Rmu_ssc0000408.1_g000013

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000408.1
OG0019387
Physical Location & Seq
Reverse (-)
61204 .. 61994
791 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
atgttggcaatggcaacattcacaagctctgaagggcattcgcaaaactacgcggatgctttgtcgaagagtattttgttttccgaaggacagaggtcggaagttcaactttcccatggccttcacaacaacaatgtggtcatggagcgtgttggagttctggcaatctttaggtttggaactccagcatgcaaaggaatgggggacggatactttcttaaatccacaaacgttcctggttccgtggtaggcgttgttggtaacccaaatggcgactacacttgttgggaaagacccgaagacatggacactcctcgaaagtcctacatagtaacaaaacaaaagccaggctctgaagtctcggccgaaactgcagccgcccttgcagcttcttccatggtgtttagaggttcggacaacaggtcaacagacaagtatagaggatcttataacaatagcattcccaatggagcgtgcccgttttattgcgactacaatggatacatggatgagttggtttggggagctacgtggttgtacaaggcaacaaagagaccttactacaggagctatgtcaaacacaacatacataatttaggtagcgacgtttctcggggaacaaccctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

209

Amino Acids

22.99

Weight (kDa)

8.28

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 71 - 196 7.7e-32 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019387)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0156981
rosa_laevigata RLG00000020956
rosa_multiflora Rmu_ssc0000408.1_g000013
rosa_rugosa Rorug01G0059800 Rorug07G0215500
rosa_samantha Rh2AG523200 Rh2BG536400 Rh2DG545400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AccII CGCG 1 cut(s) 53
AciI CCGC 2 cut(s) 53, 378
AclI AACGTT 1 cut(s) 231
AclWI GGATC 1 cut(s) 451
AcoI YGGCCR 1 cut(s) 363
AcuI CTGAAG 2 cut(s) 51, 375
AfaI GTAC 1 cut(s) 539
AgsI TTSAA 1 cut(s) 107
AhdI GACNNNNNGTC 1 cut(s) 421
AjnI CCWGG 2 cut(s) 235, 346
AluBI AGCT 4 cut(s) 27, 389, 527, 570
AluI AGCT 4 cut(s) 27, 389, 527, 570
Alw26I GTCTC 2 cut(s) 364, 547
AlwI GGATC 1 cut(s) 451
AlwNI CAGNNNCTG 1 cut(s) 353
Ama87I CYCGRG 1 cut(s) 612
AoxI GGCC 2 cut(s) 118, 363
ApeKI GCWGC 2 cut(s) 374, 386
AvaI CYCGRG 1 cut(s) 612
BaeGI GKGCMC 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 306
BbvI GCAGC 2 cut(s) 386, 398
BciT130I CCWGG 2 cut(s) 237, 348
BciVI GTATCC 2 cut(s) 203, 494
BcoDI GTCTC 2 cut(s) 364, 547
BfmI CTRYAG 2 cut(s) 372, 562
BfuI GTATCC 2 cut(s) 203, 494
BisI GCNGC 3 cut(s) 375, 378, 387
BlsI GCNGC 3 cut(s) 376, 379, 388
Bme1390I CCNGG 2 cut(s) 237, 348
BmeRI GACNNNNNGTC 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 612
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 2 cut(s) 237, 348
BmsI GCATC 1 cut(s) 46
BoxI GACNNNNGTC 1 cut(s) 94
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 531
BsaI GGTCTC 1 cut(s) 547
BsaJI CCNNGG 3 cut(s) 115, 243, 396
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 2 cut(s) 237, 348
BseDI CCNNGG 3 cut(s) 115, 243, 396
BseGI GGATG 2 cut(s) 61, 514
BseMI GCAATG 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 303
BseSI GKGCMC 1 cut(s) 479
BseX3I CGGCCG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 386, 398
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 120, 365
BsiEI CGRYCG 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 612
BslFI GGGAC 1 cut(s) 218
BsmAI GTCTC 2 cut(s) 364, 547
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 2 cut(s) 37, 459
BsnI GGCC 2 cut(s) 120, 365
Bso31I GGTCTC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 612
Bsp1286I GDGCHC 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 537
Bsp143I GATC 1 cut(s) 443
Bsp19I CCATGG 2 cut(s) 115, 396
BspACI CCGC 2 cut(s) 53, 378
BspANI GGCC 2 cut(s) 120, 365
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 376
BspPI GGATC 1 cut(s) 451
BspTNI GGTCTC 1 cut(s) 547
BsrDI GCAATG 1 cut(s) 15
BsrGI TGTACA 1 cut(s) 537
BssECI CCNNGG 3 cut(s) 115, 243, 396
BssMI GATC 1 cut(s) 443
BssT1I CCWWGG 2 cut(s) 115, 396
Bst2UI CCWGG 2 cut(s) 237, 348
Bst6I CTCTTC 1 cut(s) 62
BstAUI TGTACA 1 cut(s) 537
BstBAI YACGTR 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 190, 475
BstDSI CCRYGG 3 cut(s) 115, 243, 396
BstEII GGTNACC 1 cut(s) 260
BstF5I GGATG 2 cut(s) 61, 514
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 1 cut(s) 446
BstMAI GTCTC 2 cut(s) 364, 547
BstMBI GATC 1 cut(s) 443
BstMCI CGRYCG 1 cut(s) 366
BstMWI GCNNNNNNNGC 3 cut(s) 371, 383, 386
BstNI CCWGG 2 cut(s) 237, 348
