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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 233, 561 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1232 |
| AccI | GTMKAC | 1 cut(s) | 162 |
| AccIII | TCCGGA | 1 cut(s) | 1948 |
| AciI | CCGC | 3 cut(s) | 23, 543, 1915 |
| AclWI | GGATC | 8 cut(s) | 647, 802, 829, 1418, 1463, 1501, 1514, 1541 |
| AcoI | YGGCCR | 1 cut(s) | 1783 |
| AcsI | RAATTY | 1 cut(s) | 474 |
| AcuI | CTGAAG | 2 cut(s) | 268, 324 |
| AcyI | GRCGYC | 1 cut(s) | 562 |
| AfaI | GTAC | 6 cut(s) | 301, 1118, 1205, 1217, 1472, 1877 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 402, 1232, 1885 |
| AflII | CTTAAG | 1 cut(s) | 830 |
| AgsI | TTSAA | 7 cut(s) | 251, 375, 934, 1044, 1267, 1804, 1870 |
| AjnI | CCWGG | 2 cut(s) | 564, 1047 |
| AleI | CACNNNNGTG | 1 cut(s) | 1772 |
| Alw21I | GWGCWC | 2 cut(s) | 81, 120 |
| Alw26I | GTCTC | 4 cut(s) | 869, 1867, 1946, 1982 |
| AlwI | GGATC | 8 cut(s) | 647, 802, 829, 1418, 1463, 1501, 1514, 1541 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1232, 1717 |
| Ama87I | CYCGRG | 1 cut(s) | 1631 |
| Aor13HI | TCCGGA | 1 cut(s) | 1948 |
| AoxI | GGCC | 3 cut(s) | 423, 936, 1783 |
| ApeKI | GCWGC | 3 cut(s) | 431, 964, 1717 |
| ApoI | RAATTY | 1 cut(s) | 474 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 137, 169 |
| AspLEI | GCGC | 2 cut(s) | 564, 1604 |
| AspS9I | GGNCC | 5 cut(s) | 688, 937, 1152, 1764, 2024 |
| AsuII | TTCGAA | 1 cut(s) | 1393 |
| AvaI | CYCGRG | 1 cut(s) | 1631 |
| AvaII | GGWCC | 4 cut(s) | 688, 1152, 1764, 2024 |
| AxyI | CCTNAGG | 1 cut(s) | 515 |
| BaeGI | GKGCMC | 2 cut(s) | 236, 528 |
| BalI | TGGCCA | 1 cut(s) | 1785 |
| BamHI | GGATCC | 1 cut(s) | 1506 |
| BanI | GGYRCC | 2 cut(s) | 233, 561 |
| BanII | GRGCYC | 2 cut(s) | 81, 120 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1463, 1495 |
| BauI | CACGAG | 2 cut(s) | 140, 1880 |
| BbsI | GAAGAC | 2 cut(s) | 1389, 1623 |
| Bbv12I | GWGCWC | 2 cut(s) | 81, 120 |
| BbvI | GCAGC | 3 cut(s) | 418, 976, 1704 |
| BccI | CCATC | 6 cut(s) | 536, 971, 1447, 1737, 1821, 2015 |
| BceAI | ACGGC | 4 cut(s) | 575, 1520, 1663, 1804 |
| BciT130I | CCWGG | 2 cut(s) | 566, 1049 |
| BclI | TGATCA | 2 cut(s) | 177, 591 |
| BcoDI | GTCTC | 4 cut(s) | 869, 1867, 1946, 1982 |
| BfmI | CTRYAG | 2 cut(s) | 880, 1278 |
| BfoI | RGCGCY | 1 cut(s) | 565 |
