Rmu_sc0006234.1_g000016

pfkB family carbohydrate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006234.1
OG0012543
Physical Location & Seq
Forward (+)
51647 .. 52976
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
atgctgatcaacatactgaagaagaatggagtgaacactgagggagtgtgcgtggatgcgcatgctcgaaccgcactggcgtttgtgaccttgagaaaggacggtgagagggagttcatgttctacaggaaccctagtgctgacatgttgctcaaggattcagagctgaacatgggtctgattaagcaggccaagatattccattatggatccataagcctaatatctgagccatgcaaatcggctcatatggcggccatgaaagccgccaaggatgccggtatactgctttcttatgatcccaacgtcaggttgcccctctggccttctgctgatgctgctagagatggcatcaagagcatctggaatcaggctgatttcattaaggtgagcgatgatgaggtgcaattcctaactcaaggagatcctgacaaagaagaagtggttctctccctctggcacgataatctcaaactgcttgttgtcactgatggtgagaagggttgcagatattttactaagaaattcaaaggaaatgtgactggtttctcggttaaagctgttgacacaactggtgcgggtgatgcttttgttggttcatttcttctttccatggctaaagatatgtccatctttgaggatgaagcaaaattgaaggaggctctgtcttttgcaaatgcttgtggagctatctgtactactcagaagggtgcaattcctgctcttccagccgtatccgatgctctcgaactcattaagtcaaaagccaaatag

Protein Analysis

257

Amino Acids

27.99

Weight (kDa)

6.44

Isoelectric Point (pI)

