Rmu_ssc0000388.1_g000047

MatE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000388.1
OG0016752
Physical Location & Seq
Reverse (-)
230057 .. 235416
5360 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1620 bp
atggcgctccattcccacactactcacctccaaacccataagcgcttatcgcacccttcaatcttgtacaacccacttcacacctctcaccgcagctctgttcgttacgccgttaagccgcgaagttttggtgctttggtggcgaaaagcgagcgtggcaatggacggctgataacggcgtgcattgctggcggtaatgctgacgcgtttgaagaggaagtggtggtggaggtcgtgggttcgaagaaggaggagttggtggagcaaagcctatggaagcaggtgaaggacattgtcatgttttcgggtccggccaccgggttgtggatttgtgggccgttgatgagtctcatctccaccgctgttatcggccaggctagctctactgagcttgctgctttgggaccgggaactgtcttctgtgacaacatgaatcttttgttcatgttcctttcgattgcgacttctaatatggttgccactgcgcttgccaaaagggataaaaaggaactgcagcatcagatatctatattgctcttcgttggattgacttgtggagttctgatgctcttgtttacacaattccttggttcatgggcgctaacagcttttgctgggcccaagaatgtgcatattatacctgcagcaagcaaatatgttcagattcgggggttagcgtggcctgcgattctttttggattggttgctcaaagcgcaagtcttggcatgaaagattcattgggacctttaaaagctctggtggttgccagtgttgtgaatggccttggtcatgtagtcctatgcaacatgttacactatggtattactggtgcagcatgggcaacaatggcatcgcagattgttgctgcttgtatgatgattgaagctctgaacaagaaaggatttagtgcattttccatcactgttccatcgcctcgtgaatgtctgctaatatttgggattgcaggtccagtgtttgtaacaatgttttcaaaggtggccttctactcactgatgacatactttgctactgctatgggtacaaatatagttgccgctcatcaggtcatgatccaaatgtatgggatgtgcgttgtatggggtgagcctctttctcaaactgcacaatcgtttatgcctgagttattatacggagttaaccgaagtttggagaaggcccgaatgctgctaaagtcactcgtaattattggagctattcttggattggcaataggggttattgggatgtctgttccttggttgttccccaatatctttacatttgacctgaaagtcatacaagagatgcacaaagtattgtttctgttcttcctgtcgttgtctattacgccctgtactcacagccttgagggcacattgctggctggacgtgatcttaccttcatcagcttgtcaatgagtagttgtttttccttgggtgctcttctactgttgcttgtgaacagcagaggatttggtttgccaggctgctggtttgcgcttgttggctttcaatgggctcgatttttcctagctctccgacggcttctttctcccaatggcatgctccattcagaagatctgggctcgtataaagtggggaagttgaaagctacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

539

Amino Acids

58.29

Weight (kDa)

9.31

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 111 - 260 3.1e-15 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016752)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10120 FvH4_2g10120 FvH4_2g10120 FvH4_2g10120
rosa_chinensis RchiOBHm_Chr6g0267191
rosa_laevigata RLG00000014020
rosa_multiflora Rmu_sc0000908.1_g000024 Rmu_ssc0000388.1_g000047
rosa_roxburghii Rroxscaffold_7G00201000
rosa_rugosa Rorug06G0030400
rosa_samantha Rh6BG152600 Rh6CG148500 Rh6DG136800
rosa_wichuraiana Rw6G013140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 271
AasI GACNNNNNNGTC 1 cut(s) 1292
Acc36I ACCTGC 3 cut(s) 271, 649, 956
AccBSI CCGCTC 1 cut(s) 1058
AccII CGCG 2 cut(s) 121, 206
AciI CCGC 5 cut(s) 91, 119, 192, 360, 1056
