4g07721 FvH4_4g09520 FvH4_4g14081 FvH4_4g14090 FvH4_4g16060 FvH4_4g16061 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_4g21890 FvH4_5g19752 FvH4_5g19752 FvH4_5g19752 FvH4_5g33480 FvH4_6g25711 FvH4_6g25720 FvH4_6g40321 FvH4_6g40680 FvH4_6g40680 FvH4_7g05770 FvH4_7g05770 FvH4_7g06960 FvH4_7g07131 FvH4_7g07131 FvH4_7g07131 FvH4_7g07131 FvH4_7g08580 FvH4_7g10221 FvH4_7g12251 FvH4_7g12251 FvH4_7g12251 FvH4_c19g00020
prunus_persica Prupe.3G127800_v2.0.a1 Prupe.3G127900_v2.0.a1 Prupe.3G127900_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261000_v2.0.a1 Prupe.4G261400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0313581 RchiOBHm_Chr1g0347831 RchiOBHm_Chr1g0372251 RchiOBHm_Chr2g0120671 RchiOBHm_Chr2g0120681 RchiOBHm_Chr2g0155601 RchiOBHm_Chr2g0161371 RchiOBHm_Chr2g0171911 RchiOBHm_Chr3g0451001 RchiOBHm_Chr3g0478081 RchiOBHm_Chr3g0485951 RchiOBHm_Chr4g0396211 RchiOBHm_Chr4g0396221 RchiOBHm_Chr4g0413121 RchiOBHm_Chr4g0417291 RchiOBHm_Chr4g0423011 RchiOBHm_Chr4g0424781 RchiOBHm_Chr4g0427951 RchiOBHm_Chr4g0432021 RchiOBHm_Chr5g0012051 RchiOBHm_Chr6g0268131 RchiOBHm_Chr6g0268141 RchiOBHm_Chr6g0281911 RchiOBHm_Chr6g0285311 RchiOBHm_Chr7g0189591 RchiOBHm_Chr7g0189601 RchiOBHm_Chr7g0224131
rosa_laevigata RLG00000000963 RLG00000000967 RLG00000001615 RLG00000002043 RLG00000002094 RLG00000002112 RLG00000002404 RLG00000004455 RLG00000005527 RLG00000005655 RLG00000006174 RLG00000007767 RLG00000008355 RLG00000008585 RLG00000008588 RLG00000008683 RLG00000008813 RLG00000009112 RLG00000009252 RLG00000009280 RLG00000009413 RLG00000012815 RLG00000012845 RLG00000013323 RLG00000013324 RLG00000015631 RLG00000017303 RLG00000018309 RLG00000018310 RLG00000018442 RLG00000018443 RLG00000018702 RLG00000019612 RLG00000021285 RLG00000021286 RLG00000022541 RLG00000023201 RLG00000023427 RLG00000029113 RLG00000029669 RLG00000030439 RLG00000030440 RLG00000033139 RLG00000034155 RLG00000034156 RLG00000034157 RLG00000034189 RLG00000034333 RLG00000034334 RLG00000034402 RLG00000034483
rosa_multiflora Rmu_co8408521.1_g000001 Rmu_co8451191.1_g000001 Rmu_co8509671.1_g000001 Rmu_co8512787.1_g000002 Rmu_sc0000077.1_g000042 Rmu_sc0000279.1_g000028 Rmu_sc0000280.1_g000005 Rmu_sc0000280.1_g000013 Rmu_sc0000319.1_g000017 Rmu_sc0000319.1_g000018 Rmu_sc0000361.1_g000007 Rmu_sc0000381.1_g000022 Rmu_sc0000642.1_g000014 Rmu_sc0000931.1_g000013 Rmu_sc0000982.1_g000033 Rmu_sc0001554.1_g000014 