Rmu_sc0005297.1_g000025

Peptide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005297.1
OG0017245
Physical Location & Seq
Forward (+)
101815 .. 106565
4751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005297.1_g000025.1.cds

Sequence Viewer

Length: 1872 bp
atggaagagcaactgggatgttgcaattctagtgatggaagagcgatggggacacctagtgaggcacttggaaatatgtgcggatatacactacctagctccaaaattatggcaaaagtagagaagaaatgccttgagaatgctggattactagattacacagaagatgggactacagacctcaaaggtagacctattacaagatcaaagactgggagatggagagcttgctccttccttgtagggtatgaaatatttgagaggatggcaaatcatgggatttcagcaaacctagtggtgtatttggcaaggaagctccatgaaggcactgtaaaatcttcaaacaatgtcaccaactggactggtaccctatctctcacaccgatattaggcgcttatgttgcagatacatatcttggccgatattggacctttgtttctgcatcagccatttatataatgggaatgtcactactaaccctagcagtatcagtgccagctctgagaccaccatcgtgcggccctggaatcaaggacgaggactgcacattaagagcttcgccattccaagtagggattttctattgtgcattgtacatgatagcggttggaattggtggaacaaaacccaatatctcaaccatgggggcagaccaatttgacagttttgagccaaaggagaagcatcagaagctttccttctttaattggtggatgttttgcatagcctttggtaacttcttttcttacactttcttggtgtacatacaagacaacgtcggttggtcgcttggctatgggctcccaacaatagggcttgtgatctcagtgttggtgttcttggttggtaccccattttacaggcacaaaatgccttcagggagtcctataacaagggttgctcaggtactggttgcagctgttaggaagtggaaagtgaataacccaaatgaccccaaagagcttcatgatcaaccaagcttggatgaacatgcagacaaaatgaaacacagaattgaccacacctcctcattaagattccttgacagagctgcagtaaaaagtaccaggccaagcacaccatggactctttgtccggtgacccaagtagaagaaaccaagcaaatgatgaagatgattccagttttactcaccacattcattccttccattatcatagctcaaacccacacccttttcataaagcagggcaccactctagatgcttccatgggtccccatttcaaaatcccccctgcatctttcggagccttcgtaaccattttcgtgctagtaaccctcgcaatctatgaccgctacttggttccaacactacggagttacacccaaaaccctaggggaatcacattgctccaaagaatgggaatagggtatgtggtgcagatcgtaatcatggtccttgcttgcttggttgagaaaaaacgacttctcgtcgcaagggaaaacggcattgttggcaaaggagagaaagttcccctcacaatatttattctgctgcctcagtttgctcttatgggtgtggctgatgcgtttctggaagttgcaaagatagagttcttctacgaccaagcaccacaaggcatgaagagcatggggacatcatattatactagcagctatgcagttgggagtttcctcagtagttttcttctgaaaacagtttctgatatgaccaagagagatggccacaaaggttgggtcttggacaatttgaatatatcacgcttagactactactatgcattattggccggtttggtatttcttaatttcatattatttttggttgttgcaaagtcttttgcttataatgttgacatctcggaagccaagatagctgtggaagcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

623

Amino Acids

69.11

Weight (kDa)

9.0

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017245)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20840
