Rmu_sc0005513.1_g000002

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005513.1
OG0015082
Physical Location & Seq
Forward (+)
19367 .. 21939
2573 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1446 bp
atgggctatgggaaggccgcttcatgcttagagttggagtctggcattggttgccaatctgattattttcagatgctggaatcgaagatattgagcgggaattggaatgattgtattcatatccttaatgccattaaggatttgacagaagaaacaagagctactgcttcgtttctagtattcaaacagtcacttttggagtattcaaagtgcggggatgacaagatggctttagacattttgcagaaagaggtttcagcattacaggtgggcagagataaggttcataacctggctcacagtatccttttctcgaaggatatggagcagggtaagatggacaacaatgaggtttgcgaattgcgaaaaaagctgttgcaagaattggaaaatttgcttcctccaccaattacactacctccgcgcaggttggaacatctggttgaaactgctgttttgacccagattgattcatgtatgtatcataatttttcggatgctgtttcactttatgcagaccatttgtgtggtagggatcagatccctactgagactgtacagattttgactgagcataaggacgaagtttggtttgtacaattttctcataatggggagtacttagcttcttcatcaagtgattgcacggccatcatatggaaggtgctggaagacggcagtgtgatattaaaacataccctatggagccaccttaaaccagtatcctttgtggcatggagccccgatgactcaaagttgcttacgtgtggaaatggtgaagtcctcaagctatgggatgtggaaacaggtacatgcaagcatacatttggagatcatgtcttcattgtcagctcatgtgcttggtttcccgactcaaagcgacttgtgtgtggcagttctgaccctgaaaaaggtatctacatgtgggattgtgatggcaacgagttaaaagcatggagaggtatgaggatgcccaagattttagatcttgctgtaacaccagatggggaaaatcttatcagcattttctctgataaagaaattcggattttgaatgtgggaacaaatgttgagcgtgttatctctgaagaccattctatcacatccctctcaatttctagagatagtgagtactttatcgtcaacctaaatagccaggagatccatatgtgggatgttgctgggaaactggagaagccactgaagtatatgggtcacaggcagagcaaatacgtaatacgctcctgcttcggtggagtgaatagtacatttattgccagcggtagtgagaactcacaggtctacatttggaatcggcgaaactctatgccaattgaggttttgtatgggcattcaaagactgtgaactgtgtgagctggaatcttaggagacctcaaatgctggcatcggctagtgatgatcataccatccatatatggggctag

Protein Analysis

481

Amino Acids

54.38

Weight (kDa)

