Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005420.1

Orthogroup ID

OG0011450

Physical Location & Seq

Genomic Coordinates Forward (+)

28893 .. 42278

Gene Structure 13386 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4734 bp

atggcggtctccgaggaggagtgttcctccgccaactccagatcatcctcgtcctccacctccagcacctctcactacctctccaagtgcctcctccgtggcaccgtcgttctccacgtcctccacagtcacatccgcttccccaactcccgcgacatcgttttcggcaaggagaaatcgatagagttggtgataatcggtgaagatgggatcgtacagtctgtgtgtgagcagcctgtgttcggcaccattaaagacattgccgtcctaccttggaatgacggattccgaacccgaaaccgccagatgccgggcaaggatcttttggtcgttgtttcggattccgggaagctctcggttctttctttctgcaatgaaatgcacaggttttttcctgtgacacaagttcaactttctaatcctggaaacgcgaggaatcagcttgcaagaatgctggccattgattcaagtggctgcttcattgctgctagtgcatacgtagaccgtttggctatgttctctgtttcaatgtctggaggcagtgacattattgataagaaaatagtgtatccccctgaaaatgaagtcaatgtgaatgctgctagagtccagaaaaacagtatatcaggtactatatggagcatgagctttatttcacaggatcctaatcaaagtaagggacataatcctatactcgccgttgttataaataggactggggcagtccgtaatgaattgctgttactgggatggaacattagagaacaagatgtttatgttatttctcagtatgtagaagatgatggacctttagtacttagcattgctgaagttcctcattcgtatggatttgcttttctgtttagggagggtgatgctattttaatggatcttagagatgccaacaacccttattgtatctacagaacaagcccaaatttcttatccaatgtggttgatgaggcaaattttgttcaggagtcgtctaaaggatgtgatctgtctagcgtactacaggttgatgacgaagggggtttatttaatgtagctgcctgtgcgttactggaactgagtgatttagatcctatgtgtatagatggtgacaaatacaatgtgaatgtgactcacaaatttgtgtgttcatggagttgggaaccagggaatgttaaaaaccaaagaatgatcatttctgctgataccggagaatatttcatgattgaaatttttttcaaccctgatggcgttaaggttcaagaatctgagtgtctttacaaaggtttaccatgcaaggcaattttgtgggttgaaggcgggtttctggctgcactagtagatatgggggatgggatggtcttgaaaatggaaaacggaacacttcattacagaagtcctattcaaaccattgtcccagtcttggatatgtcagttgtggattaccatgatgagaaacatgatcaaatgtttgcttgttgtggagtggcaccggaggggtcactaaggatcattagaagtggtatcactgtagaaaagctactaaggactgctcctatatatcaagggattactggcacttggacattacgaatgaaggtgattgatacttaccattcttttcttgtgctatcatttgttgaggaaaccagggtactctcagttggtttaagctttactgatgtgactgattcagttggtttccagcctgatgtttctactttggcatgtggtgttgtaaatgatggaatgctggtccagattcacagaagtgcagtcaggctctgtctgcccacccagggtgcgcattctgatggtgttcctttgccttctccagtttgtacctcttggttcccagaaaatttgagcatcagtttgggtgctgttgggcataatctaattgttgtttcttcatctaatccctgctttatcttcatacttggggtcagaatgctttcagtgcatcattatgagatatatgagatgcaacatttgagattgcaaaatgaattatcatgcatctccattcctcagaaccgttacgagaaaaaagatacaagtttttcaaacagtctggtagatgagagttctgcccctgctcttccatttggagttgacatcagtaatatctttgttattggaacacacaagccttcagtggaaatcttgtctcttgtacctagtgaaggcctacgagttcttgcttctggggcaatttcattaacaaatacttttggtactgctattagtggctgcattcctcaagatgtaaggcttgtattagttgaccgattatatgtcctttctggattgaggaatggaatgctgcttcggtttgaatggcctacttcctctcgaaggcctttctctgtaggacctcagagtcctgttgattggctttcagttagtacagatactgttttatccagtgtatcagcagcaaattcttttggtcgacatatgtatactactaagttatctgagaatataaaggacaactttcctgttgatttgcaattgattgctacccgtcggattggcatcactcctgtattcctggttcctttaagtgattcccttgatggtgatgtagttgttctcagtgataggccttggttgctgcatactgcaaggcacagcctctcatatacttctatctcatttcaatcttcgactcatgtgactcctgtatgttatgtcgaatgccccaagggaattttatttgttgcagagaactgtctacatttggtagagatgttgcacagtaagaggcttaatgtgcagaagttgcatcttggaggcactccgaggagggtcttttatcatagtgaaagcaggctattgcttgtgatgaggactaatttgagtgatgatacatgttcatctgatatatgttgcgtagatcctcttagtgggtcggtgctgtcatctttcaaacttgaatttggtgaaactggaaaatcgatggagttaatgagggttggaagtgaacaggttattttggttggaacaagtttgtcttctgggtcggccataatgccctgtggtgaggctgaaagtaccaagggacgtcttattgttctctgtcttgaaaatatgcaaaattcagacagtggttcaatgacattcggctcaaaggcagggtcatcttctcaacgagcttccccatttcatgaaattgttgggtatgccacagaacagctttcaagtagtagtctctgtagtagcccagatgatactagctgtgatggtatgaaacttgaagaaactgaatcgtggcagtttcgattagctttttcaatgccatggcctggaatggtacttgcaatctgcccttatcttgatcgttacttcttggcttctgctggtaatgctttttatttatgtggttttccacatgacaattcccaaagagtaaaaaagtgggctgtggcgaggacgcgctttacaattacatctttgactgcatatttcactagaattgttgttggtgattgtcgtgatggcatccttttctatgattatcatgaggagtccaaaaagctgcagcaactttactgtgatccgtatcagagattagttggtgattgcattcttatggatgtcaacactgctgttgtttcagatcgtaagggaagcattgctgtcctttcatgtgcagattatttagaaggtaaagtacggaggcgttccaggcggtgtgcggcttgcaggagggaggccggagacatctcggactgttctgggcagcgttcaaggtcggggttgcaggttctgggcagcaacccgacctgcaactgcaggttttctgggcagcgctgcaacgacgttgccggctactccaccgattgcagaccgctctgcccgacccctgaagtccttccggcaacccccgccgctccgtcgcgccccgagccgagctgcagcagctccgtccgcagtttagcgaaagtgcggacgtcctctttggaacgcctccgtgtgtcaggcggcttccaggcgtcgatggcggcgcgggaggccggaggcttcccggaccgttctaggcaccgttcgaggtcgggcttgccggtttttgggcagccacctcacctgcaactgaaggttctgtgcagcaccgcaaaaccagtcgccggcgactccaccagaccgcagacccctccgcacgacccctgcctccctccggcaagccgcgccgtcgccgctagatcgaggccggagcagcgacatactgcaagtccagaatgtaatttaacagtgagctgtgcttattatatgggtgaggtagctatgagcatcaagaagggctcattttcatataaacttccagctgatgatgcattgaaaggcagtgatggaagtattgacttctctcaaaacggtatcattgtgagtacactgctaggaagcataataactttcgttccaatatcaagggaagaatatgaactcttagaagctgtccaagatagactggcggtacatccattaaccgctcctattctcggaaatgatcataatgagtttcgtagccgtgaaaatccggttggagtacccaagattcttgatgctgacatgctcactcaattcttggagcttacgagtatgcaacaagaggatgtattatcatcacctctttgcgtacaaggcacagtaaagtcacgtttgaaattgcgaagtttgcctgttcctgtcaatcaggttgtgcaactccttgagcgagttcactatgccctaaactaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010498 GO:0019538 GO:0019941 GO:0022414 GO:0030163 GO:0031461 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034641 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051603 GO:0051716 GO:0061458 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

