Rmu_ssc0000189.1_g000043

Flavin containing amine oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000189.1
OG0016601
Physical Location & Seq
Reverse (-)
276989 .. 282217
5229 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2400 bp
atgttcactgtcctatgtggcaaccccgagtcttctttcacgtctgacagacggacgtcagttggtgatataatctataatttgaaaggttgtggagcttgtgcttcacaagtgcacaccgttgctgctttctcatggctgctaattaaagaggcacttgagaacactgagccttatcaattgtctactgtggcggcaatgtcctcattcccaaatactctgcagtttctccaacctctttctcacgctagcaccaaatccctcagaaaatcagttttggcgcgaaactcttcaaatggctctgtctccctcaaacaaatcaaaactaccccactcccaggcaaagcagaggctgatgttgtagtcattgggagtggtataggtggattgtgttgtgccgggctattagctaggtaccagcaagatgtccttgtcttagagagtcatgatctacccggcggtgctgctcattcatttgagattaaaggctttcaatttgactccggcccatctttgactgtgagcttgagacggacgtcagttggtgatttaatctataatttgaaaggttgtggagcttgtgcttcacgagtgcacaccgttgctgctttctcatggctgccaattaaagaggcacttgagaacactgagccttatcaattgtcaactgtggcggcaatgtcctcgttcccaaatactctgcagtttctccaacctctttctcacgctagcaccaaatccctcagaaaatcagttttggcgcgaaactcttcaaatggctctgtctccctcaaacaaatcaaaactaccccactcccaggcaaagcagaggctgatgttgtagtcattgggagtggtataggtggattgtgttgtgccgggctattagctaggtaccagcaagatgtccttgtcttagagagtcatgatctacccggcggtgctgctcattcatttgagattaaaggctttcaatttgactccggcccatctttgttctctggttttcaatccaaaggccctcaggccaatcctcttggacaagttctggatgcattgggtgagacaattccttgtgccagttatgattcatggatggtctacctacctgaaggtgaatttctgtcgcgcattggtcccacagactttttcaaggaccttgagaagtatgcaagtgcaaatgctgttcaagaatggaaaaagcttttggatgccattcttccaatgtctgcagctgcaatggctctgcctccactttctatccgaggggatttgggtgttctttctactgctggagctagatatgctccctctctattaaaatcttttgctcaaatgggacctaagggagcgcttggtgccacaaagctactaagaccattttcagaaatcatggactcattagaactgaaagatccctttatacgtaattgggtggatctactggctttcttgcttgctggggtgaaatctaatggtatactttcagcagagatggtttatatgtttgcggagtggtataagccaggttgctgtcttgaataccctcttcatggaagtggtgcagttgttgaagctcttgttcggggaatgcagaagtttggtggaaggatttctttaggaagtcatgtggaaaaaattgttgttgaaaacggtcgagccactggggtcaagctaagaagtggacaatttatacgtgctaagaaggctgttgttagtaatgcatctatgtgggacactctaaatctattacctaaagaaattgttccaaagtcatacgagaaaaggattgagatgacccctcaatgtgaatccttcatgcatcttcatttgggatttgatgctgagtgtacgcataaagacttaggaattcatcatatagttgtcaatgattgggatagaggggttgatgctgaccagaatgttgttttgatatctgtaccaagtgtactaagtccggatctggcaccacctgggaaacatgtcttgcatgcctatcttccaggaactgaaccctttgatttgtggaaaggattggaccgtaaaagtactgcatacaaagagctcaaagctgaaagatccgaggttatgtggagagctgtggagcgggcacttggtcctgggtttagccgtgaaaaatgcgaggtcaagttagttggttctccattgacgcatcaaagatttcttcgaaggaaccgaggaacatatgggccagcagtagaagctggtaaagactctattcctgggcactccactccgattcctcaactttattgctgtggagattctacttttcctggcattggagtccctgcagttgctgctagtggtgccattgttgccaattcactagtttctgttgctcaacactctcaacttcttgatgcaattggaatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