| BfrI | CTTAAG | 1 cut(s) | 830 |
| BglII | AGATCT | 1 cut(s) | 1179 |
| BisI | GCNGC | 4 cut(s) | 432, 965, 1718, 1915 |
| BlpI | GCTNAGC | 2 cut(s) | 5, 1754 |
| BlsI | GCNGC | 4 cut(s) | 433, 966, 1719, 1916 |
| BmcAI | AGTACT | 1 cut(s) | 1217 |
| Bme1390I | CCNGG | 2 cut(s) | 566, 1049 |
| Bme18I | GGWCC | 4 cut(s) | 688, 1152, 1764, 2024 |
| BmeT110I | CYCGRG | 1 cut(s) | 1631 |
| BmgT120I | GGNCC | 5 cut(s) | 688, 937, 1152, 1764, 2024 |
| BmiI | GGNNCC | 8 cut(s) | 235, 464, 563, 982, 1508, 1765, 1947, 2026 |
| BmrFI | CCNGG | 2 cut(s) | 566, 1049 |
| BmrI | ACTGGG | 1 cut(s) | 692 |
| BmsI | GCATC | 3 cut(s) | 630, 1185, 1245 |
| BmuI | ACTGGG | 1 cut(s) | 692 |
| BpiI | GAAGAC | 2 cut(s) | 1389, 1623 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 134, 166 |
| BpmI | CTGGAG | 2 cut(s) | 536, 653 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 5, 1754 |
| Bpu14I | TTCGAA | 1 cut(s) | 1393 |
| BpuEI | CTTGAG | 5 cut(s) | 290, 521, 1204, 1550, 1832 |
| BsaHI | GRCGYC | 1 cut(s) | 562 |
| BsaI | GGTCTC | 3 cut(s) | 869, 1867, 1982 |
| BsaJI | CCNNGG | 6 cut(s) | 411, 426, 1199, 1621, 1786, 1983 |
| BsaWI | WCCGGW | 1 cut(s) | 1948 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1454, 1484 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 402, 1232, 1885 |
| Bse1I | ACTGG | 5 cut(s) | 698, 984, 1085, 1226, 1786 |
| Bse21I | CCTNAGG | 1 cut(s) | 515 |
| Bse3DI | GCAATG | 4 cut(s) | 602, 690, 1068, 1154 |
| BseAI | TCCGGA | 1 cut(s) | 1948 |
| BseBI | CCWGG | 2 cut(s) | 566, 1049 |
| BseDI | CCNNGG | 6 cut(s) | 411, 426, 1199, 1621, 1786, 1983 |
| BseGI | GGATG | 6 cut(s) | 528, 667, 816, 1439, 1748, 1923 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 402, 1232, 1885 |
| BseMI | GCAATG | 4 cut(s) | 602, 690, 1068, 1154 |
| BseMII | CTCAG | 5 cut(s) | 94, 881, 1004, 1093, 1959 |
| BseNI | ACTGG | 5 cut(s) | 698, 984, 1085, 1226, 1786 |
| BseRI | GAGGAG | 3 cut(s) | 881, 1030, 1478 |
| BseSI | GKGCMC | 2 cut(s) | 236, 528 |
| BseXI | GCAGC | 3 cut(s) | 418, 976, 1704 |
| BsgI | GTGCAG | 3 cut(s) | 248, 983, 1232 |
| BshFI | GGCC | 3 cut(s) | 425, 938, 1785 |
| BshNI | GGYRCC | 2 cut(s) | 233, 561 |
| BsiHKAI | GWGCWC | 2 cut(s) | 81, 120 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1631 |
| BsiSI | CCGG | 1 cut(s) | 1949 |
