MLO-like protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1039 |
| AccI | GTMKAC | 1 cut(s) | 6 |
| AciI | CCGC | 5 cut(s) | 318, 984, 1153, 1297, 1451 |
| AclWI | GGATC | 4 cut(s) | 506, 854, 867, 1208 |
| AcoI | YGGCCR | 4 cut(s) | 945, 1486, 1534, 1692 |
| AcsI | RAATTY | 1 cut(s) | 1629 |
| AcuI | CTGAAG | 1 cut(s) | 372 |
| AfaI | GTAC | 7 cut(s) | 34, 228, 387, 980, 1216, 1461, 1509 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 154, 605, 943 |
| AflIII | ACRYGT | 1 cut(s) | 834 |
| AhlI | ACTAGT | 1 cut(s) | 302 |
| AjiI | CACGTC | 1 cut(s) | 680 |
| AjnI | CCWGG | 1 cut(s) | 936 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1270, 1302 |
| AluBI | AGCT | 8 cut(s) | 332, 718, 814, 923, 1194, 1256, 1282, 1643 |
| AluI | AGCT | 8 cut(s) | 332, 718, 814, 923, 1194, 1256, 1282, 1643 |
| Alw26I | GTCTC | 4 cut(s) | 253, 313, 473, 1313 |
| AlwI | GGATC | 4 cut(s) | 506, 854, 867, 1208 |
| AoxI | GGCC | 5 cut(s) | 596, 945, 1486, 1534, 1692 |
| ApeKI | GCWGC | 4 cut(s) | 814, 1018, 1089, 1191 |
| ApoI | RAATTY | 1 cut(s) | 1629 |
| AseI | ATTAAT | 1 cut(s) | 449 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 741 |
| AspS9I | GGNCC | 1 cut(s) | 1173 |
| AsuHPI | GGTGA | 2 cut(s) | 349, 1693 |
| AvaII | GGWCC | 1 cut(s) | 1173 |
| AxyI | CCTNAGG | 1 cut(s) | 1443 |
| BalI | TGGCCA | 2 cut(s) | 1488, 1536 |
| BamHI | GGATCC | 1 cut(s) | 859 |
| BanI | GGYRCC | 1 cut(s) | 1039 |
| BanII | GRGCYC | 1 cut(s) | 1311 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 192, 224 |
| BbsI | GAAGAC | 1 cut(s) | 95 |
| BbvI | GCAGC | 4 cut(s) | 801, 1030, 1101, 1203 |
| BccI | CCATC | 1 cut(s) | 1526 |
| BceAI | ACGGC | 1 cut(s) | 997 |
| BciT130I | CCWGG | 1 cut(s) | 938 |
| BciVI | GTATCC | 1 cut(s) | 1141 |
| BclI | TGATCA | 2 cut(s) | 535, 1660 |
| BcoDI | GTCTC | 4 cut(s) | 253, 313, 473, 1313 |
| BcuI | ACTAGT | 1 cut(s) | 302 |
| BfaI | CTAG | 5 cut(s) | 303, 797, 1137, 1463, 1511 |
| BfmI | CTRYAG | 2 cut(s) | 1087, 1619 |
| BfuI | GTATCC | 1 cut(s) | 1141 |
| BisI | GCNGC | 5 cut(s) | 318, 815, 1019, 1090, 1192 |
| BlsI | GCNGC | 5 cut(s) | 319, 816, 1020, 1091, 1193 |
| Bme1390I | CCNGG | 1 cut(s) | 938 |
| Bme18I | GGWCC | 1 cut(s) | 1173 |
| BmgBI | CACGTC | 1 cut(s) | 680 |
| BmgT120I | GGNCC | 1 cut(s) | 1173 |
| BmiI | GGNNCC | 5 cut(s) | 861, 1041, 1084, 1175, 1308 |
