Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0006845.1

Orthogroup ID

OG0013378

Physical Location & Seq

Genomic Coordinates Reverse (-)

23289 .. 28038

Gene Structure 4750 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3537 bp

atggacacattctctatcctctcctcctcaccctctcacaccttcttcacgcgcctcctctctctcaaaccctcacctcctcatcccttacttcaccacctcacccaaaccctagccttctcttcccccaaatttccacacactcaaaatctactccgattccgagtcactgtccagtccccaagctccgccttccccgccaaatctcaactctccgacgccgacgacgatgaggaggaagacgagttcgaagaggacgacgacgaagacgtcgcggccgatgagtacgatgacatttccggcgatatgtcggacgggctcgaacaaagcgacgatgaaattgagatggaggactccatggccggtgcagagccgccggcgattcgcgacgaggagttccggtggaagagagtggagaagctctgcgaagatgtgaaacagttcggcgaggaaatgattgacgacggcgctctcgcctcaatctacgatttccgaatcgataaatttcagagattagctatacaagcattcttgagaggttcatcagtagttgtgtctgcgccgacgagtagcggaaagactttgatagcagaagctgctgctgtagcaaccgtagccaagggaagaagattgttctacacaactccgcttaaggccttatctaatcagaagtttcgcgaatttcgtgaaactttcggtgaaaataatgttggtcttctcacgggagattcggcagtaaacaaagatgctcaggttttgattatgaccacagagattttgcgcaacatgttgtatcagagcgttggaatgtcttctgctcgggactcactttttcacgtggatgtaatagtcttggatgaagttcattatttaagtgacatatctcggggtacagtgtgggaagaaattgttatttattccccaaaagtggttcaactcatatgtttgtccgcaacagttgctaatccagatgaattagctggttggattggtcagattcatggtaaaactgaattggtaacttcaacaaagcgtccagtaccactgacttggcatttctcaacaaagacttctttattaccacttcttgataagacgggaaaacacatgaataggaggttgtcagtcaattatctgcaacttagtgctgctgcaacaaaatctaataaagatgatggatctagaaggaggagttcaagaagacgggctagtgagacgagctatgatgacagtactggtaacatgtctagacgaccactttcaaagaatgacatcaacttaatataccgttcacaagtgcctcaagttactgatacattatggcaccttaaggctagggatatgctaccagcagtatggtttatttttagcaggaaaggttgtgatgctgctgttcagtacattcaagattgcaaacttttagatgactgtgaagcaagtgaggttgaattagcgttgaagaggttccgcattaagtatccggatgctatcagggagagtgcagtgaaggggttactgcgaggggttgctgcccatcatgctggctgtctacccttatggaaatcattcatagaagagctgtttcagcgtggtcttgttaaggttgtatttgctacagaaacacttgctgctggaatcaatatgcctgctaggacagccattattgcatccctcagcaaaaggagtgacagtggacgtaccctattgagctcaaatgaactacttcaaatggctggccgtgctggacgtagaggcacagatgaaaggggtcatgtagtgcttattcaaaatccttacgagggtgctgaagctggttgcaaggttctttttgctggacttgaacctctggtatcacagtttactgcttcatatggaatggtgctaaatcttcttgccggttcaaaagtcactcgtagatcaagtgaatcagatgatacaaaagcctcccaatctgggcgaactttggacgaagctagaaagctagtggagcaaagttttggaaactatgttggcagcaatgttatgcttgctgcaaaagaggagcttgctagaattgagaaagaaatagagatgctgactttggaaattagcgatgacgccatcgacagaaaaagcaggaagctcatgtctggaccagcctacaaggagattgcagatctacaggaagaattaagggcagagaaacgcctaaggactgaacttcggaaaagaatggaatctcagaagctttcttcactacgacccttattaaaggagtgtgaaaatgggcacttgccctttttgtgcttgcagtataaagattcagaaggagttcaacactccatcccggctgtttatttggggaaggttgagtttctgagttgctcaaagcttaagaacatggtttctgtggacgattcttttgcactaaatgcagtagcagcacagtttgaaccaacttcagtttttgaaccatcatattatgcggctcttggctctgacaattcttggtacctatttacagaaaagtggatcaaaacaatttataagacaggctttccaaatgttgctttagctcaaggagatgctttgcctcgggaaataatgagcacgcttcttgataagacagaaatgaagtgggagaagcttgcggagtctgatcttggtggtttttggggcatggaaggatctcttgagacatggtcttggagtttgaatgttccagtcttgaatagtctttctgaacatgatgagcttttactcaagtcagaagcatataatcatgctatagggtgttataaagaacaaagaagtaaagtttcacgtctgaagaaaaagatatcgcgtacacaaggcttcagagaatacaagaagatcattgacatggccaactttacagaggagaagatcaagcgcttgaagggtagatcaagacgtttgactaatcgaatagagcagattgaaccatctggttggaaagagttcttgcagatcagcaatgttatacatgaaactcgggcattagatattaacactcataccatatttccacttggtgaaactgcagccgctattcgtggagaaaacgaactgtggcttgccatggttcttcgaaataagatcttactaagtttaaagcctacagaacttgctgctgtgtgcgcgagtttagtatctgaagggatcaaaattcgtccgtggaaaaataacagctacatatacgaaccttcaagtacagtaattgacgtggtgagcttcttggatgagcaaagaagcacttttttacagcttcaggaaaagcatggggtaaataaggcttgctatttggatacccagtttgcaggtatggttgaagcctgggtttctgggttgacttggagggaaatcatgatggattgtgcaatggacgagggagatttagcccgccttctacggcgaactatagatctgctggtgcaaatccccaaattgcctgatattgaccccgtcttgcagagcaacgcaaagacggcgtcaaatatcatggaccgccctccgataagtgaattagctggttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000373 GO:0000375 GO:0000377 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010494 GO:0010496 GO:0010497 GO:0010501 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035770 GO:0036464 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C