5.51

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 178 - 307 2.6e-12 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 184 - 306 1.9e-21 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 184 - 358 2.2e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_IFT122_1st PF23381 184 - 298 5.5e-07 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 184 - 350 1.8e-20 CDC20/Fizzy WD40 domain
Beta-prop_Aladin PF25460 184 - 301 1.9e-12 Aladin seven-bladed propeller
Beta-prop_TEP1_2nd PF25047 184 - 316 4.4e-10 TEP-1 second beta-propeller
WDR55 PF24796 184 - 301 9.2e-08 WDR55
WD40 PF00400 185 - 220 1.4e-07 WD domain, G-beta repeat
EIF3I PF24805 189 - 381 1.1e-06 EIF3I
Beta-prop_WDR5 PF25175 189 - 480 1.1e-48 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 191 - 351 1.3e-11 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 192 - 438 9.1e-31 THOC3 beta-propeller domain
WD40_Gbeta PF25391 199 - 307 1.7e-08 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 244 - 395 4e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 245 - 394 4.3e-10 Prp19 WD40 domain
Beta-prop_WDR19_1st PF23389 335 - 479 2.2e-07 WDR19 first beta-propeller
Beta-prop_WDR3_1st PF25173 354 - 480 4.8e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 362 - 480 1e-11 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 421 - 480 6.9e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 421 - 480 8.4e-11 THOC3 beta-propeller domain
WD40 PF00400 444 - 480 1.6e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015082)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43920
fragaria_vesca FvH4_2g20200
malus_domestica MD10G1173700.v1.1
prunus_persica Prupe.8G212300_v2.0.a1
pyrus_communis pycom05g17030 pycom10g14980
rosa_chinensis RchiOBHm_Chr6g0285971
rosa_laevigata RLG00000012593
rosa_multiflora Rmu_sc0005513.1_g000002
rosa_roxburghii Rroxscaffold_7G00182560
rosa_rugosa Rorug06G0177800
rosa_samantha Rh6AG289900 Rh6BG291900 Rh6CG292900 Rh6DG285000
rosa_wichuraiana Rw6G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 657
AccBSI CCGCTC 1 cut(s) 96
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 1 cut(s) 424
AciI CCGC 5 cut(s) 18, 96, 213, 422, 1281
AclWI GGATC 3 cut(s) 535, 543, 1156
AcoI YGGCCR 1 cut(s) 648
AcsI RAATTY 2 cut(s) 391, 1041
AcuI CTGAAG 2 cut(s) 1107, 1223
AfaI GTAC 6 cut(s) 558, 597, 620, 811, 1133, 1267
AfiI CCNNNNNNNGG 4 cut(s) 657, 911, 1171, 1438
AflIII ACRYGT 2 cut(s) 764, 921
AgsI TTSAA 5 cut(s) 184, 207, 446, 1054, 1356
AjnI CCWGG 2 cut(s) 291, 1155
AluBI AGCT 6 cut(s) 161, 373, 626, 790, 852, 1377
AluI AGCT 6 cut(s) 161, 373, 626, 790, 852, 1377
Alw26I GTCTC 2 cut(s) 545, 1384
AlwI GGATC 3 cut(s) 535, 543, 1156
AlwNI CAGNNNCTG 1 cut(s) 76
AoxI GGCC 2 cut(s) 15, 648
ApoI RAATTY 2 cut(s) 391, 1041
AspLEI GCGC 1 cut(s) 426
AsuHPI GGTGA 1 cut(s) 788
BanII GRGCYC 1 cut(s) 743
BbsI GAAGAC 3 cut(s) 678, 832, 1095
BccI CCATC 6 cut(s) 220, 331, 659, 929, 998, 1436
BceAI ACGGC 2 cut(s) 663, 691
BciT130I CCWGG 2 cut(s) 293, 1157
BciVI GTATCC 2 cut(s) 314, 733
BclI TGATCA 1 cut(s) 1420
BcoDI GTCTC 2 cut(s) 545, 1384
BfaI CTAG 4 cut(s) 176, 1119, 1413, 1444
BfuAI ACCTGC 1 cut(s) 417
BfuI GTATCC 2 cut(s) 314, 733
BglII AGATCT 1 cut(s) 985
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
BmcAI AGTACT 2 cut(s) 620, 1133
Bme1390I CCNGG 2 cut(s) 293, 1157
BmiI GGNNCC 2 cut(s) 707, 740
BmrFI CCNGG 2 cut(s) 293, 1157
BmsI GCATC 4 cut(s) 63, 487, 960, 1415
BpiI GAAGAC 3 cut(s) 678, 832, 1095
BpmI CTGGAG 1 cut(s) 1211
BpuEI CTTGAG 1 cut(s) 770
BsaAI YACGTR 2 cut(s) 765, 1234
BsaI GGTCTC 1 cut(s) 1384
BsaXI ACNNNNNCTCC 4 cut(s) 403, 433, 822, 852
Bsc4I CCNNNNNNNGG 4 cut(s) 657, 911, 1171, 1438