prunus_persica Prupe.8G218300_v2.0.a1 Prupe.8G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0287231
rosa_laevigata RLG00000012516
rosa_multiflora Rmu_sc0005297.1_g000025
rosa_roxburghii Rroxscaffold_7G00181650
rosa_rugosa Rorug06G0185900 Rorug06G0186000
rosa_samantha Rh6BG302900 Rh6CG310900 Rh6DG294600
rosa_wichuraiana Rw6G025700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1830
Acc65I GGTACC 2 cut(s) 365, 848
AccB1I GGYRCC 3 cut(s) 365, 848, 1211
AccB7I CCANNNNNTGG 1 cut(s) 1381
AccI GTMKAC 1 cut(s) 190
AciI CCGC 4 cut(s) 81, 519, 605, 1315
AcoI YGGCCR 3 cut(s) 418, 1705, 1770
AcuI CTGAAG 1 cut(s) 861
AdeI CACNNNGTG 1 cut(s) 59
AfaI GTAC 6 cut(s) 367, 596, 764, 850, 909, 1066
AfiI CCNNNNNNNGG 5 cut(s) 389, 518, 812, 1321, 1381
AgsI TTSAA 3 cut(s) 342, 1246, 1735
AhdI GACNNNNNGTC 2 cut(s) 1092, 1451
AjnI CCWGG 2 cut(s) 523, 1067
AleI CACNNNNGTG 1 cut(s) 514
AloI GAACNNNNNNTCC 2 cut(s) 1476, 1508
Alw26I GTCTC 1 cut(s) 499
AlwNI CAGNNNCTG 2 cut(s) 910, 1685
AoxI GGCC 5 cut(s) 418, 520, 1070, 1705, 1770
ApeKI GCWGC 4 cut(s) 917, 1052, 1516, 1635
ArsI GACNNNNNNTTYG 2 cut(s) 1704, 1736
Asp718I GGTACC 2 cut(s) 365, 848
AspA2I CCTAGG 1 cut(s) 1355
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 4 cut(s) 429, 521, 1235, 1417
AsuHPI GGTGA 3 cut(s) 343, 1111, 1144
AvaII GGWCC 3 cut(s) 429, 1235, 1417
AvrII CCTAGG 1 cut(s) 1355
BaeGI GKGCMC 1 cut(s) 1214
BalI TGGCCA 1 cut(s) 1707
BanI GGYRCC 3 cut(s) 365, 848, 1211
BanII GRGCYC 1 cut(s) 804
BbvI GCAGC 4 cut(s) 929, 1039, 1503, 1647
BccI CCATC 7 cut(s) 29, 40, 161, 213, 259, 520, 1697
BceAI ACGGC 1 cut(s) 1483
BciT130I CCWGG 2 cut(s) 525, 1069
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 1 cut(s) 499
BfmI CTRYAG 2 cut(s) 174, 1053
BfoI RGCGCY 1 cut(s) 396
BisI GCNGC 5 cut(s) 520, 918, 1053, 1517, 1636
BlnI CCTAGG 1 cut(s) 1355
BlsI GCNGC 5 cut(s) 521, 919, 1054, 1518, 1637
Bme1390I CCNGG 2 cut(s) 525, 1069
Bme18I GGWCC 3 cut(s) 429, 1235, 1417
BmeRI GACNNNNNGTC 2 cut(s) 1092, 1451
BmgT120I GGNCC 4 cut(s) 429, 521, 1235, 1417
BmiI GGNNCC 8 cut(s) 367, 803, 850, 1213, 1236, 1237, 1270, 1326
BmrFI CCNGG 2 cut(s) 525, 1069
BmrI ACTGGG 2 cut(s) 23, 222
BmsI GCATC 5 cut(s) 452, 694, 1213, 1268, 1537
BmuI ACTGGG 2 cut(s) 23, 222
Bpu10I CCTNAGC 1 cut(s) 903
BpuEI CTTGAG 1 cut(s) 155
BsaBI GATNNNNATC 2 cut(s) 411, 1409