Protein Analysis

1178

Amino Acids

132.22

Weight (kDa)

5.79

Isoelectric Point (pI)

52.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	168 - 324	2.5e-27	DEAD/DEAH box helicase
ResIII	PF04851	170 - 320	4.6e-10	Type III restriction enzyme, res subunit
DEXQc_Suv3	PF22527	186 - 305	1.6e-07	DEXQ-box helicase domain of Suv3
SH3_ISE2	PF25446	742 - 920	6.2e-87	ISE2, SH3 domain
DSHCT	PF08148	1007 - 1173	1.9e-34	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70070
fragaria_vesca	FvH4_4g35290 FvH4_4g35290 FvH4_4g35290
malus_domestica	MD16G1083800.v1.1
prunus_persica	Prupe.1G262200_v2.0.a1
pyrus_communis	pycom16g07280
rosa_chinensis	RchiOBHm_Chr4g0444891
rosa_laevigata	RLG00000005791
rosa_multiflora	Rmu_sc0006845.1_g000003 Rmu_sc0013783.1_g000003
rosa_roxburghii	Rroxscaffold_5G00385390
rosa_rugosa	Rorug04G0355800
rosa_samantha	Rh4AG417600 Rh4BG429400 Rh4CG444100 Rh4DG425300
rosa_wichuraiana	Rw4G035790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2526, 2778
AasI	GACNNNNNNGTC	1 cut(s)	269
AatII	GACGTC	1 cut(s)	273
Acc16I	TGCGCA	1 cut(s)	782
Acc36I	ACCTGC	1 cut(s)	3311
Acc65I	GGTACC	1 cut(s)	2490
AccB1I	GGYRCC	2 cut(s)	1323, 2490
AccI	GTMKAC	1 cut(s)	1549
AccII	CGCG	6 cut(s)	52, 275, 387, 678, 2824, 3143
AccIII	TCCGGA	1 cut(s)	1480
AclWI	GGATC	4 cut(s)	1186, 2519, 2674, 3170
AcoI	YGGCCR	4 cut(s)	276, 360, 1735, 2865
AcsI	RAATTY	4 cut(s)	131, 503, 680, 3168
AcuI	CTGAAG	6 cut(s)	1827, 2424, 2821, 2828, 3177, 3254
AcvI	CACGTG	1 cut(s)	838
AcyI	GRCGYC	4 cut(s)	219, 270, 2097, 3491
AdeI	CACNNNGTG	1 cut(s)	3035
AfaI	GTAC	9 cut(s)	287, 892, 1041, 1234, 1400, 1699, 2492, 2827, 3214
AfeI	AGCGCT	1 cut(s)	2894
AfiI	CCNNNNNNNGG	3 cut(s)	928, 1799, 1953
AflII	CTTAAG	3 cut(s)	650, 1328, 2372
AflIII	ACRYGT	2 cut(s)	786, 1242
AjiI	CACGTC	2 cut(s)	2804, 3226
AjnI	CCWGG	1 cut(s)	3335
AjuI	GAANNNNNNNTTGG	2 cut(s)	693, 725
AloI	GAACNNNNNNTCC	2 cut(s)	230, 262
Alw21I	GWGCWC	2 cut(s)	1712, 2591
Alw26I	GTCTC	2 cut(s)	1208, 2669
AlwI	GGATC	4 cut(s)	1186, 2519, 2674, 3170
AlwNI	CAGNNNCTG	1 cut(s)	596
Ama87I	CYCGRG	4 cut(s)	819, 885, 2574, 2994
Aor13HI	TCCGGA	1 cut(s)	1480
Aor51HI	AGCGCT	1 cut(s)	2894
AoxI	GGCC	5 cut(s)	276, 360, 654, 1735, 2865