799

Amino Acids

85.76

Weight (kDa)

8.18

Isoelectric Point (pI)

35.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 119 - 156 4.4e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 122 - 173 5.7e-13 NAD(P)-binding Rossmann-like domain
DAO PF01266 279 - 316 5.2e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 282 - 337 1.2e-14 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 287 - 782 2.6e-21 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016601)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57770
fragaria_vesca FvH4_5g28280
malus_domestica MD08G1243700.v1.1
prunus_persica Prupe.1G581000_v2.0.a1 Prupe.1G581000_v2.0.a1 Prupe.1G581000_v2.0.a1
pyrus_communis pycom08g21140
rosa_chinensis RchiOBHm_Chr7g0220531
rosa_laevigata RLG00000002219
rosa_multiflora Rmu_ssc0000189.1_g000043
rosa_roxburghii Rroxscaffold_3G00239320
rosa_rugosa Rorug07G0194400
rosa_samantha Rh7DG332000
rosa_wichuraiana Rw7G028590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 59, 539
Acc65I GGTACC 2 cut(s) 414, 894
AccB1I GGYRCC 5 cut(s) 414, 894, 1358, 1966, 2330
AccBSI CCGCTC 1 cut(s) 2107
AccI GTMKAC 3 cut(s) 185, 1101, 1480
AccII CGCG 3 cut(s) 283, 763, 1129
AccIII TCCGGA 1 cut(s) 1957
AciI CCGC 6 cut(s) 194, 459, 674, 939, 1511, 2107
AclWI GGATC 4 cut(s) 1409, 1446, 1968, 2073
AcsI RAATTY 2 cut(s) 1118, 1869
AcuI CTGAAG 1 cut(s) 1131
AcyI GRCGYC 2 cut(s) 56, 536
AfaI GTAC 6 cut(s) 416, 896, 1852, 1941, 1950, 2050
AfeI AGCGCT 1 cut(s) 1353
AfiI CCNNNNNNNGG 4 cut(s) 338, 818, 1553, 2303
AflIII ACRYGT 1 cut(s) 1981
AhlI ACTAGT 1 cut(s) 2350
AjiI CACGTC 1 cut(s) 42
AjnI CCWGG 8 cut(s) 337, 817, 1525, 1972, 2002, 2119, 2242, 2296
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
Alw21I GWGCWC 3 cut(s) 117, 597, 2067
Alw26I GTCTC 4 cut(s) 310, 523, 790, 1058
Alw44I GTGCAC 2 cut(s) 113, 593
AlwI GGATC 4 cut(s) 1409, 1446, 1968, 2073
AlwNI CAGNNNCTG 4 cut(s) 353, 833, 2009, 2321
Ama87I CYCGRG 1 cut(s) 26
Aor13HI TCCGGA 1 cut(s) 1957
Aor51HI AGCGCT 1 cut(s) 1353
AoxI GGCC 5 cut(s) 505, 985, 1018, 1026, 2210
ApaLI GTGCAC 2 cut(s) 113, 593
ApeKI GCWGC 9 cut(s) 125, 139, 464, 605, 619, 944, 1232, 1235, 2321
ApoI RAATTY 2 cut(s) 1118, 1869
Asp700I GAANNNNTTC 4 cut(s) 289, 769, 1612, 1764
Asp718I GGTACC 2 cut(s) 414, 894
AspLEI GCGC 4 cut(s) 283, 763, 1131, 1354
AspS9I GGNCC 9 cut(s) 506, 986, 1019, 1136, 1156, 1340, 2038, 2117, 2210
AsuC2I CCSGG 4 cut(s) 400, 456, 880, 936
AsuHPI GGTGA 5 cut(s) 77, 557, 1073, 1127, 1477