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 5 cut(s) 523, 642, 1082, 1230, 1355
BsaWI WCCGGW 1 cut(s) 1096
BsaXI ACNNNNNCTCC 6 cut(s) 1010, 1040, 1260, 1290, 1476, 1506
Bsc4I CCNNNNNNNGG 5 cut(s) 389, 518, 812, 1321, 1381
Bse118I RCCGGY 1 cut(s) 1772
Bse1I ACTGG 6 cut(s) 18, 217, 362, 367, 915, 1142
Bse3DI GCAATG 1 cut(s) 1367
Bse8I GATNNNNATC 2 cut(s) 411, 1409
BseBI CCWGG 2 cut(s) 525, 1069
BseDI CCNNGG 5 cut(s) 523, 642, 1082, 1230, 1355
BseGI GGATG 4 cut(s) 23, 270, 720, 991
BseJI GATNNNNATC 2 cut(s) 411, 1409
BseLI CCNNNNNNNGG 5 cut(s) 389, 518, 812, 1321, 1381
BseMI GCAATG 1 cut(s) 1367
BseMII CTCAG 5 cut(s) 494, 840, 917, 1535, 1672
BseNI ACTGG 6 cut(s) 18, 217, 362, 367, 915, 1142
BseRI GAGGAG 1 cut(s) 1018
BseSI GKGCMC 1 cut(s) 1214
BseXI GCAGC 4 cut(s) 929, 1039, 1503, 1647
BsgI GTGCAG 2 cut(s) 529, 1421
BshFI GGCC 5 cut(s) 420, 522, 1072, 1707, 1772
BshNI GGYRCC 3 cut(s) 365, 848, 1211
BsiSI CCGG 2 cut(s) 1097, 1773
BslFI GGGAC 4 cut(s) 64, 184, 1221, 1630
BslI CCNNNNNNNGG 5 cut(s) 389, 518, 812, 1321, 1381
BsmAI GTCTC 1 cut(s) 499
BsmFI GGGAC 4 cut(s) 64, 184, 1221, 1630
BsmI GAATGC 1 cut(s) 145
BsnI GGCC 5 cut(s) 420, 522, 1072, 1707, 1772
Bso31I GGTCTC 1 cut(s) 499
Bsp1286I GDGCHC 2 cut(s) 804, 1214
Bsp1407I TGTACA 2 cut(s) 594, 762
Bsp143I GATC 4 cut(s) 203, 822, 970, 1404
Bsp19I CCATGG 3 cut(s) 642, 1082, 1230
BspACI CCGC 4 cut(s) 81, 519, 605, 1315
BspANI GGCC 5 cut(s) 420, 522, 1072, 1707, 1772
BspCNI CTCAG 5 cut(s) 495, 839, 916, 1534, 1671
BspHI TCATGA 1 cut(s) 967
BspLI GGNNCC 8 cut(s) 367, 803, 850, 1213, 1236, 1237, 1270, 1326
BspMAI CTGCAG 1 cut(s) 1057
BspQI GCTCTTC 2 cut(s) 34, 1601
BspT107I GGYRCC 3 cut(s) 365, 848, 1211
BspTNI GGTCTC 1 cut(s) 499
BsrDI GCAATG 1 cut(s) 1367
BsrFI RCCGGY 1 cut(s) 1772
BsrGI TGTACA 2 cut(s) 594, 762
BsrI ACTGG 6 cut(s) 18, 217, 362, 367, 915, 1142
BssAI RCCGGY 1 cut(s) 1772
BssECI CCNNGG 5 cut(s) 523, 642, 1082, 1230, 1355
BssMI GATC 4 cut(s) 203, 822, 970, 1404
BssT1I CCWWGG 4 cut(s) 642, 1082, 1230, 1355
Bst2UI CCWGG 2 cut(s) 525, 1069
Bst4CI ACNGT 3 cut(s) 331, 665, 1681
Bst6I CTCTTC 2 cut(s) 34, 1601
BstAPI GCANNNNNTGC 1 cut(s) 871
BstAUI TGTACA 2 cut(s) 594, 762
BstC8I GCNNGC 3 cut(s) 229, 498, 1426
BstDEI CTNAG 6 cut(s) 503, 826, 903, 1521, 1658, 1747
BstDSI CCRYGG 3 cut(s) 642, 1082, 1230
BstEII GGTNACC 1 cut(s) 1099
