Rmu_sc0004250.1_g000026

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004250.1
OG0018669
Physical Location & Seq
Forward (+)
119855 .. 121083
1229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004250.1_g000026.1.cds

Sequence Viewer

Length: 648 bp
atggaggttaatgagatagcgcaggtcctgctgcagatccaagatgaggaagtctttccgcgacagaagttccaggctcatgccccgccgccagaggcgccggctcatgagctggaacagtttaaaagaagacttgctggtttggatttcccaagcaaagtttttgaggtggtggactatcctccattggattactgcattcgttggaacactgaaggcacaggcctgcagattgtgtcggacgctgatttccgcaaggggttcaagaaaaatacaaaatccaggtgcaagcacgagtacagtcatgaaaaagggtacttcctcagagcccaaaaacaaaagttgaagaaaatctgccgcaagccaccacaggccaccgggaagactccgggagagggaagggaggacccattgctcaatgtggaagggcgactgacctctctggaggcaaatatgacttgtcttcaggcaatgtccaatgttgaggcatggggaatggaccagtggctccgtgtggaagggcgacaaacgtcgctagaggcaaaattgacttctctcgatgcaaagtccgaccggatgatctctgaaatggttgcaatctggcaacttgttgaatcacgagctcaccgcctcaccccacatggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

215

Amino Acids

24.65

Weight (kDa)

