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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 818 |
| AciI | CCGC | 2 cut(s) | 241, 994 |
| AclWI | GGATC | 2 cut(s) | 152, 725 |
| AcoI | YGGCCR | 2 cut(s) | 430, 588 |
| AcsI | RAATTY | 4 cut(s) | 56, 525, 695, 925 |
| AcuI | CTGAAG | 2 cut(s) | 213, 380 |
| AfaI | GTAC | 3 cut(s) | 160, 405, 845 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 392, 853, 891 |
| AgsI | TTSAA | 7 cut(s) | 170, 234, 293, 616, 672, 694, 991 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 865 |
| AjnI | CCWGG | 1 cut(s) | 734 |
| AleI | CACNNNNGTG | 1 cut(s) | 960 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 214, 246 |
| AluBI | AGCT | 5 cut(s) | 200, 226, 500, 539, 1011 |
| AluI | AGCT | 5 cut(s) | 200, 226, 500, 539, 1011 |
| Alw21I | GWGCWC | 1 cut(s) | 1001 |
| Alw26I | GTCTC | 3 cut(s) | 113, 257, 708 |
| AlwI | GGATC | 2 cut(s) | 152, 725 |
| AoxI | GGCC | 3 cut(s) | 430, 508, 588 |
| ApeKI | GCWGC | 1 cut(s) | 839 |
| ApoI | RAATTY | 4 cut(s) | 56, 525, 695, 925 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 355, 387 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 667 |
| AspLEI | GCGC | 1 cut(s) | 819 |
| AsuHPI | GGTGA | 3 cut(s) | 196, 589, 791 |
| AsuII | TTCGAA | 1 cut(s) | 374 |
| AxyI | CCTNAGG | 1 cut(s) | 387 |
| BalI | TGGCCA | 1 cut(s) | 432 |
| BanII | GRGCYC | 1 cut(s) | 255 |
| BauI | CACGAG | 1 cut(s) | 545 |
| BbsI | GAAGAC | 1 cut(s) | 368 |
| Bbv12I | GWGCWC | 1 cut(s) | 1001 |
| BbvI | GCAGC | 1 cut(s) | 826 |
| BccI | CCATC | 5 cut(s) | 26, 131, 422, 500, 601 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 364, 398 |
| BciT130I | CCWGG | 1 cut(s) | 736 |
| BclI | TGATCA | 1 cut(s) | 556 |
| BcoDI | GTCTC | 3 cut(s) | 113, 257, 708 |
| BfaI | CTAG | 1 cut(s) | 407 |
| BfmI | CTRYAG | 3 cut(s) | 501, 515, 968 |
| BisI | GCNGC | 1 cut(s) | 840 |
| BlsI | GCNGC | 1 cut(s) | 841 |
| Bme1390I | CCNGG | 1 cut(s) | 736 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 865 |
| BmiI | GGNNCC | 1 cut(s) | 252 |
| BmrFI | CCNGG | 1 cut(s) | 736 |
| BpiI | GAAGAC | 1 cut(s) | 368 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 845, 877 |
| Bpu14I | TTCGAA | 1 cut(s) | 374 |
| BsaJI | CCNNGG | 3 cut(s) | 414, 734, 735 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 31 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 392, 853, 891 |
| Bse21I | CCTNAGG | 1 cut(s) | 387 |
| Bse3DI | GCAATG | 1 cut(s) | 794 |
| BseBI | CCWGG | 1 cut(s) | 736 |
| BseDI | CCNNGG | 3 cut(s) | 414, 734, 735 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 392, 853, 891 |
