Rmu_sc0006297.1_g000002

voltage-gated potassium channel subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006297.1
OG0018681
Physical Location & Seq
Forward (+)
860 .. 2245
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
atggcgaaggcgaagtcgaagtcgctgctccagtgctgcctcgaccacggcgtcaacttcttcgacaacgcggaggtctacgcgagcggccgcgccgaggagatcatgggccaggccatacgcgatctgggctggaagcgctccgacatcgtcgtctccaccaagatcagagatttagatcagaagactgagaattggggatcagacttcgttttgcggcttagggaccaaaagattcaaatcatcttgctcggcctattcccaccgccgatagctgatcaaacaccgctgttgcgtctcaatccaccttcatgcaccgcagatgcttctggttcctcccacttcaaatcttacagcaaattgaaattgtgcattgaggcttcgccgatcactccaagttcatccccgccgtcgacgacttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

140

Amino Acids

15.39

Weight (kDa)

8.38

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 2 - 63 1.6e-11 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 50, 152
AccBSI CCGCTC 1 cut(s) 87
AccI GTMKAC 2 cut(s) 78, 413
AccII CGCG 4 cut(s) 71, 83, 93, 123
AciI CCGC 8 cut(s) 71, 87, 91, 217, 266, 287, 318, 407
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 51
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 3 cut(s) 239, 346, 364
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 1 cut(s) 275
AluI AGCT 1 cut(s) 275
Alw26I GTCTC 2 cut(s) 160, 302
AlwI GGATC 1 cut(s) 208
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 4 cut(s) 88, 109, 114, 253
ApeKI GCWGC 2 cut(s) 25, 36
ArsI GACNNNNNNTTYG 2 cut(s) 31, 37
AspLEI GCGC 2 cut(s) 95, 141
AspS9I GGNCC 2 cut(s) 109, 226
AvaII GGWCC 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 191
BbvI GCAGC 2 cut(s) 12, 23
BceAI ACGGC 2 cut(s) 64, 394
BcgI CGANNNNNNTGC 2 cut(s) 7, 41
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 160, 302
BfoI RGCGCY 1 cut(s) 142
BisI GCNGC 5 cut(s) 26, 37, 88, 91, 218
BlsI GCNGC 5 cut(s) 27, 38, 89, 92, 219
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 109, 226
BmiI GGNNCC 2 cut(s) 227, 334
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 191
BpmI CTGGAG 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 221
BsaBI GATNNNNATC 2 cut(s) 177, 239
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 46, 96
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 31
Bse8I GATNNNNATC 2 cut(s) 177, 239
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 2 cut(s) 46, 96
BseGI GGATG 1 cut(s) 401
BseJI GATNNNNATC 2 cut(s) 177, 239
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 31
BseRI GAGGAG 1 cut(s) 113
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 2 cut(s) 12, 23
Bsh1236I CGCG 4 cut(s) 71, 83, 93, 123
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 4 cut(s) 90, 111, 116, 255
BsiEI CGRYCG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 2 cut(s) 160, 302
BsmBI CGTCTC 2 cut(s) 160, 302
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 4 cut(s) 90, 111, 116, 255
Bsp143I GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
BspACI CCGC 8 cut(s) 71, 87, 91, 217, 266, 287, 318, 407
BspANI GGCC 4 cut(s) 90, 111, 116, 255
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 4 cut(s) 71, 83, 93, 123
BspLI GGNNCC 2 cut(s) 227, 334
BspPI GGATC 1 cut(s) 208
BsrBI CCGCTC 1 cut(s) 87
BsrI ACTGG 1 cut(s) 31
BssECI CCNNGG 2 cut(s) 46, 96
BssMI GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 3 cut(s) 189, 221, 420
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 1 cut(s) 401
BstFNI CGCG 4 cut(s) 71, 83, 93, 123
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 2 cut(s) 95, 141
BstKTI GATC 7 cut(s) 105, 127, 168, 181, 203, 280, 390
BstMAI GTCTC 2 cut(s) 160, 302
BstMBI GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 2 cut(s) 129, 138
BstNI CCWGG 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 111
BstUI CGCG 4 cut(s) 71, 83, 93, 123
BstV1I GCAGC 2 cut(s) 12, 23
BstV2I GAAGAC 1 cut(s) 191
BstZI CGGCCG 1 cut(s) 88
BsuRI GGCC 4 cut(s) 90, 111, 116, 255
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 401