AsuII TTCGAA 1 cut(s) 2188
AsuNHI GCTAGC 2 cut(s) 248, 728
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 5 cut(s) 1136, 1156, 1340, 2038, 2117
AxyI CCTNAGG 2 cut(s) 1023, 1344
BaeGI GKGCMC 4 cut(s) 117, 597, 2113, 2250
BanI GGYRCC 5 cut(s) 414, 894, 1358, 1966, 2330
BanII GRGCYC 1 cut(s) 2067
BauI CACGAG 1 cut(s) 588
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 3 cut(s) 117, 597, 2067
BbvI GCAGC 9 cut(s) 112, 126, 451, 592, 606, 931, 1222, 1244, 2308
BccI CCATC 4 cut(s) 517, 997, 1090, 1489
BceAI ACGGC 1 cut(s) 2115
BcgI CGANNNNNNTGC 2 cut(s) 666, 700
BciT130I CCWGG 8 cut(s) 339, 819, 1527, 1974, 2004, 2121, 2244, 2298
BcnI CCSGG 4 cut(s) 400, 456, 880, 936
BcoDI GTCTC 4 cut(s) 310, 523, 790, 1058
BcuI ACTAGT 1 cut(s) 2350
BfaI CTAG 7 cut(s) 249, 411, 729, 891, 1299, 2325, 2351
BfmI CTRYAG 4 cut(s) 221, 701, 1230, 2313
BfoI RGCGCY 1 cut(s) 1355
BmcAI AGTACT 1 cut(s) 2050
Bme18I GGWCC 5 cut(s) 1136, 1156, 1340, 2038, 2117
BmeT110I CYCGRG 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 42
BmgT120I GGNCC 9 cut(s) 506, 986, 1019, 1136, 1156, 1340, 2038, 2117, 2210
BmiI GGNNCC 8 cut(s) 416, 896, 1138, 1341, 1360, 1968, 2195, 2332
BmrI ACTGGG 1 cut(s) 1674
BmsI GCATC 8 cut(s) 1042, 1201, 1733, 1831, 1831, 1900, 2182, 2374
BmtI GCTAGC 2 cut(s) 252, 732
BmuI ACTGGG 1 cut(s) 1674
BoxI GACNNNNGTC 2 cut(s) 55, 535
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 1314
Bpu14I TTCGAA 1 cut(s) 2188
BpuEI CTTGAG 4 cut(s) 179, 547, 659, 1181
BpuMI CCSGG 4 cut(s) 400, 456, 880, 936
BsaAI YACGTR 2 cut(s) 1427, 1697
BsaHI GRCGYC 2 cut(s) 56, 536
BsaJI CCNNGG 8 cut(s) 337, 817, 1264, 1973, 2082, 2120, 2197, 2243
BsaWI WCCGGW 1 cut(s) 1957
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 818, 1553, 2303
Bse1I ACTGG 3 cut(s) 1080, 1449, 1669
Bse21I CCTNAGG 2 cut(s) 1023, 1344
Bse3DI GCAATG 3 cut(s) 204, 684, 1245
BseAI TCCGGA 1 cut(s) 1957
BseBI CCWGG 8 cut(s) 339, 819, 1527, 1974, 2004, 2121, 2244, 2298
BseDI CCNNGG 8 cut(s) 337, 817, 1264, 1973, 2082, 2120, 2197, 2243
BseGI GGATG 3 cut(s) 1057, 1101, 1216
BseLI CCNNNNNNNGG 4 cut(s) 338, 818, 1553, 2303
BseMI GCAATG 3 cut(s) 204, 684, 1245
BseMII CTCAG 6 cut(s) 159, 277, 639, 757, 1037, 1836
BseNI ACTGG 3 cut(s) 1080, 1449, 1669
BseSI GKGCMC 4 cut(s) 117, 597, 2113, 2250
BseXI GCAGC 9 cut(s) 112, 126, 451, 592, 606, 931, 1222, 1244, 2308
BseYI CCCAGC 1 cut(s) 1460
BsgI GTGCAG 1 cut(s) 1584
Bsh1236I CGCG 3 cut(s) 283, 763, 1129
Bsh1285I CGRYCG 1 cut(s) 1657
BshFI GGCC 5 cut(s) 507, 987, 1020, 1028, 2212
BshNI GGYRCC 5 cut(s) 414, 894, 1358, 1966, 2330
