Rroxscaffold_3G00239320

Flavin containing amine oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016601
Physical Location & Seq
Reverse (-)
29451949 .. 29457590
5642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00239320.1

Sequence Viewer

Length: 2181 bp
ATGGCTGCTGCAAGGGCCTCCGCCAGTTTGATTTGCTGCTGCAACTCAAGAGCCTTGATAGGCTTGATCAAGAGAATAGAACAAGACCTGCCCTTGAGAACAGGGACCTTCAGAGCAGAGCAATCTTATCCTCATGGCCCTGCATTACCTAATCAATCGGGAGGCCTTGTTGACCTTGCGCATGCAAAGCCTGTGACGAATCAGCTCACTGAAATGACTCTTGGTCCTTGGGTGGATGAAATCATAGAGCTGAAACATACAGATCCAGAAACGCAAGCACCAGAAACGATCACAAACGTCGGTCAAGACAAAGCATACCTTGACTGTTGTGGAGCTTGTGCTTCACAAGTGCACACGGTTGCTGCTTTCTCATGGCTGCTAATCAAAGAGGCACTCGAGAACACTGAGCCTTATCAATTGTCAACTGTGGCGGCAATGTCCTCATTCCCAAATACTCTGCAGTTTCTCCAACCTCTTTCTCACACTAGCACCAAATCCCTCAGAAAATCAGTTTTGGCGCGAAACTCTTCAAATGGCTCTGTCTCCCTCAAACAAATCAAAACTACCCCATTCCCAGGCAAAGCAGAGGCTGATGTTGTAGTCATTGGGAGTGGTATAGGTGGATTGTGTTGTGCCGGGCTATTAGCTAGGTATAAGCAAGATGTCCTTGTCTTAGAGAGCCATGATCTACCCGGCGGTGCTGCTCATTCATTTGAGATTAAAGGCTTTCAATTTGACTCCGGCCCATCTTTGTTCTCTGGTTTTCAATCCAAAGGCCCTCAGGCCAATCCTCTTGGACAAGTTCTGGATGCATTGGGTGAGACAATTCCTTGTGCCAGTTATGATTCATGGATGGTCTACCTACCTGAAGGTGAATTTCTGTCGCGCATTGGTCCCACAGACTTTTTCAAGGTCATTGTTAATCCTCTAGGAGTAGACCTTGAGAAGTATGCAAGTGCAAATGCTGTTCAAGAATGGAAAAAGCTTTTGGATGCCATTCTTCCAATGTCTGCAGCTGCGATGGCTCTGCCTCCACTTTCTATCCGAGGGGATTTGGGCGTTCTTTCTACTGCTGGAGCTAGATATGCTCCCTCTCTATTAAAATCTTTTGCTCAAATGGGACCTAAGGGAGCGCTTGGTGCCACAAAGCTACTAAGACCATTTTCAGAAATCATGGACTCATTAGAACTGAAAGATCCCTTTATACGTAATTGGGTGGATCTACTGGCTTTCTTGCTTGCTGGGGTGAAATCTAATGGTATACTTTCAGCAGAGATGGTTTATATGTTTGCGGAGTGGTATAAGCCAGGTTGCTGTCTTGAATACCCTCTTCATGGAAGTGGTGCAGTTGTTGAAGCTCTTGTTCGGGGAATGCAGAAGTTTGGTGGAAGGATTTCTTTAGGAAGTCATGTGGAAAAAATTGTTGTTGAAAACGGTCGAGCCACTGGGGTCAAGCTAAGAAGTGGACAATTTATACGTGCTAAGAAGGCTGTTGTTAGTAATGCATCTATGTGGGACACTCTAAATCTATTACCTAAAGAAGTTGTTCCAAAGTCATACGAGAAAAGGATTGAGATGACCCCTCAATGTGAATCCTTCATGCATCTTCATTTGGGATTTGATGCTGAGTGTACGCATAAAGACTTAGGAATTCATCATATAGTTGTCAATGATTGGGATAGAGGGGTTGATGCTGACCAGAATGTTGTTTTGATATCTGTACCAAGTGTACTTAGTCCGGATCTGGCACCACCTGGGAAACATGTTTTGCATGCCTATCTTCCAGGAACTGAACCCTTTAATTTGTGGAAAGGATTGGACCGTAAAAGTGCTGCATACAAAGAGCTCAAAGCTGAAAGATCAGAGGTTATGTGGAGAGCTGTGGAGCGGGCGCTTGGTCCTGGGTTTAGCCGTGAAAAATGCGAGGTCAAGTTAGTTGGTTCTCCATTGACGCATCAAAGGTTTCTTCGAAGGAACCGAGGAACATATGGACCAGCAGTAGAAGCTGGTAAAGACTCTATTCCTGGGCACTCCACTCCGATTCCTCAACTTTATTGCTGTGGAGATTCTACTTTTCCTGGCATTGGAGTCCCTGCAGTTGCTGCTAGTGGTGCCATTGTTGCCAATTCACTAGTTTCTGTTGCTCAACACTCTCAACTTCTTGATGCAATTGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Amino_oxidase NAD_binding_8