| BmrFI | CCNGG | 1 cut(s) | 938 |
| BmrI | ACTGGG | 3 cut(s) | 200, 443, 1172 |
| BmsI | GCATC | 1 cut(s) | 174 |
| BmuI | ACTGGG | 3 cut(s) | 200, 443, 1172 |
| BoxI | GACNNNNGTC | 1 cut(s) | 99 |
| BpiI | GAAGAC | 1 cut(s) | 95 |
| BpmI | CTGGAG | 1 cut(s) | 592 |
| BpuEI | CTTGAG | 2 cut(s) | 237, 1216 |
| Bsa29I | ATCGAT | 2 cut(s) | 994, 1050 |
| BsaJI | CCNNGG | 4 cut(s) | 291, 515, 1518, 1544 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 154, 605, 943 |
| Bse118I | RCCGGY | 1 cut(s) | 1059 |
| Bse1I | ACTGG | 4 cut(s) | 195, 438, 770, 1178 |
| Bse21I | CCTNAGG | 1 cut(s) | 1443 |
| BseBI | CCWGG | 1 cut(s) | 938 |
| BseCI | ATCGAT | 2 cut(s) | 994, 1050 |
| BseDI | CCNNGG | 4 cut(s) | 291, 515, 1518, 1544 |
| BseGI | GGATG | 1 cut(s) | 481 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 154, 605, 943 |
| BseMII | CTCAG | 2 cut(s) | 960, 1457 |
| BseNI | ACTGG | 4 cut(s) | 195, 438, 770, 1178 |
| BseX3I | CGGCCG | 1 cut(s) | 1692 |
| BseXI | GCAGC | 4 cut(s) | 801, 1030, 1101, 1203 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1695 |
| BshFI | GGCC | 5 cut(s) | 598, 947, 1488, 1536, 1694 |
| BshNI | GGYRCC | 1 cut(s) | 1039 |
| BshVI | ATCGAT | 2 cut(s) | 994, 1050 |
| BsiEI | CGRYCG | 1 cut(s) | 1695 |
| BsiSI | CCGG | 1 cut(s) | 1060 |
| BslFI | GGGAC | 1 cut(s) | 1159 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 154, 605, 943 |
| BsmAI | GTCTC | 4 cut(s) | 253, 313, 473, 1313 |
| BsmFI | GGGAC | 1 cut(s) | 1159 |
| BsmI | GAATGC | 1 cut(s) | 927 |
| BsnI | GGCC | 5 cut(s) | 598, 947, 1488, 1536, 1694 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1311 |
| Bsp1407I | TGTACA | 1 cut(s) | 1214 |
| Bsp143I | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| Bsp19I | CCATGG | 1 cut(s) | 1544 |
| BspACI | CCGC | 5 cut(s) | 318, 984, 1153, 1297, 1451 |
| BspANI | GGCC | 5 cut(s) | 598, 947, 1488, 1536, 1694 |
| BspCNI | CTCAG | 2 cut(s) | 961, 1456 |
| BspDI | ATCGAT | 2 cut(s) | 994, 1050 |
| BspLI | GGNNCC | 5 cut(s) | 861, 1041, 1084, 1175, 1308 |
| BspMAI | CTGCAG | 2 cut(s) | 1091, 1623 |
| BspPI | GGATC | 4 cut(s) | 506, 854, 867, 1208 |
| BspT107I | GGYRCC | 1 cut(s) | 1039 |
| BsrFI | RCCGGY | 1 cut(s) | 1059 |
| BsrGI | TGTACA | 1 cut(s) | 1214 |
| BsrI | ACTGG | 4 cut(s) | 195, 438, 770, 1178 |