BsiEI CGRYCG 1 cut(s) 1657
BsiHKAI GWGCWC 3 cut(s) 117, 597, 2067
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 7 cut(s) 399, 456, 504, 879, 936, 984, 1958
BslFI GGGAC 4 cut(s) 1122, 1353, 1748, 2294
BslI CCNNNNNNNGG 4 cut(s) 338, 818, 1553, 2303
BsmAI GTCTC 4 cut(s) 310, 523, 790, 1058
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 4 cut(s) 1122, 1353, 1748, 2294
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 5 cut(s) 507, 987, 1020, 1028, 2212
BsoBI CYCGRG 1 cut(s) 26
Bsp119I TTCGAA 1 cut(s) 2188
Bsp1286I GDGCHC 5 cut(s) 117, 597, 2067, 2113, 2250
Bsp13I TCCGGA 1 cut(s) 1957
Bsp143I GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
BspACI CCGC 6 cut(s) 194, 459, 674, 939, 1511, 2107
BspANI GGCC 5 cut(s) 507, 987, 1020, 1028, 2212
BspCNI CTCAG 6 cut(s) 160, 276, 640, 756, 1036, 1837
BspEI TCCGGA 1 cut(s) 1957
BspFNI CGCG 3 cut(s) 283, 763, 1129
BspHI TCATGA 2 cut(s) 445, 925
BspLI GGNNCC 8 cut(s) 416, 896, 1138, 1341, 1360, 1968, 2195, 2332
BspMAI CTGCAG 4 cut(s) 225, 705, 1234, 2317
BspOI GCTAGC 2 cut(s) 252, 732
BspPI GGATC 4 cut(s) 1409, 1446, 1968, 2073
BspT104I TTCGAA 1 cut(s) 2188
BspT107I GGYRCC 5 cut(s) 414, 894, 1358, 1966, 2330
BsrBI CCGCTC 1 cut(s) 2107
BsrDI GCAATG 3 cut(s) 204, 684, 1245
BsrI ACTGG 3 cut(s) 1080, 1449, 1669
BssECI CCNNGG 8 cut(s) 337, 817, 1264, 1973, 2082, 2120, 2197, 2243
BssMI GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
BssNAI GTATAC 1 cut(s) 1481
BssNI GRCGYC 2 cut(s) 56, 536
BssSI CACGAG 1 cut(s) 588
Bst1107I GTATAC 1 cut(s) 1481
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 8 cut(s) 339, 819, 1527, 1974, 2004, 2121, 2244, 2298
Bst4CI ACNGT 8 cut(s) 10, 121, 190, 520, 601, 670, 1655, 2042
Bst6I CTCTTC 3 cut(s) 295, 775, 1554
BstACI GRCGYC 2 cut(s) 56, 536
BstAPI GCANNNNNTGC 1 cut(s) 2321
BstBAI YACGTR 2 cut(s) 1427, 1697
BstBI TTCGAA 1 cut(s) 2188
BstC8I GCNNGC 6 cut(s) 250, 730, 1458, 1992, 2109, 2214
BstF5I GGATG 3 cut(s) 1057, 1101, 1216
BstFNI CGCG 3 cut(s) 283, 763, 1129
BstH2I RGCGCY 1 cut(s) 1355
BstHHI GCGC 4 cut(s) 283, 763, 1131, 1354
BstKTI GATC 6 cut(s) 451, 931, 1417, 1441, 1963, 2081
BstMAI GTCTC 4 cut(s) 310, 523, 790, 1058
BstMBI GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
BstMCI CGRYCG 1 cut(s) 1657
BstMWI GCNNNNNNNGC 8 cut(s) 1241, 1304, 1358, 1706, 2222, 2321, 2330, 2339
BstNI CCWGG 8 cut(s) 339, 819, 1527, 1974, 2004, 2121, 2244, 2298
BstNSI RCATGY 2 cut(s) 1985, 1994
BstPAI GACNNNNGTC 2 cut(s) 55, 535
BstSFI CTRYAG 4 cut(s) 221, 701, 1230, 2313
BstSLI GKGCMC 4 cut(s) 117, 597, 2113, 2250
BstSNI TACGTA 1 cut(s) 1427
