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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 41 |
| AjnI | CCWGG | 1 cut(s) | 74 |
| BbsI | GAAGAC | 2 cut(s) | 68, 128 |
| BciT130I | CCWGG | 1 cut(s) | 76 |
| Bme1390I | CCNGG | 1 cut(s) | 76 |
| BmiI | GGNNCC | 1 cut(s) | 73 |
| BmrFI | CCNGG | 1 cut(s) | 76 |
| BmsI | GCATC | 1 cut(s) | 115 |
| BpiI | GAAGAC | 2 cut(s) | 68, 128 |
| BpmI | CTGGAG | 2 cut(s) | 90, 97 |
| BseBI | CCWGG | 1 cut(s) | 76 |
| BspLI | GGNNCC | 1 cut(s) | 73 |
| Bst2UI | CCWGG | 1 cut(s) | 76 |
| BstNI | CCWGG | 1 cut(s) | 76 |
| BstSCI | CCNGG | 1 cut(s) | 74 |
| BstV2I | GAAGAC | 2 cut(s) | 68, 128 |
| CviAII | CATG | 1 cut(s) | 12 |
| CviJI | RGCY | 1 cut(s) | 74 |
| CviKI_1 | RGCY | 1 cut(s) | 74 |
| Eco57I | CTGAAG | 1 cut(s) | 41 |
| EcoRII | CCWGG | 1 cut(s) | 74 |
| EcoT22I | ATGCAT | 1 cut(s) | 108 |
| FaeI | CATG | 1 cut(s) | 15 |
| FaiI | YATR | 2 cut(s) | 13, 104 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 14, 46 |
| FatI | CATG | 1 cut(s) | 11 |
| GsuI | CTGGAG | 2 cut(s) | 90, 97 |
| Hin1II | CATG | 1 cut(s) | 15 |
| Hpy188III | TCNNGA | 2 cut(s) | 110, 138 |
| HpyAV | CCTTC | 1 cut(s) | 16 |
| HpyCH4V | TGCA | 1 cut(s) | 106 |
| Hsp92II | CATG | 1 cut(s) | 15 |
| LmnI | GCTCC | 1 cut(s) | 71 |
| LpnPI | CCDG | 5 cut(s) | 32, 54, 61, 88, 103 |
| LweI | GCATC | 1 cut(s) | 115 |
| MboII | GAAGA | 2 cut(s) | 73, 133 |
| MnlI | CCTC | 2 cut(s) | 43, 72 |
| Mph1103I | ATGCAT | 1 cut(s) | 108 |
| MseI | TTAA | 2 cut(s) | 32, 83 |
| MspR9I | CCNGG | 1 cut(s) | 76 |
| MvaI | CCWGG | 1 cut(s) | 76 |
| NlaIII | CATG | 1 cut(s) | 15 |
| NlaIV | GGNNCC | 1 cut(s) | 73 |
| NsiI | ATGCAT | 1 cut(s) | 108 |
| Psp6I | CCWGG | 1 cut(s) | 74 |
| PspGI | CCWGG | 1 cut(s) | 74 |
| PspN4I | GGNNCC | 1 cut(s) | 73 |
| SaqAI | TTAA | 2 cut(s) | 32, 83 |
| ScrFI | CCNGG | 1 cut(s) | 76 |
| SetI | ASST | 3 cut(s) | 21, 83, 91 |
| SfaNI | GCATC | 1 cut(s) | 115 |
| SgeI | CNNG | 7 cut(s) | 24, 31, 81, 87, 88, 122, 130 |
| StyD4I | CCNGG | 1 cut(s) | 74 |
| Tru1I | TTAA | 2 cut(s) | 32, 83 |
| Tru9I | TTAA | 2 cut(s) | 32, 83 |
| Zsp2I | ATGCAT | 1 cut(s) | 108 |