ApoI	RAATTY	4 cut(s)	131, 503, 680, 3168
ArsI	GACNNNNNNTTYG	4 cut(s)	2384, 2416, 3476, 3508
Asp700I	GAANNNNTTC	7 cut(s)	440, 811, 1463, 1565, 1722, 2310, 2960
Asp718I	GGTACC	1 cut(s)	2490
AspLEI	GCGC	6 cut(s)	54, 470, 562, 783, 2895, 3143
AspS9I	GGNCC	2 cut(s)	2132, 3505
AsuC2I	CCSGG	1 cut(s)	2327
AsuHPI	GGTGA	7 cut(s)	21, 66, 86, 94, 710, 3047, 3241
AsuII	TTCGAA	2 cut(s)	249, 3091
AvaI	CYCGRG	4 cut(s)	819, 885, 2574, 2994
AvaII	GGWCC	2 cut(s)	2132, 3505
AxyI	CCTNAGG	1 cut(s)	2189
BaeGI	GKGCMC	1 cut(s)	2271
BalI	TGGCCA	1 cut(s)	2867
BanI	GGYRCC	2 cut(s)	1323, 2490
BanII	GRGCYC	2 cut(s)	321, 1712
BbrPI	CACGTG	1 cut(s)	838
BbsI	GAAGAC	5 cut(s)	246, 273, 707, 804, 1207
Bbv12I	GWGCWC	2 cut(s)	1712, 2591
BbvCI	CCTCAGC	1 cut(s)	1673
BccI	CCATC	8 cut(s)	340, 1169, 1542, 2108, 2330, 2461, 2953, 3364
BceAI	ACGGC	4 cut(s)	481, 1722, 3428, 3504
BcgI	CGANNNNNNTGC	2 cut(s)	2384, 2418
BciT130I	CCWGG	1 cut(s)	3337
BciVI	GTATCC	2 cut(s)	1488, 3301
BcnI	CCSGG	1 cut(s)	2327
BcoDI	GTCTC	2 cut(s)	1208, 2669
BfaI	CTAG	9 cut(s)	113, 1182, 1209, 1248, 1335, 1650, 1974, 1982, 2049
BfmI	CTRYAG	7 cut(s)	603, 1614, 2159, 2766, 3042, 3120, 3420
BfoI	RGCGCY	2 cut(s)	471, 2896
BfrI	CTTAAG	3 cut(s)	650, 1328, 2372
BfuAI	ACCTGC	1 cut(s)	3311
BfuI	GTATCC	2 cut(s)	1488, 3301
BglII	AGATCT	3 cut(s)	2155, 3099, 3424
BmcAI	AGTACT	1 cut(s)	1234
Bme1390I	CCNGG	2 cut(s)	2327, 3337
Bme18I	GGWCC	2 cut(s)	2132, 3505
BmeT110I	CYCGRG	4 cut(s)	819, 885, 2574, 2994
BmgBI	CACGTC	2 cut(s)	2804, 3226
BmgT120I	GGNCC	2 cut(s)	2132, 3505
BmiI	GGNNCC	3 cut(s)	1325, 1466, 2492
BmrFI	CCNGG	2 cut(s)	2327, 3337
BmrI	ACTGGG	1 cut(s)	3307
BmsI	GCATC	6 cut(s)	736, 1375, 1474, 1676, 2061, 2554
BmuI	ACTGGG	1 cut(s)	3307
BpiI	GAAGAC	5 cut(s)	246, 273, 707, 804, 1207
Bpu10I	CCTNAGC	2 cut(s)	750, 1673
Bpu14I	TTCGAA	2 cut(s)	249, 3091
BpuEI	CTTGAG	5 cut(s)	553, 1287, 2541, 2693, 2726
BpuMI	CCSGG	1 cut(s)	2327
Bsa29I	ATCGAT	1 cut(s)	498
BsaAI	YACGTR	1 cut(s)	838
BsaHI	GRCGYC	4 cut(s)	219, 270, 2097, 3491
BsaJI	CCNNGG	6 cut(s)	357, 618, 2573, 3081, 3176, 3336
BsaWI	WCCGGW	2 cut(s)	399, 1480
BsaXI	ACNNNNNCTCC	4 cut(s)	386, 416, 1675, 1705
Bsc4I	CCNNNNNNNGG	3 cut(s)	928, 1799, 1953
Bse118I	RCCGGY	3 cut(s)	362, 376, 1895