19.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PfkB PF00294 2 - 243 4.9e-53 pfkB family carbohydrate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012543)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10260
fragaria_vesca FvH4_2g00080
malus_domestica MD17G1267100.v1.1
prunus_persica Prupe.8G000700_v2.0.a1
pyrus_communis pycom17g26620
rosa_chinensis RchiOBHm_Chr6g0249341
rosa_laevigata RLG00000015590
rosa_multiflora Rmu_co8334337.1_g000001 Rmu_sc0000278.1_g000022 Rmu_sc0006234.1_g000016
rosa_roxburghii Rroxscaffold_7G00217830
rosa_rugosa Rorug05G0490600.1 Rorug05G0490700.1 Rorug05G0490800.1
rosa_samantha Rh6AG001300 Rh6BG009000 Rh6CG000400 Rh6DG000300
rosa_wichuraiana Rw6G000190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 60
AccI GTMKAC 1 cut(s) 283
AciI CCGC 4 cut(s) 72, 254, 267, 578
AclWI GGATC 4 cut(s) 204, 217, 293, 419
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 1 cut(s) 697
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 2 cut(s) 529, 655
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 3 cut(s) 166, 560, 689
AluI AGCT 3 cut(s) 166, 560, 689
AlwI GGATC 4 cut(s) 204, 217, 293, 419
AoxI GGCC 3 cut(s) 189, 255, 323
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 524
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 61
AsuHPI GGTGA 4 cut(s) 116, 400, 506, 593
BamHI GGATCC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 325
BccI CCATC 3 cut(s) 341, 485, 638
BceAI ACGGC 1 cut(s) 716
BciVI GTATCC 1 cut(s) 745
BclI TGATCA 1 cut(s) 6
BfaI CTAG 2 cut(s) 135, 342
BfmI CTRYAG 1 cut(s) 124
BfuI GTATCC 1 cut(s) 745
BglI GCCNNNNNGGC 1 cut(s) 322
BisI GCNGC 3 cut(s) 255, 267, 339
BlsI GCNGC 3 cut(s) 256, 268, 340
BmiI GGNNCC 2 cut(s) 131, 211
BmsI GCATC 7 cut(s) 46, 265, 325, 360, 369, 574, 730
BpuEI CTTGAG 3 cut(s) 112, 137, 402
BsaJI CCNNGG 2 cut(s) 270, 612
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 104, 134, 650, 680
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 3 cut(s) 81, 547, 577
BseDI CCNNGG 2 cut(s) 270, 612
BseGI GGATG 3 cut(s) 61, 280, 646
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 30, 219, 716
BseNI ACTGG 3 cut(s) 81, 547, 577
BseXI GCAGC 1 cut(s) 325
BshFI GGCC 3 cut(s) 191, 257, 325
BsiSI CCGG 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 309
BsnI GGCC 3 cut(s) 191, 257, 325
Bsp143I GATC 4 cut(s) 6, 209, 298, 424
Bsp19I CCATGG 1 cut(s) 612
BspACI CCGC 4 cut(s) 72, 254, 267, 578
BspANI GGCC 3 cut(s) 191, 257, 325
BspCNI CTCAG 3 cut(s) 31, 220, 715
BspLI GGNNCC 2 cut(s) 131, 211
BspPI GGATC 4 cut(s) 204, 217, 293, 419
BspQI GCTCTTC 1 cut(s) 729
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 3 cut(s) 81, 547, 577
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 270, 612
BssMI GATC 4 cut(s) 6, 209, 298, 424
BssNAI GTATAC 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 270, 612
Bst1107I GTATAC 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 104
Bst6I CTCTTC 1 cut(s) 729
BstAPI GCANNNNNTGC 1 cut(s) 719
BstC8I GCNNGC 2 cut(s) 63, 189
BstDEI CTNAG 4 cut(s) 39, 228, 519, 702
BstDSI CCRYGG 1 cut(s) 612
BstF5I GGATG 3 cut(s) 61, 280, 646
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 4 cut(s) 9, 212, 301, 427
BstMBI GATC 4 cut(s) 6, 209, 298, 424
BstNSI RCATGY 2 cut(s) 65, 148
BstSFI CTRYAG 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 325
BstX2I RGATCY 2 cut(s) 209, 424
BstYI RGATCY 2 cut(s) 209, 424
BstZ17I GTATAC 1 cut(s) 284
BsuI GTATCC 1 cut(s) 745
BsuRI GGCC 3 cut(s) 191, 257, 325
BtgI CCRYGG 1 cut(s) 612
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 61, 280, 646
BtsIMutI CAGTG 3 cut(s) 36, 74, 486
Cac8I GCNNGC 2 cut(s) 63, 189
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 278
Csp6I GTAC 1 cut(s) 696
CviAII CATG 7 cut(s) 62, 118, 145, 172, 234, 259, 613
CviQI GTAC 1 cut(s) 696
DdeI CTNAG 4 cut(s) 39, 228, 519, 702
DpnI GATC 4 cut(s) 8, 211, 300, 426
DpnII GATC 4 cut(s) 6, 209, 298, 424