CPSF_A MMS1_N

Protein Analysis

1577

Amino Acids

173.0

Weight (kDa)

6.04

Isoelectric Point (pI)

49.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_RSE1_1st	PF10433	37 - 467	2.2e-42	RSE1/DDB1/CPSF1 first beta-propeller
Beta-prop_RSE1_2nd	PF23726	519 - 915	3.5e-29	RSE1/DDB1/CPSF1 second beta-propeller
CPSF_A	PF03178	1090 - 1217	2.1e-11	CPSF A subunit region
CPSF_A	PF03178	1403 - 1528	2e-11	CPSF A subunit region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011450)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11960 AT3G11960 AT3G11960 AT3G11960
fragaria_vesca	FvH4_6g21460 FvH4_6g21460 FvH4_6g21460
malus_domestica	MD14G1049600.v1.1
prunus_persica	Prupe.7G085900_v2.0.a1
pyrus_communis	pycom14g04060
rosa_chinensis	RchiOBHm_Chr3g0478641
rosa_laevigata	RLG00000023638
rosa_multiflora	Rmu_sc0005420.1_g000008
rosa_roxburghii	Rroxscaffold_6G00403250
rosa_rugosa	Rorug03G0166800
rosa_samantha	Rh3AG218800 Rh3BG250400 Rh3CG247300 Rh3DG243600
rosa_wichuraiana	Rw3G019670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	707
AarI	CACCTGC	1 cut(s)	4092
AasI	GACNNNNNNGTC	2 cut(s)	263, 2373
AatII	GACGTC	2 cut(s)	3064, 3953
Acc16I	TGCGCA	1 cut(s)	1797
Acc36I	ACCTGC	4 cut(s)	3751, 3783, 3791, 4092
AccB1I	GGYRCC	4 cut(s)	101, 245, 1480, 4038
AccB7I	CCANNNNNTGG	1 cut(s)	3302
AccBSI	CCGCTC	3 cut(s)	3850, 3890, 4487
AccI	GTMKAC	4 cut(s)	501, 2446, 2456, 2731
AccII	CGCG	6 cut(s)	153, 431, 3433, 3898, 4006, 4185
AclWI	GGATC	9 cut(s)	218, 327, 656, 669, 897, 1076, 1508, 2888, 3548
AcoI	YGGCCR	2 cut(s)	456, 3021
AcsI	RAATTY	9 cut(s)	937, 967, 1130, 1221, 1852, 2434, 2706, 2933, 3094
AcuI	CTGAAG	4 cut(s)	849, 2130, 3885, 4112
AcyI	GRCGYC	3 cut(s)	3061, 3950, 3992
AfeI	AGCGCT	1 cut(s)	3809
AflIII	ACRYGT	1 cut(s)	2867
AhlI	ACTAGT	1 cut(s)	1327
AjiI	CACGTC	1 cut(s)	118
AjnI	CCWGG	8 cut(s)	421, 1156, 1640, 1788, 2547, 3301, 3683, 3987
AjuI	GAANNNNNNNTTGG	2 cut(s)	4521, 4553
AleI	CACNNNNGTG	1 cut(s)	1761
Alw26I	GTCTC	4 cut(s)	13, 2166, 3212, 3711
AlwI	GGATC	9 cut(s)	218, 327, 656, 669, 897, 1076, 1508, 2888, 3548
AlwNI	CAGNNNCTG	3 cut(s)	1776, 2408, 3766
Ama87I	CYCGRG	1 cut(s)	3902
Aor51HI	AGCGCT	1 cut(s)	3809
ApoI	RAATTY	9 cut(s)	937, 967, 1130, 1221, 1852, 2434, 2706, 2933, 3094