Rmu_sc0001992.1_g000027 Rmu_sc0002045.1_g000030 Rmu_sc0002106.1_g000002 Rmu_sc0003179.1_g000034 Rmu_sc0003422.1_g000009 Rmu_sc0004125.1_g000004 Rmu_sc0004999.1_g000015 Rmu_sc0005394.1_g000005 Rmu_sc0005712.1_g000015 Rmu_sc0006373.1_g000010 Rmu_sc0006872.1_g000002 Rmu_sc0006872.1_g000004 Rmu_sc0007367.1_g000011 Rmu_sc0007367.1_g000012 Rmu_sc0007491.1_g000005 Rmu_sc0007705.1_g000008 Rmu_sc0007705.1_g000009 Rmu_sc0007877.1_g000016 Rmu_sc0012512.1_g000006 Rmu_sc0021854.1_g000001 Rmu_sc0028193.1_g000003 Rmu_sc0032675.1_g000001 Rmu_ssc0000158.1_g000019 Rmu_ssc0000387.1_g000020
rosa_roxburghii Rroxscaffold_1G00009730 Rroxscaffold_1G00017900 Rroxscaffold_1G00031860 Rroxscaffold_1G00036000 Rroxscaffold_1G00036390 Rroxscaffold_1G00069020 Rroxscaffold_1G00071750 Rroxscaffold_2G00080160 Rroxscaffold_2G00080340 Rroxscaffold_2G00080790 Rroxscaffold_2G00085870 Rroxscaffold_2G00109300 Rroxscaffold_2G00113740 Rroxscaffold_2G00113750 Rroxscaffold_2G00113760 Rroxscaffold_2G00116400 Rroxscaffold_2G00116870 Rroxscaffold_2G00116880 Rroxscaffold_2G00118190 Rroxscaffold_2G00118200 Rroxscaffold_2G00118650 Rroxscaffold_2G00129110 Rroxscaffold_2G00134740 Rroxscaffold_2G00140920 Rroxscaffold_4G00285970 Rroxscaffold_4G00312160 Rroxscaffold_4G00322340 Rroxscaffold_5G00349950 Rroxscaffold_6G00430610 Rroxscaffold_7G00162630 Rroxscaffold_7G00162640 Rroxscaffold_7G00164320 Rroxscaffold_7G00214930
rosa_rugosa Rorug01G0012500 Rorug01G0018000 Rorug01G0019000 Rorug01G0019000 Rorug01G0070900 Rorug01G0094400 Rorug02G0043200 Rorug03G0329700 Rorug03G0329800 Rorug03G0329900 Rorug03G0332900 Rorug03G0343500 Rorug03G0343600 Rorug03G0343700 Rorug04G0032000 Rorug04G0065800 Rorug05G0102700 Rorug05G0106200 Rorug05G0256700 Rorug05G0256700 Rorug05G0439000 Rorug05G0540900 Rorug06G0055100 Rorug06G0060400 Rorug06G0170800 Rorug06G0184400 Rorug06G0439700 Rorug07G0182300 Rorug07G0182400 Rorug07G0182500 Rorug07G0208100 Rorug07G0208200 Rorug07G0208200 Rorug07G0283700.1 Rorug07G0283800
rosa_samantha Rh1AG090000 Rh1AG169000 Rh1BG060800 Rh1BG060900 Rh1BG070000 Rh1BG407100 Rh1BG407200 Rh1BG407400 Rh1BG407500 Rh1CG028100 Rh1CG028200 Rh1CG040100 Rh1CG050100 Rh1CG055500 Rh1CG156800 Rh1CG421400 Rh1CG421500 Rh1CG421600 Rh1DG029300 Rh1DG054600 Rh1DG054700 Rh1DG059400 Rh1DG177400 Rh1DG438200 Rh1DG438300 Rh1DG438500 Rh1DG438600 Rh1DG438700 Rh2AG286700 Rh2AG286800 Rh2AG286900 Rh2AG311900 Rh2AG322600 Rh2AG353300 Rh2AG353400 