Rmu_sc0016588.1_g000001

D-ala D-ala ligase N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0016588.1
OG0017819
Physical Location & Seq
Forward (+)
1 .. 5453
5453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0016588.1_g000001.1.cds

Sequence Viewer

Length: 650 bp
ctgtagagaagaagaaagagaaaatggatctgggggtctgcggcactgttggcgagattgagatctcagtcgtggcgtatgtagatcacacattctccattaccgacaaagacataatgatggagacctcctacacagccaacaacaagcctccaatcaaagagatcgctctcgccattgcccttcccgtccttggcattgtcatcggctccctcatggcctataatcgcgtcggcagcggtttcaagtcttggtctgactttgcggaccaccttgctgtcaatgtggacatagtgtttccagtcatacttggtcaatttggtgaagatggtggcattcaggagatcttggaaaggtataacattccatttgttggcactggatcaaatgagtgttatcgagcattcgacaagaccatggcggtcgacggaagaggcctcgacagtgggattgagaatgaaggtgcgacaacgggtgaggtggagctggatcgaacggcttgggtcagcgccagagaggccttgatcgaccggcagtgggccagaggtgtgagaaggtgcttcatctccatggctgtagctcagagaagagccttaccgttttgtgatacaatgcagtttcacagagaatactctctctctctgtgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dala_Dala_lig_C Dala_Dala_lig_N

Protein Analysis

215

Amino Acids

23.75

Weight (kDa)