ArsI	GACNNNNNNTTYG	6 cut(s)	146, 178, 3119, 3151, 3940, 3972
Asp700I	GAANNNNTTC	2 cut(s)	1945, 3870
AspLEI	GCGC	6 cut(s)	1798, 3435, 3810, 3900, 4006, 4187
AspS9I	GGNCC	4 cut(s)	806, 1747, 2366, 4027
AsuC2I	CCSGG	3 cut(s)	312, 346, 4025
AvaI	CYCGRG	1 cut(s)	3902
AvaII	GGWCC	4 cut(s)	806, 1747, 2366, 4027
BalI	TGGCCA	1 cut(s)	458
BamHI	GGATCC	1 cut(s)	661
BanI	GGYRCC	4 cut(s)	101, 245, 1480, 4038
BanII	GRGCYC	1 cut(s)	4301
BarI	GAAGNNNNNNTAC	2 cut(s)	3654, 3686
BbsI	GAAGAC	1 cut(s)	3003
BceAI	ACGGC	4 cut(s)	248, 683, 4172, 4509
BciT130I	CCWGG	8 cut(s)	423, 1158, 1642, 1790, 2549, 3303, 3685, 3989
BciVI	GTATCC	1 cut(s)	579
BclI	TGATCA	3 cut(s)	1182, 1453, 4504
BcnI	CCSGG	3 cut(s)	312, 346, 4025
BcoDI	GTCTC	4 cut(s)	13, 2166, 3212, 3711
BcuI	ACTAGT	1 cut(s)	1327
BfmI	CTRYAG	8 cut(s)	922, 1013, 1521, 2361, 3211, 3536, 3790, 3913
BfoI	RGCGCY	1 cut(s)	3811
BfuAI	ACCTGC	4 cut(s)	3751, 3783, 3791, 4092
BfuI	GTATCC	1 cut(s)	579
BmcAI	AGTACT	1 cut(s)	816
Bme18I	GGWCC	4 cut(s)	806, 1747, 2366, 4027
BmeT110I	CYCGRG	1 cut(s)	3902
BmgBI	CACGTC	1 cut(s)	118
BmgT120I	GGNCC	4 cut(s)	806, 1747, 2366, 4027
BmiI	GGNNCC	8 cut(s)	103, 247, 663, 1155, 1482, 1844, 2553, 4040
BmrI	ACTGGG	3 cut(s)	726, 755, 1403
BmuI	ACTGGG	3 cut(s)	726, 755, 1403
BpiI	GAAGAC	1 cut(s)	3003
BplI	GAGNNNNNCTC	2 cut(s)	11, 43
BpmI	CTGGAG	4 cut(s)	22, 46, 555, 1809
BpuEI	CTTGAG	2 cut(s)	2238, 4727
BpuMI	CCSGG	3 cut(s)	312, 346, 4025
Bsa29I	ATCGAT	2 cut(s)	179, 2954
BsaAI	YACGTR	1 cut(s)	499
BsaBI	GATNNNNATC	4 cut(s)	666, 1080, 2531, 3558
BsaHI	GRCGYC	3 cut(s)	3061, 3950, 3992
BsaI	GGTCTC	1 cut(s)	13
BsaWI	WCCGGW	3 cut(s)	1199, 1483, 4534
BsaXI	ACNNNNNCTCC	2 cut(s)	65, 95
Bse118I	RCCGGY	3 cut(s)	3824, 4060, 4124
Bse3DI	GCAATG	5 cut(s)	258, 379, 480, 822, 3630
Bse8I	GATNNNNATC	4 cut(s)	666, 1080, 2531, 3558
BseBI	CCWGG	8 cut(s)	423, 1158, 1642, 1790, 2549, 3303, 3685, 3989
BseCI	ATCGAT	2 cut(s)	179, 2954
BseJI	GATNNNNATC	4 cut(s)	666, 1080, 2531, 3558
BseMI	GCAATG	5 cut(s)	258, 379, 480, 822, 3630
BseMII	CTCAG	8 cut(s)	800, 1061, 1251, 1665, 2036, 2384, 2463, 2605
BseRI	GAGGAG	5 cut(s)	29, 32, 83, 2815, 3536
BsgI	GTGCAG	5 cut(s)	1308, 1785, 2792, 3669, 4123
Bsh1236I	CGCG	6 cut(s)	153, 431, 3433, 3898, 4006, 4185
BshNI	GGYRCC	4 cut(s)	101, 245, 1480, 4038