| BslFI | GGGAC | 1 cut(s) | 2010 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 402, 1232, 1885 |
| BsmAI | GTCTC | 4 cut(s) | 869, 1867, 1946, 1982 |
| BsmFI | GGGAC | 1 cut(s) | 2010 |
| BsmI | GAATGC | 2 cut(s) | 25, 1626 |
| BsnI | GGCC | 3 cut(s) | 425, 938, 1785 |
| Bso31I | GGTCTC | 3 cut(s) | 869, 1867, 1982 |
| BsoBI | CYCGRG | 1 cut(s) | 1631 |
| Bsp119I | TTCGAA | 1 cut(s) | 1393 |
| Bsp1286I | GDGCHC | 4 cut(s) | 81, 120, 236, 528 |
| Bsp13I | TCCGGA | 1 cut(s) | 1948 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 5, 1754 |
| Bsp19I | CCATGG | 2 cut(s) | 1199, 1983 |
| BspACI | CCGC | 3 cut(s) | 23, 543, 1915 |
| BspANI | GGCC | 3 cut(s) | 425, 938, 1785 |
| BspCNI | CTCAG | 5 cut(s) | 93, 882, 1005, 1092, 1960 |
| BspEI | TCCGGA | 1 cut(s) | 1948 |
| BspLI | GGNNCC | 8 cut(s) | 235, 464, 563, 982, 1508, 1765, 1947, 2026 |
| BspMAI | CTGCAG | 1 cut(s) | 1282 |
| BspPI | GGATC | 8 cut(s) | 647, 802, 829, 1418, 1463, 1501, 1514, 1541 |
| BspT104I | TTCGAA | 1 cut(s) | 1393 |
| BspT107I | GGYRCC | 2 cut(s) | 233, 561 |
| BspTI | CTTAAG | 1 cut(s) | 830 |
| BspTNI | GGTCTC | 3 cut(s) | 869, 1867, 1982 |
| BsrDI | GCAATG | 4 cut(s) | 602, 690, 1068, 1154 |
| BsrI | ACTGG | 5 cut(s) | 698, 984, 1085, 1226, 1786 |
| BssECI | CCNNGG | 6 cut(s) | 411, 426, 1199, 1621, 1786, 1983 |
| BssNI | GRCGYC | 1 cut(s) | 562 |
| BssSI | CACGAG | 2 cut(s) | 140, 1880 |
| BssT1I | CCWWGG | 5 cut(s) | 411, 426, 1199, 1621, 1983 |
| Bst2BI | CACGAG | 2 cut(s) | 140, 1880 |
| Bst2UI | CCWGG | 2 cut(s) | 566, 1049 |
| Bst4CI | ACNGT | 7 cut(s) | 46, 58, 997, 1210, 1696, 1771, 1910 |
| Bst6I | CTCTTC | 2 cut(s) | 342, 1248 |
| BstACI | GRCGYC | 1 cut(s) | 562 |
| BstAFI | CTTAAG | 1 cut(s) | 830 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 20 |
| BstBI | TTCGAA | 1 cut(s) | 1393 |
| BstC8I | GCNNGC | 5 cut(s) | 280, 543, 669, 1234, 1278 |
| BstDEI | CTNAG | 9 cut(s) | 5, 80, 515, 890, 1013, 1079, 1191, 1754, 1968 |
| BstDSI | CCRYGG | 3 cut(s) | 1199, 1786, 1983 |
| BstEII | GGTNACC | 2 cut(s) | 34, 997 |
| BstF5I | GGATG | 6 cut(s) | 528, 667, 816, 1439, 1748, 1923 |
| BstH2I | RGCGCY | 1 cut(s) | 565 |
| BstHHI | GCGC | 2 cut(s) | 564, 1604 |
| BstMAI | GTCTC | 4 cut(s) | 869, 1867, 1946, 1982 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 20, 422, 431, 437, 637 |
| BstNI | CCWGG | 2 cut(s) | 566, 1049 |