Bse1I	ACTGG	5 cut(s)	175, 1037, 1240, 2702, 3313
Bse21I	CCTNAGG	1 cut(s)	2189
Bse3DI	GCAATG	3 cut(s)	2023, 2983, 3387
BseAI	TCCGGA	1 cut(s)	1480
BseBI	CCWGG	1 cut(s)	3337
BseCI	ATCGAT	1 cut(s)	498
BseDI	CCNNGG	6 cut(s)	357, 618, 2573, 3081, 3176, 3336
BseGI	GGATG	7 cut(s)	82, 847, 862, 1489, 1667, 2322, 3247
BseLI	CCNNNNNNNGG	3 cut(s)	928, 1799, 1953
BseMI	GCAATG	3 cut(s)	2023, 2983, 3387
BseMII	CTCAG	4 cut(s)	764, 1687, 2234, 2348
BseNI	ACTGG	5 cut(s)	175, 1037, 1240, 2702, 3313
BseRI	GAGGAG	9 cut(s)	13, 16, 47, 69, 248, 407, 1204, 2054, 2894
BseSI	GKGCMC	1 cut(s)	2271
BseX3I	CGGCCG	1 cut(s)	276
BsgI	GTGCAG	2 cut(s)	387, 1521
Bsh1236I	CGCG	6 cut(s)	52, 275, 387, 678, 2824, 3143
Bsh1285I	CGRYCG	1 cut(s)	279
BshFI	GGCC	5 cut(s)	278, 362, 656, 1737, 2867
BshNI	GGYRCC	2 cut(s)	1323, 2490
BshVI	ATCGAT	1 cut(s)	498
BsiEI	CGRYCG	1 cut(s)	279
BsiHKAI	GWGCWC	2 cut(s)	1712, 2591
BsiHKCI	CYCGRG	4 cut(s)	819, 885, 2574, 2994
BsiSI	CCGG	7 cut(s)	300, 363, 377, 400, 1481, 1896, 2327
BslFI	GGGAC	2 cut(s)	163, 836
BslI	CCNNNNNNNGG	3 cut(s)	928, 1799, 1953
BsmAI	GTCTC	2 cut(s)	1208, 2669
BsmBI	CGTCTC	1 cut(s)	1208
BsmFI	GGGAC	2 cut(s)	163, 836
BsmI	GAATGC	1 cut(s)	527
BsnI	GGCC	5 cut(s)	278, 362, 656, 1737, 2867
BsoBI	CYCGRG	4 cut(s)	819, 885, 2574, 2994
Bsp119I	TTCGAA	2 cut(s)	249, 3091
Bsp1286I	GDGCHC	4 cut(s)	321, 1712, 2271, 2591
Bsp13I	TCCGGA	1 cut(s)	1480
Bsp19I	CCATGG	2 cut(s)	357, 3081
Bsp68I	TCGCGA	2 cut(s)	387, 678
BspANI	GGCC	5 cut(s)	278, 362, 656, 1737, 2867
BspCNI	CTCAG	4 cut(s)	763, 1686, 2233, 2349
BspDI	ATCGAT	1 cut(s)	498
BspEI	TCCGGA	1 cut(s)	1480
BspFNI	CGCG	6 cut(s)	52, 275, 387, 678, 2824, 3143
BspHI	TCATGA	1 cut(s)	3366
BspLI	GGNNCC	3 cut(s)	1325, 1466, 2492
BspMAI	CTGCAG	1 cut(s)	3046
BspMI	ACCTGC	1 cut(s)	3311
BspPI	GGATC	4 cut(s)	1186, 2519, 2674, 3170
BspQI	GCTCTTC	1 cut(s)	1569
BspT104I	TTCGAA	2 cut(s)	249, 3091
BspT107I	GGYRCC	2 cut(s)	1323, 2490
BspTI	CTTAAG	3 cut(s)	650, 1328, 2372
BsrDI	GCAATG	3 cut(s)	2023, 2983, 3387
BsrFI	RCCGGY	3 cut(s)	362, 376, 1895
BsrI	ACTGG	5 cut(s)	175, 1037, 1240, 2702, 3313
BssAI	RCCGGY	3 cut(s)	362, 376, 1895
BssECI	CCNNGG	6 cut(s)	357, 618, 2573, 3081, 3176, 3336
BssNI	GRCGYC	4 cut(s)	219, 270, 2097, 3491
