Rmu_sc0006558.1_g000026

Zinc knuckle family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006558.1
OG0019242
Physical Location & Seq
Reverse (-)
126993 .. 133254
6262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 756 bp
atgaatggatccgcccctgcccatagatcctcttccgactcctccttctccggtagtgattctgatacaatttcccaaatcaatcaaacatttcatgaactccaattacatagaatcgttcatccaaagaaacagcctttccaaaattggacagaacgccctttaccattagatattcaacccactacttatcccagttatcaattttcagtctcttccgataaaacctatgaatggaacattgataatttatccgaacaggaactcttaaataaattacatcacatgtccatggcagccaatagctacgtcaccaatcataatttcagccagcctgaaattattgatattctctctacaggattcacaggaatgcttcattcctggtgggaaaaacatctcactcctgattcaagagcagccattaaacaagcagctaagcatgacgaagatggaattccaatctttgatgaacacattgtcaagagctccagtcactccaatgccaccccaaacgacatccacttcccagtcactccaatgcccacccaaacgacatccacttcccagtcacaacctaagtccctcaagtcacattcctcttccaaatccaaggggaaaacaaagtcaacaaaatcagatacatcctccaagttagaagagttgaaagagttagcaaaaaggctcttagcacaagcagctgatctcagtaatgatgatgatgaatctgaagaagaagaagaaatttcttcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MP RVP zf-CCHC

Protein Analysis

251

Amino Acids

28.12

Weight (kDa)

5.55

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7746 PF24925 72 - 147 6.9e-22 Domain of unknown function (DUF7746)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019242)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g39761
prunus_persica Prupe.4G270900_v2.0.a1
pyrus_communis pycom01g03940 pycom03g01680 pycom13g24850
rosa_multiflora Rmu_sc0000235.1_g000028 Rmu_sc0000365.1_g000044 Rmu_sc0006558.1_g000026