BstUI CGCG 3 cut(s) 283, 763, 1129
BstV1I GCAGC 9 cut(s) 112, 126, 451, 592, 606, 931, 1222, 1244, 2308
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 4 cut(s) 1414, 1438, 1960, 2078
BstYI RGATCY 4 cut(s) 1414, 1438, 1960, 2078
BstZ17I GTATAC 1 cut(s) 1481
Bsu36I CCTNAGG 2 cut(s) 1023, 1344
BsuRI GGCC 5 cut(s) 507, 987, 1020, 1028, 2212
BtrI CACGTC 1 cut(s) 42
BtsCI GGATG 3 cut(s) 1057, 1101, 1216
BtsIMutI CAGTG 4 cut(s) 6, 165, 645, 1662
Cac8I GCNNGC 6 cut(s) 250, 730, 1458, 1992, 2109, 2214
CaiI CAGNNNCTG 4 cut(s) 353, 833, 2009, 2321
CciI TCATGA 2 cut(s) 445, 925
CfoI GCGC 4 cut(s) 283, 763, 1131, 1354
Cfr13I GGNCC 9 cut(s) 506, 986, 1019, 1136, 1156, 1340, 2038, 2117, 2210
CseI GACGC 1 cut(s) 2179
Csp6I GTAC 6 cut(s) 415, 895, 1851, 1940, 1949, 2049
CviQI GTAC 6 cut(s) 415, 895, 1851, 1940, 1949, 2049
DpnI GATC 6 cut(s) 450, 930, 1416, 1440, 1962, 2080
DpnII GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
Eam1104I CTCTTC 3 cut(s) 295, 775, 1554
EarI CTCTTC 3 cut(s) 295, 775, 1554
Ecl136II GAGCTC 1 cut(s) 2065
Eco105I TACGTA 1 cut(s) 1427
Eco24I GRGCYC 1 cut(s) 2067
Eco32I GATATC 1 cut(s) 1935
Eco47I GGWCC 5 cut(s) 1136, 1156, 1340, 2038, 2117
Eco47III AGCGCT 1 cut(s) 1353
Eco53kI GAGCTC 1 cut(s) 2065
Eco57I CTGAAG 1 cut(s) 1131
Eco81I CCTNAGG 2 cut(s) 1023, 1344
Eco88I CYCGRG 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 2065
EcoO109I RGGNCCY 3 cut(s) 1019, 1156, 1340
EcoRI GAATTC 1 cut(s) 1869
EcoRII CCWGG 8 cut(s) 337, 817, 1525, 1972, 2002, 2119, 2242, 2296
EcoRV GATATC 1 cut(s) 1935
EcoT22I ATGCAT 3 cut(s) 1057, 1726, 1824
EcoT38I GRGCYC 1 cut(s) 2067
Esp3I CGTCTC 1 cut(s) 523
FaqI GGGAC 4 cut(s) 1122, 1353, 1748, 2294
FauI CCCGC 1 cut(s) 2100
FauNDI CATATG 1 cut(s) 2206
FblI GTMKAC 3 cut(s) 185, 1101, 1480
FokI GGATG 3 cut(s) 1064, 1108, 1223
FriOI GRGCYC 1 cut(s) 2067
FspBI CTAG 7 cut(s) 249, 411, 729, 891, 1299, 2325, 2351
GlaI GCGC 4 cut(s) 282, 762, 1130, 1353
GsaI CCCAGC 1 cut(s) 1464
GsuI CTGGAG 1 cut(s) 1314
HaeII RGCGCY 1 cut(s) 1355
HaeIII GGCC 5 cut(s) 507, 987, 1020, 1028, 2212
HapII CCGG 7 cut(s) 399, 456, 504, 879, 936, 984, 1958
HgaI GACGC 1 cut(s) 2179
HhaI GCGC 4 cut(s) 283, 763, 1131, 1354
Hin1I GRCGYC 2 cut(s) 56, 536
Hin6I GCGC 4 cut(s) 281, 761, 1129, 1352
HinP1I GCGC 4 cut(s) 281, 761, 1129, 1352
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 1202
HpaII CCGG 7 cut(s) 399, 456, 504, 879, 936, 984, 1958
HphI GGTGA 5 cut(s) 77, 557, 1073, 1127, 1477
Hpy188I TCNGA 8 cut(s) 46, 266, 746, 1265, 1387, 2083, 2259, 2399
Hpy188III TCNNGA 8 cut(s) 446, 588, 926, 1049, 1190, 1538, 1958, 2381