| BstPI | GGTNACC | 2 cut(s) | 34, 997 |
| BstSCI | CCNGG | 2 cut(s) | 564, 1047 |
| BstSFI | CTRYAG | 2 cut(s) | 880, 1278 |
| BstSLI | GKGCMC | 2 cut(s) | 236, 528 |
| BstV1I | GCAGC | 3 cut(s) | 418, 976, 1704 |
| BstV2I | GAAGAC | 2 cut(s) | 1389, 1623 |
| BstX2I | RGATCY | 4 cut(s) | 807, 834, 1179, 1506 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1774, 2021 |
| BstYI | RGATCY | 4 cut(s) | 807, 834, 1179, 1506 |
| Bsu36I | CCTNAGG | 1 cut(s) | 515 |
| BsuRI | GGCC | 3 cut(s) | 425, 938, 1785 |
| BtgI | CCRYGG | 3 cut(s) | 1199, 1786, 1983 |
| BtgZI | GCGATG | 1 cut(s) | 780 |
| BtsCI | GGATG | 6 cut(s) | 528, 667, 816, 1439, 1748, 1923 |
| BtsIMutI | CAGTG | 6 cut(s) | 54, 1215, 1767, 1779, 1906, 2016 |
| Cac8I | GCNNGC | 5 cut(s) | 280, 543, 669, 1234, 1278 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1232, 1717 |
| CfoI | GCGC | 2 cut(s) | 564, 1604 |
| Cfr13I | GGNCC | 5 cut(s) | 688, 937, 1152, 1764, 2024 |
| Csp6I | GTAC | 6 cut(s) | 300, 1117, 1204, 1216, 1471, 1876 |
| CviAII | CATG | 7 cut(s) | 94, 134, 245, 782, 1200, 1452, 1984 |
| CviQI | GTAC | 6 cut(s) | 300, 1117, 1204, 1216, 1471, 1876 |
| DdeI | CTNAG | 9 cut(s) | 5, 80, 515, 890, 1013, 1079, 1191, 1754, 1968 |
| DinI | GGCGCC | 1 cut(s) | 563 |
| EaeI | YGGCCR | 1 cut(s) | 1783 |
| Eam1104I | CTCTTC | 2 cut(s) | 342, 1248 |
| EarI | CTCTTC | 2 cut(s) | 342, 1248 |
| Ecl136II | GAGCTC | 2 cut(s) | 79, 118 |
| Eco130I | CCWWGG | 5 cut(s) | 411, 426, 1199, 1621, 1983 |
| Eco24I | GRGCYC | 2 cut(s) | 81, 120 |
| Eco31I | GGTCTC | 3 cut(s) | 869, 1867, 1982 |
| Eco47I | GGWCC | 4 cut(s) | 688, 1152, 1764, 2024 |
| Eco53kI | GAGCTC | 2 cut(s) | 79, 118 |
| Eco57I | CTGAAG | 2 cut(s) | 268, 324 |
| Eco81I | CCTNAGG | 1 cut(s) | 515 |
| Eco88I | CYCGRG | 1 cut(s) | 1631 |
| Eco91I | GGTNACC | 2 cut(s) | 34, 997 |
| EcoICRI | GAGCTC | 2 cut(s) | 79, 118 |
| EcoO109I | RGGNCCY | 1 cut(s) | 937 |
| EcoO65I | GGTNACC | 2 cut(s) | 34, 997 |
| EcoRII | CCWGG | 2 cut(s) | 564, 1047 |
| EcoT14I | CCWWGG | 5 cut(s) | 411, 426, 1199, 1621, 1983 |
| EcoT38I | GRGCYC | 2 cut(s) | 81, 120 |
| EgeI | GGCGCC | 1 cut(s) | 563 |
| EheI | GGCGCC | 1 cut(s) | 563 |
| ErhI | CCWWGG | 5 cut(s) | 411, 426, 1199, 1621, 1983 |
| FaeI | CATG | 7 cut(s) | 97, 137, 248, 785, 1203, 1455, 1987 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 404, 436, 738, 770 |