| BseMI | GCAATG | 1 cut(s) | 794 |
| BseMII | CTCAG | 1 cut(s) | 401 |
| BseRI | GAGGAG | 1 cut(s) | 869 |
| BseXI | GCAGC | 1 cut(s) | 826 |
| BshFI | GGCC | 3 cut(s) | 432, 510, 590 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1001 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 392, 853, 891 |
| BsmAI | GTCTC | 3 cut(s) | 113, 257, 708 |
| BsnI | GGCC | 3 cut(s) | 432, 510, 590 |
| Bsp119I | TTCGAA | 1 cut(s) | 374 |
| Bsp1286I | GDGCHC | 2 cut(s) | 255, 1001 |
| Bsp1407I | TGTACA | 1 cut(s) | 158 |
| Bsp143I | GATC | 4 cut(s) | 77, 144, 556, 730 |
| BspACI | CCGC | 2 cut(s) | 241, 994 |
| BspANI | GGCC | 3 cut(s) | 432, 510, 590 |
| BspCNI | CTCAG | 1 cut(s) | 400 |
| BspLI | GGNNCC | 1 cut(s) | 252 |
| BspMAI | CTGCAG | 1 cut(s) | 519 |
| BspPI | GGATC | 2 cut(s) | 152, 725 |
| BspT104I | TTCGAA | 1 cut(s) | 374 |
| BsrDI | GCAATG | 1 cut(s) | 794 |
| BsrGI | TGTACA | 1 cut(s) | 158 |
| BssECI | CCNNGG | 3 cut(s) | 414, 734, 735 |
| BssMI | GATC | 4 cut(s) | 77, 144, 556, 730 |
| BssSI | CACGAG | 1 cut(s) | 545 |
| BssT1I | CCWWGG | 1 cut(s) | 414 |
| Bst2BI | CACGAG | 1 cut(s) | 545 |
| Bst2UI | CCWGG | 1 cut(s) | 736 |
| Bst4CI | ACNGT | 5 cut(s) | 41, 99, 158, 297, 382 |
| Bst6I | CTCTTC | 1 cut(s) | 41 |
| BstAUI | TGTACA | 1 cut(s) | 158 |
| BstBI | TTCGAA | 1 cut(s) | 374 |
| BstC8I | GCNNGC | 1 cut(s) | 1009 |
| BstDEI | CTNAG | 4 cut(s) | 387, 456, 794, 1002 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 390, 851 |
| BstHHI | GCGC | 1 cut(s) | 819 |
| BstKTI | GATC | 4 cut(s) | 80, 147, 559, 733 |
| BstMAI | GTCTC | 3 cut(s) | 113, 257, 708 |
| BstMBI | GATC | 4 cut(s) | 77, 144, 556, 730 |
| BstNI | CCWGG | 1 cut(s) | 736 |
| BstNSI | RCATGY | 1 cut(s) | 935 |
| BstSCI | CCNGG | 1 cut(s) | 734 |
| BstSFI | CTRYAG | 3 cut(s) | 501, 515, 968 |
| BstV1I | GCAGC | 1 cut(s) | 826 |
| BstV2I | GAAGAC | 1 cut(s) | 368 |
| Bsu36I | CCTNAGG | 1 cut(s) | 387 |
| BsuRI | GGCC | 3 cut(s) | 432, 510, 590 |
| BtsIMutI | CAGTG | 3 cut(s) | 154, 161, 378 |
| Cac8I | GCNNGC | 1 cut(s) | 1009 |
| CfoI | GCGC | 1 cut(s) | 819 |
| Csp6I | GTAC | 3 cut(s) | 159, 404, 844 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 871, 906 |
| CviAII | CATG | 5 cut(s) | 259, 486, 870, 914, 932 |
| CviQI | GTAC | 3 cut(s) | 159, 404, 844 |
| DdeI | CTNAG | 4 cut(s) | 387, 456, 794, 1002 |
| DpnI | GATC | 4 cut(s) | 79, 146, 558, 732 |
| DpnII | GATC | 4 cut(s) | 77, 144, 556, 730 |
| DriI | GACNNNNNGTC | 1 cut(s) | 865 |
| EaeI | YGGCCR | 2 cut(s) | 430, 588 |
| Eam1104I | CTCTTC | 1 cut(s) | 41 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 865 |