Protein Analysis

726

Amino Acids

78.2

Weight (kDa)

7.02

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 198 - 235 3.7e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 201 - 256 8.7e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 206 - 709 3.9e-21 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016601)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G57770
fragaria_vesca FvH4_5g28280
malus_domestica MD08G1243700.v1.1
prunus_persica Prupe.1G581000_v2.0.a1 Prupe.1G581000_v2.0.a1 Prupe.1G581000_v2.0.a1
pyrus_communis pycom08g21140
rosa_chinensis RchiOBHm_Chr7g0220531
rosa_laevigata RLG00000002219
rosa_multiflora Rmu_ssc0000189.1_g000043
rosa_roxburghii Rroxscaffold_3G00239320
rosa_rugosa Rorug07G0194400
rosa_samantha Rh7DG332000
rosa_wichuraiana Rw7G028590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 96
AccB1I GGYRCC 3 cut(s) 1139, 1747, 2111
AccBSI CCGCTC 1 cut(s) 1888
AccI GTMKAC 3 cut(s) 858, 936, 1261
AccII CGCG 2 cut(s) 520, 886
AccIII TCCGGA 1 cut(s) 1738
AciI CCGC 5 cut(s) 21, 431, 696, 1292, 1888
AclWI GGATC 4 cut(s) 257, 1190, 1227, 1749
AcsI RAATTY 2 cut(s) 875, 1650
AcuI CTGAAG 2 cut(s) 94, 888
AfaI GTAC 3 cut(s) 1633, 1722, 1731
AfeI AGCGCT 1 cut(s) 1134
AfiI CCNNNNNNNGG 3 cut(s) 575, 1334, 2084
AflIII ACRYGT 1 cut(s) 1762
AgsI TTSAA 8 cut(s) 531, 731, 767, 910, 971, 1322, 1355, 1430
AhdI GACNNNNNGTC 1 cut(s) 222
AhlI ACTAGT 1 cut(s) 2131
AjnI CCWGG 7 cut(s) 574, 1306, 1753, 1783, 1900, 2023, 2077
AjuI GAANNNNNNNTTGG 4 cut(s) 204, 236, 971, 1003
Alw21I GWGCWC 2 cut(s) 354, 1848
Alw26I GTCTC 2 cut(s) 547, 815
Alw44I GTGCAC 1 cut(s) 350
AlwI GGATC 4 cut(s) 257, 1190, 1227, 1749
AlwNI CAGNNNCTG 3 cut(s) 590, 1790, 2102
Ama87I CYCGRG 1 cut(s) 395
Aor13HI TCCGGA 1 cut(s) 1738
Aor51HI AGCGCT 1 cut(s) 1134
AoxI GGCC 6 cut(s) 15, 136, 163, 742, 775, 783
ApaLI GTGCAC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 875, 1650
Asp700I GAANNNNTTC 3 cut(s) 526, 1393, 1545
AspLEI GCGC 5 cut(s) 181, 520, 888, 1135, 1894
AsuC2I CCSGG 2 cut(s) 637, 693
AsuHPI GGTGA 3 cut(s) 830, 884, 1258
AsuII TTCGAA 1 cut(s) 1969
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 7 cut(s) 105, 224, 893, 1121, 1819, 1898, 1991
AxyI CCTNAGG 2 cut(s) 780, 1125
BaeGI GKGCMC 2 cut(s) 354, 2031
