Rmu_sc0005656.1_g000015

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005656.1
OG0007863
Physical Location & Seq
Reverse (-)
64537 .. 78591
14055 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5262 bp
atgggcgtccgccacctcaccgtgcttggcgagttcaagcccttcggcttaatcgccgaagctctcgacggcaagcccgccgatgacgtcccggacaagtacgattacttccttttcgaccccgacaccgtccgagatcgcgacgagaccgacgaccacgacgttctcagctccgccttcagcgaccgctgcgaccacgagctcttcattcgaggcaatcggataatatggtcaacaggtccacgagtctgtaagagattcaccttaccttctccagtaatcaaggcttgctggtgtcgtttgggtgataggactaaagctagtctctgtgtattacaaatggcttgtttaacaatctgcaacacctcgggtgaagtagtatcaatacctgtatcagaaaccattacatcaatatggcctctaccatttggtttattactccagcaagccgctcaattgagttcaactacgtatgttccctcattatcttcaagtcctttgtttggtgcaagtccttctgtgtcttctgtaagacgagaagtattgcatacgagaagagaagttggacttagtccacagcataatttcagttttgtgagtgcaattgatcatgttgtcaaggaagaaacaagttcaatgtcctcgcatctaattctgcaagatccattggaagaaccgcaattggcttatattgaagaaagaggaaagtcaaacataatgaaggaatttgatgaaaaaactatctggacaagtgatcggattccccttatggcatcatataatacaggaaagatgcaacattctgtgtgggttgcggaaaccagtaattttaatcttgatctggcaaatgctaacttggtagatgcagttcctactgatattgtagcaaaacacttctcgtttcgtagaatatggcaagggaagggtgcgaagacagctgctagcaaggttttccttgcgactgatgacgatgcagctcctattatttgctttcttcaccaagaacaaaaaaagttgctgtctgtaaggcttcaaattgttgaaataaataatgagcttctcattgacattaaacctgacatgagctggagcataccttctgtagcagccgtacctgtaattgttacccgccctagagcaaaagtaggactgctgccatattcggacatgctcattttggcacctgaaaatgcccttcttctctattcagggaagcaatgcctgtgtaggtatatgttgccttcttgttttagtaaaggccggttttcacatagtatggagtttccggaaacatctgtttcccagggtatgaagattgtgggattggctgatgctgttgaggggcgtatcaatgttacagtaaataaggggcagatatatcggtgtgtattacgaagaagccctacatcttctctggcaaatgattgcatcacagctatggctgagggactcagttccagcttctacagccattttcttagtcttctatggaaagatggcgacttcgcttatttatcagaagctgattctagtgttcattcagaatgggattcttttcgcagcattatcatggaaatgtgtcaatcaagtaccacttctaaaatgatcgcgaacccaatacctcaggacttggagctcctgggatctctgttatgctatattgctaagtttctgggtgaggaaagctatgttgaccattacattcgtgactttcctggcctttccggaagcgttgggacatgtgaaatatccttttcacaggaatctcctccaagtttattcagatggcttgaaaactgtttgctatatggacacaactcagctaataccaatgatctaccaccattgatttgtaaagagggaaagtctgttgtaaactgggcccggaagatagtgttattctatagtttgttaaccggtgccaaagaaatagggaagaaactctcgtctggtatttactgtaatatttccacgggatcttactgcacaaatgaggaactcacagttctagcaatggttggggaaaaatttggactgcaacagcttgactcactgccttctggcatgtctcttcctttgcgacacgcattggctaagtgtcgggaatctcctcccactggttggcctgcagctgcatatgttcttctgggtcgtgaagatttggctttgtcatgtttggcacaaacatgcaattctaaggaacttgaaactgaaactaatgtgaatttcatctctatgtctgcaccttacatgctacatttgcacccggtgacaattccttctgcagtttctgatacaaatggattggacaatactaagtttgaggacacagattcgattgatgggtctatgacagatggtatggagcatatcttcaactcaagcacacagttgcgatatggtcgtgatttgcgactgaatgaggttagacggctcctgtgctctgcaagacccgtggcaattcaaacatctgttaatcccagtgcctctgatcaggacctccaacaggcccagctatggcaccttgcacaaaggactactgcccttcctcttggccgtggagcattcactctggcaaccatatatacacttctgacagaggcctttgtagtcccgaagattgtattagcaggtcgattgcctgcccagcaaaatgcaactgttaatctggacccgaatgtgaggaatgtagaagaaattaaaacttggcctgaattccacaatgctgttgctgctggactaaggctggctcctcttcagggaaagatgtcaaagacgtggatcaaatataataaaccagaggagccaaatgctgttcatgctggactactctttgctttaggattacatggttacctacgtgttttgaatattactgacatatttcagtattttcgacaggaacatgaaatcacaactctaggactaatgcttggcctggcagctacctatagagggactatgcaaccagaaatatctaagtgtctttttgttcatatacctgctcgacatccatcttcatttccagaattggagttaccaacgcttctgcagtcagcagctctaatgtcagttgggcttctttatgaaggatcagcgcacgcacaaacaatgcagattctcttgggggaaataggacgtagaactgcaggtgataatgtactagaaagggaaggttatgctgtttctgcaggattttccctgggtcttcttgcattgggtcggggagaagatgctctcggtttcatggagacagtggtcgacaaattgttccattatattggtggaaaagaagttaatgatagatctatttcatcaacactatcagtagatgacaatcgtgctgctgtgcagatgatggaagggactgcagtgaatgttgatgtcacagcacctggagccacaattgctcttgctttaatgtttttaaggaccgaatcacaggcaattgtgtctaagctctccattcctcgaacacaatttgagctgcaatatgtgaggcctgatttcattatgcttcgcgtcattgctcgaaatttgataatgtggagcaggattcatccttcccaagattgggtacagtctcagattccggatattatcaaaaatggcgttaaattccttggggatgatactgatgatattgatgaaatggatgctgaatcatttgttcaggcatatgtcaatatagtggctggagccagcatttctcttggtttgagattcgctggtacaaaggatgggcacgcacaggagttactttacaactatgctgtttactttttaaacgagatcaagccagtttctgctacaagtggaagcttcccaaagggattgtctcgctatgttgatcgggggactctggaaatatgcctgcatctcattgttctttccttgtgtgtggtaatggctggttctgggcatctacaaacctttaagctgttgagatttctccgcagcagaaattctgctgatgggcatgttaactatggcgttcagatggctgtaagcctagcaattggtttcttgttccttggtggaggaatgcagacattttcaacaagcaacagttcagttgccgctttgctgattactctttacccacgcttgccaactggaccaaatgataatagatgccatttccaggcatttcgacatttgtacgtactggcaacagaagctcgctggatccagactgttgatgttgacactggtctaccagtgtatgccccccttgaagttactataagggaaactgagcattatgcagaaacaagtttttgtgaggttacaccgtgccttctgccagagcgtgccattttgaagacactacgtatttgtggtcctcggtactggcctcaagtcatagatctcgtccctgaagataaaccttggtggacctctggggacaagaatagcccatttaattctggtgttttgtatatcaagagaaaggttggagcttgttcatatattgatgatcccgtagggtgtcagtcgttgctgtcacgtgcaatgcataaggtgtttggtttgacaagtttaaaagcacctagcccatcctctagtggccaaaatggacttggttcagttacagttgatcagttggttggtaccttctcctctgatcctagcttaattgcatttgcacaactctgttgtgacccttcctggaagagcagatctgatattgacttccaggaattttgcttgcaagtgttatttgagtgtgtgagcaaggacaggcctgctcttttacaggtctatttgtcattatacacgacaatggggtcaatggcaggtcaagtaactagtgatactgttgttttatgtgactcgttatctatctccaatcttaagcttgcactagcgtacaatgaggctttgttgagtggaatattgacctcttcgagaggtggcattgttcaatccaactttatagcatgtcttcggaagcaggtggaagcacttctagattgttctcaggacttgaaggatgatttttgtaattatatgtattctgggaagtggccaaatgcggaagaatcccagggaaataaacgttcaatacttctttcttggtaccttcaatggttttgtgtaccagtaccatctgcaattaagacagctgtggacaaagtcaagcccaagctcaagtcatcctcatctttggtcccttttctgcggttattatttccgaaagctcatgtcaatgctattgctgagatagataagctcttctcatccactaacggtagataa

Protein Analysis

1753

Amino Acids

193.44

Weight (kDa)

5.96

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC1 PF12859 31 - 274 1.2e-28 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1 PF12859 309 - 522 2.3e-31 Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN PF20518 548 - 722 6.1e-12 Anaphase-promoting complex subunit 1 middle domain
APC1_3rd PF21282 1395 - 1458 1.7e-15 APC1 beta sandwich domain
APC1_C PF18122 1535 - 1702 7.7e-33 Anaphase-promoting complex sub unit 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 3140, 4948
AasI GACNNNNNNGTC 1 cut(s) 4789
AatII GACGTC 1 cut(s) 90
Acc36I ACCTGC 5 cut(s) 2621, 3010, 3140, 4790, 4948
Acc65I GGTACC 2 cut(s) 4613, 5082
AccB1I GGYRCC 5 cut(s) 1180, 1919, 2520, 4613, 5082
AccB7I CCANNNNNTGG 1 cut(s) 2121
AccBSI CCGCTC 1 cut(s) 452
AccI GTMKAC 2 cut(s) 3261, 4216
AccII CGCG 3 cut(s) 141, 1610, 3522
AccIII TCCGGA 3 cut(s) 1285, 1724, 3592
AclI AACGTT 1 cut(s) 5062
AclWI GGATC 9 cut(s) 656, 1651, 1984, 2789, 3100, 4183, 4196, 4475, 4622
AcoI YGGCCR 3 cut(s) 2554, 4570, 5030
AcsI RAATTY 8 cut(s) 725, 2027, 2224, 2714, 3535, 3617, 3964, 4703
AcuI CTGAAG 3 cut(s) 163, 2741, 4401
AcvI CACGTG 1 cut(s) 4511
AcyI GRCGYC 2 cut(s) 6, 87
AdeI CACNNNGTG 1 cut(s) 2269
AflII CTTAAG 1 cut(s) 4856
AflIII ACRYGT 2 cut(s) 1739, 2860
AgeI ACCGGT 1 cut(s) 1916
AhlI ACTAGT 1 cut(s) 4811
AjiI CACGTC 1 cut(s) 2778
Alw21I GWGCWC 3 cut(s) 204, 1638, 2444
Alw26I GTCTC 6 cut(s) 140, 329, 2073, 3245, 3588, 3843
AlwI GGATC 9 cut(s) 656, 1651, 1984, 2789, 3100, 4183, 4196, 4475, 4622
AlwNI CAGNNNCTG 4 cut(s) 1523, 2291, 3395, 3806
Ama87I CYCGRG 1 cut(s) 367