Rh2AG560900 Rh2AG642800 Rh2AG642900 Rh2AG643000 Rh2AG643100 Rh2AG647000 Rh2BG288800 Rh2BG317900 Rh2BG394800 Rh2BG394900 Rh2BG594000 Rh2BG657700 Rh2CG299000 Rh2CG299100 Rh2CG300200 Rh2DG102500 Rh2DG284000 Rh2DG312200 Rh2DG312300 Rh2DG336300 Rh2DG336400 Rh2DG337200 Rh2DG571300 Rh3AG246800 Rh3AG272200 Rh3BG121800 Rh3BG249400 Rh3BG265300 Rh3BG341500 Rh3BG348700 Rh3CG301300 Rh3CG307800 Rh3CG307900 Rh3CG308100 Rh3DG144400 Rh3DG251600 Rh3DG297700 Rh3DG301700 Rh3DG305100 Rh3DG340900 Rh4AG149000 Rh4AG248900 Rh4AG336100 Rh4BG044000 Rh4BG111400 Rh4BG133400 Rh4BG182300 Rh4BG219600 Rh4BG219700 Rh4BG254300 Rh4BG388300 Rh4BG388600 Rh4CG126200 Rh4CG155800 Rh4CG257000 Rh4CG402900 Rh4CG403000 Rh4DG005400 Rh4DG057400 Rh4DG057500 Rh4DG057700 Rh4DG120700 Rh4DG280800 Rh4DG338600 Rh5AG057600 Rh5AG057700 Rh5AG057800 Rh5AG284700 Rh5AG284800 Rh5AG305600 Rh5AG364300 Rh5AG365100 Rh5AG365200 Rh5AG516000 Rh5AG516200 Rh5BG157600 Rh5BG235200 Rh5BG235400 Rh5BG308900 Rh5BG373800 Rh5BG414500 Rh5BG538800 Rh5CG102300 Rh5CG248400 Rh5CG249300 Rh5CG249400 Rh5CG326300 Rh5CG326400 Rh5CG366400 Rh5CG366500 Rh5CG439100 Rh5CG439200 Rh5CG497700 Rh5CG562500 Rh5CG567300 Rh5DG179600 Rh5DG179700 Rh5DG239900 Rh5DG299700 Rh5DG319400 Rh5DG319500 Rh6AG173400 Rh6AG175300 Rh6AG314800 Rh6AG405000 Rh6BG067300 Rh6BG067400 Rh6BG075300 Rh6BG101700 Rh6BG134300 Rh6BG324500 Rh6CG087200 Rh6CG132400 Rh6CG151800 Rh6CG151900 Rh6CG200100 Rh6CG205500 Rh6CG419400 Rh6CG419500 Rh6DG081400 Rh6DG119900 Rh6DG120000 Rh6DG191500 Rh6DG199900 Rh6DG251900 Rh6DG252000 Rh6DG498400 Rh7AG274800 Rh7AG283100 Rh7AG352600 Rh7AG364900 Rh7AG386800 Rh7BG321800 Rh7BG341100 Rh7CG294000 Rh7CG368900 Rh7CG369900 Rh7CG383700 Rh7CG406000 Rh7CG406100 Rh7DG106300 Rh7DG106400 Rh7DG286300 Rh7DG346900 Rh7DG353400 Rh7DG360000 Rh7DG361700 Rh7DG362200 Rh7DG362600 Rh7DG362900 Rh7DG363400
rosa_wichuraiana Rw0G000990 Rw0G001740 Rw0G002300 Rw0G002310 Rw0G007890 Rw0G007900 Rw0G023450 Rw0G023460 Rw1G000300 Rw1G007140 Rw1G007170 Rw1G008970 Rw1G010460 Rw1G039090 Rw1G039100 Rw2G012530 Rw2G016610 Rw2G023010 Rw2G031730 Rw3G014310 Rw4G008550 Rw4G010240 Rw4G012160 Rw4G024150 Rw5G009090 Rw5G047250 Rw5G048500 Rw5G050510 Rw6G006430 Rw6G006440 Rw6G006450 Rw6G013330 Rw6G016560 Rw6G021330 Rw6G024630 Rw6G035440 Rw6G041540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AasI GACNNNNNNGTC 1 cut(s) 1457
AccB7I CCANNNNNTGG 1 cut(s) 1005
AccI GTMKAC 3 cut(s) 256, 1010, 1677