BanI GGYRCC 3 cut(s) 1139, 1747, 2111
BanII GRGCYC 1 cut(s) 1848
Bbv12I GWGCWC 2 cut(s) 354, 1848
BbvI GCAGC 9 cut(s) 23, 26, 349, 363, 688, 1003, 1025, 1819, 2089
BccI CCATC 4 cut(s) 754, 847, 1015, 1270
BceAI ACGGC 1 cut(s) 1896
BcgI CGANNNNNNTGC 2 cut(s) 1009, 1043
BciT130I CCWGG 7 cut(s) 576, 1308, 1755, 1785, 1902, 2025, 2079
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 2 cut(s) 637, 693
BcoDI GTCTC 2 cut(s) 547, 815
BcuI ACTAGT 1 cut(s) 2131
BfaI CTAG 6 cut(s) 486, 648, 929, 1080, 2106, 2132
BfmI CTRYAG 3 cut(s) 458, 1011, 2094
BfoI RGCGCY 2 cut(s) 1136, 1895
BfuAI ACCTGC 1 cut(s) 96
Bme1390I CCNGG 9 cut(s) 576, 637, 693, 1308, 1755, 1785, 1902, 2025, 2079
Bme18I GGWCC 7 cut(s) 105, 224, 893, 1121, 1819, 1898, 1991
BmeRI GACNNNNNGTC 1 cut(s) 222
BmeT110I CYCGRG 1 cut(s) 395
BmiI GGNNCC 7 cut(s) 106, 895, 1122, 1141, 1749, 1976, 2113
BmrFI CCNGG 9 cut(s) 576, 637, 693, 1308, 1755, 1785, 1902, 2025, 2079
BmrI ACTGGG 1 cut(s) 1455
BmsI GCATC 8 cut(s) 799, 982, 1514, 1612, 1612, 1681, 1963, 2155
BmuI ACTGGG 1 cut(s) 1455
BpmI CTGGAG 1 cut(s) 1095
Bpu14I TTCGAA 1 cut(s) 1969
BpuEI CTTGAG 3 cut(s) 31, 115, 962
BpuMI CCSGG 2 cut(s) 637, 693
BsaAI YACGTR 2 cut(s) 1208, 1478
BsaJI CCNNGG 7 cut(s) 227, 574, 1045, 1754, 1901, 1978, 2024
BsaWI WCCGGW 1 cut(s) 1738
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 1334, 2084
Bse1I ACTGG 4 cut(s) 24, 837, 1230, 1450
Bse21I CCTNAGG 2 cut(s) 780, 1125
Bse3DI GCAATG 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 1738
BseBI CCWGG 7 cut(s) 576, 1308, 1755, 1785, 1902, 2025, 2079
BseDI CCNNGG 7 cut(s) 227, 574, 1045, 1754, 1901, 1978, 2024
BseGI GGATG 4 cut(s) 241, 814, 858, 997
BseLI CCNNNNNNNGG 3 cut(s) 575, 1334, 2084
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 4 cut(s) 396, 514, 794, 1617
BseNI ACTGG 4 cut(s) 24, 837, 1230, 1450
BseSI GKGCMC 2 cut(s) 354, 2031
BseXI GCAGC 9 cut(s) 23, 26, 349, 363, 688, 1003, 1025, 1819, 2089
BseYI CCCAGC 1 cut(s) 1241
BsgI GTGCAG 1 cut(s) 1365
Bsh1236I CGCG 2 cut(s) 520, 886
Bsh1285I CGRYCG 1 cut(s) 1438
BshFI GGCC 6 cut(s) 17, 138, 165, 744, 777, 785
BshNI GGYRCC 3 cut(s) 1139, 1747, 2111
BsiEI CGRYCG 1 cut(s) 1438
BsiHKAI GWGCWC 2 cut(s) 354, 1848
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 4 cut(s) 636, 693, 741, 1739
BslFI GGGAC 5 cut(s) 118, 879, 1134, 1529, 2075
BslI CCNNNNNNNGG 3 cut(s) 575, 1334, 2084
BsmAI GTCTC 2 cut(s) 547, 815