| BssAI | RCCGGY | 1 cut(s) | 1059 |
| BssECI | CCNNGG | 4 cut(s) | 291, 515, 1518, 1544 |
| BssMI | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| BssT1I | CCWWGG | 3 cut(s) | 515, 1518, 1544 |
| Bst2UI | CCWGG | 1 cut(s) | 938 |
| Bst4CI | ACNGT | 2 cut(s) | 1214, 1353 |
| Bst6I | CTCTTC | 1 cut(s) | 591 |
| BstAUI | TGTACA | 1 cut(s) | 1214 |
| BstC8I | GCNNGC | 3 cut(s) | 925, 1061, 1145 |
| BstDEI | CTNAG | 2 cut(s) | 969, 1443 |
| BstDSI | CCRYGG | 1 cut(s) | 1544 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 152 |
| BstF5I | GGATG | 1 cut(s) | 481 |
| BstKTI | GATC | 6 cut(s) | 514, 538, 862, 1050, 1203, 1663 |
| BstMAI | GTCTC | 4 cut(s) | 253, 313, 473, 1313 |
| BstMBI | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| BstMCI | CGRYCG | 1 cut(s) | 1695 |
| BstNI | CCWGG | 1 cut(s) | 938 |
| BstNSI | RCATGY | 1 cut(s) | 838 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 99 |
| BstSCI | CCNGG | 1 cut(s) | 936 |
| BstSFI | CTRYAG | 2 cut(s) | 1087, 1619 |
| BstV1I | GCAGC | 4 cut(s) | 801, 1030, 1101, 1203 |
| BstV2I | GAAGAC | 1 cut(s) | 95 |
| BstX2I | RGATCY | 1 cut(s) | 859 |
| BstYI | RGATCY | 1 cut(s) | 859 |
| BstZI | CGGCCG | 1 cut(s) | 1692 |
| Bsu15I | ATCGAT | 2 cut(s) | 994, 1050 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1443 |
| BsuI | GTATCC | 1 cut(s) | 1141 |
| BsuRI | GGCC | 5 cut(s) | 598, 947, 1488, 1536, 1694 |
| BsuTUI | ATCGAT | 2 cut(s) | 994, 1050 |
| BtgI | CCRYGG | 1 cut(s) | 1544 |
| BtrI | CACGTC | 1 cut(s) | 680 |
| BtsCI | GGATG | 1 cut(s) | 481 |
| BtsI | GCAGTG | 2 cut(s) | 203, 1723 |
| BtsIMutI | CAGTG | 5 cut(s) | 203, 531, 610, 1210, 1723 |
| Cac8I | GCNNGC | 3 cut(s) | 925, 1061, 1145 |
| Cfr10I | RCCGGY | 1 cut(s) | 1059 |
| Cfr13I | GGNCC | 1 cut(s) | 1173 |
| ClaI | ATCGAT | 2 cut(s) | 994, 1050 |
| CseI | GACGC | 1 cut(s) | 829 |
| Csp6I | GTAC | 7 cut(s) | 33, 227, 386, 979, 1215, 1460, 1508 |
| CviAII | CATG | 8 cut(s) | 42, 631, 748, 835, 943, 1366, 1545, 1590 |
| CviQI | GTAC | 7 cut(s) | 33, 227, 386, 979, 1215, 1460, 1508 |
| DdeI | CTNAG | 2 cut(s) | 969, 1443 |
| DpnI | GATC | 6 cut(s) | 513, 537, 861, 1049, 1202, 1662 |
| DpnII | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| EaeI | YGGCCR | 4 cut(s) | 945, 1486, 1534, 1692 |
| EagI | CGGCCG | 1 cut(s) | 1692 |
| Eam1104I | CTCTTC | 1 cut(s) | 591 |