Aor13HI TCCGGA 3 cut(s) 1285, 1724, 3592
ApaI GGGCCC 1 cut(s) 1885
ApoI RAATTY 8 cut(s) 725, 2027, 2224, 2714, 3535, 3617, 3964, 4703
ArsI GACNNNNNNTTYG 2 cut(s) 3821, 3853
AsiGI ACCGGT 1 cut(s) 1916
Asp700I GAANNNNTTC 1 cut(s) 4968
Asp718I GGTACC 2 cut(s) 4613, 5082
AspLEI GCGC 1 cut(s) 3100
AsuC2I CCSGG 3 cut(s) 92, 1885, 2267
AsuHPI GGTGA 8 cut(s) 10, 253, 317, 383, 989, 1688, 2281, 3164
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 8 cut(s) 239, 2497, 2671, 3432, 4118, 4343, 4398, 5173
AxyI CCTNAGG 1 cut(s) 1623
BaeGI GKGCMC 2 cut(s) 1885, 3747
BaeI ACNNNNGTAYC 2 cut(s) 4809, 4842
BalI TGGCCA 2 cut(s) 4572, 5032
BamHI GGATCC 1 cut(s) 4188
BanI GGYRCC 5 cut(s) 1180, 1919, 2520, 4613, 5082
BanII GRGCYC 3 cut(s) 204, 1638, 1885
BarI GAAGNNNNNNTAC 4 cut(s) 92, 124, 1387, 1419
BauI CACGAG 2 cut(s) 197, 243
BbrPI CACGTG 1 cut(s) 4511
BbsI GAAGAC 6 cut(s) 516, 938, 1475, 3200, 4331, 4940
Bbv12I GWGCWC 3 cut(s) 204, 1638, 2444
BbvCI CCTCAGC 1 cut(s) 1443
BceAI ACGGC 4 cut(s) 85, 1094, 2447, 2541
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BclI TGATCA 3 cut(s) 607, 2491, 4600
BcnI CCSGG 3 cut(s) 92, 1885, 2267
BcoDI GTCTC 6 cut(s) 140, 329, 2073, 3245, 3588, 3843
BcuI ACTAGT 1 cut(s) 4811
BfrI CTTAAG 1 cut(s) 4856
BfuAI ACCTGC 5 cut(s) 2621, 3010, 3140, 4790, 4948
BglII AGATCT 3 cut(s) 3305, 4369, 4682
Bme18I GGWCC 8 cut(s) 239, 2497, 2671, 3432, 4118, 4343, 4398, 5173
BmeT110I CYCGRG 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 2778
BmrI ACTGGG 2 cut(s) 1888, 2475
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 2 cut(s) 1888, 2475
BoxI GACNNNNGTC 2 cut(s) 3257, 4212
BpiI GAAGAC 6 cut(s) 516, 938, 1475, 3200, 4331, 4940
BpmI CTGGAG 5 cut(s) 258, 425, 1108, 3417, 3717
Bpu10I CCTNAGC 1 cut(s) 1443
BpuEI CTTGAG 3 cut(s) 2365, 4344, 5138
BpuMI CCSGG 3 cut(s) 92, 1885, 2267
BsaAI YACGTR 5 cut(s) 471, 2861, 4165, 4334, 4511
BsaBI GATNNNNATC 1 cut(s) 3336
BsaHI GRCGYC 2 cut(s) 6, 87
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 4 cut(s) 1285, 1724, 1916, 3592
Bse118I RCCGGY 2 cut(s) 1260, 1916
Bse21I CCTNAGG 1 cut(s) 1623
Bse3DI GCAATG 4 cut(s) 1223, 2019, 3525, 4521
Bse8I GATNNNNATC 1 cut(s) 3336
BseAI TCCGGA 3 cut(s) 1285, 1724, 3592
BseGI GGATG 9 cut(s) 3010, 3559, 3634, 3661, 3745, 4559, 5002, 5159, 5243
BseJI GATNNNNATC 1 cut(s) 3336
BseMI GCAATG 4 cut(s) 1223, 2019, 3525, 4521