| FaqI | GGGAC | 1 cut(s) | 2010 |
| FatI | CATG | 7 cut(s) | 93, 133, 244, 781, 1199, 1451, 1983 |
| FauI | CCCGC | 1 cut(s) | 550 |
| FbaI | TGATCA | 2 cut(s) | 177, 591 |
| FblI | GTMKAC | 1 cut(s) | 162 |
| Fnu4HI | GCNGC | 4 cut(s) | 432, 965, 1718, 1915 |
| FokI | GGATG | 6 cut(s) | 515, 674, 803, 1426, 1755, 1910 |
| FriOI | GRGCYC | 2 cut(s) | 81, 120 |
| Fsp4HI | GCNGC | 4 cut(s) | 432, 965, 1718, 1915 |
| GlaI | GCGC | 2 cut(s) | 563, 1603 |
| GluI | GCNGC | 4 cut(s) | 432, 965, 1718, 1915 |
| GsuI | CTGGAG | 2 cut(s) | 536, 653 |
| HaeII | RGCGCY | 1 cut(s) | 565 |
| HaeIII | GGCC | 3 cut(s) | 425, 938, 1785 |
| HapII | CCGG | 1 cut(s) | 1949 |
| HhaI | GCGC | 2 cut(s) | 564, 1604 |
| Hin1I | GRCGYC | 1 cut(s) | 562 |
| Hin1II | CATG | 7 cut(s) | 97, 137, 248, 785, 1203, 1455, 1987 |
| Hin6I | GCGC | 2 cut(s) | 562, 1602 |
| HinP1I | GCGC | 2 cut(s) | 562, 1602 |
| HincII | GTYRAC | 2 cut(s) | 163, 1678 |
| HindII | GTYRAC | 2 cut(s) | 163, 1678 |
| HindIII | AAGCTT | 2 cut(s) | 638, 827 |
| HinfI | GANTC | 6 cut(s) | 149, 164, 1132, 1336, 1657, 1891 |
| HpaII | CCGG | 1 cut(s) | 1949 |
| Hpy166II | GTNNAC | 8 cut(s) | 90, 139, 163, 300, 751, 1206, 1678, 1767 |
| Hpy188I | TCNGA | 9 cut(s) | 100, 487, 591, 906, 1014, 1060, 1082, 1449, 1896 |
| Hpy8I | GTNNAC | 8 cut(s) | 90, 139, 163, 300, 751, 1206, 1678, 1767 |
| HpyAV | CCTTC | 6 cut(s) | 542, 764, 1348, 1798, 1825, 1879 |
| HpyCH4III | ACNGT | 7 cut(s) | 46, 58, 997, 1210, 1696, 1771, 1910 |
| HpyCH4IV | ACGT | 1 cut(s) | 1008 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 20, 422, 431, 437, 637 |
| HpyF3I | CTNAG | 9 cut(s) | 5, 80, 515, 890, 1013, 1079, 1191, 1754, 1968 |
| HpySE526I | ACGT | 1 cut(s) | 1008 |
| Hsp92I | GRCGYC | 1 cut(s) | 562 |
| Hsp92II | CATG | 7 cut(s) | 97, 137, 248, 785, 1203, 1455, 1987 |
| HspAI | GCGC | 2 cut(s) | 562, 1602 |
| KasI | GGCGCC | 1 cut(s) | 561 |
| Kpn2I | TCCGGA | 1 cut(s) | 1948 |
| Ksp22I | TGATCA | 2 cut(s) | 177, 591 |
| LmnI | GCTCC | 4 cut(s) | 115, 293, 468, 1144 |
| Lsp1109I | GCAGC | 3 cut(s) | 418, 976, 1704 |
| LweI | GCATC | 3 cut(s) | 630, 1185, 1245 |
| MaeII | ACGT | 1 cut(s) | 1008 |
| MaeIII | GTNAC | 6 cut(s) | 34, 46, 986, 997, 1696, 1777 |
| MboII | GAAGA | 6 cut(s) | 359, 1265, 1394, 1471, 1628, 1657 |
| MflI | RGATCY | 4 cut(s) | 807, 834, 1179, 1506 |