| EarI | CTCTTC | 1 cut(s) | 591 |
| EciI | GGCGGA | 1 cut(s) | 999 |
| EclXI | CGGCCG | 1 cut(s) | 1692 |
| Eco130I | CCWWGG | 3 cut(s) | 515, 1518, 1544 |
| Eco24I | GRGCYC | 1 cut(s) | 1311 |
| Eco47I | GGWCC | 1 cut(s) | 1173 |
| Eco52I | CGGCCG | 1 cut(s) | 1692 |
| Eco57I | CTGAAG | 1 cut(s) | 372 |
| Eco81I | CCTNAGG | 1 cut(s) | 1443 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 152 |
| EcoRII | CCWGG | 1 cut(s) | 936 |
| EcoT14I | CCWWGG | 3 cut(s) | 515, 1518, 1544 |
| EcoT38I | GRGCYC | 1 cut(s) | 1311 |
| ErhI | CCWWGG | 3 cut(s) | 515, 1518, 1544 |
| FaeI | CATG | 8 cut(s) | 45, 634, 751, 838, 946, 1369, 1548, 1593 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 9, 41 |
| FaqI | GGGAC | 1 cut(s) | 1159 |
| FatI | CATG | 8 cut(s) | 41, 630, 747, 834, 942, 1365, 1544, 1589 |
| FauNDI | CATATG | 1 cut(s) | 1665 |
| FbaI | TGATCA | 2 cut(s) | 535, 1660 |
| FblI | GTMKAC | 1 cut(s) | 6 |
| Fnu4HI | GCNGC | 5 cut(s) | 318, 815, 1019, 1090, 1192 |
| FokI | GGATG | 1 cut(s) | 488 |
| FriOI | GRGCYC | 1 cut(s) | 1311 |
| Fsp4HI | GCNGC | 5 cut(s) | 318, 815, 1019, 1090, 1192 |
| FspBI | CTAG | 5 cut(s) | 303, 797, 1137, 1463, 1511 |
| GluI | GCNGC | 5 cut(s) | 318, 815, 1019, 1090, 1192 |
| GsuI | CTGGAG | 1 cut(s) | 592 |
| HaeIII | GGCC | 5 cut(s) | 598, 947, 1488, 1536, 1694 |
| HapII | CCGG | 1 cut(s) | 1060 |
| HgaI | GACGC | 1 cut(s) | 829 |
| Hin1II | CATG | 8 cut(s) | 45, 634, 751, 838, 946, 1369, 1548, 1593 |
| HincII | GTYRAC | 2 cut(s) | 7, 867 |
| HindII | GTYRAC | 2 cut(s) | 7, 867 |
| HindIII | AAGCTT | 1 cut(s) | 1280 |
| HpaII | CCGG | 1 cut(s) | 1060 |
| HphI | GGTGA | 2 cut(s) | 349, 1693 |
| Hpy166II | GTNNAC | 5 cut(s) | 7, 35, 578, 867, 1217 |
| Hpy188III | TCNNGA | 6 cut(s) | 571, 823, 844, 908, 932, 1137 |
| Hpy8I | GTNNAC | 5 cut(s) | 7, 35, 578, 867, 1217 |
| Hpy99I | CGWCG | 2 cut(s) | 845, 1039 |
| HpyAV | CCTTC | 9 cut(s) | 23, 126, 850, 1143, 1220, 1363, 1625, 1648, 1672 |
| HpyCH4III | ACNGT | 2 cut(s) | 1214, 1353 |
| HpyCH4IV | ACGT | 1 cut(s) | 679 |
| HpyCH4V | TGCA | 7 cut(s) | 187, 817, 1069, 1089, 1112, 1210, 1621 |
| HpyF3I | CTNAG | 2 cut(s) | 969, 1443 |
| HpySE526I | ACGT | 1 cut(s) | 679 |
| Hsp92II | CATG | 8 cut(s) | 45, 634, 751, 838, 946, 1369, 1548, 1593 |
| KroI | GCCGGC | 1 cut(s) | 1059 |
| KroNI | GCCGGC | 1 cut(s) | 1061 |