BshVI	ATCGAT	2 cut(s)	179, 2954
BsiHKCI	CYCGRG	1 cut(s)	3902
BslFI	GGGAC	3 cut(s)	693, 1391, 3072
BsmAI	GTCTC	4 cut(s)	13, 2166, 3212, 3711
BsmFI	GGGAC	3 cut(s)	693, 1391, 3072
BsmI	GAATGC	9 cut(s)	456, 601, 1746, 1798, 1947, 2247, 2319, 2699, 3582
Bso31I	GGTCTC	1 cut(s)	13
BsoBI	CYCGRG	1 cut(s)	3902
Bsp1286I	GDGCHC	1 cut(s)	4301
Bsp19I	CCATGG	1 cut(s)	3296
BspCNI	CTCAG	8 cut(s)	799, 1062, 1252, 1664, 2035, 2383, 2464, 2604
BspDI	ATCGAT	2 cut(s)	179, 2954
BspFNI	CGCG	6 cut(s)	153, 431, 3433, 3898, 4006, 4185
BspHI	TCATGA	3 cut(s)	1212, 3163, 3517
BspLI	GGNNCC	8 cut(s)	103, 247, 663, 1155, 1482, 1844, 2553, 4040
BspMAI	CTGCAG	3 cut(s)	3540, 3794, 3917
BspMI	ACCTGC	4 cut(s)	3751, 3783, 3791, 4092
BspPI	GGATC	9 cut(s)	218, 327, 656, 669, 897, 1076, 1508, 2888, 3548
BspQI	GCTCTTC	1 cut(s)	2097
BspT107I	GGYRCC	4 cut(s)	101, 245, 1480, 4038
BspTNI	GGTCTC	1 cut(s)	13
BsrBI	CCGCTC	3 cut(s)	3850, 3890, 4487
BsrDI	GCAATG	5 cut(s)	258, 379, 480, 822, 3630
BsrFI	RCCGGY	3 cut(s)	3824, 4060, 4124
BssAI	RCCGGY	3 cut(s)	3824, 4060, 4124
BssNAI	GTATAC	1 cut(s)	2457
BssNI	GRCGYC	3 cut(s)	3061, 3950, 3992
BssT1I	CCWWGG	5 cut(s)	272, 2603, 2700, 3054, 3296
Bst1107I	GTATAC	1 cut(s)	2457
Bst2UI	CCWGG	8 cut(s)	423, 1158, 1642, 1790, 2549, 3303, 3685, 3989
Bst6I	CTCTTC	1 cut(s)	2097
BstACI	GRCGYC	3 cut(s)	3061, 3950, 3992
BstAPI	GCANNNNNTGC	1 cut(s)	2779
BstBAI	YACGTR	1 cut(s)	499
BstC8I	GCNNGC	8 cut(s)	444, 456, 2828, 3700, 3826, 4058, 4126, 4180
BstDSI	CCRYGG	2 cut(s)	97, 3296
BstENI	CCTNNNNNAGG	2 cut(s)	2175, 2347
BstFNI	CGCG	6 cut(s)	153, 431, 3433, 3898, 4006, 4185
BstH2I	RGCGCY	1 cut(s)	3811
BstHHI	GCGC	6 cut(s)	1798, 3435, 3810, 3900, 4006, 4187
BstMAI	GTCTC	4 cut(s)	13, 2166, 3212, 3711
BstNI	CCWGG	8 cut(s)	423, 1158, 1642, 1790, 2549, 3303, 3685, 3989
BstNSI	RCATGY	3 cut(s)	1722, 2871, 4570
BstSFI	CTRYAG	8 cut(s)	922, 1013, 1521, 2361, 3211, 3536, 3790, 3913
BstSNI	TACGTA	1 cut(s)	499
BstUI	CGCG	6 cut(s)	153, 431, 3433, 3898, 4006, 4185
BstV2I	GAAGAC	1 cut(s)	3003
BstX2I	RGATCY	5 cut(s)	319, 661, 889, 1081, 2893
BstYI	RGATCY	5 cut(s)	319, 661, 889, 1081, 2893
BstZ17I	GTATAC	1 cut(s)	2457
Bsu15I	ATCGAT	2 cut(s)	179, 2954
BsuI	GTATCC	1 cut(s)	579
BsuTUI	ATCGAT	2 cut(s)	179, 2954
BtgI	CCRYGG	2 cut(s)	97, 3296