AclWI GGATC 1 cut(s) 1066
AcoI YGGCCR 2 cut(s) 312, 370
AfaI GTAC 3 cut(s) 68, 1042, 1357
AfeI AGCGCT 1 cut(s) 44
AfiI CCNNNNNNNGG 3 cut(s) 317, 324, 1168
AflIII ACRYGT 2 cut(s) 204, 807
AgsI TTSAA 6 cut(s) 60, 212, 884, 993, 1514, 1609
AjiI CACGTC 1 cut(s) 1391
AjnI CCWGG 2 cut(s) 372, 1483
AjuI GAANNNNNNNTTGG 4 cut(s) 239, 271, 722, 754
Alw21I GWGCWC 1 cut(s) 1444
Alw26I GTCTC 1 cut(s) 353
AlwI GGATC 1 cut(s) 1066
Aor51HI AGCGCT 1 cut(s) 44
AoxI GGCC 8 cut(s) 312, 335, 370, 617, 680, 781, 999, 1176
ApaI GGGCCC 1 cut(s) 621
ApeKI GCWGC 8 cut(s) 93, 395, 514, 644, 833, 866, 1186, 1488
ArsI GACNNNNNNTTYG 2 cut(s) 703, 735
AspLEI GCGC 6 cut(s) 7, 45, 487, 601, 716, 1501
AspS9I GGNCC 8 cut(s) 308, 335, 404, 617, 618, 743, 968, 1177
AsuC2I CCSGG 2 cut(s) 319, 408
AsuHPI GGTGA 4 cut(s) 17, 80, 295, 1115
AsuII TTCGAA 1 cut(s) 242
AsuNHI GCTAGC 1 cut(s) 377
AvaII GGWCC 4 cut(s) 308, 404, 743, 968
BaeGI GKGCMC 2 cut(s) 621, 1376
BanII GRGCYC 3 cut(s) 621, 1522, 1589
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 1444
BbvI GCAGC 8 cut(s) 105, 382, 526, 656, 845, 853, 1173, 1475
BccI CCATC 2 cut(s) 926, 937
BceAI ACGGC 5 cut(s) 95, 182, 192, 322, 1559
BciT130I CCWGG 2 cut(s) 374, 1485
BcnI CCSGG 2 cut(s) 319, 408
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 2 cut(s) 378, 1532
BfmI CTRYAG 2 cut(s) 512, 642
BfoI RGCGCY 3 cut(s) 8, 46, 602
BfuAI ACCTGC 3 cut(s) 271, 649, 956
BglI GCCNNNNNGGC 1 cut(s) 1371
BglII AGATCT 1 cut(s) 1579
Bme1390I CCNGG 4 cut(s) 319, 374, 408, 1485
Bme18I GGWCC 4 cut(s) 308, 404, 743, 968
BmgBI CACGTC 1 cut(s) 1391
BmgT120I GGNCC 8 cut(s) 308, 335, 404, 617, 618, 743, 968, 1177
BmiI GGNNCC 4 cut(s) 309, 405, 619, 744
BmrFI CCNGG 4 cut(s) 319, 374, 408, 1485
BmsI GCATC 4 cut(s) 526, 555, 860, 1296
BmtI GCTAGC 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 409
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 1388
BpuMI CCSGG 2 cut(s) 319, 408
BsaJI CCNNGG 4 cut(s) 586, 784, 1256, 1434
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 324, 1168
Bse1I ACTGG 3 cut(s) 768, 832, 971
Bse3DI GCAATG 3 cut(s) 166, 183, 1376
BseBI CCWGG 2 cut(s) 374, 1485
BseDI CCNNGG 4 cut(s) 586, 784, 1256, 1434
BseGI GGATG 2 cut(s) 1092, 1251
BseLI CCNNNNNNNGG 3 cut(s) 317, 324, 1168
BseMI GCAATG 3 cut(s) 166, 183, 1376
BseMII CTCAG 2 cut(s) 378, 1131
BseNI ACTGG 3 cut(s) 768, 832, 971
BseRI GAGGAG 1 cut(s) 266
BseSI GKGCMC 2 cut(s) 621, 1376
BseXI GCAGC 8 cut(s) 105, 382, 526, 656, 845, 853, 1173, 1475
BseYI CCCAGC 1 cut(s) 614
BsgI GTGCAG 2 cut(s) 852, 1107
Bsh1236I CGCG 2 cut(s) 121, 206
BshFI GGCC 8 cut(s) 314, 337, 372, 619, 682, 783, 1001, 1178
BsiHKAI GWGCWC 1 cut(s) 1444
BsiSI CCGG 3 cut(s) 311, 318, 407