| MhlI | GDGCHC | 4 cut(s) | 81, 120, 236, 528 |
| MlsI | TGGCCA | 1 cut(s) | 1785 |
| MluCI | AATT | 5 cut(s) | 474, 972, 1074, 1122, 1514 |
| MluNI | TGGCCA | 1 cut(s) | 1785 |
| Mly113I | GGCGCC | 1 cut(s) | 562 |
| MlyI | GAGTC | 3 cut(s) | 143, 158, 1666 |
| Mox20I | TGGCCA | 1 cut(s) | 1785 |
| MroI | TCCGGA | 1 cut(s) | 1948 |
| MscI | TGGCCA | 1 cut(s) | 1785 |
| MseI | TTAA | 5 cut(s) | 582, 831, 1248, 1557, 2031 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1772, 2019 |
| Msp20I | TGGCCA | 1 cut(s) | 1785 |
| MspA1I | CMGCKG | 2 cut(s) | 545, 841 |
| MspCI | CTTAAG | 1 cut(s) | 830 |
| MspI | CCGG | 1 cut(s) | 1949 |
| MspR9I | CCNGG | 2 cut(s) | 566, 1049 |
| Mva1269I | GAATGC | 2 cut(s) | 25, 1626 |
| MvaI | CCWGG | 2 cut(s) | 566, 1049 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 20, 422, 431, 437, 637 |
| NarI | GGCGCC | 1 cut(s) | 562 |
| NcoI | CCATGG | 2 cut(s) | 1199, 1983 |
| NlaIII | CATG | 7 cut(s) | 97, 137, 248, 785, 1203, 1455, 1987 |
| NlaIV | GGNNCC | 8 cut(s) | 235, 464, 563, 982, 1508, 1765, 1947, 2026 |
| NmuCI | GTSAC | 4 cut(s) | 34, 46, 1696, 1777 |
| NspV | TTCGAA | 1 cut(s) | 1393 |
| OliI | CACNNNNGTG | 1 cut(s) | 1772 |
| PctI | GAATGC | 2 cut(s) | 25, 1626 |
| PfeI | GAWTC | 3 cut(s) | 1132, 1336, 1891 |
| PflFI | GACNNNGTC | 1 cut(s) | 1694 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1232 |
| PkrI | GCNGC | 4 cut(s) | 433, 966, 1719, 1916 |
| PleI | GAGTC | 3 cut(s) | 143, 158, 1665 |
| PluTI | GGCGCC | 1 cut(s) | 565 |
| PpsI | GAGTC | 3 cut(s) | 143, 158, 1665 |
| Psp124BI | GAGCTC | 2 cut(s) | 81, 120 |
| Psp6I | CCWGG | 2 cut(s) | 564, 1047 |
| PspEI | GGTNACC | 2 cut(s) | 34, 997 |
| PspGI | CCWGG | 2 cut(s) | 564, 1047 |
| PspN4I | GGNNCC | 8 cut(s) | 235, 464, 563, 982, 1508, 1765, 1947, 2026 |
| PspPI | GGNCC | 5 cut(s) | 688, 937, 1152, 1764, 2024 |
| PstI | CTGCAG | 1 cut(s) | 1282 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1232, 1717 |
| PsuI | RGATCY | 4 cut(s) | 807, 834, 1179, 1506 |
| PsyI | GACNNNGTC | 1 cut(s) | 1694 |
| PvuII | CAGCTG | 1 cut(s) | 841 |
| RsaI | GTAC | 6 cut(s) | 301, 1118, 1205, 1217, 1472, 1877 |
| RsaNI | GTAC | 6 cut(s) | 300, 1117, 1204, 1216, 1471, 1876 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1772, 2019 |
| SacI | GAGCTC | 2 cut(s) | 81, 120 |