BseMII CTCAG 9 cut(s) 181, 1434, 1465, 1637, 1833, 3599, 4248, 4997, 5214
BseRI GAGGAG 5 cut(s) 1758, 2100, 2742, 2816, 4612
BseSI GKGCMC 2 cut(s) 1885, 3747
BseYI CCCAGC 2 cut(s) 2511, 2646
BsgI GTGCAG 3 cut(s) 1969, 2226, 3371
Bsh1236I CGCG 3 cut(s) 141, 1610, 3522
Bsh1285I CGRYCG 1 cut(s) 187
BshNI GGYRCC 5 cut(s) 1180, 1919, 2520, 4613, 5082
BshTI ACCGGT 1 cut(s) 1916
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 3 cut(s) 204, 1638, 2444
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 8 cut(s) 92, 1261, 1286, 1725, 1885, 1917, 2267, 3593
BsmAI GTCTC 6 cut(s) 140, 329, 2073, 3245, 3588, 3843
BsmI GAATGC 2 cut(s) 2564, 4050
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 367
Bsp120I GGGCCC 1 cut(s) 1881
Bsp1286I GDGCHC 5 cut(s) 204, 1638, 1885, 2444, 3747
Bsp13I TCCGGA 3 cut(s) 1285, 1724, 3592
Bsp68I TCGCGA 2 cut(s) 141, 1610
BspCNI CTCAG 9 cut(s) 180, 1435, 1464, 1636, 1832, 3598, 4249, 4996, 5215
BspEI TCCGGA 3 cut(s) 1285, 1724, 3592
BspFNI CGCG 3 cut(s) 141, 1610, 3522
BspMAI CTGCAG 6 cut(s) 2131, 2287, 3054, 3151, 3193, 3373
BspMI ACCTGC 5 cut(s) 2621, 3010, 3140, 4790, 4948
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 9 cut(s) 656, 1651, 1984, 2789, 3100, 4183, 4196, 4475, 4622
BspQI GCTCTTC 3 cut(s) 209, 4670, 5243
BspT107I GGYRCC 5 cut(s) 1180, 1919, 2520, 4613, 5082
BspTI CTTAAG 1 cut(s) 4856
BspTNI GGTCTC 1 cut(s) 140
BsrBI CCGCTC 1 cut(s) 452
BsrDI GCAATG 4 cut(s) 1223, 2019, 3525, 4521
BsrFI RCCGGY 2 cut(s) 1260, 1916
BssAI RCCGGY 2 cut(s) 1260, 1916
BssNI GRCGYC 2 cut(s) 6, 87
BssSI CACGAG 2 cut(s) 197, 243
BssT1I CCWWGG 3 cut(s) 3622, 4033, 4391
Bst2BI CACGAG 2 cut(s) 197, 243
Bst6I CTCTTC 7 cut(s) 209, 550, 2076, 2760, 4670, 4912, 5243
BstACI GRCGYC 2 cut(s) 6, 87
BstAFI CTTAAG 1 cut(s) 4856
BstBAI YACGTR 5 cut(s) 471, 2861, 4165, 4334, 4511
BstDSI CCRYGG 3 cut(s) 1971, 2454, 2557
BstEII GGTNACC 1 cut(s) 2852
BstENI CCTNNNNNAGG 1 cut(s) 2504
BstF5I GGATG 9 cut(s) 3010, 3559, 3634, 3661, 3745, 4559, 5002, 5159, 5243
BstFNI CGCG 3 cut(s) 141, 1610, 3522
BstHHI GCGC 1 cut(s) 3100
BstMAI GTCTC 6 cut(s) 140, 329, 2073, 3245, 3588, 3843
BstMCI CGRYCG 1 cut(s) 187
BstNSI RCATGY 7 cut(s) 1171, 1743, 2068, 2190, 2254, 3983, 4947
BstPAI GACNNNNGTC 2 cut(s) 3257, 4212
BstPI GGTNACC 1 cut(s) 2852
BstSLI GKGCMC 2 cut(s) 1885, 3747
BstSNI TACGTA 3 cut(s) 471, 4165, 4334
BstUI CGCG 3 cut(s) 141, 1610, 3522