BsmFI GGGAC 5 cut(s) 118, 879, 1134, 1529, 2075
BsmI GAATGC 1 cut(s) 1377
BsnI GGCC 6 cut(s) 17, 138, 165, 744, 777, 785
BsoBI CYCGRG 1 cut(s) 395
Bsp119I TTCGAA 1 cut(s) 1969
Bsp1286I GDGCHC 3 cut(s) 354, 1848, 2031
Bsp13I TCCGGA 1 cut(s) 1738
Bsp143I GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
BspACI CCGC 5 cut(s) 21, 431, 696, 1292, 1888
BspANI GGCC 6 cut(s) 17, 138, 165, 744, 777, 785
BspCNI CTCAG 4 cut(s) 397, 513, 793, 1618
BspEI TCCGGA 1 cut(s) 1738
BspFNI CGCG 2 cut(s) 520, 886
BspLI GGNNCC 7 cut(s) 106, 895, 1122, 1141, 1749, 1976, 2113
BspMAI CTGCAG 3 cut(s) 462, 1015, 2098
BspMI ACCTGC 1 cut(s) 96
BspPI GGATC 4 cut(s) 257, 1190, 1227, 1749
BspT104I TTCGAA 1 cut(s) 1969
BspT107I GGYRCC 3 cut(s) 1139, 1747, 2111
BsrBI CCGCTC 1 cut(s) 1888
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 4 cut(s) 24, 837, 1230, 1450
BssECI CCNNGG 7 cut(s) 227, 574, 1045, 1754, 1901, 1978, 2024
BssMI GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
BssNAI GTATAC 1 cut(s) 1262
BssT1I CCWWGG 1 cut(s) 227
Bst1107I GTATAC 1 cut(s) 1262
Bst2UI CCWGG 7 cut(s) 576, 1308, 1755, 1785, 1902, 2025, 2079
Bst4CI ACNGT 5 cut(s) 326, 358, 427, 1436, 1823
Bst6I CTCTTC 2 cut(s) 532, 1335
BstAPI GCANNNNNTGC 1 cut(s) 2102
BstBAI YACGTR 2 cut(s) 1208, 1478
BstBI TTCGAA 1 cut(s) 1969
BstC8I GCNNGC 5 cut(s) 183, 276, 1239, 1773, 1890
BstF5I GGATG 4 cut(s) 241, 814, 858, 997
BstFNI CGCG 2 cut(s) 520, 886
BstH2I RGCGCY 2 cut(s) 1136, 1895
BstHHI GCGC 5 cut(s) 181, 520, 888, 1135, 1894
BstKTI GATC 8 cut(s) 69, 265, 291, 688, 1198, 1222, 1744, 1862
BstMAI GTCTC 2 cut(s) 547, 815
BstMBI GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
BstMCI CGRYCG 1 cut(s) 1438
BstNI CCWGG 7 cut(s) 576, 1308, 1755, 1785, 1902, 2025, 2079
BstNSI RCATGY 3 cut(s) 185, 1766, 1775
BstSCI CCNGG 9 cut(s) 574, 635, 691, 1306, 1753, 1783, 1900, 2023, 2077
BstSFI CTRYAG 3 cut(s) 458, 1011, 2094
BstSLI GKGCMC 2 cut(s) 354, 2031
BstSNI TACGTA 1 cut(s) 1208
BstUI CGCG 2 cut(s) 520, 886
BstV1I GCAGC 9 cut(s) 23, 26, 349, 363, 688, 1003, 1025, 1819, 2089
BstX2I RGATCY 4 cut(s) 262, 1195, 1219, 1741
BstYI RGATCY 4 cut(s) 262, 1195, 1219, 1741
BstZ17I GTATAC 1 cut(s) 1262
Bsu36I CCTNAGG 2 cut(s) 780, 1125
BsuRI GGCC 6 cut(s) 17, 138, 165, 744, 777, 785
BtgZI GCGATG 1 cut(s) 1034
BtsCI GGATG 4 cut(s) 241, 814, 858, 997
BtsIMutI CAGTG 3 cut(s) 207, 402, 1443
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 5 cut(s) 183, 276, 1239, 1773, 1890