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 85
CciNI GCGGCCGC 1 cut(s) 88
CfoI GCGC 2 cut(s) 95, 141
Cfr13I GGNCC 2 cut(s) 109, 226
CseI GACGC 2 cut(s) 40, 284
CviAII CATG 2 cut(s) 106, 312
CviJI RGCY 8 cut(s) 90, 111, 116, 132, 220, 255, 275, 380
CviKI_1 RGCY 8 cut(s) 90, 111, 116, 132, 220, 255, 275, 380
DdeI CTNAG 3 cut(s) 189, 221, 420
DpnI GATC 7 cut(s) 104, 126, 167, 180, 202, 279, 389
DpnII GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
DrdI GACNNNNNNGTC 2 cut(s) 50, 152
DseDI GACNNNNNNGTC 2 cut(s) 50, 152
EaeI YGGCCR 1 cut(s) 88
EagI CGGCCG 1 cut(s) 88
EclXI CGGCCG 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 226
Eco47III AGCGCT 1 cut(s) 140
Eco52I CGGCCG 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 111
Esp3I CGTCTC 2 cut(s) 160, 302
FaeI CATG 2 cut(s) 109, 315
FaiI YATR 3 cut(s) 107, 119, 313
FaqI GGGAC 1 cut(s) 239
FatI CATG 2 cut(s) 105, 311
FauI CCCGC 1 cut(s) 414
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 2 cut(s) 78, 413
Fnu4HI GCNGC 5 cut(s) 26, 37, 88, 91, 218
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 5 cut(s) 26, 37, 88, 91, 218
GlaI GCGC 2 cut(s) 94, 140
GluI GCNGC 5 cut(s) 26, 37, 88, 91, 218
GsuI CTGGAG 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 90, 111, 116, 255
HgaI GACGC 2 cut(s) 40, 284
HhaI GCGC 2 cut(s) 95, 141
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 2 cut(s) 109, 315
Hin6I GCGC 2 cut(s) 93, 139
HinP1I GCGC 2 cut(s) 93, 139
HincII GTYRAC 2 cut(s) 55, 414
HindII GTYRAC 2 cut(s) 55, 414
HinfI GANTC 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 55, 79, 414
Hpy188I TCNGA 4 cut(s) 145, 170, 183, 205
Hpy8I GTNNAC 3 cut(s) 55, 79, 414
Hpy99I CGWCG 3 cut(s) 155, 415, 418
HpyAV CCTTC 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 315, 372
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 138
HpyF3I CTNAG 3 cut(s) 189, 221, 420
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 2 cut(s) 109, 315
HspAI GCGC 2 cut(s) 93, 139
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
LmnI GCTCC 2 cut(s) 33, 146
LpnPI CCDG 6 cut(s) 44, 98, 113, 118, 125, 315
Lsp1109I GCAGC 2 cut(s) 12, 23
LweI GCATC 1 cut(s) 313
MalI GATC 7 cut(s) 104, 126, 167, 180, 202, 279, 389
MbiI CCGCTC 1 cut(s) 87
MboI GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
MboII GAAGA 2 cut(s) 52, 196
MluCI AATT 3 cut(s) 193, 359, 365
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 5 cut(s) 50, 67, 91, 346, 370
MslI CAYNNNNRTG 1 cut(s) 310
MspA1I CMGCKG 1 cut(s) 289
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 4 cut(s) 71, 83, 93, 123
MwoI GCNNNNNNNGC 2 cut(s) 129, 138
NdeII GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
NlaIII CATG 2 cut(s) 109, 315
NlaIV GGNNCC 2 cut(s) 227, 334
NmeAIII GCCGAG 2 cut(s) 121, 231
NotI GCGGCCGC 1 cut(s) 88
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PfeI GAWTC 1 cut(s) 235
PflFI GACNNNGTC 1 cut(s) 149
PkrI GCNGC 5 cut(s) 27, 38, 89, 92, 219
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 227, 334
PspPI GGNCC 2 cut(s) 109, 226
PsyI GACNNNGTC 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 310
SalI GTCGAC 1 cut(s) 412
SatI GCNGC 5 cut(s) 26, 37, 88, 91, 218
Sau3AI GATC 7 cut(s) 102, 124, 165, 178, 200, 277, 387
Sau96I GGNCC 2 cut(s) 109, 226
ScrFI CCNGG 1 cut(s) 113
SetI ASST 3 cut(s) 78, 277, 310
SfaNI GCATC 1 cut(s) 313
SgrDI CGTCGACG 1 cut(s) 412
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 310
Sse9I AATT 3 cut(s) 193, 359, 365
SsiI CCGC 8 cut(s) 71, 87, 91, 217, 266, 287, 318, 407
StyD4I CCNGG 1 cut(s) 111
TaqI TCGA 4 cut(s) 17, 42, 63, 413
TasI AATT 3 cut(s) 193, 359, 365
TauI GCSGC 3 cut(s) 90, 93, 220
TfiI GAWTC 1 cut(s) 235
TscAI CASTG 1 cut(s) 38
TseI GCWGC 2 cut(s) 25, 36
TspDTI ATGAA 2 cut(s) 300, 390
TspRI CASTG 1 cut(s) 38
Tth111I GACNNNGTC 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 226
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmiI GTMKAC 2 cut(s) 78, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.