4.97

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017819)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0004751
rosa_laevigata RLG00000019064
rosa_multiflora Rmu_sc0016588.1_g000001
rosa_roxburghii Rroxscaffold_1G00027510 Rroxscaffold_1G00033930 Rroxscaffold_4G00319660
rosa_samantha Rh2AG035300 Rh2CG363100 Rh4DG036100 Rh7BG463600 Rh7DG253300
rosa_wichuraiana Rw7G020890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 373
AccI GTMKAC 1 cut(s) 425
AccII CGCG 1 cut(s) 230
AciI CCGC 4 cut(s) 41, 239, 265, 421
AclWI GGATC 3 cut(s) 35, 390, 497
AfiI CCNNNNNNNGG 3 cut(s) 193, 373, 537
AgsI TTSAA 1 cut(s) 246
AluBI AGCT 2 cut(s) 486, 580
AluI AGCT 2 cut(s) 486, 580
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 3 cut(s) 35, 390, 497
AoxI GGCC 4 cut(s) 218, 435, 518, 539
ApeKI GCWGC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 511
AspS9I GGNCC 2 cut(s) 267, 539
AsuHPI GGTGA 2 cut(s) 334, 487
AvaII GGWCC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 248
BccI CCATC 2 cut(s) 114, 322
BceAI ACGGC 1 cut(s) 512
BcgI CGANNNNNNTGC 2 cut(s) 186, 220
BcoDI GTCTC 1 cut(s) 118
BfmI CTRYAG 2 cut(s) 2, 575
BfoI RGCGCY 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 517
BglII AGATCT 2 cut(s) 62, 344
BisI GCNGC 2 cut(s) 42, 237
BlsI GCNGC 2 cut(s) 43, 238
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 267, 539
BmiI GGNNCC 1 cut(s) 210
BsaBI GATNNNNATC 1 cut(s) 61
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 192, 416, 569
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 373, 537
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 301, 384
Bse3DI GCAATG 1 cut(s) 176
Bse8I GATNNNNATC 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 192, 416, 569
BseJI GATNNNNATC 1 cut(s) 61
BseLI CCNNNNNNNGG 3 cut(s) 193, 373, 537
BseMI GCAATG 1 cut(s) 176
BseMII CTCAG 2 cut(s) 80, 595
BseNI ACTGG 2 cut(s) 301, 384
BseXI GCAGC 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 230
Bsh1285I CGRYCG 2 cut(s) 425, 531
BshFI GGCC 4 cut(s) 220, 437, 520, 541
BsiEI CGRYCG 2 cut(s) 425, 531
BsiSI CCGG 1 cut(s) 531
BslI CCNNNNNNNGG 3 cut(s) 193, 373, 537
BsmAI GTCTC 1 cut(s) 118
BsmI GAATGC 2 cut(s) 335, 403
BsnI GGCC 4 cut(s) 220, 437, 520, 541
Bso31I GGTCTC 1 cut(s) 118
Bsp143I GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
Bsp19I CCATGG 2 cut(s) 416, 569
BspACI CCGC 4 cut(s) 41, 239, 265, 421
BspANI GGCC 4 cut(s) 220, 437, 520, 541
BspCNI CTCAG 2 cut(s) 79, 594
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 210
BspPI GGATC 3 cut(s) 35, 390, 497
BspQI GCTCTTC 1 cut(s) 582
BspTNI GGTCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 2 cut(s) 301, 384
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 3 cut(s) 192, 416, 569
BssMI GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
BssT1I CCWWGG 3 cut(s) 192, 416, 569
Bst4CI ACNGT 3 cut(s) 48, 445, 599
Bst6I CTCTTC 2 cut(s) 426, 582
BstDEI CTNAG 2 cut(s) 66, 581
BstDSI CCRYGG 2 cut(s) 416, 569
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 1 cut(s) 511
BstKTI GATC 8 cut(s) 30, 65, 87, 167, 347, 385, 492, 527
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
BstMCI CGRYCG 2 cut(s) 425, 531
BstMWI GCNNNNNNNGC 3 cut(s) 50, 236, 517
BstSFI CTRYAG 2 cut(s) 2, 575
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 248
BstX2I RGATCY 3 cut(s) 27, 62, 344
BstYI RGATCY 3 cut(s) 27, 62, 344
BsuRI GGCC 4 cut(s) 220, 437, 520, 541
BtgI CCRYGG 2 cut(s) 416, 569
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 4 cut(s) 44, 377, 450, 541
CfoI GCGC 1 cut(s) 511
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 2 cut(s) 267, 539
CseI GACGC 1 cut(s) 219
CviAII CATG 3 cut(s) 216, 417, 570
DdeI CTNAG 2 cut(s) 66, 581
DpnI GATC 8 cut(s) 29, 64, 86, 166, 346, 384, 491, 526
DpnII GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
Eam1104I CTCTTC 2 cut(s) 426, 582
EarI CTCTTC 2 cut(s) 426, 582
Eco130I CCWWGG 3 cut(s) 192, 416, 569
Eco147I AGGCCT 2 cut(s) 437, 520
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 192, 416, 569
ErhI CCWWGG 3 cut(s) 192, 416, 569
FaeI CATG 3 cut(s) 219, 420, 573
FaiI YATR 9 cut(s) 80, 115, 217, 224, 292, 307, 359, 418, 571
FatI CATG 3 cut(s) 215, 416, 569
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 2 cut(s) 42, 237
Fsp4HI GCNGC 2 cut(s) 42, 237
GlaI GCGC 1 cut(s) 510
GluI GCNGC 2 cut(s) 42, 237
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 4 cut(s) 220, 437, 520, 541
HapII CCGG 1 cut(s) 531
HgaI GACGC 1 cut(s) 219
HhaI GCGC 1 cut(s) 511
Hin1II CATG 3 cut(s) 219, 420, 573
Hin6I GCGC 1 cut(s) 509
HinP1I GCGC 1 cut(s) 509
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HpaII CCGG 1 cut(s) 531
HphI GGTGA 2 cut(s) 334, 487
Hpy166II GTNNAC 2 cut(s) 288, 426
Hpy188I TCNGA 2 cut(s) 258, 584
Hpy188III TCNNGA 1 cut(s) 340
Hpy8I GTNNAC 2 cut(s) 288, 426
Hpy99I CGWCG 2 cut(s) 235, 430
HpyAV CCTTC 3 cut(s) 193, 454, 548
HpyCH4III ACNGT 3 cut(s) 48, 445, 599
HpyCH4V TGCA 1 cut(s) 615
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 236, 517
HpyF3I CTNAG 2 cut(s) 66, 581
Hsp92II CATG 3 cut(s) 219, 420, 573
HspAI GCGC 1 cut(s) 509
Kzo9I GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
LguI GCTCTTC 1 cut(s) 582
LmnI GCTCC 2 cut(s) 214, 483
LpnPI CCDG 8 cut(s) 16, 314, 325, 365, 472, 525, 544, 555
Lsp1109I GCAGC 1 cut(s) 248
MalI GATC 8 cut(s) 29, 64, 86, 166, 346, 384, 491, 526
MboI GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
MboII GAAGA 5 cut(s) 21, 24, 337, 443, 599
MflI RGATCY 3 cut(s) 27, 62, 344
MluCI AATT 1 cut(s) 316
MnlI CCTC 8 cut(s) 138, 161, 223, 427, 448, 471, 510, 538
MseI TTAA 1 cut(s) 648
MslI CAYNNNNRTG 2 cut(s) 118, 568
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 531
Mva1269I GAATGC 2 cut(s) 335, 403
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 50, 236, 517
NcoI CCATGG 2 cut(s) 416, 569
NdeII GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
NlaIII CATG 3 cut(s) 219, 420, 573
NlaIV GGNNCC 1 cut(s) 210
PceI AGGCCT 2 cut(s) 437, 520
PciSI GCTCTTC 1 cut(s) 582
PctI GAATGC 2 cut(s) 335, 403
PflMI CCANNNNNTGG 1 cut(s) 373
PkrI GCNGC 2 cut(s) 43, 238
PspN4I GGNNCC 1 cut(s) 210
PspPI GGNCC 2 cut(s) 267, 539
PsuI RGATCY 3 cut(s) 27, 62, 344
RseI CAYNNNNRTG 2 cut(s) 118, 568
SalI GTCGAC 1 cut(s) 424
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 2 cut(s) 42, 237
Sau3AI GATC 8 cut(s) 27, 62, 84, 164, 344, 382, 489, 524
Sau96I GGNCC 2 cut(s) 267, 539
SetI ASST 9 cut(s) 130, 275, 358, 465, 482, 488, 549, 559, 582
SfcI CTRYAG 2 cut(s) 2, 575
SinI GGWCC 1 cut(s) 267
SmiMI CAYNNNNRTG 2 cut(s) 118, 568
Sse9I AATT 1 cut(s) 316
SseBI AGGCCT 2 cut(s) 437, 520
SsiI CCGC 4 cut(s) 41, 239, 265, 421
StuI AGGCCT 2 cut(s) 437, 520
StyI CCWWGG 3 cut(s) 192, 416, 569
TaaI ACNGT 3 cut(s) 48, 445, 599
TaqI TCGA 6 cut(s) 399, 407, 425, 440, 492, 527
TasI AATT 1 cut(s) 316
TauI GCSGC 1 cut(s) 44
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 4 cut(s) 51, 384, 450, 541
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 2 cut(s) 473, 552
TspGWI ACGGA 1 cut(s) 443
TspRI CASTG 4 cut(s) 51, 384, 450, 541
Van91I CCANNNNNTGG 1 cut(s) 373
VpaK11BI GGWCC 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.