AccII CGCG 2 cut(s) 1770, 2287
AccIII TCCGGA 1 cut(s) 2034
AclWI GGATC 8 cut(s) 695, 708, 722, 735, 1045, 1360, 2089, 2102
AcoI YGGCCR 1 cut(s) 1729
AcsI RAATTY 3 cut(s) 269, 1124, 2264
AcuI CTGAAG 1 cut(s) 1947
AfaI GTAC 6 cut(s) 138, 286, 474, 912, 1054, 1322
AfiI CCNNNNNNNGG 6 cut(s) 251, 958, 1005, 1276, 1347, 1988
AflIII ACRYGT 1 cut(s) 1332
AhdI GACNNNNNGTC 1 cut(s) 1707
AjiI CACGTC 1 cut(s) 2201
AjnI CCWGG 2 cut(s) 704, 951
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
Alw26I GTCTC 4 cut(s) 143, 430, 656, 2149
AlwI GGATC 8 cut(s) 695, 708, 722, 735, 1045, 1360, 2089, 2102
AlwNI CAGNNNCTG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 2034
AoxI GGCC 5 cut(s) 606, 1174, 1370, 1729, 1990
ApaI GGGCCC 1 cut(s) 1994
ApeKI GCWGC 6 cut(s) 847, 1842, 2156, 2159, 2162, 2204
ApoI RAATTY 3 cut(s) 269, 1124, 2264
AspLEI GCGC 1 cut(s) 1770
AspS9I GGNCC 6 cut(s) 607, 1174, 1370, 1600, 1990, 1991
AsuHPI GGTGA 9 cut(s) 206, 250, 442, 1490, 1540, 1608, 1711, 2096, 2114
AvaII GGWCC 1 cut(s) 1600
BaeGI GKGCMC 1 cut(s) 1994
BamHI GGATCC 3 cut(s) 700, 727, 2094
BanII GRGCYC 1 cut(s) 1994
BbsI GAAGAC 1 cut(s) 174
BbvI GCAGC 6 cut(s) 859, 1829, 2168, 2171, 2174, 2216
BccI CCATC 6 cut(s) 127, 209, 482, 595, 1525, 1590
BceAI ACGGC 1 cut(s) 704
BciT130I CCWGG 2 cut(s) 706, 953
BciVI GTATCC 1 cut(s) 856
BcoDI GTCTC 4 cut(s) 143, 430, 656, 2149
BfaI CTAG 3 cut(s) 1085, 1593, 1958
BfmI CTRYAG 4 cut(s) 246, 456, 845, 1971
BfuI GTATCC 1 cut(s) 856
BglII AGATCT 2 cut(s) 1081, 1139
Bme1390I CCNGG 2 cut(s) 706, 953
Bme18I GGWCC 1 cut(s) 1600
BmeRI GACNNNNNGTC 1 cut(s) 1707
BmgBI CACGTC 1 cut(s) 2201
BmgT120I GGNCC 6 cut(s) 607, 1174, 1370, 1600, 1990, 1991
BmiI GGNNCC 5 cut(s) 702, 729, 1601, 1992, 2096
BmrFI CCNGG 2 cut(s) 706, 953
BmrI ACTGGG 2 cut(s) 763, 1335
BmsI GCATC 8 cut(s) 125, 310, 577, 682, 1036, 1099, 1611, 2173
BmuI ACTGGG 2 cut(s) 763, 1335
BoxI GACNNNNGTC 1 cut(s) 1335
BpiI GAAGAC 1 cut(s) 174
BpmI CTGGAG 2 cut(s) 246, 1832
Bpu10I CCTNAGC 2 cut(s) 1650, 2166
BpuEI CTTGAG 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 704
BsaWI WCCGGW 8 cut(s) 235, 529, 907, 1162, 2018, 2034, 2052, 2081
BsaXI ACNNNNNCTCC 2 cut(s) 1271, 1301
Bsc4I CCNNNNNNNGG 6 cut(s) 251, 958, 1005, 1276, 1347, 1988
Bse1I ACTGG 5 cut(s) 229, 292, 758, 988, 1341
Bse3DI GCAATG 1 cut(s) 1189
BseAI TCCGGA 1 cut(s) 2034
BseBI CCWGG 2 cut(s) 706, 953
BseDI CCNNGG 1 cut(s) 704