Bse1I ACTGG 2 cut(s) 719, 1194
BseBI CCWGG 2 cut(s) 293, 1157
BseGI GGATG 7 cut(s) 223, 502, 802, 975, 1103, 1180, 1428
BseLI CCNNNNNNNGG 4 cut(s) 657, 911, 1171, 1438
BseMII CTCAG 2 cut(s) 540, 561
BseNI ACTGG 2 cut(s) 719, 1194
BseYI CCCAGC 1 cut(s) 1181
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 17, 650
BslI CCNNNNNNNGG 4 cut(s) 657, 911, 1171, 1438
BsmAI GTCTC 2 cut(s) 545, 1384
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 2 cut(s) 17, 650
Bso31I GGTCTC 1 cut(s) 1384
Bsp1286I GDGCHC 1 cut(s) 743
Bsp1407I TGTACA 2 cut(s) 556, 595
Bsp143I GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
BspACI CCGC 5 cut(s) 18, 96, 213, 422, 1281
BspANI GGCC 2 cut(s) 17, 650
BspCNI CTCAG 2 cut(s) 541, 562
BspFNI CGCG 1 cut(s) 424
BspLI GGNNCC 2 cut(s) 707, 740
BspMI ACCTGC 1 cut(s) 417
BspPI GGATC 3 cut(s) 535, 543, 1156
BspTNI GGTCTC 1 cut(s) 1384
BsrBI CCGCTC 1 cut(s) 96
BsrGI TGTACA 2 cut(s) 556, 595
BsrI ACTGG 2 cut(s) 719, 1194
BssMI GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
Bst2UI CCWGG 2 cut(s) 293, 1157
Bst4CI ACNGT 5 cut(s) 189, 302, 556, 1363, 1370
BstAPI GCANNNNNTGC 1 cut(s) 51
BstAUI TGTACA 2 cut(s) 556, 595
BstBAI YACGTR 2 cut(s) 765, 1234
BstC8I GCNNGC 3 cut(s) 818, 1279, 1404
BstDEI CTNAG 5 cut(s) 28, 549, 570, 622, 1385
BstENI CCTNNNNNAGG 1 cut(s) 909
BstF5I GGATG 7 cut(s) 223, 502, 802, 975, 1103, 1180, 1428
BstFNI CGCG 1 cut(s) 424
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 6 cut(s) 538, 543, 835, 988, 1164, 1423
BstMAI GTCTC 2 cut(s) 545, 1384
BstMBI GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
BstMWI GCNNNNNNNGC 2 cut(s) 51, 370
BstNI CCWGG 2 cut(s) 293, 1157
BstNSI RCATGY 2 cut(s) 816, 925
BstSCI CCNGG 2 cut(s) 291, 1155
BstSNI TACGTA 1 cut(s) 1234
BstUI CGCG 1 cut(s) 424
BstV2I GAAGAC 3 cut(s) 678, 832, 1095
BstX2I RGATCY 3 cut(s) 540, 985, 1161
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 3 cut(s) 540, 985, 1161
BsuI GTATCC 2 cut(s) 314, 733
BsuRI GGCC 2 cut(s) 17, 650
BtsCI GGATG 7 cut(s) 223, 502, 802, 975, 1103, 1180, 1428
BtsI GCAGTG 1 cut(s) 685
BtsIMutI CAGTG 2 cut(s) 685, 1199
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 3 cut(s) 818, 1279, 1404
CaiI CAGNNNCTG 1 cut(s) 76
CfoI GCGC 1 cut(s) 426
Csp6I GTAC 6 cut(s) 557, 596, 619, 810, 1132, 1266
CviAII CATG 8 cut(s) 24, 474, 735, 813, 836, 855, 922, 954
CviQI GTAC 6 cut(s) 557, 596, 619, 810, 1132, 1266
DdeI CTNAG 5 cut(s) 28, 549, 570, 622, 1385
DpnI GATC 6 cut(s) 537, 542, 834, 987, 1163, 1422
DpnII GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
EaeI YGGCCR 1 cut(s) 648
Eco105I TACGTA 1 cut(s) 1234
Eco24I GRGCYC 1 cut(s) 743
Eco31I GGTCTC 1 cut(s) 1384
Eco57I CTGAAG 2 cut(s) 1107, 1223
EcoNI CCTNNNNNAGG 1 cut(s) 909
EcoRII CCWGG 2 cut(s) 291, 1155
EcoT38I GRGCYC 1 cut(s) 743
FaeI CATG 8 cut(s) 27, 477, 738, 816, 839, 858, 925, 957
FatI CATG 8 cut(s) 23, 473, 734, 812, 835, 854, 921, 953
FauI CCCGC 2 cut(s) 89, 206
FauNDI CATATG 2 cut(s) 656, 1167
FbaI TGATCA 1 cut(s) 1420
FblI GTMKAC 1 cut(s) 1302
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 7 cut(s) 230, 509, 809, 982, 1090, 1187, 1415
FriOI GRGCYC 1 cut(s) 743
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 4 cut(s) 176, 1119, 1413, 1444
GlaI GCGC 1 cut(s) 425
GluI GCNGC 1 cut(s) 18
GsaI CCCAGC 1 cut(s) 1185
GsuI CTGGAG 1 cut(s) 1211
HaeIII GGCC 2 cut(s) 17, 650
HhaI GCGC 1 cut(s) 426
Hin1II CATG 8 cut(s) 27, 477, 738, 816, 839, 858, 925, 957
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HincII GTYRAC 1 cut(s) 1144
HindII GTYRAC 1 cut(s) 1144
HinfI GANTC 7 cut(s) 38, 80, 470, 749, 872, 1312, 1381
HphI GGTGA 1 cut(s) 788