8.3

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 1 cut(s) 61
AciI CCGC 6 cut(s) 59, 86, 89, 253, 358, 628
AclWI GGATC 1 cut(s) 31
AcuI CTGAAG 2 cut(s) 234, 449
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 2 cut(s) 299, 317
AfiI CCNNNNNNNGG 2 cut(s) 46, 395
AgsI TTSAA 3 cut(s) 265, 346, 614
AjnI CCWGG 2 cut(s) 72, 281
AluBI AGCT 2 cut(s) 112, 623
AluI AGCT 2 cut(s) 112, 623
Alw21I GWGCWC 1 cut(s) 625
AlwI GGATC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 28
AoxI GGCC 2 cut(s) 223, 372
ApeKI GCWGC 1 cut(s) 31
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 2 cut(s) 22, 100
AspS9I GGNCC 3 cut(s) 25, 406, 499
AsuC2I CCSGG 2 cut(s) 379, 390
AsuHPI GGTGA 2 cut(s) 617, 625
AvaII GGWCC 3 cut(s) 25, 406, 499
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 2 cut(s) 331, 625
BauI CACGAG 2 cut(s) 293, 618
BbsI GAAGAC 3 cut(s) 136, 389, 455
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 1 cut(s) 18
BciT130I CCWGG 2 cut(s) 74, 283
BcnI CCSGG 2 cut(s) 379, 390
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 2 cut(s) 32, 227
BfoI RGCGCY 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 13
BisI GCNGC 3 cut(s) 32, 89, 358
BlsI GCNGC 3 cut(s) 33, 90, 359
Bme1390I CCNGG 4 cut(s) 74, 283, 379, 390
Bme18I GGWCC 3 cut(s) 25, 406, 499
BmgT120I GGNCC 3 cut(s) 25, 406, 499
BmiI GGNNCC 3 cut(s) 99, 408, 509
BmrFI CCNGG 4 cut(s) 74, 283, 379, 390
BmsI GCATC 1 cut(s) 550
BoxI GACNNNNGTC 1 cut(s) 529
BpiI GAAGAC 3 cut(s) 136, 389, 455
BpmI CTGGAG 1 cut(s) 464
BpuMI CCSGG 2 cut(s) 379, 390
BsaHI GRCGYC 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 573
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 395
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 502
Bse3DI GCAATG 2 cut(s) 410, 477
BseBI CCWGG 2 cut(s) 74, 283
BseGI GGATG 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 46, 395
BseMI GCAATG 2 cut(s) 410, 477
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 502
BseXI GCAGC 1 cut(s) 18
Bsh1236I CGCG 1 cut(s) 61
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 2 cut(s) 225, 374
BshNI GGYRCC 1 cut(s) 97
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 4 cut(s) 101, 378, 389, 574
BslI CCNNNNNNNGG 2 cut(s) 46, 395
BsmI GAATGC 1 cut(s) 198
BsnI GGCC 2 cut(s) 225, 374
Bsp1286I GDGCHC 2 cut(s) 331, 625
Bsp143I GATC 2 cut(s) 36, 579
BspACI CCGC 6 cut(s) 59, 86, 89, 253, 358, 628
BspANI GGCC 2 cut(s) 225, 374
BspCNI CTCAG 1 cut(s) 336
BspFNI CGCG 1 cut(s) 61
BspHI TCATGA 2 cut(s) 106, 304
BspLI GGNNCC 3 cut(s) 99, 408, 509
BspMAI CTGCAG 2 cut(s) 36, 231
BspMI ACCTGC 1 cut(s) 13
BspPI GGATC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 97
BsrDI GCAATG 2 cut(s) 410, 477
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 1 cut(s) 100
BssMI GATC 2 cut(s) 36, 579
BssNI GRCGYC 1 cut(s) 98
BssSI CACGAG 2 cut(s) 293, 618
Bst2BI CACGAG 2 cut(s) 293, 618
Bst2UI CCWGG 2 cut(s) 74, 283
Bst4CI ACNGT 2 cut(s) 120, 302
BstACI GRCGYC 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 28
BstC8I GCNNGC 4 cut(s) 102, 227, 290, 362
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 1 cut(s) 582
BstFNI CGCG 1 cut(s) 61
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 2 cut(s) 22, 100
BstKTI GATC 2 cut(s) 39, 582
BstMBI GATC 2 cut(s) 36, 579
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 2 cut(s) 28, 97
BstNI CCWGG 2 cut(s) 74, 283
BstPAI GACNNNNGTC 1 cut(s) 529
BstSCI CCNGG 4 cut(s) 72, 281, 377, 388
BstSFI CTRYAG 2 cut(s) 32, 227
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 3 cut(s) 136, 389, 455
BstX2I RGATCY 1 cut(s) 36
BstYI RGATCY 1 cut(s) 36
BsuRI GGCC 2 cut(s) 225, 374
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 210, 509
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 4 cut(s) 102, 227, 290, 362
CaiI CAGNNNCTG 1 cut(s) 28
CciI TCATGA 2 cut(s) 106, 304
CfoI GCGC 2 cut(s) 22, 100
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 3 cut(s) 25, 406, 499
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 298, 316
CviAII CATG 5 cut(s) 80, 107, 305, 489, 641
CviJI RGCY 9 cut(s) 77, 104, 112, 225, 329, 364, 374, 508, 623
CviKI_1 RGCY 9 cut(s) 77, 104, 112, 225, 329, 364, 374, 508, 623
CviQI GTAC 2 cut(s) 298, 316
DdeI CTNAG 1 cut(s) 323
DinI GGCGCC 1 cut(s) 99
DpnI GATC 2 cut(s) 38, 581
DpnII GATC 2 cut(s) 36, 579
DraI TTTAAA 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 623
Eco147I AGGCCT 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 331, 625
Eco47I GGWCC 3 cut(s) 25, 406, 499
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 2 cut(s) 234, 449