HpyAV CCTTC 5 cut(s) 1106, 1602, 1699, 1825, 2184
HpyCH4III ACNGT 8 cut(s) 10, 121, 190, 520, 601, 670, 1655, 2042
HpyCH4IV ACGT 5 cut(s) 41, 56, 536, 1426, 1696
HpyF10VI GCNNNNNNNGC 8 cut(s) 1241, 1304, 1358, 1706, 2222, 2321, 2330, 2339
HpySE526I ACGT 5 cut(s) 41, 56, 536, 1426, 1696
Hsp92I GRCGYC 2 cut(s) 56, 536
HspAI GCGC 4 cut(s) 281, 761, 1129, 1352
Kpn2I TCCGGA 1 cut(s) 1957
KpnI GGTACC 2 cut(s) 418, 898
Kzo9I GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
LmnI GCTCC 6 cut(s) 95, 575, 1295, 1312, 1349, 2104
Lsp1109I GCAGC 9 cut(s) 112, 126, 451, 592, 606, 931, 1222, 1244, 2308
LweI GCATC 8 cut(s) 1042, 1201, 1733, 1831, 1831, 1900, 2182, 2374
MaeI CTAG 7 cut(s) 249, 411, 729, 891, 1299, 2325, 2351
MaeII ACGT 5 cut(s) 41, 56, 536, 1426, 1696
MalI GATC 6 cut(s) 450, 930, 1416, 1440, 1962, 2080
MbiI CCGCTC 1 cut(s) 2107
MboI GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
MboII GAAGA 8 cut(s) 24, 282, 762, 1211, 1541, 1817, 1991, 2177
MfeI CAATTG 3 cut(s) 179, 659, 2388
MflI RGATCY 4 cut(s) 1414, 1438, 1960, 2078
MhlI GDGCHC 5 cut(s) 117, 597, 2067, 2113, 2250
MlyI GAGTC 8 cut(s) 38, 451, 494, 931, 974, 1391, 2228, 2316
MmeI TCCRAC 2 cut(s) 256, 736
Mph1103I ATGCAT 3 cut(s) 1057, 1726, 1824
MroI TCCGGA 1 cut(s) 1957
MroXI GAANNNNTTC 4 cut(s) 289, 769, 1612, 1764
MseI TTAA 6 cut(s) 147, 483, 551, 627, 963, 1319
MslI CAYNNNNRTG 2 cut(s) 1557, 1729
MspA1I CMGCKG 1 cut(s) 1235
MspI CCGG 7 cut(s) 399, 456, 504, 879, 936, 984, 1958
MunI CAATTG 3 cut(s) 179, 659, 2388
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 8 cut(s) 339, 819, 1527, 1974, 2004, 2121, 2244, 2298
MvnI CGCG 3 cut(s) 283, 763, 1129
MwoI GCNNNNNNNGC 8 cut(s) 1241, 1304, 1358, 1706, 2222, 2321, 2330, 2339
NciI CCSGG 4 cut(s) 400, 456, 880, 936
NdeI CATATG 1 cut(s) 2206
NdeII GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
NheI GCTAGC 2 cut(s) 248, 728
NlaIV GGNNCC 8 cut(s) 416, 896, 1138, 1341, 1360, 1968, 2195, 2332
NsiI ATGCAT 3 cut(s) 1057, 1726, 1824
NspI RCATGY 2 cut(s) 1985, 1994
NspV TTCGAA 1 cut(s) 2188
PaeI GCATGC 1 cut(s) 1994
PagI TCATGA 2 cut(s) 445, 925
PciI ACATGT 1 cut(s) 1981
PcsI WCGNNNNNNNCGW 1 cut(s) 2194
PctI GAATGC 1 cut(s) 1596
PdmI GAANNNNTTC 4 cut(s) 289, 769, 1612, 1764
PfeI GAWTC 5 cut(s) 1088, 1811, 2260, 2285, 2394
PfoI TCCNGGA 1 cut(s) 2002
PleI GAGTC 8 cut(s) 37, 450, 494, 930, 974, 1391, 2228, 2315
PpsI GAGTC 8 cut(s) 37, 450, 494, 930, 974, 1391, 2228, 2315
Ppu21I YACGTR 2 cut(s) 1427, 1697
PpuMI RGGWCCY 2 cut(s) 1156, 1340
PscI ACATGT 1 cut(s) 1981
PshAI GACNNNNGTC 2 cut(s) 55, 535