CaiI CAGNNNCTG 3 cut(s) 590, 1790, 2102
CfoI GCGC 5 cut(s) 181, 520, 888, 1135, 1894
CseI GACGC 1 cut(s) 1960
Csp6I GTAC 3 cut(s) 1632, 1721, 1730
CviQI GTAC 3 cut(s) 1632, 1721, 1730
DpnI GATC 8 cut(s) 68, 264, 290, 687, 1197, 1221, 1743, 1861
DpnII GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
DriI GACNNNNNGTC 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 532, 1335
Eam1105I GACNNNNNGTC 1 cut(s) 222
EarI CTCTTC 2 cut(s) 532, 1335
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 1846
Eco105I TACGTA 1 cut(s) 1208
Eco130I CCWWGG 1 cut(s) 227
Eco147I AGGCCT 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 1848
Eco32I GATATC 1 cut(s) 1716
Eco47I GGWCC 7 cut(s) 105, 224, 893, 1121, 1819, 1898, 1991
Eco47III AGCGCT 1 cut(s) 1134
Eco53kI GAGCTC 1 cut(s) 1846
Eco57I CTGAAG 2 cut(s) 94, 888
Eco81I CCTNAGG 2 cut(s) 780, 1125
Eco88I CYCGRG 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 1846
EcoO109I RGGNCCY 4 cut(s) 15, 105, 776, 1121
EcoRI GAATTC 1 cut(s) 1650
EcoRII CCWGG 7 cut(s) 574, 1306, 1753, 1783, 1900, 2023, 2077
EcoRV GATATC 1 cut(s) 1716
EcoT14I CCWWGG 1 cut(s) 227
EcoT22I ATGCAT 3 cut(s) 814, 1507, 1605
EcoT38I GRGCYC 1 cut(s) 1848
ErhI CCWWGG 1 cut(s) 227
FalI AAGNNNNNCTT 6 cut(s) 303, 335, 651, 683, 1381, 1413
FaqI GGGAC 5 cut(s) 118, 879, 1134, 1529, 2075
FauI CCCGC 1 cut(s) 1881
FauNDI CATATG 1 cut(s) 1987
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 3 cut(s) 858, 936, 1261
FokI GGATG 4 cut(s) 248, 821, 865, 1004
FriOI GRGCYC 1 cut(s) 1848
FspBI CTAG 6 cut(s) 486, 648, 929, 1080, 2106, 2132
FspI TGCGCA 1 cut(s) 180
GlaI GCGC 5 cut(s) 180, 519, 887, 1134, 1893
GsaI CCCAGC 1 cut(s) 1245
GsuI CTGGAG 1 cut(s) 1095
HaeII RGCGCY 2 cut(s) 1136, 1895
HaeIII GGCC 6 cut(s) 17, 138, 165, 744, 777, 785
HapII CCGG 4 cut(s) 636, 693, 741, 1739
HgaI GACGC 1 cut(s) 1960
HhaI GCGC 5 cut(s) 181, 520, 888, 1135, 1894
Hin6I GCGC 5 cut(s) 179, 518, 886, 1133, 1892
HinP1I GCGC 5 cut(s) 179, 518, 886, 1133, 1892
HincII GTYRAC 2 cut(s) 172, 423
HindII GTYRAC 2 cut(s) 172, 423
HindIII AAGCTT 1 cut(s) 983
HpaII CCGG 4 cut(s) 636, 693, 741, 1739
HphI GGTGA 3 cut(s) 830, 884, 1258
Hpy166II GTNNAC 9 cut(s) 172, 352, 423, 859, 937, 1262, 1466, 1632, 1730
Hpy188I TCNGA 7 cut(s) 113, 503, 1046, 1168, 1864, 2040, 2180
Hpy8I GTNNAC 9 cut(s) 172, 352, 423, 859, 937, 1262, 1466, 1632, 1730