BstF5I GGATG 4 cut(s) 23, 270, 720, 991
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 4 cut(s) 206, 825, 973, 1407
BstMAI GTCTC 1 cut(s) 499
BstMBI GATC 4 cut(s) 203, 822, 970, 1404
BstMWI GCNNNNNNNGC 8 cut(s) 401, 691, 871, 1476, 1608, 1769, 1856, 1865
BstNI CCWGG 2 cut(s) 525, 1069
BstNSI RCATGY 1 cut(s) 995
BstPI GGTNACC 1 cut(s) 1099
BstSCI CCNGG 2 cut(s) 523, 1067
BstSFI CTRYAG 2 cut(s) 174, 1053
BstSLI GKGCMC 1 cut(s) 1214
BstV1I GCAGC 4 cut(s) 929, 1039, 1503, 1647
BstXI CCANNNNNNTGG 1 cut(s) 109
BsuRI GGCC 5 cut(s) 420, 522, 1072, 1707, 1772
BtgI CCRYGG 3 cut(s) 642, 1082, 1230
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 4 cut(s) 23, 270, 720, 991
BtsIMutI CAGTG 3 cut(s) 327, 498, 834
Cac8I GCNNGC 3 cut(s) 229, 498, 1426
CaiI CAGNNNCTG 2 cut(s) 910, 1685
CciI TCATGA 1 cut(s) 967
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 1772
Cfr13I GGNCC 4 cut(s) 429, 521, 1235, 1417
Csp6I GTAC 6 cut(s) 366, 595, 763, 849, 908, 1065
CspCI CAANNNNNGTGG 4 cut(s) 276, 311, 1697, 1732
CviQI GTAC 6 cut(s) 366, 595, 763, 849, 908, 1065
DdeI CTNAG 6 cut(s) 503, 826, 903, 1521, 1658, 1747
DpnI GATC 4 cut(s) 205, 824, 972, 1406
DpnII GATC 4 cut(s) 203, 822, 970, 1404
DraIII CACNNNGTG 1 cut(s) 59
DriI GACNNNNNGTC 2 cut(s) 1092, 1451
EaeI YGGCCR 3 cut(s) 418, 1705, 1770
Eam1104I CTCTTC 2 cut(s) 34, 1601
Eam1105I GACNNNNNGTC 2 cut(s) 1092, 1451
EarI CTCTTC 2 cut(s) 34, 1601
Eco130I CCWWGG 4 cut(s) 642, 1082, 1230, 1355
Eco24I GRGCYC 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 499
Eco47I GGWCC 3 cut(s) 429, 1235, 1417
Eco57I CTGAAG 1 cut(s) 861
Eco91I GGTNACC 1 cut(s) 1099
EcoO109I RGGNCCY 1 cut(s) 1235
EcoO65I GGTNACC 1 cut(s) 1099
EcoRII CCWGG 2 cut(s) 523, 1067
EcoT14I CCWWGG 4 cut(s) 642, 1082, 1230, 1355
EcoT22I ATGCAT 1 cut(s) 1765
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 4 cut(s) 642, 1082, 1230, 1355
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FaqI GGGAC 4 cut(s) 64, 184, 1221, 1630
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 1 cut(s) 190
Fnu4HI GCNGC 5 cut(s) 520, 918, 1053, 1517, 1636
FokI GGATG 4 cut(s) 30, 277, 727, 998
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 5 cut(s) 520, 918, 1053, 1517, 1636
GlaI GCGC 1 cut(s) 394
GluI GCNGC 5 cut(s) 520, 918, 1053, 1517, 1636
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 5 cut(s) 420, 522, 1072, 1707, 1772