BslFI GGGAC 2 cut(s) 417, 756
BslI CCNNNNNNNGG 3 cut(s) 317, 324, 1168
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 2 cut(s) 417, 756
BsmI GAATGC 1 cut(s) 1188
BsnI GGCC 8 cut(s) 314, 337, 372, 619, 682, 783, 1001, 1178
Bsp119I TTCGAA 1 cut(s) 242
Bsp120I GGGCCC 1 cut(s) 617
Bsp1286I GDGCHC 5 cut(s) 621, 1376, 1444, 1522, 1589
Bsp1407I TGTACA 1 cut(s) 66
Bsp143I GATC 3 cut(s) 1071, 1393, 1579
BspACI CCGC 5 cut(s) 91, 119, 192, 360, 1056
BspANI GGCC 8 cut(s) 314, 337, 372, 619, 682, 783, 1001, 1178
BspCNI CTCAG 2 cut(s) 379, 1132
BspFNI CGCG 2 cut(s) 121, 206
BspHI TCATGA 1 cut(s) 1068
BspLI GGNNCC 4 cut(s) 309, 405, 619, 744
BspMAI CTGCAG 2 cut(s) 516, 646
BspMI ACCTGC 3 cut(s) 271, 649, 956
BspOI GCTAGC 1 cut(s) 381
BspPI GGATC 1 cut(s) 1066
BspQI GCTCTTC 2 cut(s) 542, 1449
BspT104I TTCGAA 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 1058
BsrDI GCAATG 3 cut(s) 166, 183, 1376
BsrGI TGTACA 1 cut(s) 66
BsrI ACTGG 3 cut(s) 768, 832, 971
BssECI CCNNGG 4 cut(s) 586, 784, 1256, 1434
BssMI GATC 3 cut(s) 1071, 1393, 1579
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 4 cut(s) 586, 784, 1256, 1434
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 2 cut(s) 374, 1485
Bst4CI ACNGT 3 cut(s) 415, 925, 1452
Bst6I CTCTTC 3 cut(s) 207, 542, 1449
BstAUI TGTACA 1 cut(s) 66
BstBI TTCGAA 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 387, 1140
BstF5I GGATG 2 cut(s) 1092, 1251
BstFNI CGCG 2 cut(s) 121, 206
BstH2I RGCGCY 3 cut(s) 8, 46, 602
BstHHI GCGC 6 cut(s) 7, 45, 487, 601, 716, 1501
BstKTI GATC 3 cut(s) 1074, 1396, 1582
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 3 cut(s) 1071, 1393, 1579
BstNI CCWGG 2 cut(s) 374, 1485
BstNSI RCATGY 2 cut(s) 811, 1567
BstSCI CCNGG 4 cut(s) 317, 372, 406, 1483
BstSFI CTRYAG 2 cut(s) 512, 642
BstSLI GKGCMC 2 cut(s) 621, 1376
BstUI CGCG 2 cut(s) 121, 206
BstV1I GCAGC 8 cut(s) 105, 382, 526, 656, 845, 853, 1173, 1475
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 1579
BstXI CCANNNNNNTGG 2 cut(s) 1082, 1491
BstYI RGATCY 1 cut(s) 1579
BsuRI GGCC 8 cut(s) 314, 337, 372, 619, 682, 783, 1001, 1178
BtgZI GCGATG 2 cut(s) 837, 915
BtrI CACGTC 1 cut(s) 1391
BtsCI GGATG 2 cut(s) 1092, 1251
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 5 cut(s) 480, 775, 921, 978, 1010
BveI ACCTGC 3 cut(s) 271, 649, 956
CciI TCATGA 1 cut(s) 1068
CfoI GCGC 6 cut(s) 7, 45, 487, 601, 716, 1501
Cfr13I GGNCC 8 cut(s) 308, 335, 404, 617, 618, 743, 968, 1177
CseI GACGC 1 cut(s) 212
Csp6I GTAC 3 cut(s) 67, 1041, 1356
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviQI GTAC 3 cut(s) 67, 1041, 1356
DdeI CTNAG 2 cut(s) 387, 1140
DpnI GATC 3 cut(s) 1073, 1395, 1581
DpnII GATC 3 cut(s) 1071, 1393, 1579