BstV2I GAAGAC 6 cut(s) 516, 938, 1475, 3200, 4331, 4940
BstX2I RGATCY 7 cut(s) 661, 1643, 1976, 3305, 4188, 4369, 4682
BstXI CCANNNNNNTGG 1 cut(s) 3281
BstYI RGATCY 7 cut(s) 661, 1643, 1976, 3305, 4188, 4369, 4682
Bsu36I CCTNAGG 1 cut(s) 1623
BtgI CCRYGG 3 cut(s) 1971, 2454, 2557
BtrI CACGTC 1 cut(s) 2778
BtsCI GGATG 9 cut(s) 3010, 3559, 3634, 3661, 3745, 4559, 5002, 5159, 5243
BtsI GCAGTG 2 cut(s) 2051, 3378
BtsIMutI CAGTG 7 cut(s) 2051, 2115, 2488, 3261, 3378, 4209, 4227
BtuMI TCGCGA 2 cut(s) 141, 1610
BveI ACCTGC 5 cut(s) 2621, 3010, 3140, 4790, 4948
CaiI CAGNNNCTG 4 cut(s) 1523, 2291, 3395, 3806
CfoI GCGC 1 cut(s) 3100
Cfr10I RCCGGY 2 cut(s) 1260, 1916
CseI GACGC 1 cut(s) 3511
CspAI ACCGGT 1 cut(s) 1916
CspCI CAANNNNNGTGG 2 cut(s) 2436, 2471
DraI TTTAAA 2 cut(s) 3786, 4545
DraIII CACNNNGTG 1 cut(s) 2269
DrdI GACNNNNNNGTC 1 cut(s) 4789
DseDI GACNNNNNNGTC 1 cut(s) 4789
EaeI YGGCCR 3 cut(s) 2554, 4570, 5030
Eam1104I CTCTTC 7 cut(s) 209, 550, 2076, 2760, 4670, 4912, 5243
EarI CTCTTC 7 cut(s) 209, 550, 2076, 2760, 4670, 4912, 5243
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 202, 1636
Eco105I TACGTA 3 cut(s) 471, 4165, 4334
Eco130I CCWWGG 3 cut(s) 3622, 4033, 4391
Eco147I AGGCCT 3 cut(s) 2603, 3502, 4747
Eco24I GRGCYC 3 cut(s) 204, 1638, 1885
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 8 cut(s) 239, 2497, 2671, 3432, 4118, 4343, 4398, 5173
Eco53kI GAGCTC 2 cut(s) 202, 1636
Eco57I CTGAAG 3 cut(s) 163, 2741, 4401
Eco72I CACGTG 1 cut(s) 4511
Eco81I CCTNAGG 1 cut(s) 1623
Eco88I CYCGRG 1 cut(s) 367
Eco91I GGTNACC 1 cut(s) 2852
EcoICRI GAGCTC 2 cut(s) 202, 1636
EcoNI CCTNNNNNAGG 1 cut(s) 2504
EcoO109I RGGNCCY 1 cut(s) 2497
EcoO65I GGTNACC 1 cut(s) 2852
EcoRI GAATTC 1 cut(s) 2714
EcoT14I CCWWGG 3 cut(s) 3622, 4033, 4391
EcoT22I ATGCAT 1 cut(s) 4521
EcoT38I GRGCYC 3 cut(s) 204, 1638, 1885
ErhI CCWWGG 3 cut(s) 3622, 4033, 4391
FalI AAGNNNNNCTT 4 cut(s) 552, 584, 1579, 1611
FauI CCCGC 2 cut(s) 85, 1136
FauNDI CATATG 2 cut(s) 2137, 3679
FbaI TGATCA 3 cut(s) 607, 2491, 4600
FblI GTMKAC 2 cut(s) 3261, 4216
FokI GGATG 9 cut(s) 2997, 3546, 3641, 3668, 3752, 4546, 5009, 5146, 5230
FriOI GRGCYC 3 cut(s) 204, 1638, 1885
GlaI GCGC 1 cut(s) 3099
GsaI CCCAGC 2 cut(s) 2515, 2650
GsuI CTGGAG 5 cut(s) 258, 425, 1108, 3417, 3717
HapII CCGG 8 cut(s) 92, 1261, 1286, 1725, 1885, 1917, 2267, 3593
HgaI GACGC 1 cut(s) 3511
HhaI GCGC 1 cut(s) 3100