| EarI | CTCTTC | 1 cut(s) | 41 |
| Eco130I | CCWWGG | 1 cut(s) | 414 |
| Eco24I | GRGCYC | 1 cut(s) | 255 |
| Eco57I | CTGAAG | 2 cut(s) | 213, 380 |
| Eco81I | CCTNAGG | 1 cut(s) | 387 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 390, 851 |
| EcoRI | GAATTC | 1 cut(s) | 925 |
| EcoRII | CCWGG | 1 cut(s) | 734 |
| EcoT14I | CCWWGG | 1 cut(s) | 414 |
| EcoT22I | ATGCAT | 1 cut(s) | 937 |
| EcoT38I | GRGCYC | 1 cut(s) | 255 |
| ErhI | CCWWGG | 1 cut(s) | 414 |
| FaeI | CATG | 5 cut(s) | 262, 489, 873, 917, 935 |
| FatI | CATG | 5 cut(s) | 258, 485, 869, 913, 931 |
| FauNDI | CATATG | 1 cut(s) | 561 |
| FbaI | TGATCA | 1 cut(s) | 556 |
| Fnu4HI | GCNGC | 1 cut(s) | 840 |
| FriOI | GRGCYC | 1 cut(s) | 255 |
| Fsp4HI | GCNGC | 1 cut(s) | 840 |
| FspBI | CTAG | 1 cut(s) | 407 |
| FspI | TGCGCA | 1 cut(s) | 818 |
| GlaI | GCGC | 1 cut(s) | 818 |
| GluI | GCNGC | 1 cut(s) | 840 |
| HaeIII | GGCC | 3 cut(s) | 432, 510, 590 |
| HhaI | GCGC | 1 cut(s) | 819 |
| Hin1II | CATG | 5 cut(s) | 262, 489, 873, 917, 935 |
| Hin6I | GCGC | 1 cut(s) | 817 |
| HinP1I | GCGC | 1 cut(s) | 817 |
| HincII | GTYRAC | 1 cut(s) | 948 |
| HindII | GTYRAC | 1 cut(s) | 948 |
| HindIII | AAGCTT | 1 cut(s) | 224 |
| HinfI | GANTC | 5 cut(s) | 329, 410, 620, 680, 860 |
| HphI | GGTGA | 3 cut(s) | 196, 589, 791 |
| Hpy166II | GTNNAC | 3 cut(s) | 161, 444, 948 |
| Hpy188I | TCNGA | 6 cut(s) | 90, 149, 193, 307, 360, 532 |
| Hpy8I | GTNNAC | 3 cut(s) | 161, 444, 948 |
| HpyAV | CCTTC | 7 cut(s) | 164, 307, 521, 544, 568, 619, 670 |
| HpyCH4III | ACNGT | 5 cut(s) | 41, 99, 158, 297, 382 |
| HpyCH4V | TGCA | 5 cut(s) | 154, 489, 517, 787, 935 |
| HpyF3I | CTNAG | 4 cut(s) | 387, 456, 794, 1002 |
| Hsp92II | CATG | 5 cut(s) | 262, 489, 873, 917, 935 |
| HspAI | GCGC | 1 cut(s) | 817 |
| Ksp22I | TGATCA | 1 cut(s) | 556 |
| Kzo9I | GATC | 4 cut(s) | 77, 144, 556, 730 |
| LmnI | GCTCC | 3 cut(s) | 9, 250, 544 |
| LpnPI | CCDG | 6 cut(s) | 167, 374, 721, 748, 860, 898 |
| Lsp1109I | GCAGC | 1 cut(s) | 826 |
| MaeI | CTAG | 1 cut(s) | 407 |
| MaeIII | GTNAC | 1 cut(s) | 577 |
| MalI | GATC | 4 cut(s) | 79, 146, 558, 732 |
| MboI | GATC | 4 cut(s) | 77, 144, 556, 730 |
| MboII | GAAGA | 5 cut(s) | 58, 63, 373, 752, 868 |
| MhlI | GDGCHC | 2 cut(s) | 255, 1001 |
| MlsI | TGGCCA | 1 cut(s) | 432 |
| MluCI | AATT | 9 cut(s) | 56, 234, 288, 436, 525, 584, 695, 782, 925 |
| MluNI | TGGCCA | 1 cut(s) | 432 |
| MlyI | GAGTC | 3 cut(s) | 614, 674, 854 |
| MnlI | CCTC | 5 cut(s) | 396, 412, 847, 850, 966 |
| Mox20I | TGGCCA | 1 cut(s) | 432 |
| Mph1103I | ATGCAT | 1 cut(s) | 937 |