BssT1I	CCWWGG	3 cut(s)	357, 618, 3081
Bst2UI	CCWGG	1 cut(s)	3337
Bst6I	CTCTTC	5 cut(s)	127, 246, 401, 1454, 1569
BstACI	GRCGYC	4 cut(s)	219, 270, 2097, 3491
BstAFI	CTTAAG	3 cut(s)	650, 1328, 2372
BstAPI	GCANNNNNTGC	3 cut(s)	596, 959, 2411
BstBAI	YACGTR	1 cut(s)	838
BstBI	TTCGAA	2 cut(s)	249, 3091
BstDEI	CTNAG	7 cut(s)	750, 1142, 1673, 2189, 2220, 2357, 3107
BstDSI	CCRYGG	3 cut(s)	357, 3081, 3176
BstF5I	GGATG	7 cut(s)	82, 847, 862, 1489, 1667, 2322, 3247
BstFNI	CGCG	6 cut(s)	52, 275, 387, 678, 2824, 3143
BstH2I	RGCGCY	2 cut(s)	471, 2896
BstHHI	GCGC	6 cut(s)	54, 470, 562, 783, 2895, 3143
BstMAI	GTCTC	2 cut(s)	1208, 2669
BstMCI	CGRYCG	1 cut(s)	279
BstNI	CCWGG	1 cut(s)	3337
BstNSI	RCATGY	2 cut(s)	790, 1246
BstSCI	CCNGG	2 cut(s)	2325, 3335
BstSFI	CTRYAG	7 cut(s)	603, 1614, 2159, 2766, 3042, 3120, 3420
BstSLI	GKGCMC	1 cut(s)	2271
BstUI	CGCG	6 cut(s)	52, 275, 387, 678, 2824, 3143
BstV2I	GAAGAC	5 cut(s)	246, 273, 707, 804, 1207
BstX2I	RGATCY	5 cut(s)	1178, 2155, 2666, 3099, 3424
BstXI	CCANNNNNNTGG	4 cut(s)	1050, 1356, 1541, 2952
BstYI	RGATCY	5 cut(s)	1178, 2155, 2666, 3099, 3424
BstZI	CGGCCG	1 cut(s)	276
Bsu15I	ATCGAT	1 cut(s)	498
Bsu36I	CCTNAGG	1 cut(s)	2189
BsuI	GTATCC	2 cut(s)	1488, 3301
BsuRI	GGCC	5 cut(s)	278, 362, 656, 1737, 2867
BsuTUI	ATCGAT	1 cut(s)	498
BtgI	CCRYGG	3 cut(s)	357, 3081, 3176
BtgZI	GCGATG	1 cut(s)	2106
BtrI	CACGTC	2 cut(s)	2804, 3226
BtsCI	GGATG	7 cut(s)	82, 847, 862, 1489, 1667, 2322, 3247
BtsI	GCAGTG	1 cut(s)	1509
BtsIMutI	CAGTG	5 cut(s)	168, 900, 1043, 1509, 1696
BtuMI	TCGCGA	2 cut(s)	387, 678
BveI	ACCTGC	1 cut(s)	3311
CaiI	CAGNNNCTG	1 cut(s)	596
CciI	TCATGA	1 cut(s)	3366
CfoI	GCGC	6 cut(s)	54, 470, 562, 783, 2895, 3143
Cfr10I	RCCGGY	3 cut(s)	362, 376, 1895
Cfr13I	GGNCC	2 cut(s)	2132, 3505
ClaI	ATCGAT	1 cut(s)	498
CseI	GACGC	4 cut(s)	227, 1022, 2105, 3480
Csp6I	GTAC	9 cut(s)	286, 891, 1040, 1233, 1399, 1698, 2491, 2826, 3213
CviQI	GTAC	9 cut(s)	286, 891, 1040, 1233, 1399, 1698, 2491, 2826, 3213
DdeI	CTNAG	7 cut(s)	750, 1142, 1673, 2189, 2220, 2357, 3107
DraI	TTTAAA	1 cut(s)	3114
DraIII	CACNNNGTG	1 cut(s)	3035
DrdI	GACNNNNNNGTC	1 cut(s)	269
DseDI	GACNNNNNNGTC	1 cut(s)	269
EaeI	YGGCCR	4 cut(s)	276, 360, 1735, 2865