| SalI | GTCGAC | 1 cut(s) | 161 |
| SaqAI | TTAA | 5 cut(s) | 582, 831, 1248, 1557, 2031 |
| SatI | GCNGC | 4 cut(s) | 432, 965, 1718, 1915 |
| Sau96I | GGNCC | 5 cut(s) | 688, 937, 1152, 1764, 2024 |
| ScaI | AGTACT | 1 cut(s) | 1217 |
| SchI | GAGTC | 3 cut(s) | 143, 158, 1666 |
| ScrFI | CCNGG | 2 cut(s) | 566, 1049 |
| SduI | GDGCHC | 4 cut(s) | 81, 120, 236, 528 |
| SfaNI | GCATC | 3 cut(s) | 630, 1185, 1245 |
| SfcI | CTRYAG | 2 cut(s) | 880, 1278 |
| SfoI | GGCGCC | 1 cut(s) | 563 |
| SfuI | TTCGAA | 1 cut(s) | 1393 |
| SinI | GGWCC | 4 cut(s) | 688, 1152, 1764, 2024 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1772, 2019 |
| SmlI | CTYRAG | 6 cut(s) | 305, 536, 830, 1183, 1565, 1811 |
| SmoI | CTYRAG | 6 cut(s) | 305, 536, 830, 1183, 1565, 1811 |
| Sse9I | AATT | 5 cut(s) | 474, 972, 1074, 1122, 1514 |
| SsiI | CCGC | 3 cut(s) | 23, 543, 1915 |
| SspDI | GGCGCC | 1 cut(s) | 561 |
| SspI | AATATT | 1 cut(s) | 797 |
| SstI | GAGCTC | 2 cut(s) | 81, 120 |
| StyD4I | CCNGG | 2 cut(s) | 564, 1047 |
| StyI | CCWWGG | 5 cut(s) | 411, 426, 1199, 1621, 1983 |
| TaaI | ACNGT | 7 cut(s) | 46, 58, 997, 1210, 1696, 1771, 1910 |
| TaiI | ACGT | 1 cut(s) | 1011 |
| TaqI | TCGA | 5 cut(s) | 41, 162, 576, 1176, 1393 |
| TaqII | GACCGA | 1 cut(s) | 1169 |
| TasI | AATT | 5 cut(s) | 474, 972, 1074, 1122, 1514 |
| TatI | WGTACW | 3 cut(s) | 1116, 1215, 1470 |
| TauI | GCSGC | 1 cut(s) | 1917 |
| TfiI | GAWTC | 3 cut(s) | 1132, 1336, 1891 |
| Tru1I | TTAA | 5 cut(s) | 582, 831, 1248, 1557, 2031 |
| Tru9I | TTAA | 5 cut(s) | 582, 831, 1248, 1557, 2031 |
| TscAI | CASTG | 6 cut(s) | 61, 1215, 1774, 1786, 1913, 2023 |
| TseFI | GTSAC | 4 cut(s) | 34, 46, 1696, 1777 |
| TseI | GCWGC | 3 cut(s) | 431, 964, 1717 |
| Tsp45I | GTSAC | 4 cut(s) | 34, 46, 1696, 1777 |
| TspDTI | ATGAA | 9 cut(s) | 233, 805, 1027, 1234, 1307, 1367, 1515, 1916, 1973 |
| TspGWI | ACGGA | 2 cut(s) | 1500, 1703 |
| TspRI | CASTG | 6 cut(s) | 61, 1215, 1774, 1786, 1913, 2023 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1694 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1232 |
| Vha464I | CTTAAG | 1 cut(s) | 830 |
| VpaK11BI | GGWCC | 4 cut(s) | 688, 1152, 1764, 2024 |
| XapI | RAATTY | 1 cut(s) | 474 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 418 |
| XmiI | GTMKAC | 1 cut(s) | 162 |
| ZrmI | AGTACT | 1 cut(s) | 1217 |