| MroXI | GAANNNNTTC | 1 cut(s) | 667 |
| MscI | TGGCCA | 1 cut(s) | 432 |
| MseI | TTAA | 2 cut(s) | 351, 630 |
| MslI | CAYNNNNRTG | 1 cut(s) | 960 |
| Msp20I | TGGCCA | 1 cut(s) | 432 |
| MspR9I | CCNGG | 1 cut(s) | 736 |
| MvaI | CCWGG | 1 cut(s) | 736 |
| NdeI | CATATG | 1 cut(s) | 561 |
| NdeII | GATC | 4 cut(s) | 77, 144, 556, 730 |
| NlaIII | CATG | 5 cut(s) | 262, 489, 873, 917, 935 |
| NlaIV | GGNNCC | 1 cut(s) | 252 |
| NmuCI | GTSAC | 1 cut(s) | 577 |
| NsbI | TGCGCA | 1 cut(s) | 818 |
| NsiI | ATGCAT | 1 cut(s) | 937 |
| NspI | RCATGY | 1 cut(s) | 935 |
| NspV | TTCGAA | 1 cut(s) | 374 |
| OliI | CACNNNNGTG | 1 cut(s) | 960 |
| PasI | CCCWGGG | 1 cut(s) | 735 |
| PdmI | GAANNNNTTC | 1 cut(s) | 667 |
| PfeI | GAWTC | 2 cut(s) | 329, 410 |
| PkrI | GCNGC | 1 cut(s) | 841 |
| PleI | GAGTC | 3 cut(s) | 614, 674, 854 |
| PpsI | GAGTC | 3 cut(s) | 614, 674, 854 |
| Psp6I | CCWGG | 1 cut(s) | 734 |
| PspGI | CCWGG | 1 cut(s) | 734 |
| PspN4I | GGNNCC | 1 cut(s) | 252 |
| PstI | CTGCAG | 1 cut(s) | 519 |
| RsaI | GTAC | 3 cut(s) | 160, 405, 845 |
| RsaNI | GTAC | 3 cut(s) | 159, 404, 844 |
| RseI | CAYNNNNRTG | 1 cut(s) | 960 |
| SaqAI | TTAA | 2 cut(s) | 351, 630 |
| SatI | GCNGC | 1 cut(s) | 840 |
| Sau3AI | GATC | 4 cut(s) | 77, 144, 556, 730 |
| SchI | GAGTC | 3 cut(s) | 614, 674, 854 |
| ScrFI | CCNGG | 1 cut(s) | 736 |
| SduI | GDGCHC | 2 cut(s) | 255, 1001 |
| SfcI | CTRYAG | 3 cut(s) | 501, 515, 968 |
| SfuI | TTCGAA | 1 cut(s) | 374 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 960 |
| Sse9I | AATT | 9 cut(s) | 56, 234, 288, 436, 525, 584, 695, 782, 925 |
| SsiI | CCGC | 2 cut(s) | 241, 994 |
| SspMI | CTAG | 1 cut(s) | 407 |
| StyD4I | CCNGG | 1 cut(s) | 734 |
| StyI | CCWWGG | 1 cut(s) | 414 |
| TaaI | ACNGT | 5 cut(s) | 41, 99, 158, 297, 382 |
| TaqI | TCGA | 3 cut(s) | 374, 623, 923 |
| TasI | AATT | 9 cut(s) | 56, 234, 288, 436, 525, 584, 695, 782, 925 |
| TatI | WGTACW | 1 cut(s) | 158 |
| TfiI | GAWTC | 2 cut(s) | 329, 410 |
| Tru1I | TTAA | 2 cut(s) | 351, 630 |
| Tru9I | TTAA | 2 cut(s) | 351, 630 |
| TscAI | CASTG | 3 cut(s) | 161, 168, 385 |
| TseFI | GTSAC | 1 cut(s) | 577 |
| TseI | GCWGC | 1 cut(s) | 839 |
| Tsp45I | GTSAC | 1 cut(s) | 577 |
| TspDTI | ATGAA | 4 cut(s) | 326, 536, 909, 968 |
| TspGWI | ACGGA | 2 cut(s) | 642, 735 |
| TspRI | CASTG | 3 cut(s) | 161, 168, 385 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 390, 851 |
| XapI | RAATTY | 4 cut(s) | 56, 525, 695, 925 |
| XceI | RCATGY | 1 cut(s) | 935 |
| XmnI | GAANNNNTTC | 1 cut(s) | 667 |
| XspI | CTAG | 1 cut(s) | 407 |
| Zsp2I | ATGCAT | 1 cut(s) | 937 |