EagI	CGGCCG	1 cut(s)	276
Eam1104I	CTCTTC	5 cut(s)	127, 246, 401, 1454, 1569
EarI	CTCTTC	5 cut(s)	127, 246, 401, 1454, 1569
EciI	GGCGGA	1 cut(s)	178
Ecl136II	GAGCTC	1 cut(s)	1710
EclXI	CGGCCG	1 cut(s)	276
Eco130I	CCWWGG	3 cut(s)	357, 618, 3081
Eco147I	AGGCCT	1 cut(s)	656
Eco24I	GRGCYC	2 cut(s)	321, 1712
Eco32I	GATATC	1 cut(s)	2820
Eco47I	GGWCC	2 cut(s)	2132, 3505
Eco47III	AGCGCT	1 cut(s)	2894
Eco52I	CGGCCG	1 cut(s)	276
Eco53kI	GAGCTC	1 cut(s)	1710
Eco57I	CTGAAG	6 cut(s)	1827, 2424, 2821, 2828, 3177, 3254
Eco72I	CACGTG	1 cut(s)	838
Eco81I	CCTNAGG	1 cut(s)	2189
Eco88I	CYCGRG	4 cut(s)	819, 885, 2574, 2994
EcoICRI	GAGCTC	1 cut(s)	1710
EcoRII	CCWGG	1 cut(s)	3335
EcoRV	GATATC	1 cut(s)	2820
EcoT14I	CCWWGG	3 cut(s)	357, 618, 3081
EcoT38I	GRGCYC	2 cut(s)	321, 1712
ErhI	CCWWGG	3 cut(s)	357, 618, 3081
Esp3I	CGTCTC	1 cut(s)	1208
FalI	AAGNNNNNCTT	8 cut(s)	2028, 2060, 2519, 2551, 2655, 2687, 3241, 3273
FaqI	GGGAC	2 cut(s)	163, 836
FauI	CCCGC	2 cut(s)	205, 3410
FauNDI	CATATG	2 cut(s)	941, 1870
FblI	GTMKAC	1 cut(s)	1549
FokI	GGATG	7 cut(s)	69, 854, 869, 1496, 1654, 2309, 3254
FriOI	GRGCYC	2 cut(s)	321, 1712
FspBI	CTAG	9 cut(s)	113, 1182, 1209, 1248, 1335, 1650, 1974, 1982, 2049
FspI	TGCGCA	1 cut(s)	782
GlaI	GCGC	6 cut(s)	53, 469, 561, 782, 2894, 3142
HaeII	RGCGCY	2 cut(s)	471, 2896
HaeIII	GGCC	5 cut(s)	278, 362, 656, 1737, 2867
HapII	CCGG	7 cut(s)	300, 363, 377, 400, 1481, 1896, 2327
HgaI	GACGC	4 cut(s)	227, 1022, 2105, 3480
HhaI	GCGC	6 cut(s)	54, 470, 562, 783, 2895, 3143
Hin1I	GRCGYC	4 cut(s)	219, 270, 2097, 3491
Hin6I	GCGC	6 cut(s)	52, 468, 560, 781, 2893, 3141
HinP1I	GCGC	6 cut(s)	52, 468, 560, 781, 2893, 3141
HincII	GTYRAC	1 cut(s)	3351
HindII	GTYRAC	1 cut(s)	3351
HindIII	AAGCTT	3 cut(s)	2225, 2369, 2624
HpaII	CCGG	7 cut(s)	300, 363, 377, 400, 1481, 1896, 2327
HphI	GGTGA	7 cut(s)	21, 66, 86, 94, 710, 3047, 3241
Hpy166II	GTNNAC	8 cut(s)	739, 1292, 1550, 1694, 1860, 2392, 2828, 3351
Hpy8I	GTNNAC	8 cut(s)	739, 1292, 1550, 1694, 1860, 2392, 2828, 3351
HpyCH4IV	ACGT	7 cut(s)	270, 837, 1696, 1747, 2803, 2914, 3225
HpyF3I	CTNAG	7 cut(s)	750, 1142, 1673, 2189, 2220, 2357, 3107
HpySE526I	ACGT	7 cut(s)	270, 837, 1696, 1747, 2803, 2914, 3225