BtrI	CACGTC	1 cut(s)	118
BtsI	GCAGTG	4 cut(s)	547, 3600, 4348, 4388
BveI	ACCTGC	4 cut(s)	3751, 3783, 3791, 4092
Cac8I	GCNNGC	8 cut(s)	444, 456, 2828, 3700, 3826, 4058, 4126, 4180
CaiI	CAGNNNCTG	3 cut(s)	1776, 2408, 3766
CciI	TCATGA	3 cut(s)	1212, 3163, 3517
CfoI	GCGC	6 cut(s)	1798, 3435, 3810, 3900, 4006, 4187
Cfr10I	RCCGGY	3 cut(s)	3824, 4060, 4124
Cfr13I	GGNCC	4 cut(s)	806, 1747, 2366, 4027
ClaI	ATCGAT	2 cut(s)	179, 2954
CpoI	CGGWCCG	1 cut(s)	4027
CseI	GACGC	2 cut(s)	3439, 3981
CspI	CGGWCCG	1 cut(s)	4027
DrdI	GACNNNNNNGTC	2 cut(s)	263, 2373
DseDI	GACNNNNNNGTC	2 cut(s)	263, 2373
EaeI	YGGCCR	2 cut(s)	456, 3021
Eam1104I	CTCTTC	1 cut(s)	2097
EarI	CTCTTC	1 cut(s)	2097
EciI	GGCGGA	1 cut(s)	19
Eco105I	TACGTA	1 cut(s)	499
Eco130I	CCWWGG	5 cut(s)	272, 2603, 2700, 3054, 3296
Eco147I	AGGCCT	3 cut(s)	2181, 2353, 2602
Eco24I	GRGCYC	1 cut(s)	4301
Eco31I	GGTCTC	1 cut(s)	13
Eco47I	GGWCC	4 cut(s)	806, 1747, 2366, 4027
Eco47III	AGCGCT	1 cut(s)	3809
Eco57I	CTGAAG	4 cut(s)	849, 2130, 3885, 4112
Eco88I	CYCGRG	1 cut(s)	3902
EcoNI	CCTNNNNNAGG	2 cut(s)	2175, 2347
EcoO109I	RGGNCCY	1 cut(s)	2366
EcoRII	CCWGG	8 cut(s)	421, 1156, 1640, 1788, 2547, 3301, 3683, 3987
EcoT14I	CCWWGG	5 cut(s)	272, 2603, 2700, 3054, 3296
EcoT22I	ATGCAT	2 cut(s)	2012, 4333
EcoT38I	GRGCYC	1 cut(s)	4301
ErhI	CCWWGG	5 cut(s)	272, 2603, 2700, 3054, 3296
FalI	AAGNNNNNCTT	6 cut(s)	396, 428, 2474, 2506, 2995, 3027
FaqI	GGGAC	3 cut(s)	693, 1391, 3072
FauI	CCCGC	4 cut(s)	158, 1304, 3892, 3999
FauNDI	CATATG	1 cut(s)	2451
FbaI	TGATCA	3 cut(s)	1182, 1453, 4504
FblI	GTMKAC	4 cut(s)	501, 2446, 2456, 2731
FriOI	GRGCYC	1 cut(s)	4301
FspAI	RTGCGCAY	1 cut(s)	1797
FspI	TGCGCA	1 cut(s)	1797
GlaI	GCGC	6 cut(s)	1797, 3434, 3809, 3899, 4005, 4186
GsuI	CTGGAG	4 cut(s)	22, 46, 555, 1809
HaeII	RGCGCY	1 cut(s)	3811
HgaI	GACGC	2 cut(s)	3439, 3981
HhaI	GCGC	6 cut(s)	1798, 3435, 3810, 3900, 4006, 4187
Hin1I	GRCGYC	3 cut(s)	3061, 3950, 3992
Hin6I	GCGC	6 cut(s)	1796, 3433, 3808, 3898, 4004, 4185
HinP1I	GCGC	6 cut(s)	1796, 3433, 3808, 3898, 4004, 4185
HincII	GTYRAC	4 cut(s)	2107, 2278, 2447, 3598
HindII	GTYRAC	4 cut(s)	2107, 2278, 2447, 3598
HindIII	AAGCTT	1 cut(s)	1663
Hpy99I	CGWCG	6 cut(s)	110, 2526, 3821, 3898, 3997, 4193
HpyCH4IV	ACGT	6 cut(s)	117, 498, 3061, 3819, 3950, 4654