HapII CCGG 2 cut(s) 1097, 1773
HhaI GCGC 1 cut(s) 395
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 1 cut(s) 1837
HindII GTYRAC 1 cut(s) 1837
HindIII AAGCTT 3 cut(s) 692, 979, 1866
HinfI GANTC 6 cut(s) 528, 883, 1038, 1087, 1138, 1362
HpaII CCGG 2 cut(s) 1097, 1773
HphI GGTGA 3 cut(s) 343, 1111, 1144
Hpy166II GTNNAC 3 cut(s) 191, 763, 1837
Hpy188I TCNGA 6 cut(s) 504, 690, 1268, 1674, 1687, 1846
Hpy188III TCNNGA 3 cut(s) 968, 1220, 1556
Hpy8I GTNNAC 3 cut(s) 191, 763, 1837
Hpy99I CGWCG 2 cut(s) 782, 1457
HpyAV CCTTC 6 cut(s) 244, 317, 709, 885, 1176, 1282
HpyCH4III ACNGT 3 cut(s) 331, 665, 1681
HpyCH4IV ACGT 1 cut(s) 777
HpyF10VI GCNNNNNNNGC 8 cut(s) 401, 691, 871, 1476, 1608, 1769, 1856, 1865
HpyF3I CTNAG 6 cut(s) 503, 826, 903, 1521, 1658, 1747
HpySE526I ACGT 1 cut(s) 777
HspAI GCGC 1 cut(s) 393
KflI GGGWCCC 1 cut(s) 1235
KpnI GGTACC 2 cut(s) 369, 852
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 4 cut(s) 203, 822, 970, 1404
LguI GCTCTTC 2 cut(s) 34, 1601
LmnI GCTCC 6 cut(s) 104, 236, 321, 807, 1268, 1377
Lsp1109I GCAGC 4 cut(s) 929, 1039, 1503, 1647
LweI GCATC 5 cut(s) 452, 694, 1213, 1268, 1537
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 7 cut(s) 349, 468, 734, 1099, 1276, 1294, 1340
MalI GATC 4 cut(s) 205, 824, 972, 1406
MboI GATC 4 cut(s) 203, 822, 970, 1404
MhlI GDGCHC 2 cut(s) 804, 1214
MlsI TGGCCA 1 cut(s) 1707
MluCI AATT 8 cut(s) 25, 105, 612, 656, 706, 1014, 1729, 1789
MluNI TGGCCA 1 cut(s) 1707
MlyI GAGTC 2 cut(s) 892, 1081
MmeI TCCRAC 2 cut(s) 589, 1352
Mox20I TGGCCA 1 cut(s) 1707
Mph1103I ATGCAT 1 cut(s) 1765
MscI TGGCCA 1 cut(s) 1707
MseI TTAA 4 cut(s) 551, 705, 1034, 1788
MslI CAYNNNNRTG 2 cut(s) 514, 1286
Msp20I TGGCCA 1 cut(s) 1707
MspA1I CMGCKG 1 cut(s) 920
MspI CCGG 2 cut(s) 1097, 1773
MspR9I CCNGG 2 cut(s) 525, 1069
Mva1269I GAATGC 1 cut(s) 145
MvaI CCWGG 2 cut(s) 525, 1069
MwoI GCNNNNNNNGC 8 cut(s) 401, 691, 871, 1476, 1608, 1769, 1856, 1865
NcoI CCATGG 3 cut(s) 642, 1082, 1230
NdeII GATC 4 cut(s) 203, 822, 970, 1404
NlaIV GGNNCC 8 cut(s) 367, 803, 850, 1213, 1236, 1237, 1270, 1326
NmuCI GTSAC 3 cut(s) 349, 468, 1099
NsiI ATGCAT 1 cut(s) 1765
NspI RCATGY 1 cut(s) 995
OliI CACNNNNGTG 1 cut(s) 514
PagI TCATGA 1 cut(s) 967
PciSI GCTCTTC 2 cut(s) 34, 1601
PcsI WCGNNNNNNNCGW 1 cut(s) 1272
PctI GAATGC 1 cut(s) 145