DraI TTTAAA 1 cut(s) 750
DrdI GACNNNNNNGTC 1 cut(s) 1292
DseDI GACNNNNNNGTC 1 cut(s) 1292
EaeI YGGCCR 2 cut(s) 312, 370
Eam1104I CTCTTC 3 cut(s) 207, 542, 1449
EarI CTCTTC 3 cut(s) 207, 542, 1449
Eco130I CCWWGG 4 cut(s) 586, 784, 1256, 1434
Eco24I GRGCYC 3 cut(s) 621, 1522, 1589
Eco32I GATATC 1 cut(s) 525
Eco47I GGWCC 4 cut(s) 308, 404, 743, 968
Eco47III AGCGCT 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 743
EcoRII CCWGG 2 cut(s) 372, 1483
EcoRV GATATC 1 cut(s) 525
EcoT14I CCWWGG 4 cut(s) 586, 784, 1256, 1434
EcoT38I GRGCYC 3 cut(s) 621, 1522, 1589
ErhI CCWWGG 4 cut(s) 586, 784, 1256, 1434
FalI AAGNNNNNCTT 2 cut(s) 986, 1018
FaqI GGGAC 2 cut(s) 417, 756
FokI GGATG 2 cut(s) 1099, 1258
FriOI GRGCYC 3 cut(s) 621, 1522, 1589
FspBI CTAG 2 cut(s) 378, 1532
GlaI GCGC 6 cut(s) 6, 44, 486, 600, 715, 1500
GsaI CCCAGC 1 cut(s) 618
HaeII RGCGCY 3 cut(s) 8, 46, 602
HaeIII GGCC 8 cut(s) 314, 337, 372, 619, 682, 783, 1001, 1178
HapII CCGG 3 cut(s) 311, 318, 407
HgaI GACGC 1 cut(s) 212
HhaI GCGC 6 cut(s) 7, 45, 487, 601, 716, 1501
Hin6I GCGC 6 cut(s) 5, 43, 485, 599, 714, 1499
HinP1I GCGC 6 cut(s) 5, 43, 485, 599, 714, 1499
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HinfI GANTC 5 cut(s) 346, 433, 664, 688, 734
HpaI GTTAAC 1 cut(s) 1159
HpaII CCGG 3 cut(s) 311, 318, 407
HphI GGTGA 4 cut(s) 17, 80, 295, 1115
Hpy166II GTNNAC 3 cut(s) 576, 1159, 1462
Hpy188I TCNGA 6 cut(s) 522, 564, 663, 891, 1541, 1576
Hpy188III TCNNGA 2 cut(s) 938, 1069
Hpy8I GTNNAC 3 cut(s) 576, 1159, 1462
Hpy99I CGWCG 1 cut(s) 1545
HpyAV CCTTC 6 cut(s) 66, 241, 280, 1012, 1168, 1411
HpyCH4III ACNGT 3 cut(s) 415, 925, 1452
HpyCH4IV ACGT 1 cut(s) 1390
HpyF3I CTNAG 2 cut(s) 387, 1140
HpySE526I ACGT 1 cut(s) 1390
HspAI GCGC 6 cut(s) 5, 43, 485, 599, 714, 1499
KspAI GTTAAC 1 cut(s) 1159
Kzo9I GATC 3 cut(s) 1071, 1393, 1579
LguI GCTCTTC 2 cut(s) 542, 1449
LmnI GCTCC 4 cut(s) 12, 262, 1211, 1572
Lsp1109I GCAGC 8 cut(s) 105, 382, 526, 656, 845, 853, 1173, 1475
LweI GCATC 4 cut(s) 526, 555, 860, 1296
MaeI CTAG 2 cut(s) 378, 1532
MaeII ACGT 1 cut(s) 1390
MaeIII GTNAC 5 cut(s) 104, 422, 810, 979, 1194
MalI GATC 3 cut(s) 1073, 1395, 1581
MbiI CCGCTC 1 cut(s) 1058
MboI GATC 3 cut(s) 1071, 1393, 1579
MboII GAAGA 7 cut(s) 224, 256, 409, 529, 1321, 1436, 1589
MflI RGATCY 1 cut(s) 1579
MhlI GDGCHC 5 cut(s) 621, 1376, 1444, 1522, 1589
MluCI AATT 2 cut(s) 581, 1203
MluI ACGCGT 1 cut(s) 204
MlyI GAGTC 1 cut(s) 355
MmeI TCCRAC 2 cut(s) 523, 1564
MnlI CCTC 9 cut(s) 38, 94, 208, 223, 244, 945, 1119, 1363, 1463
MseI TTAA 4 cut(s) 114, 749, 1158, 1618
MslI CAYNNNNRTG 1 cut(s) 296
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 3 cut(s) 311, 318, 407