HpySE526I	ACGT	6 cut(s)	117, 498, 3061, 3819, 3950, 4654
Hsp92I	GRCGYC	3 cut(s)	3061, 3950, 3992
HspAI	GCGC	6 cut(s)	1796, 3433, 3808, 3898, 4004, 4185
KroI	GCCGGC	2 cut(s)	3824, 4124
KroNI	GCCGGC	2 cut(s)	3826, 4126
Ksp22I	TGATCA	3 cut(s)	1182, 1453, 4504
LguI	GCTCTTC	1 cut(s)	2097
LmnI	GCTCC	7 cut(s)	639, 1549, 3895, 3926, 4210, 4492, 4585
MaeII	ACGT	6 cut(s)	117, 498, 3061, 3819, 3950, 4654
MbiI	CCGCTC	3 cut(s)	3850, 3890, 4487
MfeI	CAATTG	1 cut(s)	2507
MflI	RGATCY	5 cut(s)	319, 661, 889, 1081, 2893
MhlI	GDGCHC	1 cut(s)	4301
MlsI	TGGCCA	1 cut(s)	458
MluNI	TGGCCA	1 cut(s)	458
MlyI	GAGTC	8 cut(s)	615, 989, 1117, 2383, 2659, 2668, 3533, 4124
MmeI	TCCRAC	4 cut(s)	2504, 2953, 2977, 4519
Mox20I	TGGCCA	1 cut(s)	458
Mph1103I	ATGCAT	2 cut(s)	2012, 4333
MreI	CGCCGGCG	1 cut(s)	4124
MroNI	GCCGGC	2 cut(s)	3824, 4124
MroXI	GAANNNNTTC	2 cut(s)	1945, 3870
MscI	TGGCCA	1 cut(s)	458
MslI	CAYNNNNRTG	5 cut(s)	843, 1761, 1803, 1959, 3587
Msp20I	TGGCCA	1 cut(s)	458
MspA1I	CMGCKG	1 cut(s)	4322
MunI	CAATTG	1 cut(s)	2507
Mva1269I	GAATGC	9 cut(s)	456, 601, 1746, 1798, 1947, 2247, 2319, 2699, 3582
MvaI	CCWGG	8 cut(s)	423, 1158, 1642, 1790, 2549, 3303, 3685, 3989
MvnI	CGCG	6 cut(s)	153, 431, 3433, 3898, 4006, 4185
NaeI	GCCGGC	2 cut(s)	3826, 4126
NciI	CCSGG	3 cut(s)	312, 346, 4025
NcoI	CCATGG	1 cut(s)	3296
NdeI	CATATG	1 cut(s)	2451
NgoMIV	GCCGGC	2 cut(s)	3824, 4124
NlaIV	GGNNCC	8 cut(s)	103, 247, 663, 1155, 1482, 1844, 2553, 4040
NmeAIII	GCCGAG	1 cut(s)	3933
NmuCI	GTSAC	9 cut(s)	128, 397, 542, 1100, 1120, 1491, 1675, 2671, 4650
NsbI	TGCGCA	1 cut(s)	1797
NsiI	ATGCAT	2 cut(s)	2012, 4333
NspI	RCATGY	3 cut(s)	1722, 2871, 4570
OliI	CACNNNNGTG	1 cut(s)	1761
PagI	TCATGA	3 cut(s)	1212, 3163, 3517
PaqCI	CACCTGC	1 cut(s)	4092
PasI	CCCWGGG	1 cut(s)	1789
PceI	AGGCCT	3 cut(s)	2181, 2353, 2602
PciI	ACATGT	1 cut(s)	2867
PciSI	GCTCTTC	1 cut(s)	2097
PcsI	WCGNNNNNNNCGW	1 cut(s)	114
PctI	GAATGC	9 cut(s)	456, 601, 1746, 1798, 1947, 2247, 2319, 2699, 3582
PdiI	GCCGGC	2 cut(s)	3826, 4126
PdmI	GAANNNNTTC	2 cut(s)	1945, 3870
PflMI	CCANNNNNTGG	1 cut(s)	3302
PfoI	TCCNGGA	3 cut(s)	344, 421, 4023
PleI	GAGTC	8 cut(s)	614, 988, 1117, 2382, 2659, 2668, 3532, 4124
PpsI	GAGTC	8 cut(s)	614, 988, 1117, 2382, 2659, 2668, 3532, 4124