Hin1I GRCGYC 2 cut(s) 6, 87
Hin6I GCGC 1 cut(s) 3098
HinP1I GCGC 1 cut(s) 3098
HincII GTYRAC 6 cut(s) 234, 1693, 1914, 3262, 3985, 4207
HindII GTYRAC 6 cut(s) 234, 1693, 1914, 3262, 3985, 4207
HindIII AAGCTT 2 cut(s) 3820, 4859
HpaI GTTAAC 2 cut(s) 1914, 3985
HpaII CCGG 8 cut(s) 92, 1261, 1286, 1725, 1885, 1917, 2267, 3593
HphI GGTGA 8 cut(s) 10, 253, 317, 383, 989, 1688, 2281, 3164
Hpy99I CGWCG 4 cut(s) 71, 146, 155, 164
Hsp92I GRCGYC 2 cut(s) 6, 87
HspAI GCGC 1 cut(s) 3098
Kpn2I TCCGGA 3 cut(s) 1285, 1724, 3592
KpnI GGTACC 2 cut(s) 4617, 5086
Ksp22I TGATCA 3 cut(s) 607, 2491, 4600
KspAI GTTAAC 2 cut(s) 1914, 3985
LguI GCTCTTC 3 cut(s) 209, 4670, 5243
MbiI CCGCTC 1 cut(s) 452
MfeI CAATTG 6 cut(s) 455, 603, 680, 3405, 3447, 4017
MflI RGATCY 7 cut(s) 661, 1643, 1976, 3305, 4188, 4369, 4682
MhlI GDGCHC 5 cut(s) 204, 1638, 1885, 2444, 3747
MlsI TGGCCA 2 cut(s) 4572, 5032
MluNI TGGCCA 2 cut(s) 4572, 5032
MlyI GAGTC 5 cut(s) 255, 1443, 2042, 3853, 4829
MmeI TCCRAC 4 cut(s) 544, 2527, 4438, 4956
Mox20I TGGCCA 2 cut(s) 4572, 5032
Mph1103I ATGCAT 1 cut(s) 4521
MroI TCCGGA 3 cut(s) 1285, 1724, 3592
MroXI GAANNNNTTC 1 cut(s) 4968
MscI TGGCCA 2 cut(s) 4572, 5032
MslI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2185, 2234, 4784, 5211
Msp20I TGGCCA 2 cut(s) 4572, 5032
MspA1I CMGCKG 4 cut(s) 189, 938, 2132, 5129
MspCI CTTAAG 1 cut(s) 4856
MspI CCGG 8 cut(s) 92, 1261, 1286, 1725, 1885, 1917, 2267, 3593
MunI CAATTG 6 cut(s) 455, 603, 680, 3405, 3447, 4017
Mva1269I GAATGC 2 cut(s) 2564, 4050
MvnI CGCG 3 cut(s) 141, 1610, 3522
NciI CCSGG 3 cut(s) 92, 1885, 2267
NdeI CATATG 2 cut(s) 2137, 3679
NheI GCTAGC 1 cut(s) 941
NmuCI GTSAC 6 cut(s) 1706, 2269, 3385, 4506, 4661, 4832
NruI TCGCGA 2 cut(s) 141, 1610
NsiI ATGCAT 1 cut(s) 4521
NspI RCATGY 7 cut(s) 1171, 1743, 2068, 2190, 2254, 3983, 4947
PaqCI CACCTGC 2 cut(s) 3140, 4948
PasI CCCWGGG 3 cut(s) 1303, 3202, 5050
PceI AGGCCT 3 cut(s) 2603, 3502, 4747
PciI ACATGT 1 cut(s) 1739
PciSI GCTCTTC 3 cut(s) 209, 4670, 5243
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 159, 2410
PctI GAATGC 2 cut(s) 2564, 4050
PdmI GAANNNNTTC 1 cut(s) 4968
PflFI GACNNNGTC 3 cut(s) 128, 570, 5138
PflMI CCANNNNNTGG 1 cut(s) 2121
PfoI TCCNGGA 3 cut(s) 90, 4670, 4698
PinAI ACCGGT 1 cut(s) 1916
PleI GAGTC 5 cut(s) 254, 1443, 2042, 3853, 4829
PmaCI CACGTG 1 cut(s) 4511
PmlI CACGTG 1 cut(s) 4511