| Ksp22I | TGATCA | 2 cut(s) | 535, 1660 |
| Kzo9I | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| LmnI | GCTCC | 3 cut(s) | 1188, 1306, 1648 |
| Lsp1109I | GCAGC | 4 cut(s) | 801, 1030, 1101, 1203 |
| LweI | GCATC | 1 cut(s) | 174 |
| MaeI | CTAG | 5 cut(s) | 303, 797, 1137, 1463, 1511 |
| MaeII | ACGT | 1 cut(s) | 679 |
| MaeIII | GTNAC | 3 cut(s) | 355, 1393, 1681 |
| MalI | GATC | 6 cut(s) | 513, 537, 861, 1049, 1202, 1662 |
| MboI | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| MboII | GAAGA | 9 cut(s) | 64, 95, 150, 266, 578, 873, 879, 1013, 1016 |
| MfeI | CAATTG | 1 cut(s) | 872 |
| MflI | RGATCY | 1 cut(s) | 859 |
| MhlI | GDGCHC | 1 cut(s) | 1311 |
| MlsI | TGGCCA | 2 cut(s) | 1488, 1536 |
| MluNI | TGGCCA | 2 cut(s) | 1488, 1536 |
| MlyI | GAGTC | 4 cut(s) | 290, 475, 628, 1315 |
| Mox20I | TGGCCA | 2 cut(s) | 1488, 1536 |
| MroNI | GCCGGC | 1 cut(s) | 1059 |
| MroXI | GAANNNNTTC | 1 cut(s) | 741 |
| MscI | TGGCCA | 2 cut(s) | 1488, 1536 |
| MseI | TTAA | 6 cut(s) | 126, 449, 507, 1157, 1407, 1560 |
| MslI | CAYNNNNRTG | 1 cut(s) | 40 |
| Msp20I | TGGCCA | 2 cut(s) | 1488, 1536 |
| MspA1I | CMGCKG | 1 cut(s) | 923 |
| MspI | CCGG | 1 cut(s) | 1060 |
| MspR9I | CCNGG | 1 cut(s) | 938 |
| MunI | CAATTG | 1 cut(s) | 872 |
| Mva1269I | GAATGC | 1 cut(s) | 927 |
| MvaI | CCWGG | 1 cut(s) | 938 |
| NaeI | GCCGGC | 1 cut(s) | 1061 |
| NcoI | CCATGG | 1 cut(s) | 1544 |
| NdeI | CATATG | 1 cut(s) | 1665 |
| NdeII | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| NgoMIV | GCCGGC | 1 cut(s) | 1059 |
| NlaIII | CATG | 8 cut(s) | 45, 634, 751, 838, 946, 1369, 1548, 1593 |
| NlaIV | GGNNCC | 5 cut(s) | 861, 1041, 1084, 1175, 1308 |
| NmuCI | GTSAC | 2 cut(s) | 355, 1681 |
| NspI | RCATGY | 1 cut(s) | 838 |
| PciI | ACATGT | 1 cut(s) | 834 |
| PctI | GAATGC | 1 cut(s) | 927 |
| PdiI | GCCGGC | 1 cut(s) | 1061 |
| PdmI | GAANNNNTTC | 1 cut(s) | 741 |
| PfeI | GAWTC | 6 cut(s) | 502, 1031, 1385, 1466, 1514, 1688 |
| PkrI | GCNGC | 5 cut(s) | 319, 816, 1020, 1091, 1193 |
| PleI | GAGTC | 4 cut(s) | 290, 475, 628, 1315 |
| PpsI | GAGTC | 4 cut(s) | 290, 475, 628, 1315 |
| PscI | ACATGT | 1 cut(s) | 834 |
| PshAI | GACNNNNGTC | 1 cut(s) | 99 |
| PshBI | ATTAAT | 1 cut(s) | 449 |
| Psp6I | CCWGG | 1 cut(s) | 936 |
| PspGI | CCWGG | 1 cut(s) | 936 |