Psp124BI GAGCTC 1 cut(s) 2067
Psp5II RGGWCCY 2 cut(s) 1156, 1340
Psp6I CCWGG 8 cut(s) 337, 817, 1525, 1972, 2002, 2119, 2242, 2296
PspFI CCCAGC 1 cut(s) 1460
PspGI CCWGG 8 cut(s) 337, 817, 1525, 1972, 2002, 2119, 2242, 2296
PspN4I GGNNCC 8 cut(s) 416, 896, 1138, 1341, 1360, 1968, 2195, 2332
PspPI GGNCC 9 cut(s) 506, 986, 1019, 1136, 1156, 1340, 2038, 2117, 2210
PspPPI RGGWCCY 2 cut(s) 1156, 1340
PstI CTGCAG 4 cut(s) 225, 705, 1234, 2317
PstNI CAGNNNCTG 4 cut(s) 353, 833, 2009, 2321
PsuI RGATCY 4 cut(s) 1414, 1438, 1960, 2078
PvuII CAGCTG 1 cut(s) 1235
RsaI GTAC 6 cut(s) 416, 896, 1852, 1941, 1950, 2050
RsaNI GTAC 6 cut(s) 415, 895, 1851, 1940, 1949, 2049
RseI CAYNNNNRTG 2 cut(s) 1557, 1729
SacI GAGCTC 1 cut(s) 2067
SaqAI TTAA 6 cut(s) 147, 483, 551, 627, 963, 1319
Sau3AI GATC 6 cut(s) 448, 928, 1414, 1438, 1960, 2078
Sau96I GGNCC 9 cut(s) 506, 986, 1019, 1136, 1156, 1340, 2038, 2117, 2210
ScaI AGTACT 1 cut(s) 2050
SchI GAGTC 8 cut(s) 38, 451, 494, 931, 974, 1391, 2228, 2316
SduI GDGCHC 5 cut(s) 117, 597, 2067, 2113, 2250
SfaNI GCATC 8 cut(s) 1042, 1201, 1733, 1831, 1831, 1900, 2182, 2374
SfcI CTRYAG 4 cut(s) 221, 701, 1230, 2313
SfuI TTCGAA 1 cut(s) 2188
SinI GGWCC 5 cut(s) 1136, 1156, 1340, 2038, 2117
SmiMI CAYNNNNRTG 2 cut(s) 1557, 1729
SmlI CTYRAG 4 cut(s) 158, 526, 638, 1160
SmoI CTYRAG 4 cut(s) 158, 526, 638, 1160
SnaBI TACGTA 1 cut(s) 1427
SpeI ACTAGT 1 cut(s) 2350
SphI GCATGC 1 cut(s) 1994
SsiI CCGC 6 cut(s) 194, 459, 674, 939, 1511, 2107
SspMI CTAG 7 cut(s) 249, 411, 729, 891, 1299, 2325, 2351
SstI GAGCTC 1 cut(s) 2067
TaaI ACNGT 8 cut(s) 10, 121, 190, 520, 601, 670, 1655, 2042
TaiI ACGT 5 cut(s) 44, 59, 539, 1429, 1699
TaqI TCGA 2 cut(s) 1657, 2188
TatI WGTACW 2 cut(s) 1948, 2048
TauI GCSGC 2 cut(s) 197, 677
TfiI GAWTC 5 cut(s) 1088, 1811, 2260, 2285, 2394
Tru1I TTAA 6 cut(s) 147, 483, 551, 627, 963, 1319
Tru9I TTAA 6 cut(s) 147, 483, 551, 627, 963, 1319
TscAI CASTG 4 cut(s) 13, 172, 652, 1669
TseI GCWGC 9 cut(s) 125, 139, 464, 605, 619, 944, 1232, 1235, 2321
TspDTI ATGAA 7 cut(s) 462, 942, 1080, 1541, 1807, 1817, 1862
TspGWI ACGGA 2 cut(s) 67, 547
TspRI CASTG 4 cut(s) 13, 172, 652, 1669
VneI GTGCAC 2 cut(s) 113, 593
VpaK11BI GGWCC 5 cut(s) 1136, 1156, 1340, 2038, 2117
XapI RAATTY 2 cut(s) 1118, 1869
XceI RCATGY 2 cut(s) 1985, 1994
XmiI GTMKAC 3 cut(s) 185, 1101, 1480
XmnI GAANNNNTTC 4 cut(s) 289, 769, 1612, 1764
XspI CTAG 7 cut(s) 249, 411, 729, 891, 1299, 2325, 2351
ZraI GACGTC 2 cut(s) 57, 537
ZrmI AGTACT 1 cut(s) 2050
Zsp2I ATGCAT 3 cut(s) 1057, 1726, 1824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.