BseGI GGATG 5 cut(s) 493, 592, 1239, 1312, 2223
BseLI CCNNNNNNNGG 6 cut(s) 251, 958, 1005, 1276, 1347, 1988
BseMI GCAATG 1 cut(s) 1189
BseMII CTCAG 5 cut(s) 6, 467, 1527, 2024, 2180
BseNI ACTGG 5 cut(s) 229, 292, 758, 988, 1341
BseRI GAGGAG 4 cut(s) 32, 314, 1145, 1292
BseSI GKGCMC 1 cut(s) 1994
BseX3I CGGCCG 1 cut(s) 1729
BseXI GCAGC 6 cut(s) 859, 1829, 2168, 2171, 2174, 2216
BsgI GTGCAG 2 cut(s) 1491, 2223
Bsh1236I CGCG 2 cut(s) 1770, 2287
Bsh1285I CGRYCG 1 cut(s) 1732
BshFI GGCC 5 cut(s) 608, 1176, 1372, 1731, 1992
BsiEI CGRYCG 1 cut(s) 1732
BsiSI CCGG 9 cut(s) 236, 530, 908, 1035, 1163, 2019, 2035, 2053, 2082
BslFI GGGAC 4 cut(s) 82, 1323, 1613, 1850
BslI CCNNNNNNNGG 6 cut(s) 251, 958, 1005, 1276, 1347, 1988
BsmAI GTCTC 4 cut(s) 143, 430, 656, 2149
BsmFI GGGAC 4 cut(s) 82, 1323, 1613, 1850
BsmI GAATGC 2 cut(s) 1861, 2137
BsnI GGCC 5 cut(s) 608, 1176, 1372, 1731, 1992
Bsp120I GGGCCC 1 cut(s) 1990
Bsp1286I GDGCHC 1 cut(s) 1994
Bsp13I TCCGGA 1 cut(s) 2034
Bsp143I GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
BspANI GGCC 5 cut(s) 608, 1176, 1372, 1731, 1992
BspCNI CTCAG 5 cut(s) 7, 468, 1528, 2023, 2179
BspEI TCCGGA 1 cut(s) 2034
BspFNI CGCG 2 cut(s) 1770, 2287
BspLI GGNNCC 5 cut(s) 702, 729, 1601, 1992, 2096
BspMAI CTGCAG 2 cut(s) 460, 849
BspPI GGATC 8 cut(s) 695, 708, 722, 735, 1045, 1360, 2089, 2102
BsrDI GCAATG 1 cut(s) 1189
BsrI ACTGG 5 cut(s) 229, 292, 758, 988, 1341
BssECI CCNNGG 1 cut(s) 704
BssMI GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
Bst2UI CCWGG 2 cut(s) 706, 953
Bst4CI ACNGT 4 cut(s) 154, 448, 1547, 1775
BstAPI GCANNNNNTGC 1 cut(s) 1949
BstC8I GCNNGC 4 cut(s) 56, 933, 1555, 1748
BstDEI CTNAG 6 cut(s) 15, 476, 1536, 1650, 2010, 2166
BstEII GGTNACC 1 cut(s) 2084
BstENI CCTNNNNNAGG 2 cut(s) 249, 1274
BstF5I GGATG 5 cut(s) 493, 592, 1239, 1312, 2223
BstFNI CGCG 2 cut(s) 1770, 2287
BstHHI GCGC 1 cut(s) 1770
BstKTI GATC 8 cut(s) 703, 730, 1040, 1084, 1142, 1355, 1582, 2097
BstMAI GTCTC 4 cut(s) 143, 430, 656, 2149
BstMBI GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
BstMCI CGRYCG 1 cut(s) 1732
BstMWI GCNNNNNNNGC 7 cut(s) 86, 320, 764, 1354, 1949, 1958, 2162
BstNI CCWGG 2 cut(s) 706, 953
BstNSI RCATGY 3 cut(s) 58, 1336, 2044
BstPAI GACNNNNGTC 1 cut(s) 1335
BstPI GGTNACC 1 cut(s) 2084
BstSCI CCNGG 2 cut(s) 704, 951
BstSFI CTRYAG 4 cut(s) 246, 456, 845, 1971
BstSLI GKGCMC 1 cut(s) 1994
BstUI CGCG 2 cut(s) 1770, 2287