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 12
AclWI GGATC 3 cut(s) 3, 16, 21
AcsI RAATTY 2 cut(s) 456, 744
AcuI CTGAAG 1 cut(s) 750
AfiI CCNNNNNNNGG 1 cut(s) 234
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 3 cut(s) 179, 414, 667
AjnI CCWGG 1 cut(s) 383
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 4 cut(s) 306, 437, 489, 701
AluI AGCT 4 cut(s) 306, 437, 489, 701
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 3 cut(s) 3, 16, 21
ApeKI GCWGC 4 cut(s) 296, 419, 434, 698
ApoI RAATTY 2 cut(s) 456, 744
AsuHPI GGTGA 1 cut(s) 304
BamHI GGATCC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 491
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 4 cut(s) 308, 431, 446, 710
BccI CCATC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 217
BfmI CTRYAG 1 cut(s) 357
BisI GCNGC 4 cut(s) 297, 420, 435, 699
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 4 cut(s) 298, 421, 436, 700
Bme1390I CCNGG 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 10
BmrFI CCNGG 1 cut(s) 385
BmrI ACTGGG 3 cut(s) 189, 524, 562
BmuI ACTGGG 3 cut(s) 189, 524, 562
BpmI CTGGAG 1 cut(s) 475
Bpu1102I GCTNAGC 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 572
BsaJI CCNNGG 2 cut(s) 291, 612
BsaWI WCCGGW 1 cut(s) 50
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse1I ACTGG 4 cut(s) 195, 492, 530, 568
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 291, 612
BseGI GGATG 4 cut(s) 121, 519, 557, 644
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 721
BseNI ACTGG 4 cut(s) 195, 492, 530, 568
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 4 cut(s) 308, 431, 446, 710
BsiHKAI GWGCWC 1 cut(s) 491
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 378
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 3 cut(s) 8, 26, 703
Bsp1720I GCTNAGC 1 cut(s) 438
Bsp19I CCATGG 1 cut(s) 291
BspACI CCGC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 720
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 10
BspPI GGATC 3 cut(s) 3, 16, 21
BsrI ACTGG 4 cut(s) 195, 492, 530, 568
BssECI CCNNGG 2 cut(s) 291, 612
BssMI GATC 3 cut(s) 8, 26, 703
BssT1I CCWWGG 2 cut(s) 291, 612
Bst2UI CCWGG 1 cut(s) 385
Bst6I CTCTTC 4 cut(s) 37, 220, 607, 654
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 4 cut(s) 438, 579, 688, 707
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 4 cut(s) 121, 519, 557, 644
BstKTI GATC 3 cut(s) 11, 29, 706
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 3 cut(s) 8, 26, 703
BstMWI GCNNNNNNNGC 1 cut(s) 698
BstNI CCWGG 1 cut(s) 385
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 357
BstV1I GCAGC 4 cut(s) 308, 431, 446, 710
BstX2I RGATCY 2 cut(s) 8, 26
BstYI RGATCY 2 cut(s) 8, 26
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 4 cut(s) 121, 519, 557, 644
Cac8I GCNNGC 1 cut(s) 332
CciI TCATGA 1 cut(s) 94
CviAII CATG 4 cut(s) 95, 286, 292, 443
DdeI CTNAG 4 cut(s) 438, 579, 688, 707
DpnI GATC 3 cut(s) 10, 28, 705
DpnII GATC 3 cut(s) 8, 26, 703
Eam1104I CTCTTC 4 cut(s) 37, 220, 607, 654
EarI CTCTTC 4 cut(s) 37, 220, 607, 654
Ecl136II GAGCTC 1 cut(s) 489
Eco130I CCWWGG 2 cut(s) 291, 612
Eco24I GRGCYC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 750
EcoICRI GAGCTC 1 cut(s) 489
EcoRI GAATTC 1 cut(s) 456
EcoRII CCWGG 1 cut(s) 383
EcoT14I CCWWGG 2 cut(s) 291, 612
EcoT38I GRGCYC 1 cut(s) 491
ErhI CCWWGG 2 cut(s) 291, 612
FaeI CATG 4 cut(s) 98, 289, 295, 446
FaiI YATR 8 cut(s) 24, 96, 111, 231, 287, 293, 321, 444
FaqI GGGAC 1 cut(s) 568
FatI CATG 4 cut(s) 94, 285, 291, 442
Fnu4HI GCNGC 4 cut(s) 297, 420, 435, 699
FokI GGATG 4 cut(s) 108, 506, 544, 631
FriOI GRGCYC 1 cut(s) 491
Fsp4HI GCNGC 4 cut(s) 297, 420, 435, 699
GluI GCNGC 4 cut(s) 297, 420, 435, 699
GsuI CTGGAG 1 cut(s) 475
HapII CCGG 1 cut(s) 51
Hin1II CATG 4 cut(s) 98, 289, 295, 446
HincII GTYRAC 1 cut(s) 630
HindII GTYRAC 1 cut(s) 630
HinfI GANTC 6 cut(s) 38, 59, 114, 363, 410, 725
HpaII CCGG 1 cut(s) 51
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 1 cut(s) 630
Hpy188I TCNGA 6 cut(s) 37, 64, 220, 256, 640, 730
Hpy188III TCNNGA 5 cut(s) 95, 407, 414, 484, 753
Hpy8I GTNNAC 1 cut(s) 630
HpyAV CCTTC 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 1 cut(s) 698
HpyF3I CTNAG 4 cut(s) 438, 579, 688, 707
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 4 cut(s) 98, 289, 295, 446
Kzo9I GATC 3 cut(s) 8, 26, 703
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 4 cut(s) 308, 431, 446, 710
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 5 cut(s) 310, 494, 532, 570, 591
MalI GATC 3 cut(s) 10, 28, 705
MboI GATC 3 cut(s) 8, 26, 703
MflI RGATCY 2 cut(s) 8, 26
MhlI GDGCHC 1 cut(s) 491
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 5 cut(s) 40, 52, 596, 610, 658
MseI TTAA 2 cut(s) 269, 426
MslI CAYNNNNRTG 4 cut(s) 290, 371, 501, 539
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 385
Mva1269I GAATGC 1 cut(s) 378
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 1 cut(s) 698
NcoI CCATGG 1 cut(s) 291
NdeII GATC 3 cut(s) 8, 26, 703
NlaIII CATG 4 cut(s) 98, 289, 295, 446
NlaIV GGNNCC 1 cut(s) 10
NmuCI GTSAC 5 cut(s) 310, 494, 532, 570, 591
NspI RCATGY 1 cut(s) 289
PagI TCATGA 1 cut(s) 94
PciI ACATGT 1 cut(s) 285
PctI GAATGC 1 cut(s) 378
PfeI GAWTC 5 cut(s) 59, 114, 363, 410, 725
PkrI GCNGC 4 cut(s) 298, 421, 436, 700
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PscI ACATGT 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 1 cut(s) 10
PsrI GAACNNNNNNTAC 4 cut(s) 90, 122, 147, 179
PsuI RGATCY 2 cut(s) 8, 26
PvuII CAGCTG 1 cut(s) 701
RseI CAYNNNNRTG 4 cut(s) 290, 371, 501, 539
SacI GAGCTC 1 cut(s) 491
SaqAI TTAA 2 cut(s) 269, 426
SatI GCNGC 4 cut(s) 297, 420, 435, 699
Sau3AI GATC 3 cut(s) 8, 26, 703
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 385
SduI GDGCHC 1 cut(s) 491
SetI ASST 7 cut(s) 230, 308, 312, 439, 491, 580, 703
SfcI CTRYAG 1 cut(s) 357
SmiMI CAYNNNNRTG 4 cut(s) 290, 371, 501, 539
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
SsiI CCGC 1 cut(s) 12
SstI GAGCTC 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 383
StyI CCWWGG 2 cut(s) 291, 612
TaiI ACGT 1 cut(s) 312
TfiI GAWTC 5 cut(s) 59, 114, 363, 410, 725
Tru1I TTAA 2 cut(s) 269, 426
Tru9I TTAA 2 cut(s) 269, 426
TseFI GTSAC 5 cut(s) 310, 494, 532, 570, 591
TseI GCWGC 4 cut(s) 296, 419, 434, 698
Tsp45I GTSAC 5 cut(s) 310, 494, 532, 570, 591
TspDTI ATGAA 8 cut(s) 17, 83, 110, 111, 246, 368, 486, 738
XapI RAATTY 2 cut(s) 456, 744
XceI RCATGY 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.