MspR9I CCNGG 4 cut(s) 319, 374, 408, 1485
Mva1269I GAATGC 1 cut(s) 1188
MvaI CCWGG 2 cut(s) 374, 1485
MvnI CGCG 2 cut(s) 121, 206
NciI CCSGG 2 cut(s) 319, 408
NdeII GATC 3 cut(s) 1071, 1393, 1579
NheI GCTAGC 1 cut(s) 377
NlaIV GGNNCC 4 cut(s) 309, 405, 619, 744
NmuCI GTSAC 2 cut(s) 422, 1194
NspI RCATGY 2 cut(s) 811, 1567
NspV TTCGAA 1 cut(s) 242
PaeI GCATGC 1 cut(s) 1567
PagI TCATGA 1 cut(s) 1068
PaqCI CACCTGC 1 cut(s) 271
PciI ACATGT 1 cut(s) 807
PciSI GCTCTTC 2 cut(s) 542, 1449
PctI GAATGC 1 cut(s) 1188
PfeI GAWTC 4 cut(s) 433, 664, 688, 734
PflFI GACNNNGTC 1 cut(s) 293
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PpuMI RGGWCCY 1 cut(s) 743
PscI ACATGT 1 cut(s) 807
Psp5II RGGWCCY 1 cut(s) 743
Psp6I CCWGG 2 cut(s) 372, 1483
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 2 cut(s) 372, 1483
PspN4I GGNNCC 4 cut(s) 309, 405, 619, 744
PspOMI GGGCCC 1 cut(s) 617
PspPI GGNCC 8 cut(s) 308, 335, 404, 617, 618, 743, 968, 1177
PspPPI RGGWCCY 1 cut(s) 743
PstI CTGCAG 2 cut(s) 516, 646
PsuI RGATCY 1 cut(s) 1579
PsyI GACNNNGTC 1 cut(s) 293
RsaI GTAC 3 cut(s) 68, 1042, 1357
RsaNI GTAC 3 cut(s) 67, 1041, 1356
RseI CAYNNNNRTG 1 cut(s) 296
SapI GCTCTTC 2 cut(s) 542, 1449
SaqAI TTAA 4 cut(s) 114, 749, 1158, 1618
Sau3AI GATC 3 cut(s) 1071, 1393, 1579
Sau96I GGNCC 8 cut(s) 308, 335, 404, 617, 618, 743, 968, 1177
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 4 cut(s) 319, 374, 408, 1485
SduI GDGCHC 5 cut(s) 621, 1376, 1444, 1522, 1589
SfaNI GCATC 4 cut(s) 526, 555, 860, 1296
SfcI CTRYAG 2 cut(s) 512, 642
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 4 cut(s) 308, 404, 743, 968
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
SphI GCATGC 1 cut(s) 1567
Sse9I AATT 2 cut(s) 581, 1203
SsiI CCGC 5 cut(s) 91, 119, 192, 360, 1056
SspI AATATT 1 cut(s) 954
SspMI CTAG 2 cut(s) 378, 1532
StyD4I CCNGG 4 cut(s) 317, 372, 406, 1483
StyI CCWWGG 4 cut(s) 586, 784, 1256, 1434
TaaI ACNGT 3 cut(s) 415, 925, 1452
TaiI ACGT 1 cut(s) 1393
TaqI TCGA 3 cut(s) 242, 455, 1522
TasI AATT 2 cut(s) 581, 1203
TatI WGTACW 2 cut(s) 66, 1355
TauI GCSGC 2 cut(s) 121, 1058
TfiI GAWTC 4 cut(s) 433, 664, 688, 734
Tru1I TTAA 4 cut(s) 114, 749, 1158, 1618
Tru9I TTAA 4 cut(s) 114, 749, 1158, 1618
TscAI CASTG 5 cut(s) 487, 775, 928, 978, 1017
TseFI GTSAC 2 cut(s) 422, 1194
TseI GCWGC 8 cut(s) 93, 395, 514, 644, 833, 866, 1186, 1488
Tsp45I GTSAC 2 cut(s) 422, 1194
TspDTI ATGAA 6 cut(s) 433, 446, 582, 726, 743, 1393
TspGWI ACGGA 1 cut(s) 1167
TspRI CASTG 5 cut(s) 487, 775, 928, 978, 1017
Tth111I GACNNNGTC 1 cut(s) 293
VpaK11BI GGWCC 4 cut(s) 308, 404, 743, 968
XceI RCATGY 2 cut(s) 811, 1567
XspI CTAG 2 cut(s) 378, 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.