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 729
EarI CTCTTC 1 cut(s) 729
Eco130I CCWWGG 2 cut(s) 270, 612
Eco57I CTGAAG 1 cut(s) 38
EcoT14I CCWWGG 2 cut(s) 270, 612
ErhI CCWWGG 2 cut(s) 270, 612
FaeI CATG 7 cut(s) 65, 121, 148, 175, 237, 262, 616
FatI CATG 7 cut(s) 61, 117, 144, 171, 233, 258, 612
FauI CCCGC 1 cut(s) 571
FauNDI CATATG 1 cut(s) 249
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 283
Fnu4HI GCNGC 3 cut(s) 255, 267, 339
FokI GGATG 3 cut(s) 68, 287, 653
Fsp4HI GCNGC 3 cut(s) 255, 267, 339
FspAI RTGCGCAY 1 cut(s) 60
FspBI CTAG 2 cut(s) 135, 342
FspI TGCGCA 1 cut(s) 60
GlaI GCGC 1 cut(s) 60
GluI GCNGC 3 cut(s) 255, 267, 339
HaeIII GGCC 3 cut(s) 191, 257, 325
HapII CCGG 1 cut(s) 279
HhaI GCGC 1 cut(s) 61
Hin1II CATG 7 cut(s) 65, 121, 148, 175, 237, 262, 616
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 2 cut(s) 158, 367
HpaII CCGG 1 cut(s) 279
HphI GGTGA 4 cut(s) 116, 400, 506, 593
Hpy166II GTNNAC 3 cut(s) 34, 284, 565
Hpy188I TCNGA 5 cut(s) 163, 180, 229, 705, 739
Hpy188III TCNNGA 4 cut(s) 355, 364, 428, 746
Hpy8I GTNNAC 3 cut(s) 34, 284, 565
HpyAV CCTTC 4 cut(s) 336, 493, 649, 700
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 237, 406, 507, 674, 713
HpyF3I CTNAG 4 cut(s) 39, 228, 519, 702
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 7 cut(s) 65, 121, 148, 175, 237, 262, 616
HspAI GCGC 1 cut(s) 59
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 4 cut(s) 6, 209, 298, 424
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 1 cut(s) 686
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 7 cut(s) 46, 265, 325, 360, 369, 574, 730
MaeI CTAG 2 cut(s) 135, 342
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 3 cut(s) 85, 484, 538
MalI GATC 4 cut(s) 8, 211, 300, 426
MboI GATC 4 cut(s) 6, 209, 298, 424
MboII GAAGA 5 cut(s) 31, 34, 449, 596, 716
MflI RGATCY 2 cut(s) 209, 424
MluCI AATT 4 cut(s) 407, 524, 650, 714
MnlI CCTC 7 cut(s) 34, 102, 329, 394, 464, 631, 652
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 4 cut(s) 183, 384, 555, 756
MslI CAYNNNNRTG 1 cut(s) 386
MspI CCGG 1 cut(s) 279
NcoI CCATGG 1 cut(s) 612
NdeI CATATG 1 cut(s) 249
NdeII GATC 4 cut(s) 6, 209, 298, 424
NlaIII CATG 7 cut(s) 65, 121, 148, 175, 237, 262, 616
NlaIV GGNNCC 2 cut(s) 131, 211
NmuCI GTSAC 3 cut(s) 85, 484, 538
NsbI TGCGCA 1 cut(s) 60
NspI RCATGY 2 cut(s) 65, 148
PaeI GCATGC 1 cut(s) 65
PciI ACATGT 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 729
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 158, 367
PkrI GCNGC 3 cut(s) 256, 268, 340
PscI ACATGT 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 131, 211
PsuI RGATCY 2 cut(s) 209, 424
RsaI GTAC 1 cut(s) 697
RsaNI GTAC 1 cut(s) 696
RseI CAYNNNNRTG 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 4 cut(s) 183, 384, 555, 756
SatI GCNGC 3 cut(s) 255, 267, 339
Sau3AI GATC 4 cut(s) 6, 209, 298, 424
SetI ASST 8 cut(s) 92, 168, 309, 314, 390, 405, 562, 691
SfaNI GCATC 7 cut(s) 46, 265, 325, 360, 369, 574, 730
SfcI CTRYAG 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 3 cut(s) 91, 152, 417
SmoI CTYRAG 3 cut(s) 91, 152, 417
SphI GCATGC 1 cut(s) 65
Sse9I AATT 4 cut(s) 407, 524, 650, 714
SsiI CCGC 4 cut(s) 72, 254, 267, 578
SspMI CTAG 2 cut(s) 135, 342
StyI CCWWGG 2 cut(s) 270, 612
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 67, 747
TasI AATT 4 cut(s) 407, 524, 650, 714
TatI WGTACW 1 cut(s) 695
TauI GCSGC 2 cut(s) 257, 269
TfiI GAWTC 2 cut(s) 158, 367
Tru1I TTAA 4 cut(s) 183, 384, 555, 756
Tru9I TTAA 4 cut(s) 183, 384, 555, 756
TscAI CASTG 3 cut(s) 43, 81, 493
TseFI GTSAC 3 cut(s) 85, 484, 538
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 3 cut(s) 85, 484, 538
TspDTI ATGAA 5 cut(s) 106, 275, 370, 588, 657
TspRI CASTG 3 cut(s) 43, 81, 493
XapI RAATTY 1 cut(s) 524
XceI RCATGY 2 cut(s) 65, 148
XmiI GTMKAC 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 2 cut(s) 135, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.