PpsI GAGTC 5 cut(s) 254, 1443, 2042, 3853, 4829
Ppu21I YACGTR 5 cut(s) 471, 2861, 4165, 4334, 4511
PpuMI RGGWCCY 1 cut(s) 2497
PscI ACATGT 1 cut(s) 1739
PshAI GACNNNNGTC 2 cut(s) 3257, 4212
Psp124BI GAGCTC 2 cut(s) 204, 1638
Psp1406I AACGTT 1 cut(s) 5062
Psp5II RGGWCCY 1 cut(s) 2497
PspCI CACGTG 1 cut(s) 4511
PspEI GGTNACC 1 cut(s) 2852
PspFI CCCAGC 2 cut(s) 2511, 2646
PspOMI GGGCCC 1 cut(s) 1881
PspPPI RGGWCCY 1 cut(s) 2497
PsrI GAACNNNNNNTAC 2 cut(s) 852, 884
PstI CTGCAG 6 cut(s) 2131, 2287, 3054, 3151, 3193, 3373
PstNI CAGNNNCTG 4 cut(s) 1523, 2291, 3395, 3806
PsuI RGATCY 7 cut(s) 661, 1643, 1976, 3305, 4188, 4369, 4682
PsyI GACNNNGTC 3 cut(s) 128, 570, 5138
PvuII CAGCTG 3 cut(s) 938, 2132, 5129
RruI TCGCGA 2 cut(s) 141, 1610
RseI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2185, 2234, 4784, 5211
SacI GAGCTC 2 cut(s) 204, 1638
SalI GTCGAC 1 cut(s) 3260
SapI GCTCTTC 3 cut(s) 209, 4670, 5243
SchI GAGTC 5 cut(s) 255, 1443, 2042, 3853, 4829
SduI GDGCHC 5 cut(s) 204, 1638, 1885, 2444, 3747
SinI GGWCC 8 cut(s) 239, 2497, 2671, 3432, 4118, 4343, 4398, 5173
SmiMI CAYNNNNRTG 8 cut(s) 412, 1436, 1568, 1574, 2185, 2234, 4784, 5211
SmlI CTYRAG 4 cut(s) 2380, 4359, 4856, 5153
SmoI CTYRAG 4 cut(s) 2380, 4359, 4856, 5153
SnaBI TACGTA 3 cut(s) 471, 4165, 4334
SpeI ACTAGT 1 cut(s) 4811
SseBI AGGCCT 3 cut(s) 2603, 3502, 4747
SspI AATATT 3 cut(s) 1966, 2872, 4899
SstI GAGCTC 2 cut(s) 204, 1638
StuI AGGCCT 3 cut(s) 2603, 3502, 4747
StyI CCWWGG 3 cut(s) 3622, 4033, 4391
TaqII GACCGA 2 cut(s) 164, 3449
TatI WGTACW 1 cut(s) 3160
TauI GCSGC 2 cut(s) 452, 4082
TscAI CASTG 7 cut(s) 2058, 2122, 2488, 3261, 3378, 4216, 4227
TseFI GTSAC 6 cut(s) 1706, 2269, 3385, 4506, 4661, 4832
Tsp45I GTSAC 6 cut(s) 1706, 2269, 3385, 4506, 4661, 4832
TspRI CASTG 7 cut(s) 2058, 2122, 2488, 3261, 3378, 4216, 4227
Tth111I GACNNNGTC 3 cut(s) 128, 570, 5138
Van91I CCANNNNNTGG 1 cut(s) 2121
Vha464I CTTAAG 1 cut(s) 4856
VpaK11BI GGWCC 8 cut(s) 239, 2497, 2671, 3432, 4118, 4343, 4398, 5173
XagI CCTNNNNNAGG 1 cut(s) 2504
XapI RAATTY 8 cut(s) 725, 2027, 2224, 2714, 3535, 3617, 3964, 4703
XbaI TCTAGA 1 cut(s) 4972
XceI RCATGY 7 cut(s) 1171, 1743, 2068, 2190, 2254, 3983, 4947
XcmI CCANNNNNNNNNTGG 2 cut(s) 3281, 4580
XmiI GTMKAC 2 cut(s) 3261, 4216
XmnI GAANNNNTTC 1 cut(s) 4968
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 4521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.