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 2 cut(s) 25, 406
EcoRII CCWGG 2 cut(s) 72, 281
EcoT38I GRGCYC 2 cut(s) 331, 625
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
FaeI CATG 5 cut(s) 83, 110, 308, 492, 644
FaiI YATR 6 cut(s) 81, 108, 306, 455, 490, 642
FatI CATG 5 cut(s) 79, 106, 304, 488, 640
FauI CCCGC 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 32, 89, 358
FokI GGATG 1 cut(s) 589
FriOI GRGCYC 2 cut(s) 331, 625
Fsp4HI GCNGC 3 cut(s) 32, 89, 358
FspBI CTAG 1 cut(s) 536
GlaI GCGC 2 cut(s) 21, 99
GluI GCNGC 3 cut(s) 32, 89, 358
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 225, 374
HapII CCGG 4 cut(s) 101, 378, 389, 574
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 22, 100
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 5 cut(s) 83, 110, 308, 492, 644
Hin6I GCGC 2 cut(s) 20, 98
HinP1I GCGC 2 cut(s) 20, 98
HinfI GANTC 2 cut(s) 385, 614
HpaII CCGG 4 cut(s) 101, 378, 389, 574
HphI GGTGA 2 cut(s) 617, 625
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 4 cut(s) 241, 326, 571, 586
Hpy188III TCNNGA 6 cut(s) 107, 265, 305, 443, 557, 618
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 535
HpyAV CCTTC 4 cut(s) 209, 393, 419, 512
HpyCH4III ACNGT 2 cut(s) 120, 302
HpyCH4IV ACGT 1 cut(s) 530
HpyCH4V TGCA 6 cut(s) 34, 198, 229, 288, 563, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 97
HpyF3I CTNAG 1 cut(s) 323
HpySE526I ACGT 1 cut(s) 530
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 5 cut(s) 83, 110, 308, 492, 644
HspAI GCGC 2 cut(s) 20, 98
KasI GGCGCC 1 cut(s) 97
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
Kzo9I GATC 2 cut(s) 36, 579
LmnI GCTCC 1 cut(s) 513
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 550
MaeI CTAG 1 cut(s) 536
MaeII ACGT 1 cut(s) 530
MalI GATC 2 cut(s) 38, 581
MboI GATC 2 cut(s) 36, 579
MboII GAAGA 4 cut(s) 141, 358, 394, 455
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 2 cut(s) 331, 625
MluCI AATT 1 cut(s) 545
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 3 cut(s) 185, 219, 594
MroNI GCCGGC 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 2 cut(s) 9, 123
MspI CCGG 4 cut(s) 101, 378, 389, 574
MspR9I CCNGG 4 cut(s) 74, 283, 379, 390
Mva1269I GAATGC 1 cut(s) 198
MvaI CCWGG 2 cut(s) 74, 283
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 2 cut(s) 28, 97
NaeI GCCGGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 2 cut(s) 379, 390
NdeII GATC 2 cut(s) 36, 579
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 5 cut(s) 83, 110, 308, 492, 644
NlaIV GGNNCC 3 cut(s) 99, 408, 509
PagI TCATGA 2 cut(s) 106, 304
PceI AGGCCT 1 cut(s) 225
PctI GAATGC 1 cut(s) 198
PdiI GCCGGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 1 cut(s) 614
PfoI TCCNGGA 1 cut(s) 388
PkrI GCNGC 3 cut(s) 33, 90, 359
PleI GAGTC 1 cut(s) 379
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 379
PpuMI RGGWCCY 2 cut(s) 25, 406
PshAI GACNNNNGTC 1 cut(s) 529
Psp124BI GAGCTC 1 cut(s) 625
Psp5II RGGWCCY 2 cut(s) 25, 406
Psp6I CCWGG 2 cut(s) 72, 281
PspGI CCWGG 2 cut(s) 72, 281
PspN4I GGNNCC 3 cut(s) 99, 408, 509
PspPI GGNCC 3 cut(s) 25, 406, 499
PspPPI RGGWCCY 2 cut(s) 25, 406
PstI CTGCAG 2 cut(s) 36, 231
PstNI CAGNNNCTG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 36
RsaI GTAC 2 cut(s) 299, 317
RsaNI GTAC 2 cut(s) 298, 316
SacI GAGCTC 1 cut(s) 625
SaqAI TTAA 2 cut(s) 9, 123
SatI GCNGC 3 cut(s) 32, 89, 358
Sau3AI GATC 2 cut(s) 36, 579
Sau96I GGNCC 3 cut(s) 25, 406, 499
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 4 cut(s) 74, 283, 379, 390
SduI GDGCHC 2 cut(s) 331, 625
SetI ASST 8 cut(s) 9, 27, 114, 171, 287, 440, 533, 625
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 2 cut(s) 32, 227
SfoI GGCGCC 1 cut(s) 99
SinI GGWCC 3 cut(s) 25, 406, 499
Sse9I AATT 1 cut(s) 545
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 6 cut(s) 59, 86, 89, 253, 358, 628
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 1 cut(s) 536
SstI GAGCTC 1 cut(s) 625
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 4 cut(s) 72, 281, 377, 388
TaaI ACNGT 2 cut(s) 120, 302
TaiI ACGT 1 cut(s) 533
TaqI TCGA 1 cut(s) 558
TasI AATT 1 cut(s) 545
TatI WGTACW 1 cut(s) 297
TauI GCSGC 2 cut(s) 91, 360
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 2 cut(s) 9, 123
Tru9I TTAA 2 cut(s) 9, 123
TscAI CASTG 2 cut(s) 217, 509
TseI GCWGC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 321
TspGWI ACGGA 1 cut(s) 500
TspRI CASTG 2 cut(s) 217, 509
VpaK11BI GGWCC 3 cut(s) 25, 406, 499
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.