| PspN4I | GGNNCC | 5 cut(s) | 861, 1041, 1084, 1175, 1308 |
| PspPI | GGNCC | 1 cut(s) | 1173 |
| PstI | CTGCAG | 2 cut(s) | 1091, 1623 |
| PsuI | RGATCY | 1 cut(s) | 859 |
| PvuII | CAGCTG | 1 cut(s) | 923 |
| RsaI | GTAC | 7 cut(s) | 34, 228, 387, 980, 1216, 1461, 1509 |
| RsaNI | GTAC | 7 cut(s) | 33, 227, 386, 979, 1215, 1460, 1508 |
| RseI | CAYNNNNRTG | 1 cut(s) | 40 |
| SalI | GTCGAC | 1 cut(s) | 5 |
| SaqAI | TTAA | 6 cut(s) | 126, 449, 507, 1157, 1407, 1560 |
| SatI | GCNGC | 5 cut(s) | 318, 815, 1019, 1090, 1192 |
| Sau3AI | GATC | 6 cut(s) | 511, 535, 859, 1047, 1200, 1660 |
| Sau96I | GGNCC | 1 cut(s) | 1173 |
| SchI | GAGTC | 4 cut(s) | 290, 475, 628, 1315 |
| ScrFI | CCNGG | 1 cut(s) | 938 |
| SduI | GDGCHC | 1 cut(s) | 1311 |
| SfaNI | GCATC | 1 cut(s) | 174 |
| SfcI | CTRYAG | 2 cut(s) | 1087, 1619 |
| SinI | GGWCC | 1 cut(s) | 1173 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 40 |
| SmlI | CTYRAG | 2 cut(s) | 252, 1195 |
| SmoI | CTYRAG | 2 cut(s) | 252, 1195 |
| SpeI | ACTAGT | 1 cut(s) | 302 |
| SsiI | CCGC | 5 cut(s) | 318, 984, 1153, 1297, 1451 |
| SspI | AATATT | 1 cut(s) | 241 |
| SspMI | CTAG | 5 cut(s) | 303, 797, 1137, 1463, 1511 |
| StyD4I | CCNGG | 1 cut(s) | 936 |
| StyI | CCWWGG | 3 cut(s) | 515, 1518, 1544 |
| TaaI | ACNGT | 2 cut(s) | 1214, 1353 |
| TaiI | ACGT | 1 cut(s) | 682 |
| TaqI | TCGA | 7 cut(s) | 6, 236, 624, 843, 994, 1050, 1709 |
| TatI | WGTACW | 2 cut(s) | 1214, 1507 |
| TauI | GCSGC | 1 cut(s) | 320 |
| TfiI | GAWTC | 6 cut(s) | 502, 1031, 1385, 1466, 1514, 1688 |
| Tru1I | TTAA | 6 cut(s) | 126, 449, 507, 1157, 1407, 1560 |
| Tru9I | TTAA | 6 cut(s) | 126, 449, 507, 1157, 1407, 1560 |
| TscAI | CASTG | 5 cut(s) | 210, 538, 610, 1217, 1723 |
| TseFI | GTSAC | 2 cut(s) | 355, 1681 |
| TseI | GCWGC | 4 cut(s) | 814, 1018, 1089, 1191 |
| Tsp45I | GTSAC | 2 cut(s) | 355, 1681 |
| TspGWI | ACGGA | 2 cut(s) | 852, 1201 |
| TspRI | CASTG | 5 cut(s) | 210, 538, 610, 1217, 1723 |
| VpaK11BI | GGWCC | 1 cut(s) | 1173 |
| VspI | ATTAAT | 1 cut(s) | 449 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 152 |
| XapI | RAATTY | 1 cut(s) | 1629 |
| XbaI | TCTAGA | 1 cut(s) | 1136 |
| XceI | RCATGY | 1 cut(s) | 838 |
| XmiI | GTMKAC | 1 cut(s) | 6 |
| XmnI | GAANNNNTTC | 1 cut(s) | 741 |
| XspI | CTAG | 5 cut(s) | 303, 797, 1137, 1463, 1511 |