BstV1I GCAGC 6 cut(s) 859, 1829, 2168, 2171, 2174, 2216
BstV2I GAAGAC 1 cut(s) 174
BstX2I RGATCY 6 cut(s) 700, 727, 1037, 1081, 1139, 2094
BstYI RGATCY 6 cut(s) 700, 727, 1037, 1081, 1139, 2094
BstZI CGGCCG 1 cut(s) 1729
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 5 cut(s) 608, 1176, 1372, 1731, 1992
BtrI CACGTC 1 cut(s) 2201
BtsCI GGATG 5 cut(s) 493, 592, 1239, 1312, 2223
BtsI GCAGTG 3 cut(s) 702, 1196, 2142
BtsIMutI CAGTG 4 cut(s) 702, 1196, 1971, 2142
Cac8I GCNNGC 4 cut(s) 56, 933, 1555, 1748
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 1770
Cfr13I GGNCC 6 cut(s) 607, 1174, 1370, 1600, 1990, 1991
CseI GACGC 2 cut(s) 267, 1856
Csp6I GTAC 6 cut(s) 137, 285, 473, 911, 1053, 1321
CspCI CAANNNNNGTGG 2 cut(s) 541, 576
CviQI GTAC 6 cut(s) 137, 285, 473, 911, 1053, 1321
DdeI CTNAG 6 cut(s) 15, 476, 1536, 1650, 2010, 2166
DpnI GATC 8 cut(s) 702, 729, 1039, 1083, 1141, 1354, 1581, 2096
DpnII GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
DrdI GACNNNNNNGTC 1 cut(s) 1457
DriI GACNNNNNGTC 1 cut(s) 1707
DseDI GACNNNNNNGTC 1 cut(s) 1457
EaeI YGGCCR 1 cut(s) 1729
EagI CGGCCG 1 cut(s) 1729
Eam1105I GACNNNNNGTC 1 cut(s) 1707
EciI GGCGGA 2 cut(s) 455, 2087
EclXI CGGCCG 1 cut(s) 1729
Eco24I GRGCYC 1 cut(s) 1994
Eco32I GATATC 1 cut(s) 1689
Eco47I GGWCC 1 cut(s) 1600
Eco52I CGGCCG 1 cut(s) 1729
Eco57I CTGAAG 1 cut(s) 1947
Eco91I GGTNACC 1 cut(s) 2084
EcoNI CCTNNNNNAGG 2 cut(s) 249, 1274
EcoO109I RGGNCCY 2 cut(s) 1600, 1990
EcoO65I GGTNACC 1 cut(s) 2084
EcoRII CCWGG 2 cut(s) 704, 951
EcoRV GATATC 1 cut(s) 1689
EcoT38I GRGCYC 1 cut(s) 1994
FalI AAGNNNNNCTT 2 cut(s) 1945, 1977
FaqI GGGAC 4 cut(s) 82, 1323, 1613, 1850
FauI CCCGC 2 cut(s) 1341, 1948
FblI GTMKAC 3 cut(s) 256, 1010, 1677
FokI GGATG 5 cut(s) 500, 599, 1246, 1319, 2210
FriOI GRGCYC 1 cut(s) 1994
FspBI CTAG 3 cut(s) 1085, 1593, 1958
GlaI GCGC 1 cut(s) 1769
GsuI CTGGAG 2 cut(s) 246, 1832
HaeIII GGCC 5 cut(s) 608, 1176, 1372, 1731, 1992
HapII CCGG 9 cut(s) 236, 530, 908, 1035, 1163, 2019, 2035, 2053, 2082
HgaI GACGC 2 cut(s) 267, 1856
HhaI GCGC 1 cut(s) 1770
Hin6I GCGC 1 cut(s) 1768
HinP1I GCGC 1 cut(s) 1768
HincII GTYRAC 3 cut(s) 257, 666, 1011
HindII GTYRAC 3 cut(s) 257, 666, 1011
HindIII AAGCTT 4 cut(s) 517, 580, 776, 1408
HpaII CCGG 9 cut(s) 236, 530, 908, 1035, 1163, 2019, 2035, 2053, 2082
HphI GGTGA 9 cut(s) 206, 250, 442, 1490, 1540, 1608, 1711, 2096, 2114
Hpy166II GTNNAC 4 cut(s) 257, 666, 1011, 1678