PfeI GAWTC 4 cut(s) 528, 1038, 1138, 1362
PflFI GACNNNGTC 1 cut(s) 776
PflMI CCANNNNNTGG 1 cut(s) 1381
PkrI GCNGC 5 cut(s) 521, 919, 1054, 1518, 1637
PleI GAGTC 2 cut(s) 891, 1081
PpsI GAGTC 2 cut(s) 891, 1081
PpuMI RGGWCCY 1 cut(s) 1235
PsiI TTATAA 1 cut(s) 1830
Psp5II RGGWCCY 1 cut(s) 1235
Psp6I CCWGG 2 cut(s) 523, 1067
PspEI GGTNACC 1 cut(s) 1099
PspGI CCWGG 2 cut(s) 523, 1067
PspN4I GGNNCC 8 cut(s) 367, 803, 850, 1213, 1236, 1237, 1270, 1326
PspPI GGNCC 4 cut(s) 429, 521, 1235, 1417
PspPPI RGGWCCY 1 cut(s) 1235
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 2 cut(s) 910, 1685
PsyI GACNNNGTC 1 cut(s) 776
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 6 cut(s) 367, 596, 764, 850, 909, 1066
RsaNI GTAC 6 cut(s) 366, 595, 763, 849, 908, 1065
RseI CAYNNNNRTG 2 cut(s) 514, 1286
SapI GCTCTTC 2 cut(s) 34, 1601
SaqAI TTAA 4 cut(s) 551, 705, 1034, 1788
SatI GCNGC 5 cut(s) 520, 918, 1053, 1517, 1636
Sau3AI GATC 4 cut(s) 203, 822, 970, 1404
Sau96I GGNCC 4 cut(s) 429, 521, 1235, 1417
SchI GAGTC 2 cut(s) 892, 1081
ScrFI CCNGG 2 cut(s) 525, 1069
SduI GDGCHC 2 cut(s) 804, 1214
SfaNI GCATC 5 cut(s) 452, 694, 1213, 1268, 1537
SfcI CTRYAG 2 cut(s) 174, 1053
SinI GGWCC 3 cut(s) 429, 1235, 1417
SmiMI CAYNNNNRTG 2 cut(s) 514, 1286
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 8 cut(s) 25, 105, 612, 656, 706, 1014, 1729, 1789
SsiI CCGC 4 cut(s) 81, 519, 605, 1315
SspI AATATT 2 cut(s) 255, 1506
StyD4I CCNGG 2 cut(s) 523, 1067
StyI CCWWGG 4 cut(s) 642, 1082, 1230, 1355
TaaI ACNGT 3 cut(s) 331, 665, 1681
TaiI ACGT 1 cut(s) 780
TasI AATT 8 cut(s) 25, 105, 612, 656, 706, 1014, 1729, 1789
TatI WGTACW 2 cut(s) 594, 762
TauI GCSGC 1 cut(s) 522
TfiI GAWTC 4 cut(s) 528, 1038, 1138, 1362
Tru1I TTAA 4 cut(s) 551, 705, 1034, 1788
Tru9I TTAA 4 cut(s) 551, 705, 1034, 1788
TscAI CASTG 3 cut(s) 334, 498, 834
TseFI GTSAC 3 cut(s) 349, 468, 1099
TseI GCWGC 4 cut(s) 917, 1052, 1516, 1635
Tsp45I GTSAC 3 cut(s) 349, 468, 1099
TspGWI ACGGA 1 cut(s) 1351
TspRI CASTG 3 cut(s) 334, 498, 834
Tth111I GACNNNGTC 1 cut(s) 776
Van91I CCANNNNNTGG 1 cut(s) 1381
VpaK11BI GGWCC 3 cut(s) 429, 1235, 1417
XbaI TCTAGA 1 cut(s) 1219
XceI RCATGY 1 cut(s) 995
XcmI CCANNNNNNNNNTGG 3 cut(s) 457, 1080, 1858
XmaJI CCTAGG 1 cut(s) 1355
XmiI GTMKAC 1 cut(s) 190
Zsp2I ATGCAT 1 cut(s) 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.