Hpy166II GTNNAC 3 cut(s) 1144, 1303, 1366
Hpy188I TCNGA 8 cut(s) 61, 72, 496, 540, 901, 1033, 1047, 1087
Hpy188III TCNNGA 3 cut(s) 313, 869, 1119
Hpy8I GTNNAC 3 cut(s) 1144, 1303, 1366
HpyAV CCTTC 3 cut(s) 7, 310, 655
HpyCH4III ACNGT 5 cut(s) 189, 302, 556, 1363, 1370
HpyCH4IV ACGT 2 cut(s) 764, 1233
HpyCH4V TGCA 5 cut(s) 244, 379, 515, 645, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 370
HpyF3I CTNAG 5 cut(s) 28, 549, 570, 622, 1385
HpySE526I ACGT 2 cut(s) 764, 1233
Hsp92II CATG 8 cut(s) 27, 477, 738, 816, 839, 858, 925, 957
HspAI GCGC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 1420
Kzo9I GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
LmnI GCTCC 4 cut(s) 325, 705, 738, 1247
LweI GCATC 4 cut(s) 63, 487, 960, 1415
MaeI CTAG 4 cut(s) 176, 1119, 1413, 1444
MaeII ACGT 2 cut(s) 764, 1233
MaeIII GTNAC 3 cut(s) 189, 994, 1214
MalI GATC 6 cut(s) 537, 542, 834, 987, 1163, 1422
MbiI CCGCTC 1 cut(s) 96
MboI GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
MboII GAAGA 6 cut(s) 97, 161, 621, 683, 832, 1100
MfeI CAATTG 1 cut(s) 1332
MflI RGATCY 3 cut(s) 540, 985, 1161
MhlI GDGCHC 1 cut(s) 743
MlyI GAGTC 3 cut(s) 47, 743, 866
MmeI TCCRAC 2 cut(s) 15, 411
MseI TTAA 5 cut(s) 126, 135, 689, 714, 947
MslI CAYNNNNRTG 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 1281
MspR9I CCNGG 2 cut(s) 293, 1157
MunI CAATTG 1 cut(s) 1332
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 2 cut(s) 293, 1157
MvnI CGCG 1 cut(s) 424
MwoI GCNNNNNNNGC 2 cut(s) 51, 370
NdeI CATATG 2 cut(s) 656, 1167
NdeII GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
NlaIII CATG 8 cut(s) 27, 477, 738, 816, 839, 858, 925, 957
NlaIV GGNNCC 2 cut(s) 707, 740
NmuCI GTSAC 2 cut(s) 189, 1214
NspI RCATGY 2 cut(s) 816, 925
PciI ACATGT 1 cut(s) 921
PctI GAATGC 1 cut(s) 1351
PfeI GAWTC 4 cut(s) 80, 470, 1312, 1381
PflMI CCANNNNNTGG 1 cut(s) 657
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 3 cut(s) 46, 743, 866
PpsI GAGTC 3 cut(s) 46, 743, 866
Ppu21I YACGTR 2 cut(s) 765, 1234
PscI ACATGT 1 cut(s) 921
Psp6I CCWGG 2 cut(s) 291, 1155
PspFI CCCAGC 1 cut(s) 1181
PspGI CCWGG 2 cut(s) 291, 1155
PspN4I GGNNCC 2 cut(s) 707, 740
PstNI CAGNNNCTG 1 cut(s) 76
PsuI RGATCY 3 cut(s) 540, 985, 1161
RsaI GTAC 6 cut(s) 558, 597, 620, 811, 1133, 1267
RsaNI GTAC 6 cut(s) 557, 596, 619, 810, 1132, 1266
RseI CAYNNNNRTG 1 cut(s) 525
SaqAI TTAA 5 cut(s) 126, 135, 689, 714, 947
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 6 cut(s) 535, 540, 832, 985, 1161, 1420
ScaI AGTACT 2 cut(s) 620, 1133
SchI GAGTC 3 cut(s) 47, 743, 866
ScrFI CCNGG 2 cut(s) 293, 1157
SduI GDGCHC 1 cut(s) 743
SfaNI GCATC 4 cut(s) 63, 487, 960, 1415
SmiMI CAYNNNNRTG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
SnaBI TACGTA 1 cut(s) 1234
SsiI CCGC 5 cut(s) 18, 96, 213, 422, 1281
SspMI CTAG 4 cut(s) 176, 1119, 1413, 1444
StyD4I CCNGG 2 cut(s) 291, 1155
TaaI ACNGT 5 cut(s) 189, 302, 556, 1363, 1370
TaiI ACGT 2 cut(s) 767, 1236
TaqI TCGA 2 cut(s) 83, 314
TatI WGTACW 5 cut(s) 556, 595, 618, 1131, 1265
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 4 cut(s) 80, 470, 1312, 1381
Tru1I TTAA 5 cut(s) 126, 135, 689, 714, 947
Tru9I TTAA 5 cut(s) 126, 135, 689, 714, 947
TscAI CASTG 2 cut(s) 685, 1206
TseFI GTSAC 2 cut(s) 189, 1214
Tsp45I GTSAC 2 cut(s) 189, 1214
TspDTI ATGAA 6 cut(s) 12, 107, 275, 462, 621, 832
TspRI CASTG 2 cut(s) 685, 1206
Van91I CCANNNNNTGG 1 cut(s) 657
XagI CCTNNNNNAGG 1 cut(s) 909
XapI RAATTY 2 cut(s) 391, 1041
XbaI TCTAGA 1 cut(s) 1118
XceI RCATGY 2 cut(s) 816, 925
XmiI GTMKAC 1 cut(s) 1302
XspI CTAG 4 cut(s) 176, 1119, 1413, 1444
ZrmI AGTACT 2 cut(s) 620, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.