Hsp92I	GRCGYC	4 cut(s)	219, 270, 2097, 3491
HspAI	GCGC	6 cut(s)	52, 468, 560, 781, 2893, 3141
Kpn2I	TCCGGA	1 cut(s)	1480
KpnI	GGTACC	1 cut(s)	2494
KroI	GCCGGC	1 cut(s)	376
KroNI	GCCGGC	1 cut(s)	378
LguI	GCTCTTC	1 cut(s)	1569
LmnI	GCTCC	3 cut(s)	191, 1987, 2041
LweI	GCATC	6 cut(s)	736, 1375, 1474, 1676, 2061, 2554
MaeI	CTAG	9 cut(s)	113, 1182, 1209, 1248, 1335, 1650, 1974, 1982, 2049
MaeII	ACGT	7 cut(s)	270, 837, 1696, 1747, 2803, 2914, 3225
MaeIII	GTNAC	8 cut(s)	166, 875, 1018, 1238, 1306, 1512, 1685, 1906
MflI	RGATCY	5 cut(s)	1178, 2155, 2666, 3099, 3424
MhlI	GDGCHC	4 cut(s)	321, 1712, 2271, 2591
MlsI	TGGCCA	1 cut(s)	2867
MluNI	TGGCCA	1 cut(s)	2867
MlyI	GAGTC	4 cut(s)	174, 347, 818, 2642
MmeI	TCCRAC	5 cut(s)	240, 291, 784, 965, 2933
Mox20I	TGGCCA	1 cut(s)	2867
MreI	CGCCGGCG	1 cut(s)	376
MroI	TCCGGA	1 cut(s)	1480
MroNI	GCCGGC	1 cut(s)	376
MroXI	GAANNNNTTC	7 cut(s)	440, 811, 1463, 1565, 1722, 2310, 2960
MscI	TGGCCA	1 cut(s)	2867
MslI	CAYNNNNRTG	2 cut(s)	840, 2861
Msp20I	TGGCCA	1 cut(s)	2867
MspCI	CTTAAG	3 cut(s)	650, 1328, 2372
MspI	CCGG	7 cut(s)	300, 363, 377, 400, 1481, 1896, 2327
MspR9I	CCNGG	2 cut(s)	2327, 3337
Mva1269I	GAATGC	1 cut(s)	527
MvaI	CCWGG	1 cut(s)	3337
MvnI	CGCG	6 cut(s)	52, 275, 387, 678, 2824, 3143
NaeI	GCCGGC	1 cut(s)	378
NciI	CCSGG	1 cut(s)	2327
NcoI	CCATGG	2 cut(s)	357, 3081
NdeI	CATATG	2 cut(s)	941, 1870
NgoMIV	GCCGGC	1 cut(s)	376
NlaIV	GGNNCC	3 cut(s)	1325, 1466, 2492
NmuCI	GTSAC	4 cut(s)	166, 875, 1685, 1906
NruI	TCGCGA	2 cut(s)	387, 678
NsbI	TGCGCA	1 cut(s)	782
NspI	RCATGY	2 cut(s)	790, 1246
NspV	TTCGAA	2 cut(s)	249, 3091
PagI	TCATGA	1 cut(s)	3366
PceI	AGGCCT	1 cut(s)	656
PciI	ACATGT	2 cut(s)	786, 1242
PciSI	GCTCTTC	1 cut(s)	1569
PcsI	WCGNNNNNNNCGW	6 cut(s)	225, 255, 267, 276, 327, 682
PctI	GAATGC	1 cut(s)	527
PdiI	GCCGGC	1 cut(s)	378
PdmI	GAANNNNTTC	7 cut(s)	440, 811, 1463, 1565, 1722, 2310, 2960
PflFI	GACNNNGTC	3 cut(s)	2680, 3464, 3490
PleI	GAGTC	4 cut(s)	173, 347, 818, 2641
PmaCI	CACGTG	1 cut(s)	838
PmlI	CACGTG	1 cut(s)	838
PpsI	GAGTC	4 cut(s)	173, 347, 818, 2641
Ppu21I	YACGTR	1 cut(s)	838
PscI	ACATGT	2 cut(s)	786, 1242
PsiI	TTATAA	2 cut(s)	2526, 2778
Psp124BI	GAGCTC	1 cut(s)	1712
Psp6I	CCWGG	1 cut(s)	3335