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 6 cut(s) 118, 863, 1383, 1480, 1606, 1965
HpyCH4III ACNGT 5 cut(s) 326, 358, 427, 1436, 1823
HpyCH4IV ACGT 3 cut(s) 297, 1207, 1477
HpySE526I ACGT 3 cut(s) 297, 1207, 1477
HspAI GCGC 5 cut(s) 179, 518, 886, 1133, 1892
Kpn2I TCCGGA 1 cut(s) 1738
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
LmnI GCTCC 5 cut(s) 332, 1076, 1093, 1130, 1885
Lsp1109I GCAGC 9 cut(s) 23, 26, 349, 363, 688, 1003, 1025, 1819, 2089
LweI GCATC 8 cut(s) 799, 982, 1514, 1612, 1612, 1681, 1963, 2155
MaeI CTAG 6 cut(s) 486, 648, 929, 1080, 2106, 2132
MaeII ACGT 3 cut(s) 297, 1207, 1477
MaeIII GTNAC 1 cut(s) 193
MalI GATC 8 cut(s) 68, 264, 290, 687, 1197, 1221, 1743, 1861
MbiI CCGCTC 1 cut(s) 1888
MboI GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
MboII GAAGA 6 cut(s) 519, 992, 1322, 1598, 1772, 1958
MfeI CAATTG 2 cut(s) 416, 2169
MflI RGATCY 4 cut(s) 262, 1195, 1219, 1741
MhlI GDGCHC 3 cut(s) 354, 1848, 2031
MlyI GAGTC 5 cut(s) 211, 731, 1172, 2009, 2097
MmeI TCCRAC 1 cut(s) 493
Mph1103I ATGCAT 3 cut(s) 814, 1507, 1605
MroI TCCGGA 1 cut(s) 1738
MroXI GAANNNNTTC 3 cut(s) 526, 1393, 1545
MseI TTAA 4 cut(s) 720, 921, 1100, 1800
MslI CAYNNNNRTG 3 cut(s) 212, 1338, 1510
MspA1I CMGCKG 1 cut(s) 1016
MspI CCGG 4 cut(s) 636, 693, 741, 1739
MspR9I CCNGG 9 cut(s) 576, 637, 693, 1308, 1755, 1785, 1902, 2025, 2079
MunI CAATTG 2 cut(s) 416, 2169
Mva1269I GAATGC 1 cut(s) 1377
MvaI CCWGG 7 cut(s) 576, 1308, 1755, 1785, 1902, 2025, 2079
MvnI CGCG 2 cut(s) 520, 886
NciI CCSGG 2 cut(s) 637, 693
NdeI CATATG 1 cut(s) 1987
NdeII GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
NlaIV GGNNCC 7 cut(s) 106, 895, 1122, 1141, 1749, 1976, 2113
NmuCI GTSAC 1 cut(s) 193
NsbI TGCGCA 1 cut(s) 180
NsiI ATGCAT 3 cut(s) 814, 1507, 1605
NspI RCATGY 3 cut(s) 185, 1766, 1775
NspV TTCGAA 1 cut(s) 1969
PaeI GCATGC 2 cut(s) 185, 1775
PaeR7I CTCGAG 1 cut(s) 395
PceI AGGCCT 1 cut(s) 165
PciI ACATGT 1 cut(s) 1762
PcsI WCGNNNNNNNCGW 1 cut(s) 1975
PctI GAATGC 1 cut(s) 1377
PdmI GAANNNNTTC 3 cut(s) 526, 1393, 1545
PfeI GAWTC 6 cut(s) 199, 845, 1592, 2041, 2066, 2175
PfoI TCCNGGA 1 cut(s) 1783
PleI GAGTC 5 cut(s) 211, 731, 1172, 2009, 2096
PpsI GAGTC 5 cut(s) 211, 731, 1172, 2009, 2096
Ppu21I YACGTR 2 cut(s) 1208, 1478
PpuMI RGGWCCY 2 cut(s) 105, 1121
PscI ACATGT 1 cut(s) 1762
Psp124BI GAGCTC 1 cut(s) 1848
Psp5II RGGWCCY 2 cut(s) 105, 1121
Psp6I CCWGG 7 cut(s) 574, 1306, 1753, 1783, 1900, 2023, 2077