Hpy188III TCNNGA 9 cut(s) 424, 1085, 1096, 1593, 1811, 1871, 2012, 2035, 2168
Hpy8I GTNNAC 4 cut(s) 257, 666, 1011, 1678
Hpy99I CGWCG 6 cut(s) 13, 127, 261, 1775, 1872, 2280
HpyAV CCTTC 6 cut(s) 779, 789, 1255, 1291, 1724, 2063
HpyCH4III ACNGT 4 cut(s) 154, 448, 1547, 1775
HpyCH4IV ACGT 5 cut(s) 347, 369, 501, 1645, 2200
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 320, 764, 1354, 1949, 1958, 2162
HpyF3I CTNAG 6 cut(s) 15, 476, 1536, 1650, 2010, 2166
HpySE526I ACGT 5 cut(s) 347, 369, 501, 1645, 2200
HspAI GCGC 1 cut(s) 1768
Kpn2I TCCGGA 1 cut(s) 2034
Kzo9I GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
LmnI GCTCC 2 cut(s) 31, 1897
Lsp1109I GCAGC 6 cut(s) 859, 1829, 2168, 2171, 2174, 2216
LweI GCATC 8 cut(s) 125, 310, 577, 682, 1036, 1099, 1611, 2173
MaeI CTAG 3 cut(s) 1085, 1593, 1958
MaeII ACGT 5 cut(s) 347, 369, 501, 1645, 2200
MaeIII GTNAC 6 cut(s) 44, 448, 1238, 1614, 1699, 2084
MalI GATC 8 cut(s) 702, 729, 1039, 1083, 1141, 1354, 1581, 2096
MboI GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
MboII GAAGA 6 cut(s) 179, 610, 704, 1091, 1337, 1750
MfeI CAATTG 2 cut(s) 1435, 2178
MflI RGATCY 6 cut(s) 700, 727, 1037, 1081, 1139, 2094
MhlI GDGCHC 1 cut(s) 1994
MlyI GAGTC 8 cut(s) 64, 164, 250, 971, 1505, 1547, 1883, 2002
MmeI TCCRAC 5 cut(s) 105, 899, 917, 1512, 2258
MroI TCCGGA 1 cut(s) 2034
MseI TTAA 4 cut(s) 366, 443, 509, 1398
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 4 cut(s) 51, 850, 1348, 1842
MspI CCGG 9 cut(s) 236, 530, 908, 1035, 1163, 2019, 2035, 2053, 2082
MspR9I CCNGG 2 cut(s) 706, 953
MunI CAATTG 2 cut(s) 1435, 2178
Mva1269I GAATGC 2 cut(s) 1861, 2137
MvaI CCWGG 2 cut(s) 706, 953
MvnI CGCG 2 cut(s) 1770, 2287
MwoI GCNNNNNNNGC 7 cut(s) 86, 320, 764, 1354, 1949, 1958, 2162
NdeII GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
NlaIV GGNNCC 5 cut(s) 702, 729, 1601, 1992, 2096
NmeAIII GCCGAG 2 cut(s) 705, 1383
NmuCI GTSAC 5 cut(s) 44, 448, 1614, 1699, 2084
NspI RCATGY 3 cut(s) 58, 1336, 2044
PaeI GCATGC 1 cut(s) 58
PciI ACATGT 1 cut(s) 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PctI GAATGC 2 cut(s) 1861, 2137
PfeI GAWTC 6 cut(s) 427, 1020, 1310, 1807, 2015, 2119
PflFI GACNNNGTC 2 cut(s) 1091, 1866
PflMI CCANNNNNTGG 1 cut(s) 1005
PleI GAGTC 8 cut(s) 64, 164, 249, 971, 1504, 1546, 1882, 2002
PpsI GAGTC 8 cut(s) 64, 164, 249, 971, 1504, 1546, 1882, 2002
PpuMI RGGWCCY 1 cut(s) 1600