PspCI	CACGTG	1 cut(s)	838
PspGI	CCWGG	1 cut(s)	3335
PspN4I	GGNNCC	3 cut(s)	1325, 1466, 2492
PspPI	GGNCC	2 cut(s)	2132, 3505
PstI	CTGCAG	1 cut(s)	3046
PstNI	CAGNNNCTG	1 cut(s)	596
PsuI	RGATCY	5 cut(s)	1178, 2155, 2666, 3099, 3424
PsyI	GACNNNGTC	3 cut(s)	2680, 3464, 3490
RruI	TCGCGA	2 cut(s)	387, 678
RsaI	GTAC	9 cut(s)	287, 892, 1041, 1234, 1400, 1699, 2492, 2827, 3214
RsaNI	GTAC	9 cut(s)	286, 891, 1040, 1233, 1399, 1698, 2491, 2826, 3213
RseI	CAYNNNNRTG	2 cut(s)	840, 2861
SacI	GAGCTC	1 cut(s)	1712
SapI	GCTCTTC	1 cut(s)	1569
Sau96I	GGNCC	2 cut(s)	2132, 3505
ScaI	AGTACT	1 cut(s)	1234
SchI	GAGTC	4 cut(s)	174, 347, 818, 2642
ScrFI	CCNGG	2 cut(s)	2327, 3337
SduI	GDGCHC	4 cut(s)	321, 1712, 2271, 2591
SfaNI	GCATC	6 cut(s)	736, 1375, 1474, 1676, 2061, 2554
SfcI	CTRYAG	7 cut(s)	603, 1614, 2159, 2766, 3042, 3120, 3420
SfuI	TTCGAA	2 cut(s)	249, 3091
SgrAI	CRCCGGYG	1 cut(s)	376
SinI	GGWCC	2 cut(s)	2132, 3505
SmiMI	CAYNNNNRTG	2 cut(s)	840, 2861
SmlI	CTYRAG	8 cut(s)	532, 650, 1302, 1328, 2372, 2556, 2672, 2741
SmoI	CTYRAG	8 cut(s)	532, 650, 1302, 1328, 2372, 2556, 2672, 2741
SseBI	AGGCCT	1 cut(s)	656
SspMI	CTAG	9 cut(s)	113, 1182, 1209, 1248, 1335, 1650, 1974, 1982, 2049
SstI	GAGCTC	1 cut(s)	1712
StuI	AGGCCT	1 cut(s)	656
StyD4I	CCNGG	2 cut(s)	2325, 3335
StyI	CCWWGG	3 cut(s)	357, 618, 3081
TaiI	ACGT	7 cut(s)	273, 840, 1699, 1750, 2806, 2917, 3228
TaqI	TCGA	6 cut(s)	249, 321, 498, 2103, 2926, 3091
TatI	WGTACW	3 cut(s)	1232, 1398, 3212
TauI	GCSGC	4 cut(s)	278, 376, 2468, 3050
TscAI	CASTG	5 cut(s)	175, 900, 1050, 1509, 1696
TseFI	GTSAC	4 cut(s)	166, 875, 1685, 1906
Tsp45I	GTSAC	4 cut(s)	166, 875, 1685, 1906
TspGWI	ACGGA	1 cut(s)	3165
TspRI	CASTG	5 cut(s)	175, 900, 1050, 1509, 1696
Tth111I	GACNNNGTC	3 cut(s)	2680, 3464, 3490
Vha464I	CTTAAG	3 cut(s)	650, 1328, 2372
VpaK11BI	GGWCC	2 cut(s)	2132, 3505
XapI	RAATTY	4 cut(s)	131, 503, 680, 3168
XbaI	TCTAGA	2 cut(s)	1181, 1247
XceI	RCATGY	2 cut(s)	790, 1246
XmiI	GTMKAC	1 cut(s)	1549
XmnI	GAANNNNTTC	7 cut(s)	440, 811, 1463, 1565, 1722, 2310, 2960
XspI	CTAG	9 cut(s)	113, 1182, 1209, 1248, 1335, 1650, 1974, 1982, 2049
ZraI	GACGTC	1 cut(s)	271
ZrmI	AGTACT	1 cut(s)	1234

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.