PspFI CCCAGC 1 cut(s) 1241
PspGI CCWGG 7 cut(s) 574, 1306, 1753, 1783, 1900, 2023, 2077
PspN4I GGNNCC 7 cut(s) 106, 895, 1122, 1141, 1749, 1976, 2113
PspPPI RGGWCCY 2 cut(s) 105, 1121
PstI CTGCAG 3 cut(s) 462, 1015, 2098
PstNI CAGNNNCTG 3 cut(s) 590, 1790, 2102
PsuI RGATCY 4 cut(s) 262, 1195, 1219, 1741
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 3 cut(s) 1633, 1722, 1731
RsaNI GTAC 3 cut(s) 1632, 1721, 1730
RseI CAYNNNNRTG 3 cut(s) 212, 1338, 1510
SacI GAGCTC 1 cut(s) 1848
SaqAI TTAA 4 cut(s) 720, 921, 1100, 1800
Sau3AI GATC 8 cut(s) 66, 262, 288, 685, 1195, 1219, 1741, 1859
SchI GAGTC 5 cut(s) 211, 731, 1172, 2009, 2097
ScrFI CCNGG 9 cut(s) 576, 637, 693, 1308, 1755, 1785, 1902, 2025, 2079
SduI GDGCHC 3 cut(s) 354, 1848, 2031
SfaNI GCATC 8 cut(s) 799, 982, 1514, 1612, 1612, 1681, 1963, 2155
SfcI CTRYAG 3 cut(s) 458, 1011, 2094
Sfr274I CTCGAG 1 cut(s) 395
SfuI TTCGAA 1 cut(s) 1969
SinI GGWCC 7 cut(s) 105, 224, 893, 1121, 1819, 1898, 1991
SlaI CTCGAG 1 cut(s) 395
SmiMI CAYNNNNRTG 3 cut(s) 212, 1338, 1510
SmlI CTYRAG 4 cut(s) 46, 94, 395, 941
SmoI CTYRAG 4 cut(s) 46, 94, 395, 941
SnaBI TACGTA 1 cut(s) 1208
SpeI ACTAGT 1 cut(s) 2131
SphI GCATGC 2 cut(s) 185, 1775
SseBI AGGCCT 1 cut(s) 165
SsiI CCGC 5 cut(s) 21, 431, 696, 1292, 1888
SspMI CTAG 6 cut(s) 486, 648, 929, 1080, 2106, 2132
SstI GAGCTC 1 cut(s) 1848
StuI AGGCCT 1 cut(s) 165
StyD4I CCNGG 9 cut(s) 574, 635, 691, 1306, 1753, 1783, 1900, 2023, 2077
StyI CCWWGG 1 cut(s) 227
TaaI ACNGT 5 cut(s) 326, 358, 427, 1436, 1823
TaiI ACGT 3 cut(s) 300, 1210, 1480
TaqI TCGA 3 cut(s) 396, 1438, 1969
TaqII GACCGA 1 cut(s) 290
TatI WGTACW 1 cut(s) 1729
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 6 cut(s) 199, 845, 1592, 2041, 2066, 2175
Tru1I TTAA 4 cut(s) 720, 921, 1100, 1800
Tru9I TTAA 4 cut(s) 720, 921, 1100, 1800
TscAI CASTG 3 cut(s) 214, 409, 1450
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 7 cut(s) 252, 699, 837, 1322, 1588, 1598, 1643
TspRI CASTG 3 cut(s) 214, 409, 1450
VneI GTGCAC 1 cut(s) 350
VpaK11BI GGWCC 7 cut(s) 105, 224, 893, 1121, 1819, 1898, 1991
XapI RAATTY 2 cut(s) 875, 1650
XceI RCATGY 3 cut(s) 185, 1766, 1775
XhoI CTCGAG 1 cut(s) 395
XmiI GTMKAC 3 cut(s) 858, 936, 1261
XmnI GAANNNNTTC 3 cut(s) 526, 1393, 1545
XspI CTAG 6 cut(s) 486, 648, 929, 1080, 2106, 2132
Zsp2I ATGCAT 3 cut(s) 814, 1507, 1605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.