PscI ACATGT 1 cut(s) 1332
PshAI GACNNNNGTC 1 cut(s) 1335
PsiI TTATAA 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 1600
Psp6I CCWGG 2 cut(s) 704, 951
PspEI GGTNACC 1 cut(s) 2084
PspGI CCWGG 2 cut(s) 704, 951
PspN4I GGNNCC 5 cut(s) 702, 729, 1601, 1992, 2096
PspOMI GGGCCC 1 cut(s) 1990
PspPI GGNCC 6 cut(s) 607, 1174, 1370, 1600, 1990, 1991
PspPPI RGGWCCY 1 cut(s) 1600
PstI CTGCAG 2 cut(s) 460, 849
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 6 cut(s) 700, 727, 1037, 1081, 1139, 2094
PsyI GACNNNGTC 2 cut(s) 1091, 1866
PvuII CAGCTG 2 cut(s) 850, 1842
RsaI GTAC 6 cut(s) 138, 286, 474, 912, 1054, 1322
RsaNI GTAC 6 cut(s) 137, 285, 473, 911, 1053, 1321
RseI CAYNNNNRTG 1 cut(s) 186
SalI GTCGAC 2 cut(s) 255, 1009
SaqAI TTAA 4 cut(s) 366, 443, 509, 1398
Sau3AI GATC 8 cut(s) 700, 727, 1037, 1081, 1139, 1352, 1579, 2094
Sau96I GGNCC 6 cut(s) 607, 1174, 1370, 1600, 1990, 1991
SchI GAGTC 8 cut(s) 64, 164, 250, 971, 1505, 1547, 1883, 2002
ScrFI CCNGG 2 cut(s) 706, 953
SduI GDGCHC 1 cut(s) 1994
SfaNI GCATC 8 cut(s) 125, 310, 577, 682, 1036, 1099, 1611, 2173
SfcI CTRYAG 4 cut(s) 246, 456, 845, 1971
SinI GGWCC 1 cut(s) 1600
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 72
SmoI CTYRAG 1 cut(s) 72
SphI GCATGC 1 cut(s) 58
SspI AATATT 1 cut(s) 2239
SspMI CTAG 3 cut(s) 1085, 1593, 1958
StyD4I CCNGG 2 cut(s) 704, 951
TaaI ACNGT 4 cut(s) 154, 448, 1547, 1775
TaiI ACGT 5 cut(s) 350, 372, 504, 1648, 2203
TaqI TCGA 5 cut(s) 8, 256, 980, 1010, 1018
TatI WGTACW 3 cut(s) 284, 472, 1052
TauI GCSGC 5 cut(s) 54, 83, 1375, 1731, 2290
TfiI GAWTC 6 cut(s) 427, 1020, 1310, 1807, 2015, 2119
Tru1I TTAA 4 cut(s) 366, 443, 509, 1398
Tru9I TTAA 4 cut(s) 366, 443, 509, 1398
TscAI CASTG 4 cut(s) 702, 1203, 1971, 2142
TseFI GTSAC 5 cut(s) 44, 448, 1614, 1699, 2084
TseI GCWGC 6 cut(s) 847, 1842, 2156, 2159, 2162, 2204
Tsp45I GTSAC 5 cut(s) 44, 448, 1614, 1699, 2084
TspDTI ATGAA 8 cut(s) 890, 1274, 1323, 1868, 1938, 2144, 2212, 2276
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 4 cut(s) 702, 1203, 1971, 2142
Tth111I GACNNNGTC 2 cut(s) 1091, 1866
Van91I CCANNNNNTGG 1 cut(s) 1005
VpaK11BI GGWCC 1 cut(s) 1600
XagI CCTNNNNNAGG 2 cut(s) 249, 1274
XapI RAATTY 3 cut(s) 269, 1124, 2264
XbaI TCTAGA 2 cut(s) 1084, 1592
XceI RCATGY 3 cut(s) 58, 1336, 2044
XmiI GTMKAC 3 cut(s) 256, 1010, 1677
XspI CTAG 3 cut(s) 1085, 1593, 1958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.