Ppu21I	YACGTR	1 cut(s)	499
PpuMI	RGGWCCY	1 cut(s)	2366
PscI	ACATGT	1 cut(s)	2867
PsiI	TTATAA	1 cut(s)	707
Psp5II	RGGWCCY	1 cut(s)	2366
Psp6I	CCWGG	8 cut(s)	421, 1156, 1640, 1788, 2547, 3301, 3683, 3987
PspGI	CCWGG	8 cut(s)	421, 1156, 1640, 1788, 2547, 3301, 3683, 3987
PspN4I	GGNNCC	8 cut(s)	103, 247, 663, 1155, 1482, 1844, 2553, 4040
PspPI	GGNCC	4 cut(s)	806, 1747, 2366, 4027
PspPPI	RGGWCCY	1 cut(s)	2366
PsrI	GAACNNNNNNTAC	2 cut(s)	2535, 2567
PstI	CTGCAG	3 cut(s)	3540, 3794, 3917
PstNI	CAGNNNCTG	3 cut(s)	1776, 2408, 3766
PsuI	RGATCY	5 cut(s)	319, 661, 889, 1081, 2893
PvuII	CAGCTG	1 cut(s)	4322
RseI	CAYNNNNRTG	5 cut(s)	843, 1761, 1803, 1959, 3587
Rsr2I	CGGWCCG	1 cut(s)	4027
RsrII	CGGWCCG	1 cut(s)	4027
SalI	GTCGAC	1 cut(s)	2445
SapI	GCTCTTC	1 cut(s)	2097
Sau96I	GGNCC	4 cut(s)	806, 1747, 2366, 4027
ScaI	AGTACT	1 cut(s)	816
SchI	GAGTC	8 cut(s)	615, 989, 1117, 2383, 2659, 2668, 3533, 4124
SduI	GDGCHC	1 cut(s)	4301
SfcI	CTRYAG	8 cut(s)	922, 1013, 1521, 2361, 3211, 3536, 3790, 3913
SgrAI	CRCCGGYG	1 cut(s)	4124
SinI	GGWCC	4 cut(s)	806, 1747, 2366, 4027
SmiMI	CAYNNNNRTG	5 cut(s)	843, 1761, 1803, 1959, 3587
SmlI	CTYRAG	2 cut(s)	2253, 4706
SmoI	CTYRAG	2 cut(s)	2253, 4706
SnaBI	TACGTA	1 cut(s)	499
SpeI	ACTAGT	1 cut(s)	1327
SseBI	AGGCCT	3 cut(s)	2181, 2353, 2602
SspI	AATATT	1 cut(s)	1208
StuI	AGGCCT	3 cut(s)	2181, 2353, 2602
StyI	CCWWGG	5 cut(s)	272, 2603, 2700, 3054, 3296
TaiI	ACGT	6 cut(s)	120, 501, 3064, 3822, 3953, 4657
TaqII	GACCGA	1 cut(s)	2295
TatI	WGTACW	3 cut(s)	814, 2399, 4385
TauI	GCSGC	6 cut(s)	3698, 3890, 3984, 4004, 4185, 4196
TseFI	GTSAC	9 cut(s)	128, 397, 542, 1100, 1120, 1491, 1675, 2671, 4650
Tsp45I	GTSAC	9 cut(s)	128, 397, 542, 1100, 1120, 1491, 1675, 2671, 4650
TspGWI	ACGGA	9 cut(s)	86, 297, 716, 1383, 3546, 3688, 3882, 3913, 3959
Van91I	CCANNNNNTGG	1 cut(s)	3302
VpaK11BI	GGWCC	4 cut(s)	806, 1747, 2366, 4027
XagI	CCTNNNNNAGG	2 cut(s)	2175, 2347
XapI	RAATTY	9 cut(s)	937, 967, 1130, 1221, 1852, 2434, 2706, 2933, 3094
XceI	RCATGY	3 cut(s)	1722, 2871, 4570
XmiI	GTMKAC	4 cut(s)	501, 2446, 2456, 2731
XmnI	GAANNNNTTC	2 cut(s)	1945, 3870
ZraI	GACGTC	2 cut(s)	3062, 3951
ZrmI	AGTACT	1 cut(s)	816
Zsp2I	ATGCAT	2 cut(s)	2012, 4333

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.