BstNSI RCATGY 1 cut(s) 192
BstPAI GACNNNNGTC 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 235, 346
BstSFI CTRYAG 2 cut(s) 372, 562
BstSLI GKGCMC 1 cut(s) 479
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 2 cut(s) 386, 398
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BstZI CGGCCG 1 cut(s) 363
BsuI GTATCC 2 cut(s) 203, 494
BsuRI GGCC 2 cut(s) 120, 365
BtgI CCRYGG 3 cut(s) 115, 243, 396
BtsCI GGATG 2 cut(s) 61, 514
Cac8I GCNNGC 2 cut(s) 190, 475
CaiI CAGNNNCTG 1 cut(s) 353
Csp6I GTAC 1 cut(s) 538
CviAII CATG 6 cut(s) 116, 142, 189, 304, 397, 505
CviJI RGCY 9 cut(s) 27, 120, 346, 351, 365, 377, 389, 527, 570
CviKI_1 RGCY 9 cut(s) 27, 120, 346, 351, 365, 377, 389, 527, 570
CviQI GTAC 1 cut(s) 538
DpnI GATC 1 cut(s) 445
DpnII GATC 1 cut(s) 443
DriI GACNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 363
EagI CGGCCG 1 cut(s) 363
Eam1104I CTCTTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 62
EclXI CGGCCG 1 cut(s) 363
Eco130I CCWWGG 2 cut(s) 115, 396
Eco31I GGTCTC 1 cut(s) 547
Eco52I CGGCCG 1 cut(s) 363
Eco57I CTGAAG 2 cut(s) 51, 375
Eco88I CYCGRG 1 cut(s) 612
Eco91I GGTNACC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 235, 346
EcoT14I CCWWGG 2 cut(s) 115, 396
ErhI CCWWGG 2 cut(s) 115, 396
FaeI CATG 6 cut(s) 119, 145, 192, 307, 400, 508
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FaqI GGGAC 1 cut(s) 218
FatI CATG 6 cut(s) 115, 141, 188, 303, 396, 504
Fnu4HI GCNGC 3 cut(s) 375, 378, 387
FokI GGATG 2 cut(s) 68, 521
Fsp4HI GCNGC 3 cut(s) 375, 378, 387
GluI GCNGC 3 cut(s) 375, 378, 387
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 2 cut(s) 120, 365
Hin1II CATG 6 cut(s) 119, 145, 192, 307, 400, 508
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
Hpy166II GTNNAC 1 cut(s) 426
Hpy188I TCNGA 5 cut(s) 31, 85, 100, 355, 415
Hpy8I GTNNAC 1 cut(s) 426
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 3 cut(s) 26, 80, 131
HpyCH4IV ACGT 3 cut(s) 231, 530, 606
HpyCH4V TGCA 3 cut(s) 192, 374, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 383, 386
HpySE526I ACGT 3 cut(s) 231, 530, 606
Hsp92II CATG 6 cut(s) 119, 145, 192, 307, 400, 508
Kzo9I GATC 1 cut(s) 443
LmnI GCTCC 4 cut(s) 145, 470, 524, 567
LpnPI CCDG 8 cut(s) 146, 198, 222, 249, 333, 360, 406, 550
Lsp1109I GCAGC 2 cut(s) 386, 398
LweI GCATC 1 cut(s) 46
MaeII ACGT 3 cut(s) 231, 530, 606
MaeIII GTNAC 2 cut(s) 260, 331
MalI GATC 1 cut(s) 445
MboI GATC 1 cut(s) 443
MboII GAAGA 3 cut(s) 79, 311, 384
MflI RGATCY 1 cut(s) 443
MhlI GDGCHC 1 cut(s) 479
MluCI AATT 1 cut(s) 592
MmeI TCCRAC 2 cut(s) 78, 133
MnlI CCTC 4 cut(s) 87, 324, 401, 434
MseI TTAA 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 237, 348
Mva1269I GAATGC 2 cut(s) 37, 459
MvaI CCWGG 2 cut(s) 237, 348
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 3 cut(s) 371, 383, 386
NcoI CCATGG 2 cut(s) 115, 396
NdeII GATC 1 cut(s) 443
NlaIII CATG 6 cut(s) 119, 145, 192, 307, 400, 508
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 1 cut(s) 341
NspI RCATGY 1 cut(s) 192
PaeI GCATGC 1 cut(s) 192
PctI GAATGC 2 cut(s) 37, 459
PkrI GCNGC 3 cut(s) 376, 379, 388
Ppu21I YACGTR 1 cut(s) 531
PshAI GACNNNNGTC 1 cut(s) 94
PsiI TTATAA 1 cut(s) 450
Psp1406I AACGTT 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 235, 346
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 2 cut(s) 235, 346
PspN4I GGNNCC 1 cut(s) 241
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 1 cut(s) 443
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 3 cut(s) 375, 378, 387
Sau3AI GATC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 237, 348
SduI GDGCHC 1 cut(s) 479
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 2 cut(s) 372, 562
SphI GCATGC 1 cut(s) 192
Sse9I AATT 1 cut(s) 592
SsiI CCGC 2 cut(s) 53, 378
StyD4I CCNGG 2 cut(s) 235, 346
StyI CCWWGG 2 cut(s) 115, 396
TaiI ACGT 3 cut(s) 234, 533, 609
TaqI TCGA 2 cut(s) 65, 316
TasI AATT 1 cut(s) 592
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 380
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseI GCWGC 2 cut(s) 374, 386
TspGWI ACGGA 2 cut(s